CN101411791B - Method for preparing total flavones from leaf of Belamcanda rhizome and use - Google Patents
Method for preparing total flavones from leaf of Belamcanda rhizome and use Download PDFInfo
- Publication number
- CN101411791B CN101411791B CN2008102275535A CN200810227553A CN101411791B CN 101411791 B CN101411791 B CN 101411791B CN 2008102275535 A CN2008102275535 A CN 2008102275535A CN 200810227553 A CN200810227553 A CN 200810227553A CN 101411791 B CN101411791 B CN 101411791B
- Authority
- CN
- China
- Prior art keywords
- total flavonoids
- leaf
- rhizoma belamcandae
- group
- diabetes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method and application for total flavonoids in Belamcanda chinensis leaves. The method comprises the following steps: (1) Belamcanda chinensis leaves are crushed into coarse powder for water extraction or alcohol extraction; and (2) an extracting solution passes through a macroporous absorbent resin column or a polyamide column, a method of the elution by water and 10 to 100 percent ethanol is adopted, an ethanol elution part is collected, and ethanol is recovered to be dry to obtain an extract of the total flavonoids in the Belamcanda chinensis leaves. The total flavonoids in the Belamcanda chinensis leaves can be used for preparing medicines for preventing and treating diabetes and complicating diseases of the diabetes. The invention has the advantages that the preparation method for the total flavonoids in the Belamcanda chinensis leaves adopts a method that an extraction method and a macroporous absorbent resin or polyamide are separated and used, the obtained extract of the total flavonoids in the Belamcanda chinensis leaves contains swertisin after the screening and optimization of technological parameters, and the content of the total flavonoids in the extract of the total flavonoids in the Belamcanda chinensis leaves is more than 55 percent (calculated as the swertisin); and the total flavonoids in the Belamcanda chinensis leaves can be developed into a novel medicine capable of synchronously preventing and treating the diabetes and the complicating diseases of the diabetes.
Description
Technical field
The present invention relates to a kind of Preparation method and use of Chinese medicine extract, particularly a kind of Preparation method and use of leaf of Rhizoma Belamcandae total flavonoids belongs to the processing and the purposes field of Chinese medicine, natural drug.
Background technology
Diabetes are one group of clinical syndromes that caused by the nature-nurture factor interaction, it is one group of common metabolism endocrinopathy, cause hyperglycemia with insulin secretion or effect defective or both common defects, it has long, the difficult radical cure of ill cycle, necessary long-term prescription, and the characteristics that very easily lead to complications, its main feature are hyperglycemia and glycosuria.Classical symptom can be summarized as " three-many-one-little ", i.e. polyuria, polydipsia, polyphagia and lose weight, and the later stage can be caused multiple syndrome.Along with the raising of people's living standard, the change of dietary structure, the reduction of labor intensity, the aggravation of aged tendency of population, the sickness rate of diabetes is the trend that rises gradually, and the city is significantly higher than the rural area.Interrelated data shows, the mortality rate that is caused by diabetes is higher at present, is only second to diseases such as cardiovascular, cerebrovascular and tumor, becomes to threaten human " No. three killer ", serious harm people's health.At present data shows that developed country's diabetes prevalence is about 3%～5%, and China's diabetes prevalence has also reached 1%～2%, but also with 1 ‰ speed cumulative year after year.Estimate that according to the relevant statistics of WHO in the period of nineteen ninety-five～2025, the number of patients in the worldwide will increase by 122%.
The drug main of treatment diabetes will be divided into synthetic drug and Chinese medicine, natural drug.
Synthetic drug comprises oral antidiabetic drug (chemical medicine) and insulin (bio-pharmaceuticals).Domestic oral antidiabetic drug commonly used can be divided into five classes at present, i.e. biguanides, sulfonylurea, alpha-glucosidase inhibitor, thiazolidinediones, meglitinide.The oral antidiabetic drug blood sugar lowering is rapid and effect is obvious, but certain side effect is all arranged, as hypoglycemia, digestive tract reaction, lactic acidosis etc.Successfully extract insulin from nineteen twenty-one, and since it was used for treating diabetes, insulin had played a part in treating diabetes very big from animal insulin.Insulinize can short-term be controlled metabolism disorder effectively when acute metabolism disorder, reduce case fatality rate.But its price is more expensive, side effect such as hypoglycemia, anaphylaxis, weight increase, immunologic insulin resistant, insulin edema, dioptric is not normal also might occur simultaneously.The symptom of existing in the market treatment diabetes Chinese medicine, the most energy of natural drug diabetes-alleviating, toxic and side effects is little, but blood sugar lowering is slow.Leaf of Rhizoma Belamcandae is the leaf of irides Rhizoma Belamcandae or Rhizoma Iridis Tectori, contains compositions such as flavone, alkaloid, phenolic acid, polysaccharide, has not yet to see about the extraction separation method of leaf of Rhizoma Belamcandae total flavonoids and the report of treatment diabetes and diabetic complication purposes thereof.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of leaf of Rhizoma Belamcandae total flavonoids, and the purposes that leaf of Rhizoma Belamcandae total flavonoids is used to prevent, prepare treatment diabetes and complication medicine aspect thereof.
