CN101368947A - Immune globulin A detection reagent - Google Patents
Immune globulin A detection reagent Download PDFInfo
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- CN101368947A CN101368947A CNA200810121276XA CN200810121276A CN101368947A CN 101368947 A CN101368947 A CN 101368947A CN A200810121276X A CNA200810121276X A CN A200810121276XA CN 200810121276 A CN200810121276 A CN 200810121276A CN 101368947 A CN101368947 A CN 101368947A
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- iga
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- detection reagent
- immune globulin
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Abstract
The invention discloses an immunoglobulin A detection reagent, comprising an IgA reagent, an anti-IgA antibody reagent and a liquid serotype constant value calibration agent; wherein, the IgA reagent enables the IgA antigenic sites in the sample to be fully exposed so as to facilitate the full combination with the anti-IgA antibody reagent; the anti-IgA antibody reagent has high idiosyncrasy with the IgA antigens in human serum; and the liquid serotype constant value calibration agent is compared with the sample for result calculation. The anti-IgA antibodies are from sheep, horses, rats or rabbits and other mammals. The IgA reagent and the anti-IgA antibody reagent can be used as two separate reagents to compose the double-reagent form of the product, and can also be mixed based on a certain percentage to constitute the single-reagent form of the product.
Description
Technical field
The present invention relates to a kind of agent combination that is used to measure the human body fluid composition, particularly measure the detectable of the immunoglobulin A (IgA) in the human serum, can be widely used in medical science and technological field of biochemistry.
Background technology
Immunoglobulin (Ig) is one group of protein with antibody activity, mainly is present in the blood plasma, also sees in other body fluid, tissue and some juices.Immunoglobulin (Ig) great majority in the human plasma are present in the gamma globulin (gamma globulin).Can be divided into five classes, i.e. immunoglobulin G (IgG), immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin D (IgD) and immunoglobulin E (IgE).The content of IgA is only second to IgG in normal human serum, accounts for 10～20% of serum immune globulin content.From structure, IgA has the branch of monomer, binary, trisome and polymer.Be divided into two kinds of serotype and secreting types again by its immunologic function.Serotype IgA is present in the serum, and its content accounts for about 85% of total IgA.Though serotype IgA has some function of IgG and IgM, in serum, do not show important immunologic function.Secretory IgA is present in the juice, as saliva, tear, colostrum, nose and bronchus juice, gastro-intestinal Fluid, urine, sweat etc.Secretory IgA is the main antibody of the local anti-infectious immunity of body mucous membrane.So claim mucous membrane local antibody again.IgA can not pass through placenta.No IgA antibody in neonate's serum, but can from breast milk, obtain secretory IgA.After the neonate was born 4～6 months, IgA can occur in the blood, raise gradually later on, peak to adolescence.Under the normal condition, IgA antibody is not had an effect to organizing of people, and only under pathologic condition, people's normal structure changes, and has formed antigenicity, and then body has just produced corresponding antibody combination with it.A lot of diseases, especially immunity disease, the variation of immunoglobulin content usually takes place, as as seen its content reduction in serum of immunodefiiciency disease (as acquired immune deficiency syndrome (AIDS)), the immunoglobulin (Ig) of autoimmune disease (as lupus erythematosus, rheumatoid arthritis etc.) raises, in some infectious diseases, immunoglobulin content also can occur and increase.
The method of known mensuration immunoglobulin A (IgA) has SRID, immunoelectrophoresis, radio immunoassay, these methods all to exist complex operation, need special equipment, sample needs pre-service, can not carry out the batch sample analysis and can not directly go up shortcoming such as automatic clinical chemistry analyzer detection.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of IgA detectable is provided, its have sample need not to dilute, simple to operate, adopt the advantage that does not need every batch of change parameter, accuracy height, good reproducibility, antijamming capability is strong and is applicable to various types of automatic clinical chemistry analyzers behind the serotype liquid steady state value calibration agent.
