CN101338285B - Method for producing D(-)-tartaric acid and salts thereof and microbe strains used in the method - Google Patents
Method for producing D(-)-tartaric acid and salts thereof and microbe strains used in the method Download PDFInfo
- Publication number
- CN101338285B CN101338285B CN2007101809070A CN200710180907A CN101338285B CN 101338285 B CN101338285 B CN 101338285B CN 2007101809070 A CN2007101809070 A CN 2007101809070A CN 200710180907 A CN200710180907 A CN 200710180907A CN 101338285 B CN101338285 B CN 101338285B
- Authority
- CN
- China
- Prior art keywords
- salt
- tartrate
- cis
- seq
- succinic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention relates to a microorganism which can convert a cis-epoxysuccinate or salt thereof into a D (-)-tartaric acid. The invention also relates to a method for utilizing the microorganism to manufacture the D(-)-tartaric acid or the salt thereof. Further more, the invention also relates to the usage of the microorganism for producing the D (-)-tartaric acid.
Description
Technical field
The present invention relates to a kind of new production D (-)-tartrate or the method for its salt, and the used microorganism that cis-form epoxy succinic acid or its salt can be converted into D (-)-tartrate or its salt in this method.Particularly, the present invention relates to utilize the method for described microorganisms producing D (-)-tartrate or its salt.Further, the invention still further relates to the purposes of described microorganism in producing D (-)-tartrate or its salt.
Background technology
D (-)-tartrate [(2S, 3S)-2,3-dihydroxy butane-1,4-dicarboxylic acid] seldom exist at occurring in nature, use mainly as chirality synthetic chiral source and resolving agent at pharmaceutical industry.At present D (-)-tartaric demand is just in cumulative year after year, presses for to expand the scale of production, and improves the quality of products, international market demand domestic to satisfy.In the past, D (-)-tartaric production method mainly is to adopt chemical resolution method that DL-tartrate is split as D (-)-tartrate and L (+)-tartrate, perhaps utilizes peculiar microorganism the L (+) in the DL-tartrate-tartrate is exhausted and to be got.At present, adopting the biotransformation method of cis-Epoxysuccinic acid hydratase is the developing direction of D (-)-tartrate production technology, its principle is to be that raw material adds water and obtains maleic acid solution with the MALEIC ANHYDRIDE, be that catalyzer makes maleic acid and hydroperoxidation make cis-form epoxy succinic acid with wolframic acid or tungstate again, utilize the cis-Epoxysuccinic acid hydratase of specified microorganisms that cis-form epoxy succinic acid is hydrolyzed to D (-)-tartrate.There is the microorganism that appears in the newspapers mainly to have the false unit cell of Japanese Patent JP 1996-245497 report to belong to the Alcaligenes (Alcaligenes sp.) of (Pseudomonas sp.), Japanese Patent JP 1975-145586 and JP 2000-14391A report and the Bordetella 1-3 of document FEMS Microbiol Lett 267,214 (2007) reports etc. with D (-)-tartrate lytic enzyme.
Microorganism strains of the present invention is that the inventor separates a new bacterial strain Bordetella BK-52 who obtains from rural area soil, this bacterial strain can be converted into D (-)-tartrate or its salt with cis-form epoxy succinic acid or its salt, has satisfied the demand of domestic, the ever-increasing D in world market (-)-tartrate or its salt.
Summary of the invention
The inventor separates from rural area soil and has obtained a kind of new microorganism strains, and it can be converted into D (-)-tartrate or its salt with cis-form epoxy succinic acid or its salt.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms preservation center on June 11st, 2007, and preserving number is CGMCC No.2075.
One of purpose of the present invention provides a kind of microorganism that cis-form epoxy succinic acid or its salt can be converted into D (-)-tartrate or its salt.
Another object of the present invention provides a kind of microorganism with 16SrDNA sequence consistent with SEQ ID NO:1 sequence.
