CN101371876B - Method for extracting and preparing total polysaccharide of compound abnormal savda mature agent - Google Patents
Method for extracting and preparing total polysaccharide of compound abnormal savda mature agent Download PDFInfo
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- CN101371876B CN101371876B CN200810072943XA CN200810072943A CN101371876B CN 101371876 B CN101371876 B CN 101371876B CN 200810072943X A CN200810072943X A CN 200810072943XA CN 200810072943 A CN200810072943 A CN 200810072943A CN 101371876 B CN101371876 B CN 101371876B
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Abstract
The invention provides a method for extracting and preparing total polysaccharide of compound abnormal savda munziq; wherein, the compound abnormal savda munziq is prepared by ten traditional Chinese medicinal materials including microcos fruit, oxtongue, liquorice, capillaire and humifuse euphorbia by certain proportion; the medicinal materials are decocted, soaked, filtered, concentrated by decompressing and dried into solid extractum which is crushed into the dry powder. The dry powder is dissolved in hot water and filtered; the filtrate goes through macroporous resin and is eluted with water; the eluant is concentrated and decolored and purified by active carbon and filtered; the purified eluant is concentrated by decompressing; after cooling, the filtrate is added with ethanol, stirred and settled to form precipitate which is added with hot water, stirred and re-dissolved and the solution is then filtered when being hot; the filtrate is concentrated and added with ethanol after cooling for settlement, followed by the stirring and settlement of the filtrate, and the filtering; the obtained filtrate is dried and finally the total polysaccharide product is obtained with the purity of over 50percent. The extraction and preparation method is characterized by low cost, simplicity, practicality and safety, and the prepared total polysaccharide can be prepared into capsules, tablets, oral liquid, granules, medical potion, syrup and pills.
Description
Technical field
The present invention relates to the extraction preparation of total polysaccharides, the especially improvement of the raising of purity and method adapts to a kind of extraction preparation method of total polysaccharides of compound abnormal savda mature agent especially.
Background technology
Abnormal savda mature agent is to prepare in proportion with ten flavor medical materials such as fruit of Cordia dichotoma Forst. f., Folium Fumicis Dentati, Radix Glycyrrhizae, venushair fern, Herba Euphorbiae Humifusae, decoct, soak, filter, dilute the Uygur medicine compound preparation that forms, be mainly used in treatment by difficult miscellaneous diseases such as tumor that unusual black gallbladder cross-examination caused, diabetes, hypertension, coronary heart disease; The Therapeutic Principle of associated diseases at first uses abnormal savda mature agent to make to cause the unusual black gallbladder matter of disease to be stacked into intestinal mucosa, use unusual black gallbladder matter scavenger that the unusual black gallbladder matter that is stacked into intestinal mucosa is excreted then, thereby reach the purpose of treatment disease.This method is that Uygur medicine is exclusive, can't compare with other medical science.
In recent years medical research shows; abnormal savda mature agent has the removing reactive oxygen free radical; improve the body anti-oxidation function; the protection lymphocyte; mitochondrion and DNA oxidative damage; Antiradiation injury; antithrombotic forms; improve body's immunity; regulate body nerve-endocrine-immunological network function; suppress human liver cancer cell (HepG2); people official's neck cancer cell (Hela); the T lymphoma cell; breast cancer cell (Bcap-37); the human colon cancer cell external increments of cancerous tumor cell such as (Caco-2); inhibition is by 1, and the formation of the unusual crypts focus of the inductive rat colon of 2-Dimethylhydrazine (DMH) reverses functions such as people's hepatocarcinoma drug-resistant cell strain multidrug resistance.At present, the research emphasis of abnormal savda mature agent is the extraction of abnormal savda mature agent active component and the detection of biological activity and medical value thereof.
The patent of prescription composition of (patent No. is ZL02130082.8) compound abnormal savda mature agent that applicant of the present invention obtains and preparation method thereof, only the prescription composition of abnormal savda mature agent, the preparation method of water extract and the partial action mechanism of disease preventing and treating thereof are set forth, abnormal savda mature agent is not further extracted, separates, more do not relate to and from abnormal savda mature agent, extract total polysaccharides class composition etc.
The present invention is in the method for numerous extraction total polysaccharides materials; the technical scheme of the best is made up; and successfully extract and preparation; practical proof this method safe and simple; non-environmental-pollution; the abnormal savda mature agent total polysaccharides content height that makes is applicable to large-scale production, does not see domestic and international any report so far.
