CN101337900A - Method of synthesizing camptothecin-relating compounds - Google Patents
Method of synthesizing camptothecin-relating compounds Download PDFInfo
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Abstract
In order to effectively provide initial compound CPT of irinotecan hydrochloride and various camptothecinum derivatives through a practical total synthesis, the invention provides a method for effectively preparing 2'-amino-5'-(benzeneboronic)ethyl ketone homologous to AB-ring part of the framework of camptothecinum (CPT) and tricyclic ketone homologous to CDE-ring part and stably supplying CPT and the derivatives thereof.
Description
The application be the applying date be on February 21st, 2002, Chinese patent application number be 02805323.0, denomination of invention divides an application for " synthetic method of camptothecin-relating compounds ".
Technical field
The present invention relates to the synthetic method of camptothecin-relating compounds.In more detail, the present invention relates to and the synthetic relevant intermediates preparation of camptothecin and the purposes of described intermediate, and relate to the complete synthesis of camptothecin with anti-tumor activity.
Background technology
Isolating camptothecine (hereinafter referred to as CPT) such as the bark of the camplotheca acuminata (Camptotheca acuminata) that originates in from China, root, fruit, leaf are the five rings alkaloids, known its by suppressing the synthetic anti-tumor activity that shows of nucleic acid.On the other hand, in camptothecin derivative, reported side effect (the cancer とization Learn Treatment method 17 of inducing diarrhoea etc., p115-120,1990), also have the problem that causes Alimentary infringement, in order to reduce purposes such as toxicity, reinforced effects, after tested various derivatives.
The present inventor has reported the compound that alleviates than such CPT toxicity, water-soluble semi synthesis of derivatives 7-ethyl-10-[4-(1-piperidino-(1-position only))-1-piperidino-(1-position only) of CPT] carbonyl oxygen base camptothecine hydrochloride trihydrate (CPT-11 hereinafter referred to as), now it is widely used as antitumour drug (general name: U 101440E).
Camptothecin as CPT-11 can obtain by the CPT that obtains from natural materials is carried out chemically modified.
Yet,,,, can expect that the CPT supply of sufficient quantity is still very difficult though taked the measure of raw material supplies such as trees planting along with the demand of useful derivative CPT-11 etc. increases because the amount of the CPT that obtains from natural materialss such as starting material camplotheca acuminatas is very low.Though also tested by complete synthesis method for making, present situation is still not reach practicability.
As by complete synthesis method for making, knownly represent by following reaction formula, Shen, W. etc. through aminophenyl ethyl ketone and tricycle kentones
Method (the J.Org.Chem.1993 of reaction, 58,611-617 " Concise Total Syntheses of dl-Camptothecinand Related Anticancer Drugs. "), but this method still exists step miscellaneous, and yield is not high yet and problem that can only the synthesising racemation body.
On the other hand; though Curran; D.P. wait method (Chem.Eur.J.1998 by the cascade free radical cyclization that uses aryl isonitrile that following reaction formula represents and iodo pyridone; 4; 67-83 " A General Synthetic Approach to the (20S)-CamptothecinFamily of Antitumor Agents by a Regiocontrolled Cascade RadicalCyclization of Aryl Isonitriles. ") carried out complete synthesis; but the yield that proposes cyclization is insufficient, must carry out the problem of the deprotection of protecting group after the cyclisation.
In addition, though according to following mechanism, above-mentioned Curran, D.P. etc. have synthesized the intermediate 4-iodo-2-methoxyl group-6-trimethyl silyl pyridine-3-formaldehyde in tricycle kentones part synthetic of CPT class,
But this method makes dangerous the rising because needing a large amount of industrial inflammable t-BuLi of use, and to require temperature of reaction be-78 ℃ reaction, therefore can not enlarge in batches.In addition, owing to need in the reaction system integral body to carry out complicated temperature control, so it is not the reaction system of industrial practicality.
Summary of the invention
The objective of the invention is the complete synthesis initial compounds CPT that U 101440E and various camptothecin derivatives are provided effectively by practicality, and the camptothecin of U 101440E synthetic important intermediate 7-ethyl-10-hydroxycamptothecine etc.Especially, the objective of the invention is the synthetic respectively intermediate and the intermediate that is equivalent to the CDE loop section that is equivalent to the AB loop section of camptothecin skeleton, and further with the synthetic camptothecin of these intermediates.
Embodiment
The inventor has carried out broad research to these situations, about the AB loop section, with compound (a) (5-hydroxyl-2-nitrobenzaldehyde):
As initial substance, 2 '-amino-the 5 '-hydroxypropiophenone that has prepared the AB loop section that is equivalent to the CPT skeleton effectively, thereby the means of CPT and derivative thereof have been found stably to provide, about the CDE loop section, with compound (k) (2-methoxyl group-6-trimethyl silyl pyridine (MTP)):
(TMS represents trimethyl silyl in the formula, the Me represent methylidene)
As initial substance, prepared the tricycle kentones of the CDE loop section that is equivalent to the CPT skeleton effectively, thereby found the stable means that CPT and derivative thereof are provided, by the suitable combination of these means, set up total synthesis method, thereby finished the present invention without the CPT class of natural materials.
That is to say, the present invention relates to basis
The preparation method of 2 ' of the AB loop section that is equivalent to the CPT skeleton of (R represents protecting group in the formula)-amino-5 '-hydroxypropiophenone, and preparation method's the total synthesis method of CPT class that has made up the tricycle kentones of the CDE loop section that is equivalent to the CPT skeleton, the preparation method of described tricycle kentones comprises based on known synthetic route Curran route (Josien, H; Ko, S.B.; Bom, D.; Curran, D.P.Chem.Eur.J.1998,4 67-83) and Pharmacia ﹠amp; Upjohn route (P﹠amp hereinafter referred to as; The U route; Heneger, K.E.; Ashford, S, W.; Baughman, T.A.; Sih, J.C.; Gu, R.L.J.Org.Chem.1997,62,6588-6597) and the synthetic route set up
(in the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl, and Pr represents propyl group,
tBu represents the tertiary butyl) improve and optimize, particularly (compound (v)) synthesizes 3-formyl radical-4-iodo-2-methoxyl group-6-trimethyl silyl pyridine (compound (l)) by 2-methoxyl group-6-trimethyl silyl pyridine (compound (k)) or 3-methylol-4-iodo-2-methoxyl group-6-trimethyl silyl pyridine.In addition, because (v) be the by product that generates from the process of the synthetic 3-(2-butylene oxygen methyl) of compound (l)-4-iodo-2-methoxyl group-6-trimethyl silyl pyridine (compound (m)), compound in said synthesis route (l) is described compound below.
In more detail, the present invention relates to be used for the preparation method of 2 ' of synthetic camptothecin-amino-5 '-hydroxypropiophenone, wherein from compound (a):
Generate compound (b):
Generate compound (c) from compound (b):
Generate (d) from compound (c):
Generate compound (e) from compound (d):
It is characterized in that R may pass through catalytic reduction and the protecting group of deprotection.
The invention still further relates to aforesaid method, it is characterized in that may be by catalytic reduction the protecting group of deprotection be benzyl.
In addition, the present invention relates to aforesaid method, it is characterized in that described method comprises one or more following steps that are selected from:
(1) compound (a), benzyl reagent and alkali are mixed, and in solvent this mixture of heated and stirred and obtain the step of compound (b);
(2) under atmosphere of inert gases, in compound (b), drip Grignard reagent and obtain the step of compound (c);
(3) compound (c) and oxygenant are mixed, and stir and the step of acquisition compound (d); And
(4) catalytic reduction compound (d) and obtain the step of compound (e).
In addition, the present invention relates to aforesaid method, wherein the solvent in the step (1) is a dimethyl formamide.
The invention still further relates to aforesaid method, wherein the Grignard reagent in the step (2) is a vinyl bromination magnesium.
In addition, the present invention relates to aforesaid method, wherein the oxygenant in the step (3) is Jones reagent, Manganse Dioxide or TEMPO-(2,2,6,6-tetramethyl piperidine-1-oxygen)-clorox.
The invention still further relates to the compound of molecular formula (c ') expression:
(Bn represents benzyl in the formula).
In addition, the present invention relates to the compound of molecular formula (d '):
(Bn represents in the formula).
The invention still further relates to the preparation method who is used for 2 ' of synthetic camptothecin-amino-5 '-hydroxypropiophenone, wherein from compound (a):
The generation compound (c "):
Generate compound (d ") from compound (c "):
Generate compound (e) from compound (d "):
In addition, the present invention relates to aforesaid method, it is characterized in that described method comprises one or more following steps that are selected from:
(1) under atmosphere of inert gases, in compound (a), drips Grignard reagent and obtain the step of compound (c ");
(2) compound (C ") and oxygenant are mixed, and stir and obtain the step of compound (d ");
(3) catalytic reduction compound (d ") and obtain the step of compound (e).
The invention still further relates to aforesaid method, wherein the Grignard reagent in the step (1) is a vinyl bromination magnesium.
In addition, the present invention relates to aforesaid method, wherein the oxygenant in the step (2) is Jones reagent, Manganse Dioxide or TEMPO-clorox.
The invention still further relates to 2 '-amino-the 5 '-hydroxypropiophenone that obtains by preceding method and be used to prepare the purposes of camptothecin.
In addition, the present invention relates to the preparation method of camptothecin, described method comprises makes 2 '-amino-5 '-hydroxypropiophenone and the tricycle kentones reaction that obtains by preceding method.
The invention still further relates to the preparation method of the tricycle kentones that is used for synthetic camptothecin, wherein from compound (k):
(in the formula, TMS represents trimethyl silyl, and Me represents methyl), or compound (v):
(in the formula, TMS represents trimethyl silyl, and Me represents methyl) generates compound (l):
(in the formula, TMS represents trimethyl silyl, and Me represents methyl), generate compound (m) from compound (l):
(in the formula, TMS represents trimethyl silyl, and Me represents methyl), generate compound (n) from compound (m):
(in the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl), generate compound (o) from compound (n):
(in the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl), generate compound (p) from compound (o):
(in the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl), generate compound (q) from compound (p):
(in the formula, Me represents methyl, and Et represents ethyl), generate compound (r) from compound (q):
(in the formula, Me represents methyl, and Et represents ethyl, and Pr represents propyl group), generate compound (s) from compound (r):
(in the formula, Et represents ethyl, and Pr represents propyl group), generate compound (t) from compound (s):
(in the formula, Et represents ethyl,
tBu represents the tertiary butyl), generate compound (h) from compound (t):
(in the formula, Et represents ethyl), it is characterized in that described method comprises one or more following steps that are selected from:
(1) compound (k), lithiation reagent, formylation reagent and iodination reagent are mixed and the step of acquisition compound (l);
(2) compound (l), crotyl alcohol, triethyl-silicane and acid are mixed, and under without the condition of solvent this mixture of reaction and obtain the step of compound (m);
(3) by-product compounds in the step (2) (is v) mixed and the step of acquisition compound (l) with oxygenant and alkali;
(4) compound (m), palladium catalyst, alkali and phase-transfer catalyst are mixed, and in solvent this mixture of boiling reflux and obtain the step of compound (n);
(5) compound (n), osmium catalyst, co-oxidants, alkali and unsymmetrical reagent are mixed and the step of acquisition compound (o);
(6) compound (o), alkali and iodine are mixed, and in alcohol-water mixed liquid this mixture of boiling reflux and obtain the step of compound (p);
(7) compound (p) and desilylation iodination reagent are mixed and the step of acquisition compound (q);
(8) by compound (q), palladium catalyst and alkali are mixed, and under the carbon monoxide atmosphere in n-propyl alcohol this mixture of reaction and obtain the step of compound (r);
(9), and at room temperature react this mixture and obtain the step of compound (s) with compound (r) and demethylation reagent mix;
(10) compound of reaction (s) and obtain the step of compound (t) in the presence of tert-butyl acrylate and alkali.