Microorganism provided by the invention is Bordetella BK-52 (Bordetella sp.BK-52).
Microorganism provided by the invention is the Bordetella with preserving number CGMCC No.2075.
The present invention also provides the nucleotide sequence with SEQ ID NO:2 and SEQ ID NO:3.
The present invention also provides the sequence that has homology with SEQ ID NO:1-3, and homology is preferably at least 50%, and preferably at least 60%, preferably at least 70%, preferably at least 80%, be preferably at least 90%, preferably at least 95%, more preferably at least 99% homology.
The present invention also provides a kind of nucleotide sequence, and it comprises the sequence that is selected among the SEQ ID NO:1-3.
The present invention also provides has the sequence that is selected among the SEQ ID NO:1-3, wherein one or more, preferred 1-15, preferred 1-10, preferred 1-5 modified sequence that Nucleotide is substituted, deletes and/or add and obtains.
Further, the invention still further relates to the purposes of microorganism of the present invention in producing D (-)-tartrate or its salt.
The invention still further relates to mutant or mutation and the biology culture thereof of described microorganism, described microbial mutation body or mutation and biology culture thereof can be converted into D (-)-tartrate or its salt with cis-form epoxy succinic acid or its salt.
Cis-form epoxy succinic acid salt involved in the present invention is the salt that cis-form epoxy succinic acid and various positively charged ion form, and comprises and is not limited only to cis-form epoxy succinic acid ammonium, cis-form epoxy succinic acid potassium, cis-form epoxy succinic acid sodium and cis-form epoxy succinic acid calcium etc.
Other purpose of the present invention sees among the detailed description of the present invention.
The substratum that the present invention utilized can be the substratum of this area routine.Described cultivation can be carried out under the conventional or known condition of this area.For example, can in laboratory or industrial fermentation jar, in suitable substratum and under the suitable condition, come culturing cell by shake-flask culture, small-scale or large scale fermentation (comprising successive, batch-wise, feed supplement-batch-wise fermentation).In comprising the proper nutrition substratum of carbon source and nitrogenous source and inorganic salt, cultivate with methods known in the art.
The composition of the substratum that the present invention is used and content thereof are such as following:
Seed culture medium: glucose 1%, peptone 0.5%, extractum carnis 0.5%, NaCl 1%, and pH 7.5.121 ℃ of sterilization 20min.
Produce the enzyme substratum: glucose 1%, peptone 0.5%, extractum carnis 0.5%, cis-form epoxy succinic acid disodium 1.0%, pH7.5,121 ℃ of sterilization 20min.
Suitable medium can obtain from suppliers, perhaps can prepare with methods known in the art.Be applicable to substratum of the present invention be not limited to recited above those.Incubation time in bacteriological incubator and temperature can be undertaken by the incubation time and the temperature of this area routine.
For the method for strain identification, this is well-known to those skilled in the art.Such as the category judgement method of classics, chemical classification, genetic classification method or the like.Strain identification method of the present invention, employing be the 16SrDNA sequence analysis that development in recent years is got up, its authenticate technology route comprises: the extracting of (1) microbial genome; (2) primer design is synthetic; (3) pcr amplification; (4) PCR product purification; (5) order-checking; (6) online BLAST analyzes.
The method of production D (-)-tartrate of the present invention and salt thereof can utilize microorganism of the present invention to adopt conventional step, device and condition to carry out.
The present invention uses the method for the bacterial characteristics of this area evaluation bacterial strain commonly used and comes Identifying micro-organisms CGMCC No.2075, and its bacterial characteristics is preferably following one or more: Gram-negative; Thalli morphology is short and small shaft-like or spherical (as shown in Figure 1); No gemma.
