CN101372515A - Polymer and use thereof - Google Patents
Polymer and use thereof Download PDFInfo
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- CN101372515A CN101372515A CNA2008100549297A CN200810054929A CN101372515A CN 101372515 A CN101372515 A CN 101372515A CN A2008100549297 A CNA2008100549297 A CN A2008100549297A CN 200810054929 A CN200810054929 A CN 200810054929A CN 101372515 A CN101372515 A CN 101372515A
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Abstract
The invention belongs to the field of energy technology and food technology, and more particularly relates to a polymer and the application thereof. The polymer is composed of 34.8% of sugar, 63.1% of fat and 1.5% of polypeptide; wherein, the sugar component contains glucose, rhamnose, seminose and glucuronic acid; fat component contains 6.2% of hexadecanoic acid methyl ester and 93.8% of methyl stearate; the polypeptide component respectively contains 11.6% of asparticacid, 6.1% of threonine, 4.8% of serine, 11.7% of glutamic acid, 6.7% of glycin, 9.8% of lactamine, 6.7% of valine, 1.8% of methionine , 4.6% of isoleucine, 9.8% of leucine, 3.4% of tyrosine, 5.9% of phenyl alanine, 3.1% of lysine, 1.3% of histidine, 7.6% of arginine and 5.2% of proline. Sanzanjiao can be taken as drilling mud conditioner and jelly glue polymer used for water shutoff profile control treatment in oil field to be applied to petroleum extraction, and the characteristics of jelling and thickening lead the polymer to be taken as food additive to be widely applied to the food production field.
Description
Affiliated technical field
The invention belongs to energy technology and food technology field, specifically, it relates to a kind of polymkeric substance---and three praise glue and application thereof.
Background technology
Many microorganisms of occurring in nature all have the ability of synthetic polymer, and these polymkeric substance stick to cell surface or are secreted in the nutrient solution with unsetting mucus shape, and microorganism is played a protective role.The polymkeric substance of microorganisms is except the biological significance to himself, because it has safety non-toxic, physico-chemical property uniqueness, with short production cycle, the restriction that is not subjected to season and geographical environmental condition, has the stronger market competitiveness and vast potential for future development.Some polymkeric substance have become important biological technology products, and for example xanthan gum, gelling gum, fertile logical sequence glue etc. are widely used in a plurality of fields such as oil, chemical industry, pharmacy and food as thickening material, stablizer, emulsifying agent and jelling agent.
In field of petroleum exploitation, the polymer mud treatment agent is the important outer adding assistant of oil field operation, and its effect not only can be carried to ground with drilling cuttings, and can fall low friction resistance, improves drilling speed and effectively suppresses dehydration, improves the well cementation rate.Chemical slurry treatment agents such as polyacrylamide, polyvinyl alcohol and sulfomethylation multipolymer widely-used brings extremely serious environmental pollution problem, and have polysaccharide polymers such as thickening and pseudoplastic xanthan gum, not only environmental protection, and its effect that is used for treating agent of drilling mud also is better than chemical slurry.In order to improve the recovery ratio in oil field, in handling, Water in Water Shutoff in Oil Field, profile control also often use the frozen glue base polymer, this base polymer can rely on its physics obstruction, capture effect and the absorption of blocking agent in the stratum to improve flow resistance, thereby improve the ununiformity of water injection well suction, improve water injection well sweep efficiency longitudinally, increase the workable reserve in oil field.Polymkeric substance has better tackifying, salt resistance, shear resistant, not only the aqueous solution has pseudo-plasticity, and the frozen glue body after crosslinked also has good pseudo-plasticity, be the desirable blocking agent that is used for the earth formation deep profile control, the polymkeric substance that uses as water plugging profile control agent mainly contains polysaccharide G and xanthan gum etc. at present.Field test is the result show, the polymkeric substance profile control is more more effective than injecting synthetic organic polymer or gel, does not have influence to follow-up water drive, to oily water separation and oil property, economy, environmental protection, equipment is simple, easy construction, and validity period is long, decreasing water cut and increasing oil is effective.