In addition, the present invention relates to aforesaid method, wherein the lithiation reagent in the step (1) is a n-Butyl Lithium.
The invention still further relates to aforesaid method, wherein the temperature of reaction in the step (1) is-30～-40 ℃ a constant temperature.
In addition, the present invention relates to aforesaid method, wherein the oxygenant in the step (3) is the TEMPO-clorox.
The invention still further relates to aforesaid method, wherein the alkali in the step (4) is salt of wormwood or N, the N-diisopropylethylamine.
In addition, the present invention relates to aforesaid method, wherein the solvent in the step (4) is tetrahydrofuran (THF) or diisopropyl ether-acetonitrile-water mixed solution.
The invention still further relates to aforesaid method, wherein the osmium catalyst in the step (5) is the sour potassium of osmium (VI).
In addition, the present invention relates to aforesaid method, wherein in step (6), for compound (o), iodine is 4 equivalents.
The invention still further relates to aforesaid method, wherein the desilylation iodination reagent in the step (7) is iodo-trifluoroacetic acid silver or N-chlorosuccinimide-sodium iodide.
In addition, the present invention relates to aforesaid method, wherein by purification step compound (q) is carried out chemical purification, described purification step comprises:
The reaction product that will obtain by the step that generates compound (q) from compound (p) adds the alkaline aqueous solution and the step that stirs;
After adding organic solvent and stirring, the step of removing organic layer; And
Make water layer become acidity, and with the step of organic solvent extraction.
The invention still further relates to aforesaid method, wherein alkaline aqueous solution is an aqueous sodium hydroxide solution.
In addition, the present invention relates to aforesaid method, wherein organic solvent is a chloroform.
The invention still further relates to aforesaid method, wherein by purification step compound (q) is carried out the optics purifying, described purification step comprises:
After the reaction product that will obtain by the step that generates compound (q) from compound (p) is dissolved in the high polar solvent, the step of lamination low polar solvent; And
Behind the filtering precipitate, under reduced pressure filtrate is concentrated into dried step.
In addition, the present invention relates to aforesaid method, wherein high polar solvent is a chloroform.
The invention still further relates to aforesaid method, wherein low polar solvent is a normal hexane.
In addition, the present invention relates to aforesaid method, wherein the alkali in the step (10) is salt of wormwood.
The invention still further relates to the purposes that is used to prepare camptothecin by the tricycle kentones of aforesaid method acquisition.
In addition, the present invention relates to the preparation method of camptothecin, described method comprises makes tricycle kentones and 2 '-amino-the 5 '-hydroxypropiophenone reaction that is obtained by aforesaid method.
The invention still further relates to aforesaid method, wherein 2 '-amino-5 '-hydroxypropiophenone is 2 '-amino-the 5 '-hydroxypropiophenone that is obtained by aforesaid method.
In addition, the present invention relates to aforesaid method, wherein tricycle kentones and 2 '-amino-5 '-hydroxypropiophenone is mixed, and under atmosphere of inert gases, react this mixture.
The present invention becomes possibility, and makes the complete synthesis practicability of CPT become possibility by 2 '-amino-the 5 '-hydroxypropiophenone that adopts these key elements to make effective preparation be equivalent to the AB loop section of CPT skeleton.In addition, about the midbody compound among the preparation method of the present invention (c ') and compound (d '), still do not have the report of their synthetic, so they are useful new compounds in CPT is synthetic.
The present invention is by adopting these key elements, also makes the asymmetric synthesis method of compound of the skeleton of the CDE loop section (tricycle kentones part) that practicably has in the CPT skeleton become possibility.
Synthetic about the AB loop section of CPT skeleton (2 '-amino-5 '-hydroxypropiophenone), the preparation method of 2 '-amino-5 '-hydroxypropiophenone comprises one or more following steps:
(1) from the synthetic 5-benzyloxy of 5-hydroxyl-2-nitrobenzaldehyde (compound (a))-2-nitrobenzaldehyde (compound (b ')) step;
(2) from the synthetic 1-(5-benzyloxy-2-nitrophenols) of compound (b ')-2-propenyl-1-alcohol (compound (c ')) step;
(3) from the synthetic 1-(5-benzyloxy-2-nitrophenols) of compound (c ')-2-propenyl-1-ketone (compound (d ')) step; And
(4) from compound (d ') Synthetic 2 '-step of amino-5 '-hydroxypropiophenone (compound (e)).
Typical synthetic route can be expressed as following synthetic route:
(in the formula, R express possibility the protecting group of deprotection) by catalytic reduction.
In the present invention; as long as R may pass through catalytic reduction and the protecting group of deprotection; there is no particular limitation to it; typical example is benzyl, methoxybenzyl, 2; benzylic ethers such as 6-dimethyl benzyl, 4-nitrobenzyl are protecting group, and benzyl carbonic ether such as carbobenzoxy-(Cbz) is protecting group, especially; from the angle of reagent cost, use benzyl to suit.
In addition,, can use,,, perhaps contain the natural matter of compound (a) from the material of separation such as various natural materialss, purifying from the material of similar compounds chemical transformation by currently known methods synthetic material as starter compound (a).Also can use the commercial reagent.
Be described more specifically above-mentioned steps (1)～(4) below.
In step (1), (a) is dissolved or suspended in the solvent with compound, adds benzyl reagent and alkali, and heating is also stirred and acquisition compound (b).
As solvent, can use N, dinethylformamide (DMF), methyl-sulphoxide, chloroform, acetonitrile, ethanol, water etc., especially, from solvability and reactive angle, DMF is preferred.
For compound (a), the usage quantity of DMF can be preferably the scope of 3～20 times of amounts for more than 3 times or 3 times.
As benzyl reagent, can use any normally used benzyl reagent aptly.The specific examples of benzyl reagent is benzyl chloride, bromotoluene, benzyl iodide, phenyldiazomethane, dimethyl benzyl etc., particularly can use benzyl chloride aptly.
The usage quantity of benzyl reagent can be modulated aptly according to reagent, for example under the situation of using benzyl chloride, for compound (a), can use 1～5 equivalent, preferred 1～2 equivalent.
As alkali, can use any normally used alkali aptly.The specific examples of alkali is salt of wormwood, yellow soda ash, cesium carbonate, sodium hydroxide, potassium hydroxide etc., especially, can use salt of wormwood aptly.
The usage quantity of alkali can be modulated aptly according to reagent, for example under the situation of using salt of wormwood, for compound (a), uses 1～10 equivalent, preferred 1～4 equivalent.
Heating temperature especially, is preferred in 60～80 ℃ scope in 60～100 ℃ scope.
In addition, the reaction times especially, is preferred in 1～20 hour scope in 0.5～24 hour scope.
In step (2), obtain compound (c) by under atmosphere of inert gases, in compound (b), dripping Grignard reagent.
As rare gas element, can use the rare gas class of any argon gas, helium, neon, krypton gas, xenon, radon gas etc., the perhaps low gas of nitrogen isoreactivity, especially, and from the angle of cost, preferred helium and nitrogen.
As Grignard reagent, can use any normally used Grignard reagent aptly.The specific examples of Grignard reagent is vinyl bromination magnesium, vinyl chlorination magnesium, vinyl iodate magnesium etc., especially, can use vinyl bromination magnesium aptly.
The usage quantity of Grignard reagent can be modulated aptly according to reagent, for example if vinyl bromination magnesium is 1～2 equivalent for compound (b), and preferred 1～1.5 equivalent.
Grignard reagent can be added drop-wise in the solution of compound (b), also can be on the contrary the drips of solution of compound (b) be added in the Grignard reagent, but in order to reduce reduced form by product (compound (f) hereinafter referred to as)
(in the formula, R express possibility the protecting group of by catalytic reduction deprotection) preferably is added drop-wise to Grignard reagent in the solution of compound (b).
The quantity of solvent of using about when reaction, for example if tetrahydrofuran (THF) (hereinafter referred to as THF), its amount can be 10～100 times of amounts, especially, in order to reduce the generation of alcohol, is preferably 50～100 times of amounts.
Temperature of reaction preferably is no more than 10 ℃, especially, in order to reduce the generation of alcohol, is preferably-78～-40 ℃.
Reaction times is 0.1～3 hour, especially, is preferably 0.5～1 hour.
In step (3), by compound (c) and oxygenant are mixed, and stir and obtain compound (d).
Oxygenant can use any normally used oxygenant aptly.The example of this oxygenant is Manganse Dioxide, Dess-Martin reagent (Dess-Martin Periodinane), Jones reagent (Na
2Cr
2O
7/ H
2SO
4), PCC, PDC, DMSO/ oxalyl chloride/triethylamine (Swern oxidation), TEMPO-clorox etc., especially, preferably use Manganse Dioxide, Dess-Martin reagent, Jones reagent and TEMPO-clorox.
These oxygenants preferably just prepare before use, for example if Manganse Dioxide, can use before use the Manganse Dioxide from potassium permanganate and manganous sulfate preparation aptly.
The usage quantity of oxygenant can be according to reagent and modulation aptly, and for example if Manganse Dioxide, for compound (c), its amount is 2～50 times of amounts, preferred 4～10 times of amounts.
As solvent, can use for example chloroform, methylene dichloride, ethyl acetate, benzene, toluene etc. aptly, especially, be preferably chloroform and methylene dichloride.
The usage quantity of solvent is 5～50 times of amounts, is preferably 10～20 times of amounts.
Reaction times is 1～48 hour, especially, is preferably 1～18 hour.
In step (4), can obtain compound (e) by catalytic reduction compound (d).
As catalyst for reduction, can use palladium-carbon, palladium hydroxide-carbon, rhodium-aluminum oxide etc. aptly, especially, be preferably palladium-carbon and palladium hydroxide-carbon.
For compound (d), the usage quantity of catalyst for reduction is 0.01～0.5 equivalent, is preferably 0.05～0.2 equivalent.
As solvent, can use any normally used solvent aptly, but from deliquescent angle, ethyl acetate is preferred.
For compound (d), the usage quantity of solvent is 5～50 times of amounts, is preferably 10～20 times of amounts.
Reaction times in 0.1～24 hour scope, especially, preferably in 1～3 hour scope.
In addition, through above-mentioned steps (1) to step (4) synthetic compound (e) not, and can be from compound (a):
The generation compound (c "):
Generate compound (d ") from compound (c "):
Can generate compound (e) from compound (d "):
In this synthetic route, can obtain compound (c ") in the compound (a) by under atmosphere of inert gases, Grignard reagent being added drop-wise to.In addition, can be by compound (c ") and oxygenant be mixed, and stir and acquisition compound (d "), can pass through catalytic reduction compound (d ") and obtain compound (e).Here identical in operable Grignard reagent and oxygenant and above-mentioned steps (2) and the step (3).In this synthetic route, owing to, therefore can carry out the synthetic of AB loop section easily without protecting group.
