CN101390636B - Monascus vinegar beverage with blood fat reduction function and preparation method thereof - Google Patents
Monascus vinegar beverage with blood fat reduction function and preparation method thereof Download PDFInfo
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Abstract
The invention relates to monascus vinegar drink which has the capability of reducing blood fat and a preparation method thereof. The monascus vinegar drink is prepared through the following method: 1) preparation of hawkthorn and medlar liquid extract: mixing hawkthorn and medlar, decocting through water to extract, filtrating the liquid extracts, depositing the filtrate, and filtrating again; 2)preparation of monascus vinegar: washing and grinding rice, soaking the rice in water, steaming the rice, inoculating monascus to the rice and adding functional red yeast rice for fermentation, then liquefying, saccharifying, fermenting through acetic acid bacteria and filtrating sequentially; 3) preparation of vinegar drink: adding water to the hawkthorn and medlar liquid extracts to dilute the liquid extracts, adding monascus vinegar to adjust the pH of the drink, adding honey and then adding white granulated sugar, and finally sterilizing. The drink has cheap material, simple formula and preparation process and not only has the unique flavor of vinegar drink and but also has the function of reducing blood fat.
Description
Technical field:
The present invention relates to plant and have functional food of effect for reducing blood fat and preparation method thereof.
Background technology:
Monascus is Eumycota, Ascomycetes, Euascomycetae, Aspergillales, Aspergillaceae fungi.What be used to prepare edible red colouring agent for food, also used as a Chinese medicine at present mainly is monascus parpureus Went.Red colouring agent for food, also used as a Chinese medicine is that monascus is inoculated in the rice top fermentation that cooks and forms, and its look crimson, is exactly the good merchantable brand of medicine-food two-purpose since ancient times, has characteristics warm in nature, that flavor is sweet, nontoxic, can be used for producing and brewages wine of rice fermented with red yeast, monascus vinegar, fermented bean curd, natural colouring matter etc.All the medical application of red colouring agent for food, also used as a Chinese medicine there is write up in " the Amplification on Materia Medica addendum " of Yuan Dynasty well-known doctor Zhu Danxi and the Compendium of Material Medica of Ming Dynasty's Li Shizhen (1518-1593 A.D.).After 1979 discover that can produce a kind of very strong lipopenicillinase active material-Luo in the red koji fermentation process cuts down its spit of fland, cut down the lipopenicillinase medicine that its spit of fland is a primary raw material with domestic the appearance in succession with the Lip river in the world.China has the record of making monascus vinegar very early in the manufacture craft of vinegar, along with the continuous application of modern biotechnology and industrial technology, the functional study of vinegar also more and more receives people's attention.Vinegar beverage begins to occur in the international market as a kind of novel drink in recent years.Vinegar beverage just receives consumer's welcome because of its unique taste, abundant nutritive value and plurality of health care functions once listing, and its market prospects are noticeable.Functional component content is lower in the common vinegar beverage but the problem that exists at present is, and the intake of people every day can not be too many.Therefore make common vinegar beverage be difficult to zoopery evaluation, and can not reach the requirement of " health food " through strictness.
Summary of the invention
The present invention seeks to provides a kind of monascus vinegar beverage with effect for reducing blood fat and preparation method thereof in order to address the above problem.
For achieving the above object, the present invention adopts following technical scheme:
A kind of monascus vinegar beverage that effect for reducing blood fat is arranged, it can be prepared by following method: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution:
Hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more;
Wherein: the ratio of hawthorn and matrimony vine is 1: 0.5-2; The volume of institute's water is hawthorn, matrimony vine mixture 2-3 a times;
2), the preparation of monascus vinegar:
Rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter;
Wherein, the addition that adds functional red yeast rice rice fermentation is that its spit of fland content 100-200mg/L is cut down in the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage:
Thin up in hawthorn, fructus lycii extracted solution adds monascus vinegar adjustment beverage pH value then, adds honey thereafter, adds white granulated sugar again, then sterilization;
Wherein, the ratio of thin up extract is for containing two kinds of crude drugs 30-60 gram altogether according to every 1000ml beverage, and adjusted beverage pH value is pH3-4; The total content that adds honey and white granulated sugar is 6%-10%, and honey: white granulated sugar is 1: 2.5-1: 5.
In the preparation of hawthorn, fructus lycii extracted solution: the filtrating extraction time can be twice, can be 30min at every turn; After merging the soup and filtration of extracted twice, can be 12-24 hour the standing time of will filtrating.