Characteristics such as the thickening of polymkeric substance, gelling, stable foam, emulsification and dispersion make it can control moisture content in the food effectively, improve outward appearance, shape, matter structure feature, mouthfeel and the local flavor etc. of meat product, milk-product, candy, cake.In addition, polymkeric substance has biocompatibility, security and advantage low in calories, thereby is widely used in producing and developing various functional health food.
Along with to polymers function and active understanding, its related development technology is by people's pay attention to day by day.The subject matter that polymkeric substance exploitation at present faces is: fermentation production rate and sugared transformation efficiency are lower, and the alcohol precipitation extraction process need consume a large amount of alcohol.Therefore develop the new polymers of high yield, reduce production costs, expanding its Application Areas becomes matter of utmost importance.At present, the production of domestic new polymers and use at the early-stagely awaits further research and development.
Summary of the invention
The objective of the invention is to development and application problem, provide a kind of polymkeric substance-three to praise glue, and the application of this polymkeric substance at oil and field of food is provided at the microorganism synthetic polymer.
Overall technology design of the present invention is:
A kind of polymkeric substance---three praise glue, and this polymkeric substance is the polymkeric substance that is made of the sugar of following percentage composition, fat, polypeptide, and wherein sugar 34.8%, fat 63.1%, polypeptide 1.5% contain glucose, rhamnosyl, seminose and glucuronic acid in the sugar component; The ester class component that comprises following percentage composition in the fat component, wherein methyl palmitate 6.2%, Methyl Stearate 93.8%; The amino acid that contains following percentage composition in the polypeptide fraction respectively, wherein Aspartic Acid 11.6%, Threonine 6.1%, Serine 4.8%, L-glutamic acid 11.7%, glycine 6.7%, L-Ala 9.8%, Xie Ansuan 6.7%, methionine(Met) 1.8%, Isoleucine 4.6%, leucine 9.8%, tyrosine 3.4%, phenylalanine 5.9%, Methionin 3.1%, Histidine 1.3%, arginine 7.6%, proline(Pro) 5.2%.Because wherein acidic amino acid-aspartic acid and L-glutamic acid proportion are the highest, reach 23.3%, therefore regulate to make three to praise the glue precipitation about pH to 3.0.
This polymkeric substance---three praise glue is applied to drilling well, oil production and foodstuff production field.
The concrete physicochemical characteristics of this polymkeric substance also has among the present invention:
The weight-average molecular weight of this polymkeric substance (Mw) is 408KDa, and number-average molecular weight (Mn) is 382KDa, and molecular weight distribution (Mw/Mn) is 1.07, belongs to single dispersed substance.
This polymkeric substance is made up of the elementary composition of following weight percentage: carbon 30.38%, nitrogen element 0.48%, protium 6.12%, oxygen element 63.02%.
The infrared scan of this polymkeric substance is characterized as: at 3441cm
-1Near the strong absorption peak of hydroxyl O-H stretching vibration, 2934cm are arranged
-1The stretching vibration of C-H is arranged, 1604cm
-1Near the N-H angle vibration of one-level amino is arranged; 1414cm
-1Near the stretching vibration of carboxyl C-O, 1400-1200cm are arranged
-1The angle vibration absorption peak that the CH of carboxyl is arranged, 1081cm
-1Near the stretching vibration of C-O, 1037cm are arranged
-1Near O-H angle vibration, 904cm are arranged
-1Near the asymmetric stretching vibration of pyranoid ring is arranged.
This polymkeric substance also can be produced or the synthetic acquisition of chemical process by plant for by deposit number being Sphingol single-cell (Sphingomonassp.) generation of CGMCC No.1650.
