CN101361953A - Use of Sini decoction in preparing medicine for treating hypothyroidism sexual function reduction - Google Patents
Use of Sini decoction in preparing medicine for treating hypothyroidism sexual function reduction Download PDFInfo
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- CN101361953A CN101361953A CNA2008100794528A CN200810079452A CN101361953A CN 101361953 A CN101361953 A CN 101361953A CN A2008100794528 A CNA2008100794528 A CN A2008100794528A CN 200810079452 A CN200810079452 A CN 200810079452A CN 101361953 A CN101361953 A CN 101361953A
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Abstract
The invention relates to an application of a traditional Chinese drug composite, namely a sini decoction, in preparing a drug for treating hypothyroid sexual function reduction. When researching the effect of the sini decoction on rat models with hypothyroid sexual function reduction, the rats are found to experience obvious increase of testosterone level, obvious rise of T level in blood serum after being lavaged with the sini decoction; meanwhile, the level of thyroid hormone gradually recovers, thus indicating that the sini decoction can improve the generating function of rats with hypothyroid sexual function reduction and enhance sexual function by adjusting the secretion of sex hormone. The invention adds the new application of the sini decoction in treating hypothyrosis, especially hypothyroid sexual function reduction and extends indications for the traditional Chinese drug composite, namely the sini decoction.
Description
Technical field
The present invention relates to a kind of new purposes of Chinese medicine preparation decoction for Resuscitation, especially relate to the application of decoction for Resuscitation in the medicine that preparation treatment hypothyroidism sexual function reduces.
Background technology
Decoction for Resuscitation comes from treatise on Febrile Diseases, is made up of Radix Aconiti Lateralis Preparata, Rhizoma Zingiberis, Radix Glycyrrhizae.Treatise on Febrile Diseases is lunar for sun deteriorated case change to belong to, or thirty years of age this side of cold transformation card such as lunar, few the moon, gets its recuperating depleted YANG and rescuing the patient from collapse, strengthening body resistance to win the effect of YIN-cold, is used for the treatment of yin-cold excess, yang-energy is on the decline, faints contraryly and extremity occur, and aversion to cold is tired to crouch, contracture in the abdomen, lethargy, watery diarrhea with indigested food, vomiting can not be taken food, sweating, diseases such as faint and thready pulse.Now be usually used in treating due to acute cardiogenic shock and a variety of causes circulatory failure person that belongs to the assertive evidence.
Decoction for Resuscitation enjoys ancient Chinese medicine doctor to praise highly, and about its effect and application, at different times report is arranged all.The 60 to 70's of last century only is confined to its clinical practice; The eighties begins to probe into by pharmacological evaluation, but also only is to carry out preliminary observation from basic physiological property; The nineties, the domestic scholar of having uses modern scientific method and advanced equipment, illustrates its composition principle and scientific meaning thereof from the material base angle, discloses the effect of each flavour of a drug in the side; This century, existing scholar attempted using modern molecular biology technique and achievement to explain the mechanism of its effect.
1, pharmacological action
Decoction for Resuscitation mainly has heart tonifying, boosts, shock, resist myocardial ischemia and effect such as lipid peroxidation, raise immunity, anoxia enduring, analgesia, antiinflammatory.
1.1 cardiovascular system
Decoction for Resuscitation is to aspects such as the effect of cardiovascular system is mainly reflected in antioxidation, heart tonifying and shock, resists myocardial ischemia, anti-hypoxia, atherosclerosis, anti-stress cause cardiac damage and lipid peroxidations.
1.1.1 antioxidation
Wu Weikang (Pharmacology and Clinics of Chinese Materia Medica .1994, (5): 1) can significantly increase stress Aged Mice SOD of heart tissue activity for the report decoction for Resuscitation, prevents that oxygen-derived free radicals from producing, and improves the myocardial blood perfusion, thereby to stress Aged Mice heart performance protective effect.