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine can be 1: 0.5; The volume of institute's water can be hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition can cut down its spit of fland content 100mg/L for the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 30 grams according to every 1000ml beverage; Adjusted beverage pH value is pH4; Thereafter the content that adds honey can contain honey 10g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 50g for every 1000ml.
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine can be 1: 1; The volume of institute's water can be hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition can cut down its spit of fland content 100mg/L for the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 40 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.5; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 60g for every 1000ml.
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine can be 1: 1.5; The volume of institute's water can be hawthorn, matrimony vine mixture 3 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition can cut down its spit of fland content 150mg/L for the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 50 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.2; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
A kind of monascus vinegar beverage that effect for reducing blood fat is arranged, it can be prepared by following method: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution: hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more; Wherein: the ratio of hawthorn and matrimony vine is 1: 2; The volume of institute's water is hawthorn, matrimony vine mixture 3 times;
2), the preparation of monascus vinegar: rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter; Wherein, the addition that adds functional red yeast rice rice fermentation is that its spit of fland content 200mg/L is cut down in the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage: thin up in hawthorn, fructus lycii extracted solution, add monascus vinegar adjustment beverage pH value then, add honey thereafter, add white granulated sugar again, sterilize then; Wherein, the ratio of thin up extract is for containing two kinds of crude drugs totally 60 grams according to every 1000ml beverage, and adjusted beverage pH value is pH3.0; Thereafter its content that adds honey can contain honey 30g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
A kind of preparation method that the monascus vinegar beverage of effect for reducing blood fat is arranged, it may further comprise the steps: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution: hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more; Wherein: the ratio of hawthorn and matrimony vine can be 1: 0.5-2; The volume of institute's water can be hawthorn, matrimony vine mixture 2-3 times;
2), the preparation of monascus vinegar: rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter; Wherein, the addition that adds functional red yeast rice rice fermentation can cut down its spit of fland content 100-200mg/L for the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage: thin up in hawthorn, fructus lycii extracted solution, add monascus vinegar adjustment beverage pH value then, add honey thereafter, add white granulated sugar again, sterilize then; Wherein, the ratio of thin up extract is for containing two kinds of crude drugs 30-60 gram altogether according to every 1000ml beverage, and adjusted beverage pH value is pH3-4; The total content that adds honey and white granulated sugar is 6%-10%, and honey: white granulated sugar is 1: 2.5-5.
In the preparation of hawthorn, fructus lycii extracted solution: the filtrating extraction time can be twice, each 30min; After merging the soup and filtration of extracted twice, can be 12-24 hour the standing time of will filtrating.
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine can be 1: 0.5; The volume of institute's water can be hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition can cut down its spit of fland content 100mg/L for the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 30 grams according to every 1000ml beverage; Adjusted beverage pH value is pH4; Thereafter its content that adds honey can contain honey 10g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 50g for every 1000ml.
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine can be 1: 1; The volume of institute's water can be hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition can cut down its spit of fland content 100mg/L for the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 40 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.5; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 60g for every 1000ml.
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine can be 1: 1.5; The volume of institute's water can be hawthorn, matrimony vine mixture 3 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition can cut down its spit of fland content 150mg/L for the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 50 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.2; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
A kind of preparation method that the monascus vinegar beverage of effect for reducing blood fat is arranged is characterized in that it may further comprise the steps: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution: hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more; Wherein: the ratio of hawthorn and matrimony vine is 1: 2; The volume of institute's water is hawthorn, matrimony vine mixture 3 times;
2), the preparation of monascus vinegar: rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter; Wherein, the addition that adds functional red yeast rice rice fermentation is that its spit of fland content 200mg/L is cut down in the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage: thin up in hawthorn, fructus lycii extracted solution, add monascus vinegar adjustment beverage pH value then, add honey thereafter, add white granulated sugar again, sterilize then; Wherein, the ratio of thin up extract is for containing two kinds of crude drugs totally 60 grams according to every 1000ml beverage, and adjusted beverage pH value is pH3.0; Thereafter its content that adds honey can contain honey 30g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
To taking of the above-mentioned monascus vinegar beverage that effect for reducing blood fat arranged, it is 2 each 250ml every day that the adult recommends dose.
