CN101376906A - Production method for mixed nucleotides - Google Patents
Production method for mixed nucleotides Download PDFInfo
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- CN101376906A CN101376906A CNA2008101554841A CN200810155484A CN101376906A CN 101376906 A CN101376906 A CN 101376906A CN A2008101554841 A CNA2008101554841 A CN A2008101554841A CN 200810155484 A CN200810155484 A CN 200810155484A CN 101376906 A CN101376906 A CN 101376906A
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Abstract
The invention relates to a production method of mixed nucleotide, which subsequently comprises the following steps: RNA solution preparation; RNA solution zymohydrolysis; filtration; decoloring by a decoloring column; sodium filtration-concentration. The process conditions of the zymohydrolysis reaction of the RNA solution is strictly controlled, so that the total area of four main peaks 5'-adenylic acid, 5'-cytidylic acid, 5'-uridylic acid and 5'-guanylic acid in the high-pressure liquid chromatograph of the obtained ribonucleotide mixture reaches about 95 percent of the total peak area; solid particles are removed by filtration; impurities with smaller grain size are removed by micro-filtration; the product is decolored by the decoloring column, and the residual protein and other impurities in the solution are removed at the same time; partial water and impurities with small molecular weight are removed by sodium filtration-concentration. The purity of the solution after once and again purification is rather high, and the concentrated solution can be used as a mixed ribonucleotide product or receives further spray drying treatment to obtain mixed ribonucleotide powder. The method has the advantages of short process route, high yield, low cost, less waste discharge, high product content and good safety.
Description
Technical field
The present invention relates to a kind of production method of mixed nucleotides, particularly, Nucleotide prepares by enzyme process.
Background technology
In the market, Nucleotide is as nutritious prod, and the subject of a sale is mainly baby and old man.Nucleotide for baby has strict quality standard, is to be added in the infant formula after being mixed by a certain percentage with the mononucleotide through the chromatography post crystallization again.And just do not have standard as the Nucleotide quality of health care for the aged product.Once it is dark in strong tea to see liquid state " Nucleotide " color that is contained in the ampere bottle; Also once " Nucleotide " capsule of certain brand was carried out mass analysis, wherein the nucleotide content less than 10%.
Summary of the invention
Technical problem to be solved by this invention provides a kind of production method of mixed nucleotides, and is with short production cycle, energy consumption is little, environmental pollution is little, security is good, product yield is high, production cost is low.
For solving above technical problem, the present invention takes following technical scheme:
A kind of production method of mixed nucleotides may further comprise the steps successively:
(1), preparation rna solution: Yeast Nucleic Acid is added in the entry, add alkali simultaneously, make the Yeast Nucleic Acid dissolving;
(2), enzymolysis Yeast Nucleic Acid: described rna solution is transferred to neutrality, add 5 '-phosphodiesterase at a certain temperature, Yeast Nucleic Acid is broken down into 5 '-adenylic acid (AMP), 5 '-cytidylic acid, 5 '-uridylic acid and 5-guanylic acid, obtains the mixing solutions of Nucleotide;
(3), solids removed by filtration impurity, keep filtrate;
(4), decolorizing column decolouring, above-mentioned filtrate is by decolorizing column, sloughs pigment, remaining protein and other impurity, obtains water white mixed nucleotides destainer;
(5), the sodium filter concentrates: above-mentioned mixed nucleotides destainer is by the sodium filter membrane, and the impurity of filtering portion water and small molecular weight obtains concentrated solution and is the mixed nucleotides product.
If target product is a solid, then the concentrated solution of above-mentioned steps (5) gained can further spray-dried acquisition mixed nucleotides powder.
The present invention compares with the method that obtains mixed nucleotides that is mixed by a certain percentage with mononucleotide, because the process of manufacture order Nucleotide except the needs removal of impurity, also will be passed through chromatography with the enzymolysis solution of Yeast Nucleic Acid, is separated into the solution of four kinds of mononucleotides; Concentrate respectively then, crystallization, drying, this is a very long and complicated process.By comparison, technical scheme of the present invention has following advantage:
Production cycle is about 1/3rd of mononucleotide technology; Energy consumption is about 1/2nd of mononucleotide technology; Blowdown is about 1/2nd of mononucleotide technology; Security is good; Product yield increases substantially; Production cost descends significantly; The investment of founding the factory also descends significantly.
