CN101386618A - Heteroaryl benzamide derivatives for use as glk activators in the treatment of diabetes - Google Patents

Heteroaryl benzamide derivatives for use as glk activators in the treatment of diabetes Download PDF

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CN101386618A
CN101386618A CNA2008101692860A CN200810169286A CN101386618A CN 101386618 A CN101386618 A CN 101386618A CN A2008101692860 A CNA2008101692860 A CN A2008101692860A CN 200810169286 A CN200810169286 A CN 200810169286A CN 101386618 A CN101386618 A CN 101386618A
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base
oxygen base
methyl
benzamide
hydroxyl
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D·麦克克雷彻
K·G·皮克
M·J·沃林
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AstraZeneca AB
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Abstract

Compounds of formula (I) are provided, wherein R<1>, HET-1 and HET-2 are as described in the specification, and their salts and pro-drugs, are activators of glucokinase (GLK) and are thereby useful in the treatment of, for example, type 2 diabetes. Processes for preparing compounds of formula (I) are also described.

Description

In the treatment diabetes, be used as the heteroaryl benzamide derivatives of GLK activator
The application is that application number is dividing an application of 2,006 8,003 2845.2 patent application, and international application no is PCT/GB 2006/002471, and priority date is on July 9th, 2005.
The present invention relates to one group of benzoyl-amido heterocyclyl compounds, described compound can be used for treatment or the disease or the medical condition of glucokinase (GLK or GK) mediation are passed through in prevention, thereby has reduced the threshold glucose value that stimulates insulin secretion.In addition, described compound estimates it is to absorb by the increase liver glucose to reduce blood-glucose.Described compound can be used for treating type ii diabetes and obesity.The invention still further relates to the pharmaceutical composition that contains described compound, and relate to the method for described compounds for treating of using by the disease of GLK mediation.
In pancreas β cell and hepatic parenchymal cells, main plasma membrane glucose transporter is GLUT2.Under the physiology glucose concn, the speed that GLUT2 transhipment glucose passes plasma membrane does not limit the total speed of these cells to glucose uptake.The uptake rate of glucose is subjected to glucose phosphate to turn to the rate limiting of G-6-P ester (G-6-P), and this phosphorylation is by glucokinase (GLK) catalysis [1].GLK has high (6-10mM) Km to glucose, and is not subjected to the inhibition [1] of the physiological concentrations of G-6-P.GLK expresses the restriction that is subjected to a small amount of several tissue and cell type, wherein is apparent that most to be subjected to pancreas β cell and hepatocellular restriction [1].In these cells, the GLK activity limits the speed of glucose utilization, therefore the insulin secretion and the liver glycogen synthetic degree of having regulated glucose induction.These processes are quite important to the glucose homeostasis of keeping whole machine body, and dysfunction [2] all takes place in the diabetic subject.
In a kind of hypotype diabetes, promptly in the young adult form diabetes of II type (MODY-2), these diabetes are [3,4] that the GLK disappearance by function mutation causes.MODY-2 patient's hyperglycemia derives from the damaged property glucose utilization [5] in pancreas and the liver.Damaged property glucose utilization in MODY-2 patient's pancreas has caused the threshold value of glucose insulin secretion accelerating to raise.On the contrary, the activated mutant effect of rare GLK causes familial hyperinsulinemia [6,6a, 7] thereby reduced this threshold value.Except the GLK activity of observed reduction in the MODY-2 diabetes, the liver glucose kinase activity in the type ii diabetes also reduces [8].Importantly, the overall situation of GLK or liver optionally cross to be expressed, and stop or reversed the development [9-12] of diabetes phenotype in the diet of this disease and the genetic model.In addition, with the acute treatment of fructose, be by stimulating liver glucose to be used to improve glucose tolerance [13] to type ii diabetes.It is believed that this effect is that kytoplasm GLK activity increases and mediates in the liver cell of being brought out by fructose by following mechanism [13].
By regulating the activity that albumen (GLKRP) association can suppress liver GLK with GLK.The fructose-6-phosphate (F6P) that is attached on the GLKRP can make the GLK/GLKRP stable compositeization, and can make its stabilization removal by this sugared phosphoric acid of fructose-1-phosphate (F1P) displacement.F1P is that the phosphorylation by the meals fructose of fructokinase mediation produces.So the integrity of GLK/GLKRP mixture and liver GLK activity are regulated in the mode that nutrition relies on, because F6P preponderates under postabsorptive state, and F1P preponderates under state after the dining.Form contrast with liver cell, the pancreas beta cell is expressed GLK under the non-existent condition of GLKRP.So beta cell GLK activity is subjected to the adjusting of the utilizability of its substrate glucose widely.Small molecules can be directly or by making GLK/GLKRP mixture stabilization removal, activates GLK.Last compounds expectation can promote the glucose utilization in liver and the pancreas, and then a compounds is estimated optionally to work in liver.Yet expectation has the compound of above-mentioned any characteristic and all estimates to have the treatment benefit of treatment type ii diabetes, because this disease is characterised in that the glucose utilization that has all occurred damaged property in above-mentioned two kinds of tissues.
GLK, GLKRP and K ATPPassage is expressed in the hypothalamic neurone, and hypothalamus is the very important brain zone [14-18] of regulating energy balance and control ingestion of food.Verified, these neuron expressions improve a poor appetite neuropeptide and apocleisis neuropeptide [15,19,20], and inferred it is glucose sensing neurone in the hypothalamus, and the change of hypothalamus by the environment glucose concn suppresses or excited [17,19,21,22].The ability that these neurone sensation glucose levels change is damaged [23-28] in the fat model of various genetics and test-induced.Through Intraventricular (icv) infusion glucalogue (competitive inhibitor of glucokinase just), can promote the ingestion of food [29,30] of thin and weak rat.On the contrary, can suppress feed [31] through icv infusion glucose.So the small molecules activator of GLK can be by maincenter (central) the effect minimizing ingestion of food and the weight increase to GLK.So the GLK activator can be used for the treatment of to therapeutic the eating disorder except that diabetes, comprises obesity.For the treatment type ii diabetes, it will be adduction or collaborative that hypothalamic effect makes the effect of the described compound of glucose homeostasis normalizing with performance in liver and/or pancreas.So the GLK/GLKRP system can be described to (Diabesity) (act on diabetes and fat) target spot of potential " diabetes obesity ".
GLK also expresses in specific enteroendocrine cell, it is believed that it controls incretin peptide GIP (glucose-dependent insulinotropic peptide) and GLP-I (glucagon-like peptide-1) secretion of the glucose-sensitive from intestines K-cell and L-cell (32 respectively in this specific enteroendocrine cell, 33,34).Therefore, as the excretory result that can stimulate GIP and GLP-I from these enteroendocrine cells, the small molecules activator of GLK has additional beneficial effect to insulin secretion, b-cell function and survival and body weight.
In WO00/58293 and WO01/44216 (Roche), a series of benzylamino formylation compounds are described to the GLK activator.By measuring the direct effect of these compounds in the activity of GLK and the test that the generation of NADH is associated, determine that these compounds activate the mechanism of GLK, and the generation of NADH can be measured by optical property, the vitro test that detailed content can vide infra and describe.Compound of the present invention can directly activate GLK maybe can activate GLK by the interaction that suppresses GLKRP and GLK.
More GLK activator; at the WO03/095438 (phenylacetamide of replacement; Roche), WO03/055482 (methane amide and sulfone amide derivative; Novo Nordisk), WO2004/002481 (aryl carbonyl derivatives; Novo Nordisk) and WO03/080585 (the benzoyl-amido heterocycle of amino-replacement has description in Banyu).
Our International Application No. WO 03/000267 has been described one group of benzoyl-amido pyridyl carboxylic acid as enzymatic glucose kinases (GLK) activator.
Our International Application No. WO 03/015774 has been described formula (A) compound:
R wherein 3It is the heterocycle of the replacement except that the pyridyl of carboxylic acid-substituted.
In International Patent Application WO 2004/076420 (Banyu), the compound of the subclass (subset) of the compound of describing among the WO03/015774 has normally been described, wherein for example R1 is (replacement) alkyl oxide, and R 2It is (replacement) phenoxy group.
We are surprised to find, one group is selected from the compound of the subclass of the described compound of WO03/015774 usually, the GLK enzyme is had outstanding effectiveness, and have more favourable physical property, comprise for example higher water-soluble, higher permeability and/or lower plasma proteins combination.Therefore, behind oral administration, such as for example by active institute mensurations in oral glucose tolerance test (OGTTs), the described compound of equilibrated with these characteristics, expection will show the interior effectiveness of body of higher plasma free levels of drugs and Geng Jia.Therefore, expect that this group compound will provide better oral administration administration (oral exposure) to render a service with low dosage, therefore be particularly suitable for treating or preventing the disease or the medical science symptom of GLK mediation.With the compound described among other GLK activator known in the art and the WO03/015774 relatively, The compounds of this invention can also have better effectiveness and/or favourable physical properties (describing as mentioned) and/or good toxic characteristic and/or good metabolic characteristics.
Therefore, according to first aspect of the present invention, provide formula (I) compound:
Wherein:
R 1Be selected from sec.-propyl, fourth-2-base, cyclopentyl, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-hydroxyl fourth-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 1-methoxyl group fourth-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base, 1-fluorine methoxy propyl-2-base, 1,1-difluoro-methoxy third-2-base and 1-trifluoromethoxy third-2-base;
HET-1 is the heteroaryl ring that 5-or the C-of 6-unit connect, and this heteroaryl ring contains nitrogen-atoms and optional 1 or 2 other ring hetero atom that is independently selected from O, N and S in the 2-position; Described ring is chosen wantonly and be selected from R on any nitrogen-atoms 7Substituting group replace (condition is not therefore by quaternized) and/or on 1 or two suitable carbon atom, selected R independently 6Substituting group replace;
HET-2 is 5-or 6-unit hetero-aromatic ring, and this hetero-aromatic ring contains 1,2 or 3 ring hetero atom that is independently selected from O, S and N; Described ring is selected from R on the carbon atom that is fit to 2Substituting group replace, and optionally on 1 or 2 suitable carbon atom, be independently selected from R in addition 3Substituting group replace and/or on the nitrogen-atoms that is fit to (condition is not therefore by quaternized) be selected from R 10Substituting group replace;
R 2Be selected from-C (O) NR 4R 5With-SO 2NR 4R 5
R 3Be selected from methyl, trifluoromethyl and halogen;
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 4-7 unit together, and choose wantonly and contain 1 or 2 other heteroatoms (except that being connected the N atom) that is independently selected from O, N and S, wherein-CH 2-group can be chosen wantonly by-C (O)-substitute, and wherein the sulphur atom in the ring can be chosen wantonly and is oxidized to S (O) or S (O) 2Group; Described ring is chosen wantonly on the carbon atom that is fit to and is independently selected from R by 1 or 2 8Substituting group replace and/or on the nitrogen-atoms that is fit to, be selected from R 9Substituting group replace; Perhaps
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 6-10 unit's dicyclo together, optional 1 the other nitrogen-atoms (except that connecting the N atom) that contains of described heterocyclic ring, wherein-CH 2-can choose wantonly by-C (O)-replacement; Described ring is chosen wantonly on the carbon atom that is fit to and is selected from hydroxyl and R by 1 3Substituting group replace, perhaps on the nitrogen-atoms that is fit to by methyl substituted;
R 6Be independently selected from (1-4C) alkyl, halogen, hydroxyl (1-4C) alkyl, (1-4C) alkoxyl group (1-4C) alkyl, (1-4C) alkyl S (O) p (1-4C) alkyl, amino (1-4C) alkyl, (1-4C) alkylamino (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl;
R 7Be independently selected from (1-4C) alkyl, halo (1-4C) alkyl, dihalo (1-4C) alkyl, three halos ((1-4C) alkyl, hydroxyl (1-4C) alkyl, (1-4C) alkoxyl group (1-4C) alkyl, (1-4C) alkyl S (O) p (1-4C) alkyl, amino (1-4C) alkyl, (1-4C) alkylamino (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl;
R 8Be selected from hydroxyl, (1-4C) alkoxyl group, (1-4C) alkyl, aminocarboxyl, (1-4C) alkyl amino-carbonyl, two (1-4C) alkyl amino-carbonyl, (1-4C) alkylamino, two (1-4C) alkylamino, (1-4C) alkoxyl group (1-4C) alkyl, hydroxyl (1-4C) alkyl and-S (O) p (1-4C) alkyl;
R 9Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl, aminocarboxyl, (1-4C) alkyl amino-carbonyl, two (1-4C) alkyl amino-carbonyl, (1-4C) alkoxyl group (1-4C) alkyl, hydroxyl (1-4C) alkyl and-S (O) p (1-4C) alkyl;
R 10Be selected from (1-4C) alkyl, (3-6C) cycloalkyl, hydroxyl (1-4C) alkyl, (1-4C) alkoxyl group (1-4C) alkyl ,-C (O) (1-4C) alkyl, benzyl and (1-4C) alkyl sulphonyl;
P (when occurring each time independently) is 0,1 or 2;
Or its salt.
In another aspect of this invention, provide formula (I) compound of preamble definition, wherein R 1Be selected from sec.-propyl, fourth-2-base, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-hydroxyl fourth-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 1-methoxyl group fourth-2-base, 1-fluorine methoxy propyl-2-base, 1,1-difluoro-methoxy third-2-base and 1-trifluoromethoxy third-2-base;
Or its salt.
In another aspect of this invention, provide formula (I) compound of preamble definition, wherein R 1Be selected from sec.-propyl, fourth-2-base, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-hydroxyl fourth-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 1-methoxyl group fourth-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base, 1-fluorine methoxy propyl-2-base, 1,1-difluoro-methoxy third-2-base and 1-trifluoromethoxy third-2-base;
Or its salt.
In another aspect of this invention, provide formula (I) compound of preamble definition, wherein R 3It is halogen; Or its salt.
In another aspect of this invention, provide formula (I) compound of preamble definition, wherein R 7Be independently selected from (1-4C) alkyl, hydroxyl (1-4C) alkyl, (1-4C) alkoxyl group (1-4C) alkyl, (1-4C) alkyl S (O) p (1-4C) alkyl, amino (1-4C) alkyl, (1-4C) alkylamino (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl; Or its salt.
Being appreciated that definition at heterocyclic radical HET-1 and HET-2 comprises can be under the situation of substituted heteroaryl ring on the nitrogen, and such replacement cannot cause the aromaticity of charged quaternary nitrogen atoms, cancellation ring or unsettled structure.The definition that is appreciated that HET-1 and HET-2 does not comprise any O-O, O-S or S-S key.The definition that is appreciated that HET-1 and HET-2 does not comprise unsettled structure.
Be appreciated that any single carbon atom among the HET-1 only can be by a radicals R 6Replace so that the aromaticity of retaining ring.Two different carbon atoms of as many as among the HET-1 can be by R 6Group replaces, each R 6Group can be identical or different, and condition is that the structure that forms thus is stable and is aromaticity.
Be appreciated that any single carbon atom among the HET-2 only can be by a radicals R 3Replace so that the aromaticity of retaining ring.Two different carbon atoms of as many as among the HET-2 can be by R 3Group replaces, each R 3Group can be identical or different, and condition is that the structure that forms thus is stable and is aromaticity.
Be appreciated that R 8Can appear at NR 4R 5In the heterocycle that forms on arbitrary or all available carbon atoms; Each carbon atom can by 1 or 2 can be identical or different R 8Group replaces, and condition is that the structure that forms thus is stable (like this, for example, promptly do not relate to two (gem)-dihydroxyl and replace).
Be appreciated that at formula (I) compound and contain an above radicals R 5Situation under, radicals R 5Can be identical or different.
Be appreciated that at formula (I) compound and contain an above radicals R 3Situation under, R 3Can be identical or different.
Same agreement is applicable to other group and the substituting group on formula (I) compound of preamble definition.
Formula (I) compound can form the salt that belongs within the scope of the invention.Pharmacologically acceptable salt is preferred, though other salt can for example be used for the isolated or purified compound.
On the other hand, the present invention relates to formula defined above (I) compound or pharmaceutically acceptable salt thereof.
In this manual, generic term " alkyl " comprises straight chain and branched-chain alkyl.Yet, consider concrete alkyl for example " propyl group ", only refer in particular to the straight chain pattern, and the concrete branched-chain alkyl tertiary butyl for example refers in particular to the side chain pattern.For example, " (1-4C) alkyl " comprises methyl, ethyl, propyl group, sec.-propyl and the tertiary butyl.Above-mentioned agreement also is applicable to other generic terms.
For fear of causing doubt,, be meant for the 2-position of the amide nitrogen atom that connects with respect to this group about contain the group HET-1 of nitrogen in the 2-position.For example, HET-1 includes but not limited to following structure:
Figure A200810169286D00091
The suitable example of HET-1 of the heteroaryl ring that connects as 5-or the C-of 6-unit of preamble definition comprises thiazolyl, isothiazolyl, thiadiazolyl group, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidyl, oxazolyl, isoxazolyl, oxadiazole base and triazolyl.
The suitable example of HET-2 comprises thienyl, furyl, thiazolyl, isothiazolyl, thiadiazolyl group, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidyl, oxazolyl, isoxazolyl, oxadiazole base and triazolyl.The other example of HET-2 comprises that wherein to encircle nitrogen or sulphur atom oxidized but keep the aromatic heterocycle of aromaticity, for example pyridine N-oxides.The suitable in addition example of HET-2 comprises thiazolyl, pyridyl, pyrazinyl, pyridazinyl and pyrimidyl.
Define as preamble, by R 4And R 5The suitable example of the 4-7 unit ring that connected nitrogen forms together comprises morpholino, piperidyl, piperazinyl, pyrrolidyl, azetidinyl, high piperazinyl, high morpholino, high-sulfur, and (and wherein sulphur is oxidized to SO or S (O) for morpholino 2The pattern of group) and homopiperidinyl.Suitable example in addition is that thiomorpholine is for (and wherein sulphur is oxidized to SO or S (O) 2The pattern of group).
Define as preamble, by R 4And R 5The suitable example of the 6-10 unit bicyclic heterocycle that connected nitrogen forms together, be such as the saturated or part unsaturated heterocycle basic ring of dicyclo by structure shown in following (wherein dotted line is represented the tie point with the surplus portion of molecule, and wherein R represents the carbon of preamble definition or the optional substituting group of nitrogen) illustrations:
Figure A200810169286D00101
Especially such ring system is a picture
Figure A200810169286D00102
(7-azabicyclo [2.2.1] heptan-7-yl)
[2,2,1] system of such class.
In other embodiments, such ring is a picture
Figure A200810169286D00103
(2-azabicyclo [2.1.1] oneself-2-yl)
[2.1.1] system of such class.
(1-4C) example of alkyl comprises methyl, ethyl, propyl group, sec.-propyl, butyl and the tertiary butyl; (3-6C) example of cycloalkyl comprises cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl; The example of halogeno-group comprises fluorine, chlorine, bromine and iodine; The example of halo (1-4C) alkyl comprises methyl fluoride, chloromethyl, fluoro ethyl, chloroethyl, fluoropropyl and fluorine butyl; Dihalo (1-4C) alkyl comprises difluoromethyl, 1,1-difluoro second-2-base, 1,2-difluoro second-2-base, 1,1-two chloroethenes-2-base, 1,2-two chloroethenes-2-base and 1,1-difluoro third-3-base; (example of (1-4C) alkyl comprises trifluoromethyl and 1,1 to three halos, 1-trifluoro second-2-base; The example of hydroxyl (1-4C) alkyl comprises hydroxymethyl, 1-hydroxyethyl, 2-hydroxyethyl, 2-hydroxypropyl, 3-hydroxypropyl, 1-hydroxyl sec.-propyl and 4-hydroxybutyl; (1-4C) example of alkoxyl group (1-4C) alkyl comprises methoxymethyl, ethoxyl methyl, tert.-butoxy methyl, 2-methoxy ethyl, 2-ethoxyethyl group, methoxy-propyl, 2-methoxy-propyl and methoxyl group butyl; (1-4C) example of alkoxyl group comprises methoxyl group, oxyethyl group, propoxy-, isopropoxy, butoxy and tert.-butoxy; (1-4C) example of alkyl S (O) p (1-4C) alkyl (wherein p is 0,1 or 2) comprises methylsulfinyl methyl, ethyl sulfinyl methyl, ethyl sulfinyl ethyl, methylsulfinyl propyl group, methylsulfinyl butyl, sulfonyloxy methyl ylmethyl, ethylsulfonyl methyl, ethylsulfonyl ethyl, methyl sulphonyl propyl group, methyl sulphonyl butyl, methylthiomethyl, ethylmercapto group methyl, ethylmercapto group ethyl, methylthio group propyl group and methylthio group butyl; (1-4C) example of alkyl sulphonyl comprises methyl sulphonyl, ethylsulfonyl, sulfonyl propyl base, sec.-propyl alkylsulfonyl and tertiary butyl alkylsulfonyl; The example of-S (O) p (1-4C) alkyl comprises (1-4C) alkyl sulphonyl, methylsulfinyl, ethyl sulfinyl, propyl group sulfinyl, sec.-propyl sulfinyl, tertiary butyl sulfinyl, methylthio group, ethylmercapto group, rosickyite base, iprotiazem base and uncle's butylthio; The example of amino (1-4C) alkyl comprises amino methyl, amino-ethyl, 2-aminopropyl, 3-aminopropyl, the amino sec.-propyl of 1-and the amino butyl of 4-; (1-4C) example of alkylamino (1-4C) alkyl comprises (N-methyl) amino methyl, (N-ethyl) amino methyl, 1-((N-methyl) amino) ethyl, 2-((N-methyl) amino) ethyl, (N-ethyl) amino-ethyl, (N-methyl) aminopropyl and 4-((N-methyl) amino) butyl; The example of two (1-4C) alkylamino (1-4C) alkyl comprises dimethylaminomethyl, methyl (ethyl) amino methyl, methyl (ethyl) amino-ethyl, (N, the N-diethyl) amino-ethyl, (N, the N-dimethyl) aminopropyl and (N, N-dimethyl) amino butyl;-C (O) (1-4C) alkyl and (1-4C) example of alkyl-carbonyl comprise methyl carbonyl, ethyl carbonyl, propyl group carbonyl and tertiary butyl carbonyl; (1-4C) example of alkylamino comprises methylamino, ethylamino, propyl group amino, sec.-propyl amino, butyl amino and tertiary butyl amino; The example of two (1-4C) alkylamino comprises dimethylamino, diethylamino, N-methyl-N-ethylamino, dipropyl amino, N-sec.-propyl-N-methylamino and dibutylamino; (1-4C) example of alkyl amino-carbonyl comprises methylamino carbonyl, ethylamino carbonyl, propyl group aminocarboxyl, sec.-propyl aminocarboxyl, butyl aminocarboxyl and tertiary butyl aminocarboxyl; The example of two (1-4C) alkyl amino-carbonyl comprises dimethylamino carbonyl, diethylamino carbonyl, N-methyl-N-ethylamino carbonyl, dipropyl aminocarboxyl, N-sec.-propyl-N-amino-carbonyl and dibutylamino carbonyl.
Be appreciated that, reason owing to one or more unsymmetrical carbons, hereinbefore in the scope of Ding Yi formula (I) compound, may have optical activity or racemic modification form, above definition the present invention includes any this class optical activity or the raceme formalization compound that has direct stimulation GLK or suppress the GLK/GLKRP interaction property.Can adopt the known technology of organic chemistry filed to come the synthesis of optically active form, for example can be synthetic by optically active starting raw material, perhaps obtain by the resolution of racemic form.Therefore be appreciated that some compound exists with tautomeric form, the present invention also relates to activate any and all tautomeric forms of the The compounds of this invention of GLK.
Be further appreciated that some formula (I) compound and salt thereof can be with the form of solvate, non-solvent compound for example the form of hydrate exist.Be appreciated that and the present invention includes all such solvate forms that activate GLK.
On the other hand, the present invention relates to formula (I) compound or its prodrug of preamble definition.The suitable example of the prodrug of formula (I) compound is the interior hydrolyzable ester of the body of formula (I) compound.Therefore, on the other hand, the present invention relates to hydrolyzable ester in formula defined above (I) compound or its body.
In one embodiment, the invention provides formula (I) compound.In another embodiment, the invention provides the pharmacologically acceptable salt of formula (I) compound, in another embodiment, the present invention also provides the body of formula (I) compound interior hydrolyzable ester, and the present invention further provides in another embodiment, the pharmacologically acceptable salt of the interior hydrolyzable ester of body of formula (I) compound.
Compound of the present invention can adopt the form administration of prodrug.Prodrug is a degradable and generate the bioprecursor of The compounds of this invention or pharmacy can be accepted compound (for example hydrolyzable ester in the ester of The compounds of this invention or the acid amides, particularly body) in vivo.The various forms of prodrug is known in the art.The example of described prodrug derivant, referring to::
A) Design of Prodrugs, H.Bundgaard edits, (Elsevier, 1985) and Methods inEnzymology, Vol. 42, p.309-396, people such as K.Widder edit (Academic Press, 1985);
B) A Textbook of Drug Design and Development, Krogsgaard-Larsen edits;
C) H.Bundgaard, the 5th chapter " Design and Application of Prodrugs ", H.Bundgaard edits, 113-191 page or leaf (1991);
d) H.Bundgaard，Advanced?Drug?Delivery?Reviews， 8，1-38(1992)；
E) people such as H.Bundgaard, Journal of Pharmaceutical Sciences, 77, 285 (1988); And f) N.Kakeya waits the people, Chem Pharm Bull, 32, 692 (1984).
The content of above-mentioned citing document is incorporated herein this paper as a reference.
The example of prodrug is as follows.The interior hydrolyzable ester of body that contains the The compounds of this invention of carboxyl or hydroxyl is that for example hydrolysis produces parent acid or pure pharmaceutically acceptable ester in people or animal body.The suitable pharmaceutically acceptable ester of carboxyl comprises C 1-C 6The alkoxy methyl ester is methoxyl methyl for example, C 1-C 6Alkyloyl oxygen methyl ester is the pivalyl yloxymethyl for example, phthalidyl ester, C 3-C 8Cycloalkyloxy carbonyl oxygen base C 1-C 6Alkyl ester is 1-cyclohexyl carbonyl oxygen base ethyl for example; 1,3-dioxole-2-ketone group methyl ester, 5-methyl isophthalic acid for example, 3-dioxole-2-ketone group methyl; (1-6C) alkoxyl group carbonyl oxygen base ethyl ester.
Contain hydrolyzable ester in the body of The compounds of this invention of hydroxyl, comprise inorganic ester for example phosphoric acid ester (comprising amino phosphono cyclic ester) and alpha-acyloxy alkyl oxide and related compound, it is ester hydrolysis and obtain the result of the hydroxyl of parent in vivo.The example of alpha-acyloxy alkyl oxide comprises acetoxyl group methoxyl group and 2,2-dimethyl propylene acyloxy-methoxyl group.Concerning hydroxyl, the selection of hydrolyzable ester comprises benzoyl and phenyl acetyl, alkoxy carbonyl (acquisition alkyl carbonate), dialkyl amido formyl radical and N-(dialkyl amido ethyl)-N-alkyl-carbamoyl (acquisition carbamate), dialkyl amido ethanoyl and the carboxyl ethanoyl of alkyloyl, benzoyl, phenyl acetyl and replacement in the organizer.
The suitable pharmacologically acceptable salt of The compounds of this invention is, for example, enough acid salt of Jian Xing The compounds of this invention, for example, with the acid salt that forms of hydrochloric acid, Hydrogen bromide, sulfuric acid, phosphoric acid, trifluoroacetic acid, citric acid or toxilic acid for example of inorganic or organic acid for example.In addition, the suitable pharmacologically acceptable salt of enough tart benzoxazinone derivatives of the present invention is an an alkali metal salt, for example sodium or sylvite, alkaline earth salt, for example calcium or magnesium salts, ammonium salt or with provide the physiology salt that acceptable cationic organic bases forms, the salt that forms with methylamine, dimethyl amine, Trimethylamine, piperidines, morpholine or three-(2-hydroxyethyl) amine for example.
In another aspect of this invention, providing it is formula (I) compound of formula (IA) compound or pharmaceutically acceptable salt thereof:
Figure A200810169286D00131
X wherein 1, X 2And X 3Be selected from CH, N, S and O independently of one another; X 4Be non-existent (form 5-unit ring) or be selected from CH, N, O and S;
Condition is X 1, X 2, X 3And X 4In at least one is CH, and condition is not have O-O, O-S or S-S key in this ring;
R 3If, exist, be selected from methyl, trifluoromethyl and halogeno-group;
R 1, R 2With HET-1 as for formula (I) compound definition.
Be appreciated that and contain X 1-X 4The inner dashed circle of ring (be HET-2 ring) represent that described ring is an aromaticity, though X is depended in the accurate number and the position of two keys 1-X 4Character.
Whether this paper mentions formula (I) compound, no matter offer some clarification on, and generally should be understood to be equally applicable to formula (IA) compound, unless context is pointed out in addition.
Formula (I) and (IA) particular instance of compound comprise formula (IB), (IC) and/or (ID) compound:
Figure A200810169286D00141
R wherein 1, R 2With HET-1 as defined for formula (I) compound.
The preferred value of each variable group is as follows.Such value can be used with any value, definition, claim, aspect or the embodiment of preamble or hereinafter definition in appropriate circumstances.Especially, each described value can be with the restriction of doing formula (I) or the most extensive definition (IA).And, each following value can with one or more other following values associatings, be used for aspect preamble or hereinafter any, restraint-type (I) or the most extensive definition (IA), perhaps restraint-type (I) or the less definition of any scope (IA).In appropriate circumstances, each down train value can also be used for restraint-type (IB), (IC) and/or (ID) in any definition.
(1) R 1Be inferior formula X:
Figure A200810169286D00151
R wherein xBe selected from methyl, ethyl, trifluoromethyl, ethynyl, hydroxymethyl, hydroxyethyl, methoxymethyl, fluorine methoxymethyl, difluoro-methoxy methyl and trifluoromethoxy methyl; Preferred R xBe selected from methyl, ethyl, trifluoromethyl, ethynyl, hydroxymethyl, hydroxyethyl, methoxymethyl, fluorine methoxymethyl and difluoro-methoxy methyl
(2) R 1Be inferior formula Y:
Figure A200810169286D00152
R wherein yBe selected from hydroxymethyl and methoxymethyl
(3) R 1Be 1-hydroxyl third-2-base and configuration preferably (S), i.e. R 1-O-is:
Figure A200810169286D00153
(4) R 1Be 1-methoxy propyl-2-base and configuration preferably (S), i.e. R 1-O-is
Figure A200810169286D00154
(5) R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, hydroxyl fourth-3-base and 1-methoxy propyl-2-base
(6) R 1Be 1,1,1-trifluoropropyl-2-base, 1-fluorine methoxy propyl-2-base, 1,1-difluoro-methoxy third-2-base or 1-trifluoromethoxy third-2-base
(7) R 1Be 1-fluorine methoxy propyl-2-base, 1,1-difluoro-methoxy third-2-base or 1-trifluoromethoxy third-2-base, particularly 1-fluorine methoxy propyl-2-base or 1,1-difluoro-methoxy third-2-base
(8) R 1Be 1,1-difluoro-methoxy third-2-base particularly has stereochemistry:
Figure A200810169286D00161
(9) R 1Be tetrahydrofuran base or THP trtrahydropyranyl
(10) R 1Be the tetrahydrofuran base of (S) configuration, that is:
(11) R 1Be the tetrahydrofuran base of (R) configuration, that is:
Figure A200810169286D00163
(12) R 1Be the 4-THP trtrahydropyranyl:
Figure A200810169286D00164
(13) R 1Be that 2-hydroxyl-Ding-3-base and this configuration preferably make R 1-O-is:
(14) R 1Be 1-hydroxyl fourth-2-base or 1-methoxyl group fourth-2-base
(15) R 1Be selected from sec.-propyl, fourth-2-base, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 1-fluorine methoxy propyl-2-base, 1,1-difluoro-methoxy third-2-base and 1-trifluoromethoxy third-2-base
(16) R 1Be selected from 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base and 2-methoxyl group fourth-1-base
(17) R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1,1-difluoro-methoxy third-2-base, tetrahydrofuran base, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base
(18) R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1,1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base
(19) R 1Be selected from tetrahydrofuran base, 1-difluoro-methoxy third-2-base, 1,3-difluoro third-2-base and 2-hydroxyl fourth-3-base
(20) R 1Be selected from sec.-propyl, tetrahydrofuran base, 1-hydroxyl third-2-base and 1-methoxy propyl-2-base
(21) R 1Be selected from 1-hydroxyl third-2-base and 1-methoxy propyl-2-base, for example (2S)-1-hydroxyl third-2-base with (2S)-1-methoxy propyl-2-base
(22) R 1Be selected from sec.-propyl, fourth-2-base, cyclopentyl, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-hydroxyl fourth-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 1-methoxyl group fourth-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base, 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base
(23) R 1Be selected from sec.-propyl, fourth-2-base, cyclopentyl, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-hydroxyl fourth-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 1-methoxyl group fourth-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base and 1,1-difluoro-methoxy third-2-base
(24) HET-1 is a 5-unit heteroaryl ring
(25) HET-1 is a 6-unit heteroaryl ring
(26) HET-1 is independently selected from R by 1 or 2 6Substituting group replace
(27) HET-1 is selected from R by one 6Substituting group replace
(28) HET-1 is selected from R by one 7Substituting group replace
(29) HET-1 is unsubstituted
(30) HET-1 is selected from thiazolyl, isothiazolyl, thiadiazolyl group, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidyl, oxazolyl, isoxazolyl, oxadiazole base and triazolyl
(31) HET-1 is selected from thiazolyl, isothiazolyl, thiadiazolyl group, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl with the oxadiazole base
(32) HET-1 is selected from pyridyl, pyrazinyl, pyridazinyl and pyrimidyl
(33) HET-1 is selected from thiazolyl, pyrazolyl He oxazolyl
(34) HET-1 is selected from thiadiazolyl group with the oxadiazole base
(35) HET-1 is selected from 1,3,4-thiadiazolyl group and 1,3,4-oxadiazole base
(36) HET-1 is selected from 1,2,4-oxadiazole base and 1,2,4-oxadiazole base
(37) HET-1 is a pyrazolyl, especially N-methylpyrazole base
(38) HET-1 is a pyrazinyl
(39) HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl
(40) HET-1 is selected from optional thiazolyl, pyrazolyl and the thiadiazolyl group that is replaced by (1-4C) alkyl
(41) HET-1 is a pyrazolyl, especially N-methylpyrazole base
(42) HET-1 is that pyrazolyl (optional by ethyl, sec.-propyl or 1 or 2 methyl substituted), thiazolyl (optional by methyl substituted), pyrazinyl (optional by methyl substituted), pyridyl (are optionally replaced) ， isoxazolyl (optional by methyl substituted) and thiadiazolyl group (choosing wantonly by methyl substituted) by fluorine
(43) HET-1 is that pyrazolyl (optional by ethyl, sec.-propyl, difluoromethyl or 1 or 2 methyl substituted), thiazolyl (optional by methyl substituted), pyrazinyl (optional by methyl substituted), pyridyl (are optionally replaced), isoxazolyl (optional by methyl substituted) and thiadiazolyl group (choosing wantonly by methyl substituted) by fluorine
(44) HET-1 is selected from pyrazinyl (optional by methyl substituted), pyrazolyl (choosing wantonly on carbon by methyl substituted), methyl thiazolium di azoly (especially 1,2,4-thiadiazoles-5-base, more preferably 3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl), thiazolyl (optional by methyl substituted), pyridyl (are optionally replaced) with isoxazolyl by fluorine
(45) HET-1 is the N-difluoromethylpyrazocarboxylates
(46) HET-1 is 5-methylpyrazine-2-base
(47) HET-1 is that pyrazolyl (optional by ethyl, sec.-propyl, difluoromethyl or 1 or 2 methyl substituted), thiazolyl (optional by methyl substituted), pyrazinyl (optional by methyl substituted), pyridyl (are optionally replaced) ， isoxazolyl (optional by methyl substituted) and thiadiazolyl group (choosing wantonly by methyl substituted) by fluorine; With
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1,1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base; If HET-1 is unsubstituted pyrazolyl on nitrogen (being the NH-pyrazolyl), R so 1Especially be selected from 1-methoxy propyl-2-base, sec.-propyl and tetrahydrofuran base
(48) HET-1 is that pyrazolyl (choosing wantonly on carbon by methyl substituted), thiazolyl (optional by methyl substituted), pyrazinyl (optional by methyl substituted), pyridyl (are optionally replaced), isoxazolyl and methyl thiazolium di azoly (3-methyl isophthalic acid especially by fluorine, 2,4-thiadiazoles-5-yl);
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1,1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base; If HET-1 is a pyrazolyl, R1 especially is selected from 1-methoxy propyl-2-base, sec.-propyl and tetrahydrofuran base so
(49) HET-1 is methylpyrazine base and R 1Be selected from 1-hydroxyl third-2-base and 1-methoxy propyl-2-base, for example (2S)-1-hydroxyl third-2-base with (2S)-1-methoxy propyl-2-base
(50) HET-1 is pyrazolyl and R 1Be selected from sec.-propyl, tetrahydrofuran base and 1-methoxy propyl-2-base, for example (2S)-1-methoxy propyl-2-base
(51) R 6Be selected from (1-4C) alkyl, halogen, hydroxyl (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl
(52) R 6Be selected from methyl, ethyl, bromine, chlorine, fluorine, hydroxymethyl, methoxymethyl, amino methyl, N-methylamino methyl, dimethylaminomethyl
(53) R 6Be selected from (1-4C) alkyl, halogen, hydroxyl (1-4C) alkyl, (1-4C) alkoxyl group (1-4C) alkyl, (1-4C) alkyl S (O) p (1-4C) alkyl, amino (1-4C) alkyl, (1-4C) alkylamino (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl
(54) R 6Be selected from methyl, ethyl, bromine, chlorine, fluorine, hydroxymethyl and methoxymethyl
(55) R 6Be selected from methyl, ethyl, chlorine and fluorine
(56) R 6Be methyl or fluorine, preferable methyl
(57) R 6Be selected from methyl, ethyl, bromine, chlorine, fluorine, amino methyl, N-methylamino methyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
(58) R 6Be selected from methyl, ethyl, amino methyl, N-methylamino methyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
(59) R 6Be selected from methyl, ethyl, sec.-propyl and methoxymethyl
(60) if there are 2 substituent R 6, both all are selected from methyl, ethyl, bromine, chlorine and fluorine so; Preferably both are all methyl
(61) R 6Be selected from (1-4C) alkyl S (O) p (1-4C) alkyl, (1-4C) alkylamino (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl
(62) R 7Be selected from (1-4C) alkyl, hydroxyl (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl
(63) R 7Be selected from methyl, ethyl, hydroxymethyl, methoxymethyl, amino methyl, N-methylamino methyl, dimethylaminomethyl
(64) R 7Be selected from (1-4C) alkyl, hydroxyl (1-4C) alkyl, (1-4C) alkoxyl group (1-4C) alkyl, (1-4C) alkyl S (O) p (1-4C) alkyl, amino (1-4C) alkyl, (1-4C) alkylamino (1-4C) alkyl and two (1-4C) alkylamino (1-4C) alkyl
(65) R 7Be selected from methyl, ethyl, amino methyl, N-methylamino methyl and dimethylaminomethyl
(66) R 7Be selected from methyl, ethyl, hydroxymethyl and methoxymethyl
(67) R 7Be selected from methyl, sec.-propyl and ethyl
(68) R 7Be selected from methyl, sec.-propyl, difluoromethyl and ethyl
(69) R 7Be selected from sec.-propyl and difluoromethyl, especially difluoromethyl
(70) R 7Be selected from methyl and ethyl
(71) R 7It is methyl
(72) R 7Be selected from methyl, ethyl, amino methyl, N-methylamino methyl, dimethylaminomethyl, hydroxymethyl and methoxymethyl
(73) R 7Be selected from methyl, ethyl, sec.-propyl and methoxymethyl
(74) if R 7Be selected from halo (1-4C) alkyl, dihalo (1-4C) alkyl and three halos (1-4C) alkyl, each halogeno-group is selected from chlorine and fluorine so, and fluorine especially
(75) if R 7Be selected from halo (1-4C) alkyl, dihalo (1-4C) alkyl and three halos (1-4C) alkyl, R so 7Especially be selected from methyl fluoride, two fluoro ethyls, difluoromethyl and trifluoromethyl
(76) HET-2 is a 5-unit ring
(77) HET-2 is a 6-unit ring
(78) HET-2 is selected from thienyl, furyl, thiazolyl, isothiazolyl, thiadiazolyl group, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidyl, oxazolyl, isoxazolyl with the oxadiazole base
(79) HET-2 is selected from thienyl, furyl, thiadiazolyl group, pyridyl, pyrazinyl, pyridazinyl, pyrazolyl, imidazolyl, pyrimidyl He oxadiazole base
(80) HET-2 is selected from pyridyl, pyrazinyl, thiazolyl and thienyl
(81) HET-2 is selected from pyridyl, pyrazinyl and thiazolyl
(82) HET-2 is selected from pyridyl, pyrazinyl, pyridazinyl and thiazolyl
(83) HET-2 is selected from pyridyl and pyrazinyl
(84) HET-2 is a pyrazinyl
(85) HET-2 is selected from R 3Substituting group replace
(86) HET-2 has one and is selected from R 10The nitrogen substituting group
(87) R 3Be chlorine or fluorine
(88) R 3Be chlorine
(89) R 3It is fluorine
(90) R 3Be chlorine or methyl
(91) R 3Be fluorine, chlorine or methyl
(92) R 2Be-C (O) NR 4R 5
(93) R 2Be-SO 2NR 4R 5
(94) R 4And R 5Connected nitrogen-atoms forms 4 yuan of rings together
(95) R 4And R 5Connected nitrogen-atoms forms 5 yuan of rings together
(96) R 4And R 5Connected nitrogen-atoms forms 6 yuan of rings together
(97) R 4And R 5Connected nitrogen-atoms forms 7 yuan of rings together
(98) R 4And R 5Connected nitrogen-atoms forms saturated ring fully together
(99) R 4And R 5Connected nitrogen-atoms forms the ring that is selected from morpholino, piperidyl, piperazinyl, pyrrolidyl and azetidinyl together
(100) R 4And R 5Connected nitrogen-atoms forms the ring that is selected from pyrrolidyl, morpholino and azetidinyl together
(101) R 4And R 5Connected nitrogen-atoms forms the ring that is selected from 7-azabicyclo [2.2.1] heptan-7-base, pyrrolidyl, morpholino and azetidinyl together
(102) R 4And R 5Connected nitrogen-atoms forms the azetidine basic ring together
(103) R 4And R 5Connected nitrogen-atoms forms azetidinyl or tetramethyleneimine basic ring together
(104) R 4And R 5Connected nitrogen-atoms forms unsubstituted ring together
(105) R 4And R 5Connected nitrogen-atoms forms together and is substituted basic R 8Or be substituted basic R 9Monobasic ring
(106) R 4And R 5Connected nitrogen-atoms forms the saturated or unsaturated ring of part of 6-10 unit's dicyclo together
(107) R 8Be selected from hydroxyl, (1-4C) alkoxyl group, (1-4C) alkyl
(108) R 8Be selected from hydroxyl, methoxyl group and methyl
(109) R 9Be selected from (1-4C) alkyl and-(1-4C) alkyl of C (O)
(110) R 2Be azetidinyl carbonyl or pyrrolidyl carbonyl, preferred azetidinyl carbonyl
(109) R 10It is (1-4C) alkyl
(111) R 10It is (3-6C) cycloalkyl
(112) R 10Be hydroxyl (1-4C) alkyl or (1-4C) alkoxyl group (1-4C) alkyl
(113) R 10It is-C (O) (1-4C) alkyl
(114) R 10It is benzyl
(115) R 10It is (1-4C) alkyl sulphonyl
(116) R 10Be (1-4C) alkyl or benzyl
According to another feature of the present invention, provide the following preferred group of The compounds of this invention:
In one aspect of the invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, fourth-2-base, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is the heteroaryl ring that the C-of 5-or 6-unit connects, and this heteroaryl ring contains nitrogen-atoms in the 2-position, and optionally contains 1 or 2 other heteroatoms that is independently selected from O, N and S; Described ring is chosen wantonly (condition is not therefore by quaternized) on any nitrogen-atoms and is replaced by methyl or ethyl and/or replaced by methyl or ethyl on 1 or 2 suitable carbon atom;
HET-2 is 5-or 6-unit heteroaryl ring, and this heteroaryl ring contains 1,2 or 3 ring hetero atom that is independently selected from O, S and N; Described ring is selected from R2 on suitable carbon atom substituting group replaces, and optional in addition the substituting group that is independently selected from R3 on 1 or 2 suitable carbon atom replace and/or on suitable nitrogen-atoms (condition is not therefore by quaternized) quilt be selected from R 10Substituting group replace;
R 2Be selected from-C (O) NR 4R 5With-SO 2NR 4R 5
R 3Be selected from halogeno-group;
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 4-7 unit together, and choose wantonly and contain 1 or 2 other heteroatoms (except that the N atom that is connected) that is independently selected from O, N and S, wherein-CH 2-group can be chosen wantonly by-C (O)-substitute, and wherein the sulphur atom in this ring can be chosen wantonly and is oxidized to S (O) or S (O) 2Group; Described ring is chosen wantonly on suitable carbon atom and is independently selected from R by 1 or 2 8Substituting group replace and/or on suitable nitrogen-atoms, be selected from R 9Substituting group replace;
R 8Be selected from hydroxyl, (1-4C) alkoxyl group and (1-4C) alkyl;
R 9Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl and-S (O) p (1-4C) alkyl;
R 10Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl, benzyl and (1-4C) alkyl sulphonyl;
P (when occurring each time independently) is 0,1 or 2.