The preparation method of leaf of Rhizoma Belamcandae total flavonoids comprises the steps:
(1) leaf of Rhizoma Belamcandae is crushed into coarse powder, carries out that water is carried or alcohol extraction, and water extraction is meant leaf of Rhizoma Belamcandae under the condition more than 50 ℃, is 1:2～1:50 extracting in water 0.5～5 hour by the percentage by weight of medical material and water, extracts 1～5 time, and extracting solution merges; Alcohol extracting method is meant leaf of Rhizoma Belamcandae under the condition more than 50 ℃, is that 1:2～1:50 adds 30%～95% ethanol extraction 0.5～5 hour by medical material and alcoholic acid percentage by weight, extracts 1～5 time, and extracting solution merges, decompression recycling ethanol;
(2) extracting solution is crossed macroporous adsorptive resins or polyamide column, adopts the way of water, 10%～100% ethanol elution, collects the ethanol elution part, reclaims ethanol to doing, and obtains the leaf of Rhizoma Belamcandae total flavonoids extract.Contain swertisin in the leaf of Rhizoma Belamcandae total flavonoids extract that obtains; In swertisin, general flavone content is more than 55% in the leaf of Rhizoma Belamcandae total flavonoids extract.
It is as follows that leaf of Rhizoma Belamcandae total flavonoids is used to prepare the purposes for the treatment of diabetes and complication medicine aspect thereof:
1. leaf of Rhizoma Belamcandae total flavonoids is to the influence of normal mouse blood sugar and serum insulin
Adopt normal mouse, give leaf of Rhizoma Belamcandae total flavonoids, measure fasting glucose and the mice serum insulin level of mice then, the result shows that each dosage group of leaf of Rhizoma Belamcandae total flavonoids and blank group relatively there is no significant difference, show that leaf of Rhizoma Belamcandae total flavonoids does not influence intact animal's blood glucose and insulin level.
2. leaf of Rhizoma Belamcandae total flavonoids is to the influence of alloxan diabetes mice fasting glucose
Get mice, the tail vein injection alloxan is made diabetes model, gets the mice of modeling success, gives leaf of Rhizoma Belamcandae total flavonoids, and the administration of weighing every day is surveyed blood glucose respectively at administration 10d, 20d docking blood sampling.Leaf of Rhizoma Belamcandae total flavonoids in 25～200mg/kg dosage range to the hyperglycemia animal administration due to the alloxan, compare with model control group, during administration 10d, significant decline (P＜0.05) promptly appears in 200mg/kg high dose group mouse blood sugar, during administration 20d, significantly descending (P＜0.05, P＜0.01) appears in 100～200mg/kg dosage group mouse blood sugar, and certain dose-response relationship is arranged, and the blood sugar lowering rate is 17.7%～24.4%.Show that leaf of Rhizoma Belamcandae total flavonoids has significant blood sugar reducing function to the zoic model with hyperglycemia due to the alloxan.
3. leaf of Rhizoma Belamcandae total flavonoids is to the influence of diabetes rat
The SD rat is divided into normal control group and modeling group (and establishing normal control group and model control group in addition) at random by body weight.After the modeling group adopts high-energy feed to raise 4w, the STZ of lumbar injection low dosage, blood is got in docking, and selecting blood glucose 〉=11.1mmol/L rat with the rapid blood sugar detector is modeling success rat.
Rat is divided at random by blood glucose in modeling: model control group, 4 dosage groups of metformin group (140mg/kg) and leaf of Rhizoma Belamcandae total flavonoids (140mg/kg, 70mg/kg, 35mg/kg, 17.5mg/kg), each is organized mean blood glucose and learns by statistics and handle difference and do not have significance (P〉0.05).Gastric infusion, normal control group and model control group are given the equal-volume solvent respectively.