1.1.2 heart tonifying and shock
Decoction for Resuscitation all has effect, the effectively development of countershock to each phase of shock.Experiment finds, occurs in early days at rat shock that extremity are livid purple, body temperature is low, gives decoction for Resuscitation when oliguria or anuria, and then the extremity color changes red, urine amount showed increased.(Chinese Pharmaceutical Journal .2003 such as Shao Chunhong; 38 (11): 847-850) the report decoction for Resuscitation has tangible heart tonifying, boosting to heart source property and hemorrhagic shock; can obviously strengthen the contractility of rabbit, and can increase myocardial blood flow by coronary artery dilating, improve approach such as myocardial oxygen delivery and energy metabolism and free radical resisting protection cardiac muscle at body-centered flesh and isolated myocardium.Identical text report decoction for Resuscitation can significantly reduce ascorbic content among the adrenal gland, increases blood plasma cortisol content, produces diuresis rapidly.(Chinese Chinese medicine information magazine .2004,11 (10): 922-923) (Chinese medicine research .1999,15 (4): 60-62) the report decoction for Resuscitation blood pressure of endotoxin shock rabbit that can raise, to contain demethylcoclaurine relevant with it in this effect with Xiao Fengxia for Chen Xinyi etc.
1.1.3 resist myocardial ischemia and oxygen lack resistant function
(Chinese herbal medicine .1995,26 (3): 141) report that the myocardial ischemia animal model that decoction for Resuscitation causes pituitrin has significant improvement effect, the ST section moves down significantly and alleviates Wu Weikang, and the rising of T ripple obviously is subjected to press down.Wu Weikang (Chinese Journal of Pathophysiology .1998,14 (6): 634) when inquiring into its effect that improves the ischemic myocardium energy metabolism and mechanism, discovery is compared with ischemia group, the ischemic myocardium mitochondrial injury of decoction for Resuscitation+ischemia group significantly alleviates, glycogen use significantly reduces, lactic acid concn significantly descends, and the myocardial nutritional flow amount significantly rises, and concentration of oxygen free radicals significantly reduces.The proof decoction for Resuscitation has the remarkable effect that improves ischemic myocardium energy metabolism disorder, and this effect increases blood supply of cardiac muscle and eliminate oxygen-derived free radicals relevant with decoction for Resuscitation.(Chinese practical Chinese and western medicine magazine .2003 such as Qin Jian, 3 (16): 2019) the report decoction for Resuscitation can improve significantly that left ventricular diastolic function due to the acute myocardial ischemia reduces and the energy metabolism of ischemic myocardium, Liao Hui (time precious traditional Chinese medical science traditional Chinese medicines .2001,12 (3): 271-272) with Hu Rui (Tianjin Chinese medicine .2004,21 (1): 28-30) the report decoction for Resuscitation can increase cardiac output (SV) and ejection fraction (EF), improve performance of electrocardiogram myocardial ischemia and hemorheology index etc., (Chinese Journal of Pathophysiology .1999 such as arhat river, 15 (11): 994-996) the report decoction for Resuscitation reduces total plasma cholesterol (TC), serum high-density LP cholesterol (HDL-C), the effect of low-density lipoprotein cholesterol (LDL-C) obviously is better than nifedipine, and can delay the generation of myocardium cell necrosis.(CHINA JOURNAL OF CHINESE MATERIA MEDICA .2006 such as Liu Ying, 31 (15): after 1269) report is used decoction for Resuscitation pretreatment 24h, can reduce myocardial ischemia-reperfusion (MI/R) back apoptosis rate, reduce cytochrome C from mitochondrial release, raise the proteic expression of bcl-xl, suppress the activation of caspase-3, its mechanism may be relevant with the apoptotic mitochondrion signal transduction pathway of inhibition.(Chinese experimental pharmacology of Chinese medical formulae magazine .2005 such as Ma Tianshu; 11 (4): 48) neonatal rat myocardial cell of report cultivation carries out the anoxia _ reoxygenation damage again through the pretreatment of decoction for Resuscitation extract; measure lactic acid dehydrogenase (LDH) activity in intracellular plastochondria dehydrogenase activity, malonaldehyde (MDA) content and the culture fluid supernatant; decoction for Resuscitation can improve intracellular plastochondria dehydrogenase activity as a result; reduce lactic acid dehydrogenase activity and the interior MDA content of cell in the supernatant, the prompting decoction for Resuscitation all has protective effect to the myocardial cell of anoxia _ reoxygenation damage.(Chinese patent medicine .2005,27 (6): 697) report is for H for Nie Yongmei etc.
2O
2The myocardial cell injury that causes, decoction for Resuscitation can reduce the apoptosis rate and the necrosis rate of its cell, reduce myocardial cell mitochondrial membrane potential decrease speed, and its mechanism is relevant with stablizing of protective wire mitochondrial membrane potential.(Chinese crude drug .2005,28 (5): 395) report H in addition such as Nie Yongmei
2O
2Induce damage preparation neonatal rat myocardial cell oxidative stress damage model; decoction for Resuscitation can reduce the content of MDA in the cell; increase the SOD activity; reduce the apoptosis rate and the necrosis rate of cell; the prompting decoction for Resuscitation has the effect of anti-oxidation stress damage preferably; this protective effect to myocardial cell may be by increasing the expression of Bcl-xL mRNA, and the expression that reduces Bcl-xSmRNA realizes.Decoction for Resuscitation all has protective effect to ischemic myocardium from aspects such as metabolism, function, forms.
