CN101381657B - Method for cleaning floor - Google Patents

Abstract

The present invention relates to a stable cleaning composition including a borate salt and spores (bacterial or fungal), vegetative bacteria, or fungi. The composition can also include a polyol.

Description

A kind of method on cleaning ground

The application is the divisional application of Application No. 200480031302X applications.

Technical field

The present invention relates to stable Cleasing compositions, it includes borate and spore (bacterium or fungi), nutrition (vegetative) bacterium or fungi, and uses the method for said composition.Said composition also includes polyalcohol.

Background technology

Spore, bacterium and fungi play an important role in Cleasing compositions in those Cleasing compositions especially used in cleaning drainpipe and grease trap.Existing composition containing spore, bacterium or fungi is typically provided with " two parts " product form, and one of container is biological components and second container is chemical cleaner.Mixed chemical cleaning agent and biological components, it is impossible then to store the mixture, because chemicals has negative effect to spore, bacterium or fungi.Still need the Cleasing compositions (for example, " single part " composition) while including chemical cleaner and spore, bacterium or the stabilization of fungi.

Summary of the invention

The present invention relates to stable Cleasing compositions, it includes borate and spore (bacterium or fungi), bacterial nutrition or fungi, and uses the method for said composition.Said composition also includes polyalcohol.

In one embodiment, composition of the invention includes spore, bacterium or the fungi of borate and effective cleaning amount.Borate may include alkanolamine borate.Borate and/or composition can be substantially free of sodium ions.In one embodiment, composition of the invention can be provided containing spore (bacterium or fungi), bacterial nutrition or the preparation of fungi, and said preparation has suitable stability under the pH more than or equal to 9.In one embodiment, composition of the invention can be provided containing spore (bacterium or fungi), bacterial nutrition or the preparation of fungi, and said preparation has suitable stability under water most about 65wt%'s.

The Cleasing compositions of the present invention may also comprise the one or more in non-ionic surfactant, silicone surfactant, anion surfactant and hydrotropic agent.The Cleasing compositions may include the nonionic surfactant of one or more about 0.003 to about 35wt%, about 0.0005 to about 35wt% silicone surfactant, about 0.003 to about 35wt% anion surfactant, and about 0.001 to about 20wt% hydrotropic agent.The Cleasing compositions may include nonionic surfactant and silicone surfactant.The Cleasing compositions may include about 0.5 to about 35wt% nonionic surfactant and about 0.1 to about 35wt% silicone surfactant.

The method of the present invention may include to apply composition of the invention to surface to be cleaned or object.The composition applied can be stable microbial composite or Cleasing compositions.Surface or object to be cleaned may include the one or more in ground, drainpipe or floor drain.In one embodiment, the method for the present invention may include to increase the coefficient of friction on surface.In one embodiment, the present invention may include cleaning mortar (grout).In one embodiment, surface or mortar are ground (floor) or earth material (flooring).

Brief description

Fig. 1, which is shown, carry out the result obtained by coefficient of friction (skid resistance) measurement for the ceramic tile in restaurant kitchen weekly.

Fig. 2A and 2B show that the present composition cleans the mortar on the clinker tile ground in kitchen at the restaurant.Fig. 2A illustrates the ground before the present composition is applied.Fig. 2 B illustrate the ground after the present invention is applied.

Fig. 3 shows a part of ground cleaned using a part of ground (left side) of conventional Cleasing compositions cleaning and with the composition of the present invention.

Detailed description of the invention

Definition

" microorganism formulation " as used herein refers to that it can be provided in preservative containing one or more compositions in spore (bacterium or fungi), bacterial nutrition or fungi." bacteria preparation " as used herein refers to the composition containing bacterial spore and/or bacterial nutrition, and it can be provided in preservative.Preservative may include any or various antiseptic composition for example used in the spore (bacterium or fungi), bacterial nutrition or the preparation of fungi of commercial offers.This preservative may include such as chelating agent, surfactant, buffer, water or the like.Microorganism formulation can for example digest or dirt of degrading, for example fat, oil, lubricating grease, sugar, protein, carbohydrate or the like.

The representations such as percetage by weight (wt%) as used herein, weight %, % weight are synonymous, and they refer to when the weight of the material divided by the weight of composition and material concentration when being multiplied by 100.

The salt and borate of boric acid as used herein are used interchangeably, and they refer to the salt of such as potassium borate, monoethanolamine borate etc, or can obtain or imaginabale another salt, such as by neutralizing another salt that boric acid is obtained.The salt of boric acid or the percetage by weight of borate can be expressed as the ion containing electronegative boron in the compositions of the present invention, the percetage by weight of such as borate and/or boric acid moieties, or whole borates, such as the percetage by weight of both parts of electronegative part and positively charged.Preferably, percetage by weight refers to the salt of whole boric acid.The percetage by weight of the salt of citric acid or other sour salt can also these modes express, preferably refer to the salt of all acid.Term used herein " whole boron compounds " refers to borate and boric acid moieties sum.

Alkali or alkaline pH as used herein refer to that pH is more than 7, more than or equal to 8, about 8 to about 9.5, about 8 to about 11, greater than about 9, or about 9 to about 10.5.

" substantially free of sodium ion " as used herein refers to the composition containing less than about 1wt% sodium ions.The embodiment of the present composition may include the sodium ion less than 1wt%, sodium ion less than 0.75wt%, sodium ion less than 0.5wt%, sodium ion less than 0.25wt%, sodium ion less than 0.2wt%, sodium ion less than 0.15wt%, the sodium ion less than 0.1wt%, the sodium ion less than 0.05wt%.Each in these consumptions can be changed by term " about ".

Term used herein " earth material " or " ground " refer to any horizontal surface that people can walk thereon.Earth material or ground can be made up of inorganic material such as ceramic tile or natural stone (for example, clinker tile), or organic material such as epoxy resin, polymer, rubber or elastomeric material.Earth material or ground may be in any or various environment, such as restaurant (such as fast food restaurant), food processing and/or preparation enterprise, slaughterhouse, packing shop, shortening (shortening) production plant, kitchen or like environment.

Wording " coefficient of friction " as used herein and " skid resistance " can be defined according to any one of various Open Standards, such as ASTM standard D-2047, " Static Coefficientof Friction of Polish Coated Floor Surfaces as Measured by theJames Machine ", with ASTM Committee D-21 report, this report shows that the ground according to the confficient of static friction of this test measurement not less than 0.5 is thought to provide non-hazardous walkway surface.NBS Technical Note895 " An Overview of Floor Slip-Resistance; in With AnnotatedBibliography " be proved to be qualified of this numerical value in RobertJ.Brungraber, wherein it shows that 0.5 numerical value provides the coefficient of safety, and the most people of taking normal strides can not possibly slip on surface of the numerical value more than 0.3-0.35.Other related and similar standard includes ANSI 1264.2-2001, ASTMC1028-89, ASTM D2047-93, ASTM F1679-00 (it is related to Britain XL frictional force meter), ASTM methods of test F1677-96 and UL410 (1992).Each standard in this paragraph is incorporated herein by reference.

It is as used herein change the component content used in the present composition or the inventive method term " about " be exponential quantity change, this change in real world for example by preparing concentrate or causing possibility using typical measurement used in solution and material handling procedures, by the mistake made unintentionally in these processes, by preparing the present composition or carrying out the difference and analogue of the preparation of each composition, source or purity used in the method for the present invention.Change in spite of by term " about ", claim includes the equivalent of these contents.

Stable microorganism formulation

The present invention relates to containing borate and the microorganism formulation of the stabilization of microorganism.The microorganism can be spore (bacterium or fungi), bacterial nutrition or fungi form.Microorganism formulation may include the dirt that can for example digest or degrade, the spore or spore blend of such as lubricating grease, oil (such as vegetable oil or animal tallow), protein, carbohydrate or the like.Microorganism formulation can also produce the enzyme of assistant degradation dirt such as lubricating grease, oil, fat, protein, carbohydrate or the like.Borate may include any one of various salt of boric acid, for example some alkali metal salts or alkanol amine salt.The salt of boric acid can give the Cleasing compositions containing stable microorganism formulation to provide alkali source.

Compared with the conventional microorganism formulation for example used in Cleasing compositions, the salt of boric acid can provide microorganism formulation favourable stability.Start have such as 10⁴The conventional microorganism formulation of individual viable bacteria or spore contained only 10 after 4 months³Or even only 10²The organism of individual work.That is, they lose the active organism of 1 or 2 logarithm, this can reduce the amount of contaminants for removing, digesting or degrading.In one embodiment, the microorganism formulation of stabilization of the invention was less than 1 or 2 logarithm in internal loss in 4 months, or less than the activity of 1 logarithm.This provides longer shelf life to the product containing microorganism formulation.

In one embodiment, the microorganism formulation of stabilization of the invention is a component in Cleasing compositions.Although not limiting the present invention, the microorganism formulation can be considered as the source of the detergency enzymes in Cleasing compositions.It is not the extra enzyme being produced in situ in by microorganism formulation that this Cleasing compositions, which may also comprise,.Microorganism formulation can produce such as enzyme, such as protease, lipase and/or amylase.Said composition may also comprise other additional enzymes, such as protease, lipase and/or amylase.Although not limiting the present invention, additional enzyme can be considered as providing if Cleasing compositions are applied to be cleaned immediately, and microorganism formulation is considered as when microorganism is retained on product to be cleaned, or even provides lasting cleaning after rinsing.

Most of cleaning agents can only provide decontamination, and this decontamination only actually is dirt is moved to from a surface or position (such as ground) from another in (such as drainpipe).In some embodiments, the Cleasing compositions of the stable microorganism formulation containing the present invention can provide decontamination simultaneously by the persistent enzymatic breakdown of dirt and lasting dirt declines.The Cleasing compositions of the microorganism formulation of stabilization containing the present invention can be used for various purposes, it is used as grease digester including the combination as ground cleaner, as grout cleaner, as ground and drain cleaner and degreasing agent/grease digester, in grease trap, it is used for efflux and/or wastewater treatment (for example in municipal wastewater processing, reduction fat, oil and grease), it is used as grease digester in oil plant, or for black and grey water process on cruise ships.

Although not limiting the present invention, the lubricating grease on surface or oil decomposition can be made by thinking the microbial composite of the stabilization of the present invention.Decompose lubricating grease or the releasable other dirts adhered in lubricating grease or oil of oil.Therefore, the cleanable surface of composition of the invention.In one embodiment, the present invention relates to the method for the microbial composite of the stabilization including applying the present invention repeatedly.For example, the method for the present invention may include daily application.Apply the surface of 5-14 days cleanable light contaminations.Apply the surface of 3-6 weeks cleanable severe contamination.

The salt of boric acid

The present invention relates to stable microbial cleaning composition, it uses the salt of one or more boric acid to provide the improved stability of microorganism formulation even at basic ph.The salt of suitable boric acid can provide basicity to stable microbial cleaning solution.This salt includes alkali borate；Amine borate, preferably alkanolamine borate；And analog；Or its combination.In some embodiments, the salt of boric acid includes potassium borate, monoethanol ammonium borate, diethanol ammonium borate, three ethanol ammonium borates and analog or its combination.In one embodiment, the salt of boric acid includes monoethanolamine borate.

The salt of boric acid, such as potassium borate or monoethanolamine borate can be obtained by any various routes.For example, commercially available borate, such as potassium borate can be added in composition.Or, can by using alkali, such as such as potassium hydroxide of the alkali containing potassium or such as MEA alkali, neutralize boric acid, so as to obtain borate, such as potassium borate or monoethanolamine borate.

In some embodiments, borate is in the present compositions in concentration more than 5 or 10wt%, such as more than being solvable under 11,12,13,14,15,16,17,18,19 or 20wt%.Borate used in the present composition can be used with the Cmax for being up to its solubility limit.In some embodiments, borate is dissolved under most 35wt%, such as most 25,30 or 35wt% concentration in the composition of the present invention.In some embodiments, borate is solvable under 12-35wt%, 15-30wt% or 20-25wt%, preferably 20-25wt%.The composition of the present invention may also comprise any consumption changed by term " about " or the borate of scope.

In one embodiment, alkanolamine borate, such as monoethanolamine borate are solvable especially under the concentration of Boratex more than other borates.It is solvable that can be used in the Cleasing compositions of the present invention under alkanolamine borate, such as monoethanolamine borate, and under concentration listed above, about preferably up to 30wt%, preferably from about 20 to about 25wt% concentration.In one embodiment, this high-dissolvability can be obtained under alkaline pH, such as pH about 9 to about 10.5.

In one embodiment, potassium borate is solvable under the concentration more than other metal borates, especially alkali borate, particularly Boratex.Potassium borate can be used in the enzymatic cleaning compositions of the present invention, and under concentration listed above, preferably up to about 25wt% is solvable under preferably from about 15 to about 25wt% concentration.In one embodiment, this high-dissolvability can be obtained under alkaline pH, such as pH about 9 to about 10.5.

Compared to being suitable for maintaining pH to be greater than about 7, greater than about 8, about 8 to about 11 or about 9 to about 10.5 other buffer systems, borate may be provided in the increase needed for microbial preparation stability under alkaline pH.Larger cleaning capacity can be provided by maintaining alkaline pH.

The bacteria composition of the stabilization of the present invention can be substantially free of sodium ion.Advantageously, it is solvable under the big concentration of concentration of borate when than there is sodium ion in the composition substantially free of sodium ion.Regrettably, sodium ion is a kind of counter ion counterionsl gegenions of common salt.Therefore, it is necessary to carefully provide the composition of the invention substantially free of sodium ion.For example, can be prepared by the reagent of sour form present invention is substantially free of the composition of sodium ion, wherein optionally neutralizing the reagent of the sour form by alkanolamine or potassium hydroxide.For example, can be by the salt in addition to sodium salt, such as potassium or alkanol amine salt, present invention is substantially free of the composition of sodium ion for preparation.In one embodiment, composition of the invention includes sodium ion, and its content make it that Boratex is not precipitated out from said composition.A kind of mode for realizing the sodium of this low content is not include sodium salt in composition or do not include the sodium salt in addition to amphoteric surfactant.Preferably, or even in the case of using the sodium in amphoteric surfactant, composition of the invention is also substantially free of sodium ion.Present invention is substantially free of the borate that the Cleasing compositions of sodium ion may include concentration most about 35wt% e.g., from about 15 to about 30wt%.In one embodiment, in alkaline pH, such as under pH about 9 to about 10.5, this high-dissolvability is obtained.

Compared to being suitable for maintaining pH to be greater than about 7, greater than about 8, about 8 to about 11 or about 9 to about 10.5 other buffer systems, including borate and the composition substantially free of sodium ion may be provided in the increase under alkaline pH needed for microbial preparation stability.Larger cleaning capacity can be provided by maintaining alkaline pH.

In some embodiments, alkanolamine borate is with about 5 to about 35wt%, about 10wt% to about 30wt%, about 10wt% to about 20wt%, and about 5wt% is to about 15wt%, or about 15wt% to about 25wt% concentration is present.In some embodiments, alkanolamine borate is with the about 5wt% of composition, about about 10wt%, about 15wt%, 20wt%, and about 25wt% or about 30wt% concentration are present.This is matched somebody with somebody can be substantially free of sodium ion.The composition of the present invention may also comprise any consumption or scope without the alkanolamine borate changed with term " about ".

In some embodiments, monoethanolamine borate is with the about 10wt% of composition to about 30wt%, or about 10wt% to about 20wt%, about 5wt% are to about 15wt%, or about 15wt% to about 25wt% concentration is present.In some embodiments, monoethanolamine borate is with the about 5wt% of composition, about about 10wt%, about 15wt%, 20wt%, and about 25wt% or about 30wt% concentration are present.This is matched somebody with somebody can be substantially free of sodium ion.The composition of the present invention may also comprise any consumption or scope without the monoethanolamine borate changed with term " about ".

In some embodiments, borate is with about 5 to about 35wt%, about 10wt% to about 30wt%, about 10wt% to about 20wt%, and about 5wt% is to about 15wt%, or about 15wt% to about 25wt% concentration is present.In some embodiments, borate is with the about 5wt% of composition, about about 10wt%, about 15wt%, 20wt%, and about 25wt% or about 30wt% concentration are present.This is matched somebody with somebody can be substantially free of sodium ion.The composition of the present invention may also comprise any consumption or scope without the borate changed with term " about ".

Microorganism formulation

Any various spores (bacterium or fungi), bacterial nutrition or fungi can be used in the bacteria composition of the stabilization of the present invention.For example, the composition of the present invention may include microorganism or its mixture of any work, the microorganism or its mixture can withstand the formula and the use environment intended, or analog common in the lipid that can digest, degrades or promote to degrade, protein, carbohydrate, other organic matters or family, mechanism and industrial soil or efflux.Many suitable bacterial strains and species are known.

Suitable spore (bacterium or fungi), bacterial nutrition or fungi include bacillus (Bacillus), pseudomonas (Pseudomonas), arthrobacterium (Arthrobacter), enterobacteria (Enterobacter), citric acid bacillus (Citrobacter), bar bacterium (Corynebacter), bacterium nitrobacter (Nitrobacter) its mixture, or the like；Acinetobacter calcoaceticus (Acinetobacter), Aspergillus (Aspergillus), nitrogen-fixing bacteria (Azospirillum), bulkholderia cepasea (Burkholderia), Ceriporiopsis, Escherichia (Escherichia), lactobacillus (Lactobacillus), series bacillus (Paenebacillus), secondary coccus (Paracoccus), Rhodococcus sp (Rhodococcus), Syphingomonas, streptococcus (Streptococcus), Thiobacillus (Thiobacillus), trichoderma (Trichoderma), Xanthomonas campestris (Xanthomonas), lactobacillus (Lactobacillus), nitrosomonas (Nitrosomonas), Alcaliaens, Klebsiella (Klebs iella), its mixture or the like；And its mixture or the like.

Suitable bacillus includes bacillus licheniformis (Bacil luslicheniformis), bacillus subtilis (Bacillus subtilis), bacillus polymyxa (Bacilluspolymyxa) or the like；Methanol bacillus (Bacillus methanolicus), bacillus amyloliquefaciens (Bacillus amyloliquefaciens), Bacillus pasteurii (Bacilluspasteurii), D-lactic acid bacillus (Bacillus laevolacticus), bacillus megaterium (Bacillus megaterium), its mixture or the like；And its mixture or the like.Suitable pseudomonas includes pseudomonas aeruginosa (Pseudomonas aeruginosa), solution alkali pseudomonas (Pseudomonasa lkanolytica), denitrogenation pseudomonas (Pseudomonas dentrificans) or its mixture or the like.Suitable arthrobacterium includes arthrobacter paraffineus (Arthrobacter paraffineus), bites oil arthrobacterium (Arthrobacter petroleophagus), slightly red arthrobacterium (Arthrobacterrubellus), arthrobacterium sp., or its mixture or the like.Suitable enterobacteria includes enterobacter cloacae (Enteroba ctercloacae), Enterobacter sp., or its mixture or the like.Suitable citric acid bacillus are included without malonic acid citric acid bacillus (Citrobacteramalonaticus), citrobacter freundii (Citrobacter freundi), or its mixture or the like.Suitable bar bacterium includes Corynebacterium al kanum, Corynebacterium fujiokense, Corynebacterium hydrocarboxydano, bar bacterium sp., its mixture or the like.