(1) to the influence of blood glucose in diabetic rats
Leaf of Rhizoma Belamcandae total flavonoids is in dosage 70～140mg/kg scope the time, and the rat blood sugar significance reduces.Calculate the blood sugar lowering rate of each administration group, 70mg/kg, two dosage groups of 140mg/kg blood sugar reducing function are near 30%.With blood glucose the time is figure, visible administration group blood glucose curve obviously descends.Show that the reagent leaf of Rhizoma Belamcandae total flavonoids has the obvious functions of blood sugar effect to the type 2 diabetes mellitus rat.
(2) to the influence of diabetes rat carbohydrate tolerance
After each group gives glucose load after the modeling, the model group rat is significantly higher than normal control group (P＜0.01) at the blood glucose value of 0.5h, 1h, 2h each point, and the peak postpones, and shows that the normal matched group of glucose tolerance obviously reduces (P＜0.01), tangible impaired glucose tolerance occurred.
After leaf of Rhizoma Belamcandae total flavonoids treatment, in the time of in dosage 70～140mg/kg scope with model control group relatively, significance reduction (P＜0.05) promptly appears from 0.5h, continue to 2h still significance be lower than model control group (P＜0.05); Area under the calculated curve (AUC), the result shows that AUC significantly is lower than the model contrast in the time of in dosage 70～140mg/kg scope, and is tangible dosage correlation between each group, show that the reagent leaf of Rhizoma Belamcandae total flavonoids can significantly suppress the blood sugar increasing that type 2 diabetes mellitus model glucose load causes, improves carbohydrate tolerance.
(3) to the influence of diabetes rat TC, TG, HDL, LDL
The metabolism disorder of model group rat fat, after the leaf of Rhizoma Belamcandae total flavonoids treatment, when dosage 140mg/kg, can significantly reduce diabetes rat TC, when dosage 70～140mg/kg, can significantly reduce TG, four dosage group LDL and model control group more obviously reduce, and administration group HDL level is higher than model control group.
The invention has the advantages that the preparation method of described leaf of Rhizoma Belamcandae total flavonoids, the method that adopts extraction method and macroporous adsorbent resin, polyamide to separate logotype, through technological parameter screening, optimization, contain swertisin in the leaf of Rhizoma Belamcandae total flavonoids extract that obtains, in swertisin, general flavone content is more than 55% in the leaf of Rhizoma Belamcandae total flavonoids extract; Another advantage of the present invention is that the leaf of Rhizoma Belamcandae total flavonoids that obtains not only has good hypoglycemic activity, can prevent and treat diabetic complication again simultaneously, and can develop becomes a kind of newtype drug that has treatment diabetes and complication thereof concurrently.
The specific embodiment
Embodiment 1: the preparation of leaf of Rhizoma Belamcandae total flavonoids
(1) leaf of Rhizoma Belamcandae medical material 1kg is crushed into coarse powder, extracts; Add the 5L water boiling and extraction, extract 3 times, each 2 hours, extracting solution merged;
(2) extracting solution is crossed AB-8 type macroporous adsorptive resins absorption effective ingredient, discards effluent, continues and uses 70% ethanol elution, collects 70% ethanol elution, and decompression recycling ethanol obtains leaf of Rhizoma Belamcandae total flavonoids 65g to doing, and in swertisin, general flavone content is 60%.
Embodiment 2: the preparation of leaf of Rhizoma Belamcandae total flavonoids
(1) leaf of Rhizoma Belamcandae medical material 1kg is crushed into coarse powder, extracts; Add the 10L water boiling and extraction, extract 3 times, each 2 hours, extracting solution merged;
(2) extracting solution is crossed AB-8 type macroporous adsorptive resins absorption effective ingredient, discards effluent, continues and uses 70% ethanol elution, collects 70% ethanol elution, and decompression recycling ethanol obtains leaf of Rhizoma Belamcandae total flavonoids 70g to doing, and in swertisin, general flavone content is 63%.