In addition, camptothecin analogues can be prepared,, for example compound (h) can be used as such tricycle kentones by compound (e) and the tricycle kentones reaction that step (4) or said synthesis route are obtained:
Synthetic about the CDE loop section (tricycle kentones part) of CPT skeleton carries out the preparation of tricycle kentones through following synthetic route.
In the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl, and Pr represents propyl group,
tBu represents the tertiary butyl).
As the initial compounds compound (K) in the said synthesis route, can use by aforesaid Curran route (Josien, H; Ko, S.B.; Bom, D.; Curran, D.P.Chem.Eur.J.1998,4,67-83) synthetic compound from the compound of similar compounds chemical transformation, from the compound of various natural materialss separation, purifying, perhaps contains the natural materials of compound (k) itself.
The preferred synthetic method of the tricycle kentones in the above-mentioned synthetic route comprises one or more in following 12 steps:
(1) the step of synthetic 4-iodo-2-methoxyl group-6-trimethyl silyl-3-pyridylaldehyde (hereinafter referred to as compound (l)) from 2-methoxyl group-6-trimethyl silyl pyridine (hereinafter referred to as compound (k)), use n-Butyl Lithium as alkali, under-30～-40 ℃ constant temperature, react;
(2) the step of synthetic 3-(2-butylene oxygen methyl)-4-iodo-2-methoxyl group-6-trimethyl silyl pyridine (hereinafter referred to as compound (m)), do not use reaction solvent from compound (l);
(3) (the step hereinafter referred to as compound (v)) synthetic compound (l), using the TEMPO-clorox from 3-methylol-4-iodo-2-methoxyl group-6-trimethyl silyl pyridine as oxygenant;
(4) synthesizing 4-ethyl-8-methoxyl group-6-trimethyl silyl-1H-pyrans [3 from compound (m), 4-c] in the step of pyridine (hereinafter referred to as compound (n)), the mixed solution that uses diisopropyl ether, acetonitrile and water is as reaction solvent, and use N, and the N-diisopropylethylamine is as alkali;
(5) synthesizing (S)-4-ethyl-3 from compound (n), 4-dihydro-3 in the step of 4-dihydroxyl-8-methoxyl group-6-trimethyl silyl-1H-pyrans [3,4-c] pyridine (hereinafter referred to as compound (o)), uses the sour potassium of osmium (VI) as osmium catalyst;
(6) from synthetic (the S)-4-ethyl-3 of compound (o), in the step of 4-dihydro-4-hydroxyl-8-methoxyl group-6-trimethyl silyl-3-oxygen-1H-pyrans [3,4-c] pyridine (hereinafter referred to as compound (p)), use 4 normal iodine to carry out boiling reflux;
(7) from synthetic (the S)-4-ethyl-3 of compound (p), in the step of 4-dihydro-4-hydroxyl-6-iodo-8-methoxyl group-3-oxygen-1H-pyrans [3,4-c] pyridine (hereinafter referred to as compound (q)), be used in the N-chlorosuccinimide-sodium iodide in the acetic acid;
(8) in the step of chemical purification compound (q), add alkaline aqueous solution such as aqueous sodium hydroxide solution and make solution be alkalescence, wash with the organic solvent of chloroform etc., then be the tart water layer with organic solvent extractions such as chloroforms;
(9) in the step of optics purifying compounds (q), compound (q) is dissolved in the high polar solvent of chloroform etc., carry out lamination with the low polar solvent of normal hexane etc., filter throw out that obtains and concentrated filtrate;
(10) from synthetic (the S)-4-ethyl-3 of compound (q), in the step of 4-dihydro-4-hydroxyl-8-methoxyl group-3-oxygen-1H-pyrans [3,4-c] pyridine-6-carboxylic acid propyl ester (hereinafter referred to as compound (r)), use palladium as palladium catalyst;
(11) synthesizing (S)-4-ethyl-3,4,7 from compound (r), 8-tetrahydrochysene-4-hydroxyl-3 in the step of 8-dioxy-1H-pyrans [3,4-c] pyridine-6-carboxylic acid propyl ester (hereinafter referred to as compound (s)), at room temperature reacts;
(12) synthesizing (S)-4-ethyl-3,4,8 from compound (s), 10-tetrahydrochysene-4,6-dihydroxyl-3,10-dioxy-1H-pyrans [3,4-f] indolidin-7-carboxylic acid 1, in the step of 1-dimethyl ethyl ester (hereinafter referred to as compound (t)), use salt of wormwood to carry out the Michel addition reaction.
In addition, (13) from (S)-4-ethyl-7,8-dihydro-4-hydroxyl-1H-pyrans [3,4-f] indolidin-3,6,10 (4H)-triketones (hereinafter referred to as compound (h)) and compound (e) obtain by reacting, can obtain SN-38 aptly in the step of SN-38 under atmosphere of inert gases.
Below will be specifically described above-mentioned 13 steps.
In (1), by compound (k) is dissolved in the solvent, add lithiation reagent, formylation reagent and iodination reagent, and stir and acquisition compound (l).
As solvent, can use tetrahydrofuran (THF) (THF), diethyl ether, hexane, heptane etc., especially, from solvability and reactive angle, THF is preferred.
As lithiation reagent, can use any normally used lithiation reagent aptly.The specific examples of lithiation reagent is n-Butyl Lithium, s-butyl lithium, tert-butyl lithium, diisopropylamine lithium (LDA), two (trimethyl silyl) amido lithium (LiHMDS) etc., especially, from operation and reactive angle, can use n-Butyl Lithium aptly.
The usage quantity of lithiation reagent can be modulated aptly according to reagent, and for example under the situation of using n-Butyl Lithium, for compound (k), its consumption is 2～10 equivalents, preferred 2～5 equivalents.
The specific examples of formylation reagent is N-formyl radical-N, N ', and N '-trimethylammonium quadrol, N, dinethylformamides (DMF) etc. are considered follow-up iodination reaction, can use N-formyl radical-N aptly, N ', N '-trimethylammonium quadrol.
About the usage quantity of formylation reagent, for example using N-formyl radical-N, N ', under the situation of N '-trimethylammonium quadrol, for compound (k), it is 1～10 equivalent, is preferably 1～3 equivalent.
As iodination reagent, can use iodine, N-iodosuccinimide (NIS) etc., especially, from cost and reactive angle, iodine is preferred.
For compound (k), the usage quantity of iodination reagent is 1～10 equivalent, is preferably 1～5 equivalent.
Temperature of reaction is preferably-30～-40 ℃ constant temperature in 0～-78 ℃ scope.
In (2), by crotyl alcohol, triethyl-silicane and acid are added in the compound (l), and stir under without the condition of solvent and obtain compound (m).
For compound (l), the usage quantity of crotyl alcohol is 1～10 equivalent, is preferably 2～5 equivalents.
For compound (l), the usage quantity of triethyl-silicane is 1～10 equivalent, is preferably 1～4 equivalent.
As acid, can use trifluoroacetic acid (TFA), sulfuric acid, methylsulfonic acid, hydrochloric acid etc., especially, from reactive angle, TFA is preferred.
About the usage quantity of acid, for example under the situation of TFA, for compound (l), it is 1～15 equivalent, is preferably 5～10 equivalents.
In (3), by the by-product compounds in (2) (v) is dissolved in the solvent, and adds oxygenant and alkali, stir and acquisition compound (l).
As solvent, can use any normally used solvent aptly.As this solvent, can enumerate methylene dichloride, chloroform, acetonitrile, toluene, normal hexane etc., especially, from reactive angle, toluene and normal hexane are preferred.
As oxygenant, can enumerate Manganse Dioxide, Dess-Martin reagent (Dess-MartinPeriodinane), Jones reagent (Na
2Cr
2O
7/ H
2SO
4), PCC, PDC, DMSO-oxalyl chloride-triethylamine (Swern oxidation), TEMPO-hypochlorite, especially, be preferably the TEMPO-hypochlorite, more preferably the TEMPO-clorox.
About the usage quantity of oxygenant, for example under the situation of TEMPO-clorox, (v), TEMPO is 0.001～0.1 equivalent, is preferably 0.005～0.02 equivalent with respect to compound.In addition, the consumption of clorox is 1～5 equivalent, is preferably 1～2 equivalent.
As alkali, can use any normally used alkali aptly.As this alkali, can enumerate sodium bicarbonate, yellow soda ash, salt of wormwood, lime carbonate, sodium hydroxide, calcium hydroxide, triethylamine etc., especially, sodium bicarbonate is preferred.
About the usage quantity of alkali, for example under the situation of sodium bicarbonate, (v), the consumption of sodium bicarbonate is 1～10 equivalent, is preferably 2～4 equivalents with respect to compound.
Temperature of reaction is in-10～30 ℃ scope, and especially, in order to suppress side reaction, it is preferably-10～10 ℃.
Reaction times is in 0.5～10 hour scope, preferably in 0.5～5 hour scope.
In (4), by compound (m) is dissolved in the solvent, add palladium catalyst, alkali and phase-transfer catalyst, boiling reflux and obtain compound (n).
As solvent, can use acetonitrile, tetrahydrofuran (THF) (THF), diisopropyl ether (IPE), diethyl ether, toluene, water etc., especially, from reactive angle, acetonitrile, THF, IPE and water are preferred, more preferably THF or acetonitrile-IPE-water mixed liquid.
As palladium catalyst, can use palladium, tetrakis triphenylphosphine palladium (o), dichloro-two (triphenylphosphine) palladium (II), Palladous chloride etc. aptly, especially, from reactive angle, palladium is preferred.
For compound (m), the usage quantity of palladium catalyst is 0.01～1 equivalent, is preferably 0.05～0.2 equivalent.
As alkali, can use any normally used alkali aptly.The example of this alkali is yellow soda ash, salt of wormwood, lime carbonate, cesium carbonate, triethylamine (TEA), N, and N-diisopropylethylamine (DIPEA), sodium hydroxide, potassium hydroxide etc. especially, can use salt of wormwood and DIPEA aptly.
About the usage quantity of alkali, for example under the situation of DIPEA, for compound (m), its consumption is 1～20 equivalent, is preferably 5～10 equivalents.
As phase-transfer catalyst, can use any normally used quaternary ammonium salt or crown ether aptly, especially, the bromination tetrabutylammonium.
About the usage quantity of phase-transfer catalyst, for example under the situation of bromination tetrabutylammonium, for compound (m), its consumption is 0.1～3 equivalent, is preferably 0.5～1.5 equivalent.
Under the situation of using THF, the reaction times is in 1～20 hour scope, preferably in 4～10 hours scope.Under the situation of using acetonitrile-IPE-water mixed liquid, it is in 0.5～10 hour scope, preferably in 1～5 hour scope.
In (5), by compound (n) is dissolved in alcohol-water mixed liquid, add osmium catalyst, co-oxidants, asymmetric catalyst, alkali and amsacrine, stir and acquisition compound (o).
As alcohol, can enumerate methyl alcohol, ethanol, 1-propyl alcohol, Virahol (IPA), 1-butanols, 2-butanols, the trimethyl carbinol etc., especially, from reactive angle, the trimethyl carbinol is preferred.
As osmium catalyst, can use perosmic anhydride, the sour potassium of osmium (VI) etc. aptly, especially, from the angle of operation, the sour potassium of osmium (VI) is preferred.
For compound (n), the usage quantity of osmium catalyst is 0.001～0.1 equivalent, is preferably 0.002～0.01 equivalent.