Beneficial effect of the present invention is:
The present invention is a base stock with the monascus vinegar that contains lipopenicillinase active material-Luo and cut down its spit of fland; Be equipped with medicinal and edible Chinese medicine: hawthorn and matrimony vine with lipid-reducing function; Prepare a kind of monascus vinegar beverage with hypolipemic function; And through in " health food detects and the assessment technique standard " according to State Food and Drug Administration's regulation the evaluation method of blood fat reducing healthcare food being carried out zoopery to monascus vinegar beverage, the result proves that monascus vinegar beverage of the present invention has hypolipemic function.So not only raw material is cheap, the preparation method is simple in the present invention, and the peculiar flavour of the existing vinegar beverage of drinks of preparation, make it possess hypolipemic function again.
Specify the present invention through embodiment below.
The specific embodiment
Preparation embodiment 1 has the preparation of the monascus vinegar beverage of effect for reducing blood fat to comprise the steps:
1) gets hawthorn+matrimony vine (1: 0.5) and extract twice, each 30min → merging soup → filtration → 12 hours → filtration of placement with 2 times of volume decoctings.
2) get rice → cleaning → pulverizing → immersion → steamed rice → inoculation monascus (5%) and add functional red yeast rice rice (6%, cutting down its spit of fland content through the total Lip river of detection monascus vinegar is 100mg/L) fermentation → liquefaction → saccharification → acetic acid bacteria fermentation → filtration.
3) allotment of vinegar beverage: contain crude drug totally 30 gram thin up extracts → adding monascus vinegar adjustment beverage pH value according to every 1000ml beverage; Make it reach pH4.0 → adding honey; Making its content is that every 1000ml contains honey 10g → adding white granulated sugar, and making its content is that every 1000ml contains granulated sugar 50g → sterilization → bottling.
Preparation embodiment 2 has the preparation of the monascus vinegar beverage of effect for reducing blood fat to comprise the steps:
1) gets hawthorn+matrimony vine (1: 1) and extract twice, each 30min → merging soup → filtration → 12 hours → filtration of placement with 2 times of volume decoctings.
2) get rice → cleaning → pulverizing → immersion → steamed rice → inoculation monascus (5%) and add functional red yeast rice rice (6%, cutting down its spit of fland content through the total Lip river of detection monascus vinegar is 100mg/L) fermentation → liquefaction → saccharification → acetic acid bacteria fermentation → filtration.
3) allotment of vinegar beverage: contain crude drug totally 40 gram thin up extracts → adding monascus vinegar adjustment beverage pH value according to every 1000ml beverage; Make it reach pH3.5 → adding honey; Making its content is that every 1000ml contains honey 20g → adding white granulated sugar, and making its content is that every 1000ml contains granulated sugar 60g → sterilization → bottling.
Preparation embodiment 3 has the preparation of the monascus vinegar beverage of effect for reducing blood fat to comprise the steps:
1) gets hawthorn+matrimony vine (1: 1.5) and extract twice, each 30min → merging soup → filtration → 24 hours → filtration of placement with 3 times of volume decoctings.
2) get rice → cleaning → pulverizing → immersion → steamed rice → inoculation monascus (5%) and add functional red yeast rice rice (9%, cutting down its spit of fland content through the total Lip river of detection monascus vinegar is 150mg/L) fermentation → liquefaction → saccharification → acetic acid bacteria fermentation → filtration.
3) allotment of vinegar beverage: contain crude drug totally 50 gram thin up extracts → adding monascus vinegar adjustment beverage pH value according to every 1000ml beverage; Make it reach pH3.2 → adding honey; Making its content is that every 1000ml contains honey 20g → adding white granulated sugar, and making its content is that every 1000ml contains granulated sugar 70g → sterilization → bottling.
Preparation embodiment 4 has the preparation of the monascus vinegar beverage of effect for reducing blood fat to comprise the steps:
1) gets hawthorn+matrimony vine (1: 2) and extract twice, each 30min → merging soup → filtration → 24 hours → filtration of placement with 3 times of volume decoctings.
2) get rice → cleaning → pulverizing → immersion → steamed rice → inoculation monascus (5%) and add functional red yeast rice rice (12%, cutting down its spit of fland content through the total Lip river of detection monascus vinegar is 200mg/L) fermentation → liquefaction → saccharification → acetic acid bacteria fermentation → filtration.
3) allotment of vinegar beverage: contain crude drug totally 60 gram thin up extracts → adding monascus vinegar adjustment beverage pH value according to every 1000ml beverage; Make it reach pH3 → adding honey; Making its content is that every 1000ml contains honey 30g → adding white granulated sugar, and making its content is that every 1000ml contains granulated sugar 70g → sterilization → bottling.
Detect the proof experiment of embodiment 1 hypolipemic function of the present invention
The monascus vinegar beverage with effect for reducing blood fat that this test is used is that above-mentioned preparation embodiment 1 is obtained.