Embodiment
Below the specific embodiment of the present invention is described:
Production method according to the mixed nucleotides of present embodiment may further comprise the steps successively:
(1), preparation rna solution: Yeast Nucleic Acid is added in the entry, add alkali simultaneously, make the Yeast Nucleic Acid dissolving;
(2), enzymolysis Yeast Nucleic Acid: described rna solution is transferred to about pH7, about 70 ℃, add 5 '-phosphodiesterase, Yeast Nucleic Acid is broken down into 5 '-adenylic acid (AMP), 5 '-cytidylic acid, 5 '-uridylic acid and 5-guanylic acid, obtains the mixing solutions of Nucleotide;
(3), solids removed by filtration impurity, keep filtrate;
(4), decolorizing column decolouring, above-mentioned filtrate is by being equipped with the decolorizing column of acid decolorizing resin or granulated active carbon, sloughs pigment, remaining protein and other impurity, obtains water white mixed nucleotides destainer;
(5), the sodium filter concentrates: above-mentioned mixed nucleotides destainer is the sodium filter membrane of 2 nanometers by mean pore size, and the impurity of filtering portion water and small molecular weight obtains concentrated solution and is the mixed nucleotides product;
(6), spraying drying: the further spray-dried acquisition mixed nucleotides powder of concentrated solution.
At first under the prerequisite of strictness control enzymolysis liquid of ribonuclease quality, remove solid impurity after filtration, obtain limpid, transparent but with the enzymolysis solution of raw material Yeast Nucleic Acid inherent pigment; This enzymolysis solution is through decolorizing column, sloughs the pigment that brought by raw material Yeast Nucleic Acid and the protein and some impurity of solubilised state, and the quality of enzymolysis filtrate further promotes, and obtains being water white destainer; Destainer is that the sodium filter membrane of 2 nanometers is removed small molecular weight impurity remaining in portion water and the destainer by mean pore size again.In the spissated while, solution is further purified.
By above quality control process, at the character of the impurity that may exist in the enzymolysis solution, adopt various mode progressively to remove impurity, obtain high-quality sodium filter concentration liquid; Spray-dried again, just obtain high-quality mixed nucleotides powder, the technology of production Nucleotide of the present invention, Nucleotide can be used as nutritious prod.This operational path is short, the yield height, and cost is low, and blowdown is few; The product content height, color is white, and quality is good, and the ratio of four kinds of mononucleotides is the ratio in the natural nucleotide, is the most rational, thus for formulating the quality standard of mixed nucleotides, condition has been created in standard nucleotide nutrient product market.
Claims (2)
1, a kind of production method of mixed nucleotides is characterized in that: may further comprise the steps successively:
(1), preparation rna solution: Yeast Nucleic Acid is added in the entry, add alkali simultaneously, make the Yeast Nucleic Acid dissolving;
(2), enzymolysis Yeast Nucleic Acid: described rna solution is transferred to neutrality, add 5 '-phosphodiesterase, Yeast Nucleic Acid is broken down into 5 '-adenylic acid (AMP), 5 '-cytidylic acid, 5 '-uridylic acid and 5-guanylic acid, obtains the mixing solutions of Nucleotide;
(3), solids removed by filtration impurity, keep filtrate;
(4), decolorizing column decolouring: above-mentioned filtrate is by decolorizing column, sloughs pigment, remaining protein and other impurity, obtains water white mixed nucleotides destainer;
(5), the sodium filter concentrates: above-mentioned mixed nucleotides destainer is by the sodium filter membrane, and the impurity of filtering portion water and small molecular weight obtains concentrated solution and is the mixed nucleotides product.
2, the production method of a kind of mixed nucleotides according to claim 1 is characterized in that: this production method also comprises step (6): by the spray-dried mixed nucleotides powder that makes of above-mentioned steps (5) gained concentrated solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101554841A CN101376906A (en) | 2008-10-06 | 2008-10-06 | Production method for mixed nucleotides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101554841A CN101376906A (en) | 2008-10-06 | 2008-10-06 | Production method for mixed nucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101376906A true CN101376906A (en) | 2009-03-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008101554841A Pending CN101376906A (en) | 2008-10-06 | 2008-10-06 | Production method for mixed nucleotides |
Country Status (1)
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| CN (1) | CN101376906A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103331035A (en) * | 2013-06-28 | 2013-10-02 | 南京工业大学 | Method for decolorizing nucleotide enzymatic hydrolysate |
| CN107529809A (en) * | 2015-03-06 | 2018-01-02 | 内蒙古伊利实业集团股份有限公司 | Polynucleotide composition and its application in food |
-
2008
- 2008-10-06 CN CNA2008101554841A patent/CN101376906A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103331035A (en) * | 2013-06-28 | 2013-10-02 | 南京工业大学 | Method for decolorizing nucleotide enzymatic hydrolysate |
| CN107529809A (en) * | 2015-03-06 | 2018-01-02 | 内蒙古伊利实业集团股份有限公司 | Polynucleotide composition and its application in food |
| CN107529809B (en) * | 2015-03-06 | 2020-03-13 | 内蒙古伊利实业集团股份有限公司 | Nucleotide composition and application thereof in food |
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Application publication date: 20090304 |