Suitable spore (bacterium or fungi), bacterial nutrition or fungi include those numbered with ATCC：21417th, 21424,27811,39326,6051a, 21228,21331,35854,10401,12060,21551,21993,21036,29260,21034,13867,15590,21494,21495,21908,962,15337,27613,33241,25405,25406,25407,29935,21194,21496,21767,53586,55406,55405,55407,23842,23843,23844,23845,6452,6453,11859,23492, its mixture or the like.

The suitable microorganism that can be used in the present invention is included in United States Patent (USP) Nos.4655794,5449619 and 5863882；With those described in U.S. Patent Publication Nos.20020182184,2003012688 and 20030049832, the disclosure of which is incorporated herein by reference.

Suitable spore (bacterium or fungi), bacterial nutrition or fungi are commercially available in various sources (for example, Sybron Chemicals, Inc., Semco Laboratories, Inc., or Novozymes).The trade name of this product includes SPORZYME

1B、SPORZYME

UltraBase2、SPORZYME

EB、SPORZYME

BCC、SPORZYME

WC Wash、SPORZYME

FE、BI-CHEM

MSB、BI-CHEM

Purta Treat、BI-CHEM

BDO、BI-CHEM

SANI-BAC

、BI-CHEM

BIO-SCRUB

、BI-CHEM

GC600L

、BI-CHEM

Bioclean、GREASE GUARD

Or the like.

In one embodiment, spore (bacterium or fungi), bacterial nutrition or fungi include being particularly suitable for the Bacillus strain of high yield ectoenzyme, especially protease, amylase and cellulase.This bacterial strain is common in waste treatment products.This mixture may include bacillus licheniformis, bacillus subtilis and bacillus polymyxa.As further example, Bacillus pasteurii can show high-caliber lipase production；D-lactic acid bacillus can show germination circulation faster；Bacillus amyloliquefaciens can show high-caliber protease production.

Include about 1 × 10 in the formula endospore (bacterium or fungi), bacterial nutrition or the suitable concentration of fungi³To about 1 × 10⁹CFU/ml, about 1 × 10⁴To about 1 × 10⁸CFU/ml, about 1 × 10⁵To about 1 × 10⁷CFU/ml, or the like.Effective cleaning amount can be used in the present compositions, e.g., from about 0.5 to about 10wt%, about 1 to about 5 (such as 4) wt%, about 2 to about 10wt%, about 1 to about 3wt% or about 2wt% spore (bacterium or fungi), bacterial nutrition or the commercially available composition of fungi.The present composition may include not with these consumptions or scope about changed.

The embodiment of stable microorganism formulation

In one embodiment, the microorganism formulation of the stabilization of the present invention includes microorganism formulation (for example, bacteria preparation, such as spore blend), borate (for example, alkanolamine borate, such as monoethanolamine borate) and optional polyalcohol (for example, propane diols).In some embodiments, the microorganism formulation of the stabilization of the present invention includes about 2 to about 40wt% borates, about 3 to about 15wt% borates, about 5 to about 30wt% borate, about 5 to about 25wt% borate, about 5 to about 10wt% borates, about 10 to about 15wt% borates or about 25 to about 30wt% borate.In some embodiments, composition of the invention includes about 2 to about 30wt% polyalcohol, about 2 to about 10wt% polyalcohol, about 5 to about 20wt% polyalcohol, about 5 to about 10wt% polyalcohol, or about 10 to about 20wt% polyalcohol.In some embodiments, the microorganism formulation of the stabilization of the present invention includes about 2 to about 40wt% polyalcohol, about 2 to about 20wt% polyalcohol, about 2 to about 15wt% polyalcohol, about 2 to about 10wt% polyalcohol, or about 3 to about 10wt% polyalcohol, about 4 to about 15wt% polyalcohol or about 4 to about 8wt% polyalcohol, the polyalcohol of about 4wt% polyalcohol, about 8wt%, or about 12wt% polyalcohol.In some embodiments, the microorganism formulation of the stabilization of the present invention includes about 10 to about 95wt% water, about 15 to about 75wt% water, about 15 to about 35wt% water, about 25 to about 75wt% water, about 40 to about 70wt% water, about 45 to about 65wt% water, or most about 50, about 55, about 60, about 65, or about 70wt% water.

In one embodiment, Cleasing compositions of the invention include spore, bacterium or fungi, and alkanolamine borate.In one embodiment, composition can have the pH more than or equal to 9, e.g., from about 9 to about 10.5.In one embodiment, composition can have the pH more than or equal to 8, e.g., from about 8 to about 9.5.Said composition may also comprise polyalcohol.In one embodiment, polyalcohol may include propane diols.Said composition also includes up to about 65wt% water.

In one embodiment, alkanolamine borate may include monoethanol ammonium borate, diethanol ammonium borate, triethanolamine ammonium borate or its combination.Composition may include about 5 to about 35wt% alkanolamine borate, about 10 to about 30wt% alkanolamine borate, or about 15 to about 25wt% alkanolamine borate.

In one embodiment, Cleasing compositions of the invention include spore, bacterium or fungi, and borate, and can be substantially free of sodium ion.Composition can have the pH more than or equal to 9, e.g., from about 9 to about 10.5.Composition may also comprise polyalcohol.In one embodiment, polyalcohol may include propane diols.Composition also includes up to about 65wt% water.

Borate may include potassium borate.Potassium borate may include the combination of potassium hydroxide and boric acid.Composition may include about 5 to about 35wt% borate, about 10 to about 30wt% borate, or about 15 to about 25wt% borate.

In one embodiment, spore or bacterium may include bacterial spore.

Cleasing compositions containing stable microorganism formulation

The invention further relates to the Cleasing compositions of the microorganism formulation of the stabilization containing the present invention.In one embodiment, concentrate of the invention and the aqueous cleaning composition of dilution may include the blending surfactant containing nonionic surfactant and silicone surfactant of valid density, and the stabilization of the present invention microorganism formulation.These compositions may also comprise anion surfactant and hydrotropic agent or solubilizer, and it can maintain the aqueous solution or suspension of single-phase non-separation.The microorganism formulation of the stabilization of the present invention is disclosed in United States Patent (USP) Nos.6425959 and 6506261 may include the suitable Cleasing compositions in it, and the disclosure of which is incorporated herein by reference.

In one embodiment, composition and method may include nonionic surfactant and nonionic siloxane surfactant.Said composition may also comprise anion surfactant and hydrotropic agent (it can be the anionic compound with seldom surfactant character), such as amine oxide material.The pure this composition without diluent can be used, to remove the oily or greasy organic soils and mineral contaminants of complexity from typically hard metal or other hard surfaces.Said composition can obtain excellent clean-up performance containing alkali source and enough blends.

In one embodiment, Cleasing compositions of the invention (concentrate or dilutable liquid) may include about 0.003 to the about 70wt% blending surface activator composition containing nonionic surfactant and nonionic siloxane surfactant.Nonionic surfactant can be free of oxyalkylene segment, can be block (EO) (PO) copolymer, alcohol alkoxide, alkyl phenol alkoxylate, or amine alkoxide, wherein alkoxide is (EO) or (PO) part.The weight ratio of nonionic surfactant and nonionic siloxane surfactant can be that every 1 parts by weight silicone surfactant or its blend are about 1 to about 10 parts by weight, preferably 3-7 portions of nonionic surfactant or its blend.This composition may also comprise about 0.003 to about 35wt% one or more anion surfactants, about 0.001 to the about 20wt% effective hydrotropic agent of one or more, or its mixture.Hydrotropic agent can be alkyl dimethyl amine oxide.Hydrotropic agent can maintain chelating agent and surfactant blend to be in uniform single phase aqueous composition.

In one embodiment, the concentrate composition of the present invention may include about 1 to about 15wt% one or more nonionic siloxane surfactants, about 5 to about 75wt% one or more nonionic surfactants, about 5-75wt% one or more anion surfactants, about 2-20wt% one or more hydrotrope solubilizers (for example, amine oxide material).In this embodiment, the ratio between nonionic surfactant and nonionic siloxane surfactant can be that every 1 parts by weight nonionic siloxane surfactant is about 3 to about 7 parts by weight nonionic surfactants.

In the embodiment of the aqueous compositions formulated of dilution, the aqueous solution may include about 0.0005 to about 35wt% or about 0.1 to about 10wt% silicone surfactant, about 0.0003-35wt% or about 0.3-30wt% nonionic surfactant, about 0.003-35wt% or about 0.3-30wt% anion surfactant, about 0.001-20wt% or 0.2 to about 30wt% hydrotrope solubilizers, while maintaining the ratio between nonionic listed above and silicone surfactant.

In one embodiment, wiping concentrate can include about 0.003-35wt% or about 0.1-25wt% chelating agent or sequestering agent in aqueous base；About 0.003-35wt% or about 0.3-30wt% nonionic surfactant；About 0.0005-35wt% or about 0.01-10wt% nonionic siloxane surfactant；About 0.003-30wt% anion surfactant；About 0.001-20wt% or about 0.2-30wt% hydrotropic agent or surface active agent solubilization agent (for example, amine oxide).

It can be used pure wiping concentrate, or available water is to be enough to obtain the dilution proportion of activity aqueous cleaning agent of the dilution listed above wiping concentrate.In the context of the present invention, term " pure " refers to be substantially absent from diluent, such as water-bearing media.The cleaning agent of gained dilution can be applied in the substrate of pollution for decontamination.

For the purpose of present patent application, the Cleasing compositions may include chelating agent, nonionic/nonionic siloxane surfactant blend, anion surfactant and hydrotropic agent (such as amine oxide).This embodiment can be used for decontaminating from corrosion resistant surface.Chelating agent can be sylvite.Similarly, hydrotropic agent can be sylvite.

The embodiment of Cleasing compositions

In some embodiments, the Cleasing compositions of the present invention can be described by the composition and consumption listed in following table.The composition in stable microbial composite is not listed in the following table, but is existed as described above.It also can use these consumptions or scope in about change table.

Concentrate composition

 

Chemicals	Wt%	Wt%	Wt%
				Chelating agent	0-30	0.5-15	0.5-15
Silicone surfactant	0.1-35	0.1-10	1-7
				Nonionic surfactant	0.5-35	1-20	1-15
Anion surfactant	0.5-35	1-20	1-15
				Hydrotropic agent	0.1-20	0.5-15	0.5-10

 

Chemicals	Wt%	Wt%
			Chelating agent	0.1-30	0.5-15
Surfactant blend	0.5-70	1-30
			Anion surfactant	0.1-70	0.5-35
Amine oxide hydrotropes	0.1-20	0.5-15
			Optional acid	To >=pH9	To >=pH10

 

Chemicals	Wt%	Wt%	Wt%	Wt%	Wt%
						Nonionic surfactant	2-16	4-16	2-8	8	4
Silicone surfactant	0.5-6	1-6	0.5-2	3	1
						Amphoteric surfactant	1-10	2-10	1-6	5	3
Anion surfactant	2-16	4-16	2-8	8	4
						Hydrotropic agent	1-20	5-20	1-6	11	3

The Aquo-composition (using as former state or as formulation additives) of dilution

 

Chemicals	ppm	ppm	ppm
				Chelating agent	0-150,000	60-20,000	1200-10,000
Surfactant blend	30-175,000	3000-100,000	6000-50,000
				Anion surfactant	30-175,000	3000-100,000	6000-50,000
Hydrotropic agent	10-100,000	1000-60,000	2000-20,000
				Aqueous diluent and stable microbial composite	Surplus	Surplus	Surplus

 

Chemicals	ppm	ppm
			Chelating agent	6-70,000	600-20,000
Surfactant blend	30-350,000	3000-100,000
			Anion surfactant	30-350,000	3000-100,000
Amine oxide hydrotropes	7-80,000	700-25,000
			Optional acid	To >=pH9	To >=pH10
Aqueous diluent and stable microbial composite	Surplus	Surplus

Upper table indicates the useful composition for Cleasing compositions of the present invention.The table lists the consumption of some compositions, and the microbial composite of stabilization of the invention also includes spore, bacterium or fungi and borate.This composition can be used as organic dirt or grease removers.Surfactant blend listed above refers to the combination of the nonionic and silicone nonionic surfactant of the above ratio.In addition, chelating agent is useful, but it is not necessarily to.Chelating agent provides chelating and decontaminated, but can help to the corrosion to some surfaces or other chemical damages.

In one embodiment, Cleasing compositions of the invention include spore, bacterium or fungi, and borate, such as alkanolamine borate.In some embodiments, said composition may also comprise about 0.003 to about 35wt% nonionic surfactant, e.g., from about 0.5 to about 35wt% nonionic surfactant.Nonionic surfactant may include containing at least (EO)_y(PO)_zNon-ionic block copolymer, wherein y and z independently are 2 to 100；C with 2-15mol oxirane_6-24Alkyl phenol alkoxylate；C with 2-15mol oxirane_6-24Alcohol alkoxide；Alkoxylated amines with 2-20mol oxirane；Or its mixture.

In some embodiments, said composition may also comprise about 0.0005 to about 35wt% silicone surfactant, e.g., from about 0.1 to about 35wt% silicone surfactant.The silicone surfactant may include the epoxy alkyl of siloxane main chain and at least one pendant with about 2-100mol alkylene oxides.The epoxy alkyl of pendant may include (EO)_n, wherein n is 3-75.

In some embodiments, said composition may also comprise about 0.003 to about 35wt% anion surfactant, e.g., from about 0.5 to about 35wt% anion surfactant.Anion surfactant may include linear alkylbenzene sulfonate (LAS)；Alhpa olefin sulfonate；Sulfuric acid alkyl ester；Secondary paraffin sulfonate；Sulfosuccinate；Or its mixture.Anion surfactant may include alkanol ammonium alkylbenzenesulfonate.Anion surfactant may include monoethanol ammonium alkylbenzenesulfonate.

In some embodiments, said composition may also comprise about 0.001 to about 20wt% hydrotropic agent, e.g., from about 0.1 to about 20wt% hydrotropic agent.Hydrotropic agent may include C_6-24Alkyl dimethyl amine oxide；Alkylated dipheny ethers disulfonate；Or its mixture.Hydrotropic agent may include amine base amine oxide surfactant.Hydrotropic agent may include different C_10-14Alkyl dimethyl amine oxide.Hydrotropic agent may include alkylated dipheny ethers disulfonic acid or its salt.

In one embodiment, said composition may also comprise about 0.5 to about 35wt% nonionic surfactant and about 0.1 to about 35wt% silicone surfactant.In this embodiment, nonionic surfactant may include containing at least (EO)_y(PO)_zNon-ionic block copolymer；C with 2-15mol oxirane_6-24Alkyl phenol alkoxylate；C with 2-15mol oxirane_6-24Alcohol alkoxide；Alkoxylated amines with 2-20mol oxirane；Or its mixture.In this embodiment, silicone surfactant may include the epoxy alkyl of siloxane main chain and at least one pendant with about 2-100mol alkylene oxides.

In this embodiment, the weight ratio of nonionic surfactant and nonionic siloxane surfactant can be that every 1 part of silicone surfactant is the nonionic surfactant of about 0.1 to about 10 parts by weight.In one embodiment, the weight ratio of nonionic surfactant and nonionic siloxane surfactant can be that every 1 part of silicone surfactant is the nonionic surfactant of about 3 to about 7 parts by weight.

In some embodiments, composition may also comprise about 0.5 to about 35wt% nonionic surfactant, about 0.1 to about 35wt% silicone surfactant, about 0.5 to about 35wt% anion surfactant, and about 0.1 to about 20wt% hydrotropic agent.

Composition in stable microorganism formulation

The microorganism formulation and/or Cleasing compositions of the stabilization of the present invention may include to can be used for any one of various composition of cleaning or other purposes.This composition may include enzyme, surfactant, hydrotropic agent, chelating agent, bivalent cation, polyalcohol, aesthetic enhancing agent, solvent, preservative or the like.

In some embodiments, said composition may also comprise one or more solvents of effective dose；One or more enzymes of effective dose；One or more antiseptics of effective dose；One or more chelating agents of effective dose；Or its mixture.Said composition may include about 0.1-30wt% chelating agent.The chelating agent may include small molecule or polymerizable compound with carboxyl, or its mixture.

Enzyme may include detergency enzymes.Detergency enzymes may include protease, amylase, lipase, cellulase, peroxidase, glucose enzyme (gluconase) or its mixture.Detergency enzymes may include alkali protease, lipase, amylase or its mixture.

In some embodiments, said composition may also comprise calcium ion source, polyalcohol, builder, dyestuff or its conjugate or mixture.

Surfactant

The surfactant of the present invention or the mixture of surfactant may be selected from water-soluble or the dispersible nonionic of water, Semi-polar nonionic, anion, cation, both sexes or amphiphilic surfactant；Or its any combination.To may depend on final use condition using the specific surfactant or the mixture of surfactant that select in the method for the present invention and product, including preparation method, physical product form, pH, temperature in use, foam control and soil type are used.The surfactant being incorporated into the Cleasing compositions of the present invention is preferably compatible with enzyme, is not the substrate of enzyme in composition, is not the inhibitor or deactivator of enzyme.For example, when using protease and amylase in the present compositions, surfactant is preferably free of peptide and glycosidic bond.Additionally, it is known that some cationic surfactants reduce the effect of enzyme.

In general, the surfactant or the concentration of surfactant mixture that can be used in the composition of the stabilization of the present invention are about the 0.5% to about 40% of composition weight, preferably from about 2% to about 10%, preferably from about 5% to about 8%.These percentages can refer to the percentage of commercially available surface activator composition, and the surface activator composition can also contain solvent, dyestuff, flavoring agent and analog in addition to containing actual surfactant.In this case, the percentage of actual surface activating agent chemicals can be less than listed percentage.These percentages can refer to the percentage of actual surface activating agent chemicals.

Nonionic surfactant

Available for the present invention nonionic surfactant be generally characterised in that exist organic hydrophobic group and organic hydrophilic base and typically via be condensed organic aliphatic series, alkyl aromatic or polyoxyalkylene hydrophobic compound and hydrophilic-basic oxide portions (in common practice, this hydrophilic-basic oxide portions is oxirane or its polyhydrate product, polyethylene glycol) and produce.In practice, any hydrophobic compound with hydroxyl containing hydrogen atoms, carboxyl, amino or amide groups can form nonionic surfactant with oxirane or its many hydration adduct, or its mixture condensation with alkylene oxide such as expoxy propane.The length for the hydrophilic polyoxyalkylene part that can be condensed with any specific hydrophobic compound can be easily adjusted, obtains having required hydrophilic and hydrophobic performance balanced degree water dispersible or water soluble compound.