The aqueous solution of this polymkeric substance has very high viscosity, and presents typical false creeping bulk properties, and the viscosity of polymers soln do not change with minute length, does not belong to thixotropic fluid; As jelling agent, the polymers soln with 〉=0.6% is heated to 75 ℃, can form gel after being cooled to room temperature; At Ca
2+Under the condition that exists, 〉=0.2% polymers soln just can form gel, and with the raising of polymer concentration, gel hardness obviously increases; Aspect emulsifying activity, 〉=0.2% polymkeric substance promptly can be the complete emulsification of 0# diesel oil in 12h.
Provided by the invention three aqueous solution of praising glue have tangible viscosity, viscosimetric analysis standard according to xanthan gum, 1% three to praise glue KCl solution viscosity under the condition of 6r/min be 13160mPas, the viscosity of xanthan gum is 12180mPas under the same terms, illustrates that three praise glue and have good thickening effectiveness.Praise the aqueous solution that glue is mixed with the different concns gradient with three, its rheological properties is: three viscosity of praising sol solution increase with the increase of its concentration, viscosity amplification is especially remarkable during higher concentration, and certain density three praise sol solution under constant rotational speed, viscosity does not change with minute length, therefore promptly not free dependency does not belong to thixotropic fluid.Three praise glue solution viscosity reduces along with the increase of shearing rate, after rotating speed reduces, viscosity increases again gradually, state when returning to the test beginning at last, curve is the line of delimitation in the maximum speed of x axle, the right and left symmetry shows that this soltion viscosity does not have hysteresis quality when recovering, and three praise glue solution belongs to typical false creeping body.
With 0.6% three praise sol solution and be heated to 75 ℃, can form gel after being cooled to room temperature.Add Ca
2+After, with 0.2% three praise sol solution and be heated to 75 ℃ and kept 10 minutes, the cooling back becomes gel, and with three raisings of praising gum concentration, gel hardness obviously increases.Along with Ca
2+The increase of concentration, gel hardness remain unchanged after increasing earlier substantially; Different three praise gum concentration, required Ca when reaching the highest hardness value
2+The add-on difference, three to praise gum concentration high more, required Ca
2+Amount big more, be specially: three praise gum concentration 0.2%～0.4%, required Ca
2+10～15mmol/L; Three praise gum concentration 0.6%～1.2%, required Ca
2+20mmol/L～25mmol/L; Adding excess amount of ions (〉 25mmol/L) gel hardness descends to some extent.
Three praise the emulsifying activity of glue: three of different concns is praised glue solution mix with 0# diesel oil, after vortex vibration 2min leaves standstill 24h, can be observed obviously stable emulsion layer, and with three increases of praising gum concentration, emulsifying property improves, the EI of 0.1% polymkeric substance
24Be the EI of 72%, 0.2% polymkeric substance
24Reach 100%.Illustrate that three praise glue and have the good emulsifying performance, can be with the complete emulsification of petroleum hydrocarbon, and form stable milk sap.
Substantial advantage that the present invention is obtained and significant technical progress are:
Provided by the invention three praise glue be a kind of by glycolipidpeptide in conjunction with the polymer new polymers that constitutes, because acidic amino acid-aspartic acid and L-glutamic acid proportion are the highest in the polypeptide portion, therefore reach 23.3%, regulate about the pH to 3.0 of fermented liquid to make three to praise the glue precipitation.Three aqueous solution of praising glue have very high viscosity, its viscosity increases with three increases of praising gum concentration, reduce along with the increase of shearing rate, when shearing rate increases once more, three viscosity recoveries of praising glue do not have hysteresis quality, present typical false creeping bulk properties, and three viscosity of praising sol solution do not change with minute length, do not belong to thixotropic fluid.As jelling agent, at 10mmol/L Ca
2+Under the condition that exists, 0.2% three praise sol solution and just can form gel, and with the raising of polymer concentration, gel hardness obviously increases.Aspect emulsifying activity, 0.2% three praise glue promptly can be the complete emulsification of 0# diesel oil.Three to praise glue be a kind of new polymers with thickening property, pseudo-plasticity, one-tenth gel property and emulsifying activity, be applied to oil production with the frozen glue base polymer and obtain good effect as treating agent of drilling mud and Water in Water Shutoff in Oil Field, profile control processing, its gelling and thickening characteristic can also make it be widely used in the foodstuff production field as foodstuff additive.