Test basis: health food check and assessment technique standard (Ministry of Public Health 2003 editions).
1. experiment material and method
1.1 experimental subjects
Select healthy 60 of the level male rats that clean of 150-200gWistar of Military Medical Science Institute's Experimental Animal Center [credit number: SCXK-(army) 2002-001] breeding for use.
1.2 laboratory apparatus and reagent
1.2.1 instrument
755 type spectrophotometers, thermostat water bath, centrifuge
1.2.2 reagent
T-CHOL kit (the middle north control biotechnology limited company of giving birth to)
Triglycerides kit (the middle north control biotechnology limited company of giving birth to)
HDL-C kit (the middle north control biotechnology limited company of giving birth to)
1.3 high lipid food prescription
78.8% basal feed, 1% cholesterol, 10% yolk powder, 10% lard, 0.2% cholate.
1.4 animal divides into groups and feeds
Rat gave the arm's length basis feed after 7 days, fasting 12 hours, eye socket is got hematometry serum total cholesterol (TC), triglyceride TG) and HDL-C (HDL-C).Be divided into 3 groups at random according to the TC level: control group, low dose group and high dose group, 12 every group.Low dose group is irritated stomach after concentrating 5 times with former beverage decompression.High dose group is irritated stomach after concentrating 8 times with former beverage decompression.High fat control group water (sterilizing) replaces being tried thing, and it is long-pending with respectively to be tried the thing group identical to irritate body of stomach every day.The rat oral gavage volume is the 0.8ml/100g body weight.Tried thing continuously after 60 days, fasting 12 hours is measured and is respectively organized rat blood serum T-CHOL, triglyceride and HDL-C
1.5 detection method
1.5.1 serum total cholesterol determination method
Get blood, 3000r/min isolated serum in centrifugal 10 minutes, the according to the form below operation:
| ? | Blank pipe | Standard pipe | Sample cell |
| Working solution (mL) | 2.00 | ?2.00 | ?2.00 |
[0092]
| Redistilled water (μ L) | 20.0 | - | - |
| Definite value serum (μ L) | - | 20.0 | - |
| Serum (μ L) | - | - | 20.0 |
Behind the mixing, 37 ℃ are incubated 6 minutes, measure in the 500nm wavelength, read the absorbance A definite value serum and the A sample of relative reagent blank respectively.Be calculated as follows:
1.5.2 triglyceride determination method
Get blood, 3000r/min isolated serum in centrifugal 10 minutes, the according to the form below operation:
| ? | Blank pipe | Standard pipe | Sample cell |
| Working solution (mL) | 2.00 | 2.00 | 2.00 |
| Redistilled water (μ L) | 20.0 | - | - |
| Standard (μ L) | - | 20.0 | - |
| Serum (μ L) | - | - | 20.0 |
Behind the mixing, 37 ℃ are incubated 10 minutes, measure in the 500nm wavelength, read the absorbance A of relative reagent blank respectively
StandardAnd A
SampleBe calculated as follows:
1.5.3 determine cholesterol with high density lipoprotein method
Get blood, 3000r/min isolated serum in centrifugal 10 minutes, and serum is mixed by 1: 1 with precipitating reagent, and room temperature was placed 10 minutes, and centrifugal 15 minutes of 3000r/min gets the operation of supernatant according to the form below:
| ? | Blank pipe | Standard pipe | Sample cell |
| Working solution (mL) | 2.00 | ?2.00 | 2.00 |
| Redistilled water (μ L) | 50.0 | ?- | - |
| Definite value serum (μ L) | - | ?50.0 | - |
| Supernatant (μ L) | - | ?- | 50.0 |
Behind the mixing, 37 ℃ are incubated 6 minutes, measure in the 500nm wavelength, read the absorbance A of relative reagent blank respectively
Definite value serumAnd A
SampleBe calculated as follows:
1.6 the processing of experimental data
Statistical method: experimental data is carried out the variance analysis statistics with Excel software.
2. experimental result
2.1 monascus vinegar beverage is to the influence of rat body weight
Table 1 monascus vinegar beverage is to the influence
of rat body weight
| Group | Number of animals (only) | Give and tried the preceding body weight (g) of thing | Give and tried the preceding P value of thing | Give and to be tried thing body weight (g) after 60 days | Give and tried the P value of thing after 60 days |
| High fat control group | 12 | 234±15 | - | 488±22 | - |
| Low dose group | 12 | 230±10 | 0.372575 | 479±16 | 0.286862 |
| High dose group | 12 | 266±10 | 0.132803 | 475±18 | 0.118823 |
Visible by table 1, rat is given and is tried respectively to organize body weight before the thing and do not have significant difference.Give and to be tried thing and respectively organize body weight after 60 days and significant difference (P＞0.05) also do not occur.Show that monascus vinegar beverage does not have harmful effect to rat growthing development.