EOPO nonionic surfactants

In the homopolymer or embedding disconnected or miscellaneous copolymer of graft, the example with the useful nonionic surfactant that silicone surfactant is used together is by the polyether compound of oxirane, expoxy propane preparation.This polyether compound is referred to as polyalkylene oxide polymer, polyoxyalkylene polymers or polyalkylene glycol polymers.The molecular weight ranges of this nonionic surfactant are about 500 to about 15,000.It was found that some type of polyoxypropylene-polyoxyethylene glycol polymer nonionic surfactant is particularly useful.Can be used containing at least one polyoxypropylene it is embedding disconnected and with least one be connected to the polyoxypropylene it is embedding it is disconnected on polyoxyethylene other embedding disconnected surfactants.Embedding break of extra polyoxyethylene or polyoxypropylene may be present in an intramolecular.These materials that average molecular weight range is about 500 to about 15,000 are with PLURONIC

Commercially available from (being prepared by BASF Corporation) form, and with the various other trade name forms acquisition of their chemical supplier.In addition, PLURONIC

R (the PLURONIC structures of reversion) can also be used in the composition of the present invention.In addition, epoxy alkyl, aliphatic acid or the other this groups being used together with alcohol with alkylphenol can be used.Useful surfactant may include the poly-alkoxylation C of end-blocking_6-24Straight chain alcohol.Surfactant can be prepared using polyoxyethylene or polyoxypropylene units, and can be allowed to block using the common agents for forming ether end group.Useful this surfactant materials are (PO)_xCompound or benzyl ether compound poly-ethylene oxide C_12-14Straight chain alcohol；Referring to United States Patent (USP) No.3444247.The polyoxypropylene polyoxyethylene block polymer being particularly useful be the embedding Duan Hegai centers in center containing polyoxypropylene units it is embedding it is disconnected per side on polyoxyethylene units embedding disconnected those.

These copolymers have chemical formula as shown below：

(EO)_n-(PO)_m-(EO)_n

Wherein m is integer 21-54；N is integer 7-128.Extra useful block copolymer is the embedding disconnected block polymer of the polyoxypropylene units on the embedding disconnected every side in the embedding Duan Hegai centers in center with polyoxyethylene units.The copolymer has chemical formula as shown below：

(PO)_n-(EO)_m-(PO)_n

Wherein m is integer 14-164, and n is integer 9-22.

A kind of suitable nonionic surfactant used in the compositions of the present invention includes the alkyl phenol alkoxylate of following formula：

Wherein R ' includes C_2-24Aliphatic group and AO represent Oxyranyle, expoxy propane base, hybrid EOPO bases or embedding disconnected EO-PO, PO-EO, EOPOEO or POEOPO base, and Z represents H or (AO), benzyl or other end groups.Suitable nonionic surfactant includes the alkyl phenol ethoxylate of following formula：

Wherein R¹Including C_6-18Aliphatic group, preferably C_6-12Aliphatic group, and n are integers about 2 to about 24.The principle example of this surfactant is the nonylphenol ethoxylate in b-oxide base with 2.5-14.5molEO.(PO) can be used_xBase (x is 2.5-12.5) blocks b-oxide base, or is allowed to benzyl moiety to block.

Alkoxylated amines

The composition of the present invention may include any one of various alkoxylated amines.In one embodiment, alkoxylated amines have formula I：N(R₁)(R₂)(R₃)(R₄), wherein R₁、R₂Or R₃In at least one include alkoxide or ether moiety.R₄Can be hydrogen, straight or branched alkyl, or straight or branched alkaryl.Alkoxylated amines can be primary, secondary or tertiary amine.In one embodiment, alkoxylated amines are tertiary amines.In some embodiments, R₂And R₃In each include alkoxide part, such as one or more ethoxylate moieties, one or more propoxylate moieties, or its combination, and R₄It is hydrogen.For example, R₁、R₂Or R₃One of may include ether moiety and other two may include one or more ethoxylate moieties, one or more propoxylate moieties or its combination.

As further example, alkoxylated amines can be represented with formula IIa, IIb or IIc respectively：

IIa              R⁵-(PO)_sN-(EO)_tH

IIb              R⁵-(PO)_sN-(EO)_tH(EO)_uH, and

IIc              R⁵-N(EO)_tH；

Wherein R₅Can be alkyl, alkenyl or the other aliphatic groups of 8-20 or 12-14 carbon atom, or alkaryl, EO is oxygen ethylidene, and PO is oxygen propylidene.S is 1-20,2-12 or 2-5；T is 1-20,1-10,2-12 or 2-5；It is 1-20,1-10,2-12 or 2-5 with u.Other variants in these compound ranges can be represented with Formula Il d：

R⁵-(PO)_v-N[(EO)_wH][(EO)_zH]

Wherein R⁵As defined above, v is 1-20 (for example, 1,2,3 or 4, or in one embodiment for 2), w and z independently is 1-20,1-10,2-12 or 2-5.

In one embodiment, alkoxylated amines are ether amines alkoxides.Ether amines alkoxide can have Formula Il I：

In formula III, R¹Can be straight or branched alkyl or alkaryl；R²The alkyl of hydrogen or 1-6 carbon can independently be in either case；R³The alkyl of hydrogen or 1-6 carbon can independently be in either case；M is flat to may each be about 1 to about 20；X and y each can independently average out to 1 to about 20；X+y is flat to may each be about 2 to about 40.

In one embodiment, in formula III, R¹Can be：The alkyl of 8-24 carbon atom, alkaryl, and contain about 7 to about 30 carbon atoms, or alkaryl (for example, two substitutions have the alkaryl of alkyl)；R²Can be containing 1 or 2 carbon atom, or can be hydrogen；R³Can be hydrogen, the alkyl containing 1 or 2 carbon；X+y's can range from about 1 to about 3.

This ether amines alkoxide is disclosed in United States Patent (USP) Nos.6060625 and 6063145.

In one embodiment, in formula III, R¹Can be：The alkyl of 6-24 carbon atom, alkaryl and contains about 7 to about 30 carbon atoms, or alkaryl (for example, two substitutions have the alkaryl of alkyl)；R²Can be containing 1 or 2 carbon atom, or can be hydrogen；R³Can be hydrogen, the alkyl containing 1 or 2 carbon；X+y's can range from about 1 to about 20.

In one embodiment, in formula III, m can be 0 to about 20, x and y independently of one another put down may each be 0 to about 20.In some embodiments, alkoxy portion epoxy available ethane, expoxy propane or epoxybutane unit end-blocking or closing.

In one embodiment, in formula III, R¹Can be C₆-C₂₀Alkyl or C₉-C₁₃Alkyl, such as straight chained alkyl；R²Can be CH₃；M can be about 1 to about 10；R³Can be hydrogen, x+y's can range from about 5 to about 12.

In one embodiment, in formula III, R¹Can be C₆-C₁₄Alkyl or C₇-C₁₄Alkyl, such as straight chained alkyl；R²Can be CH₃；M can be about 1 to about 10；R³Can be hydrogen, x+y's can range from about 2 to about 12.In one embodiment, this ether amines alkoxide may include the alkoxide part blocked with expoxy propane or epoxybutane unit, and this alkoxide part can provide low foam compositions.

In one embodiment, in formula III, R¹Can be C₆-C₁₄Alkyl, such as straight chained alkyl；R²Can be CH₃；M can be about 1 to about 10；R³Can be hydrogen, x+y's can range from about 2 to about 20.

In one embodiment, alkoxylated amines can be C₁₂-C₁₄Propoxy amine ethoxylate, wherein in formula III, R¹Can be C₁₂-C₁₄Alkyl, such as straight chained alkyl；R²Can be CH₃；M can be about 10；R³Can be hydrogen；X can be that about 2.5, y can be about 2.5.

In one embodiment, alkoxylated amines can be C₁₂-C₁₄Propoxy amine ethoxylate, wherein in formula III, R¹Can be C₁₂-C₁₄Alkyl, such as straight chained alkyl；R²Can be CH₃；M can be about 5；R³Can be hydrogen；X can be that about 2.5, y can be about 2.5.

In one embodiment, alkoxylated amines can be C₁₂-C₁₄Propoxy amine ethoxylate, wherein in formula III, R¹Can be C₁₂-C₁₄Alkyl, such as straight chained alkyl；R²Can be CH₃；M can be about 2；R³Can be hydrogen；X can be that about 2.5, y can be about 2.5.

In one embodiment, in formula III, R¹Can be side chain C₁₀Alkyl；R²Can be CH₂；M can be 1；R³Can be hydrogen；X+y can be about 5.This alkoxylated amines can be the tertiary ethoxylated amine of referred to as poly- (5) oxygen ethylidene isodecyl epoxide propylamine.

In one embodiment, alkoxylated amines can be secondary ethoxylated amine, and it can be represented with following chemical formula：R-(PO)-N-(EO)_x, wherein x=1-7mol oxirane.

In one embodiment, alkoxylated amines can be diamines, and it can be represented with following chemical formula：R-O-CH₂CH₂CH₂N(H)(CH₂CH₂CH₂NH₂), wherein R is, for example, side chain C₁₀Alkyl.

In one embodiment, the ether amines alkoxide of formula III is following formula I V ether amines ethoxylate propoxylate：

In formula IV, R⁶Can be straight or branched alkyl or alkaryl；A is flat to may each be about 1 to about 20；X and y put down may each be about 0 to about 10 independently of one another；X+y is flat to may each be about 1 to about 20.This ether amines alkoxide can be described as ether amines ethoxylate propoxylate.In some embodiments, alkoxy portion can use oxirane, expoxy propane or epoxybutane unit end-blocking or closing.

In one embodiment, alkoxylated amines can be C₁₂-C₁₄Propoxy amine ethoxylate, it can be represented with following chemical formula：R-(PO)₂N[EO]_2.5-H[EO]_2.5-H.In one embodiment, alkoxylated amines can be C₁₂-C₁₄Propoxy amine ethoxylate, it can be represented with following chemical formula：R-(PO)₁₀N[EO]_2.5-H[EO]_2.5-H.In one embodiment, alkoxide can be C₁₂-C₁₄Propoxy amine ethoxylate, it can be represented with following chemical formula：R-(PO)₅N[EO]_2.5-H[EO]_2.5-H.In one embodiment, alkoxylated amines can be known as the tertiary ethoxylated amine of poly- (5) oxygen ethylidene isodecyl oxygen propylamine, and it has the side chain C away from ether oxygen₁₀H₂₁Alkyl.In one embodiment, alkoxylated amines can be diamines, and it can be represented with following chemical formula：R-O-CH₂CH₂CH₂N(H)(CH₂CH₂CH₂NH₂), wherein R is side chain C₁₀Alkyl.In one embodiment, alkoxylated amines can be known as the tertiary ethoxylated amine of iso- (2- ethoxys) isodecyl oxygen propylamine, and it has the side chain C away from ether oxygen₁₀H₂₁Alkyl.

Ether amines alkoxide is for example commercially available with trade name Surfonic (Huntsman Chemical) or Tomah Ether or Ethoxylated Amines.

In one embodiment, alkoxylated amines are Alkyl amine alkoxylates.Suitable Alkyl amine alkoxylates can have lower Formula V：

In Formula V, R¹Can be straight or branched alkyl or alkaryl；R³The alkyl of hydrogen or 1-6 carbon can independently be in either case；X and y each can independently average out to 0 to about 25；X+y is flat to may each be about 1 to about 50.In one embodiment, in Formula V, x and y each can independently average out to 0 to about 10；X+y is flat to may each be about 1 to about 20.In one embodiment, alkoxy portion can use oxirane, expoxy propane or epoxybutane unit end-blocking or closing.

In one embodiment, the Alkyl amine alkoxylates of Formula V are the alkyl amine ethoxylate propoxides of lower Formula IV：

In Formula IV, R⁶Can be straight or branched alkyl or alkaryl (for example, C18 alkyl)；X and y each can independently average out to 0 to about 25；X+y is flat to may each be about 1 to about 50.In one embodiment, in Formula IV, x and y each can independently average out to 0 to about 10 or 20；X+y is flat to may each be about 1 to about 20 or 40.This ether amines alkoxide is referred to alternatively as amine ethoxylate propoxylate.

A kind of such alkyl amine ethoxylate propoxide can use following chemical name N, double -2 (Ω-hydroxypolyoxyethylene/polyoxypropylene) ethyl alkylamines of N- or N, double (polyoxyethylene/propylidene) butter alkanamines of N- (its No. CAS is 68213-26-3), and/or chemical formula C₆₄H₁₃₀O₁₈Represent.

Alkyl amine alkoxylates are for example commercially available with trade name Armoblen (Akzo Nobel).Armoblen600 is referred to as alkyl amine ethoxylate propoxide.

In one embodiment, alkoxylated amines are ether amines.Suitable ether amines can have formula VII：N(R₁)(R₂)(R₃), wherein R₁、R₂Or R₃In at least one include ether moiety.In one embodiment, R₁Including ether moiety and R₂, R₃It is hydrogen.This ether amines can have chemical formula VIII：

R₄O(R₅)NH₂

In chemical formula VIII, R₄Can be C₁-C₁₃Aralkyl or alkyl, straight or branched, R₅Can be C₁-C₆Alkyl, straight or branched.

Ether amines can for example be available commercially from Tomah³ Products。

Suitable alkoxylated amines may include the amine of referred to as ethoxylated amine, the third amine oxide, ethoxy-c amine oxide, alkyl amine alkoxylate, ethoxyquin alkylamine, the third oxidation of alkyl amine, ethoxy-c oxidation of alkyl amine, ethoxy-c oxidation quaternary ammonium compound, ether amines (primary, secondary or uncle), ether amines alkoxide, ether amines b-oxide, ether amines propoxide, alkoxide ether amines, alkyl ether amine alkoxylate, alkyl propoxyl group amine alkoxide, alkyl alkoxy ether amines alkoxide etc..

Extra nonionic surfactant

Extra useful nonionic surfactant includes in the present invention：

1mol has the saturation or unsaturation, straight or branched carboxylic acid of about 8 to about 18 carbon atoms and about 6 to about 50mol oxirane condensation product.Acid moieties can be made up of the sour mixture in carbon atom range defined above, or it can be made up of the acid with specific carbon number within the range.The example of this chemical commercial compound is with trade name Nopalcol

(being prepared by Henkel Co rporation) and Lipopeg

(being prepared by LipoChemicals, Inc.) commercially obtains.

In addition to Ethoxylated carboxylic acids, commonly called macrogol ester, it can be applied in the present invention in special embodiment by the other alkanoates formed with glyceride, glycerine and polyalcohol (sugar or anhydro sorbitol/sorbierite) reaction, in especially indirect food additives application.All these ester moieties have one or more reactive hydrogen sites on its molecule, and the site can undergo further acylation or oxirane (alkoxide) addition, to control the water-wetness of these materials.When adding these fatty esters or acylated carbohydrate into the present composition containing amylase and/or lipase, it is necessary to carefully carry out, because they are potentially incompatible.

The example of the surfactant of nonionic low ratio foamed includes above-described nonionic surfactant, the surfactant is modified by making (multifunctional part) terminal hydroxy group " end-blocking " or " end-block ", will pass through and small hydrophobic molecule such as expoxy propane, epoxy butane, benzyl chloride reaction reduction foaming；And including short chain fatty acids, alcohol or alkyl halide containing 1 to about 5 carbon atom；And its mixture.Equally including reactant, such as thionyl chloride, terminal hydroxy group is changed into chloride group by it.This modification to terminal hydroxy group can cause complete embedding disconnected, embedding mixed, mixed embedding copolymerization or the nonionic surfactant mixed entirely.

The polyhydroxy fatty acid amide for being suitable for using in the present compositions includes having formula R²CONR¹Those of Z, wherein R¹It is H, C₁-C₄Alkyl, 2- ethoxys, 2- hydroxypropyls, ethyoxyl, propoxyl group or its mixture；R²It is C₅-C₃₁Alkyl (it can be straight chain)；It is polyhydroxy alkyl (it has the straight-chain alkyl that at least three hydroxyl is directly connected on the chain) with Z, or its oxidation alkyl derivative (preferably ethoxyquin or the third oxidized derivatives).Z can be derived from the reduced sugar in reductive amination process；Such as glycidyl (glycityl) part.

The suitable nonionic alkyl polysaccharide surfactant for being used especially for the present composition is included in those authorized disclosed in Llenado United States Patent (USP) No.4565647 on January 21st, 1986.These surfactants include the hydrophobic grouping containing about 6 to about 30 carbon atoms, and polysaccharide, such as glycan glycosides, the hydrophilic radical containing about 1.3 to about 10 sugar units.Any reduced sugar containing 5 or 6 carbon atoms, such as glucose, galactolipin, and galactosyl moieties can be used to be replaced with glucosyl moieties.(optionally, hydrophobic grouping is connected in positions such as 2-, 3-, 4-, so as to obtain the glucose or galactolipin relative with glucoside or galactoside).Interior sugared key can be for example in a position of extra sugar unit and between 2-, 3-, 4- and/or 6- position of sugar unit above.

Being suitable for the fatty acid amide surfactant of the present composition includes having chemical formula R⁶CON(R⁷)₂Those, wherein R⁶It is the alkyl containing 7-21 carbon atom, and every R⁷It independently is hydrogen, C₁-C₄Alkyl, C₁-C₄Hydroxyalkyl or-(C₂H₄O)_xH, wherein x scope are 1-3.

By Marcel Dekker, Inc., New York, 1983 publish, Schick, the paper of volume 1 for the Surfactant Science Series that M.J. is editedNonionic SurfactantsIt is a good reference book in terms of the various non-ionic compounds of wide scope usually used in present invention practice.The typical case that nonionic group and these surfactant materials are given in the United States Patent (USP) No.392678 that on December 30th, 1975 authorizes Laughlin and Heuring enumerates." giving further example in Surface Active Agentsand Detergents " (VolI and II, Schwartz, Perry and Berch).

Semi-polar nonionic surfactants

The nonionic surfactant of semi-polar type is another group of nonionic surfactant available for the present composition.In general, semi-polar nonionic surfactants are infusion occurred frequently and foam stabiliser, and this can limit their applications in CIP systems.However, in the composition embodiment of the invention designed for high foam cleaning methodology, semi-polar nonionic surfactants have quick effect.Semi-polar nonionic surfactants include amine oxide, phosphine oxide, sulfoxide and its oxidation alkyl derivative.

Amine oxide corresponds to the tertiary amine oxide of following formulas：

Wherein arrow is the conventional expressing method of semi-polar bond；R¹、R²And R³Can be aliphatic series, aromatics, heterocycle, alicyclic or its combination.For detergent applications amine oxide interested, R¹It is the alkyl of about 8 to about 24 carbon atoms；R²And R³It is the alkyl or hydroxyalkyl or its mixture of 1-3 carbon atom；R²And R³For example it can be connected to by oxygen or nitrogen-atoms on each other, to form ring structure；R⁴It is alkylidene or hydroxy alkylidene containing 2-3 carbon atom；N scope is 0 to about 20.

