CN101348489A - Valerian extract iridoidate compound - Google Patents
Valerian extract iridoidate compound Download PDFInfo
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- CN101348489A CN101348489A CNA2007100353588A CN200710035358A CN101348489A CN 101348489 A CN101348489 A CN 101348489A CN A2007100353588 A CNA2007100353588 A CN A2007100353588A CN 200710035358 A CN200710035358 A CN 200710035358A CN 101348489 A CN101348489 A CN 101348489A
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- valerian
- iridoidate
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Abstract
The invention discloses an iridoid ester compound of a valerian extract. The valerian is subjected to coarse crushing, and immersion and filtration by an ethanol solution, and the filtrate is decompressed and condensed to be a dilute extract which is subjected to vacuum drying to obtain a coarse product of valerian; the ethanol solution is determined to be an extracting agent of the valerian extract, and the extraction rate of the iridoid ester compound is 85.48 percent; the coarse product of valerian is purified and added with proper amount of water for suspension, and the suspension passes through macropore adsorbent resins and is subjected to elution by a small amount of water and elution by ethanol, the eluent collected is subjected to decompression, condensation and drying to obtain thevalerian extract. The extract has the advantages of simple component, high purity of medical components and stable capability, and the total iridoid content of the product is 86 percent.
Description
Technical field
The present invention relates to a kind of valerian extract iridoidate compound, specifically is to be that raw material carries out the separation and Extraction iridoidate compound with the valerian.
Background technology
Plant milk extract is the carrier of modern plants medicine modern technique, is the main raw material of plant amedica preparation.Relatively the toxic side effect of chemicals greatly, easily develop immunity to drugs, plant milk extract has unrivaled inherent advantage.Simultaneously, plant milk extract has many characteristics such as exploitation less investment, with high content of technology, added value is big, the world market is widely used, the market development space is big concurrently.Natural plant extracts is widely used in medicine, healthcare products and makeup at present, and the demand in market is very big.
Valerian (Valeriana officinalis L.) is the perennial fragrant herbaceous plant of Valerianaceae, and its root stock and root contain aldehyde lipid volatile oil such as Bornyl acetate.Its pharmaceutical use is with a long history, and as natural calm analgesics, toxic side effect is little, determined curative effect; The root powder is medicinal, and the essential oil that root and rhizome refine can be made senior spices, makeup, food, healthcare products etc.Nineteen twenty, valerian just is included into American Pharmacopeia, afterwards by the pharmacopeia collection of more than 20 countries such as English, method, moral, day.
China is vast in territory, valerian distributes from eastern and western distributed more widely, 24 kinds have been found at present, originate in southwest, northwest, northeast, valerian extract iridoidate compound is the contained main chemical compositions of Chinese valerian, and Rhizoma et radix valerianae extract performance quality directly depends on purification thing purity and stability thereof.Originally Rhizoma et radix valerianae extract is continued to use the supercritical carbon dioxide extraction method always.The complicated component of extract does not have further to separate monomer component wherein, the unstable properties of product.And the less stable of its major function composition iridoid, decomposition reaction, the active disappearance are easily taken place in extraction, separation and transporting procedures, have a strong impact on the stability and the pharmaceutical use of extract.
Summary of the invention
The technical problem to be solved in the present invention just is: at the technical problem of prior art existence, the invention provides a kind of valerian extract iridoidate compound separating and extracting method, its extract component is simple, medicinal ingredients purity height, stable performance, product total iridoid content reaches 86%.
For solving the problems of the technologies described above, the solution that the present invention proposes is: a kind of valerian extract iridoidate compound is characterized in that: with the valerian coarse crushing, soak with aqueous ethanolic solution then and filter, filtrate decompression is concentrated into rare medicinal extract, and vacuum-drying gets the valerian crude extract again.And not only determined with the aqueous ethanolic solution to be the Rhizoma et radix valerianae extract extraction agent, the extraction yield of iridoidate compound can reach 85.48%.The valerian crude extract is carried out purifying, behind the water suspendible that above-mentioned valerian crude extract adding is an amount of, cross macroporous adsorbent resin, it is not muddy to be eluted to elutriant with less water earlier, discards, and uses ethanol elution again, collects elutriant, and the concentrating under reduced pressure drying obtains Rhizoma et radix valerianae extract.
Described a kind of valerian extract iridoidate compound, it is characterized in that: aqueous ethanolic solution is selected 72% (v/v) ethanol for use for the Rhizoma et radix valerianae extract extraction agent, solid-to-liquid ratio is 1: 12, extract three times at 65 ℃, each 60min, described a kind of valerian extract iridoidate compound, it is characterized in that: in every 1mL valerian purification thing solution, add ethanolic soln 2.2mL, E-reagent 1.6mL, Glacial acetic acid 0.5mL is at 66 ℃ of 12min that develop the color down, normal temperature cooling 10min, compared with prior art, advantage of the present invention just is:
1, the every performance index of product of the present invention are qualified, solved Rhizoma et radix valerianae extract complicated component in the past well, and medicinal ingredients purity is low, the problem of unstable properties, product purity, stability Rhizoma et radix valerianae extract more in the past improves a lot, and product total iridoid content reaches 85.9%.
2, decomposition reaction and active the disappearance easily take place in the present invention in its extraction separation, have adopted the stability of utilizing the protection of inert gas technology to solve iridoidate compound well, have solved the Application and Development technical barrier.