Description of drawings
Fig. 1 three praises the infrared absorption pattern of glue.
Fig. 2 three praises the qualitative colour developing figure of chemical composition of glue.
Fig. 3 is the molecular weight that gel permeation chromatography-multi-angle laser light scattering coupling technique is measured polymkeric substance.
Fig. 4 is the monose composition that gc analysis three is praised glue.
Fig. 5 is the lipid acid composition that analytical reagent composition three is praised glue.
Fig. 6 three praises the relation curve of gum concentration and soltion viscosity.
Fig. 7 shears in the rotating speed loopback test the three viscosity change curves of praising glue.
Fig. 8 is a temperature variation to three influences of praising sol solution viscosity.
Fig. 9 is that pH changes the influence of praising sol solution viscosity to three.
Figure 10 is that 0.8% polymers soln is heated to 75 ℃ and becomes gel figure after being cooled to room temperature.
Figure 11 three praises the design sketch of latex diesel oil.
The glycolipidpeptide colour developing is respectively glucose, Yelkin TTS and leucine with positive control among Fig. 2, retention time 12.389,14.238,14.542 and 16.032 component are followed successively by rhamnosyl, seminose, glucose and interior mark inositol six acetic ester among Fig. 4, A is a methyl palmitate among the figure, and B is a Methyl Stearate.
Embodiment
Three preparation process and the processing parameters of praising glue are in the present embodiment:
Utilize Sphingol single-cell (Sphingomonas sp.CGMCC No.1650) fermentative preparation to obtain, fermention medium is: glucose 40g/L, peptone 2.5g/L, yeast powder 0.15g/L, K
2HPO
41.6g/L, NaCl 0.5g/L, MgSO
40.2g/L, MnCl
20.004g/L, FeSO
40.005g/L, CaCO
31.0g/L, pH7.0.Make seed liquor after the slant strains activation, the bottled 100mL substratum of 250mL triangle, sterilization and inoculation are placed on the rotary shaking table, and 180r/min cultivated 72 hours for 30 ℃.In addition, this polymkeric substance also can obtain by plant or so that the method for chemosynthesis is synthetic.
Three purification step and the processing parameters of praising glue are in the present embodiment:.
In 100 ℃ of heating 30 minutes, centrifugal 45 minutes of 10000r/min removed precipitation with fermented liquid, collected supernatant liquor and in the centrifugal 30min of 10000r/min, so repeated 3～4 times, did not have somatic cells to microscopy.Merge supernatant liquor, add the chloroform of isopyknic 5:1: the propyl carbinol mixture, thermal agitation 30 minutes, centrifugal 20 minutes of 8000r/min removes protein impurity.Add about 1mol/L salt acid for adjusting pH value to 3.0 to aqueous phase, make three to praise the glue precipitation, in deionized water, dialyse to pH neutrality with the dialysis tubing of holding back scope 8000-15000, cross the sephacryl s-400 (gel chromatographic columns of 2.6com * 40cm), the deionized water wash-out, anthrone-sulfuric acid process spike detects, and collects main peak, merge behind the chromatography repeatedly and collect liquid, obtain three after the dialysis freeze-drying and praise the pure product of glue.
Present embodiment provides three qualitative analyses of praising glue.