Useful water-soluble amine oxides surfactant is selected from cocounut oil or tallow alkyl two (low alkyl group) amine oxide, its instantiation is DDAO, tridecyl dimethyl amine, CH3-(CH2)13N(CH3)2-O, tridecyldimethylamine base amine oxide, cetyl dimethyl amine oxide, pentadecyldimethylamine base amine oxide, octadecyl dimethyl amine oxide, heptadecyldimethylamine oxide, octadecyldimethylamine oxide, cetyl dipropyl amine oxide, myristyl dibutyl amine oxide, octadecyl dibutyl amine oxide, double (2- ethoxys) dodecyl amine oxides, double (2- ethoxys) -3- dodecyls epoxide (dodecoxy) -1- hydroxypropylamine oxides, dimethyl-(2- hydroxydodecyls) amine oxide, 3, 6, (octadecyl) the dimethyl amine of 9- tri- and (2- ethoxys) amine oxide of 3- dodecyl epoxide 2- hydroxypropyls two.

Useful semi-polar nonionic surfactants also include the water soluble oxidized phosphine with following structures：

Wherein arrow is the conventional expressing method of semi-polar bond；R¹It is alkyl, alkenyl or hydroxyalkyl moiety that chain length range is 10 to about 24 carbon atoms；R²And R³Respectively independently selected from the alkyl containing 1-3 carbon atom or the moieties of hydroxyalkyl.

The example of useful phosphine oxide includes dimethyldecylphosphine oxide, dimethyl tetradecyl base phosphine oxide, Methylethyl tetradecylphosphine oxide, dimethyl hexadecyl base phosphine oxide, diethyl -2- hydroxyl octyl-decyls phosphine oxide, double (2- ethoxys) dodecylphosphine oxides and double (methylol) tetradecylphosphine oxides.Useful herein semi-polar nonionic surfactants also include the water soluble sulfoxide compounds with following structures：

Wherein arrow is the conventional expressing method of semi-polar bond；R¹It is alkyl or hydroxyalkyl moiety that carbon number is about 8 to about 28,0-5 ehter bond and 0 to about 2 hydroxyl substituent；R²The moieties being made up of alkyl and hydroxyalkyl with 1-3 carbon atom.

The useful example of these sulfoxides includes dodecyl methyl sulfoxide；3- hydroxy tridecyl methyl sulfoxides；3- methoxytridecylmethysulfoxide sulfoxides；With 3- hydroxyl -4- dodecyl epoxide butyl methyl sulfoxides.

Preferred semi-polar nonionic surfactants for the present composition include dimethyl amine, such as lauryl dimethyl amine oxide, myristyl dimethyl amine oxide, cetyl dimethyl amine oxide, its combination and analog.

Silicone surfactant

Silicone surfactant may include modified diakyl-polysiloxane, such as dimethyl polysiloxane.One or more hydrophilic polyalkylene oxide base modified polyorganosiloxane hydrophobic groupings can be used.This surfactant can provide low surface tension, it is high soak, high spreadability, froth breaking and excellent stain removal.The silicone surfactant of the present invention includes being grafted to polydialkysiloxane thereon, such as dimethyl silicone polymer by hydrosilanes glycosylation reaction polyethers, typically polyalkylene oxide base.This method causes the copolymer of alkyl pendant (AP types), and wherein polyalkylene oxide base is connected by a series of Si-C keys of hydrolysis-stables along siloxane main chain.

The polydialkysiloxane product of these nonionics substitution has following formulas：

Wherein PE represents non-ionic group, such as-CH₂-(CH₂)_p-(EO)_m(PO)_n- Z, wherein EO represent oxirane, and PO represents expoxy propane, x is the numeral that scope is about 0 to about 100, and y is the numeral that scope is about 1-100, and m, n and p are the numerals that scope is about 0 to about 50, m+n >=1, and Z represent hydrogen or R, and each of which R independently represents rudimentary (C_1-6) straight or branched alkyl.Molecular weight (the M of this surfactant_n) it is about 500-20,000.

Other silicone nonionic surfactants have following formula：

PA=-(C₂H₄O)_a(C₃H₆O)_bR or

Wherein x represents numeral of the scope as about 0 to about 100, and y represents numeral of the scope as about 1 to about 100, and a and b represent the numeral that scope independently is about 0 to about 60, and a+b >=1 and every R independently are H or lower straight or side chain (C_1-6) alkyl.Second group of nonionic siloxane surfactant is alkoxy end-capped (AEB types), and it is not so preferably as Si-O- keys provide limited hydrolytic resistance, but the fast decoupled in sour environment under conditions of neutral or slightly alkaline.Suitable surfactant is with trade name

Trade name

Or with trade name

Sale.A kind of useful surfactant

L77 has following formula：

(CH₃)₃Si-O(CH₃)Si(R¹)O-Si(CH₃)₃

Wherein R¹=-CH₂CH₂CH₂-O-[CH₂CH₂O]_zCH₃；Wherein z is 4-16, most preferably preferably 4-12,7-9.

Other useful surfactants include TEGOPREN

ABIL

ABIL And ABIL

Anion surfactant

The surface reactive material for being categorized into anion surfactant of the present invention is equally applicable to, because the electric charge on hydrophobe is negative；Or wherein the hydrophobic part of molecule does not carry the surfactant (for example, carboxylic acid) of electric charge (unless rise pH to neutral or higher).Carboxylate radical, sulfonate radical, sulfate radical and phosphate radical are polarity (hydrophilic) solubilizing groups found in anion surfactant.Among the cation (counter ion counterionsl gegenions) combined with these polar groups, sodium, lithium and potassium assign solubility in water；Ammonium and the ammonium ion of substitution provide the solubility in water and oil；Calcium, barium and magnesium promote the solubility in oil.

Anion surfactant is excellent detersive surfactant, therefore is advantageously added in heavy duty detergent composition.However, in general, anion surfactant has high foam property, this property limits it and is used alone or is used in higher concentrations in cleaning system, such as CIP circuits for requiring strict foam control.In addition, anionic surfactant compound can assign the special chemical or physical property in addition to decontamination in said composition.Anion surfactant can be used as gelling agent or the gelling as part or thickened systems.Anion surfactant is excellent solubilizer and controlled available for hydrotropic effect and cloud point.

Most of in many commercial anionic surfactants can be subdivided into five main chemical groups and extra subgroup, these " Surfactant Encyclopedia ",Cosmetics& Toiletries,It is disclosed in Vol.104 (2) 71-86 (1989).First group includes acylamino acid (and salt); such as acyl group gluamate, acyl group peptide, sarcosinate are (for example; N- acyl sarcosinates), tartrate (for example, fatty acid amide of N- acyl group tartaric acids salt and methyl tauride) and analog.Second group includes carboxylic acid (and salt), such as alkanoic acid (and alkanoate), ester carboxylic acid (for example, butanedioic acid alkyl ester), ether carboxylic acid and analog.3rd group includes phosphate and its salt.4th group includes sulfonic acid (and salt), such as isethionate (such as acyl-hydroxyethyl sulfonate), alkylaryl sulfonates, alkylsulfonate, sulfosuccinate (monoesters and diester of such as sulfosuccinic acid) and analog.5th group includes sulfuric ester (and salt), such as alkyl ether sulfate, alkyl sulfate and analog.Although can the present composition using these organize anion surfactant in each, it should be noted that, some in these anion surfactants may be incompatible with enzyme.For example, acylamino acid and salt may be incompatible with protease, because caused by their peptide structure.

The anionic sulphate surfactant for being suitable for using in the compositions of the present invention includes the primary and secondary alkyl sulfate of straight chain and side chain, alkyl ethoxy sulfate, fatty oleyl glycerol sulfates, alkylphenol ethylene oxide ether sulfates, C₅-C₁₇Acyl group-N- (C₁-C₄Alkyl) and-N- (C₁-C₂Hydroxyalkyl) glucosamine sulfate, and alkyl polysaccharide sulfate, the sulfate (nonionic non sulphate compound described herein) of such as alkyl poly glucoside.

Suitable synthesis, the example of water soluble anionic detergent compound include the ammonium and substitution ammonium of alkyl mononuclear aromatic sulfonic acid (such as the alkyl in straight or branched contains the alkyl benzene sulphonate of about 5 to about 18 carbon atoms) (for example, it is single-, two/and triethanolamine) and alkali metal (such as sodium, lithium and potassium) salt, such as alkylbenzenesulfonate, or alkyl toluene, dimethylbenzene, the salt of cumene and phenolsulfonic acid；Alkylnaphthalene sulfonate, diamyl machine naphthalene sulfonate, and dinonylnaphthalene sulfonic acid salt and oxidation alkyl derivative.

The anionic carboxylic acid salt surfactant for being suitable for using in the compositions of the present invention includes alkyl ethoxy carboxylate, alkyl polyethoxy multi-carboxylate's surfactant, and soaps (such as alkyl carboxyl).Secondary type soaps surfactant (for example, alkyl carboxyl surfactants) available for the present composition includes those containing the carboxyl units being connected in secondary carbon.Secondary carbon can be in ring structure, such as in the ring structure to octyl benzoic acid or alkyl-substituted cyclohexane carboxylic acid salt.Secondary type soaps surfactant typically not ether-containing key, ester bond or hydroxyl.In addition, they typically lack nitrogen-atoms in headgroup (both sexes part).Suitable secondary type soaps surfactant is typically containing 11-13 total carbon atoms, but more carbon atoms may be present (for example, most 16).

The other anionic detergents for being suitable for using in the compositions of the present invention include the mixture of alkene sulfonate, such as long-chain olefin sulfonate, long-chain hydroxyl alkane sulfonate, or alkene sulfonates and hydroxyl alkane sulfonate.Equally include alkyl sulfate, poly- (ethyleneoxy group) ether sulfate of alkyl and poly- (ethyleneoxy group) sulfate of aromatics, the sulfate or condensation product of such as oxirane and nonyl phenol (usual each molecule has 1-6 epoxide ethylidene).Resin acid and hydrogenated resin acid are also suitable, for example, be present in or derived from the rosin in tallow oil, hydrogenated rosin and resin acid and hydrogenated resin acid.

The need for depending on specific formula and in it, suitably selected specific salt.

" the further example of suitable anion surfactant is being given in Surface Active Agents and Detergents " (Vol I and II, Schwartz, Perry and Berch).Various this surfactants are equally generally disclosed in the row of the 58th the-the 29 column of row of the 23rd column the 23rd for authorizing Laughlin et al. United States Patent (USP) No.3929678 on December 30th, 1975.

In one embodiment, composition of the invention includes alkyl or alkylaryl sulfonates or substituted vitriol salt and sulfated product.In some embodiments, composition of the invention includes branched paraffin sulfonate, branch-alkylbenzene sulfonate, alpha-alkene sulfonate, alkyl sulfate, secondary alkane sulfates or sulfonate, or sulfosuccinate.

Cationic surfactant

If the electric charge on the hydrotropic agent part of surfactant molecules is just, the surface reactive material is categorized as cation.Be also included in this group be the wherein cation of hydrotropic agent neutral (except non-reduced pH is to close to neutral or lower) surfactant (such as alkanamine).In theory, can be by containing " " structure R_nX⁺Y^-Any combination of element carry out synthesizing cationic surfactant, and it may include the compound in addition to nitrogen (ammonium), such as phosphorus (Phosphonium) and sulphur (sulfonium) compound.In practice, cationic surfactant field is leading by nitrogen-containing compound, it may be possible to which, because the synthetic route of cationic nitrogenous surfactant is simple and directly and obtains the high yield of product, this make it that they are less expensive.

Cationic surfactant is preferably included, and more preferably refers to the nitrogen compound of hydrophobic grouping and at least one positively charged containing at least one Long carbon chain.Long carbon chain group can be directly connected on nitrogen-atoms by simply replacing, or is more preferably connected indirectly to by one or more bridging functional groups in so-called alkylamines and amido amine on nitrogen-atoms.This functional group may be such that the molecule is more hydrophilic and/or more adds water dispersible, it is easier to by co-surfactant blend in water solubilized, and/or water soluble.In order to increase solubility in water, extra primary, secondary or tertiary amino, or the quaternary ammonia nitrogen of available low molecular weight alkyl can be introduced.In addition, nitrogen can be the part in the side chain or linear fraction of various degrees of unsaturation or saturation or unsaturated heterocycle.In addition, cationic surfactant can contain with the complex bonds for being more than a cationic nitrogen atom.

The surfactant compounds of amine oxide, both sexes and amphiphilic surfactant are categorized as itself to be typically cation in weakly acidic pH to acidic pH solutions and can intersect the classification of surfactant.The behavior of oxyethylated cationic surfactant is usual as nonionic surfactant in alkaline solution, and in an acidic solution as cationic surfactant.

Therefore simplest cationic amine, amine salt and quaternary ammonium compound can be illustrated：

Wherein R represents chain alkyl, and R, R " and R " ' can be long alkyl chain or less alkyl or aryl or hydrogen, X represents anion.Amine salt and quaternary ammonium compound are useful due to its high water solubility.

Most of in many commercial anionic surfactants can be subdivided into four groups and known to those skilled in the art or known extra subgroup, as at " SurfactantEncyclopedia ", described in Cosmetics ＆ Toiletries, Vol.104 (2) 86-96 (1989).First group includes alkanamine and its salt.Second group includes alkyl imidazoline.3rd group includes ethoxylated amine.4th group includes quaternaries, such as alkyl benzyl dimethyl ammonium salt, alkylbenzene salt, heterocyclic ammonium salts, tetraalkylammonium salt and analog.Known cationic surfactant has various performances beneficial in the compositions of the present invention.Performance needed for these may include the detergency for being less than composition under neutral pH, antimicrobial efficiency, the thickening cooperated with other reagents or gelling property, and similar performance.

Cationic surfactant available for the present composition includes having chemical formula R¹ _mR² _xY_LThose of Z, each of which R¹It is the organic group containing straight or branched alkyl or alkenyl, the organic group is optionally replaced with most three phenyl or hydroxyl and optionally separated with the isomers or mixture of most 4 following structures or these structures, and the organic group contains about 8-22 carbon atom：

R¹Base can additionally contain most 12 ethyoxyls, and m is 1-3 numeral.Preferably, when m is 2, no more than one R of intramolecular¹With 16 or more carbon atom, or when m is 3, with more than 12 carbon atoms.Every R²It is alkyl or hydroxyalkyl or benzyl containing 1-4 carbon atom, and intramolecular is no more than a R²It is benzyl, and x is 0-11 numeral, preferably 0-6.Remaining any carbon atom position on Y bases is filled with hydrogen.

Y can be including but not limited to following groups,

P=about l to 12

P=about l to 12

Or its mixture.Preferably, L is 1 or 2, and when L is 2, Y bases are selected from the R with 1 to about 22 carbon atom and two free carbon single bonds¹And R²Homologue (preferably alkylidene or alkenylene) part separate.Z is water soluble anion, for example halogen ion, sulfate radical, methylsulfate, hydroxyl or nitrate anion, particularly preferred chlorion, bromide ion, iodide ion, sulfate radical or methyl sulfate anions, and its quantity causes the cationic componentses for obtaining electroneutral.

Amphoteric surfactant

Amphoteric surfactant is simultaneously containing alkalescence and acidic hydrophilic groups and organic hydrophobic grouping.These ionic entities can be herein for any anion or cation group described in other types of surfactant.Basic nitrogen and acid carboxylic acid group are used as the typical functional group of alkalescence and acidic hydrophilic groups.In some surfactants, sulfonate radical, sulfate radical, phosphonate radical or phosphate radical provide negative electrical charge.

Amphoteric surfactant can be broadly described as the derivative of aliphatic secondary and tertiary aminess; wherein aliphatic group can be straight or branched; and wherein one of aliphatic series substituent contains about 8-18 carbon atom; contain anionic water solubilizing group with one, such as carboxyl, sulfo group, sulfato, phosphate radical are closed or phosphoryl.Amphoteric surfactant is subdivided into two main groups well known by persons skilled in the art, and be disclosed in " Surfactant Encyclopedia ",Cosmetics & Toiletries, in Vol.104 (2) 69-71 (1989).First group includes acyl group/Dialkylaminoethylamine derivative (for example, 2- alkyl hydroxyethyl imidazolines derivative) and its salt.Second group includes N- alkyl amino acids and its salt.Think that some amphoteric surfactantes may conform to this two class.

Amphoteric surfactant can be synthesized by method known to those skilled in the art.For example, by being condensed long-chain carboxylic acid's (or derivative) and Dialkylaminoethylamine and closed loop, so as to synthesize 2- alkyl hydroxyethyl imidazolines.By then hydrolyzing and by alkylation, such as, with chloracetic acid or ethyl acetate, making imidazoline ring open loop, carry out the amphoteric surfactant of derivatization business.In alkylation process, one or two carboxyalkyl and different alkylation reactions form tertiary amine and ehter bond, so as to obtain different tertiary amines.

Available long-chain imdazole derivatives generally have following formulas in the present invention：

(list) acetate (two) propionate both sexes sulfonate

Neutral pH-amphion

Wherein R is the acyclic hydrophobic group containing about 8-18 carbon atom, and M is the cation for neutralizing anionic charge, typically sodium.Amphoteric surfactant derived from the commercial promising imidazoline that can be used in the compositions of the present invention is included for example：N- (cocoamidoethyl)-N- (2- hydroxyethyls) alanine sodium, N- (cocoamidoethyl)-N- (2- ethoxy-cs acidic group) alanine sodium, N- (cocoamidoethyl)-N- (2- hydroxyethyls) Sodium Glycinate, N- cocoamidoethyl-N- ethoxy-N- carboxymethylamino sodium acetates, N- (cocoamidoethyl)-N- (2- Hydroxy-ethylaminos) -2- hydroxypropyl sulfonates and N- (cocoamidoethyl)-N- (2- ethoxy-cs acidic group) alanine.By the dicarboxyl acid functionality in wherein both sexes dicarboxylic acids preferred both sexes carboxylic acid is produced for the fatty imidazolines of oxalic acid and/or dipropionic acid.

The carboxymethylated compounds (glycinate) often described above are referred to as glycine betaine.Glycine betaine is the one group of special amphoteric surfactant discussed in entitled following amphiphilic surfactant part.

Easily pass through RNH₂(wherein R=C₈-C₁₈Straight or branched alkyl), fatty amine and halogenated carboxylic acids react, so as to prepare long-chain N- alkyl amino acids.The alkylation of primary amino radical causes secondary and tertiary aminess in amino acid.Alkyl substituent can have extra amino, and the extra amino, which is provided, is more than a reactive nitrogen center.The N- alkyl amino acids of most of business are the alkyl derivatives of Beta-alanine or β-N (2- carboxyethyls) alanine.The example of the business N- alkyl amino acid ampholytes used in the present invention includes beta-amino dipropyl acid alkyl ester, RN (C₂H₄COOM)₂And RNHC₂H₄COOM.In these, R is preferably the acyclic hydrophobic group containing about 8 to about 18 carbon atoms, and M is the cation for neutralizing anionic charge.