Embodiment 3: the preparation of leaf of Rhizoma Belamcandae total flavonoids
(1) leaf of Rhizoma Belamcandae medical material 1kg is crushed into coarse powder, extracts; Add the 10L30% alcohol heating reflux and extract, extract 3 times, each 2 hours, extracting solution merged; Reclaim ethanol to there not being the alcohol flavor;
(2) extracting solution is crossed AB-8 type macroporous adsorptive resins absorption effective ingredient, discards effluent, continues and uses 70% ethanol elution, collects 70% ethanol elution, and decompression recycling ethanol obtains leaf of Rhizoma Belamcandae total flavonoids 70g to doing, and in swertisin, general flavone content is 61%.
Embodiment 4: the preparation of leaf of Rhizoma Belamcandae total flavonoids
(1) leaf of Rhizoma Belamcandae medical material 1kg is crushed into coarse powder, extracts; Add 5L 80% alcohol heating reflux and extract, extract 2 times, each 2 hours, extracting solution merged; Reclaim ethanol to there not being the alcohol flavor;
(2) extracting solution is crossed AB-8 type macroporous adsorptive resins absorption effective ingredient, discards effluent, continues and uses 70% ethanol elution, collects 70% ethanol elution, and decompression recycling ethanol obtains leaf of Rhizoma Belamcandae total flavonoids 78g to doing, and in swertisin, general flavone content is 65%.
Embodiment 5: the preparation of leaf of Rhizoma Belamcandae total flavonoids
(1) leaf of Rhizoma Belamcandae medical material 1kg is crushed into coarse powder, extracts; Add the 10L80% alcohol heating reflux and extract, extract 3 times, each 2 hours, extracting solution merged; Reclaim ethanol to there not being the alcohol flavor;
(2) extracting solution is crossed AB-8 type macroporous adsorptive resins absorption effective ingredient, discards effluent, continues and uses 70% ethanol elution, collects 70% ethanol elution, and decompression recycling ethanol obtains leaf of Rhizoma Belamcandae total flavonoids 80g to doing, and in swertisin, general flavone content is 62%.
Embodiment 6: the preparation of leaf of Rhizoma Belamcandae total flavonoids
(1) leaf of Rhizoma Belamcandae medical material 1kg is crushed into coarse powder, extracts; Add the 10L95% alcohol heating reflux and extract, extract 2 times, each 2 hours, extracting solution merged; Reclaim ethanol to there not being the alcohol flavor;
(2) extracting solution is crossed AB-8 type macroporous adsorptive resins absorption effective ingredient, discards effluent, continues and uses 70% ethanol elution, collects 70% ethanol elution, and decompression recycling ethanol obtains leaf of Rhizoma Belamcandae total flavonoids 76g to doing, and in swertisin, general flavone content is 68%.
The specific embodiment
Embodiment 1: leaf of Rhizoma Belamcandae total flavonoids is to the influence of normal mouse blood sugar and serum insulin
Experimentation:
With body weight 60 of the mices of 18～22g, male and female half and half, be divided into 6 groups at random, every group 10, be respectively the normal control group, positive controls glyburide (glibenclamide 50mg/kg) group, leaf of Rhizoma Belamcandae total flavonoids 200mg/kg, the 100mg/kg that embodiment 1 obtains, 50mg/kg, 25mg/kg dosage group, the normal control group gives solvent (0.5%CMC-Na), and each organizes equal gastric infusion, the administration volume is 10ml/kg, once a day, continuous 20 days, water 3-5h was can't help in the mice fasting before the last administration, 1h gets blood and surveys blood glucose after the last administration, gets blood afterwards and surveys serum insulin.
Experimental result:
After leaf of Rhizoma Belamcandae total flavonoids is given the continuous gastric infusion 20d of normal mouse, measure fasting blood sugar, each dosage group blood glucose and normal control group relatively there is no remarkable reduction or rising (P〉0.05); Detect and respectively to organize serum insulin levels with putting the method for exempting from, the result shows, each dosage group of leaf of Rhizoma Belamcandae total flavonoids and blank group relatively there is no significant difference (P〉0.05); Calculating insulin secretion index (IS, IS=FINS/FBG), each dosage group of leaf of Rhizoma Belamcandae total flavonoids and blank group unknown significance difference (P〉0.05).Show that leaf of Rhizoma Belamcandae total flavonoids does not influence intact animal's blood glucose and insulin secretion.The results are shown in Table 1.