As co-oxidants, can use the sour potassium of six cyano group iron (III), 4-methylmorpholine N-oxide compound (NMO) etc. aptly, especially, from reactive angle, the sour potassium of six cyano group iron (III) is preferred.
About the usage quantity of co-oxidants, for example under the situation of the sour potassium of six cyano group iron (III), for compound (n), its consumption is 1～10 equivalent, is preferably 2～5 equivalents.
As asymmetric catalyst, can enumerate (DHQD)
2PYR, (DHQD)
2PHAL, (DHQD)
2AQN etc., especially, from the angle of asymmetric yield, (DHQD)
2PYR is preferred.
About the usage quantity of asymmetric catalyst, for example at (DHQD)
2Under the situation of PYR, for compound (n), its consumption is 0.005～0.1 equivalent, is preferably 0.01～0.05 equivalent.
As alkali, can use yellow soda ash, salt of wormwood, lime carbonate, cesium carbonate, sodium hydroxide, potassium hydroxide etc., especially, from reactive angle, salt of wormwood is preferred.
About the usage quantity of alkali, for example under the situation of salt of wormwood, for compound (n), its consumption is 1～20 equivalent, is preferably 4～10 equivalents.
For compound (n), the usage quantity of amsacrine is 0.1～5 equivalent, is preferably 0.5～2 equivalent.
Temperature of reaction is preferably-10～10 ℃ in-10～30 ℃ scope.
In (6), by compound (o) is dissolved in the solvent, add alkali and iodine, boiling reflux and obtain compound (p).
As solvent, can enumerate methyl alcohol, ethanol, 1-propyl alcohol, Virahol (IPA), water etc., especially, from reactive angle, methanol-water mixture is preferred.
As alkali, can use any normally used alkali aptly.As this alkali, can enumerate yellow soda ash, salt of wormwood, lime carbonate, cesium carbonate, sodium hydroxide, potassium hydroxide etc., especially, lime carbonate is preferred.
About the usage quantity of alkali, for example under the situation of lime carbonate, for compound (o), its consumption is 1～10 equivalent, is preferably 2～5 equivalents.
With respect to the amount of compound (o), the usage quantity of iodine is 1～10 equivalent, is preferably 3～5 equivalents.
Reaction times in 0.5～20 hour scope, more preferably 1～5 hour.
In (7), by compound (p) is dissolved in the solvent, at the iodo-Silver Trifluoroacetate (hereinafter referred to as I
2-CF
3COOAg) or under N-chlorosuccinimide-sodium iodide (hereinafter referred to as NCS-NaI) react and acquisition compound (q).
As solvent, at I
2-CF
3Under the situation of COOAg, methylene dichloride, tetracol phenixin, chloroform etc. are more suitable, and especially, methylene dichloride is preferred.In addition, under the situation of NCS-NaI, can use acetic acid, acetonitrile etc., especially, from reactive angle, acetic acid is preferred.
About I
2-CF
3The usage quantity of COOAg, for compound (p), I
2Consumption be 1～10 equivalent, be preferably 2～4 equivalents.In addition, CF
3The consumption of COOAg is 1～10 equivalent, is preferably 2～4 equivalents.
About the usage quantity of NCS-NaI, for compound (p), the consumption of NCS is 1～20 equivalent, is preferably 5～8 equivalents.In addition, the consumption of NaI is 1～20 equivalent, is preferably 5～8 equivalents.
Using I
2-CF
3Under the situation of COOAg, the temperature during reaction is 10～60 ℃, is preferably 20～40 ℃.In addition, under the situation of using NCS-NaI, temperature of reaction is 20 a ℃～boiling reflux temperature, is preferably 50～80 ℃.
Reaction times is preferably 15～24 hours in 5～48 hours scope.
In (8), the basic solvents such as aqueous sodium hydroxide solution of compound (q) and for example 0.2N are added, stir and make compound (q) form lactone open loop body (compound (u)):
(in the formula, Me represents methyl, and Et represents ethyl), it is dissolved in the alkaline aqueous solution.When with this solution of organic solvent washing, the material of neutrality～alkalescence shifts in organic layer.Behind the organic layer separatory, make water layer be acid with acid, and reclaim compound (q) with good purity with organic solvent extraction.
The alkali solvent is preferably 0.1～1N in the scope of 0.01～5N.0.2～0.5N more preferably.
As employed alkali, can enumerate potassium hydroxide, calcium hydroxide, sodium hydroxide, salt of wormwood, yellow soda ash etc., especially, sodium hydroxide is preferred.
As organic solvent, can use any normally used organic solvent aptly.As this solvent, can enumerate methylene dichloride, chloroform, ethyl acetate, toluene, diethyl ether, diisopropyl ether etc., especially, methylene dichloride and chloroform are preferred.
As employed acid, can enumerate hydrochloric acid, sulfuric acid, nitric acid, acetic acid, phosphoric acid, trifluoroacetic acid etc., especially, hydrochloric acid is preferred.
In (9), (q) is dissolved in the high polar solvent with compound, uses the low polar solvent lamination, separates out crystallization.Filtering for crystallizing, under reduced pressure concentrated filtrate is to doing.The crystallization that obtains is a racemic modification, and further the compound (q) of optics purifying obtains as residue.
As high polar solvent, can use chloroform, methylene dichloride, ethyl acetate, methyl alcohol, ethanol, propyl alcohol etc., especially, chloroform is preferred.
About employed high polar solvent amount, for example under the situation of chloroform, for 1g compound (q), it is preferably 3～6ml in the scope of 1～10ml.
As low polar solvent, can enumerate normal hexane, normal heptane, diethyl ether etc., especially, normal hexane is preferred.
About high polar solvent: the ratio of low polar solvent, for example at chloroform: under the situation of normal hexane, it is preferably 2: 1～1: 2 in 10: 1～1: 20 scope.
The temperature of crystallization operation preferably is no more than room temperature, more preferably no more than 5 ℃.
In (10), by compound (q) is dissolved in the 1-propyl alcohol, add palladium catalyst and alkali, under the CO (carbon monoxide converter) gas atmosphere, react and acquisition compound (r).
As palladium catalyst, can use palladium, tetrakis triphenylphosphine palladium (o), dichloro-two (triphenylphosphine) palladium, Palladous chloride etc. aptly, especially, from reactive angle, palladium is preferred.
For compound (m), the usage quantity of palladium catalyst is 0.005～0.5 equivalent, is preferably 0.01～0.1 equivalent.
As alkali, can use any normally used alkali aptly.The example of this alkali is yellow soda ash, salt of wormwood, lime carbonate, cesium carbonate, triethylamine (TEA), N, and N-diisopropylethylamine (DIPEA), sodium hydroxide, potassium hydroxide etc. especially, can use salt of wormwood aptly.
About the usage quantity of alkali, for example under the situation of salt of wormwood, for compound (m), its consumption is 1～20 equivalent, is preferably 4～10 equivalents.
Temperature of reaction is in the scope of 20 ℃～boiling reflux temperature, preferably in the scope of 50 ℃～boiling reflux temperature.
In (11), by compound (r) is dissolved in the solvent, add demethylation reagent, at room temperature react and obtain compound (s).
As solvent, can use acetonitrile, chloroform, methylene dichloride, toluene etc., especially, acetonitrile is preferred.
As demethylation reagent, can enumerate chloro trimethyl silane-sodium iodide, iodo trimethyl silane, hydroiodic acid HI, Hydrogen bromide etc., especially, from reactive viewpoint, chloro trimethyl silane-sodium iodide is preferred.
About the usage quantity of demethylation reagent, for example under the situation of chloro trimethyl silane-sodium iodide, for compound (r), its consumption is preferably 2～5 equivalents in 1～10 normal scope.
In (12), by compound (s) is dissolved in the solvent, add alkali, under atmosphere of inert gases, stir.In the mixture that tert-butyl acrylate is dripped as obtains, under rare gas element, stir and acquisition compound (t).
As solvent, can use methyl-sulphoxide (DMSO), N aptly, dinethylformamide (DMF) etc., especially, from reactive angle, DMSO is preferred.
As alkali, can use salt of wormwood, yellow soda ash, sodium hydroxide, potassium hydroxide etc., especially, salt of wormwood is preferred.
About the usage quantity of alkali, for example under the situation of salt of wormwood, for compound (s), it is 1～20 equivalent, is preferably 2～5 equivalents.
As rare gas element, can use rare gas classes such as argon gas, helium, neon, krypton gas, xenon, radon gas, the perhaps low gas of nitrogen isoreactivity, especially, from the angle of cost, argon gas and nitrogen are preferred.
For compound (s), the usage quantity of tert-butyl acrylate is 1～20 equivalent, is preferably 8～12 equivalents.
Temperature of reaction is preferably 40～60 ℃ in 20～80 ℃ scope.
Reaction times is 5～48 hours, and especially, for the decomposition of the compound (t) that prevents to generate, temperature of reaction preferably is no more than 24 hours.
In (13), by compound (h) and compound (e) are dissolved in the solvent, add acid, and under atmosphere of inert gases heated and stirred and obtain SN-38.
As solvent, can use toluene, acetic acid etc. aptly, especially, toluene-acetic acid mixed solution is preferred.
As rare gas element, can use rare gas such as argon gas, helium, neon, krypton gas, xenon, radon gas, the perhaps low gas of nitrogen isoreactivity, especially, from the angle of cost, argon gas and nitrogen are preferred.
As acid, can use toluenesulphonic acids, methylsulfonic acid, trifluoroacetic acid etc., especially, from reactive angle, toluenesulphonic acids is preferred.
About the usage quantity of acid, for example under the situation of toluenesulphonic acids, for 1g compound (h), it is 1～100mg, is preferably 10～30mg.
For compound (h), the usage quantity of compound (e) is 1～3 equivalent, is preferably 1～1.5 equivalent.
Temperature of reaction is in the scope of 50 ℃～boiling reflux temperature, preferably in the scope of 80 ℃～boiling reflux temperature.
The present invention will be described in more detail by the following examples, but the invention is not restricted to these embodiment.
[embodiment 1] compound (b ') synthetic
Bn represents benzyl in the formula.
38.5g (0.230mol) compound (a) is dissolved in 116mL DMF or the acetone, under argon atmosphere, under room temperature, add 33.4g (0.242mol while stirring, 2.1 equivalent) salt of wormwood and 27.8mL (0.242mol, 1.05 equivalents) or 59.95mL (0.461mol, 2 equivalents) benzyl chloride.After the interpolation, 60 ℃ of heating down, and vigorous stirring.Regularly confirm the residual quantity of compound (a), after 20 hours, after the disappearance of affirmation compound (a), suction filtration.
With the solid matter that the washing of 116mL reaction solvent is obtained, merging filtrate and washings under reduced pressure boil off solvent.After boiling off, add 300mL water in residue, after the stirring, the suction filtration throw out is also air-dry.After air-dry, will leach thing and be dissolved in the 170mL ethyl acetate, under agitation it be added in the 1L hexane.The solid that suction filtration is separated out, after the mixed solvent washing with 300mL ethyl acetate-hexane (1: 10), drying under reduced pressure.The amount of benzyl chloride is depended in preparation example 1 and 2 difference.In preparation example 3, acetone is used as reaction solvent.