The accompanying drawing summary
The electron-microscope scanning aspect graph of accompanying drawing 1:CGMCC No.2075 bacterial strain;
The 16S rDNA sequence of accompanying drawing 2:CGMCC No.2075 bacterial strain (SEQ ID NO:1);
The 16S rDNA sequencer map of accompanying drawing 3:CGMCC No.2075 bacterial strain;
The 16S rDNA sequence B last analytical results of accompanying drawing 4:CGMCC No.2075 bacterial strain.
Embodiment
Mode below by embodiment further specifies the present invention.The following examples only are for illustrative purposes, and are not to limit the scope of the invention.
One, the extracting of genomic dna
Used test kit is VIOGENE Genomic DNA Extraction Kit.Concrete extraction steps is as follows:
1, will be in inoculum centrifugal 10min under 5,000 * g of logarithmic phase, collecting precipitation makes that the bacterium number reaches about 1 * 10 in the precipitation
9
2, precipitation is resuspended in 200 μ l N,O-Diacetylmuramidase reaction soln (20mmol/L Tris-HCl, pH8.0; 2mmol/L EDTA; The 20mg/ml N,O-Diacetylmuramidase) in, and in 37 ℃ of following incubation 30min.
3, add 20 μ l Proteinase Ks and 200 μ l EX Buffer in sample, rapidly mixing.
4,60 ℃ of following incubation 30min are with the cracking bacterial cell, and every 5min comes biased sample by vortex or inversion between incubation period.
5, with 10mM Tris-HCl (pH9.0) and ddH
2O is used for the DNA wash-out in 70 ℃ of following preheatings.
6, add in the sample that 210 μ l dehydrated alcohols obtain in the step 3 and the vortex mixing.
7, B/T genomic dna micro-column is placed on the collection tube, the said mixture total number is moved in the post, centrifugal (6,000 * g) 2min place micro-column new collection tube then.
8,0.5ml WS Buffer is added in the above-mentioned new collection tube, centrifugal (6,000 * g) 2min discard effluent liquid.Repeat the washing of twice realization of aforesaid operations to micro-column.
9, again with micro-column at full speed centrifugal 2 min to remove residual ethanol.
10, micro-column is placed the collection tube of new 1.5ml.With the elutriant eluted dna of 200 μ l through preheating.
11. with the upright 1-5min of post, centrifugal then 1-2min is with eluted dna.
12. the DNA of wash-out is stored down in 4 ℃ or-20 ℃.
Two, pcr amplification
1, the employed primer of pcr amplification process is:
10F：5’AGA GTT TGA TCC TGG CTC AG 3’(SEQ ID NO：2)；
1500R：5’GGT TAC CTT GTTACG ACT T 3’(SEQ ID NO：3)。
2, reagent
Ampli Taq Gold (5U/ μ l), 10 * PCR Buffer II (no MgCl
2), MgCl
2Solution (25mmol/L), TOYOBO dNTPs (being 2mmol/L).
3, PCR reaction system
The PCR reaction system comprises: the extractive DNA of 2 μ l, 2.5 μ l, 10 * PCR Buffer II, 2 μ l MgCl
2Solution, 2.5 μ l dNTPs, 2 μ l Primer, 0.25 μ l Ampli Taq Gold adds water and is settled to 25 μ l.
4, reaction conditions:
96 ℃ 5min
72℃ 5min
4 ℃ ∞
To carry out purifying and order-checking evaluation according to following program through the PCR product of said procedure gained.
Three, the purifying of PCR product
1, material
Marker is the DNA Marker DL2000 of Takara company.
The rubber tapping test kit is that the quick glue of Type B miniprep dna fragment of vast Tyke, Beijing biological gene technology limited liability company reclaims test kit.
2, purification step
(1) cuts off the agar sugar that contains DNA, make it as far as possible little, place the 1.5ml centrifuge tube.
(2) ratio that adds 700 μ l sol solutionses in per 100 μ l agaroses adds sol solutions, puts incubation 10min in 50 ℃ of water-baths, and the agar sugar is dissolved fully.Every 2min puts upside down mixing once.