Three praise the hydrolysis of glue: get 5mg three and praise the pure product of glue, add 2mol/L sulfuric acid, put 120 ℃ of tube sealing hydrolysis 12h, with the barium carbonate neutralization, the centrifuging and taking supernatant is standby.Get 0.5% 3 and praise glue solution and each 5 microlitre of hydrolyzation sample, point sample on silica-gel plate dries.Sugar developer qualitatively is sulfuric acid-anthrone solution (2% anthrone, 85% vitriol oil), 110 ℃ of baking oven for heating 5～10min colour developings; With 0.1% ninhydrin solution quick humidification, put into 110 ℃ of baking ovens and heat 10～15min colour developing when peptide is qualitative; Fat qualitative in ammonium molybdate-perchloric acid solution quick humidification, in 105 ℃ of heating 20min colour developings.Before and after the acid hydrolysis three praised and all is tangible blue-greenish colour spot after the glue sample develops the color with sulfuric acid-anthrone, contains carbohydrate content in the interpret sample; Three praise the glue sample is light blue spot with the colour developing of ammonium molybdate-perchloric acid, and the sample colour developing after the acidolysis is more obvious, illustrates that three praise and contain lipid material in the glue; Praise the glue sample and do not have obvious spot with the triketohydrindene hydrate not hydrolysis three that develops the color, but three after the hydrolysis praise the glue sample and be purple yellow, illustrate that three praise and contain polypeptide fraction (Fig. 2) in the glue.
What present embodiment provided three praises the xanthan molecule flow measurement.
Measure the molecular weight of polymkeric substance with gel permeation chromatography (GPC)-multi-angle laser light scattering (MALLS) coupling technique.Use instrument to be Agilent 1100 liquid chromatographies, the strain of TSK4000 chromatogram, Wyatt OPTIL differential refraction detector, Dawn EOS ten anistree laser light scattering detectors, and dn/dc pH-value determination pH Optilab DSP differential refractometer.The mensuration damping fluid is a ultrapure water, flow velocity 0.5ml/min, sample concentration 1mg/ml, sample size 100ml.Carrying out data acquisition and processing (DAP) with the special-purpose ASTRA software of SEC-MALLS calculates.The GPC-MALLS coupling technique determines the absolute molecular weight and the molecular weight distribution of polymkeric substance, the polymer samples of purifying only has one symmetric unimodal (Fig. 3), the refractive index increment dn/dc value that Optilab DSP differential refractometer is measured polymkeric substance is 0.135, the weight-average molecular weight (Mw) that the ASTRA computed in software goes out polymkeric substance is 408KDa, number-average molecular weight (Mn) is 382KDa, molecular weight distribution (Mw/Mn) is 1.07, and Mw/Mn belongs to single dispersed sample in 1.02～1.10 scopes.
What present embodiment provided three praises the glue proximate analysis.
Adopting phenolsulfuric acid method (Zhang Weijie etc. 1987) to measure the three sugared content of praising glue is 34.8%, accounts for 20.4% with glucuronic acid in sulfuric acid-carbazole method mensuration sugar component.Get three and praise glue sample 5mg, after adding the 2mol/L trifluoroacetic acid, put 120 ℃ of tube sealing hydrolysis 8h, be evaporated to dried, add mark inositol six acetic ester in 10 milligrams of oxammonium hydrochlorides and 7 milligrams, add 0.5 milliliter of pyridine again, put into 90 degrees centigrade of water-bath reacting by heating 30 minutes and vibration, take out postcooling to room temperature; Add 0.5 ml acetic anhydride, continue reaction at 90 degrees centigrade and carried out acetylize in 30 minutes, reaction product is analyzed with Agilent 6820 gas chromatography systems, and chromatographic column is SPB
TM-5 capillary column (30m * 0.53mm i.d., 15 μ mthickness, Supelco).Chromatographic program is as follows:
Injection port: 280 ℃;
Column temperature: 120 ℃,
5min;
Detector (FID) temperature: 280 ℃;
Carrier gas (N
2) 35mL/min;
Air 400mL/min;
Hydrogen (H
2) 30mL/min;
Tail blows (N
2) 20mL/min.
According to various standard monose retention time determine that relatively three praise the monosaccharide component (Fig. 4) of glue, relative retention time is that 16.032 peak is interior mark inositol six acetic ester, retention time 12.389,14.238 and 14.542 component be followed successively by rhamnosyl,
Seminose and glucose, according to each component peaks area, the ratio of being calculated glucose, rhamnosyl, seminose in the sample as can be known by marker method is 6:1.5:1.