[table 1]
| Reaction solvent | The benzyl amount of reagent | Reaction times | Separation yield | |
| Preparation example 1 | DMF | 1.05 equivalent | 20 hours | 94％ |
| Preparation example 2 | DMF | 2.00 equivalent | 1 hour | 94％ |
| Preparation example 3 | Acetone | 2.00 equivalent | 18 hours | - |
-: do not reach detectability
As shown in table 1, under the situation of using 1.05 normal benzyl chlorides (preparation example 1), react on end of a period in 20 hours, yield is 94%.On the one hand, under the situation of using 2.00 normal benzyl chlorides (preparation example 2), react on end of a period in 1 hour, yield is 94%.The DMF amount is minimum to be 3 times of amounts, otherwise separates out solid matter in reaction, makes to stir difficulty.On the other hand, when using acetone as reaction solvent, even reflux 18 hours, reaction is not carried out yet.
The HPLC operational condition
Post: Inertsil ODS-2,5 μ m, 4.6mm ID * 250mm (GL Science production)
Near temperature: the constant temperature 40 ℃
Moving phase: water: acetonitrile mixed solution (1: 1)
Flow velocity: 1mL/ minute
Detect wavelength: 220nm
Synthetic (1) of [embodiment 2] compound (c ')
Bn represents benzyl in the formula.
1.0g (3.89mmol) compound (b ') is dissolved among the 20mL THF, under argon atmosphere in 15 minutes Dropwise 5 .84mL (5.84mmol, 1.5 equivalents) vinyl bromination magnesium THF solution (1.0M) while stirring.This moment, the internal temperature of reaction solution was 3～10 ℃.Stir after 1 hour, reaction soln is under agitation joined in the saturated aqueous ammonium chloride solution of 20mL.Then add 20mL ethyl acetate and 4mL hexane, the 3g dried over sodium sulfate is used in each the 20mL washing of the organic layer water of acquisition and saturated aqueous common salt, under reduced pressure boils off solvent, obtains reaction product A.
Under argon atmosphere, while stirring the THF solution of the compound of 20mL such as above-mentioned preparation (b ') was added drop-wise in 15 minutes in the vinyl bromination magnesium THF solution (1.0M) of 5.84mL under ice-cold.This moment, the internal temperature of reaction solution was 3～10 ℃.Stir after 1 hour, reaction soln is under agitation added in the 20mL saturated aqueous ammonium chloride.Then add 20mL ethyl acetate and 4mL hexane, after each the 20mL washing of organic layer water that obtains and saturated aqueous common salt, use the 3g dried over sodium sulfate, under reduced pressure boil off solvent, obtain reaction product B.
Reaction product A that obtains and reaction product B are respectively by silica gel chromatography column purification (ethyl acetate: hexane=1: 20), obtain preparation example 4 from reaction product A, obtain preparation example 5 from reaction product B.
[table 2]
| The yield of compound (c ') | The yield of compound (f) (peak area %) | |
| Preparation example 4 | 84.0％ | 3.5％ |
| Preparation example 5 | 26.8％ | 11.3％ |
As shown in table 2, when in the solution that Grignard reagent is added drop-wise to compound (b '), the generation of by-product compounds (f) is inhibited, and yield has raise 57%.
HPLC operational condition: reference example 1.
Synthetic (2) of [embodiment 3] compound (c ')
1.0g (3.89mmol) compound (b ') is dissolved among 10～100mL THF, under argon atmosphere in 15 minutes Dropwise 5 .84mL (5.84mmol, 1.5 equivalents) vinyl bromination magnesium THF solution (1.0M) while stirring.Stir after 1 hour, reaction soln is under agitation joined in the 20mL saturated aqueous ammonium chloride.Then add 20mL ethyl acetate and 4mL hexane, each the 20mL washing of organic layer water that obtains and saturated aqueous common salt, and use the 3g dried over sodium sulfate, and under reduced pressure boil off solvent, obtain reaction product.
With the reaction product that obtains by with embodiment 2 (preparation example 4 and 5) in same method purifying, obtain preparation example 6～preparation example 9.Preparation example 6 is to react with the quantity of solvent of 20 times of amounts down 20 ℃ of temperature of reaction to obtain, and preparation example 7～9th obtains with the quantity of solvent of 10 times of amounts, 40 times of amounts, 100 times of amounts respectively under 3 ℃.
[table 3]
| Temperature of reaction | Quantity of solvent | The yield (peak area %) of compound (c ') | The yield of compound (f) (peak area %) | |
| Preparation example 4 | 3℃ | 20 times of amounts | 84.0％ | 3.5％ |
| Preparation example 6 | 20℃ | 20 times of amounts | 68.7％ | 4.8％ |
| Preparation example 7 | 3℃ | 10 times of amounts | 81.1％ | 5.7％ |
| Preparation example 8 | 3℃ | 40 times of amounts | 88.6％ | 3.5％ |
| Preparation example 9 | 3℃ | 100 times of amounts | 90.2％ | 2.8％ |
As shown in table 3, by below 10 ℃ or 10 ℃, more preferably to react below 5 ℃ or 5 ℃, the generation of compound (f) is inhibited, and the yield of compound (C ') has also raise 15%.On the other hand, by the quantity of solvent (preparation example 9) of using 100 times of amounts, the generation of compound (f) is inhibited, and yield has also raise 6%.
HPLC operational condition: reference example 1.
Synthetic (1) of [embodiment 4] compound (d ')
Bn represents benzyl in the formula.
(1) preparation of Manganse Dioxide:
Under stirring at room, in 96.0g/600mL (0.607mol) potassium permanganate solution, add 122g/150mL (0.506mol) five anhydrous manganeses and 117mL 40% aqueous sodium hydroxide solution.Stir after 18 hours, suction filtration and water are washed.The air-dry solid matter that obtains obtains 91.2g Manganse Dioxide.
(2) compound (d ') is synthetic
2.00g (7.02mmol) compound (c ') is dissolved in 20mL chloroform, methylene dichloride or the ethyl acetate, under argon atmosphere, add 8.00g (4 times of amounts while stirring under 25 ℃ of conditions, 92.0mol, 13 equivalents) and Manganse Dioxide by method for preparing, and vigorous stirring.After 15 hours, after the affirmation raw material disappears, carry out suction filtration.With the solid matter that the washing of 20mL chloroform is obtained, merging filtrate and washings under reduced pressure boil off solvent.Obtain preparation example 10～12.
[table 4]
| Reaction solvent | Reaction times | Raw material residual | Yield | |
| Preparation example 10 | Chloroform | 15 hours | - | 91％ |
| Preparation example 11 | Methylene dichloride | 3 hours | - | 79％ |
| Preparation example 12 | Ethyl acetate | 24 hours | 8％ | - |
-: do not reach detectability
As shown in table 4, by using chloroform or methylene dichloride, synthesized compound (d ') with high yield as solvent, especially, by using methylene dichloride, the reaction times can shorten more than 3 times or 3 times.On the other hand, under the situation of ethyl acetate, even after 24 hours, still residual have a compound (c ').
Synthetic (2) of [embodiment 5] compound (d ')
7.0g (3.5mmol) compound (c '), toluene (70mL), ethyl acetate (70mL), water (10mL) and 38.3mg (1mol%) TEMPO are mixed, drip 42mL aqueous sodium hypochlorite solution (available chlorine min.5.0% and sodium bicarbonate aqueous solution (7.1g sodium bicarbonate on ice-cold following vigorous stirring limit, 60mL water) (2～6 ℃, 55 minutes).After 5 minutes, raw material also has 0.4% (HPLC, peak area %).With the organic layer separatory, with KI/ aqueous potassium hydrogen sulfate washing (yellow → russet), after saturated aqueous sodium thiosulfate and water washing, under reduced pressure boil off solvent, acquisition 6.4g compound (d ') (yield 91%, purity 92.6%).Get 3.0g with methanol-water (25: 1) recrystallization, obtain 2.3g (purity 95.2% that recrystallization yield 77%, HPLC record).
HPLC operational condition: reference example 1.
Synthesizing of [embodiment 6] compounds (e)
Bn represents benzyl in the formula.
1.84g (6.50mmol) compound (d ') is dissolved in the 37mL ethyl acetate, and under argon atmosphere, ice-cold stirring limit, limit adds 0.69g (0.65mmol, 10mol%) 10% palladium-charcoal.With mixture under atmosphere of hydrogen in 25 ℃ of vigorous stirring, regularly reclaim.Filter the reaction solution that is obtained, boil off filtrate.Obtain preparation example 13～14.
[table 5]
| Reaction times | The yield of compound (e) | The yield of compound (g) (peak area %) | |
| Preparation example 13 | 0.1 hour | 71％ | 14％ |
| Preparation example 14 | 13 hours | 81％ | 0％ |
As shown in table 5, by the reaction more than 13 hours or 13 hours, the generation of by-product compounds (g) is inhibited, and the yield of compound (e) has raise 10%.
The HPLC operational condition
Post: Inertsil ODS-2,5 μ m, 4.6mm ID * 250mm (GL Science production)
Near temperature: the constant temperature 40 ℃
Moving phase: water: acetonitrile mixed solution (1: 1)
Flow velocity: 1mL/ minute
Detect wavelength: 254nm
[embodiment 7] 2 '-amino-5 '-hydroxypropiophenone complete synthesis
The preparation process that below shows 2 '-amino-5 '-hydroxypropiophenone (compound (e)).
(1) compound (b ') is synthetic
1.00g (5.98mmol) compound (a) is dissolved among the 3mL DMF, adds 0.87g (6.28mmol, 2.1 equivalents) salt of wormwood and 0.72mL (6.28mmol, 1.05 equivalents) benzyl chloride while stirring in room temperature under under the argon atmosphere room temperature, enclosing.After the interpolation, in 60 ℃ of heating, and vigorous stirring.Regularly confirm the residual quantity of compound (a), after 20 hours, after the disappearance of affirmation compound (a), carry out suction filtration.Behind the suction filtration, obtain solid matter.
The solid matter that is obtained is washed with 3mL DMF, and merging filtrate and washings under reduced pressure boil off solvent.After boiling off, residue is added in the 100mL water, after the stirring, the suction filtration precipitate, and air-dry it.After air-dry, drying under reduced pressure (1mmHg, 20 ℃), acquisition 1.45g (yield 95%) faint yellow solid compound (b ').
The rerum naturas such as NMR spectrum that below show compound (b ').
Compound (b '): 71～73 ℃ of mp
1H-NMR(400MHz，CDCl
3)：δ5.21(2H，s，PhCH
2O)，7.21(1H，dd，J＝2.8，9.3Hz)，7.35-7.44(6H，m)，8.16(1H，d，J＝9.3Hz)，10.48(1H，s，CHO)
IR(KBr)：1250，1333，1514，1589，1697cm
-1
EI-MS：m/z 257(M
+)。
(2) compound (c ') is synthetic
1.0g (3.89mmol) compound (b ') is dissolved among the 20mL THF, under argon atmosphere in the 15 minutes ice-cold stirring of inner edge limit Dropwise 5 .84mL (5.84mmol, 1.5 equivalents) vinyl bromination magnesium THF solution (1.0M).This moment, the internal temperature of reaction solution was 3～10 ℃.Stir after 1 hour, reaction soln is under agitation added in the saturated ammonium chloride solution.Then add 20mL ethyl acetate and 4mL hexane, the organic layer that each the 20mL washing of water and saturated aqueous common salt is obtained is used the 3g dried over sodium sulfate, under reduced pressure boils off solvent.
