CN101398385A - Reagent for detecting copper in water and method for preparing same - Google Patents
Reagent for detecting copper in water and method for preparing same Download PDFInfo
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- CN101398385A CN101398385A CNA2007101572065A CN200710157206A CN101398385A CN 101398385 A CN101398385 A CN 101398385A CN A2007101572065 A CNA2007101572065 A CN A2007101572065A CN 200710157206 A CN200710157206 A CN 200710157206A CN 101398385 A CN101398385 A CN 101398385A
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Abstract
The invention relates to a measurement technique of heavy metal copper, in particular to a reagent used for measuring the copper in water and a preparation method thereof. According to weight parts, the reagent consists of 20-80 portions of chromogenic reagent, 50-200 portions of reducing agent, 100-700 portions of acidity regulator and 50-120 portions of masking agent; all compositions are sufficiently ground and uniformly mixed; and subsequently, the mixture is separately loaded in capsules with the quantity of 100-150mg per capsule and hermetically and dryly stored. The preparation method can simplify the analysis procedures, saves the operations such as weighing, liquid compounding and the like, is convenient and quick, reduces personal errors and can obtain exact results when the operations are carried out by non-analysis personnel. The reagent is solid powder capsule, and avoids instability of the liquid reagent; the hermetical shelf life under normal temperature is more than 6 months; furthermore, when the reagent is matched with a portable direct-reading colorimeter, once copper testing operation can be completed for 10-12 minutes.
Description
Technical field
The present invention relates to the determination techniques of heavy metal copper, especially a kind of reagent of measuring copper in the water quality and preparation method thereof.
Background technology
Copper is the life trace elements necessary, but excessive copper all is harmful to people and animal and plant.Total copper content is about 100～150mg among the normal human, and the activity and the incretory function of human body hematopoiesis, cell growth, some enzyme all played an important role.But excess intake, the system that then can stimulate digestion causes stomachache, vomiting.People's oral lethal dose is about 10g.Copper is bigger to unicellular lower eukaryote and crops toxicity, and its mass concentration reaches 0.1～0.2mg/L can make fish cause death, and toxicity can increase when coexisting with zinc. and bigger to shell aquatic organism toxicity, general fisheries water requires the mass concentration of copper below 0.01mg/L.For crops, copper is the heavy metal poisoning soprano, behind the plant absorbing copper ion. be fixed in the root cortex, influence Nutrient Absorption.When cupric is higher in the irrigation water, promptly in soil and crop, accumulate, can make crops withered.Copper has self-purification of waters and has a strong impact on, and when its mass concentration is 0.001mg/L, slight inhibiting effect is arranged promptly, when mass concentration is 0.01mg/L, obvious inhibiting effect is arranged.
China is in spot sampling, lab analysis stage basically to the check monitoring of copper at present.Analyzing and testing mainly is laboratory large-scale instrument and equipments such as atomic absorption spectrophotometer (AAS) with instrument, not only need the specialty analysis personnel operation, and analytical cycle is longer.In recent years direct-reading type, be the research focus in linear, field quick detection abroad with miniature instrument and supporting special agent always.These special agents cost an arm and a leg, and it is untimely to supply, and as long-term dependence on import, it is higher to detect cost, need a kind of have save time, laborsaving, cost-saving, can carry out the method for field quick detection heavy metal copper, to satisfy the needs of China's actual environment monitoring.
Summary of the invention
The object of the invention has been to provide that a kind of convenience is quick, cost is low, the reagent and preparation method thereof of copper in the mensuration water quality of good stability.
For achieving the above object, the technical solution used in the present invention is:
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 20～80 parts of developers, 50～200 parts of reductive agents, acidity regulator and 50～120 parts of screening agents of 100～700 parts are formed.
Described developer is 2,9-dimethyl-4,7 diphenyl-phenanthroline, 2, and 9-dimethyl-1,10 ferrosin, dibromo is to a chlorine chlorophosphonazo or a sulfonic acid azo antipyrine; Reductive agent is oxammonium hydrochloride, ascorbic acid or sodium sulphite; Acidity regulator is citric acid, Potassium Hydrogen Phthalate, sodium citrate or sodium hydrogen phosphate; Screening agent is ammonium citrate, EDTA or sodium chloride.