In one aspect of the invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, fourth-2-base, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base, 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is the heteroaryl ring that the C-of 5-or 6-unit connects, and this heteroaryl ring contains nitrogen-atoms in the 2-position, and optionally contains 1 or 2 other heteroatoms that is independently selected from O, N and S; Described ring is chosen wantonly (condition is that it is not therefore by quaternized) on any nitrogen-atoms and is replaced by methyl or ethyl and/or replaced by methyl or ethyl on 1 or 2 suitable carbon atom;
HET-2 is 5-or 6-unit heteroaryl ring, and this heteroaryl ring contains 1,2 or 3 ring hetero atom that is independently selected from O, S and N; Described ring is selected from R on suitable carbon atom 2Substituting group replace, and optionally on 1 or 2 suitable carbon atom, be independently selected from R in addition 3Substituting group replace and/or on suitable nitrogen-atoms (condition is that it is not therefore by quaternized) be selected from R 10Substituting group replace;
R 2Be selected from-C (O) NR 4R 5With-SO 2NR 4R 5
R 3Be selected from halogeno-group;
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 4-7 unit together, and choose wantonly and contain 1 or 2 other heteroatoms (except that the N atom that is connected) that is independently selected from O, N and S, wherein-CH 2-group can be chosen wantonly by-C (O)-substitute, and wherein the sulphur atom in this ring can be chosen wantonly and is oxidized to S (O) or S (O) 2Group; Described ring is chosen wantonly on suitable carbon atom and is independently selected from R by 1 or 2 8Substituting group replace and/or on suitable nitrogen-atoms, be selected from R 9Substituting group replace;
R 8Be selected from hydroxyl, (1-4C) alkoxyl group and (1-4C) alkyl;
R 9Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl and-S (O) p (1-4C) alkyl;
R 10Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl, benzyl and (1-4C) alkyl sulphonyl;
P (when occurring each time independently) is 0,1 or 2.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, fourth-2-base, cyclopentyl, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base, 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is the heteroaryl ring that the C-of 5-or 6-unit connects, and this heteroaryl ring contains nitrogen-atoms in the 2-position, and optionally contains 1 or 2 other ring hetero atom that is independently selected from O, N and S; Described ring is chosen wantonly (condition is that it is not therefore by quaternized) on any nitrogen-atoms and is replaced by methyl, ethyl or sec.-propyl and/or replaced by methyl, ethyl or fluorine on 1 or 2 suitable carbon atom;
HET-2 is 5-or 6-unit heteroaryl ring, and this heteroaryl ring contains 1,2 or 3 ring hetero atom that is independently selected from O, S and N; Described ring is selected from R on suitable carbon atom 2Substituting group replace, and optionally on 1 or 2 suitable carbon atom, be independently selected from R in addition 3Substituting group replace and/or on suitable nitrogen-atoms (condition is that it is not therefore by quaternized) be selected from R 10Substituting group replace;
R 2Be selected from-C (O) NR 4R 5With-SO 2NR 4R 5
R 3Be selected from halogeno-group;
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 4-7 unit together, and choose wantonly and contain 1 or 2 other heteroatoms (except that the N atom that is connected) that is independently selected from O, N and S, wherein-CH 2-group can be chosen wantonly by-C (O)-substitute, and wherein the sulphur atom in this ring can be chosen wantonly and is oxidized to S (O) or S (O) 2Group; Described ring is chosen wantonly on suitable carbon atom and is independently selected from R by 1 or 2 8Substituting group replace and/or on suitable nitrogen-atoms, be selected from R 9Substituting group replace;
R 8Be selected from hydroxyl, (1-4C) alkoxyl group and (1-4C) alkyl;
R 9Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl and-S (O) p (1-4C) alkyl;
R 10Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl, benzyl and (1-4C) alkyl sulphonyl;
P (when occurring each time independently) is 0,1 or 2.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, fourth-2-base, cyclopentyl, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base, 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is the heteroaryl ring that the C-of 5-or 6-unit connects, and this heteroaryl ring contains nitrogen-atoms in the 2-position, and optionally contains 1 or 2 other ring hetero atom that is independently selected from O, N and S; Described ring is chosen wantonly (condition is that it is not therefore by quaternized) on any nitrogen-atoms and is replaced by methyl, ethyl or sec.-propyl and/or replaced by methyl, ethyl or fluorine on 1 or 2 suitable carbon atom;
HET-2 is 5-or 6-unit heteroaryl ring, and this heteroaryl ring contains 1,2 or 3 ring hetero atom that is independently selected from O, S and N; Described ring is selected from R on suitable carbon atom 2Substituting group replace, and optionally on 1 or 2 suitable carbon atom, be independently selected from R in addition 3Substituting group replace and/or on suitable nitrogen-atoms (condition is that it is not therefore by quaternized) be selected from R 10Substituting group replace;
R 2Be selected from-C (O) NR 4R 5With-SO 2NR 4R 5
R 3Be selected from methyl and halogeno-group;
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 4-7 unit together, and choose wantonly and contain 1 or 2 other heteroatoms (except that the N atom that is connected) that is independently selected from O, N and S, wherein-CH 2-group can be chosen wantonly by-C (O)-substitute, and wherein the sulphur atom in this ring can be chosen wantonly and is oxidized to S (O) or S (O) 2Group; Described ring is chosen wantonly on suitable carbon atom and is independently selected from R by 1 or 2 8Substituting group replace and/or on suitable nitrogen-atoms, be selected from R 9Substituting group replace;
R 8Be selected from hydroxyl, (1-4C) alkoxyl group and (1-4C) alkyl;
R 9Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl and-S (O) p (1-4C) alkyl;
R 10Be selected from (1-4C) alkyl ,-C (O) (1-4C) alkyl, benzyl and (1-4C) alkyl sulphonyl;
P (when occurring each time independently) is 0,1 or 2.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, fourth-2-base, cyclopentyl, 1,1,1-trifluoropropyl-2-base, 1,3-difluoro third-2-base, fourth-1-alkynes-3-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-methoxy propyl-2-base, 2-hydroxyl third-1-base, 2-methoxy propyl-1-base, 2-hydroxyl fourth-1-base, 2-methoxyl group fourth-1-base, 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is the heteroaryl ring that the C-of 5-or 6-unit connects, and this heteroaryl ring contains nitrogen-atoms in the 2-position, and optionally contains 1 or 2 other ring hetero atom that is independently selected from O, N and S; Described ring is chosen wantonly (condition is that it is not therefore by quaternized) on any nitrogen-atoms and is replaced by methyl, ethyl or sec.-propyl and/or replaced by methyl, ethyl or fluorine on 1 or 2 suitable carbon atom;
HET-2 is 5-or 6-unit heteroaryl ring, and this heteroaryl ring contains 1,2 or 3 ring hetero atom that is independently selected from O, S and N; Described ring is selected from R on suitable carbon atom 2Substituting group replace, and optionally on 1 or 2 suitable carbon atom, be independently selected from R in addition 3Substituting group replace;
R 2Be selected from-C (O) NR 4R 5With-SO 2NR 4R 5
R 3Be selected from methyl and halogeno-group;
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 4-7 unit together, and choose wantonly and contain 1 or 2 other heteroatoms (except that the N atom that is connected) that is independently selected from O, N and S, wherein-CH 2-group can be chosen wantonly by-C (O)-substitute, and wherein the sulphur atom in this ring can be chosen wantonly and is oxidized to S (O) or S (O) 2Group; Perhaps
R 4And R 5Connected nitrogen-atoms forms the saturated or part unsaturated heterocycle basic ring of 6-10 unit's dicyclo together, optional 1 the other nitrogen-atoms (except that the N atom that connects) that contains, wherein-CH 2-group can be chosen wantonly by-C (O)-substitute.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, fourth-2-base, 1,3-difluoro third-2-base, 1-hydroxyl third-2-base and 1-methoxy propyl-2-base and tetrahydrofuran base;
HET-1 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
HET-2 is 5-or the 6-unit heteroaryl ring that replaces, and defines as preamble;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
HET-2 is 5-or the 6-unit heteroaryl ring that replaces, and defines as preamble;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
HET-2 is 5-or the 6-unit heteroaryl ring that replaces, and defines as preamble;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl or tetramethyleneimine basic ring together.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, 1-hydroxyl third-2-base and 1-methoxy propyl-2-base,
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl, wherein R 1Choose wantonly on carbon or nitrogen and replaced by methyl or ethyl;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, thiazolyl and the thienyl that replace;
R 3It is fluorine or chlorine;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl, wherein R 1Optional by methyl, sec.-propyl or ethyl replacement;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, thiazolyl and the pyrimidyl that replace;
R 3It is fluorine or chlorine;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group, pyridyl, isoxazolyl and pyrazinyl, wherein R 1Choose wantonly by methyl, sec.-propyl or ethyl replacement and/or (on carbon atom) and replaced by fluorine;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, pyridazinyl, thiazolyl and the pyrimidyl that replace;
R 3It is fluorine or chlorine;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl or tetramethyleneimine basic ring together.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein: R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group, pyridyl, isoxazolyl and pyrazinyl, wherein R 1Choose wantonly by methyl, sec.-propyl or ethyl replacement and/or (on carbon atom) and replaced by fluorine;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, pyridazinyl, thiazolyl and the pyrimidyl that replace;
R 3Be methyl, fluorine or chlorine;
R 2Be-CONR 4R 5
R 4And R 5Form together azetidinyl, 7-azabicyclo [2.2.1] heptan-7-base, morpholino or tetramethyleneimine basic ring.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group, pyridyl, isoxazolyl and pyrazinyl, wherein R 1Choose wantonly by methyl, sec.-propyl or ethyl replacement and/or (on carbon atom) and replaced by fluorine;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, pyridazinyl, thiazolyl and the pyrimidyl that replace;
R 3Be methyl, fluorine or chlorine;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl or tetramethyleneimine basic ring together.
In another aspect of this invention, provide the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group, pyridyl, isoxazolyl and pyrazinyl, wherein R 1Choose wantonly on the nitrogen-atoms by methyl, difluoromethyl, sec.-propyl or ethyl replaces and/or on carbon atom by fluorine or methyl substituted; Condition is if HET-1 is a pyrazolyl, so R 1Be selected from 1-methoxy propyl-2-base, sec.-propyl and tetrahydrofuran base;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, pyridazinyl, thiazolyl and the pyrimidyl that replace;
R 3Be methyl, fluorine or chlorine;
R 2Be-CONR 4R 5Or-SO 2NR 4R 5, especially-CONR 4R 5
R 4And R 5Form together azetidinyl, 7-azabicyclo [2.2.1] heptan-7-base, morpholino or tetramethyleneimine basic ring.
In another aspect of this invention, aspect A provides the formula (I) of preamble definition or (IA) compound, or its salt, wherein:
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from pyrazinyl (optional by methyl substituted), pyrazolyl (choosing wantonly on carbon by methyl substituted), methyl thiazolium di azoly (3-methyl isophthalic acid especially, 2,4-thiadiazoles-5-yl), thiazolyl (optional by methyl substituted), pyridyl (are optionally replaced) with isoxazolyl by fluorine; Condition is if HET-1 is a pyrazolyl, so R 1Especially be selected from 1-methoxy propyl-2-base, sec.-propyl and tetrahydrofuran base;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, pyridazinyl, thiazolyl and the pyrimidyl that replace;
R 3Be methyl, fluorine or chlorine;
R 2Be-CONR 4R 5Or-SO 2NR 4R 5, especially-CONR 4R 5
R 4And R 5Form together azetidinyl, 7-azabicyclo [2.2.1] heptan-7-base, morpholino or tetramethyleneimine basic ring.
In another aspect of this invention, aspect B provides the formula (I) of preamble definition or (IA) compound or its salt, wherein:
R 1Be selected from sec.-propyl, cyclopentyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, THP trtrahydropyranyl, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from pyrazinyl (optional by methyl substituted), pyrazolyl (choosing wantonly on carbon by methyl substituted), methyl thiazolium di azoly (3-methyl isophthalic acid especially, 2,4-thiadiazoles-5-yl), thiazolyl (optional by methyl substituted), pyridyl (are optionally replaced) with isoxazolyl by fluorine; Condition is if HET-1 is a pyrazolyl, so R 1Especially be selected from 1-methoxy propyl-2-base, sec.-propyl and tetrahydrofuran base;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl and the pyrazinyl that replace;
R 3Be methyl, fluorine or chlorine;
R 2Be-CONR 4R 5Or-SO 2NR 4R 5, especially-CONR 4R 5
R 4And R 5Form azetidinyl or tetramethyleneimine basic ring, especially azetidinyl together.
The specific compound of aspect B is formula (IB), (IC) and/or (ID) compound.
In another aspect of this invention, aspect C provides the formula (IB), (IC) of preamble definition or (ID) compound or its salt, wherein:
R 1Be selected from sec.-propyl, 1-hydroxyl third-2-base, tetrahydrofuran base and 1-methoxy propyl-2-base; (R especially 1Be selected from sec.-propyl, (2S)-1-hydroxyl third-2-base, tetrahydrofuran base and (2S)-1-methoxy propyl-2-yl);
HET-1 is the methylpyrazine base;
R 2Be-CONR 4R 5
R 4And R 5Form the azetidine basic ring together.
In another aspect of this invention, aspect D, formula (IB) compound or its salt that provides preamble to define, wherein:
R 1Be selected from sec.-propyl, 1-hydroxyl third-2-base, tetrahydrofuran base and 1-methoxy propyl-2-base; (R especially 1Be selected from sec.-propyl, (2S)-1-hydroxyl third-2-base, tetrahydrofuran base and (2S)-1-methoxy propyl-2-yl);
HET-1 is the methylpyrazine base;
R 2Be-CONR 4R 5
R 4And R 5Form the azetidine basic ring together.
In another aspect of this invention, aspect E provides the formula (IB), (IC) of preamble definition or (ID) compound or its salt, wherein:
R 1Be selected from sec.-propyl, tetrahydrofuran base and 1-methoxy propyl-2-base (especially (2S)-1-methoxy propyl-2-yl);
HET-1 is a pyrazolyl;
R 2Be-CONR 4R 5
R 4And R 5Form the azetidine basic ring together.
In another aspect of this invention, aspect F, formula (IB) compound or its salt that provides preamble to define, wherein:
R 1Be selected from sec.-propyl, tetrahydrofuran base and 1-methoxy propyl-2-base (especially (2S)-1-methoxy propyl-2-yl);
HET-1 is a pyrazolyl;
R 2Be-CONR 4R 5
R 4And R 5Form the azetidine basic ring together.
In another aspect of this invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from sec.-propyl, fourth-2-base, 1,3-difluoro third-2-base, 1-hydroxyl third-2-base and 1-methoxy propyl-2-base and tetrahydrofuran base;
HET-1 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
HET-2 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
R 2Be-SO 2NR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, 1-hydroxyl third-2-base and 1-methoxy propyl-2-base,
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl, wherein R 1Choose wantonly on carbon or nitrogen and replaced by methyl or ethyl;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, thiazolyl and the thienyl that replace;
R 3It is fluorine or chlorine;
R 2Be-SO 2NR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
HET-2 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
R 2Be-SO 2NR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl, and wherein R1 is optional is replaced by methyl, sec.-propyl or ethyl;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, thiazolyl and the pyrimidyl that replace;
R 3It is fluorine or chlorine;
R 2Be-SO 2NR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from sec.-propyl, 1,3-difluoro third-2-base, fourth-2-base, 1-hydroxyl third-2-base, 2-hydroxyl fourth-3-base, 1-difluoro-methoxy third-2-base, tetrahydrofuran base, 1-hydroxyl fourth-2-base and 1-methoxy propyl-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl, wherein R 1Optional by methyl, sec.-propyl or ethyl replacement;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, thiazolyl and the pyrimidyl that replace;
R 3It is fluorine or chlorine;
R 2Be-CONR 4R 5Or-SO 2NR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In one aspect of the invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
HET-2 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl, wherein R 1Choose wantonly on carbon or nitrogen and replaced by methyl or ethyl;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, thiazolyl and the thienyl that replace;
R 3It is fluorine or chlorine;
R 2Be-CONR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In one aspect of the invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
HET-2 is optional 5-that replaces or 6-unit heteroaryl ring, defines as preamble;
R 2Be-SO 2NR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
In another aspect of this invention, provide formula (I) (or IA) compound or its salt of preamble definition, wherein:
R 1Be selected from 1-fluorine methoxy propyl-2-base and 1,1-difluoro-methoxy third-2-base;
HET-1 is selected from thiazolyl, pyrazolyl, thiadiazolyl group and pyrazinyl, wherein R 1Choose wantonly on carbon or nitrogen and replaced by methyl or ethyl;
HET-2 is selected from by R 2Replacement and optional by R 3The pyridyl, pyrazinyl, thiazolyl and the thienyl that replace;
R 3It is fluorine or chlorine;
R 2Be-SO 2NR 4R 5
R 4And R 5Form azetidinyl, pyrrolidyl or morpholino ring together.
The further preferred compound of the present invention is each embodiment (and salt), and each described embodiment provides the present invention further aspect independently.Aspect further, the present invention also comprises any two or more compounds (and salt) of described embodiment.
Specific compound of the present invention comprises following any or multiple compound:
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
3-{[5-(azetidine-1-base alkylsulfonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[3-chloro-5-(morpholine-4-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide; With
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide; And/or
3-{[5-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[2-(azetidine-1-base carbonyl) pyrimidine-5-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[2-(azetidine-1-base carbonyl) pyrimidine-5-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1R, 2R)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-[1-(1-methylethyl)-1H-pyrazole-3-yl] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[4-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[4-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-(hydroxymethyl) propyl group] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide; And/or
3-{[2-(azetidine-1-base carbonyl) pyrimidine-5-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-(cyclopentyloxy)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-(cyclopentyloxy)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-(cyclopentyloxy)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1R, 2R)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[4-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-4-methyl isophthalic acid, the 3-thiazol-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-4-methyl isophthalic acid, the 3-thiazol-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-4-methyl isophthalic acid, the 3-thiazol-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-pyridine-2-yl-benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-[(1-methylethyl) the oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-[(1-methylethyl) the oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-[1-(1-methylethyl)-1H-pyrazole-3-yl]-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-[(1-methylethyl) the oxygen base] benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-pyridine-2-yl-benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the N-isoxazole-3-base-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-1H-pyrazole-3-yl benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1,5-dimethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1,5-dimethyl-1H-pyrazole-3-yl)-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridazine-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(4-methyl isophthalic acid, 3-thiazol-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(5-methyl isophthalic acid, 3-thiazol-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-[1-(1-methylethyl)-1H-pyrazole-3-yl]-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the N-1H-pyrazole-3-yl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1,5-dimethyl-1H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(4-methyl isophthalic acid, 3-thiazol-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(5-methyl isophthalic acid, 3-thiazol-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-[1-(1-methylethyl)-1H-pyrazole-3-yl]-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the N-1H-pyrazole-3-yl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(5-fluorine pyridine-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[3-chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide;
3-{[3-chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[3-chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide; With
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3R)-tetrahydrofuran (THF)-3-base oxygen base] benzamide; And/or
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the N-isoxazole-3-base-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-pyrazine-2-base-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(5-fluorine pyridine-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-pyridine-2-yl-benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the N-isoxazole-3-base-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(4-methyl isophthalic acid, 3-thiazol-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methyl isophthalic acid, 3-thiazol-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-pyrazine-2-yl-benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-pyrazine-2-yl-benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-pyrazine-2-yl-benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-pyrazine-2-yl-benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-pyrazine-2-yl-benzamide;
3-{[3-chloro-5-(morpholine-4-base carbonyl) pyridine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(7-azabicyclo [2.2.1] heptan-7-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide; With
3-{[5-(azetidine-1-base carbonyl)-4-methyl isophthalic acid, the 3-thiazol-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide; And/or
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-pyrazine-2-yl-benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-pyridine-2-yl-benzamide;
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide;
N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3-{[6-(tetramethyleneimine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-pyrazine-2-base-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(5-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the N-1H-pyrazole-3-yl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(5-methyl isophthalic acid H-pyrazole-3-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-1H-pyrazole-3-yl benzamide;
3-{[5-(azetidine-1-base alkylsulfonyl)-4-methyl isophthalic acid, the 3-thiazol-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-[1-(difluoromethyl)-1H-pyrazole-3-yl]-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide;
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-[1-(difluoromethyl)-1H-pyrazole-3-yl]-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-[1-(difluoromethyl)-1H-pyrazole-3-yl]-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide; And/or
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
Or its salt.
Another feature of the present invention is to comprise formula defined above (I), (IA), (IB), (IC) or (ID) pharmaceutical composition of compound or pharmaceutically acceptable salt thereof and pharmaceutically acceptable diluent or carrier.
According to another aspect of the present invention, provide formula defined above (I) as medicine, (IA), (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof.
According to another aspect of the present invention, provide as treatment by (I) defined above, (IA) of the disease of the GLK mediation especially medicine of type ii diabetes, (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof.
In addition according to the present invention, provide formula defined above (I), (IA), (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof be used for the treatment of by the application in the medicine of the disease of GLK mediation especially type ii diabetes in preparation.
The compounds of this invention can be formulated as aptly the pharmaceutical composition of such use.
According to another aspect of the present invention, by to needs like this formula (I), (IA), (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof of the Mammals effective dosage of treatment, provide treatment by the disease of the GLK mediation especially method of diabetes.
The disease specific of available The compounds of this invention or combination treatment comprises: do not follow blood sugar in the type ii diabetes of severe hypoglycemia danger to reduce (and effectively treatment I type), dyslipidemia, obesity, insulin resistance, metabolism syndrome X, glucose tolerance reduces (impairedglucose tolerance).
As described above, the GLK/GLKRP system can be described to (be of value to diabetes and fat) potential target spot of treatment " diabetes obesity ".Like this, according to the present invention on the other hand, provide formula (I), (IA), (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof, be used for combination therapy or prevention, especially treat the application of the medicine of diabetes and obesity in preparation.
According to another aspect of the present invention, formula (I), (IA), (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof are provided, preparation be used for the treatment of or prevent, application in the medicine of especially treatment of obesity.
According to another aspect of the present invention, provide as the formula defined above (I), (IA), (IB), (IC) of the medicine of treatment or prevention, especially treatment of obesity or (ID) compound or pharmaceutically acceptable salt thereof.
According to another aspect of the present invention,, provide the method for combination therapy obesity and diabetes by to needs like this formula (I), (IA), (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof of the Mammals effective dosage of treatment.
According to another aspect of the present invention,, provide the method for treatment of obesity by to needs like this formula (I), (IA), (IB), (IC) or (ID) compound or pharmaceutically acceptable salt thereof of the Mammals effective dosage of treatment.
Owing to have for example favourable physics and/or PK (pharmacokinetic) profile and/or favourable toxicity characteristic and/or favourable metabolic characteristic, The compounds of this invention so the especially suitable medicament of doing.
Favourable toxicity characteristic can for example prove by using Salmonella reversion test mensuration and/or anti-hERG ionic channel to test.Favourable metabolic characteristic means and for example reduces accretion rate, thereby cause reducing compound from intravital removing and be increased in exposure the compound thus, perhaps favourable metabolic characteristic can for example not form active metabolite (it is considered to is deleterious in some cases).
For example, the compound of aspect A-F can have favourable toxicology characteristic.
The present composition can adopt and be suitable for orally using (for example tablet, lozenge, hard or soft capsule, water-based or oil suspension, emulsion, dispersible powder or granule, syrup or elixir), the local form of using (for example, creme, ointment, gel or water or oil solution or suspensoid), the form of the form (for example, micro mist powder or liquid aerosol) of inhalation or parenterai administration (for example for the sterilization water-based oily solution of intravenously, subcutaneous or intramuscular administration or be used for the suppository of rectal administration).The formulation that is suitable for oral administration is preferred.
The present composition can pass through ordinary method, utilizes conventional medicine vehicle well known in the art to obtain.So the composition that is used to orally use can contain for example one or more tinting materials, sweeting agent, correctives and/or sanitas.
The suitable pharmaceutically acceptable vehicle that is used for tablet formulation for example comprises that inert diluent is lactose, yellow soda ash, calcium phosphate or lime carbonate for example; Granulation and disintegrating agent be W-Gum and alginic acid for example; Tackiness agent is starch for example; Lubricant is Magnesium Stearate, stearic acid or talcum powder for example; Sanitas is ethyl p-hydroxybenzoate or propyl ester for example, and oxidation inhibitor, for example xitix.Tablet formulation can be a dressing not, also can adopt dressing to change its disintegration and the activeconstituents follow-up sorption in gi tract, or improve its stability and/or outward appearance, and in any case, conventional Drug coating and the method that all can use this field to know.
The composition that orally uses can be the form of hard gelatin capsule, wherein for example lime carbonate, calcium phosphate or kaolin mix activeconstituents with inert solid diluent, or soft gelatin capsule form, wherein for example peanut oil, whiteruss or mixed with olive oil of activeconstituents and water or oil.
Aqueous suspension generally contains activeconstituents and one or more suspending agents of micro mist form, and described suspending agent for example is Xylo-Mucine, methylcellulose gum, Vltra tears, sodiun alginate, polyethylene-pyrrolidone, tragakanta and gum arabic; Disperse or wetting agent, the condenses of Yelkin TTS or alkylene oxide and lipid acid (for example polyoxyethylene stearic acid ester) for example, or the condensation product of oxyethane and long chain aliphatic alcohol, 17 oxidation ethylidene hexadecanols (heptadecaethyleneoxycetanol) for example, or oxyethane and derived from the condensation product of the partial ester of lipid acid and hexitol, polyoxyethylene Sorbitol Powder monooleate for example, or oxyethane and derived from the condensation product of the partial ester of lipid acid and hexitan, for example polyethylene sorbitan monooleate.Aqueous suspension also can contain one or more sanitass (for example ethyl p-hydroxybenzoate or propyl ester), oxidation inhibitor (for example xitix), tinting material, correctives and/or sweeting agent (for example sucrose, asccharin and aspartame).
Can prepare the oiliness suspensoid by activeconstituents being suspended in vegetables oil (for example peanut oil, sweet oil, sesame oil or Oleum Cocois) or the mineral oil (for example whiteruss).The oiliness suspensoid also can contain thickening material for example beeswax, solid paraffin or hexadecanol.Can add aforesaid sweeting agent and correctives so that good to eat oral preparations to be provided.These compositions can for example xitix be next anticorrosion by adding oxidation inhibitor.
Be fit to make the dispersible powder and the granule of aqueous suspension and generally contain activeconstituents and dispersion agent or wetting agent, suspending agent and one or more sanitass by adding entry.Suitable dispersion or wetting agent and suspending agent are as mentioned above.Also there are other vehicle for example sweeting agent, correctives and tinting material.
Pharmaceutical composition of the present invention also can adopt the form of oil-in-water emulsion.Oil phase can be a vegetables oil, for example sweet oil or peanut oil, perhaps mineral oil, for example whiteruss or their mixture.Suitable emulsifying agent for example can be, natural gum is gum arabic or tragacanth for example, natural phospholipid is soybean lecithin for example, with ester or partial ester (for example sorbitan monooleate) derived from lipid acid and hexitan, and the condensation product of described partial ester and oxyethane, for example polyoxyethylene sorbitan monooleate.Emulsion also can contain sweeting agent, correctives and sanitas.
Syrup and elixir can be prepared with sweeting agent (for example glycerine, propylene glycol, Sorbitol Powder, aspartame or sucrose), and also can contain negative catalyst, sanitas, correctives and/or tinting material.
Described pharmaceutical composition can also be the form of Injectable sterile water-based or oiliness suspensoid, and it can utilize one or more suitable dispersions or wetting agent and suspending agent to prepare according to currently known methods, and these reagent as mentioned above.Aseptic injection preparation also can be Injectable sterile water-based or the oiliness suspensoid in nontoxic parenteral acceptable diluent or solvent, for example solution in 1,3 butylene glycol.
Through the composition of inhalation, can be the conventional pressurised aerosol of the form that contains micro mist solid aerosol or drop that can the dispersed activity composition.Can use conventional aerosol propellent (for example volatility fluorinated hydrocarbons or hydrocarbon), the aerosol device is the quantitative device of dispersed activity composition normally.
Other information of relevant preparation can be with reference to the 5th volume of Comprehensive Medicinal Chemistry, 25.2 chapters (Corwin Hanschl; Chairman of Editorial Board), PergamonPress 1990.
Can be according to by treatment host and concrete route of administration different, determine with one or more mixed with excipients to prepare the amount of single dose form activeconstituents.For example, be used for promoting agent and suitable and vehicle convention amount (the accounting for the 5-98% of composition gross weight) that preparation to the human oral administration generally contains 0.5mg-2g for example.The activeconstituents that generally contains 1mg-500mg in the unit formulation approximately.About the further information of route of administration and dosage regimen can be with reference to the 5th volume of ComprehensiveMedicinal Chemistry, 25.3 chapters (Corwin Hanschl; Chairman ofEditorial Board), Pergamon Press 1990.
For the formula (I), (IA), (IB), (IC) of treatment or prevention purpose or (ID) dosage of compound, should be according to character and seriousness, animal or patient's age and the sex and the route of administration of illness, change according to the known principle of medicine.
Based on treatment or prevention purpose use formula (I) compound the time, generally be with per daily dose administration in the scope of for example 0.5mg-75mg/kg body weight, if needed can the divided dose administration.Adopt than low dosage during usually, with the parenteral route administration.So, for example when intravenous administration, generally adopt for example interior dosage of 0.5mg-30mg/kg weight range.Similarly, when inhalation, can adopt for example interior dosage of 0.5mg-25mg/kg weight range.Yet, the preferred oral administration.
The active rising of GLK of the present invention can be applicable in the treatment separately, perhaps with one or more other materials and/or indication treatment associating.Such combination therapy is when can treat component by each, the mode of order or separate administration reaches.Treatment simultaneously can be adopted single tablet or adopt tablet form separately.For example, in treatment of diabetes, chemotherapy can comprise the treatment of following main kind:
1) Regular Insulin and insulin analog;
2) Regular Insulin succagoga comprises sulfonylurea (for example Glyburide, Glipizide), meals glucose conditioning agent (for example repaglinide, nateglinide);
3) medicine (for example inhibitors of dipeptidyl IV, GLP-I agonist) of promotion incretin effect;
4) insulin sensitizer comprises PPAR gamma agonist (for example pioglitazone and rosiglitazone) and has the material of PPAR α and PPAR gamma activity;
5) regulate hepatic glucose equilibrated medicine (for example N1,N1-Dimethylbiguanide, 1,6-hexose diphosphate inhibitor, glycogen phosphorylase inhibitors, glycogen synthase kinase enzyme inhibitors);
6) medicine (for example acarbose) of glucose absorption in the minimizing intestines;
7) prevent that glucose is by the resorbent medicine of kidney (SGLT inhibitor);
8) medicine (for example aldose reductase inhibitor) of the long-term hyperglycemia complication of treatment;
9) anti-obesity medicine (for example sibutramine and orlistat);
10) lipotropism dyslipidemia medicine, for example HMG-CoA reductase inhibitor (for example Statins); PPAR alfa agonists (chlorine Bei Te, for example gemfibrozil); Cholic acid chelating agent (Colestyramine); Cholesterol absorption inhibitor (plant Sitosterol (stanol), synthetic inhibitor); Cholic acid absorption inhibitor (IBATi) and nicotinic acid and analogue (nicotinic acid and sustained release preparation);
11) antihypertensive drug, for example beta-blocker (for example atenolol USP 23, Proprasylyte); ACE inhibitor (for example lisinopril); Calcium antagonist (for example Nifedipine); Angiotensin receptor antagonist (for example Candesartan), alpha-2 antagonists and diuretic(s) (for example Furosemide and benzthiazide);
12) extravasated blood (Haemostasis) conditioning agent, antithrombotic drug for example, Fibrinolytic activator and antiplatelet drug; The zymoplasm antagonist; The Xa factor inhibitor; The VIIa factor suppresses); Antiplatelet drug (for example Asprin, clopidogrel); Antithrombotics (heparin and lower molecular weight analogue, r-hirudin) and warfarin;
13) medicament of the effect of antagonism hyperglycemic-glycogenolytic factor; With
14) anti-inflammatory agent, for example nonsteroidal anti-inflammatory (for example Asprin) and steroid antiphlogiston (for example cortisone).
According to another aspect of the present invention, provide each compound and its salt of the final product for preparing in the following example.
Can be by being used to prepare any currently known methods of related compound on this compounds or the structure, preparation The compounds of this invention or its salt.Can utilize ordinary method, functional group is protected and deprotection.The for example amino and carboxylic acid protecting group's of protecting group example (and the mode of formation and last deprotection), referring to T.W.Greene and P.G.M.Wuts, " Protective Groups in OrganicSynthesis ", the 2nd edition, John Wiley ﹠amp; Sons, New York, 1991.
Provide synthesis type (I), (IA), (IB), (IC) or (ID) method of compound as another aspect of the present invention.Therefore, according to another aspect of the present invention, preparation formula (I), (IA), (IB), (IC) or (ID) method of compound are provided, this method comprise method a)-e) (except as otherwise noted, otherwise wherein each variable as previous its in formula (I), (IA), (IB), (IC) or (ID) definition in the compound):
(a) make formula (III) acid or its activated derivatives and the reaction of formula (IV) compound, wherein R 1As the form that preamble defines or it has been protected;
Figure A200810169286D00491
Or
(b) make the reaction of formula V compound and formula (VI) compound,
R 1—X 1
Figure A200810169286D00492
(V) (VI)
X wherein 1Be leavings group and X 2Be hydroxyl, perhaps X 1Be hydroxyl and X 2Leavings group, and R wherein 1As the form that preamble defines or it has been protected;
Method (b) also can by other places that describe with method well known to those skilled in the art, use intermediate ester formula (VII), wherein P 1Be the protecting group of hereinafter describing, ester hydrolysis that continues and acid amides form to be realized.
R 1—X 1
Figure A200810169286D00493
(V) (VII)
Or
(c) make the reaction of formula (VIII) compound and formula (IX)
Figure A200810169286D00501
X wherein 3Be leavings group or organometallic reagent and X 4Be hydroxyl, perhaps X 3Be hydroxyl and X 4Be leavings group or organometallic reagent, and R wherein 1As the form that preamble defines or it has been protected;
Method (c) also can by other places that describe with method well known to those skilled in the art, use intermediate ester formula (X), ester hydrolysis and acid amides form and realize then;
Figure A200810169286D00502
(d) make the reaction of formula (XI) compound and formula (XII) compound,
Figure A200810169286D00503
X wherein 5It is leavings group; And R wherein 1As the form that preamble defines or it has been protected; Perhaps
E) make formula (XIII) compound
R wherein 2aBe R 2Precursor, for example carboxylic acid, ester or acid anhydrides are (for R 2=-CONR 4R 5) or the sulfonic acid equivalent (for R 2Be-SO 2NR 4R 5); With formula-NR 4R 5The amine reaction;
And thereafter, if necessary:
I) formula (I) compound is converted into another formula (I) compound;
Ii) remove any protecting group; And/or
Iii) form its salt.
For method b)-d), suitable leavings group X 1-X 5Be any leavings group that is used for these type reaction known in the art, for example halogen, alkoxyl group, trifyl oxygen base, methylsulfonyl oxygen base or p-toluenesulfonyl oxygen base; Perhaps original position can be converted into the group (for example hydroxyl) of leavings group (for example oxygen base triphenyl phosphonium (oxygen base triphenyl phosphonium)).
The R that contains the hydroxyl of having protected 1The appropriate value hydroxyl that is any suitable protection known in the art, for example simple ether, for example methyl ether, tertbutyl ether or silyl ether alkyl for example-OSi[(1-4C)] 3(wherein each (1-4C) alkyl is independently selected from methyl, ethyl, propyl group, sec.-propyl and the tertiary butyl).The example of this class trialkylsilkl is trimethyl silyl, triethylsilyl, triisopropyl silyl and t-butyldimethylsilyl.Other suitable silyl ether is those silyl ethers that contain the phenyl of phenyl and replacement, for example-Si (PhMe2) and-Si (TolMe 2) (wherein Tol=toluene).Other suitable hydroxyl protecting group provides hereinafter.
Formula (III)-(XII) compound can have been bought from the market, or known in the art, perhaps can pass through methods known in the art, for example shown in the subsidiary embodiment or the method that describes below prepare.About the preparation method's of described compound more information, see also our PCT application WO 03/000267, WO 03/015774 and WO 03/000262 and reference wherein.Usually, be understandable that, randomly in the presence of suitable alkali,, can form any aryl-O or alkyl-O key by the method for nucleophilic substitution or metal catalytic.
Can be by method for example a) to d) shown in method, and/or by the above-mentioned method that is used for preparation formula (III)-(XII) compound, preparation formula (XIII) compound.
Can be according to R 1The character of group is by making the reaction of suitable precursor and formula V compound or derivatives thereof, for example by the leavings group X in the nucleophilic displacement formula V compound 1, come drawing-in system (III), (IX), (X), (XI) and (XIII) radicals R in the compound 1The formula V compound is normally commercially available, perhaps can be made by commercially available compound by the change of simple functional group, perhaps makes by literature method.More information can obtain in WO2004/076420, WO2005/054200, WO2005/054233, WO 2005/044801 and WO 2005/056530.Reaction scheme below and/or in additional embodiment, provided some and use different R 1The illustrative example of group, and this illustrative example is in the ordinary course of things, does not have the R of explanation below can being applicable to similarly by methods known in the art 1Group, described method be referring to for example Bull.Chem.Soc.Japan, and 73 (2000), 471-484, International Patent Application WO2002/050003 and Bioorganic and Medicinal Chemistry Letters, (2001), 11,407.
Figure A200810169286D00521
Reaction scheme 1
Figure A200810169286D00522
Reaction scheme 2
Figure A200810169286D00523
Reaction scheme 3
Figure A200810169286D00531
Reaction scheme 4
[PG is a protecting group, and Ts is a p-toluenesulfonyl].
Formula (I) compound is converted into the example of the method for another kind of formula (I) compound, be well known in the art, for example comprise functional group's change, hydrolysis, hydrogenation, hydrogenolysis, oxidation or reduction, and/or by standard reaction for example the coupling of acid amides or metal catalytic do further functionalized, perhaps nucleophilic displacement reaction.The other example that formula (I) compound is converted into another kind of formula (I) compound is to use the reaction as illustrating in the reaction scheme 4, with R 1In HM forming methoxyl group, and for example with R 1In hydroxymethyl (for example at R 1Be under the situation of hydroxyl third-2-base) be converted into difluoro-methoxy.
Be appreciated that substituent R 3, R 6And/or R 7Can be in building-up process any stage easily be incorporated in the molecule, perhaps may reside in the parent material.A)-e) in the step, the precursor of one of these substituting groups can be present in intramolecularly, is converted into the substituting group of expectation then, as the last step that forms formula (I) compound at aforesaid method; Then, in the case of necessary
I) formula (I) compound is converted into another kind of formula (I) compound;
Ii) remove any protecting group; And/or
Iii) form its salt.
The concrete reaction conditions of above-mentioned reaction is as follows, if P wherein 1Be protecting group, P so 1Preferably (1-4C) alkyl, for example methyl or ethyl:
Method a)-form the amino of acid amides and the linked reaction of carboxylic acid is well-known in the art.For example,
(i) use suitable linked reaction, for example, under the room temperature, for example in methylene dichloride (DCM), chloroform or the dimethyl formamide (DMF), carbodiimide carbon and EDAC (1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride) carry out coupled reaction in suitable solvent; Or
(ii) suitable solvent for example DCM in the presence of, with oxalyl chloride reaction, carboxyl is activated into chloride of acid.Then, between 0 ℃-80 ℃, alkali for example triethylamine or pyridine in the presence of, for example among chloroform or the DCM, make the reaction of chloride of acid and formula (IV) compound in suitable solvent.
Method b)-can make formula V and (VI) compound in The suitable solvent for example in DMF or the tetrahydrofuran (THF) (THF), between temperature 0-200 ℃, for example sodium hydride or potassium tert.-butoxide one react optional microwave heating or metal catalyst for example acid chloride (II), palladium on carbon, venus crystals (II) or the cupric iodide (I) of using with alkali; Perhaps, can make formula V and (VI) at suitable solution for example among THF or the DCM, with suitable phosphine for example triphenyl phosphine and azo and carboxylicesters for example azo diacid diethyl phthalate one react; Method b) also can adopt the precursor of formula (VII) ester, for example aryl-nitrile or trifluoromethyl derivative are converted into carboxylic acid then as mentioned before and form acid amides and carry out;
Method c)-can make formula (VIII) and (IX) compound in The suitable solvent for example among DMF or the THF, between temperature 0-200 ℃, for example sodium hydride or potassium tert.-butoxide one react optional microwave heating or metal catalyst for example acid chloride (II), palladium on carbon, venus crystals (II) or the cupric iodide (I) of using with alkali; Method c) for example aryl-nitrile or trifluoromethyl derivative of the precursor that also can use formula (X) ester, being converted into carboxylic acid as mentioned before and forming acid amides of continuing carried out;
Formula (VIII) compound can have been bought from the market, perhaps can be by method well-known in the art, for example functional group's change (for example hydrolysis, hydrogenation, hydrogenolysis, oxidation or reduction), and/or by standard reaction (for example coupling of acid amides or sulphonamide or metal catalytic, perhaps nucleophilic displacement or close electric replacement(metathesis)reaction) further functionalized and/or cyclisation, prepare by commercially available material;
Method d)-and the reaction of Shi (XI) compound and formula (XII) can carry out under the following conditions: containing highly basic for example polar solvent for example DMF or the non-polar solvent for example among the THF of sodium hydride or potassium tert.-butoxide, between temperature 0-200 ℃, optional microwave heating or metal catalyst for example acid chloride (II), palladium on carbon, venus crystals (II) or the cupric iodide (I) of using;
Method e)-form the amino of acid amides and the linked reaction of carboxylic acid or sulfonic acid or acid derivative is well-known, and by above a) being described for method.
Formula (III), (VI), (VII), (IX) and/or some intermediate (XI) it is believed that it is new, and have constituted independent aspects of the present invention.
R wherein 1It is believed that it is new as the defined formula of this paper formula (I) compound (III), (IX) and/or some intermediate (XI), and constitute independently aspect of the present invention.
Some intermediate of formula (XIII) it is believed that to be new and to constitute the present invention aspect independently.
In preparation process, it is favourable using the protecting group of functional group in the molecule.Protecting group can by describe in the document or the known any method easily that is suitable for removing above-mentioned protecting group of this area chemist remove; can be selected such method, thereby be realized sloughing protecting group and the minimum interference of other groups in the molecule.
For convenience's sake, provided the specific examples of protecting group below, wherein " rudimentary " is meant that used group preferably has 1-4 carbon atom.Should be understood that described example is non exhaustive.The specific examples that is used to remove the method for protecting group equally also is non exhaustive.Protecting group of not mentioning especially and deprotection method are also within the scope of the present invention.
Carboxyl-protecting group can be to form the aliphatic series of ester or the residue of aromatic grease group alcohol, perhaps can form the residue (described alcohol or silanol preferably comprise 1-20 carbon atom) of the silanol of ester.The example of carboxyl-protecting group comprises straight or branched (1-12C) alkyl (for example sec.-propyl, the tertiary butyl); Lower alkoxy low-grade alkyl group (for example methoxymethyl, ethoxymethyl, isobutoxy methyl); Lower aliphatic acyloxy low alkyl group (for example acetoxy-methyl, propionyloxy methyl, butyryl acyloxy methyl, pivalyl yloxymethyl); Elementary alkoxy carbonyl oxygen base low alkyl group (for example 1-methoxycarbonyl oxygen base ethyl, 1-ethoxy carbonyl oxygen base ethyl); Aromatic yl elementary alkyl (for example to methoxy-benzyl, adjacent nitrobenzyl, to nitrobenzyl, diphenyl-methyl and phthalidyl); Three (low alkyl group) silyl (for example three silyls and tertiary butyl dimethyl silanyl); Three (low alkyl group) silyl low alkyl group (for example trimethyl silyl ethyl); (2-6C) thiazolinyl (for example allyl group and vinyl ethyl).
The method that is particularly suitable for removing carboxyl-protecting group comprise for example acid-, metal-or enzymatic-catalytic hydrolysis.Also can use hydrogenization.
The example of hydroxyl protecting group comprises: methyl, low-grade alkenyl (for example allyl group); Low-grade alkane acidyl (for example ethanoyl); Elementary alkoxy carbonyl (for example tert-butoxycarbonyl); Low-grade alkenyl oxygen base carbonyl (for example allyloxy carbonyl); Aryl-lower alkoxy carbonyl (for example benzoyl oxygen carbonyl, to methoxyl group benzyloxy carbonyl, ortho-nitrophenyl methoxycarbonyl, p-nitrophenyl methoxycarbonyl); Three lower alkyl/aryl groups silyls (for example trimethyl silyl, t-butyldimethylsilyl, t-butyldiphenylsilyl); Aromatic yl elementary alkyl (for example benzyl); With triaryl low alkyl group (for example trityl group).Tetrahydropyrans-2-base; Aromatic yl elementary alkyl (for example benzyl); With triaryl low alkyl group (for example trityl group).The example of amino protecting group comprises formyl radical, aralkyl (for example the benzyl of benzyl and replacement for example, to methoxy-benzyl, nitrobenzyl and 2,4-dimethoxy-benzyl and trityl group); Two-to anisyl methyl and furyl methyl; Elementary alkoxy carbonyl (for example tert-butoxycarbonyl); Low-grade alkenyl oxygen base carbonyl (for example allyloxy carbonyl); The aryl-lower alkoxy carbonyl (for example benzyl oxygen base carbonyl, to methoxy-benzyl oxygen base carbonyl, adjacent nitrobenzyl oxygen base carbonyl, to nitrobenzyl oxygen base carbonyl; Trialkylsilkl (for example trimethyl silyl and t-butyldimethylsilyl); Alkylidene group (for example methylene radical); The benzylidene of benzylidene and replacement.
The method that is suitable for removing hydroxyl and amino protecting group comprises; for example; hydrogenation, nucleophilic substitution, acid-, alkali-, metal-or enzymatic-catalytic hydrolysis, perhaps for can perhaps using fluoride ion with catalytic hydrogenolysis or photodissociation for silyl such as adjacent nitrobenzyl oxygen base carbonyl.For example, can take off the methyl ether protecting group of hydroxyl by the iodate trimethyl silyl.Can take off the tertbutyl ether protecting group of hydroxyl by hydrolysis, for example use the hydrogenchloride in methyl alcohol.
The example of the protecting group of amide group comprises aralkoxy methyl (for example benzyl oxygen ylmethyl of benzyl oxygen ylmethyl and replacement); Alkoxy methyl (for example methoxymethyl and trimethylsilylethoxymethyl); Trialkyl/aryl silyl (for example trimethyl silyl, t-butyldimethylsilyl, t-butyldiphenylsilyl); Trialkyl/aryl silyl oxygen ylmethyl (for example t-butyldimethylsilyl oxygen ylmethyl, t-butyldiphenylsilyl oxygen ylmethyl); 4-alkoxyl phenyl (for example 4-p-methoxy-phenyl); 2,4-two (alkoxyl group) phenyl (for example 2,4-Dimethoxyphenyl); 4-alkoxybenzyl (for example 4-methoxy-benzyl); 2,4-two (alkoxyl group) benzyl (for example 2,4-two (methoxyl group) benzyl); With 1-thiazolinyl (for example the vinyl of allyl group, but-1-ene base and replacement for example 2-phenyl vinyl).
By amide group and suitable aralkoxy methyl chloride reaction, the aralkoxy methyl can be introduced on the amide group, and can be sloughed by catalytic hydrogenation.React by the muriate that makes acid amides and suit, and remove, can introduce alkoxy methyl, trialkyl/aryl silyl and trialkyl/silyl oxygen ylmethyl with acid; Perhaps under the situation that contains silyl-group, fluoride ion.By carrying out arylation or alkylated reaction, can introduce alkoxyl phenyl and alkoxybenzyl easily, and can take off by carrying out oxidizing reaction with ceric ammonium nitrate with the halogenide that suits.At last, by acid amides and suitable aldehyde reaction, can introduce alkane-1-thiazolinyl, and usable acid is removed.
In above-mentioned other medicines composition, step, method, application and medication preparation feature, the other and preferred aspect and the embodiment of The compounds of this invention described herein also can be suitable for.
The following examples are for the illustrations purpose, but not intention restriction the application's scope.The compounds represented of each example a specific and aspect independently of the present invention.In following indefiniteness embodiment, unless otherwise stated, otherwise:
(i) evaporation is undertaken by rotary evaporation in a vacuum, and aftertreatment is to be undertaken by after removing by filter the residual solid such as siccative;
(ii) operation is promptly for example being carried out under argon gas or the nitrogen between 18-25 ℃ and at rare gas element in room temperature;
(iii) the yield of giving only for the usefulness of example explanation, maximum yield not necessarily;
(iv) except as otherwise noted, the structure of formula (I) final product, be through nuclear (being generally proton) mr (NMR), adopt certain field intensity (for proton), for example 300MHz (adopting VarianGemini 2000 usually) or 400MHz (adopting Bruker Avance DPX400 usually), and confirm through mass-spectrometric technique; The chemical displacement value of proton NMR spectrum is that unit measures with δ, adopts the multiplicity at following abbreviation expression peak: s, and is unimodal; D, doublet; T, triplet; M, multiplet; Br, broad peak; Q, quartet; Quin, quintet;
(v) generally without identifying fully, its purity adopts thin-layer chromatography (TLC), high performance liquid chromatography (HPLC), infrared (IR) or NMR to analyze and measures intermediate;
(vi) except as otherwise noted, chromatography purification typically refers to the silicon-dioxide flash column chromatography.Column chromatography adopts the pre-silica column of loading (4g is to reaching as high as 400g), for example Redisep usually TM(for example available from Presearch Ltd, Hitchin, Herts, UK) or Biotage (Herts UK), adopts Biotage pump and fraction gathering system wash-out for BiotageUK Ltd, Hertford.Solid phase extraction (SPE) purifying typically refers to the chromatographic column that adopts the pre-SPE of filling material, for example
Figure A200810169286D0057182947QIETU
The SCX-2 post (for example available from International Sorbent Technology Ltd, Dryffryn Busi ness Park, Hengoed, Mid Glamorgan, UK);
(vii) mass spectrum (MS) data are to generate in the LCMS system, and wherein the HPLC assembly generally includes Agilent 1100 or Waters Alliance HT (2790 ﹠amp; 2795) equipment, and at Phemonenex Gemini C18 5 μ m, 50 x 2mm posts (or similar post) are gone up operation, (for example adopt acid elutriant wash-out, adopt 0-95% water/acetonitrile to carry out gradient elution, it is 1% formic acid at 50:50 water that 5% eluent is wherein arranged: the solution in acetonitrile (v/v) mixture; Perhaps adopt and contain the suitable solvent systems that methyl alcohol replaces acetonitrile), perhaps alkaline eluant wash-out (for example, adopt 0-95% water/acetonitrile to carry out gradient elution, it is the solution of 0.1%880 Ammonia in the acetonitrile mixture that 5% eluent is wherein arranged); Generally include Waters ZQ, spectrometer with the MS assembly.Electron spray(ES) (Electrospray) (ESI) positive and negative base peak intensity chromatogram and UV always absorbs chromatogram and generates between 220-300nm, and provides with m/z; Usually, only the ion of parent quality (parentmass) is indicated in report, and the value that is provided is (M-H) except as otherwise noted -
(viii) Shi Yi microwave reactor comprises " Smith Creator ", " CEM Explorer ", " Biotage Initiator sixty " and " Biotage Initiator eight ";
(ix) fusing point test is carried out with dsc (DSC); Analysis is to use the equipment such as Mettler DSC822e or Mettler DSC820 to carry out.Will be less than usually the sample (being contained in 40 μ L is equipped with in the aluminium dish with the perforation lid) of 5mg material, with the fixedly heating rate of 10 ℃ of per minutes in heating between 25 ℃-325 ℃ of the temperature ranges.The sweeping gas that adopts nitrogen is to use with 100mL/ minute flow rate.Be appreciated that at an instrument and arrive between another sample to an other method or a sample that the beginning of DSC and/or maximum temperature value can slightly change, so should not be interpreted as it is absolute to the value of quoting to a kind of instrument, a method.Be appreciated that some sample can be a solvate, and it also can influence fusing point.
Shortenings
The DCM methylene dichloride
The DEAD diethylazodicarboxylate
The DIAD diisopropyl azo-2-carboxylic acid
DIPEA N, the N-diisopropyl ethyl amine
The DMA N,N-DIMETHYLACETAMIDE
The DMSO dimethyl sulfoxide (DMSO)
The DMF dimethyl formamide
EDAC 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride
HATU O-(7-azepine benzo triazol-1-yl)-N, N, N ', N '-tetramethyl-urea hexafluorophosphate
The HPLC high pressure liquid chromatography
The HPMC HYDROXY PROPYL METHYLCELLULOSE
The LCMS liquid chromatography/mass spectrometry
NMP N-N-methyl-2-2-pyrrolidone N-
The NMR nuclear magnetic resonance spectrum
The RT room temperature
The THF tetrahydrofuran (THF)
The TFA trifluoroacetic acid
CDCl 3Contain deuteriochloroform (deuterochloroform)
MgSO 4Sal epsom
NaHMDS sodium hexamethyldisilazide (sodium hexamethyldisilazide)
The name of ACD NAME computer package is all adopted in the name of all compounds.
Embodiment 1:3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D00591
With 3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-1, (0.15g, 0.33mmol) mixture in methyl alcohol (10mL) and 1M hydrochloric acid (10mL) was in stirring at room 90 minutes for 3-thiazol-2-yl benzamide.Remove volatile matter in the vacuum, make resistates reach pH6, be extracted into then in the ethyl acetate (3 x 30mL) with saturated sodium bicarbonate aqueous solution, and with the organic layer water (30mL), salt solution (30mL) washing that merge, dry (MgSO 4), filter and remove in a vacuum and desolvate, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (65mg) with the mixture gradient elution of 0-10% methyl alcohol in ethyl acetate.
1H?NMR?δ(CDCl 3)：1.32(d，3H)，2.30(s，1H)，2.39(quin，2H)，3.79(m，2H)，4.20-4.40(brm，4H)，4.58(m，1H)，6.98(m，2H)，7.27(m，1H)，7.35(m，1H)，7.39(s，1H)，7.41(s，1H)，8.11(d，1H)，8.41(s，1H)，11.50(s，1H).m/z?455(M+H) +
Following compound is to adopt similar method by suitable silyl ether synthetic.