(3) agarose solution after will dissolving moves into adsorption column, centrifugal 30s.Discard liquid in the collection tube, adsorption column is put into collection tube.
(4) add 500 μ l rinsing liquids, centrifugal 30s in the adsorption column.Discard liquid in the collection tube, adsorption column is put into collection tube, centrifugal 1min.
(5) adsorption column is placed a clean 1.5ml centrifuge tube, after adsorption film central authorities added 30 μ l elutriants, leave standstill 1min, centrifugal 1min was stored in-20 ℃ with 1.5ml centrifuge tube (DNA).
Four, order-checking
1, sequencing reaction step:
(1) according to sample panel sample and primer (SEQ ID NO:4-6) that needs react are arranged.
Seq forward：5’GAGC GGAT AACA ATTT CACA CAGG 3’(SEQ IDNO：4)
Seq reverse：5’CGCC AGGG TTTT CCCA GTCA CGAC 3’(SEQ IDNO：5)
Seq internal：5’CAGC AGCC GCGG TAAT AC 3’(SEQ ID NO：6)
(2) get 8 PCR reaction tubess, on 96 orifice plates, arrange.
(3) add 1-3 μ l template DNA in PCR reaction tubes bottom.The band brightness that the usage quantity of PCR product template is seen by agarose electrophoresis is determined.
(4) replenish volume to 12 μ l with 5% aqueous glycerin solution.
(5) add 4 μ l 3.2pM sequencing primers;
(6) add 4 μ l BigDye terminator;
(7) go up the PCR instrument, general cycling condition is as follows:
96℃，2min
4℃， ∞
(8) product is carried out alcohol secondary sedimentation purifying:
I. add 25 μ l sodium-acetate/ethanolic solns (2.5ml 3mol/L sodium-acetate+37.5ml ethanol) in each reaction tubes, behind the mixing at SORVALL
RRT-7 desk centrifuge (U.S. Sorvall company) last 4 ℃ with the centrifugal 30min of 4000r/min; Be inverted 96 orifice plates, it is centrifugal to fill up clean thieving paper, shuts down when being not more than 800r/min;
II. in every pipe, add 70% and freeze alcohol (20 ℃ of preservations) 50 or 100 μ l, the centrifugal 15min of 4000r/min; Be inverted 96 orifice plates, it is centrifugal to fill up clean thieving paper, shuts down when being not more than 800r/min;
III. repeating II goes on foot 1 time;
IV. will precipitate good DNA product and put into 25 μ l deionized formamides (ABI company).
(9) utilize 3700 DNA analysis instrument (PE Applied Biosystems company) to carry out sequential analysis, the sequence results (shown in A, B, C in the accompanying drawing 3) that obtains in 3 sequencing reactions is spliced, obtain complete sequence collection of illustrative plates (shown in D in the accompanying drawing 3).
2, utilize the Blast program, spliced 16S rDNA complete sequence and Genbank nucleic acid sequence data storehouse are compared, obtain result as shown in Figure 4.
The genome of the microorganism strains that the present invention is obtained carries out aforesaid DNA extracting, pcr amplification 16S rDNA, PCR product purification, order-checking, utilize Blast program and GenBank database to compare, the result shows that the 16S rDNA sequence of Bordetella sp.BK-52 is than the long 13kb of Bordetella sp.1-3, have 4 base pairs inequality in the 16S rDNA sequence that two bacterial strains are complementary, therefore the homology of two bacterial strains is at most 99%; All reach 97% with the homology of Bordetella sp.JC15, Bordetella sp.F2, Bordetella sp.E3.This shows that the microorganism strains that the present invention obtains is for having the new subspecies of the active Bordetella of cis-Epoxysuccinic acid hydratase (Bordetella), called after Bordetella BK-52 (Bordetella sp.BK-52).This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms preservation center on June 11st, 2007, and preserving number is CGMCC No.2075.