Summary of the invention
The objective of the invention is to, a kind of extraction preparation method of total polysaccharides of compound abnormal savda mature agent is provided, product purity is more than 50%, and the abnormal savda mature agent total polysaccharides that this method is extracted can be made into uses such as capsule, tablet, oral liquid, granule, decoction, syrup, pill.
Technical solution of the present invention is achieved in that a kind of extraction preparation method of total polysaccharides of compound abnormal savda mature agent, the dry powder of getting abnormal savda mature agent is an amount of, add 20～100 ℃ of water of 2～20 times of amounts, stir dissolving in 10～60 minutes, filtered while hot; Filtrate decompression concentrates, and disposes through macroporous adsorptive resins; With 3～18 times of water gaging eluting, collect eluent; Eluent filters through the activated carbon decolorizing purification; Filtrate decompression concentrates and is cooled to 10～25 ℃, adds 95% ethanol of 3～6 times of amounts, stirs 15～30 minutes, leaves standstill 12～48 hours precipitations under 3～10 ℃ of conditions, filters; Taking precipitate is an amount of, add 50～100 ℃ of water of 2～20 times of amounts, stir dissolving in 10～60 minutes, filtered while hot, filtrate decompression concentrates, be cooled to 10～25 ℃, add 95% ethanol of 3～6 times of amounts, stirred 15～30 minutes, under 3～10 ℃ of conditions, left standstill 12～48 hours, form precipitation, filter, obtain the precipitate of purification; Drying precipitate, pulverizing promptly obtain abnormal savda mature agent total polysaccharides finished product, and its purity is more than 50%.
The extraction preparation method of above-mentioned total polysaccharides, filtrate decompression is concentrated into relative density 0.99-1.25.
The extraction preparation method of above-mentioned total polysaccharides, the large aperture resin model of selection is AB-8, D-101, HPD-100, HPD-300 or HPD-500.
The extraction preparation method of above-mentioned total polysaccharides, the active carbon of decolouring purification is commercially available powdered activated carbon.
The preparation method of above-mentioned total polysaccharides adopts vacuum drying or lyophilization or spray drying when dry.
The finished product of described total polysaccharides can be made into capsule, tablet, oral liquid, granule, decoction, syrup, pill.
The total polysaccharides that the present invention acquires adopts phenol-concentrated sulphuric acid method to measure, and is reference with the glucose, and purity is more than 50%.
The present invention's ethnic drug that has drawn from, the raw material speciality, select best extracting method, realized first medical development, medical scope had both been expanded in the successful extraction and preparation of the contained total polysaccharides of compound abnormal savda mature agent, increased new product for the dimension medicine again, extract total polysaccharides purity height, cost is low, and applied range shows technological progress.
The specific embodiment
The present invention is described further with embodiment.
Embodiment 1
Get the dried powder 3kg of abnormal savda mature agent, add 15000mL90 ℃ of hot water, stir and it was fully dissolved in 30 minutes, filtered while hot, filtrate decompression is concentrated into 6000mL, through the AB-8 macroporous adsorptive resins, with the 30000mL water elution, collect water elution liquid, add 2% activated carbon decolorizing purification, filter, filtrate decompression concentrates 6000mL, and concentrated solution is cooled to 10 ℃, add 12000mL95% ethanol, stirred 10 minutes, under 4 ℃ of conditions, left standstill 24 hours, form precipitation, filter and obtain precipitate.Precipitate adds 5000ml90 ℃ of hot water, stirs dissolving in 30 minutes, and it is 1.21 that filtered while hot, filtrate decompression are concentrated into relative density, concentrated solution is cooled to 10 ℃, adds 95% ethanol of 2 times of amounts, stirs 10 minutes, under 4 ℃ of conditions, left standstill 24 hours, form precipitation, filter the precipitate that obtains purification.Precipitate lyophilization, pulverizing obtain the total polysaccharides product of abnormal savda mature agent, and surveying purity by colorimetry is 54.7%.