1.1.4 atherosclerosis (AS)
(Chinese arteriosclerosis magazine .2003,11 (6): 505-508) the report decoction for Resuscitation has the effect that AS forms and makes progress that suppresses to Wu Weikang etc.Give decoction for Resuscitation to AS model rabbit, the result, the aortic tunica intima plaque thickness of model rabbit, aortic tunica intima/area percentage and aortic tunica intima plaque area/middle film plaque area ratio is starkly lower than matched group, and this effect is by blood lipid regulation, removing oxygen-derived free radicals, adjusting nitric oxide/realizations such as Endothelin balance.
1.2 effect to cerebral ischemia
(Chinese Journal of Pathophysiology .2005,21 (4): 669-673) report cerebral ischemic model rat shows as function of nervous system's scoring reduction to Yan Jianyun etc., and brain water content increases, and decoction for Resuscitation can reduce the brain water content of cerebral ischemic model rat, improves its function of nervous system.Yan Jianyun etc. (Chinese molecular cardiology magazine .2004,4 (3): 168-172) the report light microscopic is observed down, and the decoction for Resuscitation group is compared with matched group, and ischemia injury obviously alleviates, and cellular morphology is near normal, and the nerve fiber traveling is clear.
1.3 raise immunity
(Chinese experimental pharmacology of Chinese medical formulae magazine .2006,12 (2): 28) the report Radix Aconiti Lateralis Preparata can increase the mice serum antibody titer and promote spleen antibody to form Ge Yingchun etc., and guinea pig serum complement content is obviously increased.The inductive inflammatory reaction of escherichia coli endotoxin (LPS), after the effect of decoction for Resuscitation extract, the Turnover of Mouse Peritoneal Macrophages phagocytic activity that the dimethyl diaminophenazine chloride method detects, decoction for Resuscitation can significantly suppress the propagation of thymic lymphocytes as a result, improve peritoneal macrophage phagocytic function and metabolic activity, reduce the growing amount of tumor necrosis factor (TNF), il-1 (IL-1), its pharmacologically active is more comprehensive.
1.4 other
(Chinese Chinese medicine information magazine .2004,11 (10): 922-923) the report decoction for Resuscitation also has effects such as antalgic and sedative to Chen Xinyi etc.
2, clinical practice
The clinical practice of decoction for Resuscitation mainly contains two aspects, the one, its tradition is used: Zhong Jing is thirty years of age this side of SHAO YIN syndrome, disease is seen extreme cold of the limbs, aversion to cold vomiting and diarrhea, god decline somnolence, pale tongue with white fur, faint pulse being about to disappear, be equivalent to a variety of causes causes in the modern medicine shock, measles serious symptom, myocardial infarction, heart failure, toxemia, acute disease profuse perspiration and see that card such as collapse belongs to the decline multiple disease of excess of YIN of sun, followingly enumerate 2.1～2.4.The one, it is newly used in modern disease: though sick non-YANG-depletion severe crisis wild such as headache, the syndrome of dyspnea, esohagismus, recurrent aphtha, acute or chronic gastritis, gastroptosis, diarrhoea, edema, heating, Raynaud disease, radioactive leukocytopenias, but card belongs to deficiency and coldness person, also obtain obvious curative effects with decoction for Resuscitation, followingly enumerate 2.5 and 2.6.
2.1 angina pectoris, myocardial infarction
Yao Xiuqin (the Shandong science and technology .1995:408 of publishing house) report decoction for Resuscitation has better curative effect to coronary heart disease, angina pectoris, can improve in coronary heart disease, the angina pectoris clinical symptoms such as chest pain is uncomfortable in chest, reduce the angina pectoris attacks frequency, reduce the nitroglycerin consumption, behind we, increased blood pressure also maintains normal level, does not fall again after can not rising, and this is we's characteristics.
2.2 bradyarrhythmia
(Hebei combination of Chinese and Western medicine magazine .1999 such as Bu Xiaoyan, 8 (3): 422) among the report cold limbs Tonga SHENGMAI SAN treatment bradyarrhythmia 17 routine patients, effective 15 examples, 2 routine sick sinus syndrome patient subjective symptomss and electrocardiogram all must not improve, and need add and use the antiarrhythmic Western medicine.