The inclined-plane of cultivating the above-mentioned Bordetella CGMCC No.2075 of 24h is inoculated in the 250ml Erlenmeyer flask that the 30ml liquid seed culture medium is housed, 30 ℃ of shaking culture 24h, drawing the above-mentioned seed liquor of 10ml is inoculated in the 1000ml triangular flask that 200ml product enzyme substratum is housed, 30 ℃ of shaking culture 24h, in triangular flask, add cis-form epoxy succinic acid disodium 10g gradually, after 72h shaking culture and reaction, add CaCl
2The aqueous solution filters and water washing and precipitating, obtains 11.5g four water calcium tartrates, refining through sulfuric acid solution, zwitterion exchange column again, concentrate, crystallization, separation and oven dry obtain D (-)-tartrate 6.4g.This D (-)-tartaric specific rotatory power is by analysis
Content is 99.7%.
The inclined-plane of cultivating the above-mentioned Bordetella CGMCC No.2075 of 24h is inoculated in the 250ml triangular flask that the 30ml liquid seed culture medium is housed, 30 ℃ of shaking culture 24h, drawing the above-mentioned seed liquor of 10ml is inoculated in the 1000ml triangular flask that 200ml product enzyme substratum is housed, 30 ℃ of shaking culture 24h, in triangular flask, add cis-form epoxy succinic acid calcium 10g, after 100h shaking culture and reaction, filter and water washing and precipitating, obtain 11.1g four water calcium tartrates, again through sulfuric acid solution, the zwitterion exchange column is refining, concentrate, crystallization, separation and oven dry obtain D (-)-tartrate 6.2g, and this D (-)-tartaric specific rotatory power is by analysis
Content is 99.6%.
Embodiment 4 utilizes the cis-form epoxy succinic acid disodium and produces D (-)-tartrate by the Bordetella CGMCC No.2075 strain cell of the present invention of к-carrageenin embedding
The Bordetella CGMCC No.2075 inclined-plane of cultivating 24h is inoculated in the 250ml triangular flask that the 30ml liquid seed culture medium is housed, 30 ℃ of shaking culture 24h, draw the above-mentioned seed liquor of 10ml and be inoculated in the 1000ml triangular flask that 200ml product enzyme substratum is housed 30 ℃ of shaking culture 24h.Centrifugal collection thalline is with к-being fixed of carrageenin embedding Bordetella CGMCC No.2075 biological catalyst 100g.This 100g immobilization Bordetella CGMCC No.2075 biological catalyst is put into 1000ml 1.0mol/L cis-form epoxy succinic acid two sodium solutions, behind 37 ℃ of reaction 72h, the filtered and recycled biological catalyst.Add CaCl in the filtered liquid
2The aqueous solution, through fully stirring after-filtration, washing precipitation gets 193g D (-)-calcium tartrate.With this D (-)-calcium tartrate add sulfuric acid solution, zwitterion exchange column refining, concentrate, crystallization, separation and oven dry obtain D (-)-tartrate 106g, this D (-)-tartaric specific rotatory power is by analysis
Content is 99.6%.
The immobilized biocatalyst that reclaims is put into 1000ml 1.0mol/L cis-form epoxy succinic acid two sodium solutions again, behind 37 ℃ of reaction 72h, the filtered and recycled biological catalyst.Add CaCl in the filtered liquid
2The aqueous solution after fully stirring, filters and washing precipitation, obtains 198g D (-)-calcium tartrate.This D (-)-calcium tartrate is refining with sulfuric acid solution, zwitterion exchange column, concentrate, crystallization, separation and oven dry obtain D (-)-tartrate 109g, this D (-)-tartaric specific rotatory power is by analysis
Content is 99.7%.
This immobilized biocatalyst can use repeatedly.