It is preferred that amphoteric surfactant include derived from coconut products those, such as coconut oil or coco-nut oil fatty acid.The surfactant of these preferred coconut deriveds includes ethylenediamine moiety, alkanolamide moiety, amino acid moiety, preferably glycine, or it is combined as its part-structure；The aliphatic substituent of about 8-18 (preferably 12) individual carbon atom.This surfactant can also be considered as alkyl both sexes dicarboxylic acids.Cocounut oil both sexes disodium beclomethasone is a kind of most preferred amphoteric surfactant, and can be with trade name Miranol^TMFBS is available commercially from RhodiaInc., Cranbury, N.J..The amphoteric surfactant of another most preferably coconut derived with chemical name cocounut oil both sexes diethyl acid disodium is with trade name Miranol^TMC2M-SF Conc. sell (being obtained also from Rhodia Inc., Cranbury, N.J.).

The typical case that amphoteric type is given in the United States Patent (USP) No.3929678 that on December 30th, 1975 authorizes Laughlin and Heuring enumerates material with these surfactants." giving further example in Surface Active Agents and Detergents " (Vol.I and II, S chwartz, Per ry and Berch).

Amphiphilic surfactant

Amphiphilic surfactant can be considered as the subgroup of amphoteric surfactant.Amphiphilic surfactant can be broadly described as the derivative of the derivative of secondary and tertiary aminess, the derivative of heterocycle secondary and tertiary aminess, or quaternary ammonium, quaternary phosphines or tertiary sulfonium compound.Typically, amphiphilic surfactant includes the quaternary ammonium of positively charged, or in some cases, Liu Huo Phosphonium ions；Electronegative carboxyl；And alkyl.Amphiphilic surfactant usually contains cation and anionic group, and the cation and anionic group can form strong " inner salt " attraction in the into almost equal degree of the isoelectric zone internal ionization of the molecule between positive-negative charge-site.The example of this amphiphilic synthetic surfactant includes the derivative of aliphatic quaternary ammonium, Phosphonium and sulfonium compound, aliphatic group therein can be straight or branched, one of wherein aliphatic substituent contains the water solubilizing group of 8-18 carbon atom and one containing anion, such as carboxyl, sulfonate radical, sulfate radical, phosphate radical or phosphonate radical.Glycine betaine and sulfobetaine surfactant are the amphiphilic surfactants used herein enumerated.

The formula of these compounds is：

Wherein R¹Alkyl, alkenyl or hydroxyalkyl containing the 8-18 carbon atom with 0-10 ethylene oxide moiety and 0-1 glyceryl moiety；Y is selected from nitrogen, p and ses atom；R²It is alkyl or monohydroxy alkyl containing 1-3 carbon atom；When Y is sulphur atom, x is 1, and when Y is nitrogen or phosphorus atoms, x is 2, R³It is the alkylidene or hydroxy alkylidene or hydroxy alkylidene of 1-4 carbon atom, and Z is the group selected from carboxylate radical, sulfonate radical, sulfate radical, phosphonate radical and phosphate groups.

The example of amphiphilic surfactant with structure listed above includes：4- [(2- the ethoxys)-N- octadecyls of N, N- bis- ammonium] butane -1- carboxylates, 5- [S-3- hydroxypropyl-S- cetyl sulfoniums base] -3- hydroxyl pentane -1- sulfate；3- [P, P- diethyl-P-3, the Nian Wan Phosphonium base of 6,9- trioxa four] 2- hydroxy propane -1- phosphate；3- [N, N- dipropyl-N-3- dodecyloxy -2- hydroxypropyls ammonium]-propane -1- phosphonates；3- (N, N- dimethyl-N-hexadecyl base ammonium)-propane-sulfonate；3- (N, N- dimethyl-N-hexadecyl base ammonium) -2- hydroxy-propane -1- sulfonate；4- [N, N- bis- (2 (2- ethoxys)-N (2- hydroxydodecyls) ammonium)-butane -1- carboxylates；3- [S- ethyls-S- (3- dodecyloxy -2- hydroxypropyls) sulfonium base] propane -1- phosphate；3- [P, P- dimethyl-P- Shi bis- Wan Ji Phosphonium base]-propane -1- phosphonates；With S [(3- the hydroxypropyls)-N- cetyls of N, N- bis- ammonium] -2- hydroxyls-pentane -1- sulfate.Alkyl in the detergent surfactant can be straight or branched and saturation or unsaturated alkyl.

The amphiphilic surfactant for being suitable for using in the compositions of the present invention includes the glycine betaine of following formulas：

These surfactant betaines do not show strong cation or anionic character typically under extreme pH, and they do not show reduced water solubility in the electric scope of its grade yet.From unlike " outer " quaternary ammonium salt, glycine betaine is compatible with anion surfactant.The example of suitable glycine betaine includes acylamidopropyldimethyl glycine betaine；Cetyl dimethyl betaine；C_12-14Amido propyl betaine；C_8-14Acylamidohexyldiethyl glycine betaine；4-C_14-16Sulfonylmethyl amide groups diethyl ammonium -1- carboxybutanes；C_16-18Acylamidodimethylbetaine glycine betaine；C_12-16Amide groups pentane diethyl betaines；And C_12-16Acylmethylamidodimethylbetaine glycine betaine.

Sulfobetaines available for the present invention includes having chemical formula (R (R¹)₂N⁺R²SO^3-) those compounds, wherein R is C₆-C₁₈Alkyl, every R¹Typically stand alone as C₁-C₃Alkyl, such as methyl, and R²It is C₁-C₆Alkyl, such as C₁-C₃Alkylidene or hydroxy alkylidene.

The typical case that amphiphilic type is given in the United States Patent (USP) No.3929678 that on December 30th, 1975 authorizes Laughlin and Heuring enumerates material with these surfactants." giving further example in Surface Active Agents and Detergents " (Vol.I and II, Schwartz, Perry and Berch).

Surface activator composition

In the practice and purposes of the present invention, above-described surfactant can be used alone or in combination.Especially, nonionic surfactant and anion surfactant may be used in combination.Semi-polar nonionic, cation, both sexes and amphiphilic surfactant can combine nonionic or anion surfactant is used together.Examples detailed above is only that can find that the specific of available many surfactants is enumerated within the scope of the invention.Foregoing organic surfactant compounds can be configured to any one of several commercial required composition forms of the present invention with disclosed purposes.The composition includes the carrying out washing treatment agent for contaminated surface of conc forms, when the carrying out washing treatment agent dilutes suitably by proportioning device, when being dispersed or dissolved in water, and being transported in the form of a solution on target surface, gel or foam will provide cleaning.The cleaning treatment agent is made up of a kind of product, or including wherein using two kinds of product systems of each ratio.The product is typically liquid or the concentrate of emulsion.

Hydrotropic agent

Hydrotropic agent is usually used in formula, to maintain pure single-phase or Aquo-composition.This reagent can also be used in the present invention.The hydrotropy is to be related to solubility or the performance of miscibility that material improvement wherein material tends to the material in insoluble liquid phase in it.The material for providing the hydrotropy is referred to as hydrotropic agent, and compared with treating the material of solubilising, can be used with relatively low concentration.Hydrotropic agent improved formulations, as a result increase the solubility of insoluble matter or produce micella or mixed micelle structure, so as to cause the stable suspension of insoluble matter.In the present invention, hydrotropic agent most usefully maintains preparation process and neutralized when the uniform solution of each component in the formula when using area is scattered.Single phase soln that hydrotrope solubilizers can maintain each component to be evenly distributed in the composition is in aqueous or non-aqueous form.

It is preferred that hydrotrope solubilizers used with about 0.1 to about 30wt% concentration, and including such as small molecule anionic surfactant and semi-polar nonionic surfactant.The most preferred scope of hydrotrope solubilizers is about 1 to about 20wt%.Relatively well known to be, Hydrotrope materials show hydrotropic performance in the chemical molecule types of wide scope.Hydrotropic agent generally includes ether compound, alcoholic compound, anion surfactant, cationic surfactant and other materials.A kind of important hydrotrope solubilizers that the present invention is used include amine oxide material.Small molecule anionic surfactant includes aromatic sulfonic acid or sulfonate hydrotropes, such as C_1-5Substituted benzene sulfonic acid or naphthalene sulfonic acids.This hydrotropic example is xylene monosulfonic acid or naphthalene sulfonic acids or its salt.

The nonionic surfactant of semi-polar type includes amine oxide hydrotropes, such as tertiary amine oxide corresponding to following formulas：

Wherein n is 0-25, and arrow is the conventional expressing method of semi-polar bond；R₁、R₂And R₃Can be aliphatic series, aromatics, heterocycle, alicyclic or its combination.For detergent applications amine oxide interested, R₁It is about 8 to about 24 side chains of carbon atom or the aliphatic series of straight chain or alkyl；R₂And R₃Alkyl or hydroxyalkyl or its mixture selected from 1-3 carbon atom；R₄It is alkylidene or hydroxy alkylidene containing 2-3 carbon atom；N scope is 0 to about 20.Useful water-soluble amine oxides hydrotropic agent is selected from alkyl two (low alkyl group) amine oxide, and its instantiation is C_10-14Amine base amine oxide, (Permethyl 99A base) dimethyl amine-Barlox12i, positive decyl dimethyl amine, DDAO, tridecyl dimethyl amine, CH3-(CH2)13N(CH3)2-O, tridecyldimethylamine base amine oxide, cetyl dimethyl amine oxide, pentadecyldimethylamine base amine oxide, octadecyl dimethyl amine oxide, heptadecyldimethylamine oxide, octadecyldimethylamine oxide, cetyl dipropyl amine oxide, myristyl dibutyl amine oxide, octadecyl dibutyl amine oxide, double (2- ethoxys) dodecyl amine oxides, double (2- ethoxys) -3- dodecyloxies (dodecoxy) -1- hydroxypropylamine oxides, dimethyl-(2- hydroxydodecyls) amine oxide and 3, 6, more than 9- tri- (octadecyl) dimethyl amine it is most preferably Permethyl 99A base dimethyl amine (Barlox12i).Other hydrotropic agents or coupling agent (coupler) can be generally used in the compositions of the present invention, to maintain the integrality and storage stability of physical single phase.Therefore, any amount of composition known to the those of ordinary skill maying be used in formulation art, such as simple function and polyfunctional alcohol.These preferably comprise from about 1 to about 6 carbon atom and 1 to about 6 hydroxyl.Example includes ethanol, isopropanol, normal propyl alcohol, 1,2-PD, 1,2- butanediols, 2- methyl -2,4- pentanediol, sweet dew alcohol and glucose.Equally available is higher glycols, polyalcohol, poly oxide, glycol ether and propylene glycol.Extra useful hydrotropic agent includes the alkali metal salt of free acid and sulfonation alkaryl, such as alkylated dipheny ethers sulfonate, toluene, dimethylbenzene, cumene and phenol or phenol ether sulfonates or alkoxylated diphenyl oxide disulfonate (Dowfax materials)；Alkyl and dialkyl naphthalene sulfonate salts and alkoxy derivative.Be not generally regarded as these hydrotropic sulfonate materials be strong surfactant types material.These materials are the sulfonate of the hydrophobic grouping with connection, and the sulfonate is designed to provide hydrotropic performance, rather than surfactant properties.It should keep firmly in mind, these materials are not generally regarded as being surface activator composition.

Sequestering agent

The Cleasing compositions of the present invention may include sequestering agent.In general, sequestering agent is to be coordinated and (that is, be bonded) molecule of the metal ion generally found in natural water, to prevent the effect of other decontamination compositions in metal ion disturbance Cleasing compositions.This chelating agent/sequestering agent when with effective dose including when also act as threshold agent (threshold agent).On the discussed further of chelating agent/sequestering agent, referring to, Kirk-Othmer,Encyclopedia of Chemical Technology, the 3rd edition, volume 5, the 339-366 pages and volume 23, the 319-320 pages.

Various sequestering agents can be used in the heterogeneous Cleasing compositions of the present invention, including such as organic phosphonate, amino carboxylic acid, condensed phosphate, inorganic builders, multi-carboxylate polymer, two or tricarboxylic acids, its mixture or the like.This sequestering agent and builder are commercially available.In some embodiments, heterogeneous Cleasing compositions of the invention include about 5 to about 50wt%, about 30 to about 50wt%, about 10 to about 45wt%, or about 20 to about 40wt% sequestering agent.In some embodiments, heterogeneous Cleasing compositions of the invention include about 20wt%, about 25wt%, about 30wt%, about 35wt% or about 40wt% sequestering agent.Said composition may include not with any one in these scopes or content about changed.

Suitable condensed phosphate includes sodium orthophosphate and potassium, sodium pyrophosphate and potassium, sodium tripolyphosphate and potassium, such as calgon, tripolyphosphate.In one embodiment, heterogeneous Cleasing compositions of the invention include condensed phosphate, and such as sodium tripolyphosphate is used as builder, chelating agent or sequestering agent.

Being adapted for use as the polycarboxylate of sequestering agent is included for example, polyacrylic acid, maleic acid/olefin copolymer, acrylic acid/maleic acid, polymethylacrylic acid, acrylic acid-methacrylic acid copolymer, the polyacrylamide of hydrolysis, the polymethacrylamide of hydrolysis, the polyamide-methacrylamide copolymer of hydrolysis, the polyacrylonitrile of hydrolysis, the polymethacrylonitrile of hydrolysis, acrylonitrile-methacrylonitrile copolymers of hydrolysis, poly, poly- fumaric acid, the copolymer of acrylic acid and itaconic acid, and analog, in one embodiment, polycarboxylate includes polyacrylate.

Suitable two or tricarboxylic acids include oxalic acid, citric acid or its salt.In one embodiment, oxalic acid can be used for reduction application composition in iron content or from product to be cleaned remove de-iron dirt.For example, oxalic acid can be a part for the acid either iron removing reagent for controlling iron.

In one embodiment, heterogeneous Cleasing compositions of the invention include condensed phosphate and polyacrylate or another polymer, and such as sodium tripolyphosphate and polyacrylate are used as sequestering agent or builder.

Builder may include organic phosphonate, such as organic phospho acid and its alkali metal salt.Some examples of suitable organic phosphonate include：

1- hydroxyl ethane -1,1- di 2 ethylhexyl phosphonic acids：CH₃C(OH)[PO(OH)₂]₂；

Amino three (methylene phosphonic acid)：N[CH₂PO(OH)₂]₃；

Amino three (methylene phosphonic acid) sodium salt：

2- hydroxyethyls imino group is double (methylene phosphonic acid)：

HOCH₂CH₂N[CH₂PO(OH)₂]₂；

Diethylene triamine penta(methylene phosphonic acid)：

(HO)₂POCH₂N[CH₂CH₂N[CH₂PO(OH)₂]₂]₂；

Diethylene triamine penta(methylene phosphonic acid) sodium salt：C₉H_(28-x)N₃Na_xO₁₅P₅(x=7)；Hexamethylene diamine (tetramethylene phosphonic acid) sylvite：C₁₀H_(28-x)N₂K_xO₁₂P₄(x=6)；Double (hexa-methylene) triamines (pentamethylene phosphonic acids)：(HO₂)POCH₂N[(CH₂)₆N[CH₂PO(OH)₂]₂]₂；With phosphoric acid H₃PO₃；With other similar organic phosphonates, and its mixture.

Sequestering agent can be or the sequestering agent including aminocarboxylic acid type.The sequestering agent of suitable aminocarboxylic acid type includes acid or its alkali metal salt, such as amion acetic acid and its salt.Some examples include following：

N- hydroxyethylaminodiacetic acids；

Hydroxyethylenediamine tetracetic acid, nitrilotriacetic acid (NTA)；

MDGA (MGDA)；

Ethylenediamine tetra-acetic acid (EDTA)；

N-hydroxyethyl-ethylenediamine triacetic acid (HEDTA)；

Diethylene-triamine pentaacetic acid (DTPA)；With

Alanine-N, N- oxalic acid；

Iminodisuccinic acid；

And analog；And its mixture.

A kind of useful builder/chelating agent or its salt include polymerization phosphinocarboxylic acid, including its salt and its derivative.Can be by making unsaturated carboxylic acid monomer such as acrylic acid and the usual hypophosphorous acid represented with following formula or derivatives thereof reaction, so as to prepare this material：

Wherein R₁It is group OX, wherein X is hydrogen or the straight or branched alkyl containing 1-4 carbon atom；And R₃It is the straight or branched alkyl of hydrogen or 1-8 carbon atom, the cycloalkyl of 5-12 carbon atom, phenyl, benzyl or-OX groups, wherein X is the straight or branched alkyl of hydrogen or 1-4 carbon atom.The salt of poly- phosphinocarboxylic acid can also be used as described above.One preferred embodiment of this material is Belsperse

-161。

Sequestering agent can be or including biodegradable sequestering agent.Suitable biodegradable sequestering agent includes MDGA or its salt.This sequestering agent for example with trade name Trilon ES commercially available from.

Enzyme

The Cleasing compositions of the present invention may include one or more enzymes, and the enzyme can provide required activity to remove protein-based, carbohydrate-based or triglycerides base dirt from substrate for cleaning, decontamination and pre-soaking.Although not limiting the present invention, but being suitable for the enzyme of the Cleasing compositions of the present invention can be worked by the soil residues for one or more types that degraded or change run on surface or textile, so that by the way that other components in surfactant or Cleasing compositions are except crude removal or dirt is more removed.Can be by reducing the physical-chemistry force that dirt is attached on surface to be cleaned or textile, i.e. make dirt more water soluble, by not only degrading but also changing soil residues, to improve soil release performance.For example, one or more protease can make to be present in complex compound in soil residues, macromolecular protein structures body and be dissociated into better simply short chain molecule by the decontaminating solution containing the protease, the short chain molecule is more prone to desorb from surface in itself, otherwise dissolve or be more prone to remove.

Suitable enzyme includes any suitable source, such as protease of plant, animal, bacterium, fungi or yeast sources, amylase, lipase, glucose enzyme, cellulase, peroxidase or its mixture.It is preferred that selection influenceed by factors such as pH activity and/or optimizing stability, heat endurance and stability on activated detergent, builder and analog.On this point, preferred bacterium or fungal enzyme, such as bacterial amylase and protease, and fungal cellulase.Preferred enzyme is protease, lipase, amylase or its combination.

" detergency enzymes " as used herein otherwise refer to cleaning, decontamination or enzyme with beneficial effect as the component in composition to do washing, textile, dish washing, original place cleaning, drainpipe, ground, carpet, medical treatment or dental instruments, meat cutting tools, hard surface, personal nursing or the like.Suitable detergency enzymes include hydrolase such as protease, amylase, lipase or its combination.