Table 1 leaf of Rhizoma Belamcandae total flavonoids to the influence of normal mouse blood sugar, insulin and IS (X ± S, n=10)
Annotate: compare with matched group: * P＜0.05, * * P＜0.01
Embodiment 2: leaf of Rhizoma Belamcandae total flavonoids is to the influence of alloxan diabetes mice fasting glucose
Experimentation:
Behind 150 fasting of mice (can't help water) 24h of 18～22g, tail vein injection alloxan, alloxan dosage are 60mg/kg with body weight, and experimentation requires heating, and is sunny.Get blood in the 3rd day afterbody giving alloxan, survey fasting blood sugar behind fasting 3～5h with blood glucose meter, with fasting glucose 11.1mmol/L is as diabetic mice.
Select 60 mices of modeling success to be divided into 6 groups, be respectively oral four the dosage groups of leaf of Rhizoma Belamcandae total flavonoids (200mg/kg, 100mg/kg, 50mg/kg, 25mg/kg) that model control group, metformin group 190mg/kg, embodiment 1 obtain, every group 10, male and female half and half, other establishes one group of intact animal and makes blank, and matched group is given solvent (10ml/kg).Administration every day 1 time, successive administration 20d, the administration of weighing every day is surveyed blood glucose, routine observation mice food-intake and amount of drinking water respectively at administration 10d, 20d docking blood sampling.
Water 3～5h is can't help in the animal fasting before the experiment, surveys earlier fasting serum glucose (0h) during experiment, gastric infusion again, and matched group is given the equivalent solvent, gavages glucose (2g/kg) behind the 1h, measures the blood glucose (calculating AUC) of giving (0h) before the sugar and giving sugar back 0.5h, 1.0h, 2.0h.
Experimental result:
Leaf of Rhizoma Belamcandae total flavonoids in 25～200mg/kg dosage range to the hyperglycemia animal administration due to the alloxan, compare with model control group, during high dose group 200mg/kg administration 10d, significant decline (P＜0.05) promptly appears in mouse blood sugar, during administration 20d, significant decline (P＜0.05, P＜0.01) appears in 100～200mg/kg dosage group blood glucose, and certain dose-response relationship is arranged, and the blood sugar lowering rate is 17.7%～24.4%.Show that leaf of Rhizoma Belamcandae total flavonoids has significant blood sugar reducing function to the zoic model with hyperglycemia due to the alloxan.The results are shown in Table 2.
Table 2 leaf of Rhizoma Belamcandae total flavonoids extract is to the influence of alloxan diabetes mouse blood sugar (X ± S)
Administration group and model group compare: * P＜0.05, * * P＜0.01;
Embodiment 3 leaf of Rhizoma Belamcandae total flavonoids are to the influence of diabetes rat
Experimentation:
The SD rat is divided into normal control group (10) and modeling group (also establish normal control group and model control group in addition, 10 every group, monitor in order in the process of the test) at random by body weight.The modeling group adopts high-energy feed (10% sucrose, 0.3% cholate, 15% Adeps Sus domestica, 10% yolk powder, 64.7% normal feedstuff) raise 4w after, STZ (the 30mg/kg of lumbar injection low dosage, under the condition of ice bath,, now with the current) with the preparation of pH4.4 citric acid-sodium citrate buffer, blood is got in docking behind the 3d, selects blood glucose 〉=11.1mmol/L rat for becoming the mould rat with the rapid blood sugar detector.
Rat is divided at random by blood glucose in modeling: model control group, 4 dosage groups of the leaf of Rhizoma Belamcandae total flavonoids that metformin group (140mg/kg) and embodiment 1 obtain (140mg/kg, 70mg/kg, 35mg/kg, 17.5mg/kg), each is organized mean blood glucose and learns by statistics and handle difference and do not have significance (P〉0.05).Gastric infusion, normal control group and model control group are given the equal-volume solvent respectively, administration every day 1 time, 45d continuously.
Experimental result:
(1) to the influence of blood glucose in diabetic rats
Normal SD rats feed 4w with high lipid food and give with low dose of STZ modeling after, fasting glucose remarkable rising occurred and has continued to 45d, rat divides into groups by blood glucose before the administration, each organizes there was no significant difference between the blood glucose value (P〉0.05).Survey fasting glucose at 10d, 25d, 35d and 44d, the model group blood glucose value still is significantly higher than normally.