Figure A200810169286D00592
Figure A200810169286D00601
The 3-{[5-that uses among the embodiment 1 (azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-1, the preparation method of 3-thiazol-2-yl benzamide is described below:
3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D00602
Salt of wormwood (0.14g, 0.98mmol) be added to 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-1,3-thiazol-2-yl benzamide (200mg, 0.49mmol) and 5-(azetidine-1-base carbonyl)-2-chloropyridine (96mg, 0.49mmol) in the mixture in acetonitrile (5.0mL), and the mixture that will stir is ' among the Biotageinitiator Microwave ' in 160 ℃ of heating 4 hours.Make mixture reach room temperature, and between ethyl acetate (50mL) and water (50mL), distribute.Ethyl acetate layer is separated water (5 x 50mL), salt solution (50mL) washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide, the mixture gradient elution with in the 60-100% ethyl acetate isohexane has obtained required compound (0.15g).
m/z?569(M+H) +
The precursor of embodiment 1a is to adopt similar method by 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-1,3-thiazol-2-yl benzamide is used 5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine:
Figure A200810169286D00611
The precursor of preparation embodiment 1b as described below:
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D00612
Cesium carbonate (0.478g, 1.47mmol) be added to 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-1,3-thiazol-2-yl benzamide (200mg, 0.49mmol), bromine three (triphenylphosphine) copper (46mg, 0.049mmol) and 2-(azetidine-1-base carbonyl)-5-bromopyridine (130mg, 5.39mmol) in the mixture in DMA (5.0mL), and the mixture that will stir is ' among the Biotage initiator Microwave ' in 160 ℃ of heating 3 hours.Make mixture reach room temperature and normal pressure, and between ethyl acetate (50mL) and water (50mL), distribute.Ethyl acetate layer is separated water (5 x 50mL), salt solution (50mL) washing, dry (MgSO 4) and flash to resistates, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-100% ethyl acetate in isohexane, has been obtained required compound (47mg).
1H?NMR?δ(CDCl 3)：0.00(s，3H)，0.03(s，3H)，0.83(s，9H)，1.29(d，3H)，2.32(quin，2H)，3.65-3.80(m，2H)，4.21(t，2H)，4.46(m，1H)，4.68(t，2H)，6.81(s，1H)，6.92(m，1H)，7.25(m，1H)，7.30-7.50(m，2H)，7.65(m，1H)，8.08(d，1H)，8.28(s，1H)，11.50(brs，1H).
m/z?569(M+H) +
The precursor of embodiment 1c-1d is to adopt similar method by 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[((1S)-1-methyl-2-{[three (1-methylethyl) silyl] the oxygen base } ethyl) the oxygen base] benzamide uses suitable bromopyridine preparation:
Figure A200810169286D00621
Can be according to Synthetic Communications, 31 (18), 2865-2879 (2001) prepares bromine three (triphenylphosphine) copper.The preparation of bromine three (triphenylphosphine) copper is described below.
Bromine three (triphenylphosphine) copper
(111.7g 0.5mol) is added to the triphenylphosphine that stirring (557.4g, 2.13mol) in the solution in methyl alcohol (1.85L) (observe heat release to 32 ℃) in batches cupric bromide (II) in room temperature.To react and stir 10 minutes, be heated to 65 ℃ then, and cool overnight.With the solid filtering collection, with ethanol (5vol), ether (3 x 5vol) washing, under vacuum, use dry 2 hours of sinter, in vacuum chamber, spend the night acquisition crystalline solid product (460g) then in 30 ℃.
At the precursor 3-{[5-of preparation embodiment 1 (azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-1, the preparation of the 5-that uses in the 3-thiazol-2-yl benzamide (azetidine-1-base carbonyl)-2-chloropyridine is described below:
5-(azetidine-1-base carbonyl)-2-chloropyridine
Figure A200810169286D00622
DMF (2) be added to the 6-chlorine apellagrin (1.00g, 6.35mmol) and oxalyl chloride (0.67mL, 7.62mmol) (3.15mL is 6.35mmol) in the solution at ether at DCM (20mL) and 2M hydrogenchloride.In stirring at room 4 hours, and DCM and excessive oxalyl chloride were removed in evaporation in a vacuum with mixture.Residual chloride of acid is dissolved among the DCM (10mL), and be added to the azetidine hydrochloride (0.66g, 6.99mmol) and triethylamine (2.14mL is 13.97mmol) in the mixture in DCM (10mL), then in stirring at room 24 hours.Remove DCM in the vacuum, and resistates is distributed between ethyl acetate (100mL) and 1M citric acid (50mL).Ethyl acetate layer is used saturated sodium bicarbonate aqueous solution (50mL) and salt solution (50mL) washing successively, dry (MgSO4), and remove in a vacuum and desolvate, obtain resistates, it is composed purifying in the enterprising circumstances in which people get things ready for a trip of silicon-dioxide, with the mixture gradient elution in the 50-100% ethyl acetate isohexane, obtained required compound (0.73g). 1H?NMR?δ(CDCl 3)2.43(quin，2H)，4.2-4.4(brm，4H)，7.42(d，1H)，7.97(d，1H)，8.63(s，1H).m/z?197(M+H) +
The haloperidid that uses in the precursor of preparation embodiment 1a-1d is to adopt similar method to be prepared by suitable nicotinic acid:
Figure A200810169286D00631
Described nicotinic acid is compound known in the compound that can buy from the market or the document.2-(azetidine-1-base carbonyl)-5-bromopyridine reference (Heterocycles, 15 (1) 1981,213-23).
The 3-[((1S of use in embodiment 1,1a and 1b synthetic)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-preparation of 5-hydroxy-n-1,3-thiazoles-2-yl-benzamide is described below:
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-1,3-thiazoles-2-yl-benzamide
Figure A200810169286D00641
The lithium hydroxide monohydrate aqueous solution (0.304g, 7.25mmol) be added to 3-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide (1.552g, 2.90mmol) in the solution in THF (40mL), and in stirred overnight at room temperature.Remove THF in the vacuum, and resistates is adjusted to pH7 by adding 1N hydrochloric acid (7.25mL).Gum forms, and solution is filtered, and gum is dissolved in the methyl alcohol.Filtrate is distributed with ethyl acetate (75mL), and with organism salt water washing, dry (MgSO 4), merge with methanol solution, and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required product (0.865g) with the mixture wash-out of 40% ethyl acetate in isohexane.
1H NMR δ (CDCl 3) :-0.02 (d, 6H), 0.81 (s, 9H), 1.24 (d, 3H), 3.67 (m, 2H), 4.43 (sextet, 1H), 6.65 (t, 1H), 7.00 (d, 1H), 7.13 (t, 1H), 7.15 (t, 1H), 7.40 (d, 1H) .m/z 409 (M+H) +
3-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D00642
Tert-butyldimethylsilyl chloride (1.66g, 11.0mmol) and imidazoles (1.78g, 26.0mmol) be added to 3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl] the oxygen base-N-1,3-thiazol-2-yl benzamide (1.541g, 5.24mmol) in the solution in DMF (10mL), and will be reflected under the silicon-dioxide drying tube in stirred overnight at room temperature.Add entry (30mL), and solution is distributed with ethyl acetate (100mL).Organic phase salt water washing, dry (MgSO 4) and be evaporated to resistates, with its chromatogram purification on silicon-dioxide,, obtained required product (1.525g) with the mixture wash-out of 15% ethyl acetate in isohexane. 1H NMR δ (CDCl 3) :-0.17 (d, 6H), 0.00 (s, 6H), 0.65 (s, 9H), 0.76 (s, 9H), 1.08 (d, 3H), 3.50 (m, 2H), 4.25 (sextet, 1H), 6.43 (t, 1H), 6.77 (d, 1H), 6.83 (t, 1H), 6.96 (t, 1H), 7.06 (d, 1H) .m/z 423 (M-H) -
The 3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D00651
At the following iodine of rare gas element (trimethylammonium) silane (7.19mL, 50.5mmol) be added drop-wise to 3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base-N-1,3-thiazol-2-yl benzamide (3.116g, 10.1mmol) in the solution in acetonitrile (60mL), and will react on stirred overnight at room temperature.Add methyl alcohol (5mL), and will react restir 10 minutes.Add saturated sodium thiosulfate solution (5mL) and unsaturated carbonate potassium solution (5mL), and will react restir 10 minutes.Remove organic solvent in the vacuum, and distribute solution to extract gained with ethyl acetate (100mL).By adding 1M hydrochloric acid water layer is adjusted to pH5, and uses ethyl acetate extraction.The organic layer that merges is washed dry (MgSO with salt solution (20mL) 4), and evaporation, with resistates chromatogram purification on silicon-dioxide, eluent ethyl acetate has obtained required compound (1.541g).
1H NMR δ (d 6-DMSO): 1.23 (d, 3H), 3.52 (m, 2H), 4.50 (sextet, 1H), 6.55 (t, 1H), 7.04 (t, 1H), 7.18 (t, 1H), 7.27 (d, 1H), 7.55 (d, 1H), 12.47 (s, 1H) .m/z 295 (M+H) +
The 3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-N-1,3-thiazol-2-yl benzene first
Acid amides
Figure A200810169286D00652
With 3-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-the 5-{[(2-aminomethyl phenyl) methyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide (6.9g) and the solution of thioanisole (10mL) in trifluoroacetic acid (65mL) were in stirring at room 16 hours.Remove trifluoroacetic acid in the vacuum, and remaining oily matter is distributed between ethyl acetate (75mL) and sodium bicarbonate aqueous solution (200mL).Water layer is separated, with ethyl acetate (2 x 75mL) extraction, and with the organic extract liquid salt water washing that merges, dry (MgSO 4) and evaporation, obtain resistates, at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, be eluent with it with the mixture of 50% ethyl acetate in isohexane, obtained required compound (4.6g).
1H?NMR?δ(CDCl 3)：1.3(d，3H)，3.4(s，3H)，3.5-3.6(m，2H)，4.5-4.6(m，1H)，6.65(s，1H)，6.95(d，1H)，7.05(s，1H)，7.1(s，1H)，7.25(d，1H).m/z?309(M+H) +
3-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-the 5-{[(2-aminomethyl phenyl) methyl] oxygen Base }-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D00661
To 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[(2-aminomethyl phenyl) methyl] the oxygen base } add oxalyl chloride (2.83mL) in the solution of phenylformic acid (9.55g) in DCM (140mL), the adding DMF that continues (1), and with mixture in stirring at room 16 hours.Remove DCM and excessive oxalyl chloride in the vacuum, remaining oily matter be dissolved among the DCM (25mL), be added in thiazolamine (2.84g) and the solution of triethylamine (7.88mL) in DCM (75mL) in 0-5 ℃, and with mixture in stirring at room 4 hours.Remove DCM and excessive triethylamine in the vacuum, and remaining oily matter is distributed between ethyl acetate (100mL) and 1M hydrochloric acid (100mL).Ethyl acetate layer is separated, use 1M hydrochloric acid, sodium bicarbonate aqueous solution and salt water washing successively, dry (MgSO 4) and evaporation, obtain resistates, at the enterprising circumstances in which people get things ready for a trip spectrometry of alumina purifying, be eluent with it with ethyl acetate, obtained required compound (11.0g). 1H?NMR?δ(CDCl 3)：1.3(d，3H)，2.35(s，3H)，3.4(s，3H)，3.5-3.6(m，2H)，4.55-4.6(m，1H)，5.0(s，2H)，6.8(s，1H)，6.95(d，1H)，7.15(s，1H)，7.25(m，5H)，7.4(d，1H).m/z?413(M+H) +
3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[(2-aminomethyl phenyl) methyl] the oxygen base } phenylformic acid
Figure A200810169286D00662
3-[{ (1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[(2-aminomethyl phenyl) methyl] the oxygen base } solution of methyl benzoate (10.65g) in THF (200mL) and methyl alcohol (50mL) is added in the solution of lithium hydroxide monohydrate (6.0g) in water (100mL).Mixture in stirring at room 16 hours, and is removed THF and methyl alcohol in a vacuum.Is to pH1 with hydrochloric acid with the water layer acidifying, and extracts with ethyl acetate (3 x 50mL).The organic extract salt water washing that merges, dry (MgSO 4), and evaporation, obtained required compound (9.55g).m/z?329(M-H) -
3-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[(2-aminomethyl phenyl) methyl] the oxygen base } Methyl benzoate
Figure A200810169286D00671
With the 3-hydroxyl-5-{[(2-aminomethyl phenyl that is stirring) methyl] the oxygen base } methyl benzoate (15.3g) and polymkeric substance prop up the suspension of triphenylphosphine (39.2g) in anhydrous DCM (900mL) that carries and cool off in ice bath, and drip diisopropyl azo-2-carboxylic acid (11.88mL).Reaction mixture was stirred 30 minutes in 0-5 ℃, and drip (R)-1-methoxyl group-propan-2-ol.Reaction mixture in stirring at room 16 hours, by diatomite filtration, and is evaporated DCM, obtain resistates, at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is eluent with the mixture of 10% ethyl acetate in isohexane with it, has obtained required compound (10.7g). 1H?NMR?δ(CDCl 3)：1.3(d，3H)，2.4(s，3H)，3.4(s，3H)，3.5-3.6(m，2H)，3.9(s，3H)，4.55-4.6(m，1H)，5.0(s，2H)，6.8(s，1H)，7.25(m，5H)，7.4(d，1H)
3-hydroxyl-5-{[(2-aminomethyl phenyl) methyl] the oxygen base } methyl benzoate
In 0 ℃ to 3, (50g, 0.30mol) add sodium hydride in the solution in DMF (500mL) (10.8g 0.27mol), and remains on reaction mixture below 10 ℃ the 5-methyl dihydroxy benzoate in batches.Reaction is heated to 15 ℃, and stirred 20 minutes.Mixture is cooled to 0 ℃, and with adding 2-methyl-benzyl bromine (36mL, 0.27mol) solution in DMF (50mL) in 30 minutes.Reaction is heated to room temperature and vacuum concentration, and remaining oily matter distributes between ethyl acetate (500mL) and water (250mL), ethyl acetate layer is separated water and salt water washing in succession, dry (MgSO 4) and evaporation, obtain resistates, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-100% ethyl acetate in isohexane, has been obtained required compound (21.9g). 1H?NMR?δ(CDCl 3)2.39(s，3H)，3.90(s，3H)，5.02(s，2H)，5.61(s，1H)，6.69(t，1H)，7.15-7.42(m，6H).
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[((1S)-1-methyl-2-{[three (1-methylethyl) silyl of use in embodiment 1c-1d synthetic] the oxygen base } ethyl) the oxygen base] preparation of benzamide is described below:
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[((1S)-1-methyl-2-{[three (1-methylethyl) silyl] the oxygen base } ethyl) the oxygen base] benzamide
Figure A200810169286D00681
Be added to 3-(benzyl oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-{ (1S)-1-methyl-2-[(triisopropyl silyl at following 10% palladium on carbon of argon gas) the oxygen base] oxyethyl group } (21.7g is 40.4mmol) in the mixture in anhydrous THF (480mL) for benzamide.With the reaction mixture degassing, place under the hydrogen capsule and stirred 16 hours.To substitute hydrogen with argon gas, and with mixture by diatomite filtration, then with the filtrate evaporation, and under high vacuum dry 1 hour, obtained this title compound (18.2g). 1H?NMR?δ(CDCl 3)：1.05(s，18H)，1.05-1.1(m，3H)，1.3(d，3H)，3.7(m，1H)，3.8(s，3H)，3.9(m，1H)，4.5(m，1H)，6.6(s，1H)，6.8(s，1H)，7.0(m，2H)，7.20(s，1H)，7.3(s，1H)，8.7(s，1H).m/z?448(M+H) +，446(M-H) -
3-(benzyl oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-{[1S)-1-methyl-2-[(triisopropyl silyl) oxygen base] oxyethyl group } benzamide
Figure A200810169286D00682
HATU (23.5g 61.8mmol) is added to 3-(benzyl oxygen base)-5-{ (1S)-1-methyl-2-[(triisopropyl silyl) oxygen base] oxyethyl group phenylformic acid (23.6g, 51.5mmol) in, the adding DMF (140mL) that continues, and be cooled to 0 ℃.Add 3-amino-1-methylpyrazole (6.00g.61.8mmol), the addings DIPEA (21.3mL) that continues, and will react on 0 ℃ of stirring 3 hours under argon gas.With solvent evaporation, resistates is dissolved in the ethyl acetate (500mL), and washs with citric acid solution (200mL), sodium hydrogen carbonate solution (150mL) and saturated brine solution (2 x 150mL).Also organic layer separation, dry (MgSO 4), filter and evaporation.By the column chromatography purifying, use the 1:4-1:1 ethyl acetate: the hexane wash-out, obtained this title compound, be colorless oil (21.7g).
1H?NMR?δ(CDCl 3)：1.05(s，18H)，1.05-1.1(m，3H)，1.3(d，3H)，3.7(m，1H)，3.8(s，3H)，3.9(m，1H)，4.5(m，1H)，5.1(s，2H)，6.7(s，1H)，6.8(s，1H)，7.0(m，2H)，7.1(s，1H)，7.3(s，1H)，7.35-7.5(m，5H)，8.5(s，1H).m/z538(M+H) +
3-(benzyl oxygen base)-5-{ (1S)-1-methyl-2-[(triisopropyl silyl) oxygen base] oxyethyl group } phenylformic acid
Figure A200810169286D00691
Lithium hydroxide monohydrate (12.14g, 0.289mol) solution in water (100mL) is added to 3-(benzyl oxygen base)-5-{ (1S)-1-methyl-2-[(triisopropyl silyl) the oxygen base] oxyethyl group methyl benzoate (62g, 0.131mol) in the solution in THF (300mL), and be heated to 43 ℃.To react and stir 16 hours, and remove THF in the vacuum, and the gained mixture is acidified to pH5 with the 10%w/v citric acid.It is extracted with ethyl acetate (2 x 300mL), and with the organic layer drying (MgSO that merges 4), filter and evaporation, obtained this title compound (60.2g).
1H?NMR?δ(CDCl 3)：1.05(s，18H)，1.05-1.1(m，3H)，1.35(d，3H)，3.7(m，1H)，3.9(m，1H)，4.5(m，1H)，5.1(s，2H)，6.8(s，1H)，7.3-7.5(m，7H).m/z?457(M-H) -
3-(benzyl oxygen base)-5-{ (1S)-1-methyl-2-[(triisopropyl silyl) oxygen base] oxyethyl group } methyl benzoate
Figure A200810169286D00701
In 0 ℃ (2R)-1-[(triisopropyl silyl) the oxygen base] propan-2-ol (56.1g, 242mmol) be added to 3-hydroxyl-5-{[phenyl methyl] the oxygen base } methyl benzoate (50g, 194mmol) and triphenylphosphine (63.5g, 242mmol) in the solution in anhydrous THF (500mL), continue under argon gas with added in 45 minutes DIAD (47.6mL, 242mmol).To be reflected at 0 ℃ and stir 1 hour, and be heated to room temperature 1 hour, then stirring at room 1 hour.With the THF evaporation, and the mixture of adding ethyl acetate (80mL) and hexane (120mL).This mixture was stirred 2 hours and filtered.The mixture of throw out with ethyl acetate (20mL) and hexane (180mL) washed, and filtrate is evaporated.Resistates column chromatography purifying, use the 1:20-1:10 ethyl acetate: the hexane wash-out has obtained this title compound (65.5g).
1H?NMR?δ(CDCl 3)：1.05(s，18H)，1.05-1.1(m，3H)，1.35(d，3H)，3.7(m，1H)，3.9(m，1H)，3.9(s，3H)，4.5(m，1H)，5.05(s，2H)，6.75(s，1H)，7.2(s，1H).7.3-7.5(m，6H).m/z?471(M-H) -
(2R)-and 1-[(triisopropyl silyl) the oxygen base] propan-2-ol
(83.8mL 390mmol) slowly was added to (2R)-the third-1 with 15 minutes, and (29.7g 390mmol) in the solution in DMF (100mL), remains on internal temperature below 15 ℃ the 2-glycol simultaneously the triisopropyl silyl chloride in 0 ℃.(66.4g 975mmol), is heated to room temperature with reaction mixture, and stirs 20 hours under argon gas to add imidazoles then.With 1M hydrochloric acid/ether (300mL/800mL) stopped reaction.Organic layer is separated, and wash with saturated brine solution then with 1M hydrochloric acid.With organic layer drying (MgSO 4), filter and evaporation.Carry out purifying by distilling down, obtained this title compound, be colorless oil (69.5g) at 10mmHg, 90-104 ℃. 1HNMR?δ(CDCl 3)：1.05(s，18H)，1.05-1.1(m，3H)，1.05(d，3H)，2.55(s，1H)，3.45(dd，1H)，3.7(dd，1H)，3.85(m，1H).
3-hydroxyl-5-{[phenyl methyl] the oxygen base } methyl benzoate
To stirring 3, add salt of wormwood (9mol) in the solution of 5-methyl dihydroxy benzoate (5.95mol) in DMF (6L), and this suspension stirred under argon gas in room temperature.In this mixture, slowly add bromotoluene (8.42mol) with 1 hour, have slight exotherm, and with reaction mixture in stirred overnight at room temperature.To react and use ammonium chloride solution (5L) water (35L) termination then modestly.This aqeous suspension extracts with DCM (1 x 3L and 2 x 5L).Extraction liquid water (10L) washing that merges, and with (MgSO 4) drying.Solution is evaporated in a vacuum, and divide crude product 3 batches to carry out chromatography purification (quick post, 3 x 2kg silicon-dioxide are used the gradient elution that is made of to the DCM that contains 50% ethyl acetate to pure DCM the hexane that contains 10% DCM) to remove real material just.Thick elutriant with the in batches further chromatography purification of 175g (Amicon HPLC, 5kg normal phase silicon-dioxide is with the isohexane wash-out that contains 20% v/v ethyl acetate), has been obtained required compound (productive rate 21%). 1H?NMR?δ(d 6-DMSO)：3.8(s，3H)，5.1(s，2H)，6.65(m，1H)，7.0(m，1H)，7.05(m，1H)，7.3-7.5(m，5H)，9.85(brs，1H).
Embodiment 2:3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00712
Salt of wormwood (0.181g, 1.31mmol) be added to 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (0.2g, 0.66mmol) and 5-(azetidine-1-base carbonyl)-2-chloropyridine (129mg, 0.66mmol) in the mixture of acetonitrile (5.0mL), and the mixture that will stir ' was heating 6 hours in 160 ℃ among the Biotage initiator Microwave '.Make mixture reach room temperature and normal pressure, and between ethyl acetate (50mL) and water (50mL), distribute.Ethyl acetate layer is separated, the washing of water (5 x 50mL) salt solution (50mL), dry (MgSO4) and evaporation, resistates is chromatogram purification on silicon-dioxide, with the mixture gradient elution of 0-5% methyl alcohol in ethyl acetate, obtained required compound (202mg). 1H NMR δ (CDCl 3): 1.33 (d, 3H), 2.38 (quin, 2H), 3.40 (s, 3H), 3.61-3.47 (m, 2H), 3.74 (s, 3H), 4.42-4.18 (m, 4H), 4.58 (sextet, 1H), 6.80 (d, 1H), 6.91 (t, 1H), 6.97 (d, 1H), 7.21 (t, 1H), 7.26 (d, 1H), 7.33 (t, 1H), 8.09-8.05 (m, 1H), 8.42 (d, 1H), 8.92 (s, 1H); M/z 466 (M+H) +
Adopt similar method, use suitable phenol and haloperidid, make embodiment 2a-2g:
Figure A200810169286D00721
Figure A200810169286D00731
The preparation of haloperidid is described among the embodiment 1.
The 3-hydroxyl that uses in embodiment 2 and 2a synthetic-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide is described below:
The 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00732
To 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] add the slurries of 10% palladium on carbon (727mg) in THF (1mL) and methyl alcohol (1mL) in the solution of benzamide (7.07g) in THF (50mL) and methyl alcohol (50mL).Mixture is placed under the vacuum, and under hydrogen, stirred 70 hours.Mixture is passed through diatomite filtration, and diatomite is washed with methyl alcohol (2 x 100mL), the evaporation in a vacuum that continues.And resistates is dissolved in the ethyl acetate (10mL), handles with isohexane (40mL), crosses the elimination solid, and with isohexane (50mL) washing, acquisition required compound (5.17g) uses it under situation about not being further purified.
1H?NMR?δ(d 6-DMSO)：1.22(d，3H)，3.28(s，3H，obscured?by?water)，3.38-3.53(m，2H)，3.76(s，3H)，4.65(m，1H)，6.44(m，1H)，6.54(m，1H)，6.93(s，1H)，7.04(s，1H)，7.57(m，1H)，9.63(brs，1H)，10.60(s，1H).m/z306(M+H) +，304(M-H) -
3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(benzene Ylmethyl) oxygen base] benzamide
Figure A200810169286D00741
3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } solution of phenylformic acid (8.73g) in DCM (150mL) is cooled to 0 ℃.Under agitation slowly add oxalyl chloride (4.81mL) and DMF (0.15mL).Mixture heating up to room temperature and stirred 16 hours, is removed organism in the vacuum then, and with resistates and toluene (75mL) azeotropic.Crude product is dissolved among the DCM (75mL), and slowly is added in the 3-amino-1-methylpyrazole (3.35g) and the suspension of DIPEA (14.4mL) in DCM (75mL) that is stirring.This mixture is removed organism in the vacuum then, and resistates is dissolved in the ethyl acetate (150mL) in stirring at room 18 hours.Organism is washed dry (MgSO with 1M aqueous hydrochloric acid (100mL) and salt solution (50mL) 4), evaporate in the vacuum then, obtain roughage.With it at 200g Biotage Flash 75 SiO 2Chromatogram purification on the post (with the mixture wash-out of 30-90% ethyl acetate in isohexane), and evaporate in the vacuum, required compound (7.07g) obtained.
1H?NMR?δ(d 6-DMSO)：1.23(d，3H)，3.28(s，3H，obscured?by?water)，3.40-3.52(m，2H)，3.77(s，3H)，4.70(m，1H)，5.03(s，2H)，6.56(m，1H)，6.71(m，1H)，7.18(s，1H)，7.24(s，1H)，7.32-7.47(brm，5H)，7.58(m，1H)，10.73(s，1H).m/z?396(M+H) +.
3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } phenylformic acid
Figure A200810169286D00742
With 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base solution of methyl benzoate (77.4mmol) in the mixture of THF (232mL) and methyl alcohol (232mL) handles with 2M sodium hydroxide solution (232mmol), and with reaction mixture in stirring at room 4 hours.
Gained solution with water (250mL) dilution, and remove most of organic solvent in a vacuum.Gained suspension washs with ether (3 x 200mL), and discards organic washes.Obtained aqueous solution is acidified to pH4 with the 2M hydrochloric acid soln, and extracts with ethyl acetate (2 x 200mL).Extraction liquid is merged, use the salt water washing, dry (MgSO 4), and evaporation, obtain required compound (99% yield).
1H?NMR?δ(d 6-DMSO)：1.20(d，3H)，3.46(m，2H)，4.64(m，1H)，5.15(s，2H)，6.83(app?t，1H)，7.06(s，1H)，7.13(s，1H)，7.30-7.49(m，5H)，12.67(br?s，1H)
3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } methyl benzoate
Figure A200810169286D00751
To 3-hydroxyl-5-{[phenyl methyl] the oxygen base add in the solution of methyl benzoate (77.4mmol) in THF polymkeric substance prop up the triphenylphosphine that carries (51.7g 3mmol/g charge capacity, 155mmol) and (R)-(-)-1-methoxyl group-2-propyl alcohol (102mmol).The solution that is stirring is covered with argon gas, and in ice bath, cool off.By syringe with dripping DIAD solution (116mmol) in 10 minutes.With solution stirring 20 minutes and filtration, resistates is washed with THF (500mL).And filtrate and washes merging, and evaporation, obtained required compound, it is used under situation about not being further purified.
1H?NMR?δ(d 6-DMSO)：3.26(s，3H)，3.44(m，2H)，3.82(s，3H)，4.63(m，1H)，5.14(s，2H)，6.85(s，1H)，7.05(s，1H)，7.11(s，1H)，7.30-7.47(m，5H)
1H NMR spectrum also comprises and a small amount of two (1-methylethyl) hydrazine-1, the signal of 2-dicarboxylic ester unanimity.
3-hydroxyl-5-{[phenyl methyl] the oxygen base } preparation of methyl benzoate is described in embodiment 1c.
3-hydroxyl-5-[(1-the methylethyl that in the preparation of embodiment 2b and 2c, uses) oxygen base]-N-1, the preparation of 3-thiazol-2-yl benzamide is described below:
3-hydroxyl-5-[(1-methylethyl) oxygen base]-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D00761
The 3-[(1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide (11.2g) is dissolved in the trifluoroacetic acid (60mL), and handles with thioanisole (17.8mL).Mixture in stirring at room 18 hours, is removed trifluoroacetic acid in the vacuum then.Resistates is handled with isohexane (100mL), and crosses the elimination solid, and then washs with isohexane (2 x 20mL).Solid is dissolved in the ethyl acetate (200mL), and washs with saturated sodium bicarbonate aqueous solution (100mL).Organism water (100mL) and salt solution (100mL) washing, and dry (MgSO4) are evaporated in the vacuum then, obtain solid, it is washed with isohexane (200mL), and dry in a vacuum, obtained required compound (7.18g). 1H?NMR?δ(d 6-DMSO)：1.27(d，6H)，4.55(m，1H)，6.49(m，1H)，7.02(s，1H)，7.14(s，1H)，7.25(d，1H)，7.54(d，1H)，9.73(s，1H)，12.44(s，1H).m/z279(M+H) +，277(M-H) -
The 3-[(1-methylethyl) oxygen base]-the 5-[(phenyl methyl) the oxygen base]-N-1,3-thiazol-2-yl benzamide
To the 3-[(1-methylethyl that is cooled to 0 ℃) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] under agitation slowly add oxalyl chloride (12.2mL) and DMF (0.4mL) in the solution of phenylformic acid (20g) in DCM (400mL).To room temperature, and restir 16 hours is removed organism in the vacuum then with mixture heating up, and with resistates and toluene (100mL) azeotropic.Roughage is dissolved among the DCM (200mL), and slowly is added in the thiazolamine (10.5g) and the suspension of diisopropyl ethyl amine (24.3mL) in DCM (200mL) that is stirring.Mixture in stirring at room 70 hours, is removed organism in the vacuum then.Resistates is dissolved in the ethyl acetate (300mL), and washs with 1M aqueous hydrochloric acid (300mL).Water layer is used ethyl acetate (300mL) extraction again, and the organism of merging washs with salt solution (75mL), and dry (MgSO 4), evaporation has in a vacuum obtained required compound (28g) then, and it is used under situation about not being further purified.
1H?NMR?δ(d 6-DMSO)：1.27(d，6H)，4.70(m，1H)，5.15(s，2H)，6.77(m，1H)，7.27(m，2H)，7.33-7.47(brm，6H)，7.55(d，1H).m/z?369(M+H) +，367(M-H) -；
1H NMR spectrum also contains the signal consistent with amount of ethyl acetate.
The 3-[(1-methylethyl) oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid
Figure A200810169286D00771
To the 3-[(1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (37g) is at THF: in the solution in the 1:1 mixture (300mL) of methyl alcohol, add 4M sodium hydroxide solution (150mL).Mixture was refluxed 45 minutes, remove organism in the vacuum then.The aqueous solution is acidified to pH4 with hydrochloric acid (2M), and uses ethyl acetate extraction.Organism is merged water and salt water washing, dry (MgSO 4) and vacuum in concentrate, obtained required compound (33.5g), it is used under situation about not being further purified.
1H?NMR?δ(d 6-DMSO)：1.26(d，6H)，4.59-4.69(m，1H)，5.15(s，2H)，6.80(appt，1H)，7.04(m，1H)，7.12(m，1H)，7.33(appt，1H)，7.40(t，2H)，7.46(d，2H)，12.95(s，1H)
The 3-[(1-methylethyl) oxygen base]-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D00772
To 3-hydroxyl-5-[(1-methylethyl) the oxygen base] add Anhydrous potassium carbonate (297mmol) and bromotoluene (143mmol) in the solution of methyl benzoate (25g) in DMF (250mL).Mixture was stirred 5 hours at 60 ℃, be cooled to room temperature then.Remove in the vacuum and desolvate, and resistates is distributed between ethyl acetate and water.Organism is merged, and water, salt water washing, dry (MgSO 4) and vacuum concentration, obtained required compound (37g), it is used under situation about not being further purified.
1H?NMR?δ(d 6-DMSO)：1.26(d，6H)，3.84(s，3H)，4.61-4.70(m，1H)，5.12(s，2H)，6.84(t，1H)，7.05(appt，1H)，7.12-7.15(m，1H)，7.31-7.37(m，1H)，7.40(t，2H)，7.46(d，2H)
3-hydroxyl-5-[(1-methylethyl) oxygen base] methyl benzoate
Figure A200810169286D00781
To stirring 3, add powder salt of wormwood (0.2mol) and 2-iodopropane (0.1mol) in the solution of 5-methyl dihydroxy benzoate (0.1mol) in DMF (180mL), and with the gained mixture in stirring at room 16 hours.Reaction mixture is poured in the water (1000mL), and with this mixture of extracted with diethyl ether.Extraction liquid is merged, and water (twice) and salt water washing successively; With solution drying (MgSO 4), filter and vacuum-evaporation, obtain crude product, be light yellow oil (12.6g).It is handled with toluene (40mL), and place and spend the night.Remove by filter undissolved material (initial phenol), and with filtrate vacuum-evaporation.Gained oily matter chromatography purification (2 x 90g Biotage silicon-dioxide tube) is with the hexane wash-out that contains ethyl acetate (10% increases to 15%v/v).Obtained this title compound, be oily matter (25% yield), differentiated that by tlc it is with identical by the sample of similar approach preparation. 1H?NMR?δ(d 6-DMSO)：1.2(d，6H)，3.8(s，3H)，4.5-4.6(hept，1H)，6.55(m，1H)，7.85(m，1H)，7.95(m，1H)，9.8(s，1H)
3-hydroxyl-5-[(1-the methylethyl that in the preparation of embodiment 2d and 2e, uses) oxygen base]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide is described below:
3-hydroxyl-5-[(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00782
The 3-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (51g; 0.14mol) be dissolved among methyl alcohol (500mL) and the THF (500mL), flask is vacuumized and with argon purge (3 times).Add 10% palladium on carbon (5.1g), flask is vacuumized and use at last hydrogen purge again.With reaction mixture in stirring at room 20 hours.Reaction mixture is vacuumized, and with nitrogen purging (3 times).Remove catalyzer by diatomite filtration, and with the filtrate vacuum concentration.Add ethyl acetate and filtration, obtained required compound (30.5g).Adopt identical method to obtain second batch of (second crop) material (4.0g).
1H?NMR?δ(d 6-DMSO)：1.30(d，6H)，3.78(s，3H)，4.68(sept，1H)，6.47(m，1H)，6.60(s，1H)，6.94(s，1H)，7.05(s，1H)，7.60(s，1H)，10.63(s，1H).m/z276(M+H) +
The 3-[(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] Benzamide
Figure A200810169286D00791
DMF (2) is added to the 3-[(1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (40.0g, 0.14mol) and oxalyl chloride (14.6mL is 0.17mol) in the solution in DCM (700mL).Mixture in stirring at room 4 hours, and is evaporated DCM and excessive oxalyl chloride in a vacuum.Remaining chloride of acid is dissolved among the DCM (300mL), and in 0 ℃ be added drop-wise to 1-methyl-3-amino-pyrazol (14.25g, 0.147mol) and triethylamine (41mL is 0.29mol) in the mixture in DCM (300mL).In stirring at room 24 hours.DCM is removed in evaporation in the vacuum, and resistates is distributed between ethyl acetate (400mL) and 1N hydrochloric acid (200mL).Ethyl acetate layer is used saturated sodium bicarbonate aqueous solution (200mL) and salt solution (100mL) washing, dry (MgSO in succession 4) and vacuum-evaporation.Resistates is chromatogram purification on silicon-dioxide, with the mixture gradient elution of 50% ethyl acetate in isohexane, has obtained required compound (51g). 1H?NMR?δ(CDCl 3)：1.30(d，6H)，3.61(s，3H)，4.50(sept，1H)，5.01(s，2H)，6.66(m，1H)，6.88(m，1H)，7.00(m，1H)，7.06(m，1H)，7.24(m，1H)，7.39(m，5H)，9.50(s，1H).m/z?366(M+H) +
The 3-[(1-methylethyl has above been described) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] benzoic preparation.
The 3-hydroxyl that in embodiment 2f and 2g, uses-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-preparation of N-(3-methyl isophthalic acid, 2,4-thiadiazoles-2-yl) benzamide is described below:
The 3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-2-yl) benzamide
Figure A200810169286D00801
With 3-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-5-{ phenyl methoxyl group)-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-2-yl) benzamide (9.53g) and the solution of thioanisole (13.9mL) in trifluoroacetic acid (45mL) were in stirring at room 16 hours.Remove trifluoroacetic acid in the vacuum, and remaining oily matter is distributed between ethyl acetate (100mL) and sodium bicarbonate aqueous solution (300mL).Water layer is separated, with ethyl acetate (2 x 100mL) extraction, and the organic extract liquid salt water washing that merges, dry (MgSO 4), and evaporation, obtain resistates, at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, the mixture of 50% ethyl acetate in isohexane is eluent, obtained required compound (4.5g) with it.
1H?NMR?δ(CDCl 3)：1.2(d，3H)，2.5(s，3H)，3.3(s，3H)，3.4-3.6(m，2H)，4.6-4.7(m，1H)，6.6(s，1H)，7.05(s，1H)，7.1(s，1H)，9.85(s，1H)，13.2(s，1H).m/z?324(M+H) +
3-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-5-{ phenyl methoxyl group }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-2-yl) benzamide
Figure A200810169286D00802
To 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } add oxalyl chloride (5.24mL) in the solution of phenylformic acid (15.8g) in DCM (260mL), the adding DMF (1drop) that continues, and with mixture stirring at room 16 hours.Remove DCM and excessive oxalyl chloride in the vacuum, remaining oily matter is dissolved among the DCM (50mL), and is added to 5-amino-3-methyl isophthalic acid, 2 in 0-5 ℃, in 4 thiadiazoles (6.05g) and the solution of triethylamine (14.6mL) in DCM (150mL), and with mixture stirring at room 16 hours.Remove DCM and excess of triethylamine in the vacuum, and remaining oily matter distributes between ethyl acetate (250mL) and 1M hydrochloric acid (150mL).Ethyl acetate layer is separated, use 1M hydrochloric acid, sodium bicarbonate aqueous solution and salt water washing successively, dry (MgSO 4), and evaporation acquisition resistates, with the enterprising circumstances in which people get things ready for a trip spectrometry of its alumina purifying, with ethyl acetate is eluent, at the enterprising circumstances in which people get things ready for a trip spectrometry of silicon-dioxide purifying, as eluent, obtained required compound (9.6g) then with the mixture of 30% ethyl acetate in isohexane.
1H?NMR?δ(CDCl 3)：1.3(d，3H)，2.45(s，3H)，3.4(s，3H)，3.5-3.6(m，2H)，4.55-4.6(m，1H)，5.05(s，2H)，6.8(s，1H)，7.1(m，2H)，7.25(m，5H)，10.7(s，1H).m/z?414(M+H) +
3-[(1S has above been described)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } benzoic synthetic
Embodiment 3:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00811
With 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (0.2g, 0.66mmol), 5-(azetidine-1-base carbonyl)-2-chloropyridine (158mg, 0.66mmol), cesium carbonate (640mg, 1.97mmol) and bromine three (triphenylphosphine) copper (I) (62mg, 0.07mmol) mixture in DMA (5mL) ' was stirring among the Biotage initiator Microwave ' 3 hours.Make mixture reach room temperature and normal pressure, and between ethyl acetate (50mL) and water (50mL), distribute.Ethyl acetate layer is separated water (5 x 50mL), salt solution (50mL) washing, dry (MgSO 4), and evaporation acquisition resistates, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-5% methyl alcohol in ethyl acetate, has been obtained required compound (113mg).With this compound partial crystallization from methyl alcohol.With this material partial crystallization from Virahol/isohexane or ethyl acetate/isohexane equally.132.7 ℃ of Mpt (fusing beginning).
1H NMR δ (CDCl 3): 1.32 (d, 3H), 2.36 (quin, 2H), 3.40 (s, 3H), 3.61-3.47 (m, 2H), 3.78 (s, 3H), 4.25 (t, 2H), 4.58 (sextet, 1H), 4.72 (t, 2H), 6.80 (t, 2H), 7.11 (s, 1H), 7.31-7.27 (m, 2H), 7.40-7.35 (m, 1H), 8.12 (d, 1H), 8.32 (s, 1H), 8.32 (s, 1H) .m/z 466 (M+H) +
Adopt similar approach to synthesize following compound by suitable phenol and bromopyridine:
Figure A200810169286D00821
Figure A200810169286D00831
The preparation of required bromopyridine has been described in the front.
3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base has been described in the front]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide, 3-hydroxyl-5-[(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide, 3-hydroxyl-5-[(1-methylethyl) oxygen base]-N-1,3-thiazol-2-yl benzamide, the 3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-N-1,3-thiazol-2-yl benzamide and 3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-2-yl) preparation of benzamide.
3-hydroxyl-5-[(1-the methylethyl that uses among the embodiment 3g is described below) the oxygen base]-preparation of N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide:
3-hydroxyl-5-[(1-methylethyl) oxygen base]-N-(3-methyl isophthalic acid 24-thiadiazoles-5-yl) benzamide
Figure A200810169286D00832
With the 3-[(1-methylethyl) the oxygen base]-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl)-5-[(phenyl methyl) the oxygen base] benzamide (33g, 86mmol), the solution of trifluoroacetic acid (160mL) and thioanisole (50.5mL) is in stirring at room 48 hours.Remove TFA in the vacuum, resistates is poured in the saturated sodium bicarbonate solution (300mL), and be extracted in the ethyl acetate (twice).With the organic extract liquid salt water washing that merges, dry (MgSO 4), drying, and remove in the vacuum and desolvate.Resistates is developed with DCM, and, obtained required compound (12.8g) with the mixture washing of 5% ethyl acetate in isohexane. 1H?NMR?δ(d 6-DMSO)：1.31(d，6H)，2.51(s，3H)，4.67(sept，1H)，6.58(s，1H)，7.08(s，1H)，7.24(s，1H)，9.88(s，1H)，13.25(brs，1H).m/z?294(M+H) +
The 3-[(1-methylethyl) oxygen base]-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D00841
DMF (2) is added to the 3-[(1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (29.6g, 0.103mol) and oxalyl chloride (10.78mL, 0.12mol) in the solution in DCM (500mL), mixture in stirring at room 4 hours, and is removed DCM and excessive oxalyl chloride in a vacuum.Remaining chloride of acid is dissolved among the DCM (220mL), and is added drop-wise to 5-amino-3-methyl isophthalic acid in 0 ℃, 2, the 4-thiadiazoles (12.43g, 0.108mol) and triethylamine (30.34mL is 0.216mol) in the mixture in DCM (220mL).Warm and reaction stirring at room 72 hours.Remove DCM in the vacuum, and resistates is distributed between ethyl acetate (400mL) and 1N hydrochloric acid (200mL).Ethyl acetate layer is used saturated sodium bicarbonate aqueous solution (200mL) and salt solution (100mL) washing, dry (MgSO successively 4) and concentrate in a vacuum.With the resistates chromatography purification,, obtained required compound (33g) with the mixture gradient elution wash-out of 20% ethyl acetate in isohexane.
1H?NMR?δ(CDCl 3)：1.32(d，6H)，2.31(s，3H)，4.51(sept，1H)，5.05(s，2H)，6.74(m，1H)，7.03(m，1H)，7.10(m，1H)，7.38(m，5H)，11.48(brs，1H).m/z?384(M+H) +
The 3-[(1-methylethyl has been described above) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] benzoic preparation.
Embodiment 4:3-{[5-(azetidine-1-base alkylsulfonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00842
Triethylamine (0.025mL, 0.18mmol) then triethyl silicane (0.96mL 6.03mmol) is added to acid chloride (II) (12mg) in the mixture in DCM (2mL), and stirred 15 minutes under argon gas.Drip 3-{[5-(azetidine-1-base alkylsulfonyl)-3-bromopyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (0.175g; 0.3mmol) mixture in DCM (2mL), and will react on stirring at room 24 hours.Add methyl alcohol, will react, and remove in the vacuum and desolvate by diatomite filtration.Add ethyl acetate (30mL), and with mixture water (30mL), citric acid (30mL), salt solution (30mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate.It at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-100% ethyl acetate in isohexane, has been obtained required compound (69mg).
1H NMR δ (CDCl 3): 1.26 (d, 3H), 2.09 (quin, 2H), 3.33 (s, 3H), 3.41-3.55 (m, 2H), 3.69 (s, 3H), 3.76 (t, 4H), 4.52 (sextet, 1H), 6.73 (d, 1H), 6.87 (t, 1H), 7.02 (d, 1H), 7.17 (t, 1H), 7.20 (m, 1H), 7.28 (s, 1H), 8.04 (m, 1H), 8.54 (d, 1H), 8.65 (s, 1H) .m/z 502 (M+H) +
3-{[5-(azetidine-1-base alkylsulfonyl)-3-bromopyridine-2-yl is described below] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide:
3-{[5-(azetidine-1-base alkylsulfonyl)-3-bromopyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00851
With 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (0.2g; 0.66mmol), 5-(azetidine-1-base alkylsulfonyl)-3-bromo-2-chloropyridine (204mg; 0.66mmol) and salt of wormwood (181mg, 1.31mmol) mixture in acetonitrile (5mL) ' was stirring 2 hours in 160 ℃ among the Biotage initiator Microwave '.Remove in the vacuum and desolvate, and resistates is dissolved in ethyl acetate and the water, organic layer is washed with salt solution (50mL), dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain brown oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture gradient elution of 50-100% ethyl acetate in isohexane, obtained required compound (175mg).
1H NMR δ (CDCl 3): 1.26 (d, 3H), 2.12 (quin, 2H), 3.33 (s, 3H), 3.54-3.40 (m, 2H), 3.68 (s, 3H), 3.79 (t, 4H), 4.52 (sextet, 1H), 6.73 (d, 1H), 6.89 (t, 1H), 7.18 (t, 1H), 7.21 (d, 1H), 7.30 (t, 1H), 8.27 (d, 1H), 8.38 (d, 1H), 8.77 (s, 1H) .m/z 580,582 (M+H) +
5-(azetidine-1-base alkylsulfonyl)-3-bromo-2-chloropyridine
Figure A200810169286D00861
(0.32g, (1g is 3.44mmol) in the solution in DCM (4mL) and pyridine (10mL), and stirring at room 22 hours 3.44mmol) to be added to 3-bromo-2-chloropyridine-5-alkylsulfonyl chlorine the azetidine hydrochloride.Remove in the vacuum and desolvate, and add ethyl acetate (30mL).Organic phase is washed dry (MgSO with 1M hydrochloric acid (20mL), water (20mL), salt solution (20mL) 4), remove in filtration and the vacuum and desolvate, obtained required compound (0.4g).
1H?NMR?δ(CDCl 3)：2.18(quin，2H)，3.83(t，4H)，8.26(s，1H)，8.66(s，1H).
Embodiment 5:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00862
DIPEA (0.28mL, 1.59mmol) be added to 5-[(3-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid (170mg, 0.4mmol), HATU (318mg, 0.84mmol) and azetidine hydrochloride (75mg, 0.8mmol) in the suspension in DMF (5mL), and with mixture in stirring at room 72 hours.Add ethyl acetate (40mL), and water (2 x 30mL), salt solution (30mL) washing, dry (MgSO 4), filter and vacuum concentration, obtain yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 0-100% ethyl acetate in isohexane, has been obtained required compound (48mg).
1H?NMR?δ(CDCl 3)：1.26(d，3H)，2.31(quin，2H)，3.32(s，3H)，3.41-3.55(m，2H)，3.71(s，3H)，4.19(t，2H)，4.52(m，1H)，4.60(t，2H)，6.72(s，1H)，6.86(m，1H)，7.16(m，1H)，7.20(m，1H)，7.28(m，1H)，8.25(s，1H)，8.52(s，1H)，8.52(s，1H).m/z?467(M+H) +
5-[(3-{[(1S is described below)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of pyrazine-2-formic acid:
5-[(3-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid
Figure A200810169286D00871
With 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (0.226g, 0.74mmol), 5-chloropyrazine-2-methyl-formiate (192mg, 1.11mmol) and salt of wormwood (205mg, 1.48mmol) mixture in acetonitrile (5mL) ' was stirring 4 hours in 160 ℃ among the Biotageinitiator Microwave '.Remove in the vacuum and desolvate, and add entry (30mL).The mixture acidifying, and be extracted in the ethyl acetate (3 x 50mL), the organism that merges is washed with salt solution (30mL), dry (MgSO 4), filter and vacuum concentration, obtain brown oil, it is the mixture of acid and methyl ester.Lithium hydroxide monohydrate (78mg, 1.85mmol) solution in water (2mL) be added to acid and ester (326mg, 0.74mmol) in the mixture in THF (4mL), and with mixture in stirring at room 20 hours.Remove THF in the vacuum, and aqueous residue is washed to remove impurity with ethyl acetate (20mL), use the acidifying of 1M citric acid then.This product is extracted in the ethyl acetate (2 x 100mL), and the organism that merges is washed with salt solution (50mL), dry (MgSO 4), filter and vacuum concentration, obtained yellow solid (0.17g).m/z?428(M+H) +
Embodiment 63-{[5-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00881
Cesium carbonate (488mg, 1.5mmol) be added to 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (187mg, 0.5mmol) and 5-(azetidine-1-base carbonyl)-2-chloro-1,3-thiazole (152mg, 0.75mmol) in the solution in acetonitrile (5mL), and the mixture that will stir in Biotage Initiator Microwave in 160 ℃ of heating 2 hours.Mixture is cooled to room temperature and normal pressure, pours in the water (75mL), use ethyl acetate (3 x 25mL) extraction then.The organic layer salt water washing that merges, dry (MgSO 4) and evaporation, obtain resistates, at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, eluent ethyl acetate has obtained required compound (142mg) with it. 1H NMR δ (CDCl 3): 1.3 (d, 6H), 2.4 (m, 2H), 3.7 (s, 3H), 4.1-4.4 (m, 4H), 4.5 (m, 1H), 6.75 (s, 1H), 6.9 (s, 1H), 7.2 (s, 1H), 7.25 (d, 1H), 7.4 (s, 1H) and 8.95 (s, 1H) .m/z 442 (M+H) +.