In summary, to have cis-form epoxy succinic acid and salt hydrolysis thereof be the characteristic of D (-)-tartrate and salt thereof to the present invention Bordetella CGMCC No.2075 of separating acquisition.
The biomaterial preservation
Clause according to budapest treaty, following biomaterial has been preserved in (No.3 A, DaTun Road, Chaoyang District, BeiJing City, China Committee for Culture Collection of Microorganisms common micro-organisms preservation center, Institute of Microorganism, Academia Sinica, 100101), its preservation data is as follows:
| Preservation thing (Deposit) | Preservation registration number | Preservation date |
| Bordetella BK-52 (Bordetella sp.BK-52) | CGMCC No.2075 | On June 11st, 2007 |
Be understandable that top description only is an example of the present invention, thereby the scope that claim of the present invention is protected limits with particular disclosed herein not merely.Any equivalent embodiments will be deemed to be within the scope of the present invention.In fact, according to the description of front, it all will be possible for those skilled in the art that the present invention is carried out relevant modifications and variations, thereby this modifications and variations also will drop within the scope of the appended claim of the present invention.
Sequence table
＜110〉Hangzhou Baojing Biology Chemical Co., Ltd
＜120〉microorganism strains of production D (-)-tartrate and salt thereof
<130>C07P0191
<160>6
<170>PatentIn version 3.1
<210>1
<211>1450
<212>DNA
＜213〉Bordetella bacterial classification (Bordetella sp.) CMGCC No.2075
<400>1
attgacgcta gcgggatgct ttacacatgc aagtcgaacg gcagcacgga cttcggtctg 60
gtggcgagtg gcgaacgggt gagtaatata tcggaacgtg cccagtagcg ggggataact 120
acgcgaaagc gtagctaata ccgcatacgt cctatgggag aaagcggggg accttcgggc 180
ctcgcactat tggagcggcc gatatcggat tagctagttg gtggggtaaa ggctcaccaa 240
ggcgacgatc cgtagctggt ttgagaggac gaccagccac actgggactg agacacggcc 300
cagactccta cgggaggcag cagtggggaa ttttggacaa tgggggcaac cctgatccag 360
ccatcccgcg tgtgcgatga aggccttcgg gttgtaaagc acttttggca ggaaagaaac 420
ggttccggat aatacctgga gcaactgacg gtacctgcag aataagcacc ggctaactac 480
gtgccagcag ccgcggtaat acgtagggtg caagcgttaa tcggaattac tgggcgtaaa 540
gcgtgcgcag gcggttcgga aagaaagatg tgaaatccca gagcttaact ttggaactgc 600
atttttaact accgagctag agtgtgtcag agggaggtgg aattccgcgt gtagcagtga 660
aatgcgtaga tatgcggagg aacaccaatg gcgaaggcag cctcctggga taacactgac 720
gctcatgcac gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgcccta 780
aacgatgtca actagctgtt ggggccttcg ggccttagta gcgcagctaa cgcgtgaagt 840
tgaccgcctg gggagtacgg tcgcaagatt aaaactcaaa ggaattgacg gggacccgca 900
caagcggtgg atgatgtgga ttaattcgat gcaacgcgaa aaaccttacc tacccttgac 960
atgtctagaa ggctaaagag atttggctgt gctcgcaaga gaactggaac acaggtgctg 1020
catggctgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080
cttgtcatta gttgctacgc aagagcactc taatgagact gccggtgaca aaccggagga 1140
aggtggggat gacgtcaagt cctcatggcc cttatgggta gggcttcaca cgtcatacaa 1200
tggtcgggac agagggttgc caagccgcga ggtggagcta atctcataaa cccgatcgta 1260
gtccggatcg cagtctgcaa ctcgactgcg tgaagtcgga atcgctagta atcgcggatc 1320
agcatgtcgc ggtgaatacg ttcccgggtc ttgtacacac cgcccgtcac accatgggag 1380
tgggttttac cagaagtagt tagcctaacc gtaaggaggg cgattaccac ggtaggattc 1440
atgactgggg 1450
<210>2
<211>20
<212>DNA
＜213〉primer
<400>2
agagtttgat cctggctcag 20
<210>3
<211>19
<212>DNA
＜213〉primer
<400>3
<210>4
<211>24
<212>DNA
＜213〉primer
<400>4
gagcggataa caatttcaca cagg 24
<210>5
<211>24
<212>DNA
＜213〉primer
<400>5
cgccagggtt ttcccagtca cgac 24
<210>6
<211>18
<212>DNA
＜213〉primer
<400>6