2.3 vertebral-basilar artery insufficiency
Make (Pharmacology and Clinics of Chinese Materia Medica .2004,20 (5): 44) report, among the 60 routine patients such as inferior qin, 54 examples (90%) symptom is alleviated fully, and 2 examples (3.3%) are obviously alleviated, and 3 examples (5%) are slight to be alleviated, 1 example (1.7%) is invalid, and blood flow rheology and blood lipids index have clear improvement before and after the treatment.
2.4 hypothyroidism serious symptom
Cheng Hanqiao etc. (do not control for Gansu traditional Chinese medical science .1999,12:39-40) report by the long-term delay of adult's hypothyroidism, be common in the patient of myxedema coma, show as the coma cold of the limbs, skin temperature descends, breathe humble, the muscle tension and relaxation is unable, pale tongue, corpulent tongue, faint pulse being about to disappear, control and should rouse oneself yang-energy, rescue and contraryly take off admittedly, the side adds and subtracts with Sini Jia Renshen Tang.Though this disease is called " hypothyroidism serious symptom ", the complication of not controlling for the hypothyroidism delay is different with typical adult's hypothyroidism in fact.
2.5 headache
Wang Shangjun (Hainan medical science .2006,17 (4): 113) report, the cold Sheng type headache of cold limbs Tonga flavor treatment yang deficiency, add flavor with disease: the severe headache person adds Radix Angelicae Dahuricae 10g, and insufficiency of the spleen indigestion and loss of appetite person adds Radix Codonopsis 15g, Pericarpium Citri Reticulatae 5g, greasy person adds Herba Pogostemonis 10g, Massa Medicata Fermentata 10g, Pericarpium Citri Reticulatae 5g with pale tongue with white fur.Take medicine three doses, headache transference cure person's 25 examples (74%) are taken medicine six doses, headache transference cure person's 6 examples (18%), and invalid 3 examples (8%) are obeyed six doses of nonresponders and are withdrawn.
2.6 other
Song Shucai (new traditional Chinese medical science .2004,69), Li Taisheng (the journal .1998 of Guiyang College of Traditional Chinese Medicine 36 (8):, 59), (Chinese medicine research .2001 such as Li Yongjian 20 (1):, 17 (2): 22) report, decoction for Resuscitation also can be used for treating dialectical genus deficiency and coldness persons such as intractable hyperhidrosis, night antiperspirant, arthromyodynia, epilepsy, flooding.
At present, mainly concentrate on the cardiovascular aspect, and obtained big effect about the pharmaceutical research of decoction for Resuscitation.The modern clinical practice of decoction for Resuscitation is very extensive, remove collapse such as shock, measles serious symptom, the liver abscess, card belongs to sun and declines outside the excess of YIN person, in addition the impatient chronic diseases of genus deficiency and coldness such as diarrhoea, gastroptosis, radioactive leukocytopenia, recurrent aphtha, cough with asthma is all had good curative effect.
Thyroid disease is a kind of common clinical, frequently-occurring disease, occupies second in endocrine and metabolic disease, is only second to carbohydrate metabolism disturbance.
Hypothyroidism (be called for short " hypothyroidism ") is because thyroxin is synthetic, secretion or the not enough institute of biological effect cause, few, slow in one's movements with fear of cold, antiperspirant clinically, lethargy, hypomnesis, muscle weakness, drowsiness, sexual hypofunction etc. serve as mainly to show.The method of western medical treatment hypothyroidism mainly is to adopt thyroxin replacement therapy, domestic main use thyroid tablet at present.
" hypothyroidism " when the basal metabolism level to whole body exerts an influence, also can cause the infringement of patient's libido and sexual function as a kind of endocrinopathy of general.According to relevant, male's hypothyroidism patient 80% hyposexuality, 40～50% have the erection disturbance of varying degree.Can be observed women's hypothyroidism patient clinically the ovarian activity change of various degree can take place, show as dissimilar irregular menses, menorrhagia often in early days, late period, then menstrual blood volume was few, amenorrhea is infertile then, even if the complication of becoming pregnant and also easily miscarrying or multiple mother of being unfavorable for and fetus occurring.Among the hypothyroidism patient, the male was easily sent out in 31～40 years old, and the women is easily sent out in 21～40 years old, is in the childbearing age.Infertility is absorbed in the misery many families, and the patient if can in time diagnose, and carries out rational therapy, can live well.