Following examples are to adopt similar method by 5-(azetidine-1-base carbonyl)-2-chloro-1,3-thiazoles and 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Figure A200810169286D00882
The preparation of 5-(azetidine-1-base carbonyl)-2-chloro-1,3-thiazoles is described below:
5-(azetidine-1-base carbonyl)-2-chloro-1,3-thiazoles
Under agitation (815mg, (0.53mL 6mmol), adds N then, N dimethyl formamide (1drop) 5mmol) slowly to add oxalyl chloride in the solution in methylene dichloride (10mL) to 2-diuril azoles-5-formic acid.Mixture was stirred 16 hours, remove organism in the vacuum then, and with resistates and toluene (100ml) azeotropic.Roughage is dissolved in the methylene dichloride (10mL), and slowly be added to the azetidine hydrochloride that stirring (560mg, 6mmol) and triethylamine (2.5mL is 18mmol) in the suspension in methylene dichloride (25mL).Mixture in stirring at room 2 hours, is removed organism then in a vacuum.Resistates is dissolved in ethyl acetate (50mL) and the water (25mL), organic layer is washed with salt solution (25mL), dry (MgSO 4) and evaporation, obtain resistates, at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, be eluent with it with the mixture of 40% ethyl acetate in isohexane, obtain 5-(azetidine-1-base carbonyl)-2-chloro-1,3-thiazoles quinoline. 1H NMR δ (CDCl 3): 2.4 (m, 2H), 4.1-4.4 (m, 4H), 4.55 (m, 1H), 7.2 (s, 1H) and 7.75 (s, 1H); M/z 203 (M+H) +.
3-hydroxyl-5-[(1-methylethyl has been described in the front) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Embodiment 7:3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00891
With 6-[(3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] Nicotinicum Acidum (0.20g, 0.378mmol), azetidine hydrochloride (0.106g, 0.114mmol), HATU (0.302g, 0.794mmol) and DIPEA (0.39mL, 0564mmol) solution in DMF (7mL) is in stirred overnight at room temperature.Add 3.5M hydrochloric acid (0.5mL), and with solution stirring 20 minutes.Solution is neutralized with saturated sodium bicarbonate solution.Add entry (20mL), and solution is extracted with ethyl acetate (50mL).With ethyl acetate layer salt water washing, dry (MgSO 4), and evaporation acquisition resistates, at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, the mixture wash-out of 50% ethyl acetate in isohexane obtains required product (27mg) with it. 1H NMR δ (CDCl 3): 1.32 (d, 3H), 2.38 (quintet, 2H), 3.77 (m, 2H), 3.80 (s, 3H), 4.29 (d, 4H), 4.57 (sextet, 1H), 6.81 (d, 1H), 6.91 (t, 1H), 6.99 (d, 1H), 7.27 (m, 2H), 7.38 (m, 1H), 8.42 (d, 1H), 8.86 (s, 1H) .m/z 452 (M+H) +
6-[(3-[((1S is described below)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of Nicotinicum Acidum:
6-[(3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-1-methyl second Base) oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] Nicotinicum Acidum
Figure A200810169286D00901
Lithium hydroxide monohydrate (0.10g, 4.17mmol) solution in water (15mL) is added to 6-[(3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] (0.90g is 1.67mmol) in the solution in THF (30mL) for the Nicotinicum Acidum methyl esters.With mixture in stirred overnight at room temperature.Remove THF in the vacuum, and gained solution is distributed between water (50mL) and ethyl acetate (75mL), then ethyl acetate layer is separated, with salt water washing and dry (MgSO4).By adding 1N hydrochloric acid (5.2mL) water layer is adjusted to pH7 then, and extracts with ethyl acetate (75mL).Ethyl acetate layer is separated, with salt water washing and dry (MgSO 4).Ethyl acetate layer is merged, and evaporation, required product (0.84g) obtained.
1H?NMR?δ(CDCl 3)：0.05(d，6H)，0.88(s，9H)，1.36(d，3H)，3.70-3.88(m，5H)，4.52-4.61(sex，1H)，6.98(d，2H)，7.01(d，1H)，6.28(s，1H)，7.44(s，1H)，7.57(s，1H)，8.04(m，1H)，8.72(s，1H)，10.62(s，1H).m/z?527(M+H) +
6-[(3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] the Nicotinicum Acidum methyl esters
Figure A200810169286D00911
With 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1.00g, 2.47mmol), 6-chlorine apellagrin methyl esters (0.450g, 2.60mmol) and cesium carbonate (1.204g, 3.71mmol) solution in acetonitrile (15mL) with microwave 160 ℃ the heating 90 minutes.Remove acetonitrile in the vacuum, and remaining oily matter is distributed between water (50mL) and ethyl acetate (75mL).Ethyl acetate layer is separated, use the salt water washing, dry (MgSO 4), and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (0.977g) with the mixture wash-out of 50% ethyl acetate in isohexane.
1H NMR δ (CDCl 3): 0.05 (d, 6H), 0.87 (s, 9H), 1.34 (d, 3H), 3.54 (m, 3H), 3.79 (s, 3H), 3.93 (s, 3H), 4.59 (sextet, 1H), 6.79 (d, 1H), 6.92 (t, 1H), 6.96 (s, 1H), 6.99 (s, 1H), 7.20 (t, 1H), 7.27 (d, 1H), 7.33 (t, 1H), 8.28 (d, 1H), 8.31 (d, 1H), 8.39 (s, 1H), 8.82 (d, 1H) .m/z (M+H) +541
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00912
3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (1.8g, 3.64mmol) be dissolved in the methyl alcohol (50mL), flask is vacuumized and with nitrogen purging (3 times).Add 10% palladium on carbon (0.2g), flask is vacuumized again, and use hydrogen purge at last.With reaction mixture till stirring at room 16 hours is finished to reaction.Reaction mixture is vacuumized, and with nitrogen purging (3 times).Cross the elimination catalyzer, and, obtained required compound (1.45g) the filtrate vacuum concentration. 1H?NMR?δ(d 6-DMSO)：0.02(d，6H)，0.83(s，9H)，1.18(d，3H)，3.66(m，2H)，3.72(s，3H)，4.51(m，1H)，6.42(m，1H)，6.52(m，1H)，6.90(s，1H)，7.02(s，1H)，7.55(m，1H)，9.58(brs，1H)，10.59(brs，1H).m/z?406(M+H) +
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D00921
DIPEA (4.06g, 23.4mmol) be added to 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (2.43g, 5.84mmol), 3-amino-1-methylpyrazole (0.85g, 8.76mmol) and HATU (4.66g, 12.3mmol) in the suspension in DMF (50mL), and in stirring at room 16 hours.With gained mixture partial concentration in a vacuum, pour in the water (100mL), and extract with ether (2 x 50mL).With extraction liquid water and salt water washing, dry then (MgSO 4), filter and be condensed into the opaque gum of partial crystallization.Crude product by the column chromatography purifying, with the mixture wash-out of 0-100% ethyl acetate in isohexane, has been obtained this title compound, be colorless oil (1.87g).
1H?NMR?δ(d 6-DMSO)：0.02(d，6H)，0.84(s，9H)，1.21(d，3H)，3.68(d，2H)，3.76(s，3H)，4.58(m，1H)，5.13(s，2H)，6.56(m，1H)，6.70(m，1H)，7.18(s，1H)，7.24(s，1H)，7.29-7.46(m，5H)，7.57(m，1H)，10.74(brs，1H).m/z?496(M+H) +
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) The oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid
Figure A200810169286D00922
3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (3.0g, 6.98mmol) be dissolved among the THF (50mL), and add entry (10mL) and lithium hydroxide monohydrate (586mg, 13.95mmol).The gained mixture under agitation in 45 ℃ of heating 2 hours, was placed 16 hours in room temperature then, and 45 ℃ of heating 4 hours.Add entry (40mL), and remove in a vacuum and desolvate.Gained solution is used 1M citric acid (2 equivalent) acidifying modestly, water and salt water washing, dry then (MgSO 4), filter also and evaporate in the vacuum, obtained this title compound, be colourless gum (2.58g).
1H?NMR?δ(d 6-DMSO)：0.02(d，6H)，0.84(s，9H)，1.17(d，3H)，3.66(m，2H)，4.43(m，1H)，5.05(s，2H)，6.56(brs，1H)，7.10(brs，1H)，7.17(brs，1H)，7.25-7.44(m，5H)，7.60(brs，1H).
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D00931
In 0 ℃ (2R)-1-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base } propan-2-ol (3.31g, 17.4mmol) be added to 3-hydroxyl-5-{[phenyl methyl] the oxygen base methyl benzoate (3.00g, 11.6mmol) in the solution in THF (50mL), (4.57g 17.4mmol), adds DIAD (3.43mL to the adding triphenylphosphine that continues then, 17.4mmol), reaction is heated to room temperature and stirred 16 hours.Water (100mL) and ether (400mL) stopped reaction, and with the organic layer separation, dry then (MgSO 4) and evaporation.By the column chromatography purifying, use the 1:15-1:5 ethyl acetate: the hexane wash-out, obtained this title compound, be colorless oil (4.00g, 80%).
1H?NMR?δ(CDCl 3)：0.03(s，3H)，0.05(s，3H)，0.89(s，9H)，1.29(d，3H)，3.63(dd，1H)，3.78(dd，1H)，3.92(s，3H)，4.44(m，1H)，5.08(s，2H)，6.77(m，1H)，7.40(m，7H)
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate.
(2R)-and 1-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base } propan-2-ol
Figure A200810169286D00932
(5.90g 39.5mmol) is added to (2R)-the third-1,2-glycol (3.00g the tertiary butyl (dimethyl) silyl chloride, 39.5mmol) in the solution in DCM (100mL), (7.10g 55.3mmol), and will be reflected under the argon gas and stir 72 hours the adding diisopropyl ethyl amine that continues.To react with ether (500mL) and water (140mL) dilution, and organic layer be separated dry then (MgSO 4), filter and evaporation.By the column chromatography purifying, use the 1:15-1:10 ethyl acetate: the hexane wash-out, obtained this title compound, be colorless oil (6.00g, 80%).
1H?NMR?δ(CDCl 3)：0.10(m，6H)，0.92(s，9H)，1.14(d，3H)，2.42(d，1H)，3.38(dd，1H)，3.60(dd，1H)，3.82(m，1H).
These data and document (J.Org.Chem., 1998, 53, 2300) and the middle data consistent of reporting.
Embodiment 8:3-{[3-chloro-5-(morpholine-4-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
With 5-chloro-6-[(3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] Nicotinicum Acidum (0.15g, 0.267mmol), morpholine (0.07mL, 0.803mmol), HATU (0.213g, 0.560mmol) and DIPEA (0.15mL, 0.861mmol) solution in DMF (7mL) was in stirring at room 2 days.Add entry (20mL), and solution is extracted with ethyl acetate (50mL).Ethyl acetate layer is separated, use the salt water washing, dry (MgSO 4), and evaporation, with resistates chromatogram purification on silicon-dioxide, with the mixture wash-out of 1% methyl alcohol in ethyl acetate.3.5M hydrochloric acid (0.5mL) is added to residual solid is dissolved in the solution in the methyl alcohol (5mL), and in stirring at room 20 minutes.This solution is neutralized with saturated sodium bicarbonate solution, remove methyl alcohol in a vacuum, and residual solution is distributed between water (20mL) and ethyl acetate.Ethyl acetate layer is separated, use the salt water washing, dry (MgSO 4) and evaporation, obtain required product (34mg). 1H NMR δ (d6-DMSO): 1.28 (d, 3H), 3.58 (m, 10H), 3.81 (s, 3H), 4.61 (sextet, 1H), 4.89 (t, 1H), 6.60 (d, 1H), 7.03 (t, 1H), 7.41 (s, 1H), 7.54 (s, 1H), 7.63 (d, 1H), 8.20 (q, 2H), 10.84 (s, 1H) .m/z 516 (M+H) +
5-chloro-6-[(3-[((1S is described below)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of Nicotinicum Acidum:
5-chloro-6-[(3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] Nicotinicum Acidum
Figure A200810169286D00951
To 5-chloro-6-[(3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyridine-3-carboxylic acid ester (1.2g, 2.09mmol) in the solution in THF (50mL), add lithium hydroxide monohydrate (0.219g, 5.22mmol) solution in water (30mL).Mixture at room temperature stirred spend the night.Remove THF in the vacuum, gained solution is distributed between water (50mL) and ethyl acetate (75mL), and ethyl acetate layer is separated, with salt water washing and dry (MgSO 4).Then water layer is adjusted to pH7 by adding 1N hydrochloric acid (5.2mL), and between ethyl acetate (75mL), distributes.Ethyl acetate layer is separated, with salt water washing and dry (MgSO 4).Ethyl acetate layer is merged, and evaporation, required product (1.1g) obtained.
1H NMR δ (d 6-DMSO): 0.01 (d, 6H), 0.82 (s, 9H), 1.22 (d, 3H), 3.73 (m, 2H), 3.76 (s, 3H), 4.55 (sextet, 1H), 6.55 (d, 1H), 6.90 (t, 1H), 7.30 (s, 1H), 7.44 (s, 1H), 7.58 (d, 1H), 8.36 (d, 1H), 8.19 (d, 1H), 8.51 (s, 1H) .m/z (M+H) +562
5-chloro-6-[(3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] the Nicotinicum Acidum methyl esters
Figure A200810169286D00952
With 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1.0g, 2.47mmol), 5,6-dichloro-nicotinic acid ethyl ester (0.57g, 2.59mmol) and salt of wormwood (0.855g, 6.14mmol) solution in acetonitrile (15mL) with microwave in 160 ℃ the heating 4 hours.Remove acetonitrile in the vacuum, and remaining oily matter distributes between water (50mL) and ethyl acetate (75mL).Ethyl acetate layer is separated, use the salt water washing, dry (MgSO 4), and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (1.1g) with the mixture wash-out of 50% ethyl acetate in hexane.Analytical data shows that some stage in the process that produces methyl ester, transesterify took place.
1H NMR δ (CDCl 3): 0.05 (d, 6H), 0.87 (s, 9H), 1.30 (d, 3H), 3.72 (s, 3H), 3.73 (m, 1H), 3.93 (s, 3H), 4.47 (sextet, 1H), 6.80 (d, 1H), 6.93 (t, 1H), 7.19 (t, 1H), 7.25 (d, 1H), 7.26 (s, 1H), 7.34 (t, 1H), 8.35 (d, 1H), 8.61 (d, 1H), 8.97 (s, 1H); M/z (M+H) +575
3-[((1S has been described in the front)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-preparation of 5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Embodiment 9:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00961
With 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (200mg, 0.64mmol), 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (164mg, 0.71mmol) and salt of wormwood (178mg, 1.28mmol) mixture in acetonitrile (5mL) ' was stirring 4 hours in 120 ℃ among the Biotage initiator Microwave '.Remove in the vacuum and desolvate, and resistates is distributed between ethyl acetate and water (100mL), organic layer washs with salt solution (50mL), dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain brown oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture gradient elution of 50-100% ethyl acetate in isohexane, obtained required compound (237mg).
1H NMR δ (CDCl 3): 2.39 (quintet, 2H), 3.74 (s, 3H), 4.25 (t, 2H), 4.37 (t, 2H), 4.63 (m, 2H), 4.74 (m, 3H); 6.81 (s, 1H), 7.01 (s, 1H), 7.29 (m, 1H), 7.31 (s, 1H), 7.42 (s, 1H), 8.17 (s, 1H), 8.23 (s, 1H), 9.26 (s, 1H) .m/z 506 (M+H) +
5-(azetidine-1-base carbonyl)-2 has been described above, the preparation of 3-dichloropyridine.
3-{[2-fluoro-1-(methyl fluoride) ethyl is described below] the oxygen base }-preparation of 5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide:
3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00971
With 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (2.46g, 6.13mmol) and the solution of 10% weight palladium on carbon (0.246g) in ethanol (100mL) under hydrogen in stirred overnight at room temperature.Solution is passed through
Figure A200810169286D0097183221QIETU
Filter, and with methyl alcohol (100mL) washing leaching cake.With solution evaporation, obtained required compound (1.78g). 1H?NMR?δ(d 6-DMSO)：3.78(s，3H)，4.72(m，4H)，4.97(m，1H)，6.57(d，2H)，7.03(s，1H)，7.16(s，1H)，7.59(s，1H).m/z?312(M+H) +
3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D00972
With 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (3.00g, 9.31mmol), 3-amino-1-methylpyrazole (1.83g, 18.6mmol), HATU (4.60g, 12.1mmol) and DIPEA (3.25mL, 18.6mmol) solution in DMF (12mL) is in stirred overnight at room temperature.Add entry (150mL), and solution is distributed with ethyl acetate (250mL).Ethyl acetate layer is separated, with salt water washing and dry (MgSO 4), and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtain required product (2.46g) with the mixture wash-out of 50% ethyl acetate in isohexane.
1H?NMR?δ(CDCl 3)：3.69(s，3H)，4.57(m，5H)，5.00(s，2H)，6.70(t，1H)，6.74(d，1H)，7.01(t，1H)，7.08(t，1H)，7.21(d，1H)，7.30(m，5H)，8.68(s，1H)；m/z?402(M+H) +
3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid
Figure A200810169286D00981
Lithium hydroxide monohydrate (2.32g, 55.1mmol) solution in water (100mL) is added to 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (7.41g, 22.0mmol) in the solution in THF (200mL), and with mixture in stirred overnight at room temperature.Remove THF in the vacuum, and gained solution is distributed between water (100mL) and ethyl acetate (250mL).Ethyl acetate layer is separated, with salt water washing and dry (MgSO 4).By adding 1M hydrochloric acid water layer is adjusted to pH7 then, and extracts with ethyl acetate (75mL).Ethyl acetate layer is separated, with salt water washing and dry (MgSO 4).Ethyl acetate layer is merged, and evaporation, required product (6.404g) obtained.
1H?NMR?δ(d 6-DMSO)：4.74(m，4H)，5.08(s，2H)，6.67(s，1H)，6.67(s，1H)，7.23(s，1H)，7.37(m，5H).m/z?231(M-H) -
3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D00982
Under rare gas element in 0 ℃ DIAD (7.63mL, 38.7mmol) be added drop-wise to 3-hydroxyl-5-{[phenyl methyl] the oxygen base methyl benzoate (5.00g, 19.4mmol), 1,3-difluoro propan-2-ol (3mL, 38.7mmol) and triphenylphosphine (10.16g is 38.7mmol) in the solution in THF (100mL).Make solution reach room temperature, and stirred 2 days.Remove THF in the vacuum, and with remaining oily matter with the mixture pulp of 20% ethyl acetate in isohexane.Stir after 90 minutes, mixture is filtered, and filtrate is evaporated.Remaining oily matter is used chromatography purification on silica gel,, obtained required compound (7.41g) with the mixture wash-out of 30% ethyl acetate in isohexane.
1H?NMR?δ(d 6-DMSO)：3.85(s，3H)，4.71(m，4H)，5.03(m，1H)，5.17(s，2H)，7.01(t，1H)，7.20(m，2H)，7.40(m，5H).m/z?335(M-H) -
3-hydroxyl-5-{[phenyl methyl has been described above] the oxygen base } preparation of methyl benzoate
Embodiment 10:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D00991
With 3-hydroxyl-5-{[(1S)-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (116mg, 0.4mmol) and 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (111mg, 0.48mmol) (111mg, solution 0.8mmol) under agitation heated 6 hours in 160 ℃ in microwave reactor for the salt of wormwood that contains in acetonitrile (2mL).Reaction mixture is filtered, and filtrate evaporated under reduced pressure is extremely done, and,, obtained required product (177mg) with the mixture wash-out of 50-100% ethyl acetate in hexane by the silicon-dioxide chromatography purification.
1H?NMR?δ(CDCl 3)：0.91(t，3H)，1.25(d，3H)，1.52-1.75(m，2H)，2.32(quin，2H)，3.72(s，3H)，4.17(t，2H)，4.25-4.34(m，3H)，6.73(d，1H)，6.81(t，1H)，7.12(s，1H)，7.21(d，1H)，7.24(s，1H)，8.09(d，1H)，8.18(d，1H)，8.54(s，1H)；m/z?484(M+H) +
5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine have been described in the front.
3-hydroxyl-5-{[(1S)-1-methyl-propyl is described below] the oxygen base }-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide:
The 3-hydroxyl-5-{[(1S)-the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
With 3-{[(1S)-the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] solution that contains 10% palladium on carbon of benzamide (900mg) in THF (5mL) and ethanol (5mL) stirs under hydrogen and spends the night.Remove by filter palladium on carbon, and, obtain required compound, be white solid (683mg) filtrate evaporated under reduced pressure. 1H?NMR?δ(CDCl 3)：0.95(t，3H)，1.27(d，3H)，1.54-1.80(m，2H)，3.79(s，3H)，4.31(q，1H)，6.57(t，1H)，6.81(d，1H)，6.96(s，1H)，6.98(s，1H)，7.30(s，1H)，7.57(s，1H)，8.84(s，1H)；m/z?290(M+H) +
3-{[(1S)-and the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D01002
HATU (1.19g, 3.13mmol) be added to 3-{[(1S)-the 1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (750mg, 2.5mmol) and 3-amino-1 methylpyrazole (291mg, 3mmol) in the mixture in DMF (5mL), adding DIPEA (the 0.807mL that continues, 6.25mmol), and will react and stir 16 hours.Reaction mixture is added in the ethyl acetate (30mL) water (10mL), 2N citric acid (10mL), saturated sodium bicarbonate aqueous solution (10mL) and salt solution (10mL) washing, dry then (MgSO 4), filter and be evaporated to the colloidality resistates.Resistates is dissolved in the mixture of 50% ether in ethyl acetate (50mL), with 2N hydrochloric acid (10mL), water (10mL), saturated sodium bicarbonate aqueous solution (10mL) and salt solution (10mL) washing, dry then (MgSO 4), filter and flash to foam (900mg), it is used under situation about not being further purified. 1H?NMR?δ(CDCl 3)：0.96(t，3H)，1.28(d，3H)，1.51-1.80(m，2H)，3.78(s，1H)，4.31(q，1H)，5.06(s，2H)，6.68(t，1H)，6.82(d，1H)，7.00(s，1H)，7.06(s，1H)，7.29(s，3H)，7.31-7.47(m，5H)，8.63(s，1H)；m/z380(M+H) +
3-{[(1S)-and the 1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid
Figure A200810169286D01011
With 3-{[(1S)-the 1-methyl-propyl] the oxygen base-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (14.87g, 47.36mmol) in the mixture of 1M aqueous sodium hydroxide solution (120mL) and THF in about 45 ℃ of vigorous stirring 4 hours.Most of THF is removed in decompression, and gained solution is distributed between water and ether.With aqueous phase as acidified, use ethyl acetate extraction with 2M hydrochloric acid then.With organism drying (MgSO 4) and concentrate in a vacuum, obtained white solid (11.5g). 1H?NMR?δ(CDCl 3)：0.90(t，3H)，1.20(d，3H)，1.49-1.69(m，2H)，4.33-4.46(m，1H)，5.12(s，2H)，6.75-6.79(m，1H)，6.99-7.03(m，1H)，7.06-7.11(m，1H)，7.26-7.47(m，5H)
3-{[(1S)-and the 1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D01012
DIAD (16.6mL, 84.0mmol) mixture in anhydrous THF (100mL) is added drop-wise to 3-hydroxyl-5-{[phenyl methyl that cold (ice bath) stirring] the oxygen base methyl benzoate (14.5g, 56.2mmol), R-(-)-sec-butyl alcohol (5g, 67.0mmol) and immobilized triphenylphosphine (28g, the 3mmol/g charge capacity, 84.0mmol) in the mixture in anhydrous THF (400mL), simultaneously temperature is remained on below 10 ℃.Mixture was stirred 3 hours, use ether (800mL) dilution and filtration then.Remove and desolvate, obtain canescence oily matter, it by the silica column chromatography purification, with the mixture wash-out of 25% ethyl acetate in hexane, is obtained required product (14.87g).
1H?NMR?δ(CDCl 3)：0.99(t，3H)，1.28(d，3H)，1.53-1.80(m，2H)，3.89(s，3H)，4.25-4.42(m，1H)，5.07(s，2H)，6.68-6.81(m，1H)，7.17-7.53(m，7H)；m/z?313(M-H) -
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate.
Embodiment 11:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-1- Methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01021
HATU (34mg, 0.89mmol) be added to 5-[(3-{[(1S)-the 1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid (190mg, 0.4mmol) and the azetidine hydrochloride (84mg is 0.89mmol) in the mixture in DMF (5mL).(0.311mL 1.79mmol), and will react and stir 16 hours to add DIPEA.Reaction mixture is added in the ethyl acetate (20mL) water (10mL), 2N citric acid (10mL), saturated sodium bicarbonate aqueous solution (10mL) and salt solution (10mL) washing, dry then (MgSO 4), filtration and reduction vaporization are to gummy residue.With resistates chromatogram purification on silicon-dioxide, with the mixture wash-out of 50-100% ethyl acetate in hexane, generation tree jelly, it is dissolved in the mixture of 50% ethyl acetate in ether, and water, salt solution (10mL) washed twice, dry then (MgSO 4), obtain required product, be foam (58mg).
1H?NMR?δ(CDCl 3)：0.91(t，3H)，1.25(d，3H)，1.51-1.75(m，2H)，2.31(quin，2H)，3.72(s，3H)，4.19(t，2H)，4.29(q，1H)，4.62(t，2H)，6.73(d，1H)，6.80(t，1H)，7.13(t，1H)，7.21(d，1H)，7.23(t，1H)，8.26(s，1H)，8.52(s，1H)，8.79(s，1H)；m/z?451(M+H) +
5-[(3-{[(1S is described below)-the 1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of pyrazine-2-formic acid:
5-[(3-{[(1S)-and the 1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid
With 3-hydroxyl-5-{[(1S)-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (116mg, 0.4mmol) and 5-chloropyrazine-2-methyl-formiate (83mg, 0.48mmol) in acetonitrile (2mL), contain salt of wormwood (111mg, 0.8mmol) solution, under agitation in 160 ℃ with microwave heating 6 hours.Analysis LC-MS shows formation acid but does not form ester.Reaction mixture is dissolved in the water (10mL), with the acidifying of 2N citric acid, and, uses the salt water washing, dry (MgSO with ethyl acetate (5 x 20mL) extraction 4) and reduction vaporization to doing, obtain required product (190mg), it is used under situation about not being further purified.
m/z?412(M+H) +
3-hydroxyl-5-{[(1S)-1-methyl-propyl has been described in the front] the oxygen base }-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Embodiment 12:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and the 1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01031
With 3-hydroxyl-5-{[(1S)-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (0.116g, 0.4mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (116mg, 0.48mmol), cesium carbonate (392mg, 1.2mmol) and bromine three (triphenylphosphine) copper (I) (38mg, 0.04mmol) mixture in DMA (5mL) stirred 4 hours in 160 ℃ in microwave reactor.Product is dissolved in ethyl acetate (20mL) and the water (15mL), and each layer separated.Organic layer is washed dry (MgSO with salt solution (50mL) 4), remove in filtration and the vacuum and desolvate, obtain brown oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-5% methyl alcohol in ethyl acetate, and then is loaded on the silica column, with the mixture gradient elution of 0-5% methyl alcohol in DCM, obtained required compound, be white foam shape thing, its crystallization when placing.Material with the mixture development of 50% ethyl acetate in hexane, has been obtained required product, be white solid (95mg). 1H?NMR?δ(CDCl 3)：0.90(t，3H)，1.23(d，3H)，1.51-1.72(m，2H)，2.28(quin，2H)，3.73(s，3H)，4.18(t，2H)，4.28(q，1H)，4.64(t，2H)，6.67(t，1H)，6.72(d，1H)，6.99(s，1H)，7.15(s，1H)，7.22(d，1H)，7.31(d，1H)，8.04(d，1H)，8.26(s，1H)，8.41(s，1H)；m/z?450(M+H) +
3-hydroxyl-5-{[(1S)-1-methyl-propyl has been described in the front] the oxygen base }-description of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Embodiment 13:3-{[2-(azetidine-1-base carbonyl) pyrimidine-5-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01041
With 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (250mg, 0.91mmol), 2-(azetidine-1-base carbonyl)-5-bromo pyrimi piperidine (242mg, 1.0mmol), cesium carbonate (886mg, 2.72mmol) and bromine three (triphenylphosphine) copper (I) (423mg, 0.45mmol) mixture in DMA (5mL) ' was stirring 4 hours in 160 ℃ among the Biotage initiator Microwave '.Mixture is added in ethyl acetate (50mL) and the water (50mL), organic layer is suitable with salt solution (50mL), dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain brown oil.With resistates at SO 2The last chromatography purification of using with the mixture gradient elution of 0-10% methyl alcohol in ethyl acetate, has obtained required compound (199mg).
1H?NMR?δ(CDCl 3)：1.39(d，6H)，2.40(quin，2H)，3.82(s，3H)，4.35(t，2H)，4.62(mult，1H)，4.68(t，2H)，6.79(s，1H)，6.84(s，1H)，7.20(s，1H)，7.33(mult，2H)，8.60(s，2H)，8.97(s，1H)；m/z?437(M+H) +
Adopt similar method by 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the synthetic following compound of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Figure A200810169286D01042
3-hydroxyl-5-[(1-methylethyl has been described in the front) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
The preparation of 2-(azetidine-1-base carbonyl)-5-bromo pyrimi piperidine is described below:
2-(azetidine-1-base carbonyl)-5-bromo pyrimi piperidine
Figure A200810169286D01051
(1.50mL, (2.86g is 14.0mmol) in the mixture in DCM (40mL) 16.8mmol) then DMF (2) to be added to 5-bromo pyrimi piperidine-2-formic acid (according to the literature method preparation of describing among the WO 2005/028452) oxalyl chloride.Reaction mixture in stirring at room 2 hours, is removed volatile matter, and resistates is dissolved among the DCM (40mL) in the vacuum.Add the azetidine hydrochloride (1.44g, 15.4mmol), the adding triethylamine that continues (4.29mL, 30.8mmol), and with mixture in stirring at room 72 hours.Mixture is concentrated in a vacuum, and ethyl acetate (100mL) is added in the resistates.With organism water (100mL), citric acid solution (50mL), saturated sodium bicarbonate solution (50mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain yellow solid, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture gradient elution of 50-100% ethyl acetate in isohexane, obtained required compound (0.86g).1HNMR?δ(CDCl 3)：2.39(quin，2H)，4.32(t，2H)，4.63(t，2H)，8.92(s，2H)；m/z?242，244(M+H) +
Embodiment 14:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
With 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (100mg, 0.32mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (86mg, 0.35mmol), cesium carbonate (209mg, 0.64mmol) and bromine three (triphenylphosphine) copper (I) (150mg, 0.16mmol) mixture in DMA (5mL) ' was stirring 3 hours in 160 ℃ among the Biotage initiator Microwave '.Reaction mixture is added in ethyl acetate (50mL) and the water (50mL), organic layer is washed with salt solution (50mL), dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain brown oil.With resistates SiO 2Chromatography purification, with the mixture wash-out of 0-100% ethyl acetate in isohexane, obtain colorless oil, its seemingly product and eliminate product (3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[1-(methyl fluoride) vinyl] the oxygen base-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide) mixture.This mixture is dissolved in the chloroform (5mL), adds TFA (0.5mL) and mixture was stirred 2 hours.Observe the degraded fully of eliminating product by LCMS, and, add entry then the mixture vacuum concentration.With the mixture neutralization, and be extracted in the ethyl acetate (2 x 30mL), with salt solution (30mL) washing, dry (MgSO 4), filter and concentrate, obtain yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 0-100% ethyl acetate in isohexane, has been obtained required compound (13mg).
1H?NMR?δ(CDCl 3)：2.29(quin，2H)，3.76(s，3H)，4.19(t，2H)，4.55(mult，2H)，4.67(mult，5H)，6.72(s，1H)，6.80(mult，1H)，7.09(s，1H)，7.25(mult，2H)，7.32(mult，1H)，8.06(d，1H)，8.26(d，1H)，8.43(s，1H)；m/z472(M+H) +
3-{[2-fluoro-1-(methyl fluoride) ethyl has been described in the front] the oxygen base }-preparation of 5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Embodiment 15:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01061
DIPEA (0.45mL, 2.58mmol) be added to 5-[(3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid (0.28g, 0.65mmol), azetidine hydrochloride (121mg, 1.29mmol) and HATU (516mg, 1.36mmol) in the mixture in DMF (5mL), and in stirring at room 72 hours.Add ethyl acetate (40mL), and with organism water (2 x 30mL), salt solution (30mL) washing, dry (MgSO4), filter and vacuum concentration, obtain yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 0-100% ethyl acetate in isohexane, has been obtained required compound (109mg). 1H?NMR?δ(CDCl 3)：2.32(quin，2H)，3.70(s，3H)，4.19(t，2H)，4.54-4.72(m，7H)，6.73(s，1H)，6.93(t，1H)，7.22(d，1H)，7.25(s，1H)，7.34(s，1H)，8.28(d，1H)，8.78(d，1H)，8.80(s，1H)；m/z?473(M+H) +
Adopt similar method by the 5-[(3-[(1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] the synthetic following compound of pyrazine-2-formic acid.
Figure A200810169286D01071
5-[(3-{[2-fluoro-1-(methyl fluoride) ethyl is described below] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of pyrazine-2-formic acid:
5-[(3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid
Figure A200810169286D01072
Lithium hydroxide monohydrate (77mg, 1.82mmol) solution in water (2mL) is added to 5-[(3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-methyl-formiate (325mg, 0.73mmol) in the mixture in THF (4mL), and with mixture in stirring at room 20 hours.Remove THF in the vacuum, and aqueous residue is washed to remove impurity with ethyl acetate, use the acidifying of 1M citric acid then.Add ethyl acetate, by removing by filter white solid, and dry in a vacuum, obtained required compound, be white solid (0.28g). 1H?NMR?δ(d 6-DMSO)：3.79(s，3H)，4.77(m，4H)，5.12(t，1H)，6.59(s，1H)，7.22(s，1H)，7.50(s，1H)，7.62(s，2H)，8.69(s，1H)，8.80(s，1H)，10.90(s，1H)，13.50(s，1H)，m/z?434(M+H) +
Adopt similar method, by the 5-[(3-[(1-methylethyl) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-methyl-formiate, make the 5-[(3-[(1-methylethyl that in the preparation of embodiment 15a, uses) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid.
Figure A200810169286D01081
5-[(3-{[2-fluoro-1-(methyl fluoride) ethyl is described below] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of pyrazine-2-methyl-formiate:
5-[(3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-methyl-formiate
Figure A200810169286D01082
With 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (250mg, 0.8mmol), 5-chloropyrazine-2-carboxylic acid methyl ester (208mg, 1.20mmol) and salt of wormwood (222mg, 1.61mmol) mixture in acetonitrile (5mL) ' was stirring 3 hours in 120 ℃ among the Biotageinitiator Microwave '.Remove in the vacuum and desolvate, and resistates is dissolved in ethyl acetate (50mL) and the water (50mL), organic layer is washed with salt solution (50mL), dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture wash-out of 0-100% ethyl acetate in isohexane, obtained required compound (0.325g). 1H?NMR?δ(CDCl 3)：3.71(s，3H)，3.96(s，3H)，4.56(m，2H)，4.68(m，3H)，6.73(s，1H)，6.95(s，1H)，7.22(s，1H)，7.27(s，1H)，7.36(s，1H)，8.46(s，1H)，8.67(s，1H)，8.67(s，1H)；m/z?448(M+H) +
Adopt similar method, by 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide, make the 5-[(3-[(1-methylethyl that is used for embodiment 15a) the oxygen base]-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-methyl-formiate.
Figure A200810169286D01091
3-{[2-fluoro-1-(methyl fluoride) ethyl has been described in the front] the oxygen base }-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Embodiment 16:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01092
With 3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (110mg, 0.32mmol), 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (75mg, 0.32mmol) and salt of wormwood (89mg, 0.64mmol) mixture in acetonitrile (5mL) ' was stirring 3 hours in 160 ℃ among the Biotage initiator Microwave '.Remove in the vacuum and desolvate, (50mL) is added in the resistates ethyl acetate, with its water (20mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain yellow oil.With resistates SiO 2Chromatography purification with the mixture gradient elution of 50-100% ethyl acetate in isohexane, has obtained required compound (92mg). 1H NMR δ (CDCl 3): 1.40 (d, 3H), 2.41 (quin, 2H), 3.81 (s, 3H), 4.01 (mult, 2H), 4.26 (t, 2H), 4.38 (t, 2H), 4.67 (sextet, 1H), 6.29 (t, 1H), 6.82 (d, 1H), 6.96 (t, 1H), 7.27 (t, 1H), 7.31 (d, 1H), 7.38 (t, 1H), 8.18 (d, 1H), 8.26 (d, 1H), 8.77 (s, 1H), m/z 536 (M+H) +
Adopt similar method by 3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 5-(azetidine-1-base carbonyl)-2-chloropyridine make following compound:
Figure A200810169286D01101
5-(azetidine-1-base carbonyl)-2 has been described in the front, the preparation of 3-dichloropyridine and 5-(azetidine-1-base carbonyl)-2-chloropyridine.
3-({ (1S)-2-[(difluoromethyl) oxygen base is described below]-the 1-methylethyl } the oxygen base)-preparation of 5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide:
3-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01102
3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (0.1g, 0.23mmol) be dissolved among ethanol (3mL) and the THF (3mL), flask is vacuumized, and with argon purge (3 times).Add 10% palladium on carbon (0.01g), flask is vacuumized and uses at last hydrogen purge again.With reaction mixture till stirring at room 20 hours is finished to reaction.Reaction mixture is vacuumized, and with nitrogen purging (3 times).Remove catalyzer by diatomite filtration, and the filtrate vacuum concentration has been obtained required compound (70mg). 1H NMR δ (CDCl 3): 1.28 (d, 3H), 3.71 (s, 3H), 3.80-3.95 (m, 2H), 4.51 (sextet, 1H), 5.96-6.36 (t, 1H), 6.53 (s, 1H), 6.73 (s, 1H), 6.91 (s, 1H), 6.96 (s, 1H), 7.22 (s, 1H), 8.83 (s, 1H) .m/z 342 (M+H) +.
3-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazoles -3-yl)-and the 5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D01111
DIPEA (0.198mL, 1.14mmol) be added to 3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (0.1g, 0.28mmol), 3-amino-1-methylpyrazole (39mg, 0.4mmol) and HATU (0.227g, 0.6mmol) in the mixture in DMF (3mL), and in stirring at room 20 hours.Add ethyl acetate (30mL), and with mixture water (30mL), salt solution (30mL) washing, dry (MgSO 4), filter and vacuum concentration, obtain yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-100% ethyl acetate in isohexane, has been obtained required compound (0.1g).
1H?NMR?δ(CDCl 3)：1.36(d，3H)，3.68(s，3H)，3.82-3.95(m，2H)，4.48(sex，1H)，5.00(s，2H)，6.19(t，1H)，6.63(s，1H)，6.73(s，1H)，6.93(s，1H)，7.03(s，1H)，7.28(m，1H)，7.35(m，5H)，8.59(s，1H).m/z432(M+H) +.
3-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] phenylformic acid
Figure A200810169286D01112
Lithium hydroxide monohydrate (19mg, 0.45mmol) solution in water (2mL) is added to 3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (0.11g, 0.3mmol) in the mixture in THF (4mL), and with mixture in stirring at room 20 hours.Remove THF in the vacuum, and water layer is adjusted to pH3 with citric acid, be extracted into then in the ethyl acetate (2 x 30mL).With organic layer water (30mL), salt solution (30mL) washing, dry (MgSO4) removes in filtration and the vacuum and desolvates, and obtained required compound (0.1g).
1H NMR δ (d 6-DMSO): 1.27 (d, 3H), 4.00 (m, 2H), 4.75 (septet, 1H), 5.15 (s, 2H), 6.72 (t, 1H), 7.08 (t, 1H), 7.16 (t, 1H), 7.41 (m, 5H), 12.95 (s, 1H) .m/z 351 (M+H) +.
3-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D01121
With 2-(fluorosulfonyl) difluoroacetic acid (0.239mL; 2.31mmol) under agitation in 45 ℃ of 3-{[(1S that are added drop-wise to the degassing)-2-hydroxyl-1-methylethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (0.73g; 2.31mmol) and cupric iodide (I) (88mg is 0.46mmol) in the mixture in acetonitrile (10mL).To react on 45 ℃ stirred 24 hours.Remove in the vacuum and desolvate, and add ethyl acetate (30mL).With organism water (30mL), salt solution (30mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-30% ethyl acetate in isohexane, has been obtained required compound (0.11g).
1H NMR δ (CDCl 3): 1.37 (d, 3H), 3.93 (s, 3H), 4.00 (m, 2H), 4.63 (septet, 1H), 5.10 (s, 2H), 6.28 (t, 1H), 6.77 (t, 1H), 7.28 (t, 1H), 7.41 (m, 6H) .m/z 367 (M+H) +.
3-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D01122
Bromotoluene (1.89g, 7.20mmol) be added to 3-hydroxyl-5-[(1S)-2-hydroxyl-1-methyl ethoxy] methyl benzoate (1.55g, 6.86mmol) and salt of wormwood (1.89g 0.014mol) in the mixture in DMF (16mL), and will react on stirring at room 20 hours.Add ethyl acetate (40mL), and with mixture water (40mL), saturated sodium bicarbonate solution (40mL), salt solution (40mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain red oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture gradient elution of 0-100% ethyl acetate in isohexane, obtained required compound (1.7g).
1H?NMR?δ(CDCl 3)：1.30(d，3H)，1.95(m，1H)，3.76(m，2H)，3.92(s，3H)，4.53(m，1H)，5.11(s，2H)，6.78(t，1H)，7.25(m，1H)，7.32(m，1H)，7.45(m，5H).m/z?317(M+H) +.
The 3-hydroxyl-5-[(1S)-2-hydroxyl-1-methyl ethoxy] methyl benzoate
Figure A200810169286D01131
Trimethyl silyl iodine (115mL 0.79mol) is added to 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base] (38.01g 0.158mol) in the solution in acetonitrile (500mL), and stirred 24 hours methyl benzoate.Add methyl alcohol (300mL), and will react and stir 10 minutes.The 10% w/v Sulfothiorine pentahydrate aqueous solution (100mL) is added in this mixture, and stirred 20 minutes.Reaction mixture is neutralized with saturated sodium bicarbonate aqueous solution, remove organic solvent in the vacuum, and product is extracted in the ethyl acetate (4 x 100mL).With the organic layer drying (MgSO that merges 4), remove in filtration and the vacuum and desolvate.With roughage crystallization from ethyl acetate, obtained this title compound (16.80g).
1H?NMR?δ(d 6-DMSO)：1.18(d，3H)，3.40-3.55(m，2H)，3.80(s，3H)，4.35(sex，1H)，4.80(t，1H)，6.57(m，1H)，6.90(m，2H)，9.75(s，1H).m/z304(M+H) +
The 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base] methyl benzoate
3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } (50.0g 0.152mmol) is dissolved in THF to methyl benzoate: in ethanol (600mL) mixture, flask vacuumized and with nitrogen purging (3 times).Add 10% palladium on carbon (5.0g), flask is vacuumized again, and use hydrogen purge at last.With reaction mixture till stirring at room 20 hours is finished to reaction.Reaction mixture is vacuumized, and with nitrogen purging (3 times).Cross the elimination catalyzer, and the filtrate vacuum concentration obtained required compound (36.7g).
1H?NMR?δ(d 6-DMSO)：1.2(d，3H)，3.25(s，3H)，3.44(m，2H)，3.82(s，3H)，4.55(m，1H)，6.6(s，1H)，6.9(s，1H)，6.95(s，1H)，9.8(s，1H).
3-[(1S has been described in the front)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } preparation of methyl benzoate.
Embodiment 17:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01141
With 3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (220mg, 0.64mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (171mg, 0.71mmol), cesium carbonate (419mg, 1.29mmol) and bromine three (triphenylphosphine) copper (I) (300mg, 0.32mmol) mixture in DMA (5mL) ' was stirring 4 hours in 160 ℃ among the Biotageinitiator Microwave '.Reaction mixture is added in ethyl acetate (50mL) and the water (50mL), and organic layer is washed with salt solution (50mL), dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain brown oil.With resistates SiO 2Chromatography purification with the mixture wash-out of 0-100% ethyl acetate in isohexane, has obtained required compound (102mg).
1H NMR δ (CDCl 3): 1.28 (d, 3H), 2.28 (quin, 2H), 3.68 (s, 3H), 3.90 (mult, 2H), 4.17 (t, 2H), 4.54 (sextet, 1H), 4.63 (t, 2H), 6.19 (t, 1H), 6.71 (t, 1H), 6.73 (s, 1H), 7.06 (s, 1H), 7.22 (mult, 2H), 7.30 (mult, 1H), 8.04 (d, 1H), 8.24 (d, 1H), 8.84 (s, 1H); M/z 502 (M+H) +
3-({ (1S)-2-[(difluoromethyl) oxygen base has been described in the front]-the 1-methylethyl } the oxygen base)-preparation of 5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Embodiment 18:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazoles -3-yl) benzamide
Figure A200810169286D01151
DIPEA (0.41mL, 2.33mmol) be added to 5-[(3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid (0.27g, 0.58mmol), azetidine hydrochloride (109mg, 1.17mmol) and HATU (466mg, 1.22mmol) in the mixture in DMF (5mL), and in stirring at room 24 hours.Add ethyl acetate (40mL), and with organism water (2 x 30mL), salt solution (30mL) washing, dry (MgSO 4), filter and vacuum concentration, obtain yellow oil.With resistates SiO 2Chromatography purification with the mixture wash-out of 0-5% methyl alcohol in ethyl acetate, has obtained required compound (124mg).
1H NMR δ (CDCl 3): 1.28 (d, 3H), 2.30 (quin, 2H), 3.65 (s, 3H), 3.90 (m, 2H), 4.18 (t, 2H), 4.53 (sextet, 1H), 4.61 (t, 2H), 6.19 (t, 1H), 6.73 (d, 1H), 6.84 (t, 1H), 7.18 (m, 2H), 7.28 (s, 1H), 8.25 (s, 1H), 8.76 (s, 1H), 9.14 (s, 1H); M/z 503 (M+H) +.
5-[(3-({ (1S)-2-[(difluoromethyl) oxygen base is described below]-the 1-methylethyl } the oxygen base)-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of pyrazine-2-formic acid:
5-[(3-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid
Figure A200810169286D01152
With 3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (220mg, 0.64mmol), 5-chloropyrazine-2-methyl-formiate (112mg, 0.64mmol) and salt of wormwood (178mg, 1.29mmol) mixture in acetonitrile (5mL) ' was stirring 4 hours in 160 ℃ among the Biotage initiator Microwave '.Remove in the vacuum and desolvate, and add entry (20mL).Mixture is adjusted to pH3 with the 1M citric acid, and is extracted in the ethyl acetate (2x 50mL).With organism water (20mL), salt solution (50mL) washing that merges, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain yellow oil (0.19g), mixture of its acid seemingly and methyl ester.Lithium hydroxide monohydrate (26mg, 0.60mmol) solution in water (3mL) be added to acid and ester (0.19g, 0.4mmol) in the mixture in THF (6mL), and with mixture in stirring at room 72 hours.Remove organism in the vacuum, and resistates is adjusted to pH3 with the 1M citric acid, extracting twice is in ethyl acetate, and with the organism merging, water (30mL), salt solution (30mL) wash, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained required compound, be yellow solid (0.17g).
m/z?464(M+H) +
3-({ (1S)-2-[(difluoromethyl) oxygen base has been described in the front]-the 1-methylethyl } the oxygen base)-preparation of 5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Embodiment 19:3-{ (5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1:1)
Figure A200810169286D01161
With 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (95mg, 0.44mmol), 3-hydroxyl-5-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-mixture (129mg of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1:1), 0.42mmol) and salt of wormwood (146mg, 1.05mmol) solution in acetonitrile (3mL) in microwave reactor in 160 ℃ the heating 5 hours.Remove acetonitrile in the vacuum, and resistates is dissolved in the ethyl acetate (25mL).With organic solution water (25mL), salt solution (25mL) washing, dry (MgSO 4) and be evaporated to resistates, with its chromatography purification on alumina,, obtained required product (56mg) with the mixture gradient elution of 0-5% methyl alcohol in ethyl acetate.
1H?NMR?δ(CDCl 3)：1.18(m，6H)，2.32(m，3H)，3.66(s，3H)，3.79(quin，1H)，4.15(m，3H)，4.28(t，2H)，6.72(d，1H)，6.85(t，1H)，7.17(s，1H)，7.19(s，1H)，7.29(s，1H)，8.08(m，1H)，8.15(m，1H)，9.10(s，1H)；m/z501(M+H) +
This non-enantiomer mixture is prepared HPLC by chirality on Chiralpak IA (250mm x 20mm) No.EG014 post separate, mixture wash-out with isohexane/ethyl acetate/acetate/triethylamine (40/60/0.2/0.1), obtain the isomer (43mg) of first wash-out, embodiment 19a, with the isomer (40mg) of second wash-out, embodiment 19b.
5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine have been described in the front.