The preparation method: according to the parts by weight meter, take by weighing 20～80 parts of developers, 50～200 parts of reductive agents, 100～700 parts acidity regulator and 50～120 parts of screening agents are in mortar, fully grind, it is mixed after, with 100～150mg/ grain divide encapsulated in, hermetically drying is preserved, and is standby.
Described developer is 2,9-dimethyl-4,7 diphenyl-phenanthroline, 2, and 9-dimethyl-1,10 ferrosin, dibromo is to a chlorine chlorophosphonazo or a sulfonic acid azo antipyrine; Reductive agent is oxammonium hydrochloride, ascorbic acid or sodium sulphite; Acidity regulator is citric acid, Potassium Hydrogen Phthalate, sodium citrate or sodium hydrogen phosphate; Screening agent is ammonium citrate, EDTA or sodium chloride.
The advantage that the present invention had:
1. simple to operate.Adopt the present invention can simplify routine analyzer, as save operations such as weighing, solution allocation, fast convenient, reduced personal error, non-analysis personnel operation also can obtain accurate result.
2. good stability.Reagent of the present invention is the pressed powder capsule, has avoided the liquid reagent instability, and the normal temperature sealing was preserved the term of validity greater than 6 months.
3. fast convenient.Reagent of the present invention and portable direct-reading tintmeter are used, and only need 10～12min can finish the test operation of a copper; And reagent of the present invention is the pressed powder capsule, is easy to carry and tests.
4. inexpensive, be easy to get.The present invention measures that the reagent cost of copper is generally 20～30% of external imported product price in the water quality, and preparation is simple, is fit to China's actual conditions.
Embodiment
Embodiment 1
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 20 parts of developers, 200 parts of reductive agents, acidity regulator and 120 parts of screening agents of 100 parts are formed.
Described developer is 2,9-dimethyl-4,7 diphenyl-phenanthroline; Reductive agent is an oxammonium hydrochloride; Acidity regulator is a Potassium Hydrogen Phthalate; Screening agent is an ammonium citrate.
The preparation method: according to the parts by weight meter, take by weighing 20 parts of developers, 200 parts of reductive agents, 100 parts acidity regulator and 120 parts of screening agents are in mortar, fully grind, it is mixed after, with the 100mg/ grain divide encapsulated in, put into bottle, built-in drying agent, sealing is preserved.
Embodiment 2
Difference from Example 1:
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 80 parts of developers, 50 parts of reductive agents, acidity regulator and 50 parts of screening agents of 700 parts are formed.
Described developer is 2,9-dimethyl-1,10 ferrosin; Reductive agent is an ascorbic acid; Acidity regulator is a citric acid-sodium citrate; Screening agent is EDTA.
The preparation method is characterized in that: according to the parts by weight meter, take by weighing 80 parts of developers, 50 parts of reductive agents, 700 parts acidity regulator and 50 parts of screening agents fully grind in mortar, it is mixed after, with the 150mg/ grain divide encapsulated in, hermetically drying is preserved.
Embodiment 3
Difference from Example 1:
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 30 parts of developers, 70 parts of reductive agents, acidity regulator and 80 parts of screening agents of 200 parts are formed.
Described developer is that dibromo is to the chlorine chlorophosphonazo; Reductive agent is a sodium sulphite; Acidity regulator is a citric acid-sodium citrate; Screening agent is a sodium chloride.
The preparation method is characterized in that: according to the parts by weight meter, take by weighing 30 parts of developers, 70 parts of reductive agents, 200 parts acidity regulator and 80 parts of screening agents fully grind in mortar, it is mixed after, with the 130mg/ grain divide encapsulated in, hermetically drying is preserved.
Embodiment 4
Difference from Example 1:
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 40 parts of developers, 90 parts of reductive agents, acidity regulator and 90 parts of screening agents of 350 parts are formed.
Described developer be between sulfonic acid azo antipyrine; Reductive agent is an ascorbic acid; Acidity regulator is citric acid-sodium hydrogen phosphate; Screening agent is an ammonium citrate.