cagcagccgc ggtaatac 18
Claims (6)
1. cis-form epoxy succinic acid or its salt can be converted into the microorganism strains of D (-)-tartrate or its salt, it is Bordetella BK-52 (Bordetella sp.BK-52), and this microorganism is characterised in that it has and is selected from following one or more features: Gram-negative; It is short and small shaft-like or spherical that thalli morphology is; No gemma, with SEQ ID NO:2 and SEQ ID NO:3 is primer, pcr amplification 16SrDNA, with SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 is primer, adopt ABI 3700DNA analyser to check order to reclaiming product, the sequence that records is the sequence shown in the SEQ ID NO:1, and it is the Bordetella BK-52 with preserving number CGMCC No.2075.
2. method of producing D (-)-tartrate or its salt, this method comprise utilizes the described microorganism strains of claim 1 that cis-form epoxy succinic acid or its salt catalytic hydrolysis are got.
3. the method described in the claim 2, wherein cis-form epoxy succinic acid salt is cis-form epoxy succinic acid and the formed salt of various positively charged ion.
4. the purposes of the microorganism of claim 1 in producing D (-)-tartrate or its salt.
5. the culture of the described microorganism of claim 1, it can be converted into D (-)-tartrate or its salt with cis-form epoxy succinic acid or its salt.
6. biological catalyst, it comprises the described microorganism of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101809070A CN101338285B (en) | 2007-10-09 | 2007-10-09 | Method for producing D(-)-tartaric acid and salts thereof and microbe strains used in the method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101809070A CN101338285B (en) | 2007-10-09 | 2007-10-09 | Method for producing D(-)-tartaric acid and salts thereof and microbe strains used in the method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101338285A CN101338285A (en) | 2009-01-07 |
| CN101338285B true CN101338285B (en) | 2011-07-20 |
Family
ID=40212455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101809070A Active CN101338285B (en) | 2007-10-09 | 2007-10-09 | Method for producing D(-)-tartaric acid and salts thereof and microbe strains used in the method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101338285B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101654663B (en) * | 2009-03-24 | 2012-06-06 | 浙江大学 | Bordetella strain and application thereof |
| CN105087677A (en) * | 2014-04-21 | 2015-11-25 | 怀来县长城生物化学工程有限公司 | D-(-)-tartaric acid clean production process based on bipolar membrane electroosmosis technology |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081714A (en) * | 1992-07-30 | 1994-02-09 | 浙江大学 | Utilize the tartaric method of microorganisms producing d- |
-
2007
- 2007-10-09 CN CN2007101809070A patent/CN101338285B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1081714A (en) * | 1992-07-30 | 1994-02-09 | 浙江大学 | Utilize the tartaric method of microorganisms producing d- |
Non-Patent Citations (2)
| Title |
|---|
| Xia Li等.Isolation and characterization of a new bacterium capable ofbiotransforming cis-epoxysuccinic acid to D(-)-tartaric acid.FEMS Microbiol Lett267.2006,摘要，第216页右栏第1-4段、图1，第215页右栏第1-3段、倒数第2-3段. * |
| Zhang,J.等.GENBANK:EU000383.NCBI.2007,全文. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101338285A (en) | 2009-01-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Voordouw et al. | Identification of distinct communities of sulfate-reducing bacteria in oil fields by reverse sample genome probing | |