3-hydroxyl-5-{[(1R is described below, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1:1):
3-hydroxyl-5-{[(1R, 2R)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazoles-3- Base) benzamide and 3-hydroxyl-5-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl -1H-pyrazole-3-yl) benzamide (1:1)
Figure A200810169286D01171
With 3-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide and 3-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (1:1) mixture (1.26g, 3.19mmol) and the solution of 10% weight palladium on carbon (0.13g) in ethanol (50mL) under hydrogen in stirring at room 16 hours.Solution is passed through
Figure A200810169286D0117183448QIETU
Filter and wash with methyl alcohol (100mL).With solution for vacuum concentration, obtained required compound (1.03g). 1H?NMR?δ(d 6-DMSO)：1.09(d，3H)，1.17(d，3H)，3.76(m，1H)，3.78(s，3H)，4.34(quin，1H)，6.48(t，1H)，6.56(d，1H)，6.93(t，1H)，7.05(t，1H)，7.60(d，1H)，9.66(s，1H)，10.67(s，1H)；m/z?306(M+H) +
3-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide and 3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (1:1)
Figure A200810169286D01181
With 3-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid and 3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] mixture (2.50g of phenylformic acid (1:1), 7.92mmol), 1-methyl isophthalic acid H-pyrazoles-3-amine (1.54g, 15.8mmol), HATU (3.92g, 10.3mmol) and DIPEA (2.76mL, 15.8mmol) solution in DMF (15mL) under atmospheric pressure stirred 16 hours in room temperature.Add entry (150mL), and solution is extracted with ethyl acetate (250mL).With ethyl acetate layer salt water washing, dry (MgSO 4), and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtain required product (1.26g) with the mixture wash-out of 50% ethyl acetate in hexane. 1H?NMR?δ(CDCl 3)：1.17(s，3H)，1.18(s，3H)，2.44(d，1H)，3.70(s，3H)，3.77(m，1H)，4.10(quin，1H)，4.99(s，2H)，6.64(t，1H)，6.75(d，1H)，6.96(t，1H)，7.03(t，1H)，7.22(d，1H)，7.31(m，5H)，8.68(s，1H)；m/z?396(M+H) +
3-{[(1R, 2R)-2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid and 3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (1:1)
Figure A200810169286D01182
To 3-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate and 3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] mixture (2.52g of methyl benzoate (1:1), 7.63mmol) in the solution in THF (40mL), add lithium hydroxide monohydrate (0.80g, 19.07mmol) solution in water (10mL).With mixture in stirring at room 16 hours.Remove THF in the vacuum, and gained solution is distributed between water (100mL) and ethyl acetate (250mL).Ethyl acetate layer is washed with salt solution (50mL), and dry (MgSO 4).Then water layer is adjusted to pH7 by adding 1M hydrochloric acid, and extracts with ethyl acetate (75mL).Ethyl acetate layer salt water washing, and dry (MgSO 4).Ethyl acetate layer is merged, and evaporation, required product (2.50g) obtained.
1H?NMR?δ(CDCl 3)：1.18(s，3H)，1.20(s，3H)，3.80(quin，1H)，4.14(quin，1H)，5.01(s，2H)，6.72(t，1H)，7.21(m，1H)，7.32(m，6H).
3-{[(1R, 2R)-2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate and 3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (1:1)
Figure A200810169286D01191
With 3-hydroxyl-5-{[phenyl methyl] the oxygen base } methyl benzoate (3.00g, 11.61mmol), (2R, 3S)-2,3-dimethyl ethylene oxide (3.04mL, 34.8mmol) and salt of wormwood (4.02g, 29.0mmol) solution in acetonitrile (60mL) in microwave reactor in 150 ℃ the heating 3 hours.Remove acetonitrile in the vacuum, and remaining oily matter is dissolved in the ethyl acetate (50mL), water (50mL), salt solution (50mL) washing, dry (MgSO 4) and flash to remaining oily matter.With resistates SiO 2Chromatography purification is used eluent ethyl acetate, has obtained required compound (2.52g). 1H?NMRδ(CDCl 3)：1.17(d，6H)，3.82(s，3H)，4.04(q，2H)，4.99(s，2H)，6.67(t，1H)，7.14(s，1H)，7.30(m，6H)；m/z330(M-H) -
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } description of methyl benzoate.
Embodiment 20:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide
Figure A200810169286D01192
With N-(1-ethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide (128mg, 0.4mmol) and 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (111mg, 0.48mmol) in acetonitrile (2mL) contain salt of wormwood (111mg, solution 0.8mmol) in microwave reactor in 160 ℃ the heating 6 hours.Reaction mixture is filtered, and filtrate evaporated under reduced pressure is extremely done, and by the silicon-dioxide chromatography purification, with the mixture wash-out of 50-100% ethyl acetate in hexane, has obtained required product, is white foam shape thing (176mg).
1H?NMR?δ(CDCl 3)：1.28(d，3H)，1.41(t，3H)，2.32(quin，2H)，3.35(s，3H)，3.49(m，2H)，4.02(q，2H)，4.17(t，2H)，4.30(t，2H)，4.56(q，1H)，6.76(d，1H)，6.88(t，1H)，7.18(s，1H)，7.28(d，1H)，7.32(t，1H)，8.09(d，1H)，8.18(d，1H)，8.65(s，1H)；m/z?514(M+H) +
N-(1-ethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl is described below] the oxygen base } preparation of benzamide:
N-(1-ethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide
Figure A200810169286D01201
At following 10% palladium on carbon (1.9g of argon gas, 50%wet) be added to N-(1-ethyl-1H-pyrazole-3-yl)-3-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] (19.1g is 46.7mmol) in the mixture in anhydrous THF (100mL) and ethanol (100mL) for benzamide.With the reaction mixture degassing, be placed under the hydrogen capsule and stirred 16 hours.Mixture is passed through diatomite filtration, and, obtain brown oil the filtrate evaporation.Resistates with the mixture wash-out of 40-65% ethyl acetate in hexane, obtains required product by the silica column chromatography purification, is limpid oily matter, its crystallization (11.35g) when placing.
1H?NMR?δ(CDCl 3)：1.21(d，6H)，1.38(t，3H)，3.32(s，3H)，3.39-3.51(m，3H)，3.98(q，2H)，4.44-4.51(m，1H)，6.54(s，1H)，6.72(d，1H)，6.92(s，2H)，7.26(d，1H)，8.18(s，1H)，8.85(s，1H)；m/z?320(M+H) +?318(M-H) -
N-(1-ethyl-1H-pyrazole-3-yl)-3-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] benzamide
HATU (23.5g 61.83mmol) is added to 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base phenylformic acid (16.28g, 51.53mmol) in, add DMF (140mL) then and be cooled to 0 ℃.Adding 1-ethyl-1H-pyrazoles-3-amine (6.86g, 61.8mmol), the adding DIPEA (21.3mL) that continues, and will be reflected under the argon gas in 0 ℃ of stirring 3 hours.The volume of solvent is reduced, and resistates is dissolved in the ethyl acetate (500mL), with citric acid (200mL), sodium hydrogen carbonate solution (150mL) and saturated brine solution (2 x 150mL) washing.Organic layer is separated dry (MgSO 4), filter and evaporation.By the silica column chromatography purification, with the mixture wash-out of 10-50% ethyl acetate in hexane, obtained this title compound, be light yellow oil (19.1g).
1H NMR δ (CDCl 3): 1.23 (d, 3H), 1.38 (t, 3H), 3.33 (s, 3H), 3.42 (dd, 1H), 3.50 (dd, 1H), 3.97 (q, 2H), 4.49 (sextet, 1H), 4.99 (s, 2H), 6.66 (t, 1H), 6.75 (d, 1H), 6.98 (s, 1H), 7.02 (s, 1H), 7.26 (d, 1H), 7.28-7.37 (m, 5H), 8.58 (s, 1H); M/z 410 (M+H) +
3-[(1S has been described in the front)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } benzoic preparation.
The preparation of 1-ethyl-1H-pyrazoles-3-amine is described in document [Chem.Heterocycl.Compd. (Engl.Transl.), 11,1975,212].
Embodiment 21:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide
Figure A200810169286D01211
With N-(1-ethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide (0.128g, 0.4mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (116mg, 0..48mmol), cesium carbonate (392mg, 1.2mmol) and bromine three (triphenylphosphine) copper (I) (38mg, 0.04mmol) mixture in DMA (5mL) stirred 4 hours in 160 ℃ in microwave reactor.Product is dissolved in ethyl acetate (20mL) and the water (15mL), and layer is separated.Organic layer is washed dry (MgSO with salt solution (50mL) 4), remove in filtration and the vacuum and desolvate, obtain brown oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture gradient elution of 70-100% ethyl acetate in hexane, obtain material requested, be limpid foam (76mg). 1H?NMR?δ(CDCl 3)：1.26(d，3H)，1.40(t，3H)，2.29(quin，2H)，3.34(s，3H)，3.42-3.53(m，2H)，4.01(q，2H)，4.18(t，2H)，4.54(q，1H)，4.64(t，2H)，6.74(t，2H)，7.04(s，1H)，7.22(s，1H)，7.27(d，1H)，7.30(d，1H)，8.04(d，1H)，8.25(d，1H)，8.47(s，1H)；m/z?480(M+H) +
N-(1-ethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl has been described in the front] the oxygen base } preparation of benzamide and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Embodiment 22:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-ethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide
Figure A200810169286D01221
HATU (304mg, 0.80mmol) be added to 5-[(3-{[(1-ethyl-1H-pyrazole-3-yl) amino] carbonyl-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenyl) the oxygen base] pyrazine-2-formic acid (0.4mmol) and azetidine hydrochloride (75mg, 0.89mmol) in the mixture in DMF (5mL), add DIPEA (0.278mL then, 1.6mmol), and will react and stir 16 hours.Reaction mixture is added in the ethyl acetate (20mL), with organic solution water (10mL), 2N citric acid (10mL), saturated sodium bicarbonate aqueous solution (10mL) and salt solution (10mL) washing, dry then (MgSO 4), filtering also, reduction vaporization becomes gum.Resistates by the silicon-dioxide chromatography purification, with the mixture wash-out of 70-100% ethyl acetate in hexane, is obtained required compound, be white foam shape thing (98mg). 1H?NMR?δ(CDCl 3)：1.27(d，3H)，1.40(t，3H)，2.31(quin，2H)，3.34(s，3H)，3.43-3.54(m，2H)，4.01(q，2H)，4.19(t，2H)，4.54(q，1H)，4.62(t，2H)，6.73(d，1H)，6.87(t，1H)，7.18(t，1H)，7.27(d，1H)，7.30(t，1H)，8.26(d，1H)，8.49(s，1H)，8.78(d，1H)；m/z?481(M+H) +
5-[(3-{[(1-ethyl-1H-pyrazole-3-yl is described below) amino] carbonyl }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenyl) the oxygen base] preparation of pyrazine-2-formic acid:
5-[(3-{[(1-ethyl-1H-pyrazole-3-yl) amino] carbonyl }-5-{[(1S)-1-methyl-2-(methoxyl group) Ethyl] the oxygen base } phenyl) the oxygen base] pyrazine-2-formic acid
Figure A200810169286D01231
With N-(1-ethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide (128mg, 0.4mmol) and methyl-5-chloro pyrazine-2-manthanoate (83mg, 0.48mmol) in acetonitrile (2mL) contain salt of wormwood (111mg, solution 0.8mmol) in microwave reactor in 160 ℃ the heating 6 hours.Reaction mixture is dissolved in the water (10mL), with the acidifying of 2N citric acid, with ethyl acetate (5 x 20mL) extraction.With organic phase salt water washing, dry (MgSO 4) and reduction vaporization to doing, obtain material requested, for yellow solid (164mg), it is used for next step under situation about not being further purified.m/z?442(M+H) +
N-(1-ethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl has been described in the front] the oxygen base } preparation of benzamide.
Embodiment 23:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-[1-(1-methylethyl)-1H-pyrazole-3-yl] benzamide
With 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-[1-(1-methylethyl)-1H-pyrazole-3-yl] benzamide (142mg, 0.23mmol) (0.2mL 0.68mmol) stirs 1 hour together for mixture in methyl alcohol (2mL) and 3.5M hydrochloric acid.Solution is neutralized with saturated sodium bicarbonate solution, and organic solvent is removed in decompression.Resistates extracts with ethyl acetate (3 x 10mL), dry (MgSO 4), filter and removal of solvent under reduced pressure.Resistates with the mixture wash-out of 75-100% ethyl acetate in hexane, has obtained required product by the silicon-dioxide chromatography purification, is white foam shape thing (99mg).
1H?NMR?δ(CDCl 3)：1.25(d，3H)，1.41(d，6H)，2.32(quin，2H)，3.67-3.71(m，2H)，4.17(t，2H)，4.26-4.35(m，3H)，4.52(q，1H)，6.73(s，1H)，6.87(s，1H)，7.19(s，1H)，7.30(s，1H)，7.32(s，1H)，8.10(s，1H)，8.18(s，1H)，8.56(s，1H)；m/z514(M+H) +
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl is described below] the oxygen base }-5-[((1S)-2-{[(1, and the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-[1-(1-methylethyl)-1H-pyrazole-3-yl] preparation of benzamide:
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-[1-(1-methylethyl)-1H-pyrazole-3-yl] benzamide
With 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-[1-(1-methylethyl)-1H-pyrazole-3-yl] benzamide (103mg, 0.24mmol) and 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (66mg, 0.29mmol) in acetonitrile (2mL), contain salt of wormwood (66mg, 0.48mmol) solution, in microwave reactor in 160 ℃ the heating 6 hours.Reaction mixture is filtered, and filtrate evaporated under reduced pressure is extremely done, and,, obtained required product, be white foam shape thing (142mg) with the mixture wash-out of 50-100% ethyl acetate in hexane by the silicon-dioxide chromatography purification.
m/z?628(M+H) +
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) The oxygen base]-5-hydroxy-n-[1-(1-methylethyl)-1H-pyrazole-3-yl] benzamide
Figure A200810169286D01242
With 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-[1-(1-methylethyl)-1H-pyrazole-3-yl]-the 5-[(phenyl methyl) the oxygen base] benzamide (1.97g, 3.77mmol) and the solution of THF (70mL) vacuumizes and purge with argon gas (x3).(10% w/w 400mg), vacuumizes reaction mixture and use at last hydrogen purge to add palladium on carbon.Reaction mixture was stirred 16 hours under hydrogen in room temperature.Cross elimination palladium on carbon and vacuum concentration, obtained product, be colorless oil (1.58g, 97%).
1H?NMR?δ(CDCl 3)：0.02(s，3H)，0.04(s，3H)，0.85(s，9H)，1.27(d，3H)，1.53(s，3H)，1.55(s，3H)，3.63(dd，1H)，3.77(dd，1H)，4.41(m，1H)，6.60(s，1H)，6.81(s，1H)，7.00(s，1H)，7.07(s，1H)，7.38(s，1H)，8.78(br.s，1H)；m/z?434(M+H) +，432(M-H) -.
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) The oxygen base]-N-[1-(1-methylethyl)-1H-pyrazole-3-yl]-the 5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D01251
DIPEA (3.11mL, 18.03mmol) be added to 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (3.00g, 7.21mmol), HATU (3.12g, 8.21mmol) and 1-(1-methylethyl)-1H-pyrazoles-3-amine (1.13g is 9.01mmol) in the solution in DMF (10mL).With the gained mixture in stirring at room 16 hours.Remove DMF in the vacuum.With solvent evaporation, and resistates is dissolved in 5%w/v citric acid (50mL) and ethyl acetate (30mL) and the ether (30mL), and organic layer is further used saturated sodium bicarbonate aqueous solution (30mL) and salt solution (30mL) washing.Organic layer is separated dry then (MgSO 4), filter and evaporation.By the column chromatography purifying, use the 1:4-1:3 ethyl acetate: the hexane wash-out, obtained this title compound, be colorless oil (2.40g, 65%). 1H?NMR?δ(CDCl 3)：0.01(s，3H)，0.03(s，3H)，0.86(s，9H)，1.24(d，3H)，1.49(s，3H)，1.51(s，3H)，3.64(dd，1H)，3.78(dd，1H)，4.39(m，1H)，4.46(m，1H)，5.09(s，2H)，6.70(s，1H)，6.78(s，1H)，7.02(s，1H)，7.08(s，1H)，7.35(m，6H)，8.32(br.s，1H)；m/z524(M+H) +，522(M-H) -.
1-(1-methylethyl)-1H-pyrazoles-3-amine
Figure A200810169286D01261
With the 2-chloroacrylonitrile (3.41mL, 42.59mmol) in room temperature be added to the N-sec.-propyl hydrazonium salt hydrochlorate that stirring (4.71g, 42.6mmol), (11.8g is 85.2mmol) in the solution in water (50mL) for salt of wormwood.With reaction be heated to 45 ℃ 4 hours, be cooled to RT then.Then organic layer is extracted with ethyl acetate (5 x 30mL), and with the organic layer drying (MgSO that merges 4), use activated carbon treatment, filter and evaporation.Resistates by chromatography purification, with the mixture wash-out of 67%-100% ethyl acetate in hexane, is obtained this title compound (3.08g, 58%), and the 6:1 mixture for true product and regional isomer is oily matter.This material is used under situation about not being further purified. 1H?NMR?δ(CDCl 3)：1.42(m，6H)，3.58(br.s，2H)，4.25(sept，1H)，5.58(d，1H)，7.15(d，1H).
3-[((1S has been described in the front)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] benzoic preparation.
Embodiment 24:3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide
Figure A200810169286D01262
With 1,1-dimethyl ethyl 3-{[(3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenyl) carbonyl] amino }-1H-pyrazoles-1-manthanoate (157mg, 0.4mmol) and 5-(azetidine-1-base carbonyl)-2-chloropyridine (95mg, 0.48mmol) in acetonitrile (2mL) contain salt of wormwood (111mg, solution 0.8mmol) in microwave reactor in 160 ℃ the heating 6 hours.Reaction mixture is filtered, and it is filtrate evaporated under reduced pressure is extremely dried, and by the silicon-dioxide chromatography purification, with the mixture wash-out of 50-100% ethyl acetate in hexane, obtain product, carry out purifying with the silicon-dioxide column chromatography again, make the mixture wash-out in ethyl acetate with 0-8% methyl alcohol, obtain required product, be white foam shape thing (21mg).
1H?NMR?δ(CDCl 3)：1.25(d，3H)，2.30(quin，2H)，3.33(s，3H)，3.42-3.54(m，2H)，4.12-4.29(m，4H)，4.54(q，1H)，6.77(s，1H)，6.81(t，1H)，6.97(d，1H)，7.20(s，1H)，7.27(s，1H)，7.33(d，1H)，8.01(d，2H)，8.28(d，1H)，10.27(s，1H)；m/z?452(M+H) +
Describe below 1,1-dimethyl ethyl 3-{[(3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenyl) carbonyl] amino }-preparation of 1H-pyrazoles-1-manthanoate:
11-dimethyl ethyl 3-{[(3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenyl) carbonyl] amino }-1H-pyrazoles-1-manthanoate
With 1,1-dimethyl ethyl 3-[({3-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenyl } carbonyl) amino]-(23g, 47.8mmol) solution in THF (140mL) and ethanol (140mL) vacuumizes and with nitrogen purging (x3) 1H-pyrazoles-1-manthanoate.Add 10% palladium on carbon (2.3g; 10%w/w), reaction mixture is vacuumized, and use hydrogen purge at last.Reaction mixture was stirred 16 hours under the hydrogen capsule in room temperature.Remove palladium on carbon by diatomite filtration, and, obtain white foam shape thing (18g, 97%) the filtrate vacuum concentration.
1H NMR δ (d 6-DMSO): 1.2 (d, 3H), 1.55 (s, 9H), 3.25 (s, 3H obscuredby water peak), 3.4-3.5 (m, 2H), 4.7 (m, 1H), 6.5 (s, 1H), 6.95 (d, 1H), 7.0 (s, 1H), 7.1 (s, 1H), 8.2 (d, 1H), 9.65 (s, 1H), 11.2 (s, br, 1H); M/z392 (M+H) +
1,1-dimethyl ethyl 3-[({3-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenyl } carbonyl) amino]-1H-pyrazoles-1-manthanoate
Figure A200810169286D01272
DIPEA (28.5mL, 164mmol) be added to 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } phenylformic acid (20.7g, 65.6mmol), HATU (31.2g, 82.0mmol) and 1,1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate (15.0g, 82.0mmol) in the suspension in DMF (30mL), and with reaction mixture in stirring at room 16 hours.Add entry (250mL), and reaction mixture is extracted with ether (3 x 150mL).Organic layer is washed with saturated brine solution, and dry (MgSO 4).With the filtrate vacuum concentration, and resistates crystallization when placing.Crystal is washed with isohexane, obtained material requested, be yellow crystals (23.4g; 73%).m/z?482(M+H) +.
1,1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate
Figure A200810169286D01281
(428mg 5.15mmol) is dissolved among the DMF (5mL), and (206mg 5.15mmol) handles, and then stirred 30 minutes with sodium hydride 1H-pyrazoles-3-amine in 0 ℃.(1.12g 5.15mmol), is heated to room temperature with reaction, and restir 2 hours with slowly adding the tert-Butyl dicarbonate that heats up in 5 minutes by syringe then.Reaction is dissolved in saturated sodium bicarbonate aqueous solution (50mL) and the ethyl acetate (100mL).Organic layer is separated dry then (MgSO 4), filter and evaporation.(use the 1:1 ethyl acetate: hexane-pure ethyl acetate wash-out), obtained this title compound (117mg), be white solid by the column chromatography purifying. 1H?NMR?δ(CDCl 3)：1.62(s，9H)，4.00(br.s，2H)，5.81(d，1H)，7.82(d，1H)
1,1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate, by with the solution of 1H-pyrazoles-3-amine in THF (1g approximately 15.6mL in) and the NaHMDS (solution of 2M in THF, 1.05 reaction equivalent), add the solution (approximately 1g two carbonic ethers solution among 1.9mLs) of tert-Butyl dicarbonate (1 equivalent) in THF then, and in stirring at room.With the crystallization from the mixture of ethyl acetate and isohexane of gained material.
3-[(1S has been described in the front)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } benzoic preparation.
Embodiment 25:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01282
With 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide (80mg, 0.26mmol), 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (61mg, 0.26mmol) and cesium carbonate (172mg, 0.53mmol) mixture in acetonitrile (3mL) stirred 3 hours in 160 ℃ in Smith Creator microwave reactor.Remove in the vacuum and desolvate, and ethyl acetate (50mL) is added in the resistates.With organic phase water (20mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-10% methyl alcohol in DCM, has been obtained this title compound, be yellow foam (52mg).
1H?NMR?δ(CDCl 3)：2.16-2.24(2H，m)，2.34-2.44(4H，m)，3.78(3H，s)，3.89-4.01(4H，m)，4.24(2H，t)，4.37(2H，t)，4.99(1H，s)，6.80(1H，s)，6.89(1H，s)，7.23(1H，s)，7.30(2H，d)，8.16(1H，s)，8.24(1H，s)，8.73(1H，s)；m/z?498(M+H) +.
5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine have been described in the front.
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base is described below] preparation of benzamide:
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01291
N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3-[(phenyl methyl) the oxygen base]-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide (453mg 1.15mmol) is dissolved in the ethanol (5mL), and disposable adding ammonium formiate (182mg, 2.88mmol).To react with argon gas and cover, and add 10% and drape over one's shoulders palladium activated carbon (30mg).This mixture heated 10 minutes in 140 ℃ in Smith Creator microwave reactor.Cross the elimination catalyzer, and remove volatile matter in the vacuum, obtained this title product, be white solid (339mg).
1H?NMR?δ(CDCl 3)：2.06-2.14(1H，m)，2.15-2.22(1H，m)，3.72-3.73(3H，s)，3.84-3.89(1H，m)，3.92-3.98(3H，m)，4.88(1H，m)，6.53(1H，t)，6.78(1H，d)，6.89(1H，s)，6.95(1H，s)，7.28(1H，d)，9.27(1H，s)；m/z?304(M+H) +.
N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3-[(phenyl methyl) oxygen base]-5-[(3S)-tetrahydrofuran (THF)-3-base The oxygen base] benzamide
Figure A200810169286D01301
With 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (450mg, 1.39mmol), (3R)-tetrahydrofuran (THF)-3-base 4-toluene sulfonic acide ester (507mg, 2.09mmol) and salt of wormwood (481mg, 3.48mmol) suspension in acetonitrile (5mL) stirred 3 hours in 160 ℃ in the SmithCreator microwave reactor.Remove in the vacuum and desolvate, and add ethyl acetate.With organism water (40mL), salt solution (40mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained yellow foam, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-100% ethyl acetate in isohexane, has been obtained this title compound, be white foam shape thing (452mg).
1H?NMR?δ(CDCl 3)：2.09-2.14(1H，m)，2.14-2.24(1H，m)，3.68(3H，s)，3.86-3.91(1H，m)，3.94-3.98(3H，m)，4.89(1H，s)，5.03(2H，s)，6.64(1H，t)，6.85(1H，s)，6.96(1H，d)，7.07(1H，t)，7.27(1H，m)，7.33-7.41(5H，m)，9.31(1H，s)；m/z?394(M+H) +.
(3R)-tetrahydrofuran (THF)-3-base 4-toluene sulfonic acide ester
Figure A200810169286D01302
4-tosyl group chlorine (1.65g, 8.63mmol) be added to the R-3-hydroxyl tetrahydrofuran (0.8g, 9.08mmol) and pyridine (0.88mL is 10.9mmol) in the solution in DCM (15mL).To react on stirring at room 72 hours.Add entry (10mL) and 1M hydrochloric acid (1mL), and mixture is extracted with DCM (15mL).Organic layer is washed dry (MgSO with salt solution (20mL) 4), filter and vacuum concentration, obtained yellow oil, with its silicon-dioxide chromatography purification,, obtained required compound (1.0g) with the mixture gradient elution of 0-50% ethyl acetate in isohexane. 1HNMR?δ(CDCl 3)：2.13(m，2H)，2.47(s，3H)，3.80-3.95(m，4H)，5.15(m，1H)，7.37(d，2H)，7.81(d，2H).
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) oxygen base] benzamide
Figure A200810169286D01311
N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3, and 5-two [(phenyl methyl) oxygen base] benzamide (1.0g 2.42mmol) is dissolved in the ethanol (12mL), and disposable adding ammonium formiate (229mg, 3.63mmol).Cover reaction with argon gas, and add 10% palladium carbon (10mg).With this mixture in Smith Creator microwave reactor, be heated to 140 ℃ 5 minutes.Cross the elimination catalyzer, and remove volatile matter in the vacuum, with resistates SiO 2Chromatography purification with the mixture gradient elution of 30-100% ethyl acetate in isohexane, has obtained this title compound, is white solid (378mg).
1H?NMR?δ(d 6-DMSO)：3.78(3H，s)，5.13(2H，s)，6.55-6.57(2H，m)，6.99(1H，s)，7.17(1H，s)，7.34-7.48(5H，m)，7.60(1H，d)，9.74(1H，s)，10.70(1H，s)；m/z?324(M+H) +.
N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3,5-two [(phenyl methyl) oxygen base] benzamide
Figure A200810169286D01312
(7.71mL 89.7mmol) is added drop-wise to 3, and (20.0g is 59.8mmol) in the suspension in DCM (0.5L) for 5-dibenzyl aminobenzoic acid at the following oxalyl chloride of argon gas.To react on stirring at room 6 hours, remove volatile matter then in a vacuum.Resistates is dissolved among the DCM (300mL), and drips 1-methyl isophthalic acid H-pyrazoles-3-amine (5.81g, 59.8mmol) solution in DCM (50mL).In stirring at room 16 hours, this time formed throw out later with gained solution.Solid filtering is separated, and crystallization from ethanol, obtained this title compound, be white solid (14.8g). 1H?NMR?δ(d 6-DMSO)：3.84(3H，s)，5.17(4H，s)，6.59(1H，d)，6.84(1H，t)，7.33-7.46(12H，m)，7.62(1H，d)，10.83(1H，s)；m/z?414(M+H) +.
Embodiment 26:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-methyl -1H-pyrazole-3-yl)-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide
With 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide (80mg, 0.26mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (64mg, 0.26mmol), bromine three (triphenylphosphine) copper (I) (49mg, 0.05mmol) and cesium carbonate (257mg, 0.78mmol) mixture in acetonitrile (3mL) stirred 6 hours in 160 ℃ in Smith Creator microwave reactor.Cross the elimination solid, and remove in the vacuum and desolvate, and ethyl acetate (50mL) is added in the resistates.With organic phase water (20mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtain yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture gradient elution of 0-10% methyl alcohol in DCM, obtained this title compound, be colorless solid (58mg).Adopt vapour phase hanging drop diffusion technique (vapour diffusion techniques) with the crystallization from ethyl acetate and isohexane of this compound. 1H?NMR?δ(CDCl 3)：2.11-2.17(1H，m)，2.20-2.29(1H，m)，2.32-2.39(2H，m)，3.78(3H，s)，3.88-3.93(1H，m)，3.96-4.02(3H，m)，4.25(2H，t)，4.71(2H，t)，4.97(1H，t)，6.73(1H，t)，6.79(1H，s)，7.11(1H，s)，7.22(1H，s)，7.27-7.30(1H，m)，7.36-7.39(1H，m)，8.12(1H，d)，8.32(1H，d)，8.73(1H，s)；m/z?464(M+H) +.
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base has been described in the front] preparation of benzamide and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Embodiment 27:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01322
With 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide (80mg, 0.26mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (52mg, 0.26mmol) and cesium carbonate (172mg, 0.53mmol) mixture in acetonitrile (3mL) stirred 1 hour in 160 ℃ in the SmithCreator microwave reactor.Remove in the vacuum and desolvate, and ethyl acetate (50mL) is added in the resistates.With organic phase water (20mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained yellow oil, with its silicon-dioxide chromatography purification,, obtained this title compound (36mg, 31%) with the mixture gradient elution of 0-10% methyl alcohol in DCM, be white foam shape thing.Adopt vapour phase hanging drop diffusion technique (vapourdiffusion techniques) with the crystallization from ethyl acetate and isohexane of this compound. 1H?NMR?δ(CDCl 3)：2.12-2.19(1H，m)，2.21-2.28(1H，m)，2.34-2.42(2H，m)，3.75(3H，s)，3.88-3.93(1H，m)，3.98-4.02(3H，m)，4.23(2H，t)，4.69(2H，t)，4.95-4.97(1H，m)，6.80(1H，d)，6.87(1H，t)，7.23(1H，d)，7.27-7.30(1H，m)，7.28-7.30(1H，m)，8.34(1H，d)，8.84(1H，d)，8.98(1H，s)；m/z?465(M+H) +.
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base has been described in the front] preparation of benzamide.
The preparation of 2-(azetidine-1-base carbonyl)-5-chloropyrazine is described below:
2-(azetidine-1-base carbonyl)-5-chloropyrazine
Figure A200810169286D01331
(1.55mL, (2.31g is 14.57mmol) in the mixture in DCM (40mL) for the 5-chloropyrazine-2-formic acid that DMF (2) is added to that 17.48mmol) continues oxalyl chloride.To react on stirring at room 2 hours, remove volatile matter in the vacuum then.Resistates is dissolved among the DCM (40mL), and add azetidine (1.08mL, 16.03mmol) and triethylamine (4.46mL, 32.06mmol).With mixture in stirring at room 72 hours.Remove volatile matter in the vacuum, and ethyl acetate (100mL) is added in the resistates.With organism water (100mL), citric acid (50mL), saturated sodium bicarbonate solution (50mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained yellow solid.With resistates SiO2 chromatography purification, with the mixture gradient elution of 50-100% ethyl acetate in isohexane, obtained required compound, be yellow solid (2.38g). 1H?NMR?δ(CDCl 3)：2.35-2.42(2H，m)，4.26(2H，t)，4.67(2H，t)，8.52(1H，d)，9.09(1H，d)；m/z?198(M+H) +.
5-chloropyrazine-2-formic acid
Figure A200810169286D01341
To methyl-5-chloro pyrazine-2-carboxylicesters (120mg, 0.70mmol) add in the solution in acetonitrile (2mL) and DMF (1mL) lithium chloride (295mg, 6.95mmol).With suspension in the SmithCreator microwave reactor, be heated to 160 ℃ 5 minutes, will react water (10mL) dilution then.Add saturated sodium bicarbonate solution (20mL), and with water layer ethyl acetate (30mL) extracting twice.Discard the organism of merging, and be adjusted to pH4 with 1N hydrochloric acid.With water ethyl acetate (20mL) extracting twice, and with the organism water (2 x 20mL), salt solution (10mL) washing and the dry (MgSO that merge 4).Remove volatile matter, obtained this title compound, be colorless solid (68mg).
1H?NMR?δ(CDCl 3)：7.20(1H，brs)，8.72(1H，s)，9.21-9.21(1H，m)；m/z?157(M-H) +.
Embodiment 28:3-{[4-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01342
Cesium carbonate (488mg, 1.5mmol) be added to 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (153mg, 0.5mmol) and 4-(azetidine-1-base carbonyl)-2-bromo-1,3-thiazole (206mg, 0.75mmol) in the solution in acetonitrile (5mL), and the mixture that will stir in Biotage Initiator Microwave instrument in 150 ℃ of heating 8 hours.Mixture is cooled to room temperature and normal pressure, pours in the water (75mL), and extract with ethyl acetate (3 x 25mL).The organic layer salt water washing that merges, dry (MgSO 4) and evaporation, with gained resistates chromatogram purification on silicon-dioxide, use eluent ethyl acetate, obtained material requested (95mg).
1H NMR δ (CDCl 3): 1.3 (d, 3H), 2.2 (m, 2H), 3.3 (s, 3H), 3.4-3.55 (m, 2H), 3.7 (s, 3H), 4.1 (t, 2H), 4.4 (t, 2H), 4.5 (m, 1H), 6.7 (s, 1H), 6.95 (s, 1H), 7.25 (s, 1H), 7.3 (s, 2H), 7.35 (s, 1H), 7.65 (s, 1H) and 8.8 (s, 1H); M/z472 (M+H) +.
Adopt similar method by 4-(azetidine-1-base carbonyl)-2-bromo-1,3-thiazoles and 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide makes following compound:
Figure A200810169286D01351
3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base has been described in the front]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
The preparation of 4-(azetidine-1-base carbonyl)-2-bromo-1,3-thiazoles is described below:
4-(azetidine-1-base carbonyl)-2-bromo-1,3-thiazoles
Figure A200810169286D01352
(752mg, (0.38mL 4.34mmol), adds DMF (1 one) then 3.62mmol) slowly to add oxalyl chloride in the solution in DCM (10mL) to 2-bromo thiazole-5-carboxylic acid.Mixture was stirred 4 hours, remove organism in the vacuum then, and with resistates and toluene (10mL) azeotropic.The crude product solvent in DCM (10mL), and slowly be added to the azetidine hydrochloride that stirring (404mg, 4.34mmol) and triethylamine (1.8mL is 13mmol) in the suspension in DCM (25mL).Mixture in stirring at room 2 hours, is removed organism then in a vacuum.Resistates is distributed between ethyl acetate (50mL) and water (25mL), organic layer is washed with salt solution (25mL), dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (500mg) with the mixture wash-out of 40% ethyl acetate in isohexane.
1H NMR δ (CDCl 3): 2.3 (m, 2H), 4.15 (m, 2H), 4.6 (m, 2H) and 8.0 (s, 1H); M/z 249 (M+H) +.
Embodiment 29:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-(hydroxymethyl) propyl group] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01361
With 5-[(3-{[(1S)-1-(hydroxymethyl) propyl group] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid (12mg, 0.03mmol), azetidine hydrochloride (4mg, 0.06mmol), HATU (14mg, 0.04mmol) and DIPEA (0.02mL, 0.11mmol) solution in DMF (1mL) is spent weekend in stirring at room.Add (15mL) water, and solution is extracted with ethyl acetate (25mL).Ethyl acetate layer is washed dry (MgSO with salt solution (20mL) 4), and evaporation becomes resistates, by the anti-phase preparation of C18 HPLC chromatogram purification, the mixture wash-out of usefulness 5-95% acetonitrile (+0.2% TFA) in water (+0.2% TFA) obtained required product with it, and the impurity (5mg) that gets off of 15% co-elute. 1H NMR δ (CDCl 3): 0.92 (t, 3H), 0.97 (t, 0.45H), 1.69 (quin, 2H), 1.76 (m, 0.3H), 2.34 (quin, 2H), 3.76 (m, 2H), 3.86 (s, 3H), 4.23 (t, 2H), 4.49 (sextet, 1H), 4.66 (t, 2H), 4.77 (m, 0.15H), 6.93 (t, 1H), 6.99 (d, 1H), 7.34 (s, 1H), 7.46 (s, 1H), 7.50 (s, 1H), 8.29 (s, 1H), 8.78 (s, 1H), 10.68 (s, 1H), 10.78 (s, 0.15H); M/z 468 (M+H) +
Spectroscopic data and 15% following impurity 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(2S)-and the 2-hydroxybutyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide is consistent.
5-[(3-{[(1S is described below)-1-(hydroxymethyl) propyl group] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation in pyrazine-2-formic acid:
5-[(3-{[(1S)-and 1-(hydroxymethyl) propyl group] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) ammonia Base] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid
Lithium hydroxide monohydrate (6mg, 0.14mmol) mixture in water (1mL) is added to 5-[(3-{[(1S)-1-(hydroxymethyl) propyl group] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] (25mg is 0.06mmol) in the solution in THF (1mL) for pyrazine-2-methyl-formiate.With mixture in stirring at room 16 hours.Remove THF in the vacuum, and gained solution is distributed between water (10mL) and ethyl acetate (25mL), ethyl acetate layer is washed with salt solution (10mL) and dry (MgSO 4).By adding 1M hydrochloric acid water layer is adjusted to pH7, and extracts with ethyl acetate (25mL).Ethyl acetate layer is washed with salt solution (10mL) and dry (MgSO 4).Acetic acid ethyl acetate extract is merged, and evaporation, required product (12mg) obtained.m/z?428.44(M+H) +
Methyl 5-[(3-{[(1S)-and 1-(hydroxymethyl) propyl group] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-carboxylicesters
Figure A200810169286D01371
5-chloropyrazine-2-methyl-formiate (104mg, 0.60mmol) and cesium carbonate (467mg, 1.43mmol) be added to 3-hydroxyl-5-{[(1S)-1-(hydroxymethyl) propyl group] the oxygen base-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (175mg, 0.570mmol) in the solution in acetonitrile (5mL), and with mixture in microwave reactor in 160 ℃ of heating 330 minutes.Remove acetonitrile in the vacuum, and resistates is dissolved in the ethyl acetate (25mL), water (20mL), salt solution (20mL) washing, dry (MgSO 4), and evaporation becomes resistates, by the anti-phase preparation of C18 HPLC chromatography purification, the mixture wash-out of usefulness 5-95% acetonitrile (+0.2% TFA) in water (+0.2% TFA) obtained required product (41mg) with it. 1H?NMR?δ(CDCl 3)：0.97(t，3H)，1.78(quin，2H)，3.82(s，3H)，3.91(s，3H)，4.52(m，3H)，4.66(quin，1H)，6.59(t，1H)，6.89(d，1H)，7.05(t，1H)，7.15(t，1H)，8.19(d，1H)，8.79(d，1H)，9.86(s，1H)；m/z442(M+H) +
The 3-hydroxyl-5-{[(1S)-1-(hydroxymethyl) propyl group] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01372
To 3-hydroxyl-5-({ (1S)-1-[(methoxyl group) methyl] propyl group } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (500mg, 1.6mmol) in the solution in acetonitrile (25mL), add iodine trimethyl silane (1.11mL, 7.8mmol), and with gained mixture stirring 16 hours.Add saturated sodium bicarbonate solution (10mL),, add saturated aqueous sodium thiosulfate (5mL), remove acetonitrile in the vacuum then solution stirring 10 minutes.Remaining water layer is extracted with ethyl acetate (3 x 40mL), and water layer is merged, dry (MgSO 4), filter, evaporation, and,, obtained this title compound with the mixture wash-out of 85% ethyl acetate in isohexane by the column chromatography purifying, be colourless foam shape thing (405mg). 1H?NMR?δ(d 6-DMSO)：0.95(t，3H)，1.5-1.8(m，2H)，3.5(m，2H)，3.8(s，3H)，4.3(m，1H)，4.8(t，1H)，6.45(s，1H)，6.55(s，1H)，6.9(s，1H)，7.05(s，1H)，7.55(s，1H)，9.6(s，1H)；m/z?306(M+H) +
3-hydroxyl-5-((1S)-and the 1-[(methoxyl group) methyl] propyl group } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01381
To 3-({ (1S)-1-[(methoxyl group) methyl] propyl group } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (4.6g, 11mmol) at 1:1 THF: in the solution in the methyl alcohol (100mL), add 10% w/w palladium on carbon (450mg), and the gained mixture was stirred 6 hours under hydrogen.Substitute hydrogen with argon gas, mixture is filtered, evaporates, obtained this title compound, be white solid (3.6g).
1H?NMR?δ(CDCl 3)：0.95(t，3H)，1.7(m，2H)，3.4(s，3H)，3.55(m，2H)，3.8(s，3H)，4.3(m，1H)，6.65(s，1H)，6.8(s，1H)，7.0(m，2H)，7.2(m，1H)，7.3(s，1H)，8.7(s，1H)；m/z?320(M+H) +
3-((1S)-and the 1-[(methoxyl group) methyl] propyl group } the oxygen base)-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D01382
To 3-({ (1S)-1-[(methoxyl group) methyl] propyl group } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (4.75g, 14.4mmol) and 3-amino-1-methyl isophthalic acid H-pyrazoles (2.04g, 21mmol) add HATU (8.53g in the solution in DMF (25mL), 22.4mmol), add DIPEA (7.0mL then, 40mmol), and with the gained mixture stirred 16 hours.And mixture distributed between ethyl acetate (100mL) and water (30mL).Organic layer is separated, with 1N citric acid (30mL), water (30mL), saturated sodium bicarbonate (30mL), water (30mL) and salt solution (30mL) washing, dry then (MgSO 4) and evaporation.The gained resistates with the mixture wash-out of 50% ethyl acetate in isohexane, has obtained this title compound by the column chromatography purifying, is colorless oil (4.57g).
1H?NMR?δ(CDCl 3)：0.95(t，3H)，1.7(m，2H)，3.4(s，3H)，3.55(m，2H)，3.8(s，3H)，4.3(m，1H)，5.05(s，2H)，6.75(s，1H)，6.8(s，1H)，7.05(d，2H)，7.25(s，1H)，7.4(m，5H)，8.45(s，1H)；m/z?410(M+H) +
3-((1S)-and the 1-[(methoxyl group) methyl] propyl group } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] phenylformic acid
Figure A200810169286D01391
To 3-({ (1S)-1-[(methoxyl group) methyl] propyl group } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (6.85g, 20mmol) at 3:1 THF: in the solution of methyl alcohol (100mL), add the solution (40mL of 1N lithium hydroxide solution in water, 40mmol), and then intermittently add 100mL water in batches, simultaneously the gained mixture was stirred 2 hours.Remove organic solvent by evaporation, and turbid solution is filtered.By adding the pH regulator to 3 of 2M hydrochloric acid with solution.(3 * 70mL) extract with ethyl acetate with it.With the organic extract liquid drying (MgSO that merges 4) and evaporation, obtained this title compound, be the colorless oil (6.36g) of solidifying.
1H?NMR?δ(CDCl 3)：0.95(t，3H)，1.7(m，2H)，3.4(s，3H)，3.55(m，2H)，4.3(m，1H)，5.05(s，2H)，6.8(s，1H)，7.3-7.5(m，7H)；m/z?329(M-H) -
3-((1S)-and the 1-[(methoxyl group) methyl] propyl group } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D01401
With the 3-hydroxyl-5-{[phenyl methyl that is stirring] the oxygen base } methyl benzoate (7.5g, 29mmol), (R)-1-methoxyl group-Ding-2-alcohol (3.76g, 36.25mmol) and triphenylphosphine (9.5g, 36.25mmol) solution in anhydrous THF (75mL) cools off in ice bath, and dripped 40%DEAD at toluene (15.8mL, 36.25mmol) solution in 30 minutes.Reaction mixture slowly is heated to 10 ℃, and stirred 16 hours.With the THF evaporation, resistates is dissolved in the mixture of 30% ethyl acetate in isohexane, and in ice bath, cools off.By removing by filter the gained throw out, and with the mixture washing of 10% ethyl acetate in isohexane.With the filtrate evaporation, and,, obtained this title compound, be colorless oil (6.85g) with the mixture wash-out of 10% ethyl acetate in isohexane by the column chromatography purifying.
1H?NMR?δ(CDCl 3)：0.95(t，3H)，1.7(m，2H)，3.35(s，3H)，3.55(m，2H)，3.9(s，3H)，4.3(m，1H)，5.05(s，2H)，6.8(s，1H)，7.25(m，2H)，7.4(m，5H)；m/z?345(M+H) +
[Coke, J.L.; Shue, R.S., J.Org.Chem.38, (1973), 2210-2211] in the preparation of (R)-1-methoxyl group-Ding-2-alcohol has been described.
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate.
Embodiment 30:3-{[2-(azetidine-1-base carbonyl) pyrimidine-5-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D01402
With 3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base }-N-1,3-thiazol-2-yl benzamide (130mg, 0.42mmol), 2-(azetidine-1-base carbonyl)-5-bromo pyrimi piperidine (113mg, 0.46mmol), cesium carbonate (412mg, 1.26mmol) and bromine three (triphenylphosphine) copper (I) (118mg 0.13mmol) stirred 4 hours in 160 ℃ in microwave reactor at the mixture of DMA (5mL).
(50mL) is added in the resistates ethyl acetate, and with organism water (20mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained brown oil.With resistates SiO 2Chromatography purification with the mixture gradient elution of 0-10% methyl alcohol in ethyl acetate, has obtained required compound (67mg). 1H NMR δ (CDCl 3): 1.34 (d, 3H), 2.38 (quintet, 2H), 3.40 (s, 3H), 3.49-3.61 (m, 2H), 4.29 (t, 2H), 4.59-4.67 (m, 3H), 6.92 (t, 1H), 6.98 (d, 1H), 7.23 (d, 1H), 7.44 (t, 1H), 7.49 (t, 1H), 8.54 (s, 2H), 11.96 (s, 1H); M/z 470 (M+H) +
3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base has been described in the front }-N-1, the preparation of 3-thiazol-2-yl benzamide.
The preparation of 2-(azetidine-1-base carbonyl)-5-bromo pyrimi piperidine is described below:
2-(azetidine-1-base carbonyl)-5-bromo pyrimi piperidine
Figure A200810169286D01411
Successively oxalyl chloride (1.50mL, 16.79mmol), DMF (2) is added to 5-bromo pyrimi piperidine-2-carboxylic acid (2.86g is 14.0mmol) in the mixture in DCM (40mL).To react on stirring at room 2 hours, remove volatile matter in the vacuum, and resistates will be dissolved among the DCM (40mL).Successively add the azetidine hydrochloride (1.44g, 15.39mmol), triethylamine (4.29mL, 30.78mmol), and with mixture in stirring at room 72 hours.With the mixture vacuum concentration, and ethyl acetate (100mL) is added in the resistates.With organism water (100mL), citric acid (50mL), saturated sodium bicarbonate solution (50mL), salt solution (50mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained yellow solid.With this solid silicon-dioxide chromatography purification, with the mixture gradient elution of 50-100% ethyl acetate in isohexane, obtained required compound, be yellow solid (0.86g).
The preparation of 5-bromo pyrimi piperidine-2-carboxylic acid has been described in the document (International Patent Application WO 2005/028452).
Embodiment 31:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-(cyclopentyloxy)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
3-(cyclopentyloxy)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (200mg, 0.67mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (159mg, 0.80mmol) and salt of wormwood (184mg 1.33mmol) dissolves/is suspended in the acetonitrile (3.5mL).Reaction mixture was heated 4 hours in 120 ℃ in microwave reactor.With the reaction mixture cooling, filter and vacuum concentration.With crude product silicon-dioxide chromatography purification, with the mixture wash-out of 0-5% methyl alcohol in DCM, obtained required product, be white foam shape thing (282mg).
1H?NMR?δ(d 6-DMSO)：1.61(m，2H)，1.74(m，4H)，1.94(m，2H)，2.30(m，2H)，3.78(s，3H)，4.10(t，2H)，4.57(t，2H)，4.94(m，1H)，6.57(d，1H)，7.01(t，1H)，7.42(m，1H)，7.47(m，1H)，7.60(d，1H)，8.55(d，1H)，8.68(d，1H)，10.80(s，1H). m/z?463(M+H) +
Adopt similar method by 3-(cyclopentyloxy)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 5-(azetidine-1-base carbonyl)-2, the 3-dichloropyridine makes following compound.
Figure A200810169286D01422
2-(azetidine-1-base carbonyl)-5-chloropyrazine and 5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine have been described in the front.
The preparation of 3-(cyclopentyloxy)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide is described below:
3-(cyclopentyloxy)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01423
3-(cyclopentyloxy)-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] (1.87g 4.78mmol) is dissolved in the ethanol (40mL) benzamide, and adds 10% palladium on carbon (102mg) catalyzer under argon gas.Gained is reflected under the hydrogen stirred 86 hours, pass through then Filter, and vacuum concentration is light brown solid (1.31g). 1H?NMR?δ(d 6-DMSO)：1.54(m，2H)，1.76(m，4H)，1.96(m，2H)，2.75(s，1H)，3.83(s，3H)，4.91(m，1H)，6.49(m，1H)，6.61(m，1H)，6.98(m，1H)，7.06(m，1H)，7.65(s，1H)，9.73(brs，1H)；m/z?302(M+H) +
3-(cyclopentyloxy)-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) oxygen base] benzamide
Figure A200810169286D01432
3-(cyclopentyloxy)-5-[(phenyl methyl) the oxygen base] phenylformic acid (3.14g, 10.0mmol), 1-methyl isophthalic acid H-pyrazoles-3-amine (1.95g, 20mmol) and HATU (4.95g 13mmol) is dissolved in DMF (12.5mL), add then DIPEA (3.49mL, 20mmol).With the gained mixture in stirring at room 20 hours.Mixture water (150mL) is ended, and, used the salt water washing, dry (MgSO with ethyl acetate (2 x 75mL) extraction 4), filter and vacuum concentration, obtained yellow oil.With resistates SiO 2Chromatography purification with the mixture wash-out of 0-30% ethyl acetate in isohexane, obtains required product, is limpid gum (1.87g).