Embodiment 5
Difference from Example 1
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 65 parts of developers, 125 parts of reductive agents, acidity regulator and 70 parts of screening agents of 500 parts are formed.
Described developer is that dibromo is to the chlorine chlorophosphonazo; Reductive agent is a sodium sulphite; Acidity regulator is a Potassium Hydrogen Phthalate; Screening agent is a sodium chloride.
Embodiment 6
Difference from Example 1
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 75 parts of developers, 165 parts of reductive agents, acidity regulator and 105 parts of screening agents of 650 parts are formed.
Described developer be between sulfonic acid azo antipyrine; Reductive agent is a sodium sulphite; Acidity regulator is citric acid a--sodium hydrogen phosphate; Screening agent is ammonium citrate, EDTA or sodium chloride.
Embodiment 7
Difference from Example 1
Measure the reagent of copper in the water quality: according to the parts by weight meter, by 50 parts of developers, 190 parts of reductive agents, acidity regulator and 90 parts of screening agents of 600 parts are formed.
Described developer is 2,9-dimethyl-1,10 ferrosin; Reductive agent is a sodium sulphite; Acidity regulator is a citric acid-sodium citrate; Screening agent is ammonium citrate and sodium chloride.
Application examples
The reagent of copper is used with portable tintmeter in the mensuration water quality of employing the foregoing description, can realize the fast measuring of copper.
The concrete operations step is as follows:
Get 10mL water sample to be measured in cuvette, add 2mL alcohol or ketone solution, shake up, one of the reagent that adds copper in the described mensuration water quality of the foregoing description, fully thixotropy is left standstill 10min under the room temperature, puts into cell, under the designated program of portable tintmeter and wavelength, measure, directly read concentration value.
The precision of analysis result and accuracy:
Experiment shows, under 0.10～2.0mg/L mark-on concentration, and the recovery 85.6%～108.2%, five replicate determination results' of same water sample relative standard deviation is 1.90%～4.96%.
Actual water sample is measured: after getting five an amount of mark-ons of actual water sample, cooperate portable tintmeter to measure with special agent of the present invention, and compare with the measurement result of atomic absorption photometer.Experimental result sees Table 1.
Table 1 control experiment is mg/L as a result
| Tap water | Panjin Dongguo town water sample | Panjin gardening water | West, Huludao City 8,000 town's water samples | Shenyang Hun River water sample | |
| Adopt reagent of the present invention | 0.25 | 0.34 | 0.40 | 0.31 | 0.45 |
| Adopt atom absorption method | 0.28 | 0.30 | 0.42 | 0.30 | 0.40 |
Therefore the test result of the test result of special agent of the present invention and laboratory large-scale instrument and equipment atomic absorption spectrophotometer (AAS) has good comparison, is applied to the detection of water quality, can obtain quick, stable, reliable result.
Claims (4)
1. reagent of measuring copper in the water quality is characterized in that: according to the parts by weight meter, by 20～80 parts of developers, 50～200 parts of reductive agents, acidity regulator and 50～120 parts of screening agents of 100～700 parts are formed.
2. according to the reagent of copper in the described mensuration water quality of claim 1, it is characterized in that: described developer is 2,9-dimethyl-4,7 diphenyl-phenanthroline, 2, and 9-dimethyl-1,10 ferrosin, dibromo is to a chlorine chlorophosphonazo or a sulfonic acid azo antipyrine; Reductive agent is oxammonium hydrochloride, ascorbic acid or sodium sulphite; Acidity regulator is citric acid, Potassium Hydrogen Phthalate, sodium citrate or sodium hydrogen phosphate; Screening agent is ammonium citrate, EDTA or sodium chloride.
3. preparation method by the reagent of copper in the described mensuration water quality of claim 1, it is characterized in that: according to the parts by weight meter, take by weighing 20～80 parts of developers, 50～200 parts of reductive agents, 100～700 parts acidity regulator and 50～120 parts of screening agents are in mortar, fully grind, after it is mixed, with 100～150mg/ grain divide encapsulated in, hermetically drying is preserved.