2.2 monascus vinegar beverage is to the influence of Serum TC
Table 2 monascus vinegar beverage is to the influence
of Serum TC
| Group | Number of animals (only) | ?TC(mmol/L | ? | ? | ? |
| To before being tried thing | The P value | Tried thing after 60 days | The P value | ? | ? |
| High fat control group | 12 | ?1.66±0.22 | ?- | ?2.09±0.20 | ?0.000014?* |
| Low dose group | 12 | ?1.68±0.14 | ?0.807324 | ?1.93±0.21 | ?0.074715 |
| High dose group | 12 | ?1.67±0.17 | ?0.934630 | ?1.90±0.14 | ?0.059009 |
* give with this group and tried to compare before the thing.
Visible by table 2, tried relatively there was no significant difference (P＞0.05) of preceding each experimental group serum TC of thing and high fat control group.Tried thing after 60 days, relatively, serum TC raises before the experiment of high fat control group and this group, and difference has utmost point conspicuousness (P＜0.01), shows that high TC model sets up successfully.With high fat control group relatively, two dose groups serum total cholesterols decrease, but obvious difference (P＞0.05) not as yet.
2.3 monascus vinegar beverage is to the influence of rat blood serum TG
Table 3 monascus vinegar beverage is to the influence
of rat blood serum TG
| Group | Number of animals (only) | TG(mmol/L) | ? | ? | ? |
| To before being tried thing | The P value | Tried thing after 60 days | The P value | ? | ? |
| High fat control group | 12 | 0.69±0.12 | - | 0.94±0.13 | 0.000067* |
[0122]
| Low dose group | 12 | 0.71 ± 0.15 | 0.80732 4 | 0.79 ± 0.16 | 0.021986 |
| High dose group | 12 | 0.70 ± 0.19 | 0.93463 | 0.69 ± 0.121 | 0.000111 |
* give with this group and tried to compare before the thing.
Visible by table 3, tried preceding each experimental group serum TG of thing and high fat control group relatively, difference that there are no significant (P＞0.05).Tried thing after 60 days, the contrast of high fat vertical with this group experiment before relatively, the serum TG rising, difference has utmost point conspicuousness (P＜0.01), shows that high TG model sets up successfully.Compare with high fat control group, two equal conspicuousnesses of dose groups serum triglyceride reduce.Wherein low dose group has reduced by 16% (P＜0.05); High dose group has reduced by 26% (P＜0.01) and has explained that monascus vinegar beverage has the effect that reduces serum triglyceride content.
2.4 monascus vinegar beverage is to the influence of rat blood serum HDL-C
Table 4 monascus vinegar beverage is to the influence
of rat blood serum HDL-C
| Group | Number of animals (only) | ?HDL-C(mmol/L) | ? | ? | ? |
| To before being tried thing | The P value | Tried thing after 60 days | The P value | ? | ? |
| High fat control group | 12 | ?0.96±0.19 | ?- | ?0.865±?0.154 | ?- |
| Low dose group | 12 | ?0.99±0.13 | ?0.7448?11 | ?0.932±?0.180 | ?0.3392?88 |
| High dose group | 12 | ?0.98±0.12 | ?0.8296?87 | ?0.950±?0.204 | ?0.2607?39 |
Visible by table 4, tried thing after 60 days, with high fat control group relatively, two dose groups serum high-density LP cholesterol levels are obvious difference (P＞0.05) not.Explain that monascus vinegar beverage does not have the effect that improves the serum high-density LP cholesterol level.
3. conclusion
In sum, can know the criterion of health-care food for assisting blood fat lowering, adopt preparation embodiment 1 prepared monascus vinegar beverage to have the effect that reduces serum triglyceride content according to China's " health food check and assessment technique standard " (2003 editions).
Detect the proof experiment of embodiment 2 hypolipemic functions of the present invention
The monascus vinegar beverage with effect for reducing blood fat that this test is used is that above-mentioned preparation embodiment 4 is obtained.
Test basis: health food check and assessment technique standard (Ministry of Public Health 2003 editions).