1H?NMR?δ(CDCl 3)：1.59(m，4H)，1.83(m，4H)，3.79(s，3H)，4.76(m，1H)，5.08(s，2H)，6.66(t，1H)，6.82(m，1H)，7.01(m，1H)，7.08(m，1H)，7.26(m，1H)，7.33(m，1H)，7.35-7.45(m，4H)，8.67(s，1H)；m/z392(M+H) +
3-(cyclopentyloxy)-5-[(phenyl methyl) oxygen base] phenylformic acid
Figure A200810169286D01433
3-(cyclopentyloxy)-5-[(phenyl methyl) the oxygen base] (9.25g 28.34mmol) is dissolved among the THF (120mL) methyl benzoate, and adds lithium hydroxide monohydrate (3.49g, 85.0mmol) solution in water (60mL).This biphasic solution in stirring at room 16 hours (LCMS show that reaction finish 80%), is added methyl alcohol (15mL), and with mixture restir 4 hours.Remove THF in the vacuum, add entry (40mL) then, and with hydrochloric acid with pH regulator to 7.Collect solid, and thoroughly use cold water (8.85g) washing.
1H?NMR?δ(d 6-DMSO)：1.64(m，2H)，1.76(m，4H)，1.96(m，2H)，4.89(m，1H)，5.19(s，2H)，6.80(s，1H)，7.07(s，1H)，7.16(s，1H)，7.34-7.53(m，5H)；m/z?311(M+H) +
3-(cyclopentyloxy)-5-[(phenyl methyl) oxygen base] methyl benzoate
Figure A200810169286D01441
With 3-hydroxyl-5-{[phenyl methyl] the oxygen base } methyl benzoate (10g, 38.7mmol), 1-cyclopentanol (6.135mL, 58.07mmol) and triphenylphosphine (15.24g, 58.07mmol) stirring in THF (166mL) under argon gas, and in ice bath, be cooled to 5 ℃.(25.3mL 58.1mmol) is added drop-wise in the mixture, internal temperature is remained on 5-10 ℃ simultaneously DEAD.Stirring is proceeded 16 hours.With the mixture vacuum concentration, be dissolved in again in ethyl acetate (60mL) and the isohexane (60mL), remove the gained throw out, and, obtained yellow oil solution for vacuum concentration.With resistates SiO 2Chromatography purification with the mixture wash-out of 0-30% ethyl acetate in isohexane, has obtained colorless oil, and its crystallization under vacuum becomes white solid.
1H?NMR?δ(CDCl 3)：1.62(m，2H)，1.71-1.98(m，6H)，3.90(s，3H)，4.76(m，1H)，5.08(s，2H)，6.69(m，1H)，7.16(m，1H)，7.23(m，1H)，7.29-7.44(m，5H)；m/z?325(M+H) +
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate.
Embodiment 32:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-(cyclopentyloxy)-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01451
With 3-(cyclopentyloxy)-5-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (200mg, 0.67mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (193mg, 0.80mmol), bromine three (triphenylphosphine) copper (186mg, 0.2mmol) and cesium carbonate (230mg 0.70mmol) dissolves/is suspended among the DMA (3.5mL).Reaction mixture was heated cooling then, filtration and vacuum concentration 40 minutes in 200 ℃ in microwave reactor.Crude product by the silicon-dioxide chromatography purification, with the mixture wash-out of 0-100% ethyl acetate in isohexane, has been obtained required compound, be light yellow foam (176mg). 1H?NMR?δ(d 6-DMSO)：1.61(m，2H)，1.73(m，4H)，1.94(m，2H)，2.28(m，2H)，3.78(s，3H)，4.08(t，2H)，4.59(t，2H)，4.95(m，1H)，6.56(d，1H)，6.88(t，1H)，7.28(m，1H)，7.43(t，1H)，7.56(m，1H)，7.59(d，1H)，7.99(d，1H)，8.41(m，1H)，10.85(s，1H)；m/z?462(M+H) +
Embodiment 33:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1:1)
Figure A200810169286D01452
DIPEA (0.26mL, 1.5mmol) be added to 5-[(3-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid and 5-[(3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid (1:1) (0.16g, 0.37mmol), azetidine hydrochloride (71mg, 0.75mmol) and HATU (299mg, 0.79mmol) in the mixture in DMF (5mL), and in stirring at room 72 hours.Add ethyl acetate (40mL), and with mixture water (2 x 30mL), salt solution (30mL) washing, dry (MgSO 4), filter and vacuum concentration, obtained yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture washing of 0-100% ethyl acetate in isohexane, has been obtained this title compound of diastereomer, be colorless oil.Mixture is prepared HPLC with chirality separate on Chiralpak IA (250mmx20mm) EG014 post, with the mixture wash-out in the 30% ethanol t-butyl methyl ether.The diastereomer (embodiment 33a) of first wash-out is separated, be white foam shape thing (18mg).
1H?NMR?δ(CDCl 3)：1.19(d，3H)，1.23(d，3H)，2.26-2.34(m，3H)，3.74(s，3H)，3.77-3.85(m，1H)，4.13-4.23(m，3H)，4.63(t，2H)，6.72(d，1H)，6.86(t，1H)，7.16-7.22(m，2H)，7.27-7.30(m，1H)，8.27(d，1H)，8.31(s，1H)，8.79(d，1H)；m/z?467(M+H) +
The diastereomer (embodiment 33b) of second wash-out is separated, be white foam shape thing (19mg).
1H?NMR?δ(CDCl 3)：1.19(d，3H)，1.22(d，3H)，2.27-2.36(m，3H)，3.73(s，3H)，3.76-3.84(m，1H)，4.13-4.22(m，3H)，4.62(t，2H)，6.72(d，1H)，6.86(t，1H)，7.17-7.19(m，1H)，7.21(d，1H)，7.28(t，1H)，8.26(d，1H)，8.40(s，1H)，8.79(d，1H)；m/z?467(M+H) +
5-[(3-{[(1R is described below, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid and 5-[(3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] preparation of mixture of pyrazine-2-formic acid (1:1):
5-[(3-{[(1R, 2R)-2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) Amino] carbonyl phenyl) the oxygen base] pyrazine-2-formic acid and
5-[(3-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) Amino] carbonyl } phenyl) the oxygen base] pyrazine-2-formic acid (1:1)
Figure A200810169286D01461
Lithium hydroxide monohydrate (38mg, 0.88mmol) solution in water (3mL) is added to 5-[(3-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-methyl-formiate and 5-[(3-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-methyl-formiate (1:1) (0.26g, 0.59mmol) in the mixture in THF (6mL), and with mixture in stirring at room 72 hours.Remove THF in the vacuum, and aqueous residue is adjusted to pH3 with citric acid, be extracted into then in the ethyl acetate, water (30mL), salt solution (30mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained required compound, be white solid (0.16g).
1H?NMR?δ(d 6-DMSO)：1.16(d，3H)，1.26(d，3H)，3.84(s，3H)，4.20(m，1H)，4.49(m，1H)，4.89(d，1H)，6.64(d，1H)，7.13(t，1H)，7.49(s，1H)，7.59(s，1H)，7.67(d，1H)，8.72(d，1H)，8.85(d，1H)，10.93(s，1H)，13.53(s，1H)；m/z?426(M-H) -
Methyl 5-[(3-{[(1R, 2R)-2-hydroxyl-1-methyl-propyl] the oxygen base-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl phenyl) the oxygen base] pyrazine-2-carboxylicesters and
Methyl 5-[(3-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-5-{[(1-methyl isophthalic acid H-pyrazole-3-yl) amino] carbonyl } phenyl) the oxygen base] pyrazine-2-carboxylicesters (1:1)
Figure A200810169286D01471
With 3-hydroxyl-5-{[(1R, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-{[(1S, 2S)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1:1) (230mg, 0.75mmol), methyl 5-chloropyrazine-2-carboxylicesters (195mg, 1.13mmol) and salt of wormwood (208mg, 1.51mmol) mixture in acetonitrile (5mL) stirred 3 hours in 130 ℃ in microwave reactor.Remove in the vacuum and desolvate, and add entry.With the mixture acidifying, be extracted in the ethyl acetate, and with the organism salt water washing that merges, dry (MgSO 4), filter and vacuum concentration, obtained yellow foam (265mg), it is used for next step under situation about not being further purified. 1H NMR δ (CDCl 3): 1.29 (m, 6H), 1.99 (s, 1H), 3.80 (s, 3H), 3.90 (quintet, 1H), 4.05 (s, 3H), 4.25 (quintet, 1H), 6.82 (d, 1H), 6.95 (t, 1H), 7.30 (m, 2H), 7.39 (s, 1H), 8.55 (s, 1H), 8.55 (s, 1H), 8.86 (s, 1H); M/z 442 (M+H) +
3-hydroxyl-5-{[(1R has been described in the front, 2R)-and 2-hydroxyl-1-methyl-propyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and 3-hydroxyl-5-{[(1S, 2S)-2-hydroxyl-1-methyl-propyl] the oxygen base }-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (1:1).
Embodiment 34:3-{[5-(azetidine-1-base carbonyl)-1,3-thiazoles-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01481
Cesium carbonate (489mg, 1.5mmol) be added to 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base-N-(5-methylpyrazine-2-yl) benzamide (159mg, 0.5mmol) and 5-(azetidine-1-base carbonyl)-2-chloro-1,3-thiazole (152mg, 0.75mmol) in the solution in acetonitrile (5mL), and the mixture that will stir in microwave reactor in 120 ℃ of heating 1 hour.Mixture is cooled to room temperature and normal pressure, removes acetonitrile in the vacuum, resistates distributes between water (25mL) and ethyl acetate (50mL), organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide, use eluent ethyl acetate, obtain required product (181mg).
1H NMR δ (CDCl 3): 1.3 (d, 3H), 2.35 (m, 2H), 2.5 (s, 3H), 3.3 (s, 3H), 3.45-3.55 (m, 2H), 4.1-4.4 (m, 4H), 4.55 (m, 1H), 7.0 (d, 1H), 7.35 (d, 1H), 7.45 (s, 1H), 7.45 (s, 1H), 8.05 (s, 1H), 8.4 (s, 1H) and 9.45 (s, 1H) .m/z 484 (M+H) +.
Adopt similar method by 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide or 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide and suitable chloro-substituted heterocyclic ring make following compound:
Figure A200810169286D01491
*Compound is partial crystallization from ether; Mpt: 80.7 ℃ of fusing beginnings.
* *Embodiment 34b, 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide, also be to adopt following method preparation.
With 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide (25.4g, 80.0mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (17.4g, 88.0mmol) and salt of wormwood (33.1g, 240mmol) mixture in acetonitrile (254mL) in 60 ℃ the heating 24 hours.Desolvate by evaporating to remove, and resistates is distributed between ethyl acetate (15vol) and water (10vol).Layer is separated, water layer is extracted in the ethyl acetate (2 x 10vol) again, and with the organism water (2 x 5vol), salt solution (5vol) washing that merge, dry (MgSO 4), filter, concentrate in the vacuum.With resistates SiO 2Chromatography purification is used eluent ethyl acetate, has obtained required compound, is foam (33.7g).This pure material is dissolved in the t-butyl methyl ether (30vols) of heat, add real crystalline sample (from the small-scale crystallization, to isolate crystal seed as crystal seed, wherein be that product is dissolved in the t-butyl methyl ether of heat of minimum aequum, add isohexane then, the scraping flask is to promote crystallization) and stir and spend the night.Collect crystallized product (110.3 ℃ of fusing beginnings) (29.1g) with the mixture cooling and by filtering.
1H?NMR?δ(d 6-DMSO)：1.27(d，3H)，2.30(quin，2H)，2.49(s，3H)，3.31(s，3H)，3.47-3.56(m，2H)，4.10(t，2H)，4.57(t，2H)，4.79(td，1H)，7.13(t，1H)，7.48(td，1H)，7.57(td，1H)，8.36(d，1H)，8.56(d，1H)，8.69(d，1H)，9.26(d，1H)，11.00(s，1H)；m/z?479(M+H) +
Can by the following method this crystalline form be changed into different crystal forms: about 30mg material is placed have the magnetic flea bottle of (magnetic flea), and add about 1mL water.Drip methyl alcohol to wherein adding several (3), the bottle plug seal.Allow these slurries on magnetic sheet, stir then in room temperature (25 ℃).After 3 days, sample is removed from magnetic sheet, taken off stopper, it is dry to allow slurries be under the ambient environmental conditions.Understand that by the XRPD analytical table new crystalline form (beginning fusing at 110.2 ℃) is a different crystal forms.
Also can make this title compound form crystallization by the slurries of compound in water are stirred.
3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl is described below] the oxygen base }-preparation of N-(5-methylpyrazine-2-yl) benzamide:
The 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01501
(700mg) is added to 3-{[(1S 10% palladium on carbon)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(phenyl methyl) the oxygen base] benzamide (7.0g, 17.2mmol) in the solution in ethanol (125mL), and mixture stirred 4 hours under hydrogen in room temperature.Remove by filter catalyzer, and ethanol is evaporated in a vacuum.With resistates crystallization from ethyl acetate, obtained required compound (4.22g). 1H NMR δ (CDCl 3): 1.25 (d, 3H), 2.5 (s, 3H), 3.3 (s, 3H), 3.4-3.5 (m, 2H), 4.5 (m, 1H), 6.3 (br, 1H), 6.55 (s, 1H), 6.9 (s, 1H), 6.95 (s, 1H), 8.05 (s, 1H), 8.45 (s, 1H) and 9.5 (s, 1H) .m/z 318 (M+H) +.
Also can be with methyl alcohol as solvent and use 5bar pressure hydrogen to carry out this reaction.
3-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D01511
Oxalyl chloride (2.1mL 24.0mmol) is added to 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base phenylformic acid (6.32g, 20.0mmol) in the solution in DCM (100mL), and with mixture in stirring at room 4 hours.Compound is evaporated to resistates in a vacuum, it is dissolved among the DCM (25mL), and in 5 ℃-10 ℃ be added to 2-amino-5-methylpyrazine of stirring (2.29g, 21.0mmol) and pyridine (1.94mL is 24.0mmol) in the mixture in DCM (100mL).Mixture in stirring at room 18 hours, and is evaporated DCM in a vacuum.Resistates is distributed between water (50mL) and ethyl acetate (150mL), and with organic layer salt water washing, dry (MgSO 4) and be evaporated to resistates, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 50% ethyl acetate in isohexane, has been obtained required compound (7.0g). 1H NMR δ (CDCl 3): 1.3 (d, 3H), 2.5 (s, 3H), 3.3 (s, 3H), 3.4-3.5 (m, 2H), 4.5 (m, 1H), 5.0 (s, 2H), 6.7 (s, 1H), 7.0 (s, 1H), 7.05 (s, 1H), 7.35 (m, 5H), 8.05 (s, 1H), 8.3 (s, 1H) and 9.5 (s, 1H) .m/z 408 (M+H) +.
The preparation of 2-amino-5 methylpyrazine has been described in the document [Tetrahedron Lett.2002,9287].
3-[(1S has been described in the front)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } benzoic preparation.
3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base has been described in the front]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
Halogenated heterocyclic 5-(azetidine-1-base carbonyl)-2-chloro-1 has been described in the front, the 3-thiazole, 5-(azetidine-1-base carbonyl)-2, the 3-dichloropyridine, 2-(azetidine-1-base carbonyl)-5-chloropyrazine, the preparation of 5-(azetidine-1-base carbonyl)-2-chloropyridine and 4-(azetidine-1-base carbonyl)-2-bromo-1,3-thiazoles.
5-(azetidine-1-base the carbonyl)-2-bromo-4-methyl isophthalic acid that is used for embodiment 34e-f is described below, the preparation of 3-thiazole:
5-(azetidine-1-base carbonyl)-2-bromo-4-methyl isophthalic acid, the 3-thiazole
Oxalyl chloride (0.47mL; 5.41mmol) be added to 2-bromo-4-methyl isophthalic acid, 3-thiazole-5-carboxylic acid (1.0g; 4.5mmol) in the solution in DCM (25mL).Mixture stirring at room 18 hours, and is evaporated DCM in a vacuum,, and be added to azetidine hydrochloride (503mg resistates and toluene (2 x 5mL) azeotropic; 5.41mmol) and triethylamine (2.3mL; 16.2mmol) in the solution in DCM (50mL).Mixture in stirring at room 18 hours, with DCM vacuum-evaporation, and is distributed resistates between water (75mL) and ethyl acetate (150mL).With organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained solid with the mixture wash-out of 40% ethyl acetate in isohexane, with its crystallization from ethyl acetate/isohexane, obtained required compound (490mg). 1H NMR δ (CDCl 3): 2.35 (m, 2H), 2.55 (s, 3H) and 4.1 (t, 4H).
Embodiment 35:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01522
Cesium carbonate (489mg, 1.5mmol) be added to 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide (144mg, 0.5mmol) and 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (173mg, 0.75mmol) in the solution in acetonitrile (5mL), and the mixture that will stir in microwave reactor in 120 ℃ of heating 1 hour.Mixture is cooled to room temperature and normal pressure, and acetonitrile is evaporated in a vacuum, resistates is distributed between water (25mL) and ethyl acetate (50mL).With organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (167mg) with the mixture wash-out of 75% ethyl acetate in isohexane. 1H NMR δ (CDCl 3): 1.3 (d, 6H), 2.3 (m, 2H), 2.5 (s, 3H), 4.1-4.3 (m, 4H), 4.55 (m, 1H), 6.85 (d, 1H), 7.2 (d, 1H), 7.3 (s, 1H), 8.05 (s, 1H), 8.1 (s, 1H), 8.2 (s, and 1H) 8.3 (s, 1H) and 9.45 (s, 1H) .m/z 482 (M+H) +.
Adopt similar method by 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide and suitable halogenated heterocyclic make following compound:
Figure A200810169286D01531
*This compound is crystallization from ethyl acetate and isohexane; Mpt: begin fusing at 140.4 ℃.
3-hydroxyl-5-[(1-methylethyl is described below) the oxygen base]-preparation of N-(5-methylpyrazine-2-yl) benzamide:
3-hydroxyl-5-[(1-methylethyl) oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01541
(550mg) is added to the 3-[(1-methylethyl 10% palladium on carbon) the oxygen base]-N-(5-methylpyrazine-2-yl)-5-[(phenyl methyl) the oxygen base] benzamide (5.5g, 14.6mmol) in the solution in ethanol (75mL) and THF (50mL), and mixture stirred 24 hours under hydrogen in room temperature.Remove by filter catalyzer, and be resistates,, obtained required compound (3.42g) its crystallization from ethyl acetate with the filtrate vacuum concentration.
1H NMR δ (CDCl 3): 1.3 (d, 6H), 2.5 (s, 3H), 4.5 (m, 1H), 5.8 (br, 1H), 6.5 (d, 1H), 6.9 (d, 1H), 6.95 (d, 1H), 8.05 (s, 1H), 8.4 (s, 1H) and 9.5 (s, 1H) .m/z 288 (M+H) +.
The 3-[(1-methylethyl) oxygen base]-N-(5-methylpyrazine-2-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D01542
Oxalyl chloride (2.1mL 24.0mmol) is added to the 3-[(1-methylethyl) oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (5.72g, 20.0mmol) in the solution in DCM (100mL), and with mixture in stirring at room 4 hours.Is mixture vacuum-evaporation resistates, it is dissolved among the DCM (25mL), and in 5 ℃-10 ℃ be added to 2-amino-5-methylpyrazine of stirring (2.29g, 21.0mmol) and pyridine (1.94mL is 24.0mmol) in the mixture in DCM (100mL).This mixture is evaporated to resistates to DCM in a vacuum in stirring at room 18 hours, and it is distributed between water (50mL) and ethyl acetate (150mL).With organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide, with the mixture wash-out of 30% ethyl acetate in isohexane, and crystallization from ethyl acetate/isohexane, obtained required compound (5.52g).
1H NMR δ (CDCl 3): 1.3 (d, 6H), 2.5 (s, 3H), 4.5 (m, 1H), 5.0 (s, 2H), 6.6 (s, 1H), 6.95 (s, 1H), 7.0 (s, 1H), 7.35 (m, 5H), 8.05 (s, 1H), 8.3 (s, 1H) and 9.5 (s, 1H) .m/z 378 (M+H) +.
The 3-[(1-methylethyl has been described in the front) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] preparation of phenylformic acid and 2-amino-5-methylpyrazine.
Halogenated heterocyclic 5-(azetidine-1-base carbonyl)-2 has been described in the front, the 3-dichloropyridine, 2-(azetidine-1-base carbonyl)-5-chloropyrazine, 5-(azetidine-1-base carbonyl)-2-chloropyridine and 5-(azetidine-1-base carbonyl)-2-bromo-4-methyl isophthalic acid, the preparation of 3-thiazole.
Embodiment 36:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01551
Cesium carbonate (489mg, 1.5mmol) be added to 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base-N-(5-methylpyrazine-2-yl) benzamide (159mg, 0.5mmol) and 2-(azetidine-1-base carbonyl)-5-bromopyridine (181mg, 0.75mmol) and bromine three (triphenylphosphine) copper (93mg, 0.1mmol) in the solution in DMA (5mL), and the mixture that will stir in microwave reactor in 160 ℃ of heating 4 hours.Mixture is cooled to room temperature and normal pressure, between water (75mL) and ethyl acetate (50mL), distributes, and with organic layer salt water washing, dry (MgSO 4) and vacuum-evaporation.With resistates SiO 2Chromatography purification, eluent ethyl acetate has obtained crystalline required compound from ether (45mg, 113.8 ℃ of fusing beginnings).Also the crystallization from ethyl acetate of this compound can be produced another crystalline form (132.7 ℃ of fusing beginnings). 1H NMR δ (CDCl 3): 1.3 (d, 3H), 2.3 (m, 2H), 2.5 (s, 3H), 3.3 (s, 3H), 3.45-3.55 (m, 2H), 4.2 (t, 2H), 4.55 (m, 1H), 4.65 (t, 2H), 6.75 (d, 1H), 7.05 (d, 1H), 7.25 (s, 1H), 7.3 (d, 1H), 8.0 (s, 1H), 8.05 (d, 1H), 8.25 (d, 1H), 8.3 (s, 1H) and 9.45 (s, 1H) .m/z 478 (M+H) +.
2-(azetidine-1-base carbonyl)-5-bromopyridine and 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl has been described in the front] the oxygen base }-preparation of N-(5-methylpyrazine-2-yl) benzamide.
Embodiment 37:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01561
Cesium carbonate (489mg, 1.5mmol) be added to 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide (144mg, 0.5mmol) and 2-(azetidine-1-base carbonyl)-5-bromopyridine (181mg, 0.75mmol) and bromine three (triphenylphosphine) copper (93mg, 0.1mmol) in the solution in DMA (5mL), and the mixture that will stir in microwave reactor in 160 ℃ of heating 4 hours.Mixture is cooled to room temperature and normal pressure, between water (75mL) and ethyl acetate (50mL), distributes, with organic layer salt water washing, dry (MgSO 4) and vacuum in evaporate.With resistates SiO 2Chromatography purification with the mixture wash-out of 75% ethyl acetate in isohexane, has obtained required compound (crystalline from ether) (72mg). 1H NMR δ (CDCl 3): 1.3 (d, 6H), 2.3 (m, 2H), 2.5 (s, 3H), 4.2 (t, 2H), 4.5 (m, 1H), 4.65 (t, 2H), 6.7 (s, 1H), 7.05 (s, 1H), 7.2 (s, 1H), 7.3 (d, 1H), 8.0 (br, 2H), 8.3 (br, 2H) and 9.45 (s, 1H) .m/z 448 (M+H) +.Mpt (fusing beginning) 125.7 ℃.
2-(azetidine-1-base carbonyl)-5-bromopyridine and 3-hydroxyl-5-[(1-methylethyl described in the front) the oxygen base]-preparation of N-(5-methylpyrazine-2-yl) benzamide.
Embodiment 38:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1H-pyrazole-3-yl benzamide
Figure A200810169286D01562
With 1,1-dimethyl ethyl 3-{[(3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenyl) carbonyl] amino }-1H-pyrazoles-1-manthanoate (196mg, 0.5mmol), 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (128mg, 0.55mmol) and salt of wormwood (138mg, 1.0mmol) mixture in acetonitrile (5mL) stirred 4 hours in 120 ℃ in microwave reactor.Remove in the vacuum and desolvate, and resistates is distributed between ethyl acetate and water.Organic layer is washed dry (MgSO with salt solution (50mL) 4), remove in filtration and the vacuum and desolvate, obtain brown oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, is used the mixture gradient elution of 50-100% ethyl acetate in isohexane, obtained required compound, be white foam shape thing (142mg).
1H?NMR?δ(CDCl 3)：1.26(d，3H)，2.31(quin，2H)，3.33(s，3H)，3.43-3.53(m，2H)，4.11-4.33(m，4H)，4.54(q，1H)，6.78(s，1H)，6.86(s，1H)，7.24(s，1H)，7.33(s，1H)，7.42(s，1H)，8.06(d，1H)，8.12(s，1H)，9.98(s，1H)；m/z?486(M+H) +
The front has described 1,1-dimethyl ethyl 3-{[(3-hydroxyl-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenyl) carbonyl] amino }-1H-pyrazoles-1-manthanoate and 5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine.
Embodiment 39:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-1,3-thiazol-2-yl benzamide
Figure A200810169286D01571
DIPEA (0.129mL) is added to 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid (168mg), in HATU (190mg) and the suspension of thiazolamine (40mg) in DMF (2mL), and with mixture in stirring at room 16 hours.Add entry (30mL), and mixture is extracted with ethyl acetate (3 x 20mL).With the organic extract liquid salt water washing that merges, dry (MgSO 4), and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (75mg) with the mixture wash-out of 50-100% ethyl acetate in isohexane.
1H?NMR?δ(CDCl 3)：1.27(d，3H)，2.31(quin，2H)，3.33(s，3H)，3.41-3.55(m，2H)，4.12-4.33(m，4H)，4.55(q，1H)，6.91(d，1H)，6.95(s，1H)，7.30(s，1H)，7.31(d，1H)，7.40(s，1H)，8.08(s，1H)，8.16(s，1H)；m/z503(M+H) +
Adopt similar method by 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid, 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base] phenylformic acid, 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base] phenylformic acid, 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid, 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-([(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid or 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base] phenylformic acid and suitable amino-heterocycles, make following compound.
Figure A200810169286D01581
Figure A200810169286D01601
*This reaction is to use as 1 of amino-heterocycles, and 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate carries out, and products therefrom is dissolved in acetonitrile (4mL) and the ethanol (0.5mL), and heated 12 minutes in 150 ℃ in microwave reactor.Mixture is cooled off, and vacuum concentration has obtained required product then.
+This compound is to use vapour phase hanging drop diffusion technique (vapour diffusion techniques), crystalline from ethyl acetate and isohexane.
++This compound is a crystalline from acetonitrile, 108.5 ℃ of fusing points (fusing beginning).
The front has described 1, the preparation of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.Provide 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl below] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzoic preparation:
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid
Figure A200810169286D01602
With 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base] methyl benzoate (240mg, 0.1mmol), 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (278mg, 1.2mmol) and salt of wormwood (276mg, 2.0mmol) mixture in DMA (5mL) stirred 16 hours in 120 ℃.Solution is poured in the water (30mL), used the 1N hcl acidifying, use ethyl acetate (3 x 20mL) extraction then.The organism that merges is washed dry (MgSO with salt solution (20mL) 4), remove in filtration and the vacuum and desolvate, obtained 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } methyl benzoate (only proves m/z 435 (M+H) by the LCMS data +), be brown oil (550mg).Rough 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } methyl benzoate (434mg, 1mmol) be dissolved in THF (15mL) and the methyl alcohol (5mL), add dropping water that 1N lithium hydroxide aqueous solution (2mL) continues then till obtaining clear solution.Mixture in stirring at room 4 hours, and is removed organism by evaporation.The aqueous solution is filtered, with ethyl acetate (10mL) extraction, and with water layer 2N hcl acidifying.Acidated layer is extracted with ethyl acetate (3 x 30mL), and with the organic extract liquid water (10mL), salt solution (10mL) washing that merge, and concentrate in a vacuum, obtained required compound, be canescence oily matter (375mg). 1H?NMR?δ(CDCl 3)：1.27(d，3H)，2.32(quin，2H)，3.34(s，3H)，3.42-3.55(m，2H)，4.13-4.34(m，4H)，4.54(q，1H)，6.93(s，1H)，7.40(s，1H)，7.48(s，1H)，8.08(s，1H)，8.19(s，1H)，m/z?421(M+H) +
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-Methylethyl) the oxygen base] benzoic acid, 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-Methylethyl) the oxygen base] benzoic acid and 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzoic acid is the similar method of employing, by 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-Methylethyl) oxygen base] methyl benzoate or 3-hydroxyl-5-[(1-Methylethyl) the oxygen base] methyl benzoate makes with the halogenated heterocyclic that suits.
Figure A200810169286D01611
Figure A200810169286D01621
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl is described below] the oxygen base }-the 5-[(1-methylethyl) the oxygen base] benzoic preparation:
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base] phenylformic acid
With 3-hydroxyl-5-[(1-methylethyl) the oxygen base] methyl benzoate (0.84g, 4mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (1.16g, 4.8mmol), cesium carbonate (3.92g, 1.2mmol) and bromine three (triphenylphosphine) copper (I) (744mg, 0.8mmol) mixture in DMA (20mL) stirred 6 hours in 160 ℃ in microwave reactor.With mixture water (100mL) dilution, with ethyl acetate (2 x 20mL) washing, water layer is filtered, use the 2N hcl acidifying then.Acidic layer with ethyl acetate (3 x 50mL) extraction, is washed organism water (2 x 20mL), the salt solution (20mL) that merges dry (MgSO 4), and remove in a vacuum and desolvate.Resistates is developed with ether, obtained required compound, be white solid (1.06g). 1H?NMR?δ(d 6-DMSO)：1.28(d，6H)，2.28(quin，2H)，4.08(t，2H)，4.59(t，2H)，4.69(quin，1H)，6.98(t，1H)，7.10(s，1H)，7.28(s，1H)，7.56(d，1H)，7.99(d，1H)，8.41(s，1H)；m/z?357(M+H) +
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid is to adopt similar method, by 2-(azetidine-1-base carbonyl)-5-bromopyridine and 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base] methyl benzoate makes.
Figure A200810169286D01631
3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base has been described in the front] methyl benzoate, methyl 3-hydroxyl-5-[(1-methylethyl) the oxygen base] preparation of methyl benzoate and suitable halogenated heterocyclic.
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-1H-pyrazole-3-yl benzamide, embodiment 39k, also be to adopt following method, by 1,1-dimethyl ethyl 3-[({3-hydroxyl-5-[(1-methylethyl) the oxygen base] phenyl } carbonyl) amino]-1H-pyrazoles-1-manthanoate preparation.
1,1-dimethyl ethyl 3-[({3-hydroxyl-5-[(1-methylethyl) the oxygen base] phenyl } carbonyl) amino]-(56.3g 0.16mol) is dissolved in the acetonitrile (500mL) 1H-pyrazoles-1-manthanoate, and places the 3L fixed container.Adding salt of wormwood-325 order-(64.5g, 0.47mol), (33.5g 0.17mol), carries out the charging flushing with the 100mL acetonitrile to add 2-(the basic carbonyl of azetidine-1-)-5-chloropyrazine then.Under nitrogen, mixture is stirred and is heated to 60 ℃ fast.Add acetonitrile (250mL) again, after 1.5 hours, add DMF (150mL) with dissolving resistates solid material.To react to stir and spend the night, be cooled to room temperature and filtration then.The filtrate vacuum concentration removing acetonitrile, and is under agitation poured into residual solution in the water (1500mL).Precipitated solid is filtered, be dissolved among the DCM (560mL), with 1:1 salt solution/saturated sodium bicarbonate aqueous solution (2 x 500mL) washing and dry (MgSO 4).Organic solution is filtered and TFA (50mL).With solution in stirring at room 3 hours.Add TFA (50mL) again, and mixture was stirred other 3 hours in 30 ℃, then in stirred overnight at room temperature.Remove under the vacuum and desolvate, and with resistates and methylbenzene azeotropic.Orange oily resistates is dissolved in the ethyl acetate (500mL), and washs, dry (MgSO with saturated sodium bicarbonate solution (2 x 500mL), salt solution (500mL) 4) and concentrate, obtain light yellow waxy solid (64g).With this solid in 45 ℃ with ethyl acetate (200mL) development 2 hours, filter, with the ethyl acetate washing, and in vacuum chamber in 40 ℃ of dried overnight, obtained white solid (52g).Thick solid product is carried out the column chromatography purifying on silicon-dioxide, the mixture of methanol solution in DCM with 7N ammonia carries out wash-out (0.5-6.5% methyl alcohol gradient), obtained pure material requested [pure products 1] (23.6g, HPLC shows purity 99.1%) and the impure material (34.5g, HPLC show purity 82.8%) that from mix fraction and aforesaid liquid, reclaims.In DCM (50mL), and crystallization goes out white solid impure material dissolves.Solids removed by filtration, and, obtained pure material requested [pure products 2] (6.5g, HPLC show purity 98.3%) with the DCM washing.Filtrate is carried out the column chromatography purifying on silicon oxide, the mixture wash-out (0-5% methyl alcohol gradient) of methanol solution in DCM with 7N ammonia obtained material requested [pure products 3] (18.3g, HPLC show purity 98.7%).Pure products 1,2 and 3 is merged (48.4g) and be loaded in the 3L fixed container.(500mL 10vol) is added in the mixture, and mixture under agitation is heated to backflow, and (400mL 8vol), and removes remaining solid by heat filtering to add ethyl acetate again ethyl acetate.Filtrate is cooled to 60 ℃, and drips isohexane (250mL).With about 1 hour slurries are cooled to 20 ℃, then in stirred overnight at room temperature.Filter this slurries, and with solid with isohexane (2 x 200mL) washing, then in vacuum chamber in 40 ℃ of dryings, obtain white solid (34.5g).With this solid in vacuum chamber in 60 ℃ of further dried overnight, obtain required compound, be white powder (33.1g).It is and different crystalline form described above 113.8 ℃ of fusing points (fusing beginning).
The preparation of 2-(azetidine-1-base carbonyl)-5-chloropyrazine has been described in the front.
Describe below 1,1-dimethyl ethyl 3-[({3-hydroxyl-5-[(1-methylethyl) the oxygen base] phenyl } carbonyl) amino]-preparation of 1H-pyrazoles-1-manthanoate.
1,1-dimethyl ethyl 3-[({3-hydroxyl-5-[(1-methylethyl) the oxygen base] phenyl } carbonyl) amino]-1H-pyrazoles-1-manthanoate
Figure A200810169286D01641
1,1-dimethyl ethyl 3-[({3-[(1-methylethyl) oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenyl } carbonyl) amino]-1H-pyrazoles-1-manthanoate (76.4g, 0.17mol) be dissolved in ethanol (750mL, 10vol) in, and add 10% palladium on carbon (water-moistened) (7.5g).With mixture hydrogenation 18 hours under 25 ℃ and 5bar pressure.By
Figure A200810169286D0164181642QIETU
Remove by filter catalyzer, and filter cake is washed with methyl alcohol (250mL).The filtrate that merges is concentrated under vacuum, obtained light brown spumescence solid, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 10-70% ethyl acetate in isohexane, has been obtained material requested, be white foam shape solid (56.4g). 1H?NMR?δ(CDCl 3)：1.30(d，6H)，1.64(s，9H)，4.49-4.55(m，1H)，6.67(t，1H)，6.98(d，2H)，7.10(d，1H)，8.02(d，1H)，9.21(s，1H)；m/z?360(M-H) -
1,1-dimethyl ethyl 3-[({3-[(1-methylethyl) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenyl } carbonyl) amino]-1H-pyrazoles-1-manthanoate
Figure A200810169286D01651
Oxalyl chloride (76mL, 0.87mol) drips of solution in DCM (125mL) is added to the 3-[(1-methylethyl) oxygen base]-the 5-[(phenyl methyl) the oxygen base] (50g is 0.17mol) and in the slurries of DMF (1mL) in DCM (300mL) for phenylformic acid.To react on stirring at room 2 hours, and under vacuum, remove then and desolvate, and with resistates and toluene (200mL) azeotropic.The oily resistates is dissolved in the anhydrous pyridine (100mL), and under nitrogen this mixture was added in 1 with 5 minutes, (38.4g is 0.21mol) in the solution in anhydrous pyridine (325mL) for 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.To react on stirring at room 1 hour, and remove under the vacuum and desolvate, and with resistates and methylbenzene azeotropic.Resistates is distributed between DCM (500mL) and water (500mL), and organic layer is washed and dry (MgSO with saturated sodium bicarbonate aqueous solution (500mL), salt solution (500mL) 4).Organic solution is concentrated under vacuum, and, obtained orange arborescens thing (88.8g) resistates and methylbenzene azeotropic twice.With this arborescens thing chromatography purification on silicon-dioxide, with the mixture wash-out of 25-50% ethyl acetate in isohexane, obtained material requested, be light yellow foam (76.4g). 1H?NMR?δ(CDCl 3)：1.33(d，6H)，1.56(s，9H)，4.49-4.55(m，1H)，5.04(s，2H)，6.69(t，1H)，7.00(t，1H)，7.06-7.07(m，1H)，7.17(d，1H)，7.32-7.45(m，5H)，8.04(d，1H)，9.48(s，1H)；m/z?450(M-H) -
The 3-[(1-methylethyl has been described in the front) the oxygen base]-the 5-[(phenyl methyl) the oxygen base] phenylformic acid and 1, the preparation process of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.
Embodiment 40:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1,5-dimethyl-1H-pyrazole-3-yl)-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide
Figure A200810169286D01661
With N-(1,5-dimethyl-1H-pyrazole-3-yl)-the 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } solution of benzamide (0.62mmol) in acetonitrile (5mL) salt of wormwood (1.24mmol) and 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (0.68mmol) is handled, in microwave reactor, be heated to then 120 ℃ 4.5 hours.With mixture vacuum-evaporation, between DCM (20mL) and water (20mL), distribute then.With organism vacuum-evaporation, chromatography purification on silicon-dioxide with the mixture wash-out of 0-10% methyl alcohol in ethyl acetate, has obtained required compound then, is white foam shape thing (160mg).
1H?NMR?δ(d 6-DMSO)：1.25(d，3H)，2.24(s，3H)，2.26(m，2H)，3.30(s，3H)，3.50(m，2H)，3.66(s，3H)，4.05(t，2H)，4.37(t，2H)，4.78(m，1H)，6.42(s，1H)，7.03(t，1H)，7.38(t，1H)，7.51(t，1H)，8.23(d，1H)，8.34(d，1H)，10.76(s，1H)；m/z?514，516(M+H) +
5-(azetidine-1-base carbonyl)-2, the description of 3-dichloropyridine have been described in the front.
N-(1,5-dimethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl is described below] the oxygen base } preparation of benzamide:
N-(1,5-dimethyl-1H-pyrazole-3-yl)-3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzamide
Figure A200810169286D01662
With N-(1,5-dimethyl-1H-pyrazole-3-yl)-3-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base-the 5-[(phenyl methyl) the oxygen base] solution of benzamide (2.57mmol) in ethanol (40mL) under argon gas with 10% palladium on carbon (100mg, moistening) processing with 0.1mL water and 1mL ethanol.Flask is vacuumized and fill, should operate and repeat 4 times with hydrogen.This mixture stirred under hydrogen in room temperature spend the night, will react then and pass through
Figure A200810169286D01663
Filter, and filter cake is washed with ethanol (50mL) and ethyl acetate (50mL).The organic layer that merges is evaporated in a vacuum, obtained required compound, be white foam shape thing (597mg). 1HNMR?δ(d 6-DMSO)：1.23(d，3H)，2.25(s，3H)，3.31(s，3H)，3.47(m，2H)，3.66(s，3H)，4.66(m，1H)，6.40(s，1H)，6.46(t，1H)，6.95(t，1H)，7.06(t，1H)，9.60(s，1H)，10.49(s，1H)；m/z?320(M+H) +
N-(1,5-dimethyl-1H-pyrazole-3-yl)-3-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] benzamide
With 3-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } solution of phenylformic acid (4.11mmol) in DMF (10mL) is with 1,5-dimethyl-1H-pyrazole-3-yl amine (4.52mmol), DIPEA (5.93mmol) and HATU (3.85mmol) handle, and stir under argon gas in room temperature and to spend the night.Mixture is poured in the water (100mL), and extracted with ethyl acetate (3 x 100mL).Organic layer with salt solution (20mL) washing and vacuum-evaporation, then at the enterprising circumstances in which people get things ready for a trip spectrometry of silicon-dioxide purifying, with the mixture wash-out of 20-80% ethyl acetate in isohexane, has been obtained required compound, be yellow oil (1.05g).
1H?NMR?δ(d 6-DMSO)：1.23(d，3H)，2.25(s，3H)，3.30(s，3H)，3.48(m，2H)，3.67(s，3H)，4.71(m，1H)，5.16(s，2H)，6.42(s，1H)，6.73(t，1H)，7.20(t，1H)，7.26(t，1H)，7.34(m，1H)，7.41(m，2H)，7.46(m，2H)，10.62(s，1H)；m/z?410(M+H) +
3-[(1S has been described in the front)-2-methoxyl group-(1-methylethyl) oxygen base]-the 5-{[phenyl methyl] the oxygen base } benzoic preparation.
1,5-dimethyl-1H-pyrazole-3-yl amine is to prepare according to literature method [J.Het.Chem., (1982), 19,1267].
Embodiment 41:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(1,5-dimethyl-1H-pyrazole-3-yl)-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } benzamide
Figure A200810169286D01681
With N-(1,5-dimethyl-1H-pyrazole-3-yl)-and 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-solution of 5-hydroxybenzamide (0.61mmol) in acetonitrile (5mL) salt of wormwood (1.23mmol) and 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (0.68mmol) is handled, in microwave reactor, be heated to then 120 ℃ 4 hours.Mixture is filtered and vacuum-evaporation,,, obtained required compound, be white foam shape thing (175mg) with the mixture wash-out of 0-5% methyl alcohol in ethyl acetate then at the enterprising circumstances in which people get things ready for a trip spectrometry of silicon-dioxide purifying.
1H?NMR?δ(d 6-DMSO)：2.27(m，5H)，3.66(s，3H)，4.06(t，2H)，4.37(t，2H)，4.65(m，1H)，4.73(m，1H)，4.77(m，1H)，4.85(m，1H)，5.10(m，1H)，6.42(s，1H)，7.16(t，1H)，7.45(t，1H)，7.60(t，1H)，8.23(d，1H)，8.35(d，1H)，10.71(s，1H)m/z?520(M+H) +
5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine have been described in the front.
N-(1,5-dimethyl-1H-pyrazole-3-yl)-3-{[2-fluoro-1-(methyl fluoride) ethyl is described below] the oxygen base }-preparation of 5-hydroxybenzamide:
N-(1,5-dimethyl-1H-pyrazole-3-yl)-3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-hydroxybenzamide
Figure A200810169286D01682
With N-(1,5-dimethyl-1H-pyrazole-3-yl)-and 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] solution of benzamide (1.89mmol) in ethanol (30mL) is with 10% palladium on carbon (78mg, moistening with 1mL ethanol) processing.Flask is vacuumized and fill, and should operate and repeat 4 times again with hydrogen.Mixture stirred under hydrogen in room temperature spend the night, then mixture is passed through
Figure A200810169286D01683
Filter, and filter cake is washed with ethanol (50mL) and ethyl acetate (50mL).Organism vacuum-evaporation with merging has obtained required compound, is white foam shape thing (613mg).
1H?NMR?δ(d 6-DMSO)：2.52(s，3H)，3.66(s，3H)，4.66(m，2H)，4.77(m，2H)，4.97(m，1H)，6.41(s，1H)，6.56(t，1H)，7.02(m，1H)，7.15(t，1H)，10.52(s，1H)；m/z?326(M+H) +
N-(1,5-dimethyl-1H-pyrazole-3-yl)-3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D01691
3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] solution of phenylformic acid (2.96mmol) in DMF (5mL) is with 1,5-dimethyl-1H-pyrazole-3-yl amine (3.11mmol), DIPEA (5.93mmol) and HATU (3.85mmol) handle, and in stirred overnight at room temperature.Mixture is poured in the water (100mL), and extracted with ethyl acetate (3 x 75mL).With organism salt water washing, dry (MgSO 4) and vacuum-evaporation, then at the enterprising circumstances in which people get things ready for a trip spectrometry of silicon-dioxide purifying,, obtained required compound with the mixture wash-out of 50-100% ethyl acetate in isohexane, be flint glass shape thing (784mg). 1H?NMR?δ(d 6-DMSO)：2.81(s，3H)，3.67(s，3H)，4.66(m，2H)，4.78(m，2H)，5.04(m，1H)，5.69(s，2H)，6.43(s，1H)，6.85(t，1H)，7.28(m，1H)，7.32(m，1H)，7.35(m，1H)，7.41(m，2H)，7.47(m，2H)，10.64(s，1H)；m/z?416(M+H) +
3-{[2-fluoro-1-(methyl fluoride) ethyl has been described in the front] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid and 1, the preparation of 5-dimethyl-1H-pyrazole-3-yl amine.
Embodiment 42:3-{[6-(azetidine-1-base carbonyl) pyridazine-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide
Figure A200810169286D01692
With 3-(azetidine-1-base carbonyl)-6-chlorine pyridazine (45mg, 0.23mmol), 3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base]-N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide (70mg, 0.23mmol) and salt of wormwood (63mg, 0.46mmol) mixture in acetonitrile (3mL) stirred 1 hour in 130 ℃ in Smith Creator microwave reactor.Cross the elimination solid, and remove in the vacuum and desolvate.By anti-phase preparation HPLC purifying, (+mixture wash-out in 0.2%TFA) is used methanol-eluted fractions to usefulness 5-95% acetonitrile, obtains required product, is colorless solid (97mg) at water with resistates. 1H?NMR?δ(CDCl 3)：1.34(3H，d)，1.66(3H，s)，2.33-2.41(2H，m)，3.40(3H，s)，3.48-3.52(1H，m)，3.56-3.60(1H，m)，3.79(3H，s)，4.27(2H，d)，4.58-4.62(1H，m)，4.76(2H，t)，6.79(1H，d)，6.97(1H，t)，7.25-7.29(3H，m)，7.35-7.36(1H，m)，8.26-8.28(1H，m)，8.49(1H，s)；m/z?467(M+H) +.
3-hydroxyl-5-[(1S)-2-methoxyl group-(1-methylethyl) oxygen base has been described in the front]-preparation of N-(1-methyl isophthalic acid H-pyrazole-3-yl) benzamide.
The preparation of 3-(azetidine-1-base carbonyl)-6-chlorine pyridazine is described below:
3-(azetidine-1-base carbonyl)-6-chlorine pyridazine
Figure A200810169286D01701
Phosphoryl chloride (50mL) be added to 6-hydroxyl pyridazine-3-carboxylic acid (9.82g, 70.05mmol) in, and gained solution is heated to backflow.After 45 minutes, with the solution cooling, and excessive phosphoryl chloride is removed in decompression.THF (50mL) is added in the black residue, and gained solution is cooled to 0 ℃.Drip triethylamine (9.77mL, 70.05mmol) and azetidine (4.0g, 70.05mmol).The gained mixture heating up to room temperature, and is stirred and to spend the night.Decompression is removed volatile matter and is added entry (50mL), with sodium hydroxide solution resistates is adjusted to pH8 then.With water layer ethyl acetate extraction five times, with the organic layer drying (MgSO that merges 4), and remove and desolvate, obtained crude product, be the dark oil thing.Sample by preparation HPLC purifying, with the mixture wash-out of 5-95% acetonitrile in water (+0.2% TFA), has been obtained this title compound, be colorless solid (135mg).
1H?NMR?δ(CDCl 3)：2.39-2.47(2H，m)，4.30(2H，t)，4.82(2H，t)，7.64(1H，d)，8.21(1H，d)；m/z?198(M+H) +.
Embodiment 43:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(4-methyl isophthalic acid, 3-thiazol-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01711
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid (200mg, 0.52mmol), HATU (475mg, 1.25mmol) and 2-amino-4-methylthiazol (119mg 1.04mmol) is suspended among the DMF (2mL).Add DIPEA (0.220mL, 1.26mmol), and with reaction mixture in stirring at room 16 hours.Remove DMF in the vacuum and add entry (15mL).With mixture with ethyl acetate (3 x, 20 mL) extraction, united extraction liquid, and water (15mL), 1N hydrochloric acid (10mL), water (10mL), saturated sodium bicarbonate aqueous solution (10mL), salt solution (10mL) washing, dry (MgSO 4), filter and vacuum-evaporation.With resistates SiO 2Chromatography purification with the mixture gradient elution of 60-100% ethyl acetate in isohexane, has obtained required compound (70mg).
1H?NMR?δ(CDCl 3)：2.13-2.29(m，2H)，2.32(s，3H)，2.34-2.42(m，2H)，3.89-4.02(m，4H)，4.26(t，2H)，4.69(t，2H)，4.96-4.99(m，1H)，6.57(d，1H)，6.94(t，1H)，7.29(t，1H)，7.33(t，1H)，8.35(d，1H)，8.85(d，1H)，9.72(s，1H)；m/z?482(M+H) +，480(M-H) -
Adopt similar method by 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid and suitable amino-heterocycles make following compound.
