CN101339170A - Ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound - Google Patents
Ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound Download PDFInfo
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- CN101339170A CN101339170A CN 200810060707 CN200810060707A CN101339170A CN 101339170 A CN101339170 A CN 101339170A CN 200810060707 CN200810060707 CN 200810060707 CN 200810060707 A CN200810060707 A CN 200810060707A CN 101339170 A CN101339170 A CN 101339170A
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- sudan red
- analog compound
- high efficiency
- phase chromatography
- ultraviolet detection
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Abstract
The invention provides a method for detecting 5 sudan reds which has low detection limit, wide linear range and better recovery rate; the method comprises the following steps: the extraction of ionic liquid is extracted from a sample before detection; then direct sampling is carried out, and separation is carried out by a high performance liquid chromatogram and a C18 column; the peak out condition is good, detection is carried out by an ultraviolet detector after separation and therefore better detection result can be obtained; the method can be used for detecting the matters which contain sudan red possibly such as foods and food additives, etc.
Description
Technical field
The invention provides a kind of ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound that utilizes, with the ionic liquid for preparing in advance sudan red analog compound is extracted earlier during mensuration, separating the back with the C18 post then detects with UV-detector, this method can improve the detectability of mensuration greatly, linear wide ranges of while, the recovery is good, can be applicable to the various materials that food, food additives etc. may use sudan red analog.
Background technology
At present, the general use of tonyred detection high performance liquid chromatograph is joined UV-detector and is carried out separation detection, but detectability is low inadequately, can not be detected to the sudan red analog compound that contains that content is lower, the recovery is also undesirable in addition, causes the accuracy of measurement result not high.
Summary of the invention
The object of the invention provides a kind of new detection method, and this method is with the ionic liquid for preparing in advance sample to be extracted before mensuration, directly analyzes after the extraction, can obtain the lower detectability and the recovery preferably.This method is measured easy, and the accuracy height can be widely used in all kinds of food that may add sudan red analog compound and food additives etc.
Still the report that does not have similar detection method both at home and abroad.
Description of drawings
Fig. 1 ion liquid extraction high efficiency liquid phase standard colors spectrogram (2 μ g/mL).
Be labeled as among Fig. 1:
The 1-para red; The 2-Sudan red 1; 3-Sudan red 1 I; The 4-Sudan red III; 5-Sudan red 1 V.
Fig. 2 is the chromatogram of certain food additives
Be labeled as among Fig. 2:
The 2-Sudan red 1; 3-Sudan red 1 I
Embodiment
Embodiment
Concrete steps are as follows:
1, ionic liquid 1-octyl group-3-methylimidazole hexafluorophosphate ([[C
8Mim] [PF
6]) synthetic: raw material 1-methylimidazole and 1-chlorine normal octane are by the ratio mixing of 1: 1.2 (amount of substance), then at 80 ℃ of following backflow 24h.After finishing, reaction tells the ionic liquid of lower floor while hot with separating funnel; With a spot of ethyl acetate washing ionic liquid 3～5 times, then at 75 ℃ of vacuum drying 12h, take out after being cooled to room temperature, obtain product chlorination 1-octyl group-3-methylimidazole ([C
8Mim] Cl).
With [C
8Mim] Cl is dissolved in the low amounts of water, adds the Potassium Hexafluorophosphate solid, stirs 12h at normal temperatures, tells lower floor's ionic liquid after the layering, with the low amounts of water washing, drips the AgNO of 1mol/L in washing ion liquid water
3No white precipitate produces behind the aqueous solution number droplet, shows Cl wherein
-By Ex-all.Product obtains final product [C at 70 ℃ of vacuum drying 12h
8Mim] [PF
6].
2, ion liquid abstraction: (1) takes by weighing chilli powder 0.2g, ionic liquid [C
8Mim] [PF
6] ultrasonic 30 minutes of 0.5ml, filter back sample introduction, sample size 20 μ L liters with the needle-based filtrator.(2) chilli oil or food additives: the 0.5mL sample, ionic liquid [C8mim] [PF6] 2mL magnetic agitation 40min filters the back sample introduction, sample size 20 μ L.Measure wavelength and be set to 506nm, chilli powder 0.2g, ultrasonic 30 minutes of ionic liquid [C8mim] [PF6] 0.5mL filters back sample introduction, sample size 20 microlitres with the needle-based filtrator.(2) chilli oil or food additives: 0.5mL sample, ionic liquid [C
8Mim] [PF
6] 2ml magnetic agitation 40min, filter the back sample introduction.Sample size 20 μ L.