1. experiment material and method
1.1 experimental subjects
With detecting embodiment 1
1.2 laboratory apparatus and reagent
With detecting embodiment 1
1.3 high lipid food prescription
With detecting embodiment 1
1.4 animal divides into groups and feeds
With detecting embodiment 1
1.5 detection method
With detecting embodiment 1
1.6 the processing of experimental data
With detecting embodiment 1
2. experimental result
2.1 monascus vinegar beverage is to the influence of rat body weight
Table 1 monascus vinegar beverage is to the influence
of rat body weight
| Group | Number of animals (only) | Give and tried the preceding body weight (g) of thing | With high fat control group P value relatively | Give and to be tried thing body weight (g) after 60 days | With high fat control group P value relatively |
| High fat control group | 12 | 190±13 | - | ?469±18 | - |
| Low dose group | 12 | 193±11 | 0.9170 | ?472±15 | 0.7799 |
| High dose group | 12 | 191±15 | 0.7728 | ?465±21 | 0.3280 |
Visible by table 1, rat is given and is tried respectively to organize body weight before the thing and do not have significant difference.Give and to be tried thing and respectively organize body weight after 60 days and significant difference (P＞0.05) also do not occur.Show that monascus vinegar beverage does not have harmful effect to rat growthing development.
2.2 monascus vinegar beverage is to the influence of Serum TC
Table 2 monascus vinegar beverage is to the influence
of Serum TC
| Group | Number of animals (only) | ?TC(mmol/L) | ? | ? | ? |
| To before being tried thing | With high fat control group P value relatively | Tried thing after 60 days | With high fat control group P value relatively | ? | ? |
| High fat control group | 12 | ?1.81±0.17 | ?- | 3.03±0.34 | 0.00002* |
| Low dose group | 12 | ?1.78±0.19 | ?0.728015 | 2.66±0.29 | 0.063812 |
| High dose group | 12 | ?1.84±0.23 | ?0.887231 | 2.41±0.25 | 0.016378 |
* give with this group and tried to compare before the thing.
Table 2 shows, is tried relatively there was no significant difference (P＞0.05) of preceding each experimental group serum TC of thing and high fat control group.Tried thing after 60 days, relatively, serum TC significantly raises (P＜0.01) before the experiment of high fat control group and this group, shows that high TC model sets up successfully.Compare with high fat control group, the high dose group serum total cholesterol has reduced by 20% (P＜0.05).Explain that the high dose monascus vinegar beverage has the effect that reduces serum total cholesterol content.
2.3 monascus vinegar beverage is to the influence of rat blood serum TG
Table 3 monascus vinegar beverage is to the influence
of rat blood serum TG
| Group | Number of animals (only) | TG(mmol/L) | ? | ? | ? |
| To before being tried thing | With high fat control group P value relatively | Tried thing after 60 days | With high fat control group P value relatively | ? | ? |
| High fat control group | 12 | 0.74±0.11 | ?- | 1.27±0.17 | ?0.00008* |
| Low dose group | 12 | 0.75±0.16 | ?0.495102 | 1.01±0.13 | ?0.031061 |
| High dose group | 12 | 0.73±0.10 | ?0.671044 | 0.84±0.11* | ?0.000162 |
* give with this group and tried to compare before the thing.
Table 3 shows, to being tried preceding each experimental group serum TG of thing and high fat control group relatively, difference that there are no significant (P＞0.05).Tried thing after 60 days, relatively, serum TG significantly raises (P＜0.01) before the experiment of high fat control group and this group, shows that high TG model sets up successfully.Compare with high fat control group, two equal conspicuousnesses of dose groups serum triglyceride reduce.Wherein low dose group has reduced by 20% (P＜0.05); High dose group has reduced by 34% (P＜0.01) and has explained that monascus vinegar beverage has the effect that reduces serum triglyceride content.
2.4 monascus vinegar beverage is to the influence of rat blood serum HDL-C
Table 4 monascus vinegar beverage is to the influence
of rat blood serum HDL-C
| Group | Number of animals (only) | HDL-C(mmol/L) | ? |
| To before being tried thing | The P value | Tried thing after 60 days | The P value |
[0165]
| High fat control group | 12 | 1.07 ± 0.17 | - | 0.95 ± 0.13 | - |
| Low dose group | 12 | 1.03 ± 0.11 | 0.664833 | 1.12 ± 0.22 | 0.131634 |
| High dose group | 12 | 1.10 ± 0.16 | 0.871902 | 0.98 ± 0.18 | 0.317568 |
Table 4 shows, is tried thing after 60 days, with high fat control group relatively, two dose groups serum high-density LP cholesterol levels are obvious difference (P＞0.05) not.Explain that monascus vinegar beverage does not have the effect that improves the serum high-density LP cholesterol level.Approach and serum apolipoprotein that this results suggest, monascus vinegar beverage reduce serum TC, TG content have nothing to do.