After the therapeutic administration 10 days, leaf of Rhizoma Belamcandae total flavonoids blood glucose in dosage 70～140mg/kg scope is starkly lower than model control group, but do not see biostatistics's difference (P〉0.05), behind the administration 25d, reduce (P＜0.05) at 140mg/kg dosage group rat blood sugar significance, continue to administration 44d, 70mg/kg dosage group rat blood sugar also significance reduces (P＜0.05).Calculate the blood sugar lowering rate of each administration group, 70mg/kg, two dosage groups of 140mg/kg blood sugar reducing function are near 30%.With blood glucose the time is figure, visible administration group blood glucose curve obviously descends.Show that the reagent leaf of Rhizoma Belamcandae total flavonoids has the obvious functions of blood sugar effect to the type 2 diabetes mellitus rat.The results are shown in Table 3.
Table 3-1 leaf of Rhizoma Belamcandae total flavonoids is to the influence of STZ type 2 diabetes mellitus rat blood sugar (X ± S)
Table 3-2 leaf of Rhizoma Belamcandae total flavonoids is to the influence of STZ type 2 diabetes mellitus rat blood sugar (X ± S)
Annotate: model group and matched group compare: #P＜0.05, ##P＜0.01;
Administration group and model group compare: * P＜0.05, * * P＜0.01;
(2) to the influence of diabetes rat carbohydrate tolerance
After each group gives glucose load behind the 44d after the modeling, the model group rat is significantly higher than normal control group (P＜0.01) at 0.5h, 1h, 2h each point blood glucose value, and the peak postpones, and shows that the normal matched group of glucose tolerance obviously reduces (P＜0.01), tangible impaired glucose tolerance occurred.
After leaf of Rhizoma Belamcandae total flavonoids treatment, when dosage 70～140mg/kg with model control group relatively, significance reduction (P＜0.05) promptly appears from 0.5h, continue to 2.0h still significance be lower than model control group (P＜0.05); Area under the calculated curve (AUC), the result shows that AUC significantly is lower than the model contrast when dosage 70～140mg/kg, and is tangible dosage correlation between each group, show that the reagent leaf of Rhizoma Belamcandae total flavonoids can significantly suppress the blood sugar increasing that type 2 diabetes mellitus model glucose load causes, improves carbohydrate tolerance.The results are shown in Table 4.
Table 4 leaf of Rhizoma Belamcandae total flavonoids extract is to the influence of STZ type 2 diabetes mellitus rat carbohydrate tolerance (X ± S)
Annotate: administration group and model group compare: * P＜0.05, * * P＜0.01;
Model and matched group compare: #P＜0.05, ##P＜0.01;
(3) to the influence of diabetes rat TC, TG, HDL, LDL
The metabolism disorder of model group rat fat, after the leaf of Rhizoma Belamcandae total flavonoids treatment, when dosage 140mg/kg, can significantly reduce diabetes rat TC, when dosage 70～140mg/kg, can significantly reduce TG, four dosage group LDL and model control group more obviously reduce, administration group HDL level is higher than model control group, but biostatistics's difference (P〉0.05) do not occur.
Table 5 leaf of Rhizoma Belamcandae total flavonoids is to the influence of diabetes rat TC, TG, HDL, LDL (X ± S)
Annotate: model group and matched group compare: #P＜0.05, ##P＜0.01;
Administration group and model group compare: * P＜0.05, * * P＜0.01.