Generally enzyme is incorporated into the present composition with the effective dose effectively cleaned in washing or presoaking procedure.Effective dose for cleaning refers to produce material to be cleaned cleaning, health, and the preferably consumption of corrosion-free outward appearance.Effective dose for cleaning can also refer to produced in substrate cleaning, except spot, decontaminate, brighten, the consumption of taste removal or freshness improving effect.Typically, about the 0.1% to about 3wt% of Cleasing compositions can be adopted as, preferably from about 1% to about 3wt% consumptions enzyme realizes this cleaning effect.Higher live vol is also preferable in highly concentrated cleaning formula.

Commercial enzyme can be obtained with liquid or dried forms, such as alkali protease, and select, purify with the thick aqueous solution or with classification, handling and compounded forms sale, and about 2% to the about 80wt% organized enzyme including generally being combined with stabilizer, buffer solution, confactor (cofactor), impurity and inert carrier.Actual active enzyme content depends on preparation method and is not crucial, if said composition has required enzymatic activity.To use the specific enzyme that selects to depend on final use condition in the method for the present invention and product, physical form including product, using pH, temperature in use and the soil type that digests, degrade or change is waited.For the use condition that any group of gives, enzyme may be selected to provide optimal activity and stability.

The composition of the present invention preferably includes at least one protease.Astoundingly it has furthermore been found that the composition of the present invention, which not only stablizes protease, reaches the shelf life significantly extended, and significantly improve the activity of the protease of digesting protein and improve stain removal.In addition, in the presence of one or more extra enzymes, such as amylase, cellulase, lipase, peroxidase, endoglucanase and its mixture, preferably lipase or amylase, there is the proteinase activity improved.

It can be targetted according to Cleasing compositions or select enzyme in soil type present on position to be cleaned or surface.Although not limiting the present invention, think that amylase is advantageously used for the dirt that cleaning contains starch, such as potato, pasta, oatmeal, baby food, gravy, chocolate or the like.Although not limiting the present invention, think that protease is advantageously used for the dirt that cleaning contains protein, such as scurf of blood, skin, mucus, grass, food (such as egg, milk, spinach, meat slag, tomato juice) or the like.Although not limiting the present invention, think that lipase is advantageously used for dirt of the cleaning containing fat, oil or wax, such as animal or plant fat, oil or wax (such as salad flavouring, butter, lard, chocolate, lipstick).Although not limiting the present invention, think that cellulase is advantageously used for dirt of the cleaning containing cellulose or the dirt containing cellulose fibre (it plays a part of the attachment point of other dirts).

Important references document on enzyme be " Industrial Enzymes " Scott, D.,Kirk-Othmer Encyclopedia of Chemical Technology, the 3rd edition (editor Grayson, M. and EcKroth, D.) volume 9, the 173-224 pages, John Wiley＆Sons, New York, 1980.

Protease

Plant, animal or microorganism can be derived from by being suitable for the protease of the present composition.Preferably, protease is derived from microorganism, such as yeast, mould or bacterium.It is preferred that protease include active serine protease at basic ph, it is preferably derived from the bacterial strain of bacillus such as bacillus subtilis or bacillus licheniformis；These preferred protease include natural and restructuring subtilisin.Protease can be purified or can be the component of microorganism extracts, and or be agriotype or variant (chemistry or the variant of restructuring).It is preferred that protease both do not suppressed by metal-chelator (sequestering agent) or thiol poison, also do not activated by metal ion or reducing agent, with wide substrate specificity, suppressed by diisopropylfluorophosphate (DFP), it is a kind of endopeptidase, molecular weight ranges are about 20,000 to about 40,000, and pH about 6 to about 12 times and temperature range be at about 20 DEG C to about 80 DEG C it is active.

The example for the protease that can be used in the compositions of the present invention includes (using trade name) Savinase

, protease derived from slow bacillus (Bacillus lentus) type, such as Maxacal

、Opticlean

、Durazym

And Properase

；With the protease derived from bacillus licheniformis, such as Alcalase

And Maxatase

；With the protease derived from bacillus amyloliquefaciens, such as Primase

.It is preferred that commercially available protease include with trade name Alcalase

、Savinase

、Primase

、Durazym

Or Esperase

Those sold by NovoIndustries A/S (Denmark)；With trade name Maxatase

、Maxacal

Or Maxapem

Those sold by Gist-Brocades (Holland)；With trade name Purafect

, Purafect OX and Properase sold by GenencorInternational those；With trade name Opticlean

Or Optimase

Those sold by Solvay Enzymes；And analog.The mixture of this protease can also be used.For example, Purafect

It is a kind of preferred alkali protease (subtilisin) used in the detergent composition of the present invention, it can be used for relatively low cleaning temp program, e.g., from about 30 DEG C to about 65 DEG C；And Esperase

The alkali protease for being a kind of higher temperature decontaminating solution for being used for about 50 DEG C to about 85 DEG C and selecting.Suitable decontamination protease is disclosed in following patent publications：Novo GB1243784, WO9203529A (enzyme/inhibitor system), WO9318140A and WO9425583 (trypsin-like protease of restructuring)；Procter＆Gamble WO9510591A, WO9507791A (there is the absorption of reduction and increased water-disintegrable protease), WO95/30010, WO95/30011, WO95/29979；GenencorInternational WO95/10615 (subtilisins of bacillus amyloliquefaciens)；EP130756A (protease A)；EP303761 (Cathepsin B)；And EP130756A.Misfolded proteins enzyme preferably at least 80% used in the present composition is homologous, preferably the amino acid sequence of the protease with least 80% sequence identity and in these references.

In a preferred embodiment of the invention, the amount ranges for being present in the commercial alkaline protease in the present composition are the about 0.1wt% to about 3wt% of decontaminating solution, preferably from about 1% to about 3wt%, preferably from about 2wt% commercial enzyme product solution.Typical commercially available detergency enzymes include about 5-10% organized enzyme.

Although the percetage by weight for determining required commercial alkaline protease be used for present invention teach that preparation embodiment be convenient in practice, but the change of commercial protease concentrate and environment additive on the spot and the negative effect to proteinase activity may require the more accurate analytical technology for proteasome assay, in the default activity for quantifying enzyme of preferred embodiment and to set up the correlation of the stability with polluting residue removing performance and enzyme；If if concentrate, then using dilute solution.The activity of the protease used in the present invention easily uses active unit, more specifically, is expressed with Kilo-Novo Protease Unit (KNPU) for unit, this is azocasein assay activity unit well known to the skilled person.Can be in entitled " The Use of Azoalbumin as a Substrate in t he ColorimetricDetermination of Peptic and Tryptic Activity " publication, Tomarelli, R.M., Charney, and Harding, M.L. J.J.Lab.Clin.Chem, the more detail discussion of azocasein assay procedure is found in 34,428 (1949).

In a preferred embodiment of the invention, it is about 1 × 10 to be present in using the field of activity of the protease in solution^-5KNPU/gm solution is to about 4 × 10^-3KNPU/gm solution.

Naturally, the mixture of different protease can be included in the present invention.Although the foregoing describing various specific enzymes, it is to be understood that can be used can assign composition needed for proteinase activity any protease, and do not limited anyway by specifically chosen protease the present invention this embodiment.

Amylase

Plant, animal or microorganism can be derived from by being suitable for the amylase of the present composition.Preferably, amylase is derived from microorganism, such as yeast, mould or bacterium.It is preferred that amylase include derived from bacillus, such as bacillus licheniformis, bacillus amyloliquefaciens, bacillus subtilis or bacillus stearothermophilus (B.stearothermophilus) those.Amylase can be purified or can be the component of microorganism extracts, and or be agriotype or variant (chemistry or the variant of restructuring), preferably variant more stable than the amylase of agriotype under the conditions of washing or pre-soaking.

The example for the amylase that can be used in the compositions of the present invention is included with trade name Rapidase by Gist-Brocades

Those of (Holland) sale；With trade name Termamyl

、Fungamyl

Or Duramyl

Those sold by Novo；With Purastar STL or the Purastar OXAM sold by Genencor；And analog.It is preferred that commercially available amylase include with trade name Duramyl

The stability-enhanced Variant Amylase sold by Novo.The mixture of amylase can also be used.

Being suitable for the amylase of the present composition includes：Alpha-amylase described in Novo WO95/26397, PCT/DK96/00056 and GB1296839；With in J.Biol.Chem., 260 (11)：6518-6521(1985)；In Novo WO9510603A, WO9509909A and WO9402957；Reference described in WO9402597；With the stability-enhanced amylase described in the WO9418314 in Genencor International.Variant alpha amylase used in the present composition can be homologous with least 80%, preferably the amino acid sequence of the protease with least 80% sequence identity and in these references.

With some amylase such as Termamyl

Compare, the suitable amylase available for the present composition has the stability improved.The stability of raising refer to respectively with suitable control starch enzyme such as Termamyl

Compared to when, one or more following aspects it is notable or measurable improvement：Oxidation stability, such as oxidation stability under pH9-10 in cushioning liquid to hydrogen peroxide/tetra-acetylated ethylene diamine；Heat endurance, such as heat endurance at common wash temperature such as from about 60 DEG C；And/or alkaline stability, such as alkaline stability under pH about 8 to about 11.Can be by measuring stability well known to a person skilled in the art method.Within the temperature range of 25 DEG C -55 DEG C and in the range of the pH of about 8 to about 10, the suitable stability-enhanced amylase available for the present composition has than Termamyl

Specific activity at least high 25% specific activity.Analysis method well known by persons skilled in the art and/or commercially available, such as Phadebas can be passed through

I- amylase assays, measurement amylase activity is for this comparison.

In one embodiment, the amount ranges for being present in the commercial amylases act in the present composition are the about 0.1wt% to about 3wt% of decontaminating solution, preferably from about 1% to about 3wt%, preferably from about 2wt% commercial enzyme product solution.Typical commercially available detergency enzymes include about 0.25-5% active amylase.

Although the percetage by weight for determining required amylase be used for present invention teach that preparation embodiment be convenient in practice, but the change of commercial amylases act concentrate and environment additive on the spot and the negative effect to amylase activity may require the more accurate analytical technology for amylase assay, in the default activity for quantifying enzyme of the embodiment and to set up the correlation of the stability with polluting residue removing performance and enzyme；If if concentrate, then using dilute solution.With known unit or known amylase assay and/or commercially available analysis method, such as Phadebas can be passed through

The activity of the amylase used in alpha-amylase analysis method, the expression present invention.

Naturally, the mixture of different amylase can be included in the present invention.Although the foregoing describing various specific enzymes, it is to be understood that can be used can assign composition needed for amylase activity any amylase, and do not limited anyway by specifically chosen amylase the present invention this embodiment.

Cellulase

Plant, animal or microorganism can be derived from by being suitable for the cellulase of the present composition.Preferably, cellulase is derived from microorganism, such as fungi or bacterium.Suitable cellulase includes those derived from fungi, such as Humicola insolens, Humicola strain DSMs 1800, or produces the fungi of cellulase 212 (it belongs to zygosaccharomyces)；With those extracted in the hepatopancrease of the auricle spire (Dolabella Auricula Solander) belonged to from sea mollusk truncation sea hare.Cellulase can be purified or can be the component of extract, and or be agriotype or variant (chemistry or the variant of restructuring).

The example for the cellulase that can be used in the compositions of the present invention is included with trade name Carezyme

Or Celluzyme

Those sold by Novo, or the Cellulase sold by Genencor；And analog.The mixture of cellulase can also be used.Suitable cellulase is disclosed in following patent documents：Novo United States Patent (USP) No.4435307, GB-A-2075028, GB-A-2095275, DE-OS-2247832, WO9117243 and WO9414951A (stable cellulase).

In one embodiment, the amount ranges for being present in the plain enzyme of the commercial fibres in the present composition are the about 0.1wt% to about 3wt% of decontaminating solution, preferably from about 1% to about 3wt% commercial enzyme product solution.Typical commercially available detergency enzymes include about 5-10% organized enzyme.

Although the percetage by weight for determining required cellulase be used for present invention teach that preparation embodiment be convenient in practice, but the change of commercial fibres element enzyme concentrate and environment additive on the spot and the negative effect to cellulase activity may require the more accurate analytical technology for cellulase assay, so as in the default activity for quantifying enzyme of the embodiment and foundation and the correlation for polluting residue removing performance and the stability of enzyme；If if concentrate, then using dilute solution.Can be with known unit or by known or commercially available cellulase assays, the activity of the cellulase used during expression is of the invention.

Naturally, the mixture of different cellulases can be included in the present invention.Although the foregoing describing various specific enzymes, it is to be understood that can be used can assign composition needed for cellulase activity any cellulase, and do not limited anyway by specifically chosen cellulase the present invention this embodiment.

Lipase

Plant, animal or microorganism can be derived from by being suitable for the lipase of the present composition.In one embodiment, lipase is derived from microorganism, such as fungi or bacterium.Suitable lipase includes being derived from pseudomonas, such as pseudomonas-stutzeri (Pseudomonasstutzeri) ATCC19.154, or those of Humicola, such as Humicola lanuginosa (it is typically by recombinant production in aspergillus oryzae (Aspergillus oryzae)).Lipase can be purified or can be the component of extract, and or be agriotype or variant (chemistry or the variant of restructuring).

The example for the lipase that can be used in the compositions of the present invention is included with trade name lipase P " Amano " or " Amano-P " by Amano Pharmaceutical Co., Ltd.s, those Japanese sold of Nagoya, or with trade name Lipolase

Those sold by Novo, and analog.The other commercially available lipase that can be used in the compositions of the present invention include Amano-CES, derived from Chromobacter viscosum (Chromobacter viscosum), (it is obtained from Toyo Jozo Co. to such as Chromobacter viscosum variant lipolyticum NRRLB3673 lipase, Tagata, Japan)；Obtained from U.S.Biochemical Corp., U.S.A. with Disoynth Co. Chromobacter viscosum lipase, and the lipase derived from gladiolus pseudomonas (Pseudomonas gladioli) or Humicola fine hair bacterium (Humicola lanuginosa).

Suitable lipase is with trade name Lipolase

Sold by Novo.Suitable lipase is disclosed in following patent documents：Novo WO9414951A (stable lipase), WO9205249, RD94359044, GB1372034, authorize Amano PharmaceuticalCo., Ltd. Japanese Patent Application Laid-Open 5320487, and EP341947 within 2 24th, 1978.

In one embodiment, the amount ranges for being present in the commercial lipase in the present composition are the about 0.1wt% to about 3wt% of decontaminating solution, preferably from about 1% to about 3wt% commercial enzyme product solution.Typical commercially available detergency enzymes include about 5-10% organized enzyme.

Although the percetage by weight for determining required lipase be used for present invention teach that preparation embodiment be convenient in practice, but the change of commercial lipase concentrate and environment additive on the spot and the negative effect to lipase activity may require the more accurate analytical technology for enzyme assay, in the default activity for quantifying enzyme of the embodiment and to set up the correlation of the stability with polluting residue removing performance and enzyme；If if concentrate, then using dilute solution.Can be with known unit or by known or commercially available lipase assays, the activity of the lipase used during expression is of the invention.

Naturally, the mixture of different lipase can be included in the present invention.Although the foregoing describing various specific enzymes, it is to be understood that can be used can assign composition needed for lipase activity any lipase, and do not limited anyway by specifically chosen lipase the present invention this embodiment.

Extra enzyme

The extra enzyme for being suitable for using in the present compositions includes cutinase, peroxidase, glucose enzyme and analog.Suitable cutinase is disclosed in Genencor WO8809367A.Known peroxidase includes horseradish peroxidase, lignoenzyme and haloperoxidase, such as chloro or bromoperoxidase.The peroxidase for being suitable for composition is disclosed in Novo WO89099813A and WO8909813A.Peroxidase can combine oxygen source, and such as percarbonate, perborate, hydrogen peroxide and analog are used.In GenencorInternational WO9307263A and WO9307260A, Novo WO8908694A, with McCarty et al. United States Patent (USP) No.3553139, the extra enzyme being suitable including through in the present composition is disclosed in Place et al. United States Patent (USP) No.4101457, Hughes United States Patent (USP) No.4507219 and Hora et al. United States Patent (USP) No.4261868.

It is suitable for the extra enzyme of the present composition, such as cutinase or peroxidase can be derived from plant, animal or microorganism.Preferably, the enzyme is derived from microorganism.Enzyme can be purified or can be the component of extract, and or be agriotype or variant (chemistry or the variant of restructuring).In a preferred embodiment of the invention, the amount ranges for being present in the commercial extra enzyme (such as cutinase or peroxidase) in the present composition are the about 0.1wt% to about 3wt% of decontaminating solution, preferably from about 1% to about 3wt% commercial enzyme product solution.Typical commercially available detergency enzymes include about 5-10% organized enzyme.

Although the percetage by weight for determining required extra enzyme (such as cutinase or peroxidase) be used for present invention teach that preparation embodiment be convenient in practice, but the change of the extra enzyme concentrate of business and environment additive on the spot and negative effect to its activity may require the more accurate analytical technology for enzyme assay, so as in the default activity for quantifying enzyme of the embodiment and foundation and the correlation for polluting residue removing performance and the stability of enzyme；If if concentrate, then using dilute solution.Can be with known unit or by known or commercially available analysis method, the extra enzyme used during expression is of the invention, the activity of such as cutinase or peroxidase.

Naturally, the mixture of different extra enzymes can be included in the present invention.Although the foregoing describing various specific enzymes, it is to be understood that can be used can assign composition needed for enzymatic activity any extra enzyme, and do not limited anyway by specifically chosen enzyme the present invention this embodiment.

The stabilising system of enzyme

The composition of the present invention may also comprise the composition of stable one or more enzymes.For example, the Cleasing compositions of the present invention may include the water-soluble source of calcium and/or magnesium ion.Calcium ion is generally more more effective than magnesium ion, and if if using an only cationoid, it is preferred herein.Composition, particularly liquid may include about 1 to about 30, preferably from about 2 to about 20, more preferably from about 8 to about 12mmol calcium ions/liter final composition, but various change is possible, and this depends on many factors, including the multiplicity, type and content of the enzyme added.Water-soluble calcium or magnesium salts are preferably used, including such as calcium chloride, calcium hydroxide, calcium formate, calcium malate, calcium maleate, calcium hydroxide and calcium acetate；More generally, calcium sulfate or the magnesium salts corresponding with calcium salt listed above can be used.Further the content of increase calcium and/or magnesium is certainly useful, such as the lubricating grease excision for promoting some type of surfactant is acted on.

Some Cleasing compositions, such as stabilising system in utensil washing composition can further comprise 0 to about 10%, preferably from about 0.01% to about 6wt% bleaching by chlorine gas scavengers, wherein add the bleaching by chlorine gas scavenger to prevent from being present in the water of many supplies bleaching by chlorine gas material attack enzyme and be allowed to inactivate, particularly in the basic conditions.Although the chlorine content in water may be smaller, typically scope is about 0.5ppm to about 1.75ppm, but for example during dish washing, obtainable chlorine may be relatively large in the entire volume of water contacted with enzyme, therefore enzyme is likely to become problem to the stability of the chlorine in.