Figure A200810169286D01712
*This reaction is to be used as 1 of amino-heterocycles, and 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate carries out, be dissolved in products therefrom in the acetonitrile and in microwave reactor in 150 ℃ the heating 5-10 minute.With the mixture cooling, vacuum concentration has obtained required product then.The front has described 1, the preparation of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl is described below] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzoic preparation:
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[(3S)-tetrahydrofuran (THF)-3- Base oxygen base] phenylformic acid
Figure A200810169286D01731
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate (7.02mmol) is dissolved among the THF (50mL), add 1N sodium hydroxide (7.0mL), that continues adds entry (50mL), and with gained solution in stirring at room 4 hours.Remove organism in the vacuum, the aqueous solution is filtered, and extract with ethyl acetate (30mL).With water layer 2N hcl acidifying, with ethyl acetate (3 x 50mL) extraction, and with the washing of organic extract water (10mL), salt solution (10mL), be evaporated to driedly then, obtained material requested, be white foam shape thing (2.33g). 1H?NMR?δ(CDCl 3)：2.14-2.30(m，2H)，2.34-2.42(m，2H)，3.89-3.94(m，2H)，3.97-4.02(m，2H)，4.28(t，2H)，4.69(t，2H)，4.97-5.00(m，1H)，6.94(t，1H)，7.48(t，2H)，8.34(d，1H)，8.85(d，1H)；m/z?386(M+H) +，384(M-H) -
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate
Figure A200810169286D01732
With 3-hydroxyl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate (2.5g, 10.5mmol), salt of wormwood (2.9g, 21.0mmol) and 2-(the basic carbonyl of azetidine-1-)-(2.48g, 12.6mmol) solution in DMA (25mL) was in 120 ℃ of heating 2 hours for the 5-chloropyrazine.Solution is diluted water (3 x 50mL), salt solution (20mL) washing, dry (MgSO with ethyl acetate (150mL) 4), removing in filtration and the vacuum and desolvate, resistates is by chromatography purification on silicon-dioxide, and with the mixture wash-out of 0-50% ethyl acetate in isohexane, having obtained required compound is colorless oil (2.8g). 1H?NMR?δ(CDCl 3)：2.13-2.29(m，2H)，2.34-2.41(m，2H)，3.88-4.04(m，4H)，3.90(s，3H)，4.25(t，2H)，4.68(t，2H)，4.96-5.00(m，1H)，6.91(t，1H)，7.43(d，2H)，8.32(d，1H)，8.85(d，1H)；m/z?386(M+H) +，384(M-H) -
The 3-hydroxyl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate
Figure A200810169286D01741
The 3-[(phenyl methyl) the oxygen base]-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] (25.0g 76.2mmol) is dissolved in THF (150mL) and the ethanol (150mL) methyl benzoate.Add 10% palladium on carbon (30mg), and place mixture under the hydrogen and in stirring at room till reaction is finished.Remove catalyzer by diatomite filtration, and, obtained orange, place post crystallization the filtrate vacuum concentration.With solid filtering and with the ether washing, obtained required product, be white solid (13.75g).
1H?NMR?δ(CDCl 3)：2.1-2.3(2H，m)，3.9(3H，s)，3.9-3.95(2H，m)，3.97-4.05(2H，m)，4.95(1H，s)，5.6(1)，6.6(1H，t)，7.1(1H，t)，7.13(1H，t)；m/z?237(M+H) +
The 3-[(phenyl methyl) oxygen base]-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate
With 3-hydroxyl-5-{[phenyl methyl] the oxygen base } methyl benzoate (18.8g, 72.75mmol), (3R)-tetrahydrofuran (THF)-3-base 4-toluene sulfonic acide ester (18.5g, 76.4mmol) and salt of wormwood (20.08g, 145.5mmol) mixture in butyronitrile (250mL) in 130 ℃ the heating 3 hours.Remove in the vacuum and desolvate, and add ethyl acetate.With organism water (40mL), 0.5M sodium hydroxide solution (40mL), salt solution (40mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, with resistates SiO 2Chromatography purification with the mixture gradient elution of 0-5% methyl alcohol in DCM, has obtained required compound, is colorless oil (20.1g). 1H?NMR?δ(CDCl 3)：2.08-2.26(m，2H)，3.78-4.01(m，4H)，3.90(s，3H)，4.92-4.96(m，1H)，5.08(s，2H)，6.69(t，1H)，7.15(t，1H)，7.29(t，1H)，7.34-7.44(m，5H)；m/z?327(M+H) +
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate and (3R)-tetrahydrofuran (THF)-3-base 4-toluene sulfonic acide ester.
Embodiment 44:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-N-(4-methyl isophthalic acid, 3-thiazol-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01751
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.70mL, 0.53mmol) be added to 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid (200mg, 0.48mmol) in the solution in DCM (6mL), and in stirring at room 2 hours.Add 2-amino-4-methylthiazol (110mg, 0.96mmol) and pyridine (0.078mL 0.96mmol), and will react on stirring at room 16 hours.Remove in the vacuum and desolvate, add entry (20mL), and mixture is extracted with ethyl acetate (3 x 20mL).Extraction liquid is merged, with 2N hydrochloric acid (20mL), saturated sodium bicarbonate aqueous solution (20mL), water (20mL), salt solution (20mL) washing, dry (MgSO 4), filter, and evaporate in the vacuum.With resistates SiO 2Chromatography purification with the mixture gradient elution of 50-100% ethyl acetate in isohexane, has obtained required compound (78mg).
1H?NMR?δ(CDCl 3)：2.13-2.28(m，2H)，2.31(s，3H)，2.35-2.43(m，2H)，3.88-4.02(m，4H)，4.21-4.28(m，2H)，4.31-4.39(m，2H)，4.96-5.00(m，1H)，6.56(d，1H)，6.94(t，1H)，7.28(t，1H)，7.34(t，1H)，8.16(d，1H)，8.25(d，1H)，9.83(s，1H)；m/z?515(M+H) +，513(M-H) -
Adopt similar method by 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] the suitable amino-heterocycles of phenylformic acid makes following compound:
*This reaction is to use as 1 of amino-heterocycles, and 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate carries out, and products therefrom is dissolved in the acetonitrile, and heated 5-10 minute in 150 ℃ in microwave reactor.Mixture is cooled off, and vacuum concentration has obtained required product then.The front has described 1, the preparation of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl is described below] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzoic preparation:
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid
Figure A200810169286D01771
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate (3.75g, 8.68mmol) be dissolved among the THF (40mL), add 1N sodium hydroxide (8.68mL, 8.68mmol), that continues adds entry (40mL) and methyl alcohol (1), and with gained solution in stirring at room 3 hours.Remove organism in the vacuum, the aqueous solution is filtered and extract with ethyl acetate (30mL).Water layer 2N hcl acidifying with ethyl acetate (3x50mL) extraction, and with the washing of organic extract liquid water (10mL), salt solution (10mL), is evaporated to driedly then, has obtained material requested, is white foam shape thing (3.53g).
1H?NMR?δ(CDCl 3)：2.14-2.30(m，2H)，2.35-2.43(m，2H)，3.89-3.94(m，2H)，3.96-4.02(m，2H)，4.22-4.38(m，4H)，4.97-5.01(m，1H)，6.95(t，1H)，7.46-7.47(m，1H)，7.49-7.50(m，1H)，8.15(d，1H)，8.26(d，1H)；m/z419(M+H) +，417(M-H) -.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate
Figure A200810169286D01772
With 3-hydroxyl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate (2.5g, 10.5mmol), salt of wormwood (2.9g, 21.0mmol) and 5-(azetidine-1-base carbonyl)-2, (2.9g, 12.6mmol) solution in DMA (25mL) was in 120 ℃ of heating 5 hours for the 3-dichloropyridine.This solution is diluted water (3 x 50mL), salt solution (20mL) washing, dry (MgSO with ethyl acetate (150mL) 4), remove in filtration and the vacuum and desolvate.By chromatography purification on silicon-dioxide, with the mixture wash-out of 20-50% ethyl acetate in isohexane, having obtained required compound is colorless oil (3.75g) with resistates. 1H?NMR?δ(CDCl 3)：2.13-2.29(m，2H)，2.38(quin，2H)，3.85-4.04(m，4H)，3.90(s，3H)，4.19-4.37(m，4H)，4.96-5.00(m，1H)，6.91(t，1H)，7.42-7.45(m，2H)，8.14(d，1H)，8.25(d，1H)；m/z?433(M+H) +.
3-hydroxyl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base has been described in the front] methyl benzoate and 5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine.
Embodiment 45:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01781
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.133mL, 1.0mmol) be added to 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid (350mg, 0.94mmol) in the suspension in DCM (10mL), and in stirring at room 30 minutes.Add 2-amino-5-methylpyrazine (196mg, 1.82mmol) and pyridine (0.148mL 1.82mmol), and will react on stirring at room 2 hours.Remove in the vacuum and desolvate, and add entry (30mL).Mixture with ethyl acetate (3 x 20mL) extraction, is washed with 2N hydrochloric acid (20mL), water (10mL), saturated sodium bicarbonate aqueous solution (20mL), water (10mL) and salt solution (20mL).With the organic extract liquid drying (MgSO that merges 4), filter and vacuum concentration.Resistates by chromatography purification, with the mixture wash-out of 0-10% methyl alcohol in DCM, is obtained required product, be colorless oil (150mg). 1H?NMR?δ(CDCl 3)：2.12-2.18(1H，m)，2.22-2.29(1H，m)，2.31-2.40(2H，m)，2.56(3H，s)，3.89-4.03(4H，m)，4.25(2H，t)，4.71(2H，t)，4.97-5.00(1H，m)，6.76(1H，t)，7.15(1H，t)，7.25(1H，d)，7.38-7.40(1H，m)，8.12(1H，s)，8.14(1H，d)，8.32(1H，s)，8.34(1H，d)，9.52(1H，d)；m/z?476(M+H) +，474(M-H) -
Adopt similar method by 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid or 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid and suitable amino-heterocycles make following compound.
Figure A200810169286D01791
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl has been described in the front] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzoic preparation.
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl is described below] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzoic preparation:
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid
Figure A200810169286D01801
3-hydroxyl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] methyl benzoate (875mg, 3.68mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (1.06g, 4.41mmol), cesium carbonate (3.59g, 11.03mmol) and bromine three (triphenylphosphine) copper (0.69g, 0.735mmol) be added to together among the DMA (16mL), and in microwave reactor, heated 9 hours in 160 ℃.This compound is dissolved in the water (25mL), with ethyl acetate (2 x 20mL) washing.Water layer with 1N hydrochloric acid (30mL) acidifying, and is extracted with ethyl acetate (5 x 30mL).With organic extract liquid water (30mL), salt solution (30mL) washing that merges, dry (MgSO 4) washing, filter and evaporation, obtained brown solid, it is developed with ether, obtained required compound, be beige solid (820mg).
1H?NMR?δ(d 6-DMSO)：2.00-2.06(m，1H)，2.23-2.37(m，3H)，3.78-3.95(m，4H)，4.13(t，2H)，4.64(t，2H)，5.16-5.19(m，1H)，7.05(t，1H)，7.18-7.19(m，1H)，7.33-7.34(m，1H)，7.62(dd，1H)，8.04(d，1H)，8.46(d，1H)；m/z?385(M+H) +，383(M-H) -
3-hydroxyl-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base has been described in the front] preparation of methyl benzoate and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Embodiment 46:3-{[3-chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01802
Cesium carbonate (782mg, 2.4mmol) be added to 3-hydroxyl-5-[(1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide (230mg, 0.8mmol) and 2,3-two chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine (234mg, 0.96mmol) in the solution in acetonitrile (5mL), and the mixture that will stir in microwave reactor in 120 ℃ of heating 2 hours.This mixture is cooled to room temperature, and acetonitrile is evaporated in a vacuum.Resistates is distributed between water (25mL) and ethyl acetate (50mL), with organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (290mg) with the mixture wash-out of 75% ethyl acetate in isohexane.
1H NMR δ (CDCl 3): 1.3 (d, 6H), 1.9 (m, 4H), 2.5 (s, 3H), 3.4-3.7 (m, 4H), 4.55 (m, 1H), 6.85 (d, 1H), 7.2 (d, 1H), 7.25 (d, 1H), 7.9 (d, 1H), 8.1 (dd, 1H), 8.2 (s, 1H), 8.35 (s, 1H) and 9.45 (s, 1H); M/z 496 (M+H) +.
Adopt similar method by 2,3-two chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine and 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide or 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide makes following compound.
Figure A200810169286D01811
Describe below 2, the preparation of 3-two chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine:
2,3-two chloro-5-(tetramethyleneimine-1-base carbonyl) pyridine
Figure A200810169286D01812
Oxalyl chloride (3.2mL; 36.0mmol) be added to 5,6 dichloro-nicotinic acid (5.76g; 30.0mmol) is with diox (7.5mL; 30.0mmol) in the solution of 4M hydrogenchloride in DCM (50mL) in.This mixture evaporates DCM in a vacuum in stirring at room 18 hours, with resistates and toluene (2x15mL) azeotropic, is added to tetramethyleneimine (3.0mL then; 36.0mmol) and triethylamine (10.0mL; 72mmol) in the solution in DCM (150mL).This mixture, distributes resistates DCM vacuum-evaporation in stirring at room 6 hours between water (75mL) and ethyl acetate (100mL), with organic layer salt water washing, and dry (MgSO 4) and vacuum-evaporation.With resistates SiO 2Chromatography purification with the mixture wash-out of 50% ethyl acetate in isohexane, has obtained required compound, and it is further purified by crystallization from ethyl acetate and isohexane (6.88g). 1H NMR δ (CDCl 3): 1.9 (m, 4H), 3.4 (m, 2H), 3.6 (s, 3H), 7.9 (s, 1H) and 8.4 (s, 1H); M/z 245 (M+H) +.
3-hydroxyl-5-[(1-methylethyl has been described in the front) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide and 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-preparation of N-(5-methylpyrazine-2-yl) benzamide.
Embodiment 47:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide
Figure A200810169286D01821
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide (209mg, 0.66mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (159mg, 0.80mmol) and salt of wormwood (184mg 1.33mmol) dissolves/is suspended in the acetonitrile (3.5mL).Reaction mixture was heated cooling then, filtration and vacuum concentration 4 hours in 120 ℃ in microwave reactor.Crude product by chromatography purification on silicon-dioxide, with the mixture wash-out of 0-5% methyl alcohol in DCM, is obtained required product, be white foam shape thing (39mg). 1H?NMR?δ(d 6-DMSO)：1.57-1.68(m，2H)，1.97-2.05(m，2H)，2.26-2.36(m，2H)，3.47-3.55(m，2H)，3.78(s，3H)，3.84-3.91(m，2H)，4.10(t，2H)，4.57(t，2H)，4.67-4.75(m，1H)，6.58(d，1H)，7.11(t，1H)，7.43(t，1H)，7.54(t，1H)，7.60(d，1H)，8.56(d，1H)，8.68(d，1H)，10.80(s，1H)；m/z?479(M+H)+
Adopt similar method to make following compound with suitable halogenated heterocyclic by 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide or 3-hydroxy-n-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide.
Figure A200810169286D01831
2-(azetidine-1-base carbonyl)-5-chloropyrazine and 5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine have been described in the front.
The preparation of 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide is described below:
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide
Figure A200810169286D01841
N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3-[(phenyl methyl) the oxygen base]-(1.85g 4.54mmol) is dissolved in the ethanol (40mL) benzamide 5-(tetrahydrochysene-2H-pyrans-4-base oxygen base), and adds 10% palladium on carbon (97mg) under argon gas.To be reflected under the hydrogen and stir 16 hours, pass through then
Figure A200810169286D0184183905QIETU
Filter, and, obtained required compound, be light brown solid (1.30g) the filtrate vacuum concentration.
1H?NMR?δ(d 6-DMSO)：1.54-1.65(m，2H)，1.94-2.03(m，2H)，3.44-3.54(m，2H)，3.78(s，3H)，3.83-3.90(m，2H)，4.56-4.65(m，1H)，6.51(t，1H)，6.56(d，1H)，6.97(t，1H)，7.08(t，1H)，7.59(d，1H)，9.63(s，1H)，10.59(s，1H)；m/z?318(M+H) +
N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3-[(phenyl methyl) oxygen base]-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide
The 3-[(phenyl methyl) the oxygen base]-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) phenylformic acid (3.14g, 9.5mmol), 1-methyl isophthalic acid H-pyrazoles-3-amine (1.85g, 19.0mmol) and HATU (4.70g, 12.35mmol) be dissolved among the DMF (12.5mL), and adding DIPEA (3.31mL, 19.0mmol).The gained mixture was in stirring at room 20 hours.This mixture water (150mL) stopped reaction with ethyl acetate (2 x 75mL) extraction, is used the salt water washing, dry (MgSO 4) and concentrate in a vacuum.With resistates SiO 2Chromatography purification with the mixture wash-out of 0-50% ethyl acetate in isohexane, has obtained required compound, is light yellow gum (1.85g).
The 3-[(phenyl methyl) oxygen base]-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) phenylformic acid
Figure A200810169286D01843
The 3-[(phenyl methyl) the oxygen base]-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) methyl benzoate (9.00g, 26.3mmol) be dissolved in THF (120mL) and the methyl alcohol (30mL), and adding lithium hydroxide monohydrate (3.24g, 78.9mmol) solution in water (60mL).This biphasic solution in stirring at room 3 hours up to LCMS show finish conversion till.Remove organism in the vacuum, and adding and water (40mL).By adding the pH regulator to 7 of hydrochloric acid with mixture, the gained throw out is filtered and use cold water washing, obtained required product, be white solid (8.03g). 1H?NMR?δ(d 6-DMSO)：1.57(m，2H)，1.94(m，2H)，3.50(m，2H)，3.84(m，2H)，4.62(m，1H)，5.15(s，2H)，6.87(t，1H)，7.09(m，1H)，7.14(m，1H)，7.34(t，1H)，7.40(t，2H)，7.46(d，2H)，12.99(s，1H)；m/z?327(M+H) +
The 3-[(phenyl methyl) oxygen base]-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) methyl benzoate
Figure A200810169286D01851
Under argon gas with 3-hydroxyl-5-{[phenyl methyl] the oxygen base methyl benzoate (7.75g, 30.0mmol), tetrahydrochysene-4H-pyrans-4-alcohol (4.28mL, 45.0mmol) and triphenylphosphine (11.8g, 45.0mmol) stirring in THF (166mL), and in ice bath, be cooled to 5 ℃.(19.6mL 45.0mmol) is added drop-wise in this mixture, simultaneously internal temperature is remained on below 10 ℃ DEAD.Stirring was carried out 16 hours,, and resistates is dissolved in ethyl acetate (60mL) and the isohexane (60mL) then with the reaction mixture vacuum concentration.Disgorging, with the filtrate vacuum concentration, by at the enterprising circumstances in which people get things ready for a trip spectrometry of silicon-dioxide purifying, with mixture (DCM that on a small quantity the be used for auxiliary column load) wash-out of 0-25% ethyl acetate in isohexane, obtained required compound, be colorless oil (9.0g).
1H?NMR?δ(d 6-DMSO)：1.58(m，2H)，1.94(m，2H)，3.49(m，2H)，3.84(m，5H)，4.65(m，1H)，5.16(s，2H)，6.92(t，1H)，7.10(m，1H)，7.15(m，1H)，7.34(t，1H)，7.40(t，2H)，7.46(d，2H)；m/z?343(M+H) +
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate.The preparation of 3-hydroxy-n-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide is described below:
3-hydroxy-n-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide
Figure A200810169286D01861
N-(5-methylpyrazine-2-yl)-3-[(phenyl methyl) the oxygen base]-(1.70g 4.05mmol) is dissolved in the ethanol (40mL) benzamide 5-(tetrahydrochysene-2H-pyrans-4-base oxygen base), and adds 10% palladium on carbon (86mg) catalyzer under argon gas.To be reflected under the hydrogen and stir 4 days, and filter then, and, obtained required compound, be cream-colored solid (1.20g) .m/z 330 (M+H) the filtrate vacuum concentration +
N-(5-methylpyrazine-2-yl)-3-[(phenyl methyl) oxygen base]-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide
Figure A200810169286D01862
The 3-[(phenyl methyl) the oxygen base]-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) phenylformic acid (3.14g, 9.5mmol), 2-amino-5 methylpyrazine (2.08g, 19.0mmol) and HATU (4.70g, 12.35mmol) be dissolved among the DMF (12.5mL), and adding DIPEA (3.31mL, 19.0mmol).The gained mixture was in stirring at room 20 hours.This mixture water (150mL) is ended, and, used the salt water washing, dry (MgSO with ethyl acetate (2 x 75mL) extraction 4) and concentrate in a vacuum.With resistates SiO 2Chromatography purification with the mixture wash-out of 0-50% ethyl acetate in isohexane, has obtained required compound, is light brown gum (1.70g).
m/z?420(M+H) +
The 3-[(phenyl methyl has been described in the front) the oxygen base]-preparation of 5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) phenylformic acid and 2-amino-5 methylpyrazine.
Embodiment 48:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide
Figure A200810169286D01871
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide (200mg, 0.63mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (184mg, 0.76mmol), bromine three (triphenylphosphine) copper (186mg, 0.2mmol) and cesium carbonate (411mg 1.26mmol) dissolves/is suspended among the DMA (3.5mL).Reaction mixture was heated cooling then, filtration and vacuum concentration 1 hour in 200 ℃ in microwave reactor.Add entry (25mL), and product is extracted with ethyl acetate (2 x 25mL), with resistates drying (MgSO 4), then at the enterprising circumstances in which people get things ready for a trip spectrometry of silicon-dioxide purifying,, obtained required compound with the mixture wash-out of 0-10% methyl alcohol in DCM, be light yellow foam (65mg). 1H?NMR?δ(d 6-DMSO)：1.57-1.67(m，2H)，1.97-2.05(m，2H)，2.23-2.34(m，2H)，3.51(t，2H)，3.78(s，3H)，3.83-3.90(m，2H)，4.08(t，2H)，4.59(t，2H)，4.69-4.76(m，1H)，6.56(d，1H)，7.00(t，1H)，7.29(t，1H)，7.49(t，1H)，7.54-7.58(m，1H)，7.60(d，1H)，8.00(d，1H)，8.41(d，1H)，10.83(s，1H)；m/z?478(M+H) +
Adopt similar method to make following compound by 2-(azetidine-1-base carbonyl)-5-bromopyridine and 3-hydroxy-n-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide.
Figure A200810169286D01872
The preparation of 2-(azetidine-1-base carbonyl)-5-bromopyridine, 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide and 3-hydroxy-n-(5-methylpyrazine-2-yl)-5-(tetrahydrochysene-2H-pyrans-4-base oxygen base) benzamide has been described in the front.
Embodiment 49:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3R)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01881
With 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3R)-tetrahydrofuran (THF)-3-base oxygen base] benzamide (150mg, 0.49mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (120mg, 0.49mmol), bromine three (triphenylphosphine) copper (92mg, 0.10mmol) and cesium carbonate (484mg, 1.48mmol) mixture in acetonitrile (7mL) stirred 6 hours in 160 ℃ in microwave reactor.With solid filtering, remove in the vacuum and desolvate, and ethyl acetate (50mL) is added in the resistates.With organic phase water (10mL), salt solution (20mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, with the resistates chromatography purification,, obtained this title compound with the mixture gradient elution of 0-10% methyl alcohol in DCM, be white solid (86mg). 1H?NMR?δ(CDCl 3)：2.10-2.17(m，1H)，2.19-2.25(m，1H)，2.32-2.38(m，2H)，3.76(s，3H)，3.87-3.92(m，1H)，3.95-4.01(m，3H)，4.24(t，2H)，4.71(t，2H)，4.97(t，1H)，6.73(t，1H)，6.81(s，1H)，7.11(s，1H)，7.24(s，1H)，7.27-7.30(m，1H)，7.36-7.39(m，1H)，8.12(d，1H)，8.31(d，1H)，8.72(s，1H)；m/z?464(M+H) +.
The preparation of 2-(azetidine-1-base carbonyl)-5-bromopyridine has been described in the front.
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3R)-tetrahydrofuran (THF)-3-base oxygen base is described below] preparation of benzamide:
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3R)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01882
With 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl) the oxygen base] benzamide (450mg, 1.39mmol), (3S)-tetrahydrofuran (THF)-3-base 4-toluene sulfonic acide ester (507mg, 2.09mmol) and salt of wormwood (481mg 3.48mmol) stirred 3 hours in 160 ℃ in microwave reactor at the suspension of acetonitrile (5mL).Remove in the vacuum and desolvate, and add ethyl acetate.With organism water (40mL), salt solution (40mL) washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate.With resistates SiO 2Chromatography purification with the mixture gradient elution of 0-100% ethyl acetate in isohexane, has obtained N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3-[(phenyl methyl) the oxygen base]-5-[(3R)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide, be white foam shape thing.It is dissolved in the ethanol (5mL), and disposable adding ammonium formiate (182mg, 2.88mmol).To react with the argon gas covering, and add 10% palladium carbon (30mg).This mixture in 140 ℃ of heating 10 minutes, filters catalyzer, and removes volatile matter in a vacuum in microwave reactor, has obtained this title compound, is white solid (305mg).
1H?NMR?δ(d 6-DMSO)：1.96-2.00(m，1H)，2.20-2.25(m，1H)，3.74-3.85(m，6H)，3.87-3.91(m，1H)，5.06-5.08(m，1H)，6.46(t，1H)，6.57(d，1H)，6.97(s，1H)，7.02(s，1H)，7.60(d，1H)，9.74(s，1H)，10.70(s，1H)；m/z?304(M+H) +.
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(phenyl methyl has been described in the front) the oxygen base] preparation of benzamide.As described in example 25 above, adopt the method that is similar to preparation (3R)-tetrahydrofuran (THF)-3-base 4-toluene sulfonic acide ester, make (3S)-tetrahydrofuran (THF)-3-base 4-toluene sulfonic acide ester.
Embodiment 50:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-pyridine-2-yl-benzamide
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.080mL, 0.6mmol) be added to 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid (193mg, 0.50mmol) in the solution in DCM (10mL), and in stirring at room 30 minutes.Add the 2-aminopyridine (57mg, 0.6mmol) and pyridine (0.082mL 1mmol), and will react restir 16 hours.Remove in the vacuum and desolvate, and resistates is dissolved in the ethyl acetate (50mL).With organic phase water (2 x 20mL), 1N citric acid (10mL), water (10mL), saturated sodium bicarbonate aqueous solution (10mL) and salt solution (100mL) washing, dry then (MgSO 4), filter, and vacuum concentration.With resistates SiO 2Chromatography purification with the mixture gradient elution of 50-80% ethyl acetate in isohexane, has obtained required compound, is white foam shape thing (42mg). 1H?NMR?δ(CDCl 3)：1.28(d，3H)，2.31(quin，2H)，3.34(s，3H)，3.43-3.56(m，2H)，4.19(t，2H)，4.57(q，1H)，4.62(t，2H)，6.90(s，1H)，7.03(t，1H)，7.20(s，1H)，7.36(s，1H)，7.71(t，1H)，8.23(d，1H)，8.28(s，1H)，8.30(d，1H)，8.64(s，1H)，8.79(s，1H)；m/z?464(M+H) +
Adopt similar method to make following compound by suitable phenylformic acid and suitable amino-heterocycles.
Figure A200810169286D01901
Figure A200810169286D01911
*Behind initial chromatography purification, isolating sample contains about 15% unknown impuritie.
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl has been described in the front] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid, 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base] phenylformic acid, 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid, 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-the 5-[(1-methylethyl) the oxygen base] phenylformic acid and 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzoic preparation.
Embodiment 51:3-{[3-chloro-5-(morpholine-4-base carbonyl) pyridine-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01912
Cesium carbonate (489mg, 1.5mmol) be added to 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide (152mg, 0.5mmol) and 4-[(5,6-dichloropyridine-3-yl) carbonyl] morpholine (143mg, 0.55mmol) in the solution in acetonitrile (5mL), and in microwave reactor in 120 ℃ of heating 2 hours.Acetonitrile is removed in evaporation in the vacuum, and resistates is distributed between water (25mL) and ethyl acetate (50mL).With organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide, use eluent ethyl acetate, obtained required compound (203mg). 1H?NMR?δ(CDCl 3)：2.05-2.2(m，2H)，3.4-3.7(m，8H)，3.7(s，3H)，3.85(m，2H)，3.95(d，2H)，4.9(m，1H)，6.7(d，1H)，6.8(d，1H)，7.15(d，1H)，7.25(d，2H)，7.85(d，1H)，8.0(d，1H)，8.95(s，1H)；m/z?512(M+H) +.
Adopt similar method by 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide and 7-[(5,6-dichloropyridine-3-yl) carbonyl]-7-azabicyclo [2.2.1] heptane makes following compound:
Figure A200810169286D01921
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base has been described in the front] preparation of benzamide.
4-[(5 is described below, 6-dichloropyridine-3-yl) carbonyl] preparation of morpholine:
4-[(5,6-dichloropyridine-3-yl) carbonyl] morpholine
Figure A200810169286D01922
Oxalyl chloride (3.2mL; 36.0mmol) be added to 5,6 dichloro-nicotinic acid (5.76g; 30.0mmol) and 4M hydrogenchloride De diox (7.5mL; 30mmol) in the solution of solution in DCM (50mL).With mixture in stirring at room 18 hours, with DCM vacuum-evaporation, resistates and toluene (2x 15mL) azeotropic, and be added to morpholine (3.1mL; 36.0mmol) and triethylamine (10.0mL; 72mmol) in the solution in DCM (150mL).Mixture in stirring at room 18 hours, with DCM vacuum-evaporation, and is distributed resistates between water (75mL) and ethyl acetate (100mL).With organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained required compound (7.1g) with the mixture wash-out of 50% ethyl acetate in isohexane.
1H?NMR?δ(CDCl 3)：3.3-3.8(br，8H)，7.8(s，1H)，8.25(s，1H)；m/z?261(M+H) +.
7-[(5 is described below, 6-dichloropyridine-3-yl) carbonyl]-preparation of 7-azabicyclo [2.2.1] heptane:
7-[(5,6-dichloropyridine-3-yl) carbonyl]-7-azabicyclo [2.2.1] heptane
Figure A200810169286D01931
Oxalyl chloride (3.2mL; 36.0mmol) (5.76g is 30.0mmol) with 4M hydrogenchloride De diox (7.5mL to be added to 5,6 dichloro-nicotinic acids; 30.0mmol) in the solution of solution in DCM (50mL).With mixture in stirring at room 18 hours, with DCM vacuum-evaporation, resistates and toluene (2 x 15mL) azeotropic, and be added to 7-azabicyclo [2.2.1] heptane hydrochloride (4.75g; 36.0mmol) and triethylamine (15.1mL; 108mmol) in the solution in DCM (150mL).With mixture in stirring at room 18 hours, DCM vacuum-evaporation, and resistates distributed between water (75mL) and ethyl acetate (150mL).Organic layer salt water washing, dry (MgSO 4) and evaporation, obtained solid, with its crystallization from ethyl acetate, obtained required compound (5.45g).
1H?NMR?δ(CDCl 3)：1.5(m，4H)，1.85(m，4H)，4.0(br，1H)，4.65(br，1H)，7.9(s，1H)，8.4(s，1H)；m/z?271(M+H) +.
Embodiment 52:3-{[5-(azetidine-1-base carbonyl)-4-methyl isophthalic acid, the 3-thiazol-2-yl] the oxygen base }-N-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D01941
Cesium carbonate (489mg, 1.5mmol) be added to 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide (152mg, 0.5mmol) and 5-(azetidine-1-base carbonyl)-2-bromo-4-methyl isophthalic acid, 3-thiazole (144mg, 0.55mmol) in the solution in acetonitrile (5mL), and the compound that will stir in microwave reactor in 120 ℃ of heating 2 hours.With mixture cooling,, and resistates distributed between water (25mL) and ethyl acetate (50mL) acetonitrile vacuum-evaporation.Organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide, use eluent ethyl acetate, obtained required compound, be solid, with its further crystallization (122mg) from ethyl acetate/isohexane mixture.
1H NMR δ (CDCl 3): 2.0-2.15 (m, 2H), 2.2-2.3 (m, 2H), 2.5 (s, 3H), 3.75 (s, 3H), 3.85 (m, 2H), 3.95 (d, 2H), 4.1-4.3 (m, 4H), 4.9 (m, 1H), 6.75 (d, 1H), 6.95 (d, 1H), 7.25 (d, 2H), 7.3 (d, 1H) and 8.8 (s, 1H); M/z 484 (M+H) +.
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base has been described in the front] benzamide and 5-(azetidine-1-base carbonyl)-2-bromo-4-methyl isophthalic acid, the preparation of 3-thiazole.
Embodiment 53:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01942
1M hydrochloric acid (5mL) is added to 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide (82mg, 0.14mmol) in the solution in methyl alcohol (5mL), and in stirring at room 1 hour.Remove methyl alcohol in the vacuum, and resistates is adjusted to pH6, be extracted in the ethyl acetate (3 x 50mL), and the organism that merges is washed with salt solution (50mL), dry (MgSO 4), filter and vacuum concentration.Should thick yellow oil chromatography purification on silicon-dioxide, with the mixture wash-out of 0-5% methyl alcohol in ethyl acetate, obtained required compound, be white foam (36mg).Can be with crystallization in the mixture of this compound from t-butyl methyl ether and methyl alcohol. 1H?NMR?δ(CDCl 3)：1.24(d，3H)，1.81(s，1H)，2.28(quin，2H)，2.48(s，3H)，3.67-3.72(m，2H)，4.17(t，2H)，4.47-4.54(m，1H)，4.63(t，2H)，6.73(t，1H)，7.09(t，1H)，7.25(t，1H)，7.27-7.30(m，1H)，8.01-8.06(m，2H)，8.24(d，1H)，8.58(s，1H)，9.44(d，1H)；m/z?464(M+H) +
Adopt similar method by 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide makes following compound.
Figure A200810169286D01951
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl is described below] the oxygen base }-5-[((1S)-2-{[(1, and the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-preparation of N-(5-methylpyrazine-2-yl) benzamide.
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01952
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.11mL, 0.80mmol) be added to 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base] phenylformic acid (0.3g, 0.62mmol) in the solution in DCM (10mL), and stirred 1 hour.Add 2-amino-5-methylpyrazine (135mg, 1.23mmol), add then pyridine (0.1mL, 1.23mmol), and with mixture restir 30 minutes, vacuum concentration, and distribution between ethyl acetate (50mL) and water (50mL) then.Water layer further is extracted in the ethyl acetate (50mL), and with the organism water (50mL), salt solution (50mL) washing that merge, dry (MgSO 4), filter and vacuum concentration.Should thick oily matter chromatography purification on silicon-dioxide, with the mixture wash-out of 40-100% ethyl acetate in isohexane, obtained required compound, be colorless oil (82mg).
1H?NMR?δ(CDCl 3)：0.00(s，3H)，0.03(s，3H)，0.83(s，9H)，1.28(d，3H)，2.32(quin，2H)，2.53(s，3H)，3.64-3.77(m，2H)，4.22(t，2H)，4.47-4.51(m，1H)，4.68(t，2H)，6.81(t，1H)，7.10(t，1H)，7.27(t，1H)，7.33-7.35(m，1H)，8.08-8.11(m，2H)，8.30(d，1H)，8.37(s，1H)，9.50(d，1H)；m/z?578(M+H) +
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide is to adopt similar method, by 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base] the phenylformic acid preparation.
Figure A200810169286D01961
The preparation of 2-amino-5-methylpyrazine has been described in the front.
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl is described below] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base] benzoic preparation.
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-diformazan The base ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base] phenylformic acid
Figure A200810169286D01971
Tert-butyldimethylsilyl chloride (1.11g, 7.36mmol) be added to 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base } phenylformic acid (1.37g, 3.68mmol) and 1,8-diazabicyclo (5,4,0) (1.11mL is 7.36mmol) in the mixture in acetonitrile (20mL), and in stirring at room 24 hours for 11 carbon-7-alkene.Remove in the vacuum and desolvate, and water (80mL) is added in the gained oily matter.Mixture is adjusted to pH10 with the 1M sodium hydrogen carbonate solution, uses ether (80mL) washing then to remove impurity.Water layer is adjusted to pH3 with the 1M citric acid, is extracted in the ethyl acetate (2 x 100mL), and wash the organic phase that merges, dry (MgSO with salt solution (50mL) 4), filter and vacuum concentration.With this roughage chromatogram purification on Isolute NH2 tube, use the mixture wash-out of ammonia in methyl alcohol then with methyl alcohol, obtained cream-coloured foam.This material subsequently with the ethyl acetate development, has been obtained desired mixt, be cream-coloured foam (1.0g). 1H?NMR?δ(CDCl 3)：0.01(s，3H)，0.03(s，3H)，0.83(s，9H)，1.27(d，3H)，2.26-2.35(m，2H)，3.58-3.79(m，2H)，4.15-4.25(m，2H)，4.43-4.49(m，1H)，4.60-4.69(m，2H)，6.77-6.82(m，1H)，7.28(m，2H)，7.42-7.44(m，1H)，8.02-8.07(m，1H)，8.27-8.29(m，1H)；m/z?487(M+H) +
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base } phenylformic acid
Figure A200810169286D01972
With 3-hydroxyl-5-[(1S)-2-hydroxyl-1-methyl ethoxy] methyl benzoate (2.35g, 10.39mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (2.51g, 10.39mmol), cesium carbonate (10.16g, 31.16mmol) and bromine three (triphenylphosphine) copper (1.93g, 2.08mmol) mixture in DMA (20mL) stirred 8 hours in 160 ℃ in microwave reactor.Mixture is distributed in ethyl acetate (50mL) and water (50mL).With water layer 1M citric acid acidifying, and be extracted in the ethyl acetate (2 x 100mL), use the salt water washing, dry (MgSO 4) and vacuum in remove and to desolvate.Should thick oily matter chromatography purification on Isolute NH2 tube, use the mixture wash-out of ammonia in methyl alcohol then with methyl alcohol, obtained material requested, be cream-coloured foam (1.37g) .m/z 373 (M+H) +
3-hydroxyl-5-[(1S)-2-hydroxyl-1-methyl ethoxy has been described in the front] preparation of methyl benzoate and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Embodiment 54:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01981
1M hydrochloric acid (10mL) is added to 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide (180mg, 0.29mmol) in the solution in methyl alcohol (10mL), and in stirring at room 1 hour.Remove methyl alcohol in the vacuum, and resistates is adjusted to pH6, be extracted into then in the ethyl acetate (3 x 50mL).The organism that merges is washed dry (MgSO with salt solution (50mL) 4), and vacuum concentration, obtained yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 0-5% methyl alcohol in ethyl acetate, has been obtained required compound, be white foam shape thing (134mg).With compound crystallization from ethyl acetate and methyl alcohol.
1H?NMR?δ(CDCl 3)：1.33(d，3H)，2.40(quin，2H)，2.56(s，3H)，3.76-3.81(m，2H)，4.25(t，2H)，4.38(t，2H)，4.57-4.64(m，1H)，6.98(t，1H)，7.31(t，1H)，7.42(t，1H)，8.14(s，1H)，8.17(d，1H)，8.26(d，1H)，8.64(s，1H)，9.55(s，1H)；m/z?498(M+H) +
Following compound is to adopt similar method preparation:
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl is described below] the oxygen base }-5-[((1S)-2-{[(1, and the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-preparation of N-(5-methylpyrazine-2-yl) benzamide.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D01992
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.1mL, 0.75mmol) be added to 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base] phenylformic acid (0.3g, 0.58mmol) in the solution in DCM (10mL), and stirred 1 hour.Successively add 2-amino-5-methylpyrazine (126mg, 1.15mmol) and pyridine (0.094mL, 1.15mmol), and with mixture restir 30 minutes, vacuum concentration, and distribution between ethyl acetate (50mL) and water (50mL) then.Water layer further is extracted in the ethyl acetate (50mL), and with the organism water (50mL), salt solution (50mL) washing that merge, dry (MgSO 4), and vacuum concentration.Should thick oily matter chromatogram purification on silicon-dioxide, with the mixture wash-out of 40-100% ethyl acetate in isohexane, obtained this title compound, be oily matter (180mg).
1H?NMR?δ(CDCl 3)：0.00(s，3H)，0.03(s，3H)，0.82(s，9H)，1.28(d，3H)，2.35(quin，2H)，2.51(s，3H)，3.63-3.78(m，2H)，4.19(t，2H)，4.32(t，2H)，4.47-4.51(m，1H)，6.95(t，1H)，7.23-7.24(m，1H)，7.35(t，1H)，8.08(s，1H)，8.12(d，1H)，8.21(d，1H)，8.45(s，1H)，9.51(d，1H)；m/z?612(M+H) +
The following intermediate that uses in the preparation in embodiment 54a-c is to adopt similar method preparation.
Figure A200810169286D02001
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl is described below] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base] benzoic preparation.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base] phenylformic acid
Figure A200810169286D02011
Tert-butyldimethylsilyl chloride (2.78g, 18.4mmol) be added to 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base } phenylformic acid (3.74g, 9.19mmol) and 1,8-diazabicyclo (5,4,0) (2.75mL is 7.36mmol) in the mixture in acetonitrile (40mL), and in stirring at room 24 hours for 11 carbon-7-alkene.Remove in the vacuum and desolvate, and water (80mL) is added in the gained oily matter.Mixture is adjusted to pH10 with the 1M sodium hydrogen carbonate solution, and washs to remove any impurity with ether.Water layer is adjusted to pH3 with the 1M citric acid, and is extracted in the ethyl acetate (2 x 100mL).The organism that merges is washed dry (MgSO with salt solution (50mL) 4), filter and vacuum concentration, obtained required compound, be white foam shape thing (2.9g).
1H?NMR?δ(CDCl 3)：0.00(s，3H)，0.03(s，3H)，0.83(s，9H)，1.28(d，3H)，2.35(quin，2H)，3.62-3.78(m，2H)，4.21(t，1H)，4.32(t，1H)，4.43-4.50(m，1H)，6.95-6.98(m，1H)，7.42-7.45(m，1H)，7.50-7.53(m，1H)，8.12(d，1H)，8.22(d，1H)；m/z?521(M+H) +
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base } phenylformic acid
Figure A200810169286D02012
Lithium hydroxide monohydrate (529mg, 12.59mmol) solution in water (25mL) is added to 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base } methyl benzoate (5.3g, 12.6mmol) in the solution in THF (50mL), and in stirring at room 3 hours.Remove THF in the vacuum, and water layer is washed to remove any impurity with ethyl acetate (50mL).With the water layer acidifying, and be extracted in the ethyl acetate (2 x 50mL), and with organism salt water washing, dry (MgSO 4), and vacuum concentration, obtained required compound, be white foam shape thing (3.74g).
1H?NMR?δ(CDCl 3)：1.24(d，3H)，2.31(quin，2H)，3.65-3.72(m，2H)，4.17(t，2H)，4.29(t，2H)，4.47-4.54(m，1H)，6.93(t，1H)，7.40(s，1H)，7.46(s，1H)，8.08(d，1H)，8.19(d，1H)；m/z?407(M+H) +
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base } methyl benzoate
Figure A200810169286D02021
With 3-hydroxyl-5-[(1S)-2-hydroxyl-1-methyl ethoxy] methyl benzoate (2.35g, 10.39mmol), 5-(azetidine-1-base carbonyl)-2,3-dichloropyridine (2.41g, 10.39mmol) and salt of wormwood (2.87g, 20.8mmol) mixture in acetonitrile (20mL) stirred 6 hours in 160 ℃ in microwave reactor.Remove in the vacuum and desolvate, and resistates is distributed between ethyl acetate (50mL) and water (50mL).Organic layer is washed dry (MgSO with salt solution (50mL) 4), and remove in a vacuum and desolvate.Should thick oily matter chromatogram purification on silicon-dioxide, with the mixture wash-out of 30-100% ethyl acetate in isohexane, obtained required compound, be yellow oil (5.3g).
1H?NMR?δ(CDCl 3)：1.24(d，3H)，1.94(t，1H)，2.32(quin，2H)，3.65-3.72(m，2H)，3.83(s，3H)，4.16(t，2H)，4.28(t，2H)，4.47-4.54(m，1H)，6.90(t，1H)，7.37-7.38(m，1H)，7.43-7.45(m，1H)，8.08(d，1H)，8.18(d，1H)；m/z?421(M+H) +
3-hydroxyl-5-[(1S)-2-hydroxyl-1-methyl ethoxy has been described in the front] methyl benzoate and 5-(azetidine-1-base carbonyl)-2, the preparation of 3-dichloropyridine.
Embodiment 55:3-{[5-(azetidine-1-base carbonyl) pyridine-2-yl] the oxygen base }-5-{[(1S)-2- Hydroxyl-1-methylethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide
With 3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide (237mg, 0.77mmol), 5-(azetidine-1-base carbonyl)-2-chloropyridine (151mg, 0.77mmol) and salt of wormwood (213mg, 1.54mmol) mixture in acetonitrile (5mL) stirred 5 hours in 140 ℃ in microwave reactor, and stirred 10 hours in 160 ℃.With the mixture vacuum concentration, and add ethyl acetate (50mL).With mixture water (50mL), salt solution (50mL) washing, dry (MgSO 4), and vacuum concentration, obtain brown oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 0-10% methyl alcohol in ethyl acetate, has been obtained required compound, be white foam shape thing (64mg).
1H?NMR?δ(CDCl 3)：1.29(d，3H)，2.38(quin，2H)，2.49(s，3H)，2.72(s，1H)，3.75-3.77(m，2H)，4.20-4.41(m，4H)，4.51-4.59(m，1H)，6.95(t，1H)，6.99(d，1H)，7.35(t，1H)，7.37-7.38(m，1H)，8.05-8.08(m，1H)，8.40(d，1H)，11.08(s，1H)；m/z?470(M+H) +
The preparation of 5-(azetidine-1-base carbonyl)-2-chloropyridine has been described in the front.
3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl is described below] the oxygen base }-preparation of N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide.
The 3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl] the oxygen base }-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-5-yl) benzamide
Figure A200810169286D02032
Iodine trimethyl silane (5.51mL, 38.7mmol) be added to 3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base-N-(3-methyl isophthalic acid, 2,4-thiadiazoles-2-yl) benzamide (2.5g, 7.73mmol) in the mixture in acetonitrile (25mL), and will react on stirring at room 48 hours.Add methyl alcohol (15mL), and will react and stir 1 hour, add saturated sodium thiosulfate solution (10mL) then, and stirred 20 minutes.Remove the volatility thing in the vacuum, and aqueous residue is extracted in the ethyl acetate (2 x 150mL).With organism water, salt water washing, dry (MgSO 4), and vacuum concentration, obtained yellow oil.This solid successively with DCM and ethyl acetate development, has been obtained required compound, be white solid (1.44g). 1H?NMR?δ(d 6-DMSO)：1.23(d，3H)，2.49(s，3H)，3.46-3.59(m，2H)，4.48-4.52(m，1H)，4.89(t，1H)，6.60(s，1H)，7.08(s，1H)，7.24(s，1H)，9.91(s，1H)，13.28(s，1H)；m/z?310(M+H) +
3-hydroxyl-5-{[(1S)-2-methoxyl group-(1-methylethyl) oxygen base has been described in the front }-preparation of N-(3-methyl isophthalic acid, 2,4-thiadiazoles-2-yl) benzamide.
Embodiment 56:N-(1-methyl isophthalic acid H-pyrazole-3-yl)-3-{[6-(tetramethyleneimine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D02041
Cupric iodide (I) (95mg, 0.5mmol), 2,2,6,6-tetramethyl--3,5-heptane diketone (0.42mL, 2.0mmol) and cesium carbonate (489mg 1.5mmol) is added to 3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] (152mg is 0.5mmol) with 5-bromo-2-(tetramethyleneimine-1-base carbonyl) pyridine (141mg for benzamide, 0.55mmol) in the solution in NMP (5mL), and in microwave reactor in 160 ℃ of stirring heating mixtures 12 hours.This mixture is cooled to room temperature and normal pressure, and mixture is distributed between water (75mL) and ethyl acetate (50mL), with organic layer salt water washing, dry (MgSO 4) and be evaporated to resistates, with its chromatography purification on silicon-dioxide, use eluent ethyl acetate, obtained material requested (81mg).
1H?NMR?δ(CDCl 3)：1.8-1.95(m，4H)，2.0-2.2(m，2H)，3.6(m，2H)，3.75(s，3H)，3.85(m，2H)，3.95(m，2H)，4.9(m，1H)，6.65(m，1H)，6.75(m，1H)，7.1(m，1H)，7.2(s，1H)，7.25(m，1H)，7.3(dd，1H)，7.8(d，1H)，8.25(d，1H)，8.8(s，1H)；m/z?478(M+H) +.
3-hydroxy-n-(1-methyl isophthalic acid H-pyrazole-3-yl)-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base has been described in the front] preparation of benzamide.
The preparation of 5-bromo-2-(tetramethyleneimine-1-base carbonyl) pyridine is described below.
5-bromo-2-(tetramethyleneimine-1-base carbonyl) pyridine
Oxalyl chloride (0.83mL; 9.3mmol) and DMF (1drop) be added to 5-bromine isonicotinic acid (1.57g; 7.75mmol) and 4M hydrogenchloride De diox (1.93mL; 7.75mmol) in the solution of mixture in DCM (50mL).Mixture in stirring at room 18 hours, is removed DCM in the vacuum, and with resistates and toluene (2 x 15mL) azeotropic.Resistates is added to tetramethyleneimine (0.78mL; 9.3mmol) and triethylamine (2.6mL; 18.6mmol) in the solution in DCM (40mL), and with mixture in stirring at room 6 hours.Remove DCM in the vacuum, and resistates is distributed between water (75mL) and ethyl acetate (100mL), with organic layer salt water washing, dry (MgSO 4) and be evaporated to resistates, with its chromatography purification on silicon-dioxide,, obtained material requested (1.40g) with the mixture wash-out of 50% ethyl acetate in isohexane. 1H?NMR?δ(CDCl 3)：1.85(m，4H)，3.6(m，2H)，3.7(s，3H)，7.7(d，1H)，7.85(s，1H)，8.55(s，1H)；m/z?257(M+H) +.