| Weidner et al. | Diversity of uncultured microorganisms associated with the seagrass Halophila stipulacea estimated by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes | |
| Bovio et al. | Preliminary analysis of Chloroflexi populations in full‐scale UASB methanogenic reactors | |
| CN103981119B (en) | The application of oily sludge petrochina efficient degrading bacteria and bacterium group | |
| Yang et al. | Wastewater treatment systems harbor specific and diverse yeast communities | |
| Saisa-Ard et al. | Characterization and phylogenetic analysis of biosurfactant-producing bacteria isolated from palm oil contaminated soils. | |
| CN100584939C (en) | Method for producing L(+)-tartaric acid and salt thereof, and microorganism bacterial strain used in the method | |
| CN101338285B (en) | Method for producing D(-)-tartaric acid and salts thereof and microbe strains used in the method | |
| CN110055191B (en) | Yersinia bailii L M-W separated from bottom mud of paint spraying wastewater and application thereof | |
| CN109609388B (en) | Aspergillus niger and application thereof | |
| CN114317302B (en) | Black and odorous water body remediation microbial inoculum and application thereof | |
| CN109554303B (en) | Microbacterium aurantiacus and preparation and application thereof | |
| Malimas et al. | Gluconobacter kondonii sp. nov., an acetic acid bacterium in the α-Proteobacteria | |
| CN106282069B (en) | A kind of pair coccus and the application in sewage purification | |
| CN111621437B (en) | Otter escherichia coli LM-DK separated from oxidation pond of pig farm and application thereof | |
| CN111808770B (en) | Air bacillus for degrading crude oil and application thereof | |
| CN110004085B (en) | Brevibacterium LM-R separated from paint spraying wastewater bottom sediment and application thereof | |
| CN115074272B (en) | Biological desulfurization bacillus aryabhattai and application thereof | |
| CN100400646C (en) | Chlorine resisting strain No.1 and screening process thereof | |
| CN110358687A (en) | One plant of gibberella for producing D pantoic acid lactone hydrolase and application, fermentation process | |
| CN112980749B (en) | Rhodococcus erythropolis oxydans ethane and propane and application thereof | |
| CN116121099B (en) | Mangrove bacillus and application thereof and straw cellulose degradation method | |
| CN102643918A (en) | Method for specifically separating sphingomonas yanoikuyae by combining streptomycin resistance surface plate based on special primer polymerase chain reaction (PRC) | |
| CN114214260B (en) | Halomonas LYX-3 separated from soil of smelting plant and application thereof | |
| CN114292771B (en) | Pseudomonas stutzeri WZY-3-1 for efficiently degrading anthrone and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: HANGZHOU BIOKING BIOLOGICAL CO., LTD. Free format text: FORMER NAME: HANGZHOU BIOKING BIOTECHNOLOGY CO., LTD. Owner name: HANGZHOU BIOKING BIOTECHNOLOGY CO., LTD. Free format text: FORMER NAME: HANGZHOU BIOKING BIOCHEMICAL ENGINEERING CO.,LTD |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 311106 Industrial Zone, Tangqi Town, Yuhang District, Zhejiang, Hangzhou Patentee after: HANGZHOU BIOKING BIOCHEMICAL ENGINEERING Co.,Ltd. Address before: 311106 Industrial Zone, Tangqi Town, Yuhang District, Zhejiang, Hangzhou Patentee before: Hangzhou crystal biological Polytron Technologies Inc. Address after: 311106 Industrial Zone, Tangqi Town, Yuhang District, Zhejiang, Hangzhou Patentee after: Hangzhou crystal biological Polytron Technologies Inc. Address before: 311106 Industrial Zone, Tangqi Town, Yuhang District, Zhejiang, Hangzhou Patentee before: HANGZHOU BIOKING BIOCHEMICAL ENGINEERING Co.,Ltd. |