Yet, the Therapeutic Method of the additional thyroxin that present modern medicine is general, unsatisfactory for the curative effect that the treatment hypothyroidism sexual function reduces.The inventor is at the pharmaceutical research of decoction for Resuscitation to hypothyroidism sexual function reduction effect, find that this medicine can improve sex hormone level, reduction has therapeutical effect to hypothyroidism sexual function, is used for the treatment of the report that hypothyroidism sexual function reduces and yet there are no relevant decoction for Resuscitation in the prior art.
Summary of the invention
A kind of new purposes that the purpose of this invention is to provide the Chinese medicine decoction for Resuscitation.
What in fact, the present invention relates to is the application of decoction for Resuscitation in the medicine that preparation treatment hypothyroidism sexual function reduces.
Decoction for Resuscitation of the present invention is according to version Pharmacopoeia of the People's Republic of China record in 2005, is made by following raw material medicaments:
Radix Aconiti Lateralis Preparata 300g Rhizoma Zingiberis 200g Radix Glycyrrhizae Preparata 300g.
The medicine of above-mentioned each component can be made said dosage form on any pharmaceutics, as water decoction, pill, micropill, powder, tablet, capsule, injection etc.
The concrete preparation method of medicine of the present invention is:
Accurately take by weighing each medicine according to above dose ratio, Radix Aconiti Lateralis Preparata and Radix Glycyrrhizae Preparata are decocted with water 2 times, the 1st time 2 hours, the 2nd time 1.5 hours, collecting decoction filtered; Extract volatile oil in the Rhizoma Zingiberis with vapor distillation, device is preserved in addition; Rhizoma Zingiberis Recens dregs decocts with water 1 hour, solution behind decocting liquid and the separated volatile oil merges, filter, decocting liquid with the Radix Aconiti Lateralis Preparata Radix Glycyrrhizae merges again, be concentrated into the thick paste of relative density 1.20～1.25 (50～60 ℃), according to the conventional formulation method of the above various dosage form of pharmaceutics, add proper supplementary material and add Rhizoma Zingiberis volatile oil, make corresponding preparation.
The present invention adopts the decoction for Resuscitation water decoction to carry out pharmacology test research, can illustrate that by result of the test decoction for Resuscitation reduces the new purposes in field at the treatment hypothyroidism sexual function.Equally, using other dosage forms of being made by decoction for Resuscitation or using with decoction for Resuscitation is that the preparation of main effective ingredient all can reach same pharmacological effect, and its difference only is the difference on the use amount.
In the do time spent of research decoction for Resuscitation to the low rat model of hypothyroidism sexual function, after finding to give the rat oral gavage decoction for Resuscitation, the testosterone levels of male rat obviously improves, and thyroid hormones level is also recovered gradually simultaneously.Concrete pharmacological experiment data is as follows:
1, experiment material
Cleaning level Wistar rat, male, body weight 170 ± 10g, normal diet.In 20 ± 2 ℃, adaptation is divided into 4 groups by body weight with 40 rats: normal group, model group, decoction for Resuscitation group, thyroid tablet group, 10 every group after 6 days at random in the environment of relative humidity 40%.
Thyroid tablet, the 40mg/ sheet grinds, and is made into 0.08% suspension with distilled water.
Propylthiouracil (PTU), every contains Propylthiouracil 50mg, grinds, and is made into 0.1% solution with distilled water.
Trilute (T
3) put and exempt from medicine box, thyroxine (T
4) put exempt from medicine box, thyrotropin (TSH) is put and is exempted from medicine box, free triiodothyronine (FT
3) put and exempt from medicine box, free thyroxine (FT
4) put exempt from medicine box, thyrotropin (sTSH) put exempt from medicine box, testosterone (T) is put and is exempted from medicine box.
2, experimental technique
2.1 modeling and administration
0.1% propylthiouracil (PTU) solution is given model group, decoction for Resuscitation group, thyroid tablet group rat oral gavage by 1mL/100g body weight (dosage is 10mg/kg), normal group gives the equivalent normal saline, every day 1 time, 2 weeks of continuous irrigation stomach, get blood 2mL by the total vein of rat neck, centrifugalize serum detects T
3, T
4, sTSH, determine whether success of modeling according to measurement result.