Suitable chlorine cleaning agent anion is widely known and is readily available, and if if use, they can be the salt containing ammonium cation and inferior sulfate radical, bisulfite, hyposulfurous acid root, thiosulfate anion, iodide etc..Equally usable antioxidant such as carbaminate, ascorbate etc., organic amine such as ethylenediamine tetra-acetic acid (EDTA) or its alkali metal salt, MEA (MEA) and its mixture.Equally, it may include specific enzyme level system, there is maximum compatibility so as not to same enzyme.Visually need to use other conventional scavengers such as disulfate, nitrate, chloride, hydrogen peroxide source such as four hydrated sodium perborates, sodium perborate monohydrate and SODIUM PERCARBONATE and phosphate, condensed phosphate, acetate, benzoate, citrate, formates, lactate, malate, tartrate, salicylate etc. and its mixture.

In general, due to the function of chlorine cleaning agent can be exercised by the composition with known function independently listed, therefore do not require to add independent chlorine cleaning agent, unless the compound for exercising the function in required degree is not present in the embodiment containing enzyme of the present invention；Even if like that, addition scavenger is used for the purpose of optimal result.In addition, makers-up will avoid using any enzyme scavenger or stabilizer with the conventional skill of chemist, wherein the compatibility of the enzyme scavenger or stabilizer when preparing with other reactive ingredients is unacceptable.On the use of ammonium salt, this salt can be mixed simply with said composition, but during storage, it is easy to absorb water and/or discharges ammonia.Therefore, if present, it is protected in granular form needed to this material, as described in the United States Patent (USP) No.4652392 in Baginski et al..

Divalent ion

The Cleasing compositions of the present invention can contain divalent ion, such as calcium and magnesium ion, and its content is 0.05%-5wt%, the 0.1%-1wt% of composition weight, or about 0.25wt%.In one embodiment, calcium ion may include in the composition of the present invention.Calcium ion can be added for example with chloride, hydroxide, oxide, formates or acetate, or nitrate salts, be added preferably in the form of chloride, salt.

Polyalcohol

The microorganism formulation or Cleasing compositions of the stabilization of the present invention may also comprise polyalcohol.Polyalcohol can for example provide the extra stability of composition and hydrotropic performance.Suitable polyalcohol includes glycerine；Dihydric alcohol, such as ethylene glycol, propane diols or hexylene glycol；Sorbierite；Alkyl poly glucoside；And its mixture.In one embodiment, polyalcohol includes propane diols.

Include that there is those of the formula according to suitable alkyl poly glucoside of the present invention as polyalcohol：

(G)_x-O-R

Wherein G is the part derived from the reduced sugar containing 5 or 6 carbon atoms such as pentose or hexose, and R is the fatty aliphatic group containing 6-20 carbon atom, and x is the degree of polymerization (DP) of glycan glycosides, and it represents the quantity of the monosaccharide repeating units in glycan glycosides.Preferably, x is about 0.5 to about 10.In one embodiment, R contains 10-16 carbon atom, and x is 0.5-3.

In one embodiment, polyalcohol can be polyethers form.Suitable polyethers includes polyethylene glycol.Suitable polyethers includes those listed with solvent or cosolvent form.

In some embodiments, composition of the invention includes about 2 to about 30wt% polyalcohol, about 2 to about 10wt% polyalcohol, about 5 to about 20wt% polyalcohol, about 5 to about 10wt% polyalcohol, or about 10 to about 20wt% polyalcohol.In some embodiments, the microorganism formulation of the stabilization of the present invention includes about 2 to about 40wt% polyalcohol, about 2 to about 20wt% polyalcohol, about 2 to about 15wt% polyalcohol, about 2 to about 10wt% polyalcohol, about 3 to about 10wt% polyalcohol, about 4 to about 15wt% polyalcohol, or about 4 to about 8wt% polyalcohol, about 4wt% polyalcohol, about 8wt% polyalcohol, or about 12wt% polyalcohol.Said composition may include not with any one in these scopes or consumption about changed.

Solvent or cosolvent

Solvent or cosolvent can be used with the removal capacity for certain dirt for improving the present invention.It is preferred that cosolvent be alcohol and aklylene glycol, two aklylene glycols, the list and dialkyl ether of three aklylene glycols etc..Methanol, ethanol, propyl alcohol and isopropanol can be included by the alcohol as cosolvent in the present invention.Be used especially for the present invention is the list and dialkyl ether of ethylene glycol and diethylene glycol (DEG), and they obtain the popular name of such as poly- glyme, cellosolve and carbitol etc.The representative example of this group of cosolvent includes methyl cellosolve, butyl carbitol, dibutyl carbitol (DBC), diethylene glycol dimethyl ether, triglyme etc..Nonaqueous liquid solvents can be used to change the composition of the present invention.These include higher glycols, poly-dihydric alcohol, poly oxide and glycol ether.Suitable material is propane diols, polyethylene glycol, polypropylene glycol, diethylene glycol monoethyl ether, diethylene glycol monopropyl ether, diethylene glycol monobutyl ether, tripropylene glycol methyl ether, propylene glycol monomethyl ether (PM), dipropylene glycol methyl ether (DPM), propylene glycol methyl ether acetate (PMA), dipropylene glycol methyl ether acetic acid esters (CPMA), ethylene glycol n-butyl ether and ethylene glycol n-propyl ether.Other useful solvents are ethylene oxide/propylene oxide, Synalox of the liquid randam copolymer as being obtained from Dow Chemical

Solvent series are (for example, Synalox

50-50B).Other suitable solvents are propylene glycols, and such as PnB, DPnB and TPnB are (by Dow Chemical with trade name Dowanol

Propylene glycol monobutyl ether, Dipropylene glycol mono-n-butyl Ether and the Tri(propylene glycol)butyl ether,mixture of isomers of sale).Tripropylene glycol monomethyl Ether " Dowanol TPM obtained from Dow Chemical

" it is also suitable.

Suitable solvent to be used in conjunction with the invention includes non-VOCs or low VOCs, including DPnB, PnB, orange limonene, 1-METHYLPYRROLIDONE, propane diols phenyl ether, ethylene glycol phenyl ether, tripropylene glycol methyl ether etc..

Acidulant

Using acidulant or base reagent with maintain the present invention the suitable pH of cleaning agent.Carefully control pH can improve spatter property.Preparing acidic components used in the cleaning agent of the present invention or acidulant includes may be dissolved in the acid in the Aquo System of the present invention, so as to downward regulation pH.Preferably, common commercially available weak inorganic acid and organic acid can be used in the present invention.Useful weak inorganic acid includes phosphoric acid and sulfamic acid.Useful weak organic acid includes acetic acid, hydroxyacetic acid, citric acid, tartaric acid and analog.It was found that useful acidulant includes organic and inorganic acid, such as citric acid, lactic acid, acetic acid, hydroxyacetic acid, adipic acid, tartaric acid, butanedioic acid, propionic acid, maleic acid, alkanesulfonic acid, cycloalkane sulfonic acids, and phosphoric acid and analog or its mixture.

Extra alkali source

Basic matterial available for regulation pH includes both weak and strong basic matterials.This material generally comprises highly basic such as NaOH, potassium hydroxide, alkali metal salt such as sodium carbonate, potassium carbonate, sodium acid carbonate, saleratus, concentrated crystal soda, Boratex, potassium borate, sodium phosphate and potassium phosphate, organic bases such as triethanolamine, tripropanol amine etc., alkali silicate, alkali metal salt.

Extra alkali source may include potassium hydroxide or alkaline potassium salt such as potassium carbonate, saleratus, potassium phosphate etc..

Thickener or gelling agent

Suitable thickener may include not contain those of the component incompatible with the food in contact area or other sensitive products.In addition, thickener should not suppress the growth of spore of the present invention.In general, the thickener that can be used in the present invention includes natural gums such as xanthans, guar gum, modified guar or other glue from plant mucilage；Modified adhesive；Polysaccharide-based thickener, such as alginates, starch and cellulosic polymer (for example, carboxymethyl cellulose, hydroxyethyl cellulose and analog)；Polyacrylic ester thickener；Associative thickener；And hydrocolloid thickeners, such as pectin.In general, the concentration of the thickener used in the present composition or method is determined by viscosity required in final composition.However, as general guidance, the concentration range of the thickener in the present composition is about 0.05wt% to about 3wt%, and about 0.1wt% is to about 2wt%, or about 0.1wt% to about 0.5wt%.

Dyestuff

The composition of the present invention may also comprise dyestuff.Dyestuff advantageously provides the visibility of the product in packaging, distributor and/or circuit to composition.Various dyestuffs are suitable, including ACID GREEN 25 and direct indigo plant 86.

Use composition

The compositions and methods of the invention are suitable for removing the organic or greasy soils and mineral contaminants of complexity from various substrates.Degreasing aqueous solution can be formed undoped using the composition (i.e. no diluent, such as aqueous diluent) of the present invention, or available water or the composition of other liquid mediums dilution present invention.In addition, the grease removing composition of the invention as additive can be used together with the Cleasing compositions of other preparations for clean substrate.

The additive that the organic and mineral contaminants Cleasing compositions of the removing lubricating grease of the present invention can be used as removing lubricating grease is used in the cleaning material of preparation.This cleaning material is industrial common, and including hard surface cleaners, cloth-washing detergent, for all purpose cleaner used in family and mechanism application, ground cleaner, glass cleaner etc..By the composition of the present invention of addition about 0.1 to about 20wt% into conventional cleaning agent prescription, the composition of the present invention is used as additive.The material of the present invention, even when individually consumingly diluting in aqueous or in the formula of such as glass cleaner, hard surface cleaners, all purpose cleaner or cloth-washing detergent etc, particularly preferred lubricating grease removing performance can be provided, it is almost effective as concentrate material.

Can full strength (pure, i.e., in the absence of aqueous diluent in the case of) using the present invention composition.The composition of the present invention can be applied directly to typically on hard surface, the organic or greasy soils such as glass, metal, composite, timber on surface.The present composition combined with organic or greasy soils tends to reduce the interfacial adhesion of any dirt/hard surface and reduces the cohesion of complicated dirt and reduce the viscosity of waste material, so as to cause relatively easy physics to remove.

It may include any wt% contents divided by amount of dilution of composition listed above using composition, and can be expressed with wt% or ppm.Especially, for borate and microbial components or spore, content listed above is the content of concentrate composition.For example, may include any wt% contents listed above independently divided by 10,20,30,40,50,60,70,80,90,100,200,300,400,500,600,700,800,900,1000,2000,3000,4000,5000,6000,7000,8000,9000 or 10000 using composition.In one embodiment, dilution method is that 2oz concentrate increases to 1 gallon and uses composition.

Foam

In one embodiment, composition of the invention can be mixed with diluent, and what formation was used in foam maker uses composition.The foam maker that for example can be installed using foam applying device, such as pot type foam maker or air-breathing wall, such as, using the foam maker nozzle of triggering sprayer, realize the application of foam.It can be placed by applying in 15 gallons of foam in pressure vessel, such as in the Stainless steel pressure vessels of 15 Gallon Capacities with mixing auger oar and use composition, so as to realize the application of foam.Then the foam compositions can be distributed by foaming trigger sprayer.Air can be used to discharge foam from tank or pipeline for the foam maker that wall is installed.In one embodiment, the air of compression can be expelled in mixture, then by foam applying device, the foam maker that such as pot type foam maker or air-breathing wall are installed is applied on object.

Can the mechanical foaming head used according to the invention to provide foam generation include causing air and foam compositions mix and the composition that produces foam those heads.That is, mechanical foaming head causes air and foam compositions to mix in mixing chamber, then pass through opening to generate foams.

Suitable mechanical foaming heads that can be used according to the invention include being obtained from PompanoBeach, Florida Airspray International, Inc, and obtained from ZellerPlastik (Crown Cork and Seal Co. branch company) those.Disclosed in such as United States Patent (USP) No.D-452822, United States Patent (USP) No.D-452653, United States Patent (USP) No.D-456260 and United States Patent (USP) No.6053364 can be used according to the invention suitable mechanical foaming head.Mechanical foaming head that can be used according to the invention is included by applying those heads that finger pressure drives or driven to trigger, to cause foam compositions and air to mix and produce foams.That is, the finger pressure of people can depression of trigger, enter in head so as to draw foam compositions and air and cause foam compositions and air to mix and produce foams.

Use the method for the present composition

In one embodiment, Cleasing compositions are applied directly on serious soil deposit so that softening dirt simultaneously promotes dirt to remove.Once so that composition improves the removeability of dirt, then cleaning agent and the dirt removed can be removed easily using rinse step.In one embodiment, this method includes saving rinsing.That is, the composition and no rinsed surfaces of the present invention can be applied.It directly can contact to remove organic, oily or greasy soils with hard surface containing nonionic surfactant, nonionic siloxane surfactant, anion surfactant and the hydrotropic present composition.Depending on substrate, this composition can comprise additionally in chelating agent, so that end formulation includes nonionic surfactant and nonionic siloxane surfactant, anion surfactant, hydrotrope solubilizers and chelating agent.Can be on substantially non-corrosive surface, such as plastics, timber, the timber of coating, stainless steel, composite, fabric, cement and other upper these compositions of use.

In one embodiment, the method for the present invention includes the method on cleaning hard surface.This method may include to apply containing spore or bacterium, borate, about 0.5 to about 35wt% nonionic surfactant and about 0.1 to the Cleasing compositions of about 35wt% silicone surfactant to surface.This method may include apply said composition to ground, drainpipe or its combine it is upper.

In one embodiment, the method for the present invention includes the method on cleaning ground.This method may include to increase the coefficient of friction on ground.This method may include the mortar for cleaning tile floor.Cleaning mortar may include to make it show nature color.This method includes applying the Sporess thing of the stabilization of the present invention on ground.In one embodiment, this method does not include (for example saving) rinsing.In one embodiment, the method for the present invention may include effectively to remove the film slided when wet from earth material (such as ceramic tile).This method may include cleaning earth material and increase its coefficient of friction.

In one embodiment, the method on cleaning hard surface of the present invention may include the composition for applying the present invention to bathroom surfaces, such as on wall, ground or furniture.Bathroom surfaces can be shower wall or surface.Bathroom surfaces can be the wall of tiling.The composition used on a vertical surface may include thickener, wetting agent or the surfactant of foaming.Apply composition may include to make composition foam to vertical surface.In one embodiment, composition of the invention include thickener or wetting agent, they can aid composition be retained on horizontally or vertically surface.

In one embodiment, the method for the present invention may include that the composition for applying the present invention has on the surface of lubricating grease or oil to thereon.This surface includes ground, parking lot, be open to traffic pad, garage floor, parking ramp floor and analog.

In one embodiment, the method for the present invention includes the composition sprayed with the present invention or the surface of spraying.

In one embodiment, the method for the present invention includes applying stable microbial composite to surface and is kept for period of the surface moisture length, and such as 1 or 2 hour until about 8-16 hours.It can realize that holding surface is moist by applying composition repeatedly, such as by spraying.The period of length can be contacted with sponge, rag or the mop soaked with said composition by making the surface, realize that holding surface is moist.It can realize that holding surface is moist by applying the microbial composite of lasting stability.The microbial composite of lasting stability is positively retained on the surface and the surface is kept moist.For example, the composition of thickening and the composition of some foaming are positively retained on the surface and the surface is kept moist.Compared with the composition dried or evaporated, the composition of the long-term existence present invention, which can be provided, faster to be cleaned.

With reference to following embodiments, the present invention is better understood with.These embodiment work done in the manner of a certain author are representational specific embodiment of the invention, and are not intended to be limiting the scope of the present invention.

Embodiment

Embodiment 1- borate stabilised microorganism compositions

The composition stabilised microorganism preparation of the present invention is proved, the Sporess thing of lubricating grease is specifically digested.

Materials and methods

The experimental evaluation is in the case of ageing and not aged composition, by the aerobic plate count of the various Cleasing compositions production containing bacterial spore.Those compositions containing spore living produce the bacterial clump with lipolytic activity, and the bacterial clump causes the dark areas in plated growth media.Dark areas comes from the generation of free fatty.Tester includes being suspended in water and the spore in conventional bacterial cleaning compositions or bacterium.

The test method be according to " Lipolytic Microorganisms ",Compendium of Methods for the Microbiological Examination of Foods, the 3rd edition, the standard scheme of page 1992,183.In brief, steatolysis agar plate is prepared.The flat board is inoculated with experiment bacterial suspension and is allowed to drying.Nutrient agar is poured on inoculated surface.The flat board is cultivated at room temperature, makes bacterial growth, and detect the outward appearance of lipolytic colonies.By identifying lipolytic colonies around skipper region.

In this embodiment, prepare and test following compositions：

 

	1	2	3	4	5	6	7
								Water	26	28	28	30	54	56	56
Boric acid	10	10	10	10
								Alkanolamine	19	19	19	19	2	2	2
Polyalcohol	8	8	8	8	8	8	8
								Nonionic surfactant	8	8	8	8	8	8	8
Silicone surfactant	3	3	3	3	3	3	3
								Amphoteric surfactant	5	5	5	5	5	5	5
Anion surfactant	8	8	8	8	8	8	8
								Hydrotropic agent	11	11	11	11	11	11	11
Spore blend	2	2			2		2
								Protease	2		2		2	2

All consumptions are in terms of wt%

Reference composition 8 is included in the 2wt% spore blends in water.Reference composition 9 is included in the 2wt% protease in water.Reference composition 10 is included in the protease of the 2wt% spore blends and 2wt% in water.

Each composition is diluted to growths of the 2wt% to test bacterium.

As a result

Table 1-3 is reported in compositions as described above, in control formula and in commercially available formula, the test result of spore blend viablity.