3. conclusion
In sum, can know the criterion of health-care food for assisting blood fat lowering, adopt preparation embodiment 4 prepared monascus vinegar beverages to have effect for reducing blood fat according to China's " health food check and assessment technique standard " (2003 editions).
Claims (10)
1. monascus vinegar beverage that effect for reducing blood fat is arranged, it is characterized in that it can be by following method preparation: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution:
Hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more;
Wherein: the ratio of hawthorn and matrimony vine is 1: 0.5-2; The volume of institute's water is hawthorn, matrimony vine mixture 2-3 a times;
2), the preparation of monascus vinegar:
Rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter;
Wherein, the addition that adds functional red yeast rice rice fermentation is that its spit of fland content 100-200mg/L is cut down in the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage:
Thin up in hawthorn, fructus lycii extracted solution adds monascus vinegar adjustment beverage pH value then, adds honey thereafter, adds white granulated sugar again, then sterilization;
Wherein, the ratio of thin up extract is for containing two kinds of crude drugs 30-60 gram altogether according to every 1000ml beverage, and adjusted beverage pH value is pH3-4; What add honey and white granulated sugar always contains 6%-10% the most, and honey: white granulated sugar is 1: 2.5-5.
2. a kind of monascus vinegar beverage that effect for reducing blood fat is arranged according to claim 1 is characterized in that:
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine is 1: 0.5; The volume of institute's water is hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition be that its spit of fland content 100mg/L is cut down in the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 30 grams according to every 1000ml beverage; Adjusted beverage pH value is pH4; Thereafter the content that adds honey can contain honey 10g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 50g for every 1000ml.
3. a kind of monascus vinegar beverage that effect for reducing blood fat is arranged according to claim 1 is characterized in that:
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine is 1: 1; The volume of institute's water is hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition be that its spit of fland content 100mg/L is cut down in the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 40 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.5; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 60g for every 1000ml.
4. a kind of monascus vinegar beverage that effect for reducing blood fat is arranged according to claim 1 is characterized in that:
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine is 1: 1.5; The volume of institute's water is hawthorn, matrimony vine mixture 3 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition be that its spit of fland content 150mg/L is cut down in the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 50 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.2; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
5. monascus vinegar beverage that effect for reducing blood fat is arranged, it is characterized in that it can be by following method preparation: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution: hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more; Wherein: the ratio of hawthorn and matrimony vine is 1: 2; The volume of institute's water is hawthorn, matrimony vine mixture 3 times;
2), the preparation of monascus vinegar: rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter; Wherein, the addition that adds functional red yeast rice rice fermentation is that its spit of fland content 200mg/L is cut down in the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage: thin up in hawthorn, fructus lycii extracted solution, add monascus vinegar adjustment beverage pH value then, add honey thereafter, add white granulated sugar again, sterilize then;
Wherein, the ratio of thin up extract is for containing two kinds of crude drugs totally 60 grams according to every 1000ml beverage, and adjusted beverage pH value is pH 3.0; Thereafter its content that adds honey can contain honey 30g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
6. preparation method that the monascus vinegar beverage of effect for reducing blood fat is arranged, it is characterized in that it may further comprise the steps: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution:
Hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more;
Wherein: the ratio of hawthorn and matrimony vine is 1: 0.5-2; The volume of institute's water is hawthorn, matrimony vine mixture 2-3 a times;
2), the preparation of monascus vinegar:
Rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter;
Wherein, the addition that adds functional red yeast rice rice fermentation is that its spit of fland content 100-200mg/L is cut down in the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage:
Thin up in hawthorn, fructus lycii extracted solution adds monascus vinegar adjustment beverage pH value then, adds honey thereafter, adds white granulated sugar again, then sterilization;
Wherein, the ratio of thin up extract is for containing two kinds of crude drugs 30-60 gram altogether according to every 1000ml beverage, and adjusted beverage pH value is pH3-4; The total content that adds honey and white granulated sugar is 6%-10%, and honey: white granulated sugar is 1: 2.5-5.
7. a kind of preparation method that the monascus vinegar beverage of effect for reducing blood fat is arranged according to claim 6 is characterized in that:
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine is 1: 0.5; The volume of institute's water is hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition be that its spit of fland content 100mg/L is cut down in the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 30 grams according to every 1000ml beverage; Adjusted beverage pH value is pH4; Thereafter the content that adds honey can contain honey 10g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 50g for every 1000ml.