Embodiment
A kind of valerian extract iridoidate compound of the present invention with fresh or dried valerian coarse crushing, soaks with aqueous ethanolic solution or refluxing extraction 1-3 time then, and filtration is concentrated into rare medicinal extract with filtrate decompression, and vacuum-drying gets the valerian crude extract again.And not only determined with the aqueous ethanolic solution to be the Rhizoma et radix valerianae extract extraction agent, and mainly selected 72% (v/v) ethanol for use, solid-to-liquid ratio is 1: 12, extract three times at 65 ℃, and each 60min, the extraction yield of iridoidate compound can reach 85.48%.
The valerian crude extract is carried out purifying: after above-mentioned valerian crude extract is added an amount of water suspendible, cross macroporous adsorbent resin, it is not muddy to be eluted to elutriant with less water earlier, discard, use 50%～60% concentration ethanol wash-out again, collect elutriant, the concentrating under reduced pressure drying obtains Rhizoma et radix valerianae extract.At normal temperatures, the absorption property that macroporous adsorbent resin extracts compound to valerian is good, and ethanol that can enough 50～60% can be with Rhizoma et radix valerianae extract wash-out from the resin.
Prepare highly purified valerian extract iridoidate compound fast with preparative liquid chromatography.Determined that improved paradimethy laminobenzaldehyde spectrophotometry valerian extract iridoidate compound extracts experiment condition, in every 1mL valerian purification thing solution, add ethanolic soln 2.2mL, E-reagent 1.6mL, Glacial acetic acid 0.5mL, at 66 ℃ of 12min that develop the color down, normal temperature cooling 10min is with the content of spectrophotometry in 594nm place detection iridoidate compound; Determined the chromatogram working conditions of iridoid, sesquiterpene, alkaloid and flavones in the hplc simultaneous determination valerian simultaneously.In purification process, adopt the RPLC post, investigated in the moving phase ethanol volume fraction, acid additives and pH value the influence of chromatogram separating effect.
Claims (3)
1, a kind of valerian extract iridoidate compound is characterized in that: with the valerian coarse crushing, soak with aqueous ethanolic solution then and filter, filtrate decompression is concentrated into rare medicinal extract, vacuum-drying gets the valerian crude extract again.And not only determined with the aqueous ethanolic solution to be the Rhizoma et radix valerianae extract extraction agent, the extraction yield of iridoidate compound can reach 85.48%.The valerian crude extract is carried out purifying, behind the water suspendible that above-mentioned valerian crude extract adding is an amount of, cross macroporous adsorbent resin, it is not muddy to be eluted to elutriant with less water earlier, discards, and uses ethanol elution again, collects elutriant, and the concentrating under reduced pressure drying obtains Rhizoma et radix valerianae extract.
2, described a kind of valerian extract iridoidate compound is characterized in that: aqueous ethanolic solution is selected 72% (v/v) ethanol for use for the Rhizoma et radix valerianae extract extraction agent, and solid-to-liquid ratio is 1: 12, extract three times at 65 ℃, and each 60min,
3, described a kind of valerian extract iridoidate compound is characterized in that: in every 1mL valerian purification thing solution, add ethanolic soln 2.2mL, and E-reagent 1.6mL, Glacial acetic acid 0.5mL is at 66 ℃ of 12min that develop the color down, normal temperature cooling 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100353588A CN101348489A (en) | 2007-07-16 | 2007-07-16 | Valerian extract iridoidate compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100353588A CN101348489A (en) | 2007-07-16 | 2007-07-16 | Valerian extract iridoidate compound |
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| Publication Number | Publication Date |
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| CN101348489A true CN101348489A (en) | 2009-01-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2007100353588A Pending CN101348489A (en) | 2007-07-16 | 2007-07-16 | Valerian extract iridoidate compound |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102973618A (en) * | 2012-12-26 | 2013-03-20 | 江口县苗药生物科技有限公司 | Method for extracting valerenic acid from valeriana fauriei |
| CN103965157A (en) * | 2014-04-30 | 2014-08-06 | 武汉联合药业有限责任公司 | Preparation method for sesquiterpene lactone compound |
| CN106148011A (en) * | 2016-07-20 | 2016-11-23 | 湖北中烟工业有限责任公司 | The preparation method of a kind of cigarette radix valerianae extract and the application in Medicated cigarette thereof |
| CN113768996A (en) * | 2021-10-29 | 2021-12-10 | 美益添生物医药(武汉)有限公司 | Application of valerian in preparation of product for promoting proliferation of intestinal beneficial bacteria |
-
2007
- 2007-07-16 CN CNA2007100353588A patent/CN101348489A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102973618A (en) * | 2012-12-26 | 2013-03-20 | 江口县苗药生物科技有限公司 | Method for extracting valerenic acid from valeriana fauriei |
| CN102973618B (en) * | 2012-12-26 | 2014-12-10 | 江口县苗药生物科技有限公司 | Method for extracting valerenic acid from valeriana fauriei |
| CN103965157A (en) * | 2014-04-30 | 2014-08-06 | 武汉联合药业有限责任公司 | Preparation method for sesquiterpene lactone compound |
| CN103965157B (en) * | 2014-04-30 | 2016-03-16 | 武汉联合药业有限责任公司 | A kind of preparation method of sesterterpene lactone compound |
| CN106148011A (en) * | 2016-07-20 | 2016-11-23 | 湖北中烟工业有限责任公司 | The preparation method of a kind of cigarette radix valerianae extract and the application in Medicated cigarette thereof |
| CN113768996A (en) * | 2021-10-29 | 2021-12-10 | 美益添生物医药(武汉)有限公司 | Application of valerian in preparation of product for promoting proliferation of intestinal beneficial bacteria |
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Open date: 20090121 |