In order to achieve the above object, the invention discloses a kind of immune globulin A detection reagent, mainly comprise following composition:
Thereby a, a kind ofly the IgA antigen site is fully exposed help and the well-bound IgA reactant of anti-IgA antibody reagent, comprise that accounting for its mass percent is: 0.01-1% antiseptic, 0.05-1% stabilizing agent, 0.1-10% electrolyte, 2-8% macromolecule accelerator, 0.1-10% surfactant and 0.1-0.5% reaction promoter also comprise the 5-200mmol/L buffering agent;
IgA antigen in b, a kind of and human serum has the reactive anti-IgA antibody reagent of high special, comprise that accounting for its mass percent is: the anti-IgA antibody of 5-50%, 0.001-0.5% antioxidant and 0.05-20% stabilizing agent, also comprise 0.01-1% antiseptic, 0.1-10% electrolyte, 2-8% macromolecule accelerator, 0.1-10% surfactant, also comprise the 5-200mmol/L buffering agent;
C, a kind ofly be used for, carry out the liquid serotype steady state value calibration agent that the result calculates with the sample comparison, comprise that accounting for its mass percent is: 0.01-1% antiseptic and 0.1-1% stabilizing agent, 0.02～0.6%IgA antigen and 0.05-0.1% mildewproof agent also comprise the 20-100mmol/L buffering agent.
Anti-IgA antibody among the present invention is from mammals such as sheep, horse, mouse or rabbits.
Stabilizing agent in the anti-IgA antibody reagent among the present invention can be made of in disodium ethylene diamine tetraacetate, magnesium chloride, ox or human serum albumins ethylene glycol, mannitol, the trehalose one or more.
Damping fluid among the present invention is selected from phosphate buffer, TRIS buffer, PIPES damping fluid, HEPES damping fluid, TAPS damping fluid, glycine buffer, borate buffer, and pH value is 5-10.
Damping fluid preferably phosphoric acid salt buffer among the present invention, the pH value of damping fluid is 7.4-8.1.
Surfactant among the present invention is selected from non-ionic surfactant, cationic surfactant, anionic surfactant or zwitterionic surfactant.
Surfactant preferred nonionic surfactants among the present invention.
Electrolyte among the present invention can be negative ion or kation.
Sodium chloride in the electrolyte preferred cationic among the present invention.
Antiseptic among the present invention is selected from Sodium azide, ethyl mercury sodium thiosulfate, P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate, phenol, wide-spectrum bactericide.
Principle of the present invention is to utilize antigen-antibody reaction, adds reactant, and promptly sample diluting liquid is removed electronic shell and hydrated sheath around the antigen in the sample, and antigen site is fully exposed, and adds anti-IgA antibody reagent then; Corresponding IgA antigen-reactive in high atopic anti-IgA antibody reagent and the sample, form insoluble antigen-antibody complex, produce certain turbidity, its turbidity height is directly proportional with IgA content in the sample, under provision wavelengths, measure the absorbance of this insoluble antigen-antibody complex, compare with the calibration agent of known constant, pass through formula
In the formula: Δ A
UFor being the sample hose absorbance of contrast with blank pipe absorbance
Δ A
SFor being the calibration tube absorbance of contrast with blank pipe absorbance
C
SConcentration for IgA in the calibration agent
Can calculate the content of IgA in the sample.
The inventive method and reagent are simple to operate, sample need not dilute in advance, do not need every batch of change parameter after adopting serotype liquid constant value calibration solution, and accuracy height, good reproducibility, antijamming capability are strong.And be applicable to various types of automatic clinical chemistry analyzers.
Embodiment
Immune globulin A detection reagent provided by the present invention comprises following composition:
Thereby a, a kind ofly the IgA antigen site is fully exposed help and the well-bound IgA reactant of anti-IgA antibody reagent, comprise that accounting for its mass percent is: 0.01-1% antiseptic, 0.05-1% stabilizing agent, 0.1-10% electrolyte, 2-8% macromolecule accelerator, 0.1-10% surfactant and 0.1-0.5% reaction promoter also comprise the 5-200mmol/L buffering agent.