Embodiment 57:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-pyrazine-2-base-5-[(3S)-tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D02052
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.095mL, 0.75mmol) be added to 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid (250mg, 0.65mmol) in the solution in DCM (6mL), and in stirring at room 30-40 minute.Add the amino pyrazine of 2-(125mg, 1.31mmol) and pyridine (0.106mL 1.31mmol), and will react on stirring at room 2 hours.Remove in the vacuum and desolvate, add entry (20mL), and mixture is extracted with ethyl acetate (3 x 20mL).Organic extract liquid is merged, and wash, dry (MgSO with 2N hydrochloric acid (20mL), saturated sodium bicarbonate solution (20mL), water (20mL), salt solution (20mL) 4) and vacuum-evaporation.With crude product chromatogram purification on silicon-dioxide,, obtained required compound (71mg) with the mixture gradient elution of 0-10% methyl alcohol in DCM.
1H?NMR?δ(CDCl 3)：2.13-2.30(m，2H)，2.32-2.39(m，2H)，3.89-4.04(m，4H)，4.25(t，2H)，4.71(t，2H)，4.97-5.01(m，1H)，6.77(t，1H)，7.16(t，1H)，7.25(s，1H)，7.40(dd，1H)，8.14(d，1H)，8.28(dd，1H)，8.34(d，1H)，8.38(s，1H)，8.40(d，1H)，9.66(d，1H)；m/z?462(M+H) +，460(M-H) -
Adopt similar method, by 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid and 1,1-dimethyl ethyl 3-amino-5-methyl isophthalic acid H-pyrazoles-1-manthanoate or 1,1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate makes following compound.The material dissolves that makes in acetonitrile (3mL), and in microwave reactor in 150 ℃ the heating 10 minutes.Behind the chromatography purification, obtained material requested.
Figure A200810169286D02061
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl has been described in the front] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid and 1, the preparation of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.
Describe below 1, the preparation of 1-dimethyl ethyl 3-amino-5-methyl isophthalic acid H-pyrazoles-1-manthanoate.
1,1-dimethyl ethyl 3-amino-5-methyl isophthalic acid H-pyrazoles-1-manthanoate
Figure A200810169286D02062
(800mg 8.25mmol) is dissolved among the DMF (10mL) in 0 ℃, and (336mg 8.25mmol) handles, and then stirred 30 minutes with sodium hydride 5-methyl isophthalic acid H-pyrazoles-3-amine.(1.80g 8.25mmol), is heated to room temperature with reaction, and restir 1 hour with slowly adding warmed-up tert-Butyl dicarbonate in 5 minutes via syringe then.Reaction is dissolved in saturated sodium bicarbonate aqueous solution (50mL) and the ethyl acetate (100mL).Organic layer is separated dry then (MgSO 4), filter and evaporation.By the column chromatography purifying, with the mixture wash-out of 50-100% ethyl acetate in isohexane, obtained this title compound, be colorless oil (380mg).
1H?NMR?δ(CDCl 3)：1.62(s，9H)，2.43(s，3H)，3.87(br.s，2H)，5.60(s，1H)
Embodiment 58:3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D02071
1-chloro-N, N-2-trimethylammonium propenyl amine (0.08mL, 0.60mmol) be added to 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } phenylformic acid (215mg, 0.5mmol) in the solution in DCM (5mL), and will react on stirring at room 30-40 minute.Add pyridine (0.08mL, 1.0mmol) and 2-amino-5-methylpyrazine (108mg 1.0mmol), and will react stirring 16 hours, vacuum concentration then, and add entry (20mL).Mixture with ethyl acetate (3 x 20mL) extraction, is washed dry (MgSO with 1N hydrochloric acid (20mL), saturated sodium bicarbonate solution (20mL), salt solution (20mL) 4) and concentrate in a vacuum.With crude product chromatography purification on silicon-dioxide, with the mixture gradient elution of 0-10% methyl alcohol in DCM, obtained required compound, be white solid (150mg). 1H NMR δ (CDCl 3): 2.40 (quintet, 2H), 2.56 (s, 3H), 4.22-4.31 (m, 2H), and 4.32-4.40 (m, 2H), 4.62-4.82 (m, 5H), 7.06 (t, 1H), 7.38 (t, 1H), 7.47 (t, 1H), 8.15 (s, 1H), 8.17 (s, 1H), 8.25 (d, 1H), 8.46 (s, 1H), 9.55 (s, 1H); M/z 518 (M+H) +, 516 (M-H) -
Adopt similar method, by suitable phenylformic acid and 2-amino-5-methylpyrazine, 1,1-dimethyl ethyl 3-amino-5-methyl isophthalic acid H-pyrazoles-1-manthanoate or 1,1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate makes following compound.Using 1,1-dimethyl ethyl 3-amino-5-methyl isophthalic acid H-pyrazoles-1-manthanoate or 1 under the situation of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate, needs other step to obtain material requested.Described other step need a person with the qualifications of a general the material be dissolved in the acetonitrile (3mL) and in microwave reactor in 150 ℃ the heating 10 minutes, behind chromatogram purification, separate material requested then.
Figure A200810169286D02081
The front has described 1,1-dimethyl ethyl 3-amino-5-methyl isophthalic acid H-pyrazoles-1-manthanoate or 1, the preparation of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl is described below] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } benzoic preparation.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(fluorine first Base) ethyl] the oxygen base } phenylformic acid
With 3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } methyl benzoate (3.6g, 8.2mmol) mixture in THF (50mL) and water (50mL) is with 1M sodium hydroxide solution (8.2mL, 8.2mmol) handle, and in stirring at room 210 minutes.Remove THF in the vacuum, resistates is filtered, and extract with ethyl acetate (30mL).With water layer 2N hcl acidifying, with ethyl acetate (3 x 50mL) extraction, and with the organic extract liquid water (10mL) that merges, the washing of salt solution (10mL), and concentrate in a vacuum, obtained material requested, be light yellow foam (2.84g). 1H?NMR?δ(CDCl 3)：2.36-2.44(m，2H)，4.26(t，2H)，4.37(t，2H)，4.62-4.79(m，5H)，7.08(t，1H)，7.56-7.57(m，1H)，7.58-7.59(m，1H)，8.17(d，1H)，8.27(d，1H)；m/z?426(M+H) +，424(M-H) -
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } phenylformic acid is to adopt similar fashion, by 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } the methyl benzoate preparation.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl is described below] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } preparation of methyl benzoate.
3-{[5-(azetidine-1-base carbonyl)-3-chloropyridine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } methyl benzoate
Figure A200810169286D02101
With 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-5-methyl hydroxybenzoate (1.78g, 7.24mmol), salt of wormwood (2.0g, 14.5mmol) and 5-(azetidine-1-base carbonyl)-2, (2.08g, 8.7mmol) solution in acetonitrile (15mL) heated 1 hour in 130 ℃ in microwave reactor the 3-dichloropyridine.This solution is extracted water (3 x 50mL), salt solution (20mL) washing, dry (MgSO with ethyl acetate (150mL) 4), and remove in a vacuum and desolvate, obtained material requested, be orange (3.6g). 1H?NMR?δ(CDCl 3)：2.35-2.44(m，2H)，3.91(s，3H)，4.24-4.27(m，2H)，4.34-4.37(m，2H)，4.62-4.81(m，5H)，7.04(t，1H)，7.51(t，1H)，7.55(m，1H)，8.15(d，1H)，8.25(d，1H)；m/z?441(M+H) +
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base } methyl benzoate is to adopt similar method, by 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-preparation of 5-methyl hydroxybenzoate.
Figure A200810169286D02102
3-{[2-fluoro-1-(methyl fluoride) ethyl is described below] the oxygen base }-preparation of 5-methyl hydroxybenzoate.
3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-methyl hydroxybenzoate
Figure A200810169286D02103
10% palladium on carbon (Pd/C; 50mg) be added to 3-{[2-fluoro-1-(methyl fluoride) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (1.2g, 3.62mmol) and ammonium formiate (500mg, 8.0mmol) in the mixture in ethanol (7mL), then in microwave reactor in 140 ℃ of heating 20 minutes.This is reflected on the identical scale repeats, and two reaction mixtures are merged, filter, and, obtained material requested, be light yellow oil (2.1g) the filtrate vacuum concentration. 1H?NMR?δ(CDCl 3)：3.82(s，3H)，4.58-4.77(m，5H)，6.72(t，1H)，7.17-7.22(m，2H)；m/z?245(M-H) -
3-{[2-fluoro-1-(methyl fluoride) ethyl has been described in the front] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] preparation of methyl benzoate.
Embodiment 59:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D02111
1M hydrochloric acid (5mL) is added to 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide (90mg, 0.16mmol) in the solution in methyl alcohol (5mL), and with mixture in stirring at room 1 hour.Remove methyl alcohol in the vacuum, resistates is adjusted to pH6, be extracted into then in the ethyl acetate (3 x 50mL).The organism that merges is washed dry (MgSO with salt solution (50mL) 4), and vacuum concentration, obtained yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 0-5% methyl alcohol in ethyl acetate, has been obtained required compound, be white foam shape thing (28mg).
1H?NMR?δ(CDCl 3)：1.33(d，3H)，2.11(s，1H)，2.38(quin，2H)，2.55(s，3H)，3.74-3.82(m，2H)，4.26(t，2H)，4.55-4.62(m，1H)，4.69(t，2H)，6.97(t，1H)，7.30(t，1H)，7.40(t，1H)，8.13(s，1H)，8.35(d，1H)，8.41(s，1H)，8.85(d，1H)，9.53(d，1H)；m/z?465(M+H) +
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl is described below] the oxygen base }-5-[((1S)-2-{[(1, and the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-preparation of N-(5-methylpyrazine-2-yl) benzamide.
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D02121
With 3-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-(5-methylpyrazine-2-yl) benzamide (0.12g, 0.29mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (57mg, 0.29mmol) and salt of wormwood (80mg, 0.57mmol) mixture in acetonitrile (5mL) in microwave reactor in 140 ℃ the heating 1 hour.With this mixture vacuum concentration, and add ethyl acetate (50mL).With mixture water (50mL), salt solution (50mL) washing, dry (MgSO 4), filter and vacuum concentration, obtain brown oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 50-100% ethyl acetate in isohexane, has been obtained required compound, be colorless oil (90 mg). 1H NMR δ (CDCl 3) :-0.03 (s, 3H), 0.00 (s, 3H), 0.81 (s, 9H), 1.26 (d, 3H), 2.32 (quin, 2H), 2.49 (s, 3H), 3.61-3.76 (m, 2H), 4.20 (t, 2H), 4.46 (septet, 1H), 4.62 (t, 2H), 6.91 (t, 1H), 7.21 (t, 1H), 7.32 (t, 1H), 8.07 (s, 1H), 8.28 (d, 1H), 8.30 (s, 1H), 8.79 (d, 1H), 9.48 (d, 1H) .m/z 579 (M+H) +
3-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-5-hydroxy-n-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D02122
Lithium hydroxide monohydrate (0.38g, 9.12mol) mixture in water (20mL) is added to 3-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-5-[((1S)-2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide (1.94g, 3.65mol) in the solution in THF (40mL), and in stirring at room 20 hours.Remove THF in the vacuum, and water layer is adjusted to pH7.Add ethyl acetate (50mL) and water (50mL), then water layer is extracted in the ethyl acetate (50mL) again, and with the organic layer water (50mL), salt solution (50mL) washing that merge, dry (MgSO 4), filter and vacuum concentration.With roughage chromatography purification on silicon-dioxide, with the mixture wash-out of 20-50% ethyl acetate in isohexane, obtained material requested, be white solid (0.8g). 1HNMR?δ(CDCl 3)：0.04(s，3H)，0.08(s，3H)，0.87(s，9H)，1.30(d，3H)，2.56(s，3H)，3.65-3.81(m，2H)，4.46-4.51(m，1H)，5.63(s，1H)，6.63(t，1H)，6.98(t，1H)，7.04(t，1H)，8.15(s，1H)，8.41(s，1H)，9.57(s，1H)；m/z?418(M+H) +
3-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-5-[((1S)-and 2-{[(1, the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D02131
Tert-butyldimethylsilyl chloride (2.61g, 17.31mmol) be added to 3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl] the oxygen base-N-(5-methylpyrazine-2-yl) benzamide (1.5g, 4.95mmol) and imidazoles (2.36g, 34.62mmol) in the solution in DMF (15mL), and will react on stirring at room 24 hours.Add entry, and product is extracted in the ether (2x100mL).With the organism salt water washing that merges, dry (MgSO 4), filter and vacuum concentration, obtained yellow oil, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture wash-out of 0-40% ethyl acetate in isohexane, has been obtained material requested, be colorless oil (1.94g).
1H?NMR?δ(CDCl 3)：-0.03(s，3H)，0.01(s，3H)，0.16(s，6H)，0.81(s，9H)，0.92(s，9H)，1.23(d，3H)，2.48(s，3H)，3.58-3.74(m，2H)，4.37-4.42(m，1H)，6.54(t，1H)，6.86(t，1H)，6.99(t，1H)，8.06(s，1H)，8.21(s，1H)，9.48(d，1H).
The 3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D02132
Trimethyl silyl iodine (6.06mL, 42.75mmol) be added to 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base-N-(5-methylpyrazine-2-yl) benzamide (2.71g, 8.55mmol) in the solution in anhydrous acetonitrile (150mL), and stirred 24 hours.Add methyl alcohol (30mL) with stopped reaction, and stirred 10 minutes.10% w/v Sulfothiorine pentahydrate (20mL) is added in the mixture, and removes organic solvent in the vacuum.Resistates is adjusted to pH5 with 1M hydrochloric acid, and adds ethyl acetate (80mL).(1.4g) passes through filtering separation with yellow solid.Water layer is extracted in the ethyl acetate (2 x 80mL) again, and with the organic layer drying (MgSO that merges 4), remove in filtration and the vacuum and desolvate.This resistates and the yellow solid that obtains are above merged, and,, obtained this title compound (1.70g) with the mixture wash-out of 5%-10% methyl alcohol in DCM by the column chromatography purifying.
1H?NMR?δ(d 6-DMSO)：1.21(d，3H)，2.50(s，3H)，3.40-3.60(m，2H)，4.45(sex，1H)，4.80(t，1H)，6.50(s，1H)，6.97(s，1H)，7.08(s，1H)，8.32(s，1H)，9.21(s，2H)，9.63(s，1H)，10.80(brs，1H).m/z?304(M+H) +
3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl has been described in the front] the oxygen base }-preparation of N-(5-methylpyrazine-2-yl) benzamide.
Embodiment 59,3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide also is to adopt following method preparation.
With 3-hydroxyl-5-{[(1S)-2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide (3.03g, 10.0mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (2.17g, 11.0mmol) and salt of wormwood (4.14g, 30.0mmol) mixture in acetonitrile (30.3mL) is in 60 ℃ of heated overnight.Desolvate by evaporating to remove, and resistates is distributed between ethyl acetate (15vol) and water (10vol).Water further is extracted in the ethyl acetate, and with the organism water, the salt water washing that merge, dry (MgSO 4), remove in filtration and the vacuum and desolvate.With resistates SiO 2Chromatography purification with the mixture wash-out of 0.5-6% methyl alcohol in ethyl acetate, has obtained material requested, is foam (3.1g).The sample of described material (100mg) is dissolved in the acetonitrile of minimum volume, adds isohexane (5mL) then.Mixture was stirred 3 days, decant liquid, and resistates is developed with ether.Collect this crystalline material by filtering. 1H?NMR?δ(d 6-DMSO)：1.25(d，3H)，2.30(quin，2H)，2.48(s，3H)，3.49-3.61(m，2H)，4.09(t，2H)，4.55-4.63(m，3H)，4.92(t，1H)，7.12(t，1H)，7.47(t，1H)，7.57(t，1H)，8.37(d，1H)，8.58(d，1H)，8.69(d，1H)，9.26(d，1H)，11.06(s，1H)；m/z?465(M+H) +。100.6 ℃ of Mpt (fusing beginning).
Also can be by the slurries of this compound in ethyl acetate, toluene, Nitromethane 99Min. or methyl alcohol are stirred, crystallization goes out this title compound (125.0 ℃ of fusing points).
Embodiment 60:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-N-1H-pyrazole-3-yl benzamide
Figure A200810169286D02151
(2mL) is added to 1 trifluoroacetic acid, 1-dimethyl ethyl 3-({ [3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base) phenyl] carbonyl } amino)-1H-pyrazoles-1-manthanoate (115mg, 0.17mmol) in the solution in DCM (16mL), and in stirring at room 2 hours.Remove in the vacuum and desolvate, add DCM (20mL), and with mixture water (20mL), saturated sodium bicarbonate solution (20mL), salt solution (20mL) washing, dry (MgSO 4), filter and vacuum concentration, obtained required compound, be white foam shape thing (93mg).
1H?NMR?δ(CDCl 3)：1.36(d，3H)，2.37(quin，2H)，3.93-4.02(m，2H)，4.24(t，2H)，4.59-4.69(m，3H)，6.24(t，1H)，6.82(s，1H)，6.89(t，1H)，7.29-7.31(m，1H)，7.36-7.38(m，1H)，7.40(d，1H)，8.35(d，1H)，8.79(d，1H)，10.23(s，1H)，10.48(s，1H)；m/z?489(M+H) +
Describe below 1,1-dimethyl ethyl 3-({ [3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] oxygen base }-5-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base) phenyl] carbonyl } amino)-preparation of 1H-pyrazoles-1-manthanoate.
1,1-dimethyl ethyl 3-({ [3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base) phenyl] carbonyl } amino)-1H-pyrazoles-1-manthanoate
Figure A200810169286D02152
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.12mL, 0.92mmol) be added to 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base) phenylformic acid (0.26g, 0.61mmol) in the solution of DCM (8mL), and stirred 1 hour.Successively add 1,1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate (169mg, 0.92mmol) and pyridine (0.1mL, 1.23mmol), and restir 20 hours.With the reaction mixture vacuum concentration, and add ethyl acetate (50mL) and water (50mL).Water layer is extracted in the ethyl acetate (50mL) again, and with the organism water (50mL), salt solution (50mL) washing that merge, dry (MgSO 4), filter and vacuum concentration.Should thick oily matter chromatogram purification on silicon-dioxide, with the mixture wash-out of 25-50% ethyl acetate in isohexane, obtained required compound, be colorless oil (100mg).
1H?NMR?δ(CDCl 3)：1.38(d，3H)，1.62(s，9H)，2.38(quin，2H)，3.93-4.04(m，2H)，4.26(t，2H)，4.61-4.65(m，1H)，4.69(t，2H)，6.26(t，1H)，6.95(t，1H)，7.08(d，1H)，7.25-7.26(m，1H)，7.33(t，1H)，8.01(d，1H)，8.34(d，1H)，8.84(s，1H)，8.85(d，1H)；m/z?587(M-H) -
The front has described 1, the preparation of 1-dimethyl ethyl 3-amino-1H-pyrazoles-1-manthanoate.
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl is described below] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base) benzoic preparation.
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base) phenylformic acid
Lithium hydroxide monohydrate (48mg, 1.13mmol) solution in water (5mL) is added to 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base-5-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base) methyl benzoate (0.33g, 0.75mmol) in the solution in THF (10mL), and in stirring at room 20 hours.Remove THF in the vacuum, and water layer is washed to remove any impurity with ethyl acetate (50mL).With the water layer acidifying and be extracted in the ethyl acetate (2 x 50mL), use the salt water washing, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained required compound, be white foam shape thing (0.26g).m/z?424(M+H) +
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-([1S)-the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base) methyl benzoate
Figure A200810169286D02171
With 3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-5-methyl hydroxybenzoate (0.28g, 1.01mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (201mg, 1.01mmol) and salt of wormwood (280mg, 2.03mmol) mixture in acetonitrile (5mL) stirred 5 hours in 140 ℃ in microwave reactor.With the reaction mixture vacuum concentration, add ethyl acetate (50mL), and with organism water (50mL), salt solution (50mL) washing, dry (MgSO 4), filter and vacuum concentration.Should thick oily matter chromatogram purification on silicon-dioxide, with the mixture wash-out of 20-70% ethyl acetate in isohexane, having obtained required compound is colorless oil (330mg). 1H?NMR?δ(CDCl 3)：1.38(d，3H)，2.37(quin，2H)，3.90(s，3H)，3.93-4.04(m，2H)，4.26(d，2H)，4.61-4.70(m，3H)，6.25(t，1H)，6.95(t，1H)，7.43-7.44(m，1H)，7.49-7.50(m，1H)，8.32(d，1H)，8.85(d，1H)；m/z438(M+H) +
3-((1S)-and the 2-[(difluoromethyl) the oxygen base]-the 1-methylethyl } the oxygen base)-the 5-methyl hydroxybenzoate
Figure A200810169286D02172
3-({ (1S)-2-[(difluoromethyl) oxygen base]-the 1-methylethyl } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] methyl benzoate (0.48g, 1.08mmol) be dissolved among ethanol (10mL) and the THF (10mL), flask is vacuumized, and purge with argon gas (3 times).Add 10% palladium on carbon (140mg), flask is vacuumized again, and use hydrogen purge at last.With reaction mixture in stirring at room 20 hours till reaction is finished.Reaction mixture is vacuumized, and, pass through then with argon purge (3 times)
Figure A200810169286D0217184545QIETU
Remove by filter catalyzer.With the filtrate vacuum concentration, obtained required compound, be colorless oil (1.05g). 1H?NMR?δ(CDCl 3)：1.35(d，3H)，3.90(s，3H)，3.90-4.02(m，2H)，4.57-4.64(m，1H)，5.20(s，1H)，6.26(t，1H)，6.63(t，1H)，7.14-7.15(m，1H)，7.17-7.18(m，1H)；m/z?275(M-H) -
3-({ (1S)-2-[(difluoromethyl) oxygen base has been described in the front]-the 1-methylethyl } the oxygen base)-the 5-[(phenyl methyl) the oxygen base] preparation of methyl benzoate and 2-(azetidine-1-base carbonyl)-5-chloropyrazine.
Embodiment 61:3-{[5-(azetidine-1-base alkylsulfonyl)-4-methyl isophthalic acid, the 3-thiazol-2-yl] oxygen Base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzoyl Amine
Figure A200810169286D02181
Cesium carbonate (293mg; 0.9mmol) be added to 3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl] the oxygen base-N-(5-methylpyrazine-2-yl) benzamide (95mg; 0.3mmol) and 5-(azetidine-1-base alkylsulfonyl)-2-chloro-4-methyl isophthalic acid; 3-thiazole (90mg; 0.36mmol) in the solution in acetonitrile (5mL), and the mixture that will stir in microwave reactor in 120 ℃ of heating 1 hour.Mixture is cooled to room temperature and normal pressure, removes acetonitrile in the vacuum, and resistates is distributed between water (15mL) and ethyl acetate (30mL).Organic layer salt water washing, dry (MgSO 4) and vacuum-evaporation to resistates, it at the enterprising circumstances in which people get things ready for a trip of silicon-dioxide spectrum purifying, with the mixture wash-out of 60% ethyl acetate in isohexane, has been obtained material requested, be colorless solid (79mg).
1H?NMR?δ(CDCl 3)：1.3(d，3H)，2.15(m，2H)，2.5(s，3H)，2.5(s，3H)，3.35(s，3H)，3.45-3.55(m，2H)，3.85(t，4H)，4.55(m，1H)，7.0(d，1H)，7.35(d，2H)，8.05(s，1H)，8.3(s，1H)，9.45(s，1H)；m/z?534(M+H) +
3-hydroxyl-5-{[(1S)-1-methyl-2-(methoxyl group) ethyl has been described in the front] the oxygen base }-preparation of N-(5-methylpyrazine-2-yl) benzamide.
5-(azetidine-1-base alkylsulfonyl)-2-chloro-4-methyl isophthalic acid is described below, the preparation of 3-thiazole.
5-(azetidine-1-base alkylsulfonyl)-2-chloro-4-methyl isophthalic acid, the 3-thiazole
Figure A200810169286D02182
2-chloro-4-methylthiazol-5-alkylsulfonyl chlorine (462mg; 2.0mmol) solution in DCM (5mL) is added to ice-cold azetidine hydrochloride (196mg; 2.1mmol) and triethylamine (1.0mL; 7.2mmol) in the solution in DCM (15mL), and mixture stirred 30 minutes in 0 ℃.Remove DCM in the vacuum, obtained resistates, it is distributed between water (50mL) and ethyl acetate (75mL).Organic layer salt water washing, dry (MgSO 4) and evaporation, with resistates chromatogram purification on silicon-dioxide,, obtained material requested with the mixture wash-out of 10% ethyl acetate in isohexane, be crystalline solid (100mg) from ethyl acetate and isohexane.
1H?NMR?δ(CDCl 3)：2.2(dt，2H)，2.65(s，3H)，3.9(t，4H)；m/z253(M+H) +
Embodiment 62:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-[1-(difluoromethyl)-1H-pyrazole-3-yl]-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] benzamide
Figure A200810169286D02191
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (0.088mL, 0.66mmol) be added to 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid (171mg, 0.44mmol) in the solution in DCM (10mL), and will react on stirring at room 30 minutes.Add successively then 1-(difluoromethyl) pyrazoles-3-amine hydrochlorate (75mg, 0.44mmol) and DIPEA (0.154mL, 0.89mmol).Gained solution in stirring at room 16 hours, is removed in the vacuum then and desolvates, and add ethyl acetate (20mL) and water (20mL).Water with ethyl acetate (2 x 20mL) extraction, is washed with saturated sodium bicarbonate solution (20mL) and salt solution (20mL).With the organic extract liquid drying (MgSO that merges 4), filter and evaporation.Resistates is carried out the flash column chromatography purifying on silicon-dioxide,, obtain required product, be white solid (132mg) with the mixture wash-out of 50-100% ethyl acetate in isohexane. 1H?NMR?δ(CDCl 3)：2.40-2.12(m，4H)，4.03-3.89(m，4H)，4.25(t，2H)，4.71(t，2H)，5.01-4.96(m，1H)，6.76(t，1H)，7.03(t，1H)，7.08(d，1H)，7.11(s，1H)，7.22(s，1H)，7.38(dd，1H)，7.75(d，1H)，8.13(d，1H)，8.33(d，1H)，8.50(s，1H)； 19F?NMR?δ(CDCl 3)：-93.84；m/z?500(M+H) +.
Following compound is to adopt similar method preparation.
Figure A200810169286D02201
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl has been described in the front] the oxygen base }-5-[(3S)-and tetrahydrofuran (THF)-3-base oxygen base] phenylformic acid, 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid and 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1S)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzoic preparation.
The preparation of 1-(difluoromethyl) pyrazoles-3-amine has been described in the document [WO2005090332, PCT Int.Appl. (2005)].
Embodiment 63:3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-first pyrazine-2-yl) benzamide
Figure A200810169286D02202
At following 1-chloro-N of argon gas, N, 2-trimethylammonium-1-propenyl amine (0.042mL, 0.31mmol) be added to 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid (0.102g, 0.0.31mmol) in the solution in DCM (3mL), and in stirring at room 40 minutes.Add 2-amino-5-methylpyrazine (57mg, 0.52mmol) and pyridine (0.043mL 0.52mmol) also will react restir 3 hours.Remove in the vacuum and desolvate, and resistates is dissolved in the ethyl acetate (30mL), water (2 x 10mL), saturated sodium bicarbonate aqueous solution (10mL), salt solution (10mL) washing, dry (MgSO 4), filter, and vacuum-evaporation.With roughage chromatography purification on silicon-dioxide, with the mixture wash-out of 60-100% ethyl acetate in isohexane, obtained required compound, be white foam shape thing (86mg).
1H?NMR?δ(CDCl 3)：1.35(d，3H)，2.32-2.44(m，2H)，2.56(s，3H)，3.41(s，3H)，3.47-3.64(m，2H)，4.26(t，2H)，4.62(q，1H)，4.69(t，2H)，6.98(s，1H)，7.29(s，1H)，7.41(s，1H)，8.13(s，1H)，8.35(s，2H)，8.86(s，1H)，9.54(s，1H)；m/z?479(M+H) +
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl is described below] the oxygen base }-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } benzoic preparation.
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } phenylformic acid
Figure A200810169286D02211
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base } methyl benzoate (1.06g, 2.6mmol) be dissolved in THF (24mL) and the methyl alcohol (8mL), add 1N lithium hydroxide solution (3.12mL) then, drip water (10mL) then.Gained solution adds another part 1N lithium hydroxide solution (1.3mL) in stirring at room 3 hours, this reaction restir 2 hours, and then add 1N lithium hydroxide solution (0.6mL), and continue again to stir 1 hour.Remove organism by reduction vaporization, and the aqueous solution is washed with ether (10mL), use the 2N hcl acidifying then, and extract with ethyl acetate (3 x 20mL).The organism water (10mL), salt solution (10mL) washing and the reduction vaporization that merge are extremely done, and having obtained required compound is white solid (887mg).
1H?NMR?δ(CDCl 3)：1.34(d，3H)，2.38(quin，2H)，3.41(s，3H)，3.47-3.64(m，2H)，4.27(t，2H)，4.55-4.65(m，1H)，4.69(t，2H)，6.99(s，1H)，7.45(s，1H)，7.55(s，1H)，8.33(s，1H)，8.84(s，1H)；m/z388(M+H) +
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1R)-1-methyl-2-(first The oxygen base) ethyl] the oxygen base } methyl benzoate
Figure A200810169286D02221
With 3-hydroxyl-5-{[(1R)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } methyl benzoate (727mg, 3mmol), 2-(azetidine-1-base carbonyl)-5-chloropyrazine (712mg, 3.6mmol) and salt of wormwood (828mg, 6mmol) mixture in DMA (10mL) stirred 2 hours at 120 ℃.This solution is diluted water (3 x 50mL), salt solution (20mL) washing, dry (MgSO with ethyl acetate (150mL) 4), remove in filtration and the vacuum and desolvate.Resistates by chromatography purification on silicon-dioxide, with the mixture wash-out of 50% ethyl acetate in isohexane, has been obtained material requested, be colorless oil (1.07g). 1H NMR δ (CDCl 3): 1.27 (d, 3H), 2.4 (quintet, 2H), 3.4 (s, 3H), 3.5-3.6 (m, 2H), 3.9 (s, 3H), 4.25 (t, 2H), 4.53 (q, 1H), 4.7 (t, 2H), 6.95 (s, 1H), 7.4 (s, 1H), 7.5 (s, 1H), 8.3 (s, 1H), 8.85 (s, 1H); M/z402 (M+H) +
The preparation of 2-(azetidine-1-base carbonyl)-5-chloropyrazine has been described in the front.
The 3-hydroxyl-5-{[(1R)-1-methyl-2-(methoxyl group) ethyl] the oxygen base } methyl benzoate
Figure A200810169286D02222
With 3-{[(1R)-1-methyl-2-(methoxyl group) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] solution that contains 10% palladium on carbon of methyl benzoate in THF (5mL) and ethanol (5mL) stirred 16 hours under hydrogen.Remove by filter palladium on carbon, and, obtained material requested, be copal gum shape thing (723mg) filtrate evaporated under reduced pressure.
1H?NMR?δ(CDCl 3)：1.31(d，3H)，3.42(s，3H)，3.48-3.64(m，2H)，3.88(s，3H)，4.54-4.63(m，1H)，5.75(s，1H)，6.64(s，1H)，7.11(s，1H)，7.17(s，1H)；m/z?241(M+H) +
3-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] methyl benzoate
Figure A200810169286D02231
Under argon gas in 0 ℃ DIAD (4.6g, 0.029mol) be added drop-wise to 3-hydroxyl-5-{[phenyl methyl] the oxygen base methyl benzoate (6g, 0.023mol), (S)-(+)-1-methoxyl group-2-propyl alcohol (2.59g, 0.029mol) and triphenylphosphine (7.53g is 0.029mol) in the solution in THF (100mL).To react on 0 ℃ and stir 1 hour, and in stirring at room 20 hours.Remove the volatility thing in the vacuum, and add isohexane/ethyl acetate 2:1, stirred then 1 hour.Remove by filter white solid, and filtrate is evaporated to resistates, it at the enterprising circumstances in which people get things ready for a trip spectrum of silicon-dioxide purifying, with the mixture gradient elution of 0-20% ethyl acetate in isohexane, has been obtained required compound (5.11g).
1H?NMR?δ(CDCl 3)：1.31(d，3H)，3.40(s，3H)，3.45-3.60(m，2H)，3.88(s，3H)，4.57(sex，1H)，5.07(s，2H)，6.76(m，1H)，7.25(m，2H)，7.40(m，5H).m/z?331(M+H) +.
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate.
Embodiment 64:3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-{[(1R)-and 1-methyl-2-(methoxyl group) ethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide
Figure A200810169286D02232
With 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-hydroxy-n-(5-methylpyrazine-2-yl) benzamide (101mg, 0.25mmol), S-(+)-methoxyl group-2-propyl alcohol (0.049mL, 0.50mmol) and triphenylphosphine (131mg, 0.50mmol) mixture in anhydrous THF (10mL) under argon gas in 0 ℃ by (0.099mL 0.50mmol) handles with dropwise using DIAD.Mixture heating up to room temperature and stirring spent the night.Remove THF by reduction vaporization, and resistates is passed through chromatogram purification twice on silicon-dioxide, with the mixture wash-out of 0-7% methyl alcohol in DCM.Required fraction is merged, and carry out purifying, use the mixture wash-out of 50% 7N ammonia (in methyl alcohol) in DCM then with the mixture of 5% methyl alcohol in DCM by SCX post wash-out.Required fraction is passed through NH 2The post wash-out carries out purifying, with the mixture wash-out of 5% methyl alcohol in DCM, has obtained required compound (35mg), estimates that purity is 85%. 1H?NMR?δ(CDCl 3)：1.26(d，3H)，2.28(quin，2H)，2.49(s，3H)，3.33(s，3H)，3.41-3.54(m，2H)，4.18(t，2H)，4.50-4.58(m，1H)，4.64(t，2H)，6.77(s，1H)，7.08(s，1H)，7.25(s，1H)，7.31(d，1H)，8.04(d，1H)，8.07(s，1H)，8.26(d，1H)，8.30(s，1H)，9.46(s，1H)；m/z?478(M+H) +
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl is described below] the oxygen base }-preparation of 5-hydroxy-n-(5-methylpyrazine-2-yl) benzamide.
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-5-hydroxy-n-(5-methyl pyrrole Piperazine-2-yl) benzamide
Figure A200810169286D02241
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(phenyl methyl) the oxygen base] benzamide (3.9g, 7.9mmol) be dissolved in ethyl acetate (200mL) and the ethanol (200mL), add 10% palladium on carbon then.To be reflected under the hydrogen and stir 16 hours.Add methyl alcohol (150mL) promoting the dissolving of material, and, resistates is dissolved among the DMF, filter, and the filtrate that merges is evaporated to dried, obtained material requested (3.17g) suspension filtered. 1H?NMR?δ(d 6-DMSO)：2.33(quin，2H)，2.53(s，3H)，4.13(t，2H)，4.64(t，2H)，6.81(s，1H)，7.31(s，1H)，7.34(s，1H)，7.62(d，1H)，8.05(d，1H)，8.40(s，1H)，8.47(s，1H)，9.26(s，1H)，10.35(s，1H)，11.02(s，1H)；m/z406(M+H) +
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-N-(5-methylpyrazine-2-yl)-5-[(phenyl methyl) the oxygen base] benzamide
Figure A200810169286D02242
1-chloro-N, N, 2-trimethylammonium-1-propenyl amine (2.67mL, 20mmol) be added to 3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid (6.18g, 15.3mmol) in the solution in DCM (100mL), and will react on stirring at room 50 minutes.Add 2-amino-5-methylpyrazine (3.34g, 30.6mmol) and pyridine (2.5mL 30.6mmol), and will react stirring and spend the night.Remove in the vacuum and desolvate, and resistates is dissolved in the ethyl acetate (350mL).Organic phase water (2 x 100mL), salt solution (100mL) washing, dry (MgSO 4), filter, and vacuum-evaporation.With resistates SiO 2Chromatography purification with the mixture wash-out of 50-75% ethyl acetate in isohexane, has obtained material requested (4.01g). 1H NMR δ (CDCl 3): 2.28 (quin, 2H), 2.49 (s, 3H), 4.18 (t, 2H), 4.63 (t, 2H), 5.05 (s, 2H), 6.78 (s, 1H), 7.10 (s, 1H), 7.25-7.37 (m, 7H), 8.04 (d, 1H), 8.07 (s, 1H), 8.25 (s, 2H), 9.46 (s, 1H); M/z 496 (M+H) +
3-{[6-(azetidine-1-base carbonyl) pyridin-3-yl] the oxygen base }-the 5-[(phenyl methyl) the oxygen base] phenylformic acid
With 3-hydroxyl-5-{[phenyl methyl] the oxygen base } methyl benzoate (5.16g, 20mmol), 2-(azetidine-1-base carbonyl)-5-bromopyridine (5.3g, 22mmol), cesium carbonate (19.56g, 60mmol) (3.73g, 4mmol) mixture in DMA (100mL) stirred 6 hours in 160 ℃ in microwave reactor with bromine three (triphenylphosphine) copper.With the DMA reduction vaporization, resistates is dissolved in the water (200mL), and with and ethyl acetate (3 x 50mL) washing.With water layer 2N hcl acidifying, and with ethyl acetate (3 x 100mL) extraction, with organism water (2 x 20mL), the salt solution (20mL) that merges, dry (MgSO 4), remove in filtration and the vacuum and desolvate, obtained material requested (6.18g). 1H?NMR?δ(CDCl 3)：2.29(s，2H)，4.20(s，2H)，4.64(s，2H)，5.04(s，2H)，6.83(s，1H)，7.22-7.44(m，7H)，7.49(s，1H)，7.79-8.63(m，2H)；m/z?405(M+H) +
3-hydroxyl-5-{[phenyl methyl has been described in the front] the oxygen base } preparation of methyl benzoate and 2-(azetidine-1-base carbonyl)-5-bromopyridine.
Biology
Experiment:
Can adopt following method, the biological activity of all cpds of mensuration formula (I):
(1) enzymic activity
Measure the enzymic activity of recombinant human pancreas GLK by cultivating GLK, ATP and glucose.By measuring the coupling with G-6-P desaturase, NADP/NADPH system, and measure 340nm optical density(OD) down linearity in time and increase, determine speed people such as (, 1993) Matschinsky of product formation.Adopt the method that people such as Brocklehurst describes (Diabetes 2004,53,535-541), GLKRP exist or non-existent condition under, can assessing compound to the activation of GLK.
The formation of reorganization GLK and GLKRP:
Use Sambrook, Fritsch ﹠amp; Maniatis T, the prior art of describing in 1989 by people's pancreas and people liver mRNA, obtains people GLK and GLKRP cDNA through PCR respectively.According to people such as Tanizawa (1991) and Bonthron, people such as D.T (later corrected in Warner, J.P.1995) GLK shown in and GLKRP cDNA sequence, design PCR introduction.
On Bluescript II carrier, clone
Use pBluescript II people 1998 such as () Short, go up clone GLK and GLKRP cDNA intestinal bacteria (E.coli), wherein be the recombinant cloning vector system, with used similar of people (1985) such as Yanisch-Perron C, the colEI-base replicon that comprises band polylinker dna segment, described polylinker dna segment contains the restriction site of a plurality of uniquenesses, is attacked from the side by phage T3 and T7 promoter sequence (marker gene of a kind of filobactivirus of duplicating and a kind of medicine of anti-the penbritin).
Transform
Usually carry out colibacillary conversion by electroporation.Making 400ml DH5a or BL21 (DE3) strain nutrient solution grow to OD 600 in L-meat soup is 0.5, and passes through 2 centrifugal results under the 000g.With cell washed twice in ice-cooled deionized water, be suspended in 1ml 10% glycerine storage of under-70 ℃, merotomizing once more.Use Millipore V series TMFilm (0.0025mm aperture) will connect mixture (Ligation mixes) desalination.In 0.2cm electroporation cuvette, the 40ml cell is connected mixture or plasmid DNA places ice to cultivate 10 minutes with 1ml, use Gene Pulser subsequently TMInstrument (BioRad) is at 0.5kVcm -1, carry out pulse under the 250mF.In the L-agar that is supplemented with 10mg/ml tsiklomitsin or 100mg/ml penbritin, select to transform.
Express
Express GLK from the pTB375NBSE carrier of e. coli bl21 cell, obtain containing the recombinant protein of the 6-His mark that is in close proximity to the terminal methionine(Met) of N-.Perhaps, another kind of suitable carrier is pET21 (+) DNA (Novagen, a Cat numbering 697703).Use the 6-His mark, thereby can on the post that is filled with nickel-complexon I agarose (available from Qiagen, Cat numbering 30250), carry out purifying recombinant protein.
Express GLKRP from pFLAG CTC (IBI Kodak) carrier of e. coli bl21 cell, obtain containing the recombinant protein of the terminal FLAG mark of C-.Described albumen at first through DEAE agarose ion-exchange purification, is then utilized the FLAG mark, resist at M2-the FLAG immune affinity column on (numbering A1205) and carry out whole purifying available from Sigma-Aldrich.
(2) oral glucose tolerance test (OGTT)
Conscious Zucker obese fa/fa mouse (12-13 age or bigger in week) is carried out oral glucose tolerance test, and rat has at least before test to be raised with high fat diet (45%kcal fat) in 2 weeks.Animal fasting 2 hours before test.Per os waits to try compound or carrier, and per os gives the glucose solution of 2g/kg body weight after 120 minutes.Before or after giving glucose,,, adopt the Accucheck glucometer to measure glucose level through the tail blood sampling in different time points (60 minutes at interval).Form glucose level-time curve, and calculate area (AUC) under 120 minutes the curved surface when being designated as zero (give glucose).AUC with the vehicle Control group is a null value, measures percent inhibition.
The compounds of this invention generally has the activity of activation, its EC to glucokinase 50Be lower than about 1 μ M, and generally be lower than about 500nM.For example, embodiment 3 has the EC of 31nm 50
Embodiment 3 shows 54% OGTT activity at 10mg/kg.
Reference
1 Printz，R.L.，Magnuson，M.A.and?Granner，D.K.(1993)Annual?Review?of Nutrition?13，463-96
2 DeFronzo，R.A.(1988)Diabetes?37，667-87
3 Froguel，P.，Zouali，H.，Vionnet，N.，Velho，G.，Vaxillaire，M.，Sun，F.，Lesage，S.， Stoffel，M.，Takeda，J.and?Passa，P.(1993)New?England?Journal?of?Medicine?328， 697-702
4 Bell，G.I.，Pilkis，S.J.，Weber，I.T.and?Polonsky，K.S.(1996)Annual?Review?of Physiology?58，171-86
5 Velho，G.，Petersen，K.F.，Perseghin，G.，Hwang，J.H.，Rothman，D.L.，Pueyo，M. E.，Cline，G.W.，Froguel，P.and?Shulman，G.I.(1996)Journal?of?Clinical Investigation?98，1755-61
6 Christesen，H.B.，Jacobsen，B.B.，Odili，S.，Buettger，C.，Cuesta-Munoz，A.， Hansen，T.，Brusgaard，K.，Massa，O.，Magnuson，M.A.，Shiota，C.，Matschinsky，F. M.and?Barbetti，F.(2002)Diabetes?51，1240-6
6a Gloyn，A.L.，Noordam，K.，Willemsen，M.A.A.P.，Ellard，S.，Lam，W.W.K.， Campbell，I.W.，Midgley，P.，Shiota，C.，Buettger，C.，Magnuson，M.A.， Matschinsky，F.M.，and?Hattersley，A.T.；Diabetes?52：2433-2440
7 Glaser，B.，Kesavan，P.，Heyman，M.，Davis，E.，Cuesta，A.，Buchs，A.，Stanley，C. A.，Thornton，P.S.，Permutt，M.A.，Matschinsky，F.M.and?Herold，K.C.(1998) New?England?Journal?of?Medicine?338，226-30
8 Caro，J.F.，Triester，S.，Patel，V.K.，Tapscott，E.B.，Frazier，N.L.and?Dohm，G.L. (1995)Hormone?&?Metabolic?Research?27，19-22
9 Desai，U.J.，Slosberg，E.D.，Boettcher，B.R.，Caplan，S.L.，Fanelli，B.，Stephan，Z.， Gunther，V.J.，Kaleko，M.and?Connelly，S.(2001)Diabetes?50，2287-95
10 Shiota，M.，Postic，C.，Fujimoto，Y.，Jetton，T.L.，Dixon，K.，Pan，D.，Grimsby，J.， Grippo，J.F.，Magnuson，M.A.and?Cherrington，A.D.(2001)Diabetes?50，622-9
11 Ferre，T.，Pujol，A.，Riu，E.，Bosch，F.and?Valera，A.(1996)Proceedings?of?the National?Academy?of?Sciences?of?the?United?States?of?America?93，7225-30
12 Seoane，J.，Barbera，A.，Telemaque-Potts，S.，Newgard，C.B.and?Guinovart，J.J. (1999)Journal?of?Biological?Chemistry?274，31833-8
13 Moore，M.C.，Davis，S.N.，Mann，S.L.and?Cherrington，A.D.(2001)Diabetes Care?24，1882-7
14 Alvarez，E.，Roncero，I.，Chowen，J.A.，Vazquez，P.and?Blazquez，E.(2002)Journal of?Neurochemistry?80，45-53
15 Lynch，R.M.，Tompkins，L.S.，Brooks，H.L.，Dunn-Meynell，A.A.and?Levin，B.E. (2000)Diabetes?49，693-700
16 Roncero，I.，Alvarez，E.，Vazquez，P.and?Blazquez，E.(2000)Journal?of Neurochemistry?74，1848-57
17 Yang，X.J.，Kow，L.M.，Funabashi，T.and?Mobbs，C.V.(1999)Diabetes?48，1763- 1772
18 Schuit，F.C.，Huypens，P.，Heimberg，H.and?Pipeleers，D.G.(2001)Diabetes?50，1- 11
19 Levin，B.E.(2001)International?Journal?of?Obesity?25，supplement?5，S68-S72.
20 Alvarez，E.，Roncero，I.，Chowen，J.A.，Thorens，B.and?Blazquez，E.(1996)Journal of?Neurochemistry?66，920-7
21 Mobbs，C.V.，Kow，L.M.and?Yang，X.J.(2001)American?Journal?of?Physiology- Endocrinology?&?Metabolism?281，E649-54
22 Levin，B.E.，Dunn-Meynell，A.A.and?Routh，V.H.(1999)American?Journal?of Physiology?276，R1223-31
23 Spanswick，D.，Smith，M.A.，Groppi，V.E.，Logan，S.D.and?Ashford，M.L.(1997) Nature?390，521-5
24 Spanswick，D.，Smith，M.A.，Mirshamsi，S.，Routh，V，H，and?Ashford，M.L.(2000) Nature?Neuroscience?3，757-8
25 Levin，B.E.and?Dunn-Meynell，A.A.(1997)Brain?Research?776，146-53
26 Levin，B.E.，Govek，E.K.and?Dunn-Meynell，A.A.(1998)Brain?Research?808， 317-9
27 Levin，B.E.，Brown，K.L.and?Dunn-Meynell，A.A.(1996)Brain?Research?739， 293-300
28 Rowe，I.C.，Boden，P.R.and?Ashford，M.L.(1996)Journal?of?Physiology?497，365- 77
29 Fujimoto，K.，Sakata，T.，Arase，K.，Kurata，K.，Okabe，Y.and?Shiraishi，T.(1985) Life?Sciences?37，2475-82
30 Kurata，K.，Fujimoto，K.and?Sakata，T.(1989)Metabolism：Clinical?&?Experimental 38，46-51
31 Kurata，K.，Fujimoto，K.，Sakata，T.，Etou，H.and?Fukagawa，K.(1986)Physiology &?Behavior?37，615-20
32 Jetton?T.L.，Liang?Y.，Pettepher?C.C.，Zimmerman?E.C.，Cox?F.G.，Horvath?K.， Matschinsky?F.M.，and?Magnuson?M.A.，J.Biol.?Chem.，Feb?1994；269：3641-3654
33 Reimann?F.and?Gribble?F.M.，Diabetes?2002?51：2757-2763
34 Cheung?A.T.，Dayanandan?B.，Lewis?J.T.，Korbutt?G.S.，Rajotte?R.V.，Bryer- Ash?M.，Boylan?M.O.，Wolfe?M.M.，Kieffer?T.J.，Science，Vol?290，Issuo?5498， 1959-1962，8?December?2000

Claims (8)

1. compound
3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-{[(1S)-and 2-hydroxyl-1-methylethyl] the oxygen base }-N-(5-methylpyrazine-2-yl) benzamide;
Figure A200810169286C00021
Or its pharmacologically acceptable salt, solvate or prodrug.
2. comprise compound or pharmaceutically acceptable salt thereof, solvate or the prodrug of claim 1 and the pharmaceutical composition of pharmaceutically acceptable diluent or carrier.
3. be used as compound or pharmaceutically acceptable salt thereof, solvate or the prodrug of the claim 1 of medicine.
4. the compound of claim 3, wherein said medicine are to be used for the treatment of by the disease of the GLK mediation especially medicine of type ii diabetes.
5. the compound or pharmaceutically acceptable salt thereof of claim 1, solvate or prodrug are used for the treatment of application aspect the medicine of the disease of GLK mediation in preparation.
6. the compound or pharmaceutically acceptable salt thereof of claim 1, solvate or prodrug are used for the treatment of the application aspect the medicine of type ii diabetes in preparation.
7. the method for preparing the described compound of claim 1, described method comprises makes 3-{[5-(azetidine-1-base carbonyl) pyrazine-2-yl] the oxygen base }-5-[((1S)-2{[(1, and the 1-dimethyl ethyl) (dimethyl) silyl] the oxygen base }-the 1-methylethyl) the oxygen base]-N-(5-methylpyrazine-2-yl) benzamide and hydrochloric acid reacts in methyl alcohol; And then, if desired: i) remove any protecting group; Ii) end product is converted into its pharmacy acceptable salt, solvate or prodrug.
8. the compound of claim 1 or other material of its pharmacy acceptable salt, solvate or prodrug and one or more and/or methods of treatment combine.
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