After the modeling success, 4 weeks of successive administration, to weigh weekly to adjust dosage, it is specific as follows that each organizes dosage:
Normal group: give normal saline by 1mL/100g dosage, every day 1 time;
Model group: continue to give 0.1%PTU solution, dosage is 10mg/kg, the next day 1 time (behind the PTU that stops using, it is normal that the rat thyroid function can be recovered, for keeping modeling rat thyroid hypofunction state, still become mould P of Rats TU during the treatment, method and dosage are constant, but administration change into the next day);
The decoction for Resuscitation group: give the decoction for Resuscitation water decoction, dosage is 10g (crude drug)/kg, every day 1 time, gives modeling medicine PTU same model group simultaneously;
The thyroid tablet group: give 0.08% thyroid tablet suspension, dosage is 8mg/kg, every day 1 time, gives modeling medicine PTU same model group simultaneously.
2.2 detect
2.2.1 get blood and internal organs
Treatment be can't help water 12h with the rat fasting after finishing, weighs, and anesthesia, centrifugal by abdominal aortic blood in 3000rpm, get serum and be used to detect FT
3, FT
4, sTSH, T.The testis taking-up is accurately weighed, and, obtain the testicular weight index divided by rat body weight; Testis is fixed (being used as pathologic finding) with 10% formalin solution.
2.2.2 use serum measured by radioimmunoassay FT
3, FT
4,, measure T with liquid equilibrium competition radio immunoassay with solid phase dibit point sandwich method for determining sTSH.
2.2.3 with the fixing testis of 10% formalin solution, conventional dehydration back is with paraffin embedding, shave, and HE dyeing, optical microscope is observed down, carries out pathological examination.
3, experimental result
3.1 the rat thyroid functional status after the modeling
After two weeks of modeling, the serum T of rat of model group and each administration group (being collectively referred to as the modeling group) and normal rats
3, T
4Compare remarkable decline (P＜0.01), and sTSH raises obviously (P＜0.01), show that the state of hypothyroidism, prompting modeling success (seeing Table 1) have all appearred in the rat of modeling group after the modeling.
Table 1 normal group and the comparison of modeling group rat thyroid functional parameter (x ± S)
Annotate: * and normal group be P＜0.01 relatively.
The rat thyroid functional status after 3.2 treatment finishes
Thyroid tablet group FT
3Compare no significant difference (P〉0.05) with model group, significant difference (P＜0.05), decoction for Resuscitation group FT are arranged with normal group
3Between normal group and model group, with the two difference (P＜0.05) is arranged, prompting modeling rat FT
3All do not recover normal.Decoction for Resuscitation group, thyroid tablet group FT
4And significant difference (P＜0.05) is all arranged between normal group, model group, and do not have significant difference (P〉0.05) between decoction for Resuscitation group and thyroid tablet group, illustrate that these two groups have therapeutical effect, but recover normal fully.Decoction for Resuscitation group, thyroid tablet group sTSH compare no significant difference (P〉0.05) with normal group, compared significant difference (P＜0.05) with model group, and sTSH does not have significant difference (P〉0.05) between decoction for Resuscitation group and the thyroid tablet group, point out these two groups all can make sTSH recover normal value, and therapeutical effect is near (seeing Table 2).
After finishing, table 2 treatment respectively organizes rat thyroid function (x ± s)
Annotate:
Compare P＜0.05 with normal group, * and model group be P＜0.05 relatively, and and thyroid tablet group be P＜0.05 relatively.
The rat reproductive system functional status after 3.3 treatment finishes
Notable difference (P＜0.05) is arranged between model group testosterone concentration and the normal group, no significant difference between decoction for Resuscitation group, thyroid tablet group testosterone concentration and the normal group (P〉0.05), and notable difference (P＜0.05) is arranged between the model group, prompting hypothyroidism can cause that rat gonadal hormone testosterone reduces, and decoction for Resuscitation group, thyroid tablet group all have obvious therapeutic action.The rat testicle ponderal index of model group is apparently higher than normal group (P＜0.05), each treatment group testicular weight index is between normal group and model group, with the two equal no significant difference (P〉0.05), prompting hypothyroidism can also cause the increase of rat testicle ponderal index, and each treatment group effect not obvious (seeing Table 3).
Table 3 rat gonadal hormone and the comparison of reproductive system organ index (x ± s)
Annotate:
Compare P＜0.05 with normal group, * and model group be P＜0.05 relatively.