Embodiment 2:
Get the dried powder 3kg of compound abnormal savda mature agent, add 18000mL85 ℃ of hot water, stir and it was fully dissolved in 40 minutes, filtered while hot, filtrate decompression is concentrated into 6500mL, through the D-101 macroporous adsorptive resins, with the 40000mL water elution, collect water elution liquid, add 2% activated carbon decolorizing purification, filter, filtrate decompression concentrates 6500mL, concentrated solution is cooled to 10 ℃, add 12500mL95% ethanol, stirred 15 minutes, under 5 ℃ of conditions, left standstill 36 hours, form precipitation, filter and obtain precipitate.Precipitate adds 6000ml92 ℃ of hot water, stirs dissolving in 20 minutes, and filtered while hot, filtrate decompression are concentrated into relative density 1.19, concentrated solution is cooled to 4 ℃, adds 95% ethanol of 1.5 times of amounts, stirs 20 minutes, under 5 ℃ of conditions, left standstill 36 hours, form precipitation, filter the precipitate that obtains purification.Precipitate lyophilization, pulverizing obtain the total polysaccharides product of abnormal savda mature agent, are 50.8% by colorimetric method for determining purity.
Claims (5)
1. the extraction preparation method of the total polysaccharides of a compound abnormal savda mature agent, it is characterized in that: the dry powder of getting abnormal savda mature agent is an amount of, adds 20～100 ℃ of water of 2～20 times of amounts, stirs dissolving in 10～60 minutes, filtered while hot; Filtrate decompression concentrates, and disposes through macroporous adsorptive resins; With 3～18 times of water gaging eluting, collect eluent; Eluent filters through the activated carbon decolorizing purification; Filtrate decompression concentrates, and is cooled to 10～25 ℃, adds 95% ethanol of 3～6 times of amounts, stirs 15～30 minutes, leaves standstill 12～48 hours precipitations under 3～10 ℃ of conditions, filters; Taking precipitate is an amount of, add 50～100 ℃ of water of 2～20 times of amounts, stir dissolving in 10～60 minutes, filtered while hot, filtrate decompression concentrates, be cooled to 10～25 ℃, add 95% ethanol of 3～6 times of amounts, stirred 15～30 minutes, under 3～10 ℃ of conditions, left standstill 12～48 hours, form precipitation, filter the precipitate of purification; Drying precipitate, pulverizing promptly get abnormal savda mature agent total polysaccharides finished product, and its purity is more than 50%.
2. the extraction preparation method of total polysaccharides according to claim 1, it is characterized in that: the large aperture resin model of selection is AB-8, D-101, HPD-100, HPD-300 or HPD-500.
3. the extraction preparation method of total polysaccharides according to claim 1 is characterized in that: the active carbon of decolouring purification is commercially available powdered activated carbon.
4. the extraction preparation method of total polysaccharides according to claim 1 is characterized in that: adopt vacuum drying or lyophilization or spray drying when dry.
5. the extraction preparation method of total polysaccharides according to claim 1, it is characterized in that: it is characterized in that: finished product can be made into capsule, tablet, oral liquid, granule, decoction, syrup, pill.
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| CN200810072943XA CN101371876B (en) | 2008-08-22 | 2008-08-22 | Method for extracting and preparing total polysaccharide of compound abnormal savda mature agent |
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| CN200810072943XA CN101371876B (en) | 2008-08-22 | 2008-08-22 | Method for extracting and preparing total polysaccharide of compound abnormal savda mature agent |
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| CN101371876B true CN101371876B (en) | 2011-08-03 |
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| CN101757128B (en) * | 2009-06-05 | 2012-03-14 | 新疆医科大学 | Extraction and preparation method of total phenol of compound abnormal savda munziq |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1410085A (en) * | 2002-08-12 | 2003-04-16 | 哈木拉提·吾甫尔 | Ripening agent and eliminating agent for abnormal black balliary matter and its preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1410085A (en) * | 2002-08-12 | 2003-04-16 | 哈木拉提·吾甫尔 | Ripening agent and eliminating agent for abnormal black balliary matter and its preparation method |
Non-Patent Citations (3)
| Title |
|---|
| 玛依努尔·艾力 等.维药异常黑胆质成熟剂总黄酮对Bel/Fu细胞株多药耐药性及mRNA表达的影响.《中国中医药信息杂志》.2007,第14卷(第11期),27-28. * |
| 阿不都热依布·玉苏普 等.异常黑胆质成熟剂黄酮类化合物诱导HepG2细胞凋亡机制的研究.《中国中药杂志》.2007,第32卷(第11期),1068-1071. * |
| 阿不都热依木·玉苏甫 等.异常黑胆质成熟剂乙酸乙酯萃取物对HepG2细胞生长和凋亡及相关基因调控的研究.《中国药科大学 学报》.2006,第37卷(第2期),161-164. * |
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