CN101363869A - Method for measuring serum total protein by double-reagent biuret method - Google Patents
Method for measuring serum total protein by double-reagent biuret method Download PDFInfo
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- CN101363869A CN101363869A CNA2007100778889A CN200710077888A CN101363869A CN 101363869 A CN101363869 A CN 101363869A CN A2007100778889 A CNA2007100778889 A CN A2007100778889A CN 200710077888 A CN200710077888 A CN 200710077888A CN 101363869 A CN101363869 A CN 101363869A
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Abstract
The invention provides a measurement method of total serum protein double reagent biuret. The measurement method prepares the double reagent while modifying measurement parameter without changing single reagent reaction principle. The double reagent comprises a first solution and a second solution. The first solution is biuret blank reagent, and does not contain copper sulfate. The second solution is made by increasing copper sulfate in the prior single reagent by four times, and dissolving into the first solution. The first solution is added with an automatic biochemical analyzer, then a specimen is added, and finally the second solution is added. The measurement method can measure the optical density in different periods, completely eliminate the disturbance of lipaemia and icterus, and obviously reduce hemolytic effect.
Description
Technical field:
The present invention relates to the total serum protein assay method, belong to the laboratory medicine technical field.
Background technology:
At present, total serum protein mensuration conventional method is to adopt full automatic biochemical apparatus single reagent biuret method.This method can not be eliminated the interference of piarhemia, jaundice, haemolysis effectively, and measured value usually surpasses term of reference, though " the blank pipe of sample " eliminates interference by hand, is unsuitable for automatic analysis.
Summary of the invention:
Purpose of the present invention just provides a kind of method for measuring serum total protein by double-reagent biuret method, and the sample that this method can be eliminated piarhemia, jaundice, haemolysis can be analyzed on automatic biochemical analyzer automatically to measuring the interference of total protein, provides accurate assay for clinical.
The present invention is on the basis that does not change the single reagent reaction principle, and the preparation double reagent is revised location parameter simultaneously.The single reagent of described present usefulness is that Shanghai Kehua Bio-engineering Co., Ltd produces, and the reagent composition is with " national clinical examination working specification " second edition.
Concrete operations step of the present invention is as follows:
1, double reagent configuration: first liquid (R1) be the biuret blank reagent, sulfur acid copper not, and second liquid (R2) general is dissolved in after the copper sulphate amount is increased to four times in the single reagent of usefulness now in first liquid again and gets;
2, assay method: utilize automatic biochemical analyzer to add first liquid earlier, add sample again, add second liquid at last, three times of the consumption that the described first liquid consumption is second liquid.Described second liquid just is reduced into the single reagent of present usefulness after the 1:3 ratio is mixed by weight with first liquid;
3, parameter modification is provided with single, double reagent parameter (seeing Table) respectively on Tokyo medical treatment 1024i full automatic biochemical apparatus, calibrates respectively, measures total protein respectively, does RANDOX 1557,1558 Quality Controls simultaneously.
The table total serum protein is measured major parameter and is changed
The present invention can measure the optical density of different times, eliminates the interference of piarhemia, jaundice fully, obviously reduces the influence of haemolysis.Do not changing existing reaction principle, guarantee on the basis of existing assay method advantage, eliminate the interference that piarhemia, jaundice, haemolysis are measured total protein preferably, and the amount ratio of setting R1 and R2 is 3:1, help the proceed step by step that full automatic biochemical apparatus reagent adds, also help improving result's accuracy.
Claims (1)
1. method for measuring serum total protein by double-reagent biuret method, its method is as follows:
(1) double reagent configuration: first liquid is the biuret blank reagent, and sulfur acid copper not, second liquid are dissolved in after the copper sulphate amount is increased to four times in the single reagent of usefulness now in first liquid again and get;
(2) assay method: utilize automatic biochemical analyzer to add first liquid earlier, add sample again, add second liquid at last, three times of the consumption that the described first liquid consumption is second liquid;
(3) parameter modification: on Tokyo medical treatment 1024i full automatic biochemical apparatus, single, double reagent parameter is set respectively, calibrates respectively, measure total protein respectively, do RANDOX 1557,1558 Quality Controls simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100778889A CN101363869A (en) | 2007-08-07 | 2007-08-07 | Method for measuring serum total protein by double-reagent biuret method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100778889A CN101363869A (en) | 2007-08-07 | 2007-08-07 | Method for measuring serum total protein by double-reagent biuret method |
Publications (1)
| Publication Number | Publication Date |
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| CN101363869A true CN101363869A (en) | 2009-02-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CNA2007100778889A Pending CN101363869A (en) | 2007-08-07 | 2007-08-07 | Method for measuring serum total protein by double-reagent biuret method |
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| CN (1) | CN101363869A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102944525A (en) * | 2012-11-02 | 2013-02-27 | 上海市东方医院 | Preparation method of nickel-biuret kit, its product and application method |
| CN103278469A (en) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | Total protein detection reagent |
-
2007
- 2007-08-07 CN CNA2007100778889A patent/CN101363869A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102944525A (en) * | 2012-11-02 | 2013-02-27 | 上海市东方医院 | Preparation method of nickel-biuret kit, its product and application method |
| CN102944525B (en) * | 2012-11-02 | 2015-03-11 | 上海市东方医院 | Preparation method of nickel-biuret kit, its product and application method |
| CN103278469A (en) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | Total protein detection reagent |
| CN103278469B (en) * | 2013-05-24 | 2015-08-12 | 宁波美康生物科技股份有限公司 | A kind of total protein detection reagent |
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Open date: 20090211 |