Buffering agent can be phosphate buffer, TRIS buffer, PIPES damping fluid, HEPES damping fluid, TAPS damping fluid, glycine buffer or borate buffer etc., preferably phosphoric acid salt buffer of the present invention, the pH value of damping fluid is 5-10, the preferred pH value of the present invention is 7.4-8.1, and is best in this scope internal reaction effect;
Antiseptic can be Sodium azide, ethyl mercury sodium thiosulfate, P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate, phenol or wide-spectrum bactericide, and the present invention considers from environmental angle, preferred wide-spectrum bactericide;
Stabilizing agent can be disodium ethylene diamine tetraacetate, magnesium chloride, ox (people) seralbumin ethylene glycol, mannitol or trehalose;
Electrolytical can be negative ion or kation, the sodium chloride in the preferred cationic of the present invention (NaCl);
Accelerator can be Macrogol 2000, Macrogol 4000, Macrogol 6000 or polyglycol 8000, the preferred Macrogol 6000 of the present invention;
Surfactant can be non-ionic surfactant, cationic surfactant, anionic surfactant or zwitterionic surfactant, preferred nonionic surfactants of the present invention, comprise: Theist, Tween series, polyoxyethylene laurel ether series, polyoxyethylene phenyl ether, polyoxyethylene octyl group phenylate, polyoxyethylene alkyl phenyl ether, polyoxyethylene nonylplenyl ether etc., these surfactants can use separately, also can two or more mix use;
Reaction promoter can be hexadimethrine bromide or polybrene;
IgA antigen in b, a kind of and human serum has the reactive anti-IaA antibody reagent of high special, comprise that accounting for its mass percent is: the anti-IgA antibody of 5-50%, 0.001-0.5% antioxidant and 0.05-20% stabilizing agent, also comprise 0.01-1% antiseptic, 0.1-10% electrolyte, 2-8% macromolecule accelerator, 0.1-10% surfactant, also comprise the 5-200mmol/L buffering agent.
Anti-IgA antibody can be goat-anti people IgA antiserum, the anti-people IgA of horse antiserum, mouse-anti people IgA antiserum, the anti-people IgA of rabbit antiserum etc., and the equal buyable of these serum obtains, and also can make by oneself;
Antioxidant can be butylated hydroxy anisole, dilauryl thiodipropionate, dibutyl hydroxy toluene, benzene polyphenol, Radix Glycyrrhizae polyphenoils, phosphatide etc.Antioxidant can be dissolved in earlier in 10-100ml propylene glycol or the ethanol, joins then in the anti-IgA antibody reagent;
Buffering agent can be phosphate buffer, TRIS buffer, PIPES damping fluid, HEPES damping fluid, TAPS damping fluid, glycine buffer or borate buffer etc., preferably phosphoric acid salt buffer of the present invention, the pH value of damping fluid is 5-10, the preferred pH value of the present invention is 7.4-8.1, and is best in this scope internal reaction effect;
Antiseptic can be Sodium azide, ethyl mercury sodium thiosulfate, P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate, phenol or wide-spectrum bactericide, and the present invention considers from environmental angle, preferred wide-spectrum bactericide;
Stabilizing agent can be disodium ethylene diamine tetraacetate, magnesium chloride, ox (people) seralbumin ethylene glycol, mannitol or trehalose;
Electrolytical can be negative ion or kation, the sodium chloride in the preferred cationic of the present invention (NaCl);
Accelerator can be Macrogol 2000, Macrogol 4000, Macrogol 6000 or polyglycol 8000, the preferred Macrogol 6000 of the present invention;
Surfactant can be non-ionic surfactant, cationic surfactant, anionic surfactant or zwitterionic surfactant, preferred nonionic surfactants of the present invention, comprise: Theist, Tween series, polyoxyethylene laurel ether series, polyoxyethylene phenyl ether, polyoxyethylene octyl group phenylate, polyoxyethylene alkyl phenyl ether, polyoxyethylene nonylplenyl ether etc., these surfactants can use separately, also can two or more mix use;
C, a kind ofly be used for, carry out the liquid serotype steady state value calibration agent that the result calculates with the sample comparison, comprise that accounting for its mass percent is: 0.01-1% antiseptic and 0.1-1% stabilizing agent, 0.02～0.6%IgA antigen and 0.05-0.1% mildewproof agent also comprise the 20-100mmol/L buffering agent.