By reaction product (1.19g) that silica gel chromatography obtained (ethyl acetate: hexane=1: 20), obtain 0.93g orange solids compound (c ') (yield 84%).
The rerum naturas such as NMR spectrum that below show compound (c ').
Compound (c '): 60～63 ℃ of mp
1H-NMR(400MHz，CDCl
3)：δ5.15(2H，s，PhCH
2O)，5.22-5.26(1H，m)，5.39-5.44(1H，m)，5.90(1H，d，J＝5.1Hz)，6.06(1H，ddd，J＝5.1，10.5，15.6Hz)，6.94(1H，dd，J＝2.9，9.0Hz)，7.34(1H，d，J＝2.9Hz)，7.35-7.44(5H，m)，8.04(1H，d，J＝9.0Hz)
IR(KBr)：3298，1614，1582，1506，1292，1229cm
-1
EI-MS：m/z 285(M
+)。
(3) compound (d ') is synthetic
2.00g (7.02mmol) compound (c ') is dissolved in the 20mL chloroform, under argon atmosphere, under 25 ℃ condition, adds 8.00g (4 times of amounts, 92.0mmol, 13 equivalents) Manganse Dioxide while stirring, and vigorous stirring.After 15 hours, after the disappearance of affirmation raw material, carry out suction filtration.With the solid matter that 20mL chloroform washing is obtained, merging filtrate and washings under reduced pressure boil off solvent, by silica gel chromatography residue (ethyl acetate: hexane=1: 20), obtain 1.88g white solid compound (d ') (yield 95%).
The rerum naturas such as NMR spectrum that below show compound (d ').
Compound (d '): 84～85 ℃ of mp
1H-NMR(400MHz，CDCl
3)：δ5.17(2H，s，PhCH
2O)，5.83(1H，d，J＝17.7Hz)，6.01(1H，d，J＝10.6Hz)，6.62(1H，dd，J＝10.6，17.7Hz)，6.91(1H，d，J＝2.7Hz)，7.10(1H，dd，J＝2.7，9.0Hz)，7.37-7.43(5H，m)，8.17(1H，d，J＝9.0Hz)
IR(KBr)：1686，1578，1506，1342，1244cm
-1
EI-MS：m/z 283(M
+)。
(4) compound (e) is synthetic
1.84g (6.50mmol) compound (d ') is dissolved in the 37mL ethyl acetate, adds 0.69g (0.65mmol, 10mol%) 10% palladium-charcoal on the following ice-cold stirring of argon atmosphere limit.With mixture under atmosphere of hydrogen in 25 ℃ of vigorous stirring.After 13 hours, from the reaction solution that is obtained, remove by filter catalyzer, boil off filtrate, obtain 0.87g (purity 91.14% that yield 81%, HPLC record) orange solids crude product.
By the reaction product (ethyl acetate: hexane=1: 10 → 1: 4), obtain 421mg yellow solid compound (e) (purity 95.59% that yield 84%, HPLC record) that silica gel chromatography obtained.
The rerum naturas such as NMR spectrum that below show compound (e).
131～140 ℃ of compound (e): mp
1H-NMR(400MHz，CDCl
3)：δ1.20(3H，t，J＝7.2Hz)，2.93(2H，q，J＝7.2Hz)，6.59(1H，d，J＝8.8Hz)，6.88(1H，dd，J＝2.9，8.8Hz)，7.23(1H，d，J＝2.9Hz)
IR(KBr)：3379，3296，1670，1447，1194cm
-1
EI-MS：m/z 165(M
+)。
[embodiment 8] are without the synthesis method of the compound (e) of protecting group R
(1) from compound (a) synthetic compound (c ")
500mg (2.99mmol) compound (a) is dissolved among the 15mL THF, under argon atmosphere, drips 7.5mL (7.5mmol, 2.5 equivalents) vinyl bromination magnesium THF solution (1.0M) in the about 5 minutes ice-cold stirring of inner edge limits.Stir after 1 hour, ice-cold stirring limit, limit adds reaction mixture in the 30mL 1mol/L hydrochloric acid.Then add 30mL ethyl acetate and 5mL hexane, the organic layer that each the 50mL washing of water and saturated aqueous common salt is obtained is used the 3g dried over sodium sulfate, under reduced pressure boils off solvent, obtains reaction product.
By reaction product that silica gel chromatography obtained (ethyl acetate: hexane=1: 10 → 1: 3), obtain the 541mg Huang to dark brown solid chemical compound (c ") (yield 93%).
Compound (c "):
1H-NMR(400MHz，CDCl
3)：δ5.22-5.26(1H，m)，5.35-5.40(1H，m)，5.90-5.92(1H，m)，6.06(1H，ddd，J＝5.2，10.5，15.6Hz)，6.83(1H，dd，J＝2.7，9.0Hz)，7.19(1H，d，J＝2.7Hz)，8.00(1H，d，J＝9.0Hz)。
(2) from compound (C ") synthetic compound (d ")
1.00g (5.13mmol) compound (c ") is dissolved in the 8mL acetone, and ice-cold stirring limit, limit adds 3.0mL (5mmol, 1.5 equivalents) Jones reagent.Stir after 0.5 hour, in reaction mixture, add the three ice cube sheets and the saturated sodium pyrosulfate aqueous solution of 5mL.Then add 50mL ethyl acetate and 5mL hexane, the organic layer that each the 50mL washing of water and saturated aqueous common salt is obtained is used the 5g dried over sodium sulfate, under reduced pressure boils off solvent, and acquisition 0.82g compound (d ") (yield 83%).
Compound (d "):
1H-NMR(400MHz，DMSO-d
6)：δ5.84(1H，d，J＝17.6Hz)，6.11(1H，d，J＝10.7Hz)，6.60(1H，dd，J＝10.7，17.7Hz)，6.75(1H，d，2.7Hz)，7.03(1H，dd，9.1Hz)，8.13(1H，d，J＝9.1Hz)，11.41(1H，s)。
(3) from compound (d ") synthetic compound (e)
100mg (0.513mmol) compound (d ") is dissolved in the 1mL ethyl acetate, add on the following ice-cold stirring of argon atmosphere limit 55mg (0.0513mmol, 10mol%) 10% palladium-charcoal, under atmosphere of hydrogen in stirring at room.Stir after 18 hours, remove by filter 10% palladium-charcoal, decompression boils off the solvent in the filtrate down.
Obtain 64mg yellow solid compound (e) (yield 76%).
Synthesizing of [embodiment 9] 7-ethyl-10-hydroxycamptothecines (SN-38)
With the compound (e) that obtains among the embodiment 7 (0.36g, 2.14mmol) and compound (h) (0.50g 1.82mmol) is suspended in acetic acid-toluene mixture liquid (AcOH-toluene; 1: 1,10mL) in, at room temperature add p-toluenesulphonic acids monohydrate (p-TsOHH
2O; 10mg), under nitrogen, stirred 18 hours in 100 ℃.Reaction solution is under reduced pressure concentrated, in residue, add toluene (10mL), under reduced pressure concentrate then.In residue, add acetone (9mL), stir after 2 hours, filter precipitate, the material that obtains with acetone (2mL * 2) washing and filtering.Drying under reduced pressure obtains brown solid SN-38 (purity 97.7% that 0.63g, HPLC record, yield 89%).
The HPLC operational condition:
Post: Inertsil ODS-2,4.6cm ID * 25cm (GL Science production)
Near temperature: the constant temperature 40 ℃
Flow velocity: 1mL/ minute
Moving phase: methyl alcohol: acetonitrile: the 10mM potassium primary phosphate mixes liquid (1: 1: 3)
Detect wavelength: 254nm
SN-38：
1H-NMR(400MHz，CDCl
3)：δ0.98(3H，t，J＝7Hz，CH
3)，1.38(3H，t，J＝7Hz，CH
3)，1.90(2H，q，J＝7Hz，CH
2)，3.08(2H，q，J＝7Hz，CH
2)，5.17(2H，s，CH
2O)，5.23(1H，d，J＝16Hz)，5.54(1H，d，J＝16Hz)，6.83(1H，d，J＝9Hz)，7.34-7.39(3H，m)。
[embodiment 10] 7-ethyl-10-[4-(1-piperidino-(1-position only))-1-piperidino-(1-position only)] carbonyl oxygen base camptothecine (SN-38B-11) synthetic
According to existing method (Sawada, S.; Okajima, S.; Aiyama, R.; Nokata, K.; Furuta, T.; Yokokura, T.; Sugino, E.; Yamaguchi, K.; Miyasaka, T.Chem.Pharm.Bull.1991,39,1446) (0.91g 2.32mmol) obtains SN-38B-11 (1.22g, 2.08mmol, yield 89%, optical purity 99.8%ee) from embodiment 9 synthetic SN-38.
Chirality HPLC operational condition
Post: DAICEL CHIRALCEL OD-H, 0.46cm ID * 25cm (#ODHOCE-AK031)
Guard column (Guard cartridge): DAICEL CHIRALCEL OD-H, 0.4cm ID * 1cm
Sample size: 10 μ g/10 μ L
Near temperature: the constant temperature 40 ℃
Flow velocity: 1mL/ minute
Moving phase: dimethylamine: normal hexane: alcohol mixeding liquid (1: 250: 250)
Detect wavelength: 254nm.
[embodiment 11] 7-ethyl-10-[4-(1-piperidino-(1-position only))-1-piperidino-(1-position only)] carbonyl oxygen base camptothecine hydrochloride (CPT-11) synthetic
(1.00g, 1.7mmol) (20mL 2.0mmol), dissolves by heating near 80 ℃ the hydrochloric acid of middle adding 1/10N the SN-38B-11 that obtains to embodiment 10, adds acetonitrile (100mL), at room temperature stirs and spends the night.Get precipitate, drying, moisture absorption obtains yellow-white powder CPT-11 (0.95mg, yield 89.8%).
Synthetic (1) of [embodiment 12] compounds (l)
By use n-BuLi (n-Butyl Lithium) and N-formyl radical-N near-30 ℃ or-20 ℃, N ', N '-trimethylammonium quadrol then carry out compound (k) formylation iodate and obtain compound (l) with n-BuLi and iodine near-30 ℃ or-20 ℃.
In reaction vessel, feed nitrogen, with compound (k) (5.0g; 0.028mol) be dissolved in the exsiccant tetrahydrofuran (THF) (about 66mL) near cooling-30 ℃ or-20 ℃.In the solution that is obtained, drip n-BuLi (1.6mol, hexane solution; 21.2mL, 0.034mol, 1.2 equivalents), under cooling, stir.Then add formylation reagent N-formyl radical-N, N ', N '-trimethylammonium quadrol (4.4g, 0.0034mol, 1.2 equivalents) stirs the mixture under cooling.
In the mixture that is obtained, drip n-BuLi (1.6mol, hexane solution; 35mL, 0.05mol, 2 equivalents), and under the temperature described in the table 6, stir.Then drip dry tetrahydrofuran (THF) solution (19mL) of iodine (18.4g), stir the mixture.
In the mixture that is obtained, drop into aqueous solution of sodium bisulfite (12g/200mL), after the stirring, reclaim organic layer (normal hexane), measure by the HPLC method.The result is as shown in table 6.