2 grams three are praised the glue sample place the hydrolysis pipe, add the hydrochloric acid of 5mL 6mol/L, vacuumize tube sealing, at 110 ℃ of hydrolysis 24h, get the 0.5mL evaporate to dryness, add the dissolving with hydrochloric acid of 0.02mol/L, carry out amino acid composition analysis with HITACHI L-8800 type automatic analyzer for amino acids.
With chloroform/methanol method (Zhai Yongxin, 1991) measuring three lipid contents of praising glue is 63.1%, polymer samples is (SO CM after esterification, 1999) carrying out GC-MS analyzes, mass spectrum condition: HP G1800A type mass spectrograph, HP-5 capillary chromatographic column, electron-bombardment (EI) ion source, ionizing energy 70Ev, sweep limit m/z 10～400.The result shows that (Fig. 5) exists methyl palmitate and Methyl Stearate.The lipid that polymkeric substance is described consists of 16 carbon fatty acids and 18 carbon fatty acids, draws both shared ratios according to calculated by peak area and is respectively 6.2% and 93.8%.
Three viscosimetric analysis and the rheological properties of praising glue that present embodiment provides.
Three praise the preparation of sol solution: take by weighing three of certain mass and praise the rubber powder end, be dissolved in the proper amount of deionized water, stir and to make it to be uniformly dispersed, 4 ℃ of swellings are spent the night, and it is to be measured to return to room temperature.Sol solution is praised in three of preparation 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1.0% and 1.2%, adopt No. 4 rotor, rotating speed 250r/min, measure soltion viscosity under the room temperature, illustrate that three viscosity of praising sol solution increase with three increases of praising gum concentration, viscosity amplification especially significantly (Fig. 6) during higher concentration.
The rotating speed loopback test: sol solution is praised in three of preparation 0.5%, use DV-II+PRO control software RHEOCALC editor, adopt No. 4 rotor, set rotating speed and increase continuously 250r/min from 5r/min, be reduced to 5r/min continuously from 250r/min immediately afterwards, viscometric changing value under the room temperature.Certain density three praise sol solution under constant rotational speed, and viscosity does not change with minute length, and promptly not free dependency does not belong to thixotropic fluid.Three praise sol solution viscosity increases with rotating speed and reduces, behind the 60r/min downtrending mild, illustrate that this solution has the shear thinning performance, be typical pseudoplastic fluid; In the rotating speed reduction process, viscosity increases gradually, returns to the state in when beginning test at last, and as the line of delimitation, the right and left symmetry shows that this soltion viscosity does not have hysteresis quality (Fig. 7) when recovering to curve in the vertical line of x axle 100r/min.
Temperature variation is to three influences of praising sol solution viscosity: sol solution is praised in three of preparation 1.0%.Use DV-II+PRO control software RHEOCALC editor, adopts No. 5 rotor, rotating speed 250r/min, three viscosity numbers (Fig. 8) of praising sol solution when the mensuration temperature is 20 ℃, 30 ℃, 40 ℃, 50 ℃, 60 ℃, 70 ℃ and 80 ℃.Under less than 70 ℃ of conditions, soltion viscosity changes little; Be warming up to 80 ℃, viscosity slightly reduces, and shows that three praise glue and have good temperature tolerance.
The pH value changes the influence of praising sol solution viscosity to three: sol solution is praised in three of preparation 0.1%.With HCl or NaOH regulator solution pH be 4,5,6,7,8,9, and 10, adopts No. 2 rotors, rotating speed 250r/min, the viscosity number of mensuration solution under the room temperature.Under the high alkalinity condition, soltion viscosity slightly increases from pH4.0, and it is little to show that the above acid-basicity of pH4 is praised the sol solution viscosity influence to three, and three praise glue has good resistance to acids and bases (Fig. 9).