Buffering agent can be PBS, Tris, TAPS,, HEPPS, CHES, CAPS, CAPSO, POPSO, Tricie, glycocoll, glycylglycine, Diglycocol, borate etc.;
Antiseptic can be Sodium azide, ethyl mercury sodium thiosulfate, P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate, phenol, wide-spectrum bactericide etc., can use separately or several mixing use;
Mildewproof agent can be sodium propionate, deoxidation acetate etc., also can use separately or several mixing use;
Stabilizing agent can be disodium ethylene diamine tetraacetate, magnesium chloride, ox (people) seralbumin, glycerine, monose, polysaccharide etc., can use separately or several mixing use.
The equal buyable of above-described all biochemical raw materials and reagent obtains.When using as double reagent, IgA reactant and anti-IgA antibody reagent can carry out combination collocation by the volume ratio of 3:1, also can be 4:1,5:1 or 7:1.
The IgA concentration value has following selection among the present invention:
The 1st is 0g/L, replaces with physiology salt;
The 2nd is 0.20-0.8g/L, the preferred 0.6g/L of the present invention;
The 3rd 1.0-2.0g/L, the preferred 1.5g/L of the present invention;
The 4th 2.5-3.5g/L, the preferred 3.0g/L of the present invention;
The 5th 4.0-6.0g/L; The preferred 4.5g/L of the present invention.
Liquid serotype IgA steady state value calibration agent adds corresponding IgA antigen according to above-mentioned different IgA concentration value.The directly outsourcing of IgA antigen, perhaps self-control.Standard configuration of the present invention is disposed 4 bottles of calibration agents that IgA concentration is different for each above-mentioned double reagent and/or single reagent.
Below be the preparation method of IgA detectable among the present invention, product term of validity under 2～8 ℃ of lucifuge conditions is 1 year.
Embodiment one:
1, IgA reactant (R1)
Phosphate (buffering agent) 100mmol/L
Polyoxyethylene laurel ether (surfactant) 5%
NaCL (electrolyte) 10%
PEG-6000 (macromolecule accelerator) 4%
Wide-spectrum bactericide (antiseptic) 0.1%
Hexadimethrine bromide (reaction promoter) 0.3%
Disodium ethylene diamine tetraacetate (stabilizing agent) 0.05%
2, anti-IgA antibody reagent (R2)
Phosphate (buffering agent) 100mmol/L
Polyoxyethylene laurel ether (surfactant) 5%
NaCL (electrolyte) 10%
PEG-6000 (macromolecule accelerator) 4%
Wide-spectrum bactericide (antiseptic) 0.4%
Disodium ethylene diamine tetraacetate (stabilizing agent) 0.05%
The anti-people IgA of rabbit antiserum 10%
Butylated hydroxy anisole (antioxidant) 0.01%
Mannitol (stabilizing agent) 5%
3, liquid serotype IgA steady state value calibration agent
CAPSO (buffering agent) 100mmol/L
Wide-spectrum bactericide (antiseptic) 0.01%
Disodium ethylene diamine tetraacetate (stabilizing agent) 0.1%
Bovine serum albumin(BSA) (stabilizing agent) 0.5%.
Sodium propionate (mildewproof agent) 0.05%
Is 0g/L with IgA antigen according to the 1st, and the 2nd is 0.6g/L; The 3rd is 1.5g/L; The 4th is 3.0g/L; Use the filter membrane suction filtration degerming of 0.22 μ m then for 4.5g/L adds in the above-mentioned solution, put 2-8 ℃ of preservation for the 5th.Also reagent (R1) can be mixed by the volume ratio of 3:1 with (R2), to use as single agents.