The HPLC operational condition
Post: Capcell Pack ODS UG120,4.6mm ID * 150mm
Moving phase: 50mM KH
2PO
4-MeCN (9: 11)
Detect wavelength: 220nm
Flow velocity: about 1mL/ minute
Temperature: room temperature
[table 6]
| Formylation (℃) Annotate 1) | Reaction times (hour) | Iodate (℃) Annotate 1) | Reaction times (hour) | Compound (k) Annotate 3) | Compound (l) Annotate 3) | Yield (%) Annotate 4) | |
| Preparation example 15 | -48～-30 -32～-29 | 3.0 | Near-70～-65-75 | 0.3 | NT Annotate 5) | 67.8 | 70.6 |
| Preparation example 16 | Near-35～-28-35 | 1.0 | -30～-20 -35～-25 | 0.5 | 5.9 | 67.8 | 71.9 |
| Preparation example 17 | -20～-15 -20～-15 | 2.0 | -10～-5 -10～-5 | 0.5 | 6.2 | 70.5 | 66.7 |
| Preparation example 18 | -10～-5 -10～-5 | 3.0 | -10～0 -10～0 | 0.5 | 3.4 | 77.6 | 63.7 |
Annotate 1) above delegation be the measured value of the internal temperature when dripping, below delegation be the temperature range of the internal temperature when stirring
Annotate 2) preparation example 15: the result of the Production Example under the report condition
Annotate 3) peak area (%)
Notes 4) yield is the percentile scaled value of peak area (%) by the HPLC method
Annotate 5) NT: undetermined
As shown in table 6, use n-BuLi as lithiation reagent, obtain the yield more than 60% or 60%.Shown in preparation example 16, under-40 ℃～-30 ℃ constant temperature, can react with the good yield more than 70% or 70%.
The purifying of [embodiment 13] compounds (l) (dilute hydrochloric acid washing)
(5.0g 0.028mol) is dissolved in the dry tetrahydrofuran (about 66mL), under near the constant temperature-35 ℃, reacts similarly to Example 12 with compound (k).Reclaim the reaction mixture (normal hexane layer) obtained, use the dilute hydrochloric acid with organic layer equivalent to wash.
After the washing, behind anhydrous magnesium sulfate drying, filter organic layer, the part of filtrate under the condition identical with embodiment 12, is measured by the HPLC method.The result is as shown in table 7.
[table 7] washs the result of the organic layer behind each hexane layer with dilute hydrochloric acid
| Hexane layer Annotate 1)(mL) | Dilute hydrochloric acid (mol/L) | Residue amount (g) | Compound (k) Annotate 2) | MTPC Annotate 2,3) | Compound (l) Annotate 2) | The rate of recovery (%) |
| 25 | Annotate 4) - | 1.5 | 6.0 | 11.8 | 54.7 | - |
| 50 | 0.1 | 2.9 | 6.6 | 12.4 | 58.7 | 100 |
| 50 | 1.0 | 2.6 | 1.8 | 13.0 | 61.2 | 100 |
| 50 | 2.5 | 2.6 | 0.4 | 12.6 | 62.4 | 100 |
| 50 | 3.5 | 2.6 | 0.2 | 12.7 | 64.2 | 100 |
Annotate 1) will be divided into 5 parts (25mL non-processor, 4 50mL wash) from the hexane layer that reaction mixture obtains.
Annotate 2) peak area %
Annotate 3) MTPC:2-methoxyl group-6-trimethyl silyl pyridine-3-formaldehyde
Annotate 4) not washing
As shown in table 7, by the dilute hydrochloric acid washing, compound (k) almost all is removed.Especially, by with 1.0mol/L and the washing of the dilute hydrochloric acid more than the 1.0mol/L, can obtain highly purified compound (l).On the other hand, reaction intermediate 2-methoxyl group-6-trimethyl silyl pyridine-3-formaldehyde (MTPC) then almost is not removed.MTPC is the formylation intermediate of compound (k).
The purifying (dilute hydrochloric acid progressively washs) of [embodiment 14] compounds (l)
(5.0g 0.028mol) is dissolved in the dry tetrahydrofuran (about 66mL), under near the constant temperature-35 ℃, reacts similarly to Example 12 with compound (k).Reclaim the reaction mixture (normal hexane layer) obtained, use the dilute hydrochloric acid with the different concns as shown in table 8 of organic layer equivalent progressively to wash.
After the washing, separate the tart water layer,, then use n-hexane extraction with the yellow soda ash neutralization.Behind the anhydrous magnesium sulfate drying organic layer, filter, under the condition identical, measure the part of filtrate by the HPLC method with embodiment 12.The result is as shown in table 8.
The order of [table 8] according to the form below (on → down) respectively with the hexane layer after the dilute hydrochloric acid washing aftertreatment, its water layer that neutralizes is with the result of hexane extraction organic layer
| Dilute hydrochloric acid (mol/L) | Residue (g) Annotate 1) | Compound (k) Annotate 2) | Compound (l) Annotate 2) | The rate of recovery (%) |
| Washing | - | NT Annotate 3) | NT | - |
| 0.1 | 0.40 | 21.9 | 10.7 | - |
| 0.1 | 0.04 | NT | NT | - |
| 1.0 | 0.21 | 67.0 | 13.7 | - |
| 2.5 | 0.28 | 71.0 | 3.0 | - |
| 5.0 | 0.54 | 18.0 | 4.0 | - |
| Residue after the washing Annotate 4) | 7.27 | ND Annotate 5) | 77.9 | 98.3 |
Notes 1) washing of each dilute hydrochloric acid neutralizes the mixture n-hexane extraction with yellow soda ash.Dry organic layer filters, and drying under reduced pressure filtrate is to doing then
Annotate 2) HPLC (peak area %)
Annotate 3) undetermined
Annotate 4) residue of the hexane layer after the washing progressively
Notes 5) do not reach detectability
As shown in table 8, progressively wash by dilute hydrochloric acid with different concns, obtained highly purified compound (l).
The purifying (passing through distillation purifying) of [embodiment 15] compounds (l)
(5.0g 0.028mol) is dissolved in the dry tetrahydrofuran (about 66mL), under near the constant temperature-35 ℃, reacts similarly to Example 12 with compound (k).Reclaim the reaction mixture (normal hexane layer) obtained, under reduced pressure distill under (near decompression degree: the 0.35mmHg), 81～99 ℃ the temperature.After the distillation, use silicagel column, then with normal hexane-ethyl acetate mixed solution (50: 1) purifying residue, obtain purified product with normal hexane.
The residue and the purified product that are obtained are measured by the HPLC method under the following conditions.The result is as shown in table 9.
The HPLC operational condition
Post: Capcell Pack ODS UG120,4.6mm ID * 150mm
Moving phase: 50mM KH
2PO
4-MeCN mixed solution (1: 1)
Detect wavelength: 220nm
Flow velocity: about 1mL/ minute
Temperature: room temperature
Result during the concentrating residues thing of the hexane layer of [table 9] distillation after the aftertreatment
| Cut (g) | Compound (k) Annotate 1) | MTPC Annotate 1) | Compound (l) Annotate 1) | The rate of recovery (%) | |
| Thing before the distillation | - | 3.6 | 13.5 | 71.5 | - |
| Partly-1 | 0.19 | 47.3 | 36.1 | 8.7 | - |
| Partly-2 | 1.16 | 8.9 | 53.7 | 28.8 | - |
| Trap | 1.17 | 70.3 | ND Annotate 2) | ND | - |
| The still residue | 5.13 | 0.3 | 3.1 | 89.9 | 75.9 |
| The column purification product Post 3) | - | - | 3.9 | 95 |
Annotate 1) peak area %
Annotate 2) do not detect: below detectability
Annotate 3) by silica gel chromatography final residue thing
As shown in table 9, almost removed whole MTPC by distillation.In addition, by silica gel chromatography, obtained the compound (l) of extreme high purity.On the other hand, owing to observe the painted of compound (l) and decompose being higher than under this temperature distillation, thereby not preferred.
The purifying of [embodiment 16] compounds (l) (with the recovery of hydrochloride calculating)
With compound (k) (5.0g 0.028mol) is dissolved in dry tetrahydrofuran (about 66mL).Under near the constant temperature-35 ℃, react similarly to Example 12.The reaction mixture that 10g obtained is dissolved in the hydrochloric acid (10mL) of 10N, and at room temperature stirs.After the stirring, filter and to obtain the xanchromatic precipitate, and with a spot of 10N salt acid elution.After the washing, it is dissolved in the water (about 10mL), adds sodium bicarbonate pH is reached near about 8, use n-hexane extraction, and vapourisation under reduced pressure is to doing.
The residue that obtained under the condition identical with embodiment 15, is measured by the HPLC method.The result is as shown in table 10.
The result that [table 10] neutralizes and extract the hydrochloride in 10mol/L hydrochloric acid
| (g) Annotate 1) | Compound (k) Annotate 2) | MTP annotates 2) | Compound (l) Annotate 2) | The rate of recovery (%) | |
| Before the processing | 10 | - Annotate 3) | 16.9 | 61.8 | - |
| After the processing | 6 | - | 3.3 | 90.0 | 87.4 |
Annotate 1) the residue amount
Annotate 2) peak area %
Annotate 3) undetermined
As shown in table 10, become the purification process of hydrochloride by making reactant, MTPC almost all is removed.
Compound (l): yellow oily material
1H-NMR(400MHz，CDCl
3)：δ0.30(9H，s)，4.05(3H，s)，7.67(1H，s)，10.19(1H，s)
EI-MS：m/z 335(M
+)。
Synthesizing of [embodiment 17] compounds (m)
In the formula, TMS represents trimethyl silyl, and Me represents methyl.
Will be under nitrogen atmosphere, in 0～5 ℃ to compound (l) (20.00g, 56.0mmol, content 93.9%), triethyl-silicane (17.9mL, 112.0mmol, 2 equivalents) and in the mixed solution of crotyl alcohol (15.7mL, 184.8mmol, 3.3 equivalents) drip trifluoroacetic acid (28.5mL, 375.3mmol, 6.7 equivalent), stirred 30 minutes, afterwards stir about 20 hours under room temperature.In reaction mixture, inject aqueous sodium carbonate (20.8g is dissolved in 277mL water) and normal hexane (56mL), separate organic layer, with normal hexane (56mL) aqueous layer extracted.Merge organic layer and under reduced pressure be concentrated into dried.By silica gel column chromatography [silica gel: Fuji Silysia PSQ100B (about 4 times of weights of compound (1)), elutriant: n-hexane/ethyl acetate (73: 3)] residue is carried out purifying, obtain table 11 (preparation example 20).Table 11 (preparation example 19) is method (Josien, the H. according to H.Josein etc.; Ko, S.B.; Bom, D.; Curran, D.P., Chem.Eur.J.1998,4,67-83, Curran, D.P.; Ko, S.B.; Josein, H., Angew.Chem.Int.Ed.Engl.1995,34,2683-2684) prepare.Under the condition of report, use methylene dichloride as reaction solvent.Without methylene dichloride the time, obtain the quality product (m) identical with yield.
[table 11]
The HPLC operational condition
Post: Intertsil ODS-2,5 μ m, 4.6mm I.D. * 250mm (GL Science production)
Near temperature: the constant temperature 40 ℃
Moving phase: acetonitrile/0.01mol/l potassium primary phosphate (5: 1)
Flow velocity: 1mL/ minute
Detect wavelength: 254nm.
Synthetic (2) of [embodiment 18] compounds (l)
In the formula, TMS represents trimethyl silyl, and Me represents methyl.