Table 1

 

(formula number) explanation	Aerobic steatolysis plate count (CFU/mL)
		The spore blend of water+2%	5.1×104
The protease of water+2%	<1
		The protease of the spore blend of water+2%+2%	9.8×103
(1) protease of+2% spore blend of amine borate+2%	4.7×104
		(2) spore blend of amine borate+2%	7.6×104
(3) protease of amine borate+2%	1.7×102*
		(4) amine borate	<1
(5) without the protease of the spore blend of borate+2%+2%	2.3×104
		(6) without the protease of borate+2%	1.3×102*
(6) without the spore blend of borate+2%	2.5×104

* cross pollution between=sample

Table 2

 

(formula number) explanation	Aerobic plate count result (CFU/mL)
		(1) the freshly prepd protease of+2% spore blend of amine borate+2%	1.7×104
(1) it is aged the protease of+2% spore blend of amine borate+2% of 6 days	2.1×104
		(5) it is freshly prepd without the protease of the spore blend of borate+2%+2%	2.5×104
(5) be aged 6 days without the protease of the spore blend of borate+2%+2%	2.0×103
		2% commercial spore blend containing cleaning agent of time is not known	5.0×102
2% commercial spore blend containing cleaning agent at 4 monthly ages	3.6×103
		The spore blend of water+2%	3.0×104

Table 3

 

(formula number) explanation	Aerobic plate count result (CFU/mL)
		(1) it is aged the protease of+2% spore blend of amine borate+2% of 10 weeks	2.1×104
(1) it is aged the protease of+2% spore blend of amine borate+2% of 5 weeks	1.4×104
		(1) it is aged the protease of+2% spore blend of amine borate+2% of 4 weeks	2.0×104
(5) be aged 4 weeks without the protease of the spore blend of borate+2%+2%	1.3×104
		(5) being aged 5 weeks does not have the protease of+2% spore blend of amine borate+2%	2.4×103
The ground cleaner containing 2 parts of spores of business, freshly prepd concentrate	3.8×105

Conclusion

The spore of the bacterium of the stable digestion lubricating grease of amine borate and bacterium are in itself.For the concentrate composition of borate containing amine and spore blend, it was observed that increased stability.For example, the bacterial activity of about 1 logarithm of sample loss in 6 day age of 5 (not containing borates) of formula.Unexpected, the sample for being formulated 6 day age of 1 (it includes amine borate) keeps complete bacterial activity.That is, it keeps the activity as freshly prepd sample.

It is significant in the deterioration of cleaning agent (its not containing borate) interior bacterial activity containing commercial spore blend.The bacterial activity of about 1 logarithm of the sample loss at 4 monthly ages.The bacterial activity of about 2 logarithms of sample loss of time is not known.

Unexpected, amine borate (it may have limited solubility) is solvable in the composition with silicone surfactant.

Borate compositions stabilised microorganism preparations of the embodiment 2- containing polyalcohol

Prove, according to the present invention and including borate and polyalcohol combination thing stabilised microorganism preparation, specifically to digest the Sporess thing of lubricating grease.

Materials and methods

Using the borate counter ion (such as alkanolamine) and polyalcohol (such as propane diols) of various concentration, composition is prepared according to the general formulation listed in embodiment 1.By measuring the lipolytic activity in different time after composition is prepared, the stability of composition is determined.Said composition usually contains 2wt% spore blend.Dilute each composition to growths of the 2wt% to test bacterium, this as described in example 1 above as.

In this embodiment, prepare and test following compositions：

 

	11	12	13	14	15	16	17	18	19	20
											Water	50	44	38	31	48	36	58	55	49	43
Boric acid	2	4	6	8	4	8	2	4	6	8
											Alkanolamine	5	9	14	18	9	18	5	9	14	18
Polyalcohol	8	8	8	8	4	4
											Nonionic surfactant	8	8	8	8	8	8	8	8	8	8
Silicone surfactant	3	3	3	3	3	3	3	3	3	3
											Amphiphilic surfactant	5	5	5	5	5	5	5	5	5	5
Anion surfactant	8	8	8	8	8	8	8	8	8	8
											Hydrotropic agent	11	11	11	11	11	11	11	8	8	8
Spore blend	2	2	2	2	2	2	2	2	2	2
											Protease				1
											PH100%	9.7	10	10.3	10.1	10.1	10.5	10.1	10	10.4	10.5
PH1%	9.2	9.3	9.5	9.3	9.3	9.5	9.5	9.4	9.4	9.5

All consumptions are in terms of wt%

As a result

Table 4-8 reports the test result in composition 11-20 miospore blend viablities.

Composition non-aged table 4-

 

(formula number) explanation	Aerobic plate count result (CFU/mL)	Growth declines (logarithm)
			(11) 2% boric acid+5%MEA+8% propane diols	4.5×104	NA
(12) 4% boric acid+9%MEA+8% propane diols	3.0×103	NA
			(13) 6% boric acid+14%MEA+8% propane diols	2.2×104	NA
(14) 8% boric acid+18%MEA+8% propane diols	2.0×104	NA
			(15) 4% boric acid+9%MEA+4% propane diols	2.4×104	NA
(16) 8% boric acid+18%MEA+4% propane diols	2.8×104	NA
			(17) 2% boric acid+5%MEA	5.4×104	NA
(18) 4% boric acid+9%MEA	5.0×104	NA
			(19) 6% boric acid+14%MEA	2.7×104	NA
(20) 8% boric acid+18%MEA	3.4×104	NA

Table 5- is aged the composition of 4 weeks

 

(formula number) explanation	Aerobic plate count result (CFU/mL)	Growth declines (logarithm)
			(11) 2% boric acid+5%MEA+8% propane diols	2.2×105	-
(12) 4% boric acid+9%MEA+8% propane diols	9.4×104	-
			(13) 6% boric acid+14%MEA+8% propane diols	1.2×105	-
(14) 8% boric acid+18%MEA+8% propane diols	1.2×105	-
			(15) 4% boric acid+9%MEA+4% propane diols	3.2×105	-
(16) 8% boric acid+18%MEA+4% propane diols	1.0×105	-
			(17) 2% boric acid+5%MEA	1.9×105	-
(18) 4% boric acid+9%MEA	2.5×104	0.3
			(19) 6% boric acid+14%MEA	4.8×104	-
(20) 8% boric acid+18%MEA	1.0×105	-

Table 6- is aged the composition of 8 weeks

 

(formula number) explanation	Aerobic plate count result (CFU/mL)	Growth declines (logarithm)
			(11) 2% boric acid+5%MEA+8% propane diols	2.1×104	0.33
(12) 4% boric acid+9%MEA+8% propane diols	3.0×104	-
			(13) 6% boric acid+14%MEA+8% propane diols	2.2×103	1.0
(14) 8% boric acid+18%MEA+8% propane diols	3.4×104	-
			(15) 4% boric acid+9%MEA+4% propane diols	3.3×104	-
(16) 8% boric acid+18%MEA+4% propane diols	1.3×104	0.33
			(17) 2% boric acid+5%MEA	1.8×104	0.48
(18) 4% boric acid+9%MEA	2.7×104	0.27
			(19) 6% boric acid+14%MEA	5.0×103	0.72
(20) 8% boric acid+18%MEA	6.0×103	0.75

Table 7- is aged the composition of 12 weeks

 

(formula number) explanation	Aerobic plate count result (CFU/mL)	Growth declines (logarithm)
			(11) 2% boric acid+5%MEA+8% propane diols	1.1×104	0.61
(12) 4% boric acid+9%MEA+8% propane diols	5.2×103	-
			(13) 6% boric acid+14%MEA+8% propane diols	5.4×102	1.61
(14) 8% boric acid+18%MEA+8% propane diols	1.4×102	2.15
			(15) 4% boric acid+9%MEA+4% propane diols	6.8×103	0.55
(16) 8% boric acid+18%MEA+4% propane diols	1.5×101	3.27
			(17) 2% boric acid+5%MEA	2.4×103	1.35
(18) 4% boric acid+9%MEA	3.2×103	1.19
			(19) 6% boric acid+14%MEA	5.1×102	1.72
(20) 8% boric acid+18%MEA	<1×101	3.53

Table 8- is aged the composition of 16 weeks

 

(formula number) explanation	Aerobic plate count result (CFU/mL)	Growth declines (logarithm)
			(11) 2% boric acid+5%MEA+8% propane diols	6.2×103	0.86
(12) 4% boric acid+9%MEA+8% propane diols	2.0×103	0.18
			(15) 4% boric acid+9%MEA+4% propane diols	5.8×102	1.62

Conclusion：

After aged composition up to 8 weeks, occur only slightly decreasing for bacterial growth.Observe that the more significant of bacterial growth declines after being aged 12 weeks.For example, for composition no 13,14,16,17,18,19 and 20, the decline of bacterial growth is more than or equal to 1 logarithm.This means composition no 11,12 and 15 shows the stability of maximum after being aged 12 weeks.These results prove borate (for example, alkanolamine borate) resistant spore blend.

Interestingly, each composition without polyalcohol shows the decline more than 1 logarithm.This shows that polyalcohol contributes to resistant spore blend.

Interestingly, composition of the invention is even more than under 9.5, such as pH10 in pH, and the resistant spore blend even under pH10.5.For example, resistant spore blend up to 16 weeks under about pH10 of composition 12.

Embodiment 3- borate compositions stabilised microorganism preparations at basic ph

Prove according to the present invention and including borate and polyalcohol combination thing under the alkaline pH of wide scope stabilised microorganism preparation, specifically digestion lubricating grease Sporess thing.

Materials and methods

Using the pH of change, composition is prepared according to the general formulation listed in embodiment 1.By measuring the lipolytic activity in different time after composition is prepared, the stability of composition is determined.Said composition usually contains 2wt% spore blend.Dilute each composition to growths of the 2wt% to test bacterium, this as described in example 1 above as.

In this embodiment, prepare and test following compositions：

 

	21(pH7)	22(pH7.5)	23(pH8)	24(pH8.5)	25(pH9)
						Water	55	55	54	53	53
Boric acid	4	4	4	4	4
						Alkanolamine	2	2.5	3	4	4.5
Polyalcohol	4	4	4	4	4
						Nonionic surfactant	8	8	8	8	8
Silicone surfactant	3	3	3	3	3
						Amphoteric surfactant	5	5	5	5	5
Anion surfactant	8	8	8	8	8
						Hydrotropic agent	11	11	11	11	11
Spore blend	2	2	2	2	2

All consumptions are in terms of wt%

As a result

Table 9 and 10 reports the test result in composition 21-25 miospore blend viablities.

Table 9- steatolysis microorganism count (CFU/mL)-copy board is averaged

 

Composition	It is non-aged	Ageing 4 weeks	Ageing 8 weeks	Ageing 14 weeks	14 weeks (24 hours) *
						21pH7	1.9×103	4.4×103	1.4×103	1.3×103	1.8×103
22pH7.5	3.2×103	7.8×103	5.4×102	1.3×103	1.2×103
						23pH8	1.2×103	7.4×103	2.6×103	2.0×103	2.1×103
24pH8.5	2.0×104	7.5×103	1.2×103	1.4×103	9.0×102
						25pH9	2.1×103	1.4×104	2.4×103	1.7×103	2.0×103

* concentrate is aged 14 weeks, and the use composition of dilution is aged 24 hours

Table 10- declines (in units of logarithm) in the growth after ageing

 

Composition	It is non-aged	Ageing 4 weeks	Ageing 8 weeks	Ageing 14 weeks	14 weeks (24 hours) *
						21pH7	NA	-	0.13	0.16	0.02
22pH7.5	NA	-	0.77	0.39	0.42
						23pH8	NA	-	-	-	-
24pH8.5	NA	0.43	1.22	1.15	1.35
						25pH9	NA	-	-	0.09	0.02

* concentrate is aged 14 weeks, and the use composition of dilution is aged 24 hours

Conclusion

For the ageing of at least about 14 weeks, the present composition of containing borate and polyalcohol provides effective stability under pH7-9 to spore blend.

The microbial composite of stabilizations of the embodiment 4- added with lipase

Prepare according to the present invention and include the composition of borate, polyalcohol and lipase, and be shown to be stable and effective cleaning agent (composition 26 and 27).Composition containing these lipase, which includes the composition of following dosage and the composition (28-31) containing extra lipase, may include the composition of following dosage：

 

	26	27	28	29	30	31	32	33
									Water	27	64	60	56	52	48	64	52
Boric acid	10	5	5	5	5	5	5	5
									Alkanolamine	18	9	9	9	9	9	9	8
Polyalcohol	8	4	8	12	16	20	4	12
									Nonionic surfactant	8	4	4	4	4	4	4	4
Silicone surfactant	3	1	1	1	1	1	1.3	1.3
									Amphoteric surfactant	5	3	3	3	3	3	3	3
Anion surfactant	8	4	4	4	4	4	4	4
									Hydrotropic agent	11	3	3	3	3	3	3	3
Sequestering agent								4
									Spore blend	2	1	1	1	1	1	1	1
Lipase	2	1	1	1	1	1	1	2

All consumptions are in terms of wt%

Microbial composite stable embodiment 5- increases the skid resistance on ground

Show that according to the present invention and composition including borate, polyalcohol and lipase be effective for dramatically increasing the skid resistance of tile floor.

Materials and methods

Daily to tile floor, specifically apply the use dilution (2oz/gal or 1.6% concentrate) containing composition 33 on clinker tile ground and do not rinse.Skid resistance when drying and moistening is measured within the period of 6 weeks in the kitchen in 5 restaurants.Include within this 6 weeks base line measurement in 2 weeks and measuring for 4 weeks after composition 33 is applied.Before with the cleaning of the composition of the present invention (such as before the neutralization of baseline time section), daily with conventional commercially available floor cleaning composition cleaning ground.

According to ASTM F1679-02, using Britain XL variable incidence friction gauges, skid resistance is measured in coefficient of friction (COF) form.Scheme is as described below.15 pieces of clinker tiles are selected in the kitchen in each restaurant.Every 5 pieces of ceramic tile selections in main track route of concern and region (for example, close to frying pan).The COF of 15 blocks of ceramic tiles of identical in each restaurant is evaluated weekly.The COF of each ceramic tile is measured 4 times, is being separated by measurement on one of 90 degree 4 directions each time.Each ceramic tile is measured in the case of drying and moistening.It is average to measure 60 times under each condition for each restaurant, and take the average value of the result in 5 restaurants.

As a result

Fig. 1 show weekly in one of 5 restaurants for 15 blocks of ceramic tiles COF (skid resistance) acquired results.By the testing time section of 4 weeks, the COF for drying ceramic tile was improved to 0.81 from Average baseline values 0.60.By the testing time section of 4 weeks, the COF of moistening ceramic tile was improved to 0.56 from Average baseline values 0.38.These value addeds are significant and confidence level is more than 99%.

Conclusion

For easily sliding surface, such as the ground in restaurant kitchen, coefficient of friction is dramatically increased according to the composition of the present invention.

Microbial Compositions Clean mortar stable embodiment 6-

Show that according to the present invention and composition including borate, polyalcohol and lipase be effective for the mortar between cleaning ceramic tile.

Measurement and method

As described in embodiment 5, to tile floor, specifically apply the use dilution (2oz/gal or 1.6% concentrate) containing composition 33 on clinker tile ground and do not rinse.Before and after the composition of the present invention is applied, taken pictures to ceramic tile.

As a result

Fig. 2A and 2B photo explanation, composition of the invention cleans the mortar on restaurant kitchen inner casing brick flooring.Fig. 2A illustrates the ground before the composition of the present invention is applied.Fig. 2 B illustrate the ground after the composition of the present invention is applied.

Fig. 3 illustrates a part of ground cleaned with conventional Cleasing compositions (left side) and the part cleaned with composition 33.Composition 33 cleans mortar, and conventional composition does not have.

Conclusion

The composition of the present invention more efficiently cleans tile grout than conventional composition.

Stable composition thing cleaning ground stable embodiment 7-

Show that according to the present invention and composition including borate, polyalcohol and spore be effective for cleaning ground.

Materials and methods

To tile floor, specifically apply the use dilution (2oz/gal or 1.6% concentrate) containing composition 34 on clinker tile ground and do not rinse.Before and after the composition of the present invention is applied, ground is evaluated.

 

	34
		Water	43
Boric acid	10
		Alkanolamine	19
Polyalcohol	5
		Nonionic surfactant	5
Silicone surfactant	2
		Amphoteric surfactant	3
Anion surfactant	5
		Hydrotropic agent	7
Spore blend	1

All consumptions are in terms of wt%

As a result

Composition 34 cleans ground.

Conclusion

The composition of the present invention more efficiently cleans ground than conventional composition.

It should be noted that the odd number " one ", "the" and " this (a, an, the) " used in this specification and the appended claims include plural form, unless the content clearly refers else.Thus, for example the mixture of two or more compounds should be included containing the composition with reference to " a kind of compound ".It shall also be noted that term "or" is generally used with the implication including "and/or", unless the content clearly refers else.

All public publications and patent application in this manual are the expressions for belonging to those skilled in the art in the invention's level.

With reference to various specific and preferred embodiments the present invention is described with technology.It will be appreciated, however, that can many modifications may be made and modified, and keep within the spirit and scope of the present invention simultaneously.

Claims (20)

1. a kind of method on cleaning ground, including：

A composition) is applied to ground, the composition is included：

I) spore or bacterium；

Ii) enzyme；

Iii) alkanolamine borate；

Iv) anion surfactant；With

V) nonionic surfactant, wherein the alkanolamine borate exists with 5 to 35wt% concentration；With

B composition a period of time on the ground) is retained.

2. the method for claim 1, also including removing the composition from ground.

3. the method for claim 1 wherein do not rinse the composition from ground.

4. the method for claim 1 wherein ground selected from following materials by being made：Plastics, timber, timber, stainless steel, composite, fabric, cement and the ceramic tile of coating.

5. the method for claim 1 wherein ground includes mortar.

6. the method for claim 1 wherein the pH of composition is more than or equal to 8.

7. the method for claim 1 wherein composition further includes polyalcohol.

8. the method for claim 7, wherein polyalcohol include propane diols.

9. the method for claim 1 wherein composition is further comprising most about 65wt% water.

10. the method for claim 1 wherein alkanolamine borate includes monoethanol ammonium borate, diethanol ammonium borate, three ethanol ammonium borates or its combination.

11. the method for claim 1 wherein composition includes about 10-30wt% alkanolamine borate.

12. the method for claim 1 wherein spore includes bacterial spore.

13. the method for claim 1 wherein composition includes about 0.003-35wt% nonionic surfactant.

14. claim 1 method, wherein nonionic surfactant are selected from：

Containing at least (EO)_y(PO)_zNon-ionic block copolymer, wherein y and z independently are 2 to 100；

C with 2-15mol oxirane_6-24Alkyl phenol alkoxylate；

C with 2-15mol oxirane_6-24Alcohol alkoxide；

Alkoxylated amines with 2-20mol oxirane；

Or its mixture.

15. the method for claim 1 wherein composition further includes about 0.0005-35wt% silicone surfactant.

16. the method for claim 15, wherein silicone surfactant include siloxane main chain and the epoxy alkyl with about 2-100mol alkylene oxides of at least one pendant.

17. the method for claim 1 wherein anion surfactant is selected from linear alkylbenzene sulfonate (LAS), alpha-alkene sulfonate, alkyl sulfate, secondary paraffin sulfonate, sulfosuccinate or its mixture.

18. the method for claim 1 wherein composition further includes about 0.001-20wt% hydrotropic agent.

19. the method for claim 18, wherein hydrotropic agent are selected from C_6-24Alkyl dimethyl amine oxide, alkylated dipheny ethers disulfonate or its mixture.

20. the method for claim 1 wherein enzyme is selected from protease, amylase, lipase, cellulase, peroxidase, glucose enzyme or its mixture.

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