Embodiment two:
1, IgA reactant (R1)
Phosphate (buffering agent) 5mmol/L
Polyoxyethylene laurel ether (surfactant) 0.1%
NaCL (electrolyte) 0.1%
PEG-6000 (macromolecule accelerator) 7%
Wide-spectrum bactericide (antiseptic) 0.01%
Polybrene (reaction promoter) 0.1%
Disodium ethylene diamine tetraacetate (stabilizing agent) 1.0%
2, anti-IgA antibody reagent (R2)
Phosphate (buffering agent) 5mmol/L
Polyoxyethylene laurel ether (surfactant) 0.1%
NaCL (electrolyte) 0.1%
PEG-6000 (macromolecule accelerator) 7%
Wide-spectrum bactericide (antiseptic) 0.01%
Disodium ethylene diamine tetraacetate (stabilizing agent) 2%
Goat-anti people IgA antiserum 50%
Butylated hydroxy anisole (antioxidant) 0.5%
Magnesium chloride (stabilizing agent) 1%
3, liquid serotype steady state value IgA calibration agent
CAPSO (buffering agent) 20mmol/L
Wide-spectrum bactericide (antiseptic) 1%
Glycerine (stabilizing agent) 1%
Bovine serum albumin(BSA) (stabilizing agent) 1%
Deoxidation acetate (mildewproof agent) 0.1%
Is 0g/L with IgA antigen according to the 1st, and the 2nd is 0.6g/L; The 3rd is 1.5g/L; The 4th is 3.0g/L; Use the filter membrane suction filtration degerming of 0.22 μ m then for 4.5g/L adds in the above-mentioned solution, put 2-8 ℃ of preservation for the 5th.Also reagent (R1) can be mixed by the volume ratio of 7:1 with (R2), to use as single agents.
Embodiment three:
1, IgA reactant (R1)
Phosphate (buffering agent) 200mmol/L
Polyoxyethylene laurel ether (surfactant) 10%
NaCL (electrolyte) 5%
PEG-6000 (macromolecule accelerator) 2%
Wide-spectrum bactericide (antiseptic) 1%
Polybrene (reaction promoter) 0.5%
Disodium ethylene diamine tetraacetate (stabilizing agent) 1%
2, anti-IgA antibody reagent (R2)
Phosphate (buffering agent) 200mmol/L
Polyoxyethylene laurel ether (surfactant) 10%
NaCL (electrolyte) 5%
PEG-6000 (macromolecule accelerator) 2%
Wide-spectrum bactericide (antiseptic) 1%
Disodium ethylene diamine tetraacetate (stabilizing agent) 0.05%
The anti-people IgA of horse antiserum 20%
Dibutyl hydroxy toluene (antioxidant) 0.3%
Glycerine (stabilizing agent) 10%
3, liquid serotype steady state value IgA calibration agent
CAPSO (buffering agent) 100mmol/L
Wide-spectrum bactericide (antiseptic) 0.06%
Disodium ethylene diamine tetraacetate (stabilizing agent) 0.5%
Bovine serum albumin(BSA) (stabilizing agent) 0.5%.
Sodium propionate (mildewproof agent) 0.8%
Is 0g/L with IgA antigen according to the 1st, and the 2nd is 0.6g/L; The 3rd is 1.5g/L; The 4th is 3.0g/L; Use the filter membrane suction filtration degerming of 0.22 μ m then for 4.5g/L adds in the above-mentioned solution, put 2-8 ℃ of preservation for the 5th.Also reagent (R1) can be mixed by the volume ratio of 4:1 with (R2), to use as single agents.
Below be the concrete operations step of IgA content in the working sample, detecting instrument is for having the 340nm wavelength, the Biochemical Analyzer of 37 ℃ of thermostats:
One, double reagent basic operation:
The result who measures is calculated by following formula:
In the formula: Δ A
UFor being the sample hose absorbance of contrast with blank pipe absorbance
Δ A
SFor being the calibration tube absorbance of contrast with blank pipe absorbance
C
SConcentration for IgA in the calibration agent
Just can calculate the IgA content in this sample.
Two, single reagent basic operation:
The result who measures is calculated by following formula:
In the formula: Δ A
UFor being the sample hose absorbance of contrast with blank pipe absorbance
Δ A
SFor being the calibration tube absorbance of contrast with blank pipe absorbance
C
SConcentration for IgA in the calibration agent
Just can calculate the IgA content in this sample.