(v) (1.00g, purity 98.43% add TEMPO (2.3mg, 0.015mmol, 0.005 equivalent) and 7% (w/v) NaHCO in toluene 2.9mmol) (8.7mL) solution to compound
3(6.98mL), after cooling under 0～5 ℃, add aqueous sodium hypochlorite solution (available chlorine: minimum 5%, 4.5g, 3.0mmol, 1.05 equivalents), stirring is 2 hours under 0～5 ℃.In reaction solution, add 10%Na
2SO
3(3.7mL, 2.9mmol), stirring is after 30 minutes down at 0～5 ℃, and the elimination insolubles is with toluene (1mL * 3) washing.With the organic layer separatory, anhydrous Na is used in water (10mL) washing
2SO
4(2g) drying is filtered, and then uses toluene wash, under reduced pressure is concentrated into driedly, obtains 0.93g (yield 87%) yellow oily compound (l), content 90.60% (recording (reference example 17) by HPLC).
Synthetic (1) of [embodiment 19] compounds (n)
In the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl.
Compound (m) (1.60g) is dissolved in the solvent shown in about 30mL table 12, adds 0.83g Tetrabutyl amonium bromide (Bu
4NBr), 0.71g salt of wormwood (K
2CO
3) and 57mg palladium (Pd (OAc)
2), react under the conditions shown in Table 12.To react mixed liquid placement and be cooled to room temperature, the normal hexane under injection 18mL is ice-cold.
By Celite pad elimination insolubles, with 6mL normal hexane washing 3 times.With 9mL water washing filtrate 2 times, use anhydrous sodium sulfate drying, and under reduced pressure concentrate.By silica gel column chromatography, as elutriant purifying residue, obtain preparation example 21～27 with the mixed liquid (95: 5) of ethyl acetate hexane.
The preparation example 21 of table 12 is method (Josien, the H. according to H.Hosien etc.; Ko, S.B.; Bom, D.; Curran, D.P., Chem.Eur.J.1998,4,67-83., Curran, D.P.; Ko, S.B.; Josien, H., Angew.Chem.Int.Ed.Engl.1995,34,2683-2684) prepare.Measure the interior type of the preparation example 21～27 that is obtained and the amount of external form by HPLC.
[table 12]
DMF:N, dinethylformamide, MeCN: acetonitrile, THF: tetrahydrofuran (THF), than: the peak area of the peak area/external form of interior type (HPLC),
-: yield is not calculated
As shown in table 12, carry out boiling reflux by using THF as reaction solvent, the ratio of interior type and external form is about 7, and selectivity is improved (preparation example 25～27), and yield has also improved more than 10% or 10% than preparation example.
HPLC operational condition: reference example 17
Synthetic (2) of [embodiment 20] compounds (n)
With compound (m) (1.27g, 2.6mmol, content 78.7%) be dissolved in diisopropyl ether-acetonitrile-water mixed solution (4: 3: 1,20mL) in, under room temperature, add Tetrabutyl amonium bromide (0.82g, 2.6mmol), N, N-diisopropylethylamine (3.48mL, 20.8mmol, 8 equivalents) and palladium (57mg, 0.26mmol), boiling reflux 30 minutes.After being cooled to reaction mixture below 20 ℃ or 20 ℃, filter, and wash with normal hexane (2.6mL * 3).In filtrate, add normal hexane (10mL) and 10%Na
2SO
3(16mL, 13.0mmol, 5 equivalents) are with the organic layer separatory.With 1N HCl (16.4mL), then water (10mL * 2) washs organic layer.Under reduced pressure organic layer is concentrated into driedly, obtains brown oily compound (n) (0.83g, the content 73.34% that 2.325mmol, HPLC record, yield 91%, interior type/external form ratio: 10.6).
Compare with preparation example 21, its selectivity improves greatly, and yield has also improved 20%.
Synthesizing of [embodiment 21] compounds (o)
In the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl.
To six cyano group iron (III) (195.7g, 0.59mol), salt of wormwood (82.1g, 0.59mol) and amsacrine (37.7g adds in the aqueous solution water (990mL) 0.40mol) (DHQD)
2PYR (4.36g, 4.95mmol) and two hydration potassium osmates (VI) (1.0mmol), near 5 ℃, stirred 1 hour.In this solution, add compound (n) (77.8g, 0.18mol, content 61.5%), then near 5 ℃, stirred 20 hours.The S-WAT (74.9g) that adds solid state in reaction solution stirs after 30 minutes, by Celite pad elimination insolubles near these 5 ℃.Wash insolubles (4 times, be total to 770mL) with ethyl acetate.The organic layer of separating filtrate is then with ethyl acetate (770mL) aqueous layer extracted.With the organic layer anhydrous sodium sulfate drying that merges, filter, then under reduced pressure concentrate.By silica gel column chromatography [silica gel: Fuji Silysia PSQ100B (compound (n) about 3.5 times), elutriant: dichloromethane/ethyl acetate mixed solution (4: 1)] purifying residue, obtain russet solid chemical compound (o).
The result's (preparation example 29) who obtains the situation of compound (p) is presented in the table 13.
As shown in table 13, use than the low potassium osmate of perosmic anhydride (VIII) volatility also to obtain in equal result aspect yield and the optical purity.
[table 13]
| Oxygenant | Yield (%) | Optical purity (%ee) | |
| Preparation example 28 | OsO 4 | 82～95 | 95.6～96.2 |
| Preparation example 29 | K 2OsO 4·2H 2O | 94 | 95.9 |
Preparation example 28 is according to method (Josein, the H. of H.Josien etc.; Ko, S.B.; Bom, D.; Curran, D.P., Chem.Eur.J.1998,4,67-83., Curran, D.P.; Ko, S.B.; Josein, H., Angew.Chem.Int.Ed.Engl.1995,34,2683-2684) preparation.
%ee: the compound (o) that is obtained is changed into compound (p) by the method for putting down in writing among the embodiment 22, and its optical purity is measured by chirality HPLC method (reference example 22).
Synthesizing of [embodiment 22] compounds (p)
In the formula, TMS represents trimethyl silyl, and Me represents methyl, and Et represents ethyl.
70g compound (o) is dissolved in the 1L methanol aqueous solution (10: 1), at room temperature adds the iodine of the amount of Table 12 and the lime carbonate of 47.1g solid state, react under the conditions shown in Table 14.
The reaction mixture placement that is obtained is cooled to room temperature, adds 1L 10% S-WAT and 1L chloroform, at room temperature stirred 30 minutes, remove by filter insolubles.Separating filtrate is with 500mL chloroform extraction water layer.Organic layer behind the separating, washing is further with 500mL chloroform washing water layer.Merge organic layer and use anhydrous acid sodium drying, filter, then under reduced pressure concentrate.
Similarly to Example 19, calculate the yield of the compound that is obtained, the result is as shown in table 14.
As shown in table 14, by with 4 normal iodine boiling refluxs, be reflected in reaction times of 1/9～1/10 of preparation example 30 and finish, and show equal yield.
[table 14]
| Iodine (equivalent) | Temperature | Reaction times (hour) | Yield (%) | |
| Preparation example 30 | 9 | Room temperature | 48 | 86 |
| Preparation example 31 | 4 | Room temperature | 72 | 86 |
| Preparation example 32 | 4 | 40℃ | 48 | 88 |
| Preparation example 33 | 4 | 60℃ | 20 | 88 |
| Preparation example 34 | 4 | Boiling reflux | 5 | 84 |
Preparation example 30 is according to method (Josien, the H. of H.Josien etc.; Ko, S.B.; Bom, D.; Curran, D.P., Chem.Eur.J.1998,4,67-83, Curran, D.P.; Ko, S.B.; Josien, H., Angew.Chem.Int.Ed.Engl., 1995,34,2683-2684) preparation.
The HPLC operational condition:
Post: GL Science Inertsil ODS-2 (0.46cm φ * 25cm)
Sample size: 2 μ g/10 μ l
Temperature: 40 ℃
Flow velocity: 1mL/ minute
Moving phase: acetonitrile: 10mM potassium primary phosphate (5: 3)
Detect wavelength: 254nm
Chirality HPLC operational condition:
Post: DAICEL CHIRALCEL OD-H (#ODH0CE-AK031,0.46cm φ * 25cm)
Guard column: DAICEL CHIRALCEL OD-H (0.4cm φ * 1cm)
Sample size: 10 μ g/10 μ l
Near temperature: the constant temperature the room temperature
Flow velocity: 0.5mL/ minute
Moving phase: normal hexane: alcohol mixeding liquid (200: 1)
Detect wavelength: 254nm.
Synthesizing of [embodiment 23] compounds (q)
To solvent (about 400mL; Record in the table 15) add the reagent in the table in the solution of compound (p) in, with the mixture that obtained in table time and temperature under stir.In reaction mixture, add 1.7L 20% yellow soda ash, 1.0L 10% S-WAT and 550mL chloroform, and stir.Separate organic layer, use 550mL chloroform extraction 2 times.The combined chloroform layer is used anhydrous sodium sulfate drying, filters, and carries out HPLC mensuration with being evaporated to the compound (q) of doing and obtaining.The result is as shown in Table 15.
By in acetic acid, fully carrying out this conversion (preparation example 39) with NCS-NaI at 65 ℃.Reaction is finished required time and can significantly be shortened, more than the comparative example 1 high 50% or 50% of yield than existing report of compound (q) under this condition.
[table 15]
| Reaction solvent | Reagent | Equivalent | Temperature | Reaction times (hour) | Yield (%) | |
| Preparation example 35 | AcOH | NIS | 12 | 65℃ | 45.0 | 63 |
| Preparation example 36 | CH 2Cl 2 | I 2-CF 3CO 2Ag | 2 | Room temperature | 1716.5 | 97 |
| Preparation example 37 | AcOH | NCS-NaI | 6 | 65℃ | 16.0 | 95 |
| Preparation example 38 | AcOH | NCS-NaI | 6 | 65℃ | 15.0 | 93 |
| Preparation example 39 | AcOH | NCS-NaI | 6 | 65℃ | 15.0 | 94 |
| Comparative example 1 | X) | ICI | 4 | 0 ℃～room temperature | 48.0 | 45 |
X) methylene dichloride (CH
2Cl
2): the mixed solvent of chloroform=3: 2,
ICI: iodine monochloride, NIS:N-iodo succinimide, NCS:N-chlorosuccinimide, Eq: the mol ratio of agents useful for same, yield: separation yield.
The purification process I of [embodiment 24] compounds (q)
The reaction mixture that contains compound (q) (purity 89.2% that HPLC records) that obtains among the 63g embodiment 23 is suspended in the 150mL methyl alcohol, under agitation drips the aqueous sodium hydroxide solution of 0.2N and continue stirring 2 hours.With basic solution washing 3 times, the hydrochloric acid of using 6N is used 400mL chloroform extraction 3 times with pH regulator to 1～2 with the 400mL chloroform.With the chloroform layer that is obtained anhydrous sodium sulfate drying, dried after the filtration by under reduced pressure being concentrated into, obtain compound (q) (purity 97.7% (peak area; HPLC operational condition: reference example 25)) (preparation example 40).
The purification process II of [embodiment 25] compounds (q)
The purified product that 50g is contained the compound (q) that obtains among the embodiment 24 is dissolved in the 240mL chloroform, carries out lamination with the 400mL normal hexane, at room temperature leaves standstill 15 hours.The crystallization