Different metal ion pair three is praised the influence of sol solution viscosity: sol solution is praised in preparation three, take by weighing an amount of medicine, be dissolved in the deionized water and stirring, treat that it fully dissolves back and three and praises sol solution and mix (final concentration: polymkeric substance 0.25%, ion 0.1mol/L), with magnetic stirrer evenly and leave standstill 4～6h, make the abundant balance of solution.Adopt No. 3 rotor, rotating speed 250r/min, the viscosity number of mensuration solution under room temperature.It is not obvious that sodium, potassium, magnesium, calcium, zinc, copper, violent, cobalt and ferrous ion are praised the viscosity influence of sol solution to three, 1% three to praise the viscosity of glue in 10%KCl solution be 1520mPas, and the viscosity in 1% KCl solution is 1538mPas, show that this polymkeric substance has good salt tolerance, can be in the higher field use of salinity.
The three one-tenth gel propertys of praising glue that present embodiment provides.
Three praise sol solution except having viscosity higher, are heated to be cooled to room temperature more than 75 ℃ and also can to form gel (Figure 10), under the condition that does not add metal ion, form the required polymer concentration of gel 〉=0.6%; Add metal ion, 0.2% polymkeric substance can form gel.Common monovalent ion such as K
+, Na
+, divalent ion removes Cu
2+, Fe
2+Outward, Ca
2+, Mg
2+, Zn
2+, Mn
2+All can make three to praise sol solution formation gel; Trivalent ion such as Fe
3+And Al
3+, can make three to praise glue formation lumps precipitation.No matter whether add ion, formed gel is the thermal reversibility gel.
Three emulsifying activities of praising glue that present embodiment provides.
Get 10 milliliters scale test tube, add three of 6 milliliters of 0# diesel oil and 4 milliliters of different concns and praise glue solution, mixing and thermal agitation 2 minutes, room temperature left standstill after 24 hours measures the emulsification index, divided by total height, multiply by 100% again, i.e. EI with the height of emulsion layer
24Value (Figure 11).After leaving standstill 24h, can be observed obviously stable emulsion layer, and with three increases of praising gum concentration, emulsifying property improves, 0.1% 3 praises the EI of glue
24Be 72%, 0.2% three to praise the EI of glue
24Reach 100%, EI is described
24Have the good emulsifying performance, can be with the complete emulsification of petroleum hydrocarbon, and form stable milk sap.Under identical experiment condition, water and 0# diesel oil system vortex vibration 2min only leave standstill 2min and just return to initial oily water separation state.
Claims (7)
1. polymkeric substance, it is characterized in that: this polymkeric substance is the polymkeric substance that is made of the sugar of following percentage composition, fat, polypeptide, wherein sugar 34.8%, fat 63.1%, polypeptide 1.5% contain glucose, rhamnosyl, seminose and glucuronic acid in the sugar component; The ester class component that comprises following percentage composition in the fat component, wherein methyl palmitate 6.2%, Methyl Stearate 93.8%; The amino acid that contains following percentage composition in the polypeptide fraction respectively, wherein Aspartic Acid 11.6%, Threonine 6.1%, Serine 4.8%, L-glutamic acid 11.7%, glycine 6.7%, L-Ala 9.8%, Xie Ansuan 6.7%, methionine(Met) 1.8%, Isoleucine 4.6%, leucine 9.8%, tyrosine 3.4%, phenylalanine 5.9%, Methionin 3.1%, Histidine 1.3%, arginine 7.6%, proline(Pro) 5.2%.
2. polymkeric substance according to claim 1 is characterized in that: the weight-average molecular weight of this polymkeric substance (Mw) is 408KDa, and number-average molecular weight (Mn) is 382KDa, and molecular weight distribution (Mw/Mn) is 1.07.
3. polymkeric substance according to claim 1 is characterized in that this polymkeric substance is made up of the elementary composition of following weight percentage: carbon 30.38%, nitrogen element 0.48%, protium 6.12%, oxygen element 63.02%.