3.4 the pathological examination results of testis
Visible testis tissue convoluted seminiferous tubule densification under the normal arrangement of mirrors, basement membrane is complete, and sustenticular cell and spermatogenic cell at different levels are normal, and intensive sperm is arranged in the convoluted seminiferous tubule, and the tube wall border is neat, and a matter does not have hyperemia, edema, and Interstitial cell is grown also normal (Fig. 1).Model group testis outward appearance increases, breaking, oozing out appears in the visible part convoluted seminiferous tubule under the mirror, the basement membrane dissolving, connective tissue between the contorted seminiferous tubules increases, Interstitial cell quantity also obviously reduces, between matter edema occurs, ooze out, sustenticular cell and each spermatogenic cell also have minimizing, and arrange loose (Fig. 2).Visible testis tissue convoluted seminiferous tubule densification under the decoction for Resuscitation arrangement of mirrors, basement membrane is complete, and sustenticular cell and spermatogenic cell at different levels are normal, and intensive sperm is arranged in the convoluted seminiferous tubule, and a matter increases (Fig. 3).Visible convoluted seminiferous tubule densification under the thyroid tablet arrangement of mirrors, a matter edema occurs, oozes out, and sustenticular cell and each spermatogenic cell also have minimizing (Fig. 4).
4, decoction for Resuscitation is summed up the therapeutical effect that hypothyroidism sexual function reduces
Experimental result shows that the testicular weight index that hypothyroidism sexual function reduces the normal rat of male rat increases, and the serum T level obviously reduces, and has the reproductive system dysfunction during prompting hypothyroidism state.After the treatment, decoction for Resuscitation group rat blood serum T level obviously raises, and shows that decoction for Resuscitation can pass through the modulability hormone secretion, improves the reproduction function that hypothyroidism sexual function reduces rat, sexual function improving.
Description of drawings
Fig. 1 is normal group testis tissue section HE dyeing (400 *);
Fig. 2 is model group testis tissue section HE dyeing (400 *);
Fig. 3 is decoction for Resuscitation group testis tissue section HE dyeing (400 *);
Fig. 4 is thyroid tablet group testis tissue section HE dyeing (400 *).
The specific embodiment
Embodiment 1
Get Radix Aconiti Lateralis Preparata 3Kg, Radix Glycyrrhizae Preparata 3Kg and mix, decoct with water 2 times, the 1st time 2 hours, the 2nd time 1.5 hours, collecting decoction filtered.
Get in the Rhizoma Zingiberis 2Kg adding vapor distillation device and carry out vapor distillation, extract volatile oil device preservation in addition.Rhizoma Zingiberis Recens dregs decocts with water 1 hour again, and the aqueous solution behind decocting liquid and the separated volatile oil merges, and filters.
Rhizoma Zingiberis decocting liquid and Radix Aconiti Lateralis Preparata Radix Glycyrrhizae decocting liquid are merged, be concentrated into the thick paste of relative density 1.20～1.25 (50～60 ℃).
Add an amount of supplementary product starch and magnesium stearate in thick paste, mix homogeneously is also made granule, after the drying to be cooled, sprays into Rhizoma Zingiberis volatile oil, is pressed into sheet under the airtight condition, and coating is made the cold limbs sheet.
Embodiment 2
Get Radix Aconiti Lateralis Preparata 3Kg, Radix Glycyrrhizae Preparata 3Kg and mix, decoct with water 2 times, the 1st time 2 hours, the 2nd time 1.5 hours, collecting decoction filtered.
Get in the Rhizoma Zingiberis 2Kg adding vapor distillation device and carry out vapor distillation, extract volatile oil device preservation in addition.Rhizoma Zingiberis Recens dregs decocts with water 1 hour again, and the aqueous solution behind decocting liquid and the separated volatile oil merges, and filters.
Rhizoma Zingiberis decocting liquid and Radix Aconiti Lateralis Preparata Radix Glycyrrhizae decocting liquid are merged, be concentrated into the thick paste of relative density 1.20～1.25 (50～60 ℃).
Add an amount of supplementary product starch in thick paste, mix homogeneously is also made granule, after the drying to be cooled, sprays into Rhizoma Zingiberis volatile oil, and is evenly mixed, makes the cold limbs capsule in incapsulating.
Embodiment 3
Get Radix Aconiti Lateralis Preparata 3Kg, Radix Glycyrrhizae Preparata 3Kg and mix, decoct with water 2 times, the 1st time 2 hours, the 2nd time 1.5 hours, collecting decoction filtered.
Get in the Rhizoma Zingiberis 2Kg adding vapor distillation device and carry out vapor distillation, extract volatile oil device preservation in addition.Rhizoma Zingiberis Recens dregs decocts with water 1 hour again, and the aqueous solution behind decocting liquid and the separated volatile oil merges, and filters.