Claims (10)
1. immune globulin A detection reagent comprises:
Thereby a, a kind ofly the IgA antigen site is fully exposed help and the well-bound IgA reactant of anti-IgA antibody reagent, comprise that accounting for its mass percent is: 0.01-1% antiseptic, 0.05-1% stabilizing agent, 0.1-10% electrolyte, 2-8% macromolecule accelerator, 0.1-10% surfactant and 0.1-0.5% reaction promoter also comprise the 5-200mmol/L buffering agent;
IgA antigen in b, a kind of and human serum has the reactive anti-IgA antibody reagent of high special, comprise that accounting for its mass percent is: the anti-IgA antibody of 5-50%, 0.001-0.5% antioxidant and 0.05-20% stabilizing agent, also comprise 0.01-1% antiseptic, 0.1-10% electrolyte, 2-8% macromolecule accelerator, 0.1-10% surfactant, also comprise the 5-200mmol/L buffering agent;
C, a kind ofly be used for, carry out the liquid serotype steady state value calibration agent that the result calculates with the sample comparison, comprise that accounting for its mass percent is: 0.01-1% antiseptic and 0.1-1% stabilizing agent, 0.02～0.6%IgA antigen and 0.05-0.1% mildewproof agent also comprise the 20-100mmol/L buffering agent.
2. immune globulin A detection reagent according to claim 1 is characterized by: described anti-IgA antibody is from mammals such as sheep, horse, mouse or rabbits.
3. immune globulin A detection reagent according to claim 1 is characterized by: the stabilizing agent in the described anti-IgA antibody reagent can be made of in disodium ethylene diamine tetraacetate, magnesium chloride, ox or human serum albumins ethylene glycol, mannitol, the trehalose one or more.
4. immune globulin A detection reagent according to claim 1, it is characterized by: described damping fluid is selected from phosphate buffer, TRIS buffer, PIPES damping fluid, HEPES damping fluid, TAPS damping fluid, glycine buffer, borate buffer, and pH value is 5-10.
5. immune globulin A detection reagent according to claim 4 is characterized by: described damping fluid is a phosphate buffer, and the pH value of damping fluid is 7.4-8.1.
6. immune globulin A detection reagent according to claim 1 is characterized by: described surfactant is selected from non-ionic surfactant, cationic surfactant, anionic surfactant or zwitterionic surfactant.
7. immune globulin A detection reagent according to claim 6 is characterized by: described surfactant is a non-ionic surfactant.
8. immune globulin A detection reagent according to claim 1 is characterized by: described electrolyte can be negative ion or kation.
9. immune globulin A detection reagent according to claim 8 is characterized by: described electrolyte is the sodium chloride in the kation.
10. immune globulin A detection reagent according to claim 1 is characterized by: described antiseptic is selected from Sodium azide, ethyl mercury sodium thiosulfate, P-hydroxybenzoic acid, ethyl-para-hydroxybenzoate, phenol, wide-spectrum bactericide.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA200810121276XA CN101368947A (en) | 2008-09-24 | 2008-09-24 | Immune globulin A detection reagent |
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| CNA200810121276XA CN101368947A (en) | 2008-09-24 | 2008-09-24 | Immune globulin A detection reagent |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102892885A (en) * | 2010-05-24 | 2013-01-23 | 国立大学法人鹿儿岛大学 | Iga-binding peptide and iga purification using same |
| CN104049085A (en) * | 2013-03-13 | 2014-09-17 | 苏州德沃生物技术有限公司 | CRP latex-reinforced immunonephelometry reagent, its kit and use of kit |
| CN105785046A (en) * | 2016-04-13 | 2016-07-20 | 柏荣诊断产品（上海）有限公司 | High-performance detection reagent kit for human blood immunoglobulin A |
-
2008
- 2008-09-24 CN CNA200810121276XA patent/CN101368947A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102892885A (en) * | 2010-05-24 | 2013-01-23 | 国立大学法人鹿儿岛大学 | Iga-binding peptide and iga purification using same |
| CN102892885B (en) * | 2010-05-24 | 2015-08-19 | 国立大学法人鹿儿岛大学 | IgA binding peptide and utilize its purifying of IgA |
| CN104049085A (en) * | 2013-03-13 | 2014-09-17 | 苏州德沃生物技术有限公司 | CRP latex-reinforced immunonephelometry reagent, its kit and use of kit |
| CN104049085B (en) * | 2013-03-13 | 2016-03-16 | 苏州德沃生物技术有限公司 | A kind of CRP latex enhancing immune turbidimetric assay reagent and kit thereof |
| CN105785046A (en) * | 2016-04-13 | 2016-07-20 | 柏荣诊断产品（上海）有限公司 | High-performance detection reagent kit for human blood immunoglobulin A |
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Application publication date: 20090218 |