4. polymkeric substance according to claim 1 is characterized in that the infrared scan of this polymkeric substance is characterized as: at 3441cm
-1Near the strong absorption peak of hydroxyl O-H stretching vibration, 2934cm are arranged
-1The stretching vibration of C-H is arranged, 1604cm
-1Near the N-H angle vibration of one-level amino is arranged; 1414cm
-1Near the stretching vibration of carboxyl C-O is arranged, 1400
-1200cm
-1The angle vibration absorption peak that the CH of carboxyl is arranged, 1081cm
-1Near the stretching vibration of C-O, 1037cm are arranged
-1Near O-H angle vibration, 904cm are arranged
-1Near the asymmetric stretching vibration of pyranoid ring is arranged.
5. polymkeric substance according to claim 1 is characterized in that this polymkeric substance for by deposit number being Sphingol single-cell (Sphingomonas sp.) generation of CGMCC No.1650, also can be produced or the synthetic acquisition of chemical process by plant.
6. polymkeric substance according to claim 1 is characterized in that the aqueous solution has very high viscosity, and presents typical false creeping bulk properties, and the viscosity of polymers soln do not change with minute length, does not belong to thixotropic fluid; As jelling agent, the polymers soln with 〉=0.6% is heated to 75 ℃, can form gel after being cooled to room temperature; At Ca
2+Under the condition that exists, 〉=0.2% polymers soln just can form gel, and with the raising of polymer concentration, gel hardness obviously increases; Aspect emulsifying activity, 〉=0.2% polymkeric substance promptly can be the complete emulsification of 0# diesel oil in 12h.
7. according to the application of claim 1,2,3,4,5 or 6 described polymkeric substance, it is characterized in that being used for drilling well, oil production and foodstuff production field.
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| CN104770470A (en) * | 2015-04-22 | 2015-07-15 | 山东洁晶集团股份有限公司 | Thickening agent for yoghourt |
| CN104789195A (en) * | 2015-02-06 | 2015-07-22 | 中国石油大学(北京) | Bionic shale inhibitor and preparation method thereof and drilling fluid and application thereof |
| CN106467562A (en) * | 2015-08-14 | 2017-03-01 | 中石化石油工程技术服务有限公司 | A kind of aminoacid sugar ester, its preparation method and its application |
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| CN104789195A (en) * | 2015-02-06 | 2015-07-22 | 中国石油大学(北京) | Bionic shale inhibitor and preparation method thereof and drilling fluid and application thereof |
| CN104789195B (en) * | 2015-02-06 | 2016-02-03 | 中国石油大学(北京) | Bionical shale control agent and preparation method thereof and drilling fluid and application thereof |
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| CN108675857B (en) * | 2018-05-31 | 2021-05-25 | 天津农学院 | Application of sanzan glue in slow and controlled release fertilizer, film material of slow and controlled release fertilizer, coated slow and controlled release fertilizer based on sanzan glue and preparation method of coated slow and controlled release fertilizer |
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| CN108719730A (en) * | 2018-06-07 | 2018-11-02 | 天津农学院 | Three suspension stabilizers and suspending beverage praised application of the glue in suspension stabilizer, be used to prepare suspending beverage |
| CN108719730B (en) * | 2018-06-07 | 2022-01-04 | 天津农学院 | Application of sanzan gum in suspension stabilizer, suspension stabilizer for preparing suspension beverage and suspension beverage |
| CN109875028A (en) * | 2019-04-09 | 2019-06-14 | 北京美添前景科技有限公司 | It is a kind of improve meat products texture and emulsification property compound thickener and its preparation method and application |
| CN111057711A (en) * | 2019-12-25 | 2020-04-24 | 廊坊梅花生物技术开发有限公司 | Sphingomonas engineering bacteria and construction method and application thereof |
| CN111057711B (en) * | 2019-12-25 | 2022-01-18 | 廊坊梅花生物技术开发有限公司 | Sphingomonas engineering bacteria and construction method and application thereof |
| CN112494431A (en) * | 2020-11-04 | 2021-03-16 | 天津农学院 | Trizan glue-based microsphere, nutrition and/or drug delivery system, preparation method and application |
| CN112494431B (en) * | 2020-11-04 | 2022-06-03 | 天津农学院 | Microsphere based on sanzan glue |
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