Rhizoma Zingiberis decocting liquid and Radix Aconiti Lateralis Preparata Radix Glycyrrhizae decocting liquid are merged, be concentrated into the thick paste of relative density 1.20～1.25 (50～60 ℃).
In thick paste, add ethanol, stir, left standstill 24 hours, filter, concentrating under reduced pressure is made thick paste behind the recovery ethanol, adds the suitable quantity of water dilution, cold preservation 24 hours, filter, be added into an amount of simple syrup and antiseptic, and add above-mentioned Rhizoma Zingiberis volatile oil, thin up becomes to contain the liquid of crude drug amount 1g/mL, makes the decoction for Resuscitation water decoction.
Embodiment 4
Get Radix Aconiti Lateralis Preparata 3Kg, Radix Glycyrrhizae Preparata 3Kg and mix, decoct with water 2 times, the 1st time 2 hours, the 2nd time 1.5 hours, collecting decoction filtered.
Get in the Rhizoma Zingiberis 2Kg adding vapor distillation device and carry out vapor distillation, extract volatile oil, become firming body with beta-cyclodextrin inclusion compound.
Rhizoma Zingiberis Recens dregs decocts with water 1 hour again, and the aqueous solution behind decocting liquid and the separated volatile oil merges, and filters.
Rhizoma Zingiberis decocting liquid and Radix Aconiti Lateralis Preparata Radix Glycyrrhizae decocting liquid are merged, be concentrated into the thick paste of relative density 1.20～1.25 (50～60 ℃).
Add a certain amount of alcoholic solution in thick paste, make to contain the alcohol amount and reach 50%, stir, left standstill 24 hours, filter, concentrating under reduced pressure is made dry extract after reclaiming ethanol.
With auxiliary materials and mixing such as the beta-schardinger dextrin-firming body of dry extract, Rhizoma Zingiberis volatile oil and appropriate amount of starch, microcrystalline Cellulose, levigate and by 100 mesh sieves, be wetting agent system micropill with certain density ethanol, drying is made the cold limbs micropill behind the coating.
Claims (2)
1, the application of Chinese medicine composition decoction for Resuscitation in preparation treatment hypothyroidism sexual function reduction medicine, described Chinese medicine composition is made by the crude drug of following parts by weight:
Radix Aconiti Lateralis Preparata 300 Rhizoma Zingiberiss 200 Radix Glycyrrhizae Preparatas 300.
2, the application of Chinese medicine composition decoction for Resuscitation according to claim 1 in preparation treatment hypothyroidism sexual function reduction medicine, the dosage form of Chinese medicine composition is water decoction, pill, micropill, powder, tablet, capsule, injection.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940697A (en) * | 2012-12-13 | 2013-02-27 | 济南康众医药科技开发有限公司 | Medicine for treating allergic rhinitis and bronchial asthma |
| WO2017181987A1 (en) * | 2016-04-21 | 2017-10-26 | 张东 | Traditional chinese medicine composition effective against various symptoms, and derivative and preparation of same |
| CN110794074A (en) * | 2019-11-18 | 2020-02-14 | 广西医科大学 | Angelica sinensis Sini decoction cold-resistant blood coagulation stasis syndrome differential metabolite metabolic pathway and research method |
| CN110890160A (en) * | 2019-11-29 | 2020-03-17 | 山西中医药大学 | Arteriosclerosis obliterans prevention method based on heart governing blood vessels theory |
-
2008
- 2008-09-22 CN CNA2008100794528A patent/CN101361953A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940697A (en) * | 2012-12-13 | 2013-02-27 | 济南康众医药科技开发有限公司 | Medicine for treating allergic rhinitis and bronchial asthma |
| WO2017181987A1 (en) * | 2016-04-21 | 2017-10-26 | 张东 | Traditional chinese medicine composition effective against various symptoms, and derivative and preparation of same |
| CN107303383A (en) * | 2016-04-21 | 2017-10-31 | 张东 | A kind of Chinese medicine composition and its derivative and product to a variety of symptoms with effect |
| CN110794074A (en) * | 2019-11-18 | 2020-02-14 | 广西医科大学 | Angelica sinensis Sini decoction cold-resistant blood coagulation stasis syndrome differential metabolite metabolic pathway and research method |
| CN110890160A (en) * | 2019-11-29 | 2020-03-17 | 山西中医药大学 | Arteriosclerosis obliterans prevention method based on heart governing blood vessels theory |
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Open date: 20090211 |