Claims (1)
1. the purposes of a leaf of Rhizoma Belamcandae total flavonoids is characterized in that: leaf of Rhizoma Belamcandae total flavonoids reduces one of diabetic animal T-CHOL, triglyceride or low density lipoprotein, LDL, the application of the hdl level medicine of rising diabetic animal as preparation;
Wherein, described leaf of Rhizoma Belamcandae total flavonoids is prepared by following method:
(1) leaf of Rhizoma Belamcandae medical material 1kg is crushed into coarse powder, extracts; Add the 5L water boiling and extraction, extract 3 times, each 2 hours, extracting solution merged;
(2) extracting solution is crossed AB-8 type macroporous adsorptive resins absorption effective ingredient, discards effluent, continues and uses 70% ethanol elution, collects 70% ethanol elution, and decompression recycling ethanol obtains leaf of Rhizoma Belamcandae total flavonoids 65g to doing, and in swertisin, general flavone content is 60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102275535A CN101411791B (en) | 2008-11-27 | 2008-11-27 | Method for preparing total flavones from leaf of Belamcanda rhizome and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102275535A CN101411791B (en) | 2008-11-27 | 2008-11-27 | Method for preparing total flavones from leaf of Belamcanda rhizome and use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101411791A CN101411791A (en) | 2009-04-22 |
| CN101411791B true CN101411791B (en) | 2011-11-16 |
Family
ID=40592669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102275535A Active CN101411791B (en) | 2008-11-27 | 2008-11-27 | Method for preparing total flavones from leaf of Belamcanda rhizome and use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101411791B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050852B (en) * | 2009-07-07 | 2013-09-18 | 北京理工亘元医药技术开发中心有限公司 | 2'-O-rhamnosyl swertisin, preparation of analogues thereof, and application thereof |
| CN102688351A (en) * | 2011-03-23 | 2012-09-26 | 辽宁省中医药研究院 | Effective fractions of Belamcanda chinensis (L) DC., preparation method and application thereof |
| CN110755388B (en) * | 2019-12-05 | 2022-01-07 | 上海交通大学医学院附属新华医院 | Preparation process of blackberry lily granules |
| CN112826877A (en) * | 2021-01-19 | 2021-05-25 | 北京理工亘舒科技有限公司 | Belamcanda chinensis isolate, preparation method and application thereof |
-
2008
- 2008-11-27 CN CN2008102275535A patent/CN101411791B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN101411791A (en) | 2009-04-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102343023B (en) | Medicinal composition and preparation method and application thereof | |
| CN102526478B (en) | Formula of health-care medicine with functions of strengthening immunity and reducing blood sugar | |
| CN109549971A (en) | A kind of functional food and preparation method thereof adjusting hyperglycemia | |
| CN101878906A (en) | Health care food with auxiliary hyperglycemic function and preparation method thereof | |
| CN103223111B (en) | Traditional Chinese medicine composition for treating diabetic nephropathy and preparation method thereof | |
| CN101411791B (en) | Method for preparing total flavones from leaf of Belamcanda rhizome and use | |
| CN105030984B (en) | A kind of semen litchi health care oral liquid and preparation method with hypoglycemic, tune rouge and alleviation diabetic complication | |
| CN101390887A (en) | Medicine composition of Panax notoginseng saponins | |
| CN105687441A (en) | Application of litchi rind polyphenol in medicines for treating hyperglycemia and insulin resistance | |
| CN102885975B (en) | Traditional Chinese medicine composition for treating diabetes mellitus | |
| CN102697781B (en) | Application of trigonelline in preparation of medicament for preventing and treating diabetes and complication thereof | |
| CN1558768A (en) | A pharmaceutical composition made from Chinese traditional medicine and preparation method thereof | |
| CN102861201A (en) | Medicine composition for treating diabetes and preparation method thereof | |
| CN103919939A (en) | Application of japanese banana stem and leaf extract to preparation of medicament for treating or preventing diabetes | |
| CN101167781A (en) | Orally-administered hypoglycemic sweet potato leaf single prescription traditional Chinese medicine and preparation method thereof | |
| CN106214787B (en) | A kind of Chinese medicine composition for treating diabetes and preparation method thereof and purposes | |
| CN103432420B (en) | A kind of Chinese medicine composition for the treatment of diabetes and preparation method thereof and detection method | |
| CN102579530A (en) | Preparation method of aralia taibaiensis total saponin having diabetes mellitus resisting effect and medicament | |
| CN106265717B (en) | Application of the dicliptera chinensis polysaccharide in preparing prevention diabetes medicament or health products | |
| CN105106300A (en) | Use of cyclocarya paliurus extract in preparation of drug for preventing and treating non-alcoholic fatty liver disease | |
| CN100400081C (en) | Medicine for treating diabetes and production method thereof | |
| CN101474346A (en) | Longstamen onion bulb extract as well as preparation method and application thereof | |
| CN101254200B (en) | Use of cyclocarya paliurus glycoside compounds for preparing medicament for curing diabetes | |
| CN100534461C (en) | Pharmaceutical composition for treating diabetes and impaired glucose tolerance and preparation method thereof | |
| CN102240332A (en) | Pharmaceutical composition and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 100081 No. 5, Zhongguancun South Street, Haidian District, Beijing Patentee after: BEIJING INSTITUTE OF TECHNOLOGY Patentee after: BEIJING LIGONG GENYUAN TECHNOLOGY Co.,Ltd. Address before: 100081 No. 5, Zhongguancun South Street, Haidian District, Beijing Patentee before: BEIJING INSTITUTE OF TECHNOLOGY Patentee before: Beijing Science and Technology Genyuan Medicine Technology Development Co., Ltd. |