8. a kind of preparation method that the monascus vinegar beverage of effect for reducing blood fat is arranged according to claim 6 is characterized in that:
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine is 1: 1; The volume of institute's water is hawthorn, matrimony vine mixture 2 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition be that its spit of fland content 100mg/L is cut down in the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 40 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.5; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 60g for every 1000ml.
9. a kind of preparation method that the monascus vinegar beverage of effect for reducing blood fat is arranged according to claim 6 is characterized in that:
In the preparation of hawthorn, fructus lycii extracted solution, the ratio of hawthorn and matrimony vine is 1: 1.5; The volume of institute's water is hawthorn, matrimony vine mixture 3 times;
In the preparation of monascus vinegar, add functional red yeast rice rice fermentation addition be that its spit of fland content 150mg/L is cut down in the total Lip river of monascus vinegar;
In the allotment of vinegar beverage, the ratio of thin up extract can be for containing crude drug totally 50 grams according to every 1000ml beverage; Adjusted beverage pH value is pH3.2; Thereafter its content that adds honey can contain honey 20g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
10. preparation method that the monascus vinegar beverage of effect for reducing blood fat is arranged, it is characterized in that it may further comprise the steps: following consumption is a weight portion
1), the preparation of hawthorn, fructus lycii extracted solution: hawthorn is extracted with decocting with after matrimony vine mixes, with the medical filtration that extracts, will filtrate place after, filtration once more; Wherein: the ratio of hawthorn and matrimony vine is 1: 2; The volume of institute's water is hawthorn, matrimony vine mixture 3 times;
2), the preparation of monascus vinegar: rice cleans the back and pulverizes, and immersion then, steamed rice, inoculation monascus and add the fermentation of functional red yeast rice rice liquefy successively thereafter, saccharification, acetic acid bacteria fermentation, filter; Wherein, the addition that adds functional red yeast rice rice fermentation is that its spit of fland content 200mg/L is cut down in the total Lip river of monascus vinegar;
3), the allotment of vinegar beverage: thin up in hawthorn, fructus lycii extracted solution, add monascus vinegar adjustment beverage pH value then, add honey thereafter, add white granulated sugar again, sterilize then;
Wherein, the ratio of thin up extract is for containing two kinds of crude drugs totally 60 grams according to every 1000ml beverage, and adjusted beverage pH value is pH 3.0; Thereafter its content that adds honey can contain honey 30g for every 1000ml; The amount of the white granulated sugar that adds can contain granulated sugar 70g for every 1000ml.
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| CN103005379B (en) * | 2012-11-30 | 2014-02-12 | 阜阳九珍食品有限公司 | Sauce-vinegar for decreasing blood sugar and preparation method of sauce-vinegar |
| CN103740577A (en) * | 2014-02-15 | 2014-04-23 | 福建永春顺德堂食品有限公司 | Red koji vinegar and production process thereof |
| CN103865747B (en) * | 2014-03-06 | 2015-06-03 | 江南大学 | Production method of monascus vinegar rich in lovastatin |
| CN105558685B (en) * | 2015-12-15 | 2018-09-14 | 湖北佳成生物科技有限公司 | A kind of eyeshield monascus vinegar beverage and preparation method thereof |
| CN109717333A (en) * | 2019-01-10 | 2019-05-07 | 济南永扬药业有限公司 | A kind of red yeast rice Hawthorn Juice Beverages and preparation method thereof |
| CN110250380A (en) * | 2019-06-06 | 2019-09-20 | 暨南大学 | A kind of sweet potato fermented beverage and preparation method thereof with ease constipation and hypolipemic function |
| CN110250381A (en) * | 2019-06-06 | 2019-09-20 | 暨南大学 | A kind of konjaku monascus vinegar fermented beverage and preparation method thereof with ease constipation and weight losing function |
| CN112042835A (en) * | 2020-08-19 | 2020-12-08 | 天津科技大学 | Non-total nutrient special medical food with blood fat reducing function and preparation method thereof |
| CN111903971A (en) * | 2020-08-19 | 2020-11-10 | 天津科技大学 | Non-total nutrient special medical food with blood fat regulating effect and preparation method thereof |
| CN112042859A (en) * | 2020-08-19 | 2020-12-08 | 天津科技大学 | A full-nutrition special medical food with blood lipid reducing effect, and its preparation method |
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