3, the selection of moving phase: test the proportioning of different moving phase, be defined as at last: 85% methyl alcohol, 0-8min,, 10-11min increases methyl alcohol to 100%, keeps 100% methyl alcohol to 25min, 25-26min gets back to methyl alcohol and reduces to 85%, separation four class tonyreds and para red compound that can be successful.
Claims (3)
1. one kind is utilized the ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound, its method is to prepare ionic liquid earlier, join then in the material to be determined and extract, extraction back direct injected is in the C18 post, select suitable moving phase to separate, detect four kinds of tonyreds and a kind of para red material of mensuration low concentration that can be successful again with UV-detector.
2. the method for ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound according to claim 1 is characterized in that, sudan red analog compound can pass through ion liquid abstraction, the efficient height of extraction, and the recovery is good, and detectability is low.
This method also can be applied to can by from liquid extraction classes of compounds or analyze or improve sensitivity and improve detectability with similar scheme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810060707 CN101339170A (en) | 2008-04-25 | 2008-04-25 | Ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810060707 CN101339170A (en) | 2008-04-25 | 2008-04-25 | Ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101339170A true CN101339170A (en) | 2009-01-07 |
Family
ID=40213288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810060707 Pending CN101339170A (en) | 2008-04-25 | 2008-04-25 | Ion liquid extraction high efficiency liquid phase chromatography ultraviolet detection for sudan red analog compound |
Country Status (1)
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175793A (en) * | 2010-12-22 | 2011-09-07 | 晨光生物科技集团股份有限公司 | Method for detecting content of tonyred in plastic product |
| CN102432735A (en) * | 2011-08-30 | 2012-05-02 | 嘉兴学院 | Preparation method of magnetic molecularly imprinted polymer for separating/purifying sudan I |
| CN105277500A (en) * | 2015-12-08 | 2016-01-27 | 哈尔滨幻石科技发展有限公司 | Sudan red ultraviolet curing and detecting device and method |
| CN106370494A (en) * | 2016-10-12 | 2017-02-01 | 林圣灼 | Method for extracting and separating coumarin by utilizing [BuPy(Butyl Pyridine)] PF6 |
| CN107091897A (en) * | 2017-06-02 | 2017-08-25 | 杨大伟 | A kind of method of tonyred in ionic liquid aqueous two-phase extraction HPLC methods detection capsicum |
-
2008
- 2008-04-25 CN CN 200810060707 patent/CN101339170A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175793A (en) * | 2010-12-22 | 2011-09-07 | 晨光生物科技集团股份有限公司 | Method for detecting content of tonyred in plastic product |
| CN102175793B (en) * | 2010-12-22 | 2012-10-03 | 晨光生物科技集团股份有限公司 | Method for detecting content of tonyred in plastic product |
| CN102432735A (en) * | 2011-08-30 | 2012-05-02 | 嘉兴学院 | Preparation method of magnetic molecularly imprinted polymer for separating/purifying sudan I |
| CN105277500A (en) * | 2015-12-08 | 2016-01-27 | 哈尔滨幻石科技发展有限公司 | Sudan red ultraviolet curing and detecting device and method |
| CN106370494A (en) * | 2016-10-12 | 2017-02-01 | 林圣灼 | Method for extracting and separating coumarin by utilizing [BuPy(Butyl Pyridine)] PF6 |
| CN107091897A (en) * | 2017-06-02 | 2017-08-25 | 杨大伟 | A kind of method of tonyred in ionic liquid aqueous two-phase extraction HPLC methods detection capsicum |
| CN107091897B (en) * | 2017-06-02 | 2019-09-13 | 上海博焱检测技术服务有限公司 | A kind of method that ionic liquid aqueous two-phase extraction-HPLC method detects tonyred in capsicum |
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| C06 | Publication | ||
| PB01 | Publication | ||
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Open date: 20090107 |