4. according to the reagent of copper in the described mensuration water quality of claim 3, it is characterized in that: described developer is 2,9-dimethyl-4,7 diphenyl-phenanthroline, 2, and 9-dimethyl-1,10 ferrosin, dibromo is to a chlorine chlorophosphonazo or a sulfonic acid azo antipyrine; Reductive agent is oxammonium hydrochloride, ascorbic acid or sodium sulphite; Acidity regulator is citric acid, Potassium Hydrogen Phthalate, sodium citrate or sodium hydrogen phosphate; Screening agent is ammonium citrate, EDTA or sodium chloride.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101572065A CN101398385A (en) | 2007-09-29 | 2007-09-29 | Reagent for detecting copper in water and method for preparing same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101572065A CN101398385A (en) | 2007-09-29 | 2007-09-29 | Reagent for detecting copper in water and method for preparing same |
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| Publication Number | Publication Date |
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| CN101398385A true CN101398385A (en) | 2009-04-01 |
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| CNA2007101572065A Pending CN101398385A (en) | 2007-09-29 | 2007-09-29 | Reagent for detecting copper in water and method for preparing same |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101865854A (en) * | 2010-06-04 | 2010-10-20 | 广东环凯微生物科技有限公司 | Reagent for quickly determining copper concentration in water and use method thereof |
| CN102928353A (en) * | 2012-10-24 | 2013-02-13 | 同济大学 | Preparation method and using method for developing detection piece for determining copper ions in water |
| CN103033503A (en) * | 2012-12-10 | 2013-04-10 | 同济大学 | Preparation and application method of chromogenic detecting piece for determining iron in water |
| CN103063672A (en) * | 2011-10-19 | 2013-04-24 | 沈阳理工大学 | Rapid online analysis method for main component in acidic coppering electroplate liquid |
| CN103278457A (en) * | 2013-05-20 | 2013-09-04 | 同济大学 | Detection agent for determining content of nickel in water |
| CN104076027A (en) * | 2013-03-25 | 2014-10-01 | 内蒙古伊利实业集团股份有限公司 | Method for evaluating cleaning effect of food production equipment |
| CN104535503A (en) * | 2015-01-14 | 2015-04-22 | 同济大学 | Method for preparing and using detection agent for measuring content of cobalt in water body |
| CN113504190A (en) * | 2021-06-30 | 2021-10-15 | 广东环凯生物技术有限公司 | Rapid detection method and reagent for water quality trace copper |
-
2007
- 2007-09-29 CN CNA2007101572065A patent/CN101398385A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101865854A (en) * | 2010-06-04 | 2010-10-20 | 广东环凯微生物科技有限公司 | Reagent for quickly determining copper concentration in water and use method thereof |
| CN103063672A (en) * | 2011-10-19 | 2013-04-24 | 沈阳理工大学 | Rapid online analysis method for main component in acidic coppering electroplate liquid |
| CN102928353A (en) * | 2012-10-24 | 2013-02-13 | 同济大学 | Preparation method and using method for developing detection piece for determining copper ions in water |
| CN102928353B (en) * | 2012-10-24 | 2014-10-22 | 同济大学 | Preparation method and using method for developing detection piece for determining copper ions in water |
| CN103033503A (en) * | 2012-12-10 | 2013-04-10 | 同济大学 | Preparation and application method of chromogenic detecting piece for determining iron in water |
| CN104076027A (en) * | 2013-03-25 | 2014-10-01 | 内蒙古伊利实业集团股份有限公司 | Method for evaluating cleaning effect of food production equipment |
| CN103278457A (en) * | 2013-05-20 | 2013-09-04 | 同济大学 | Detection agent for determining content of nickel in water |
| CN104535503A (en) * | 2015-01-14 | 2015-04-22 | 同济大学 | Method for preparing and using detection agent for measuring content of cobalt in water body |
| CN113504190A (en) * | 2021-06-30 | 2021-10-15 | 广东环凯生物技术有限公司 | Rapid detection method and reagent for water quality trace copper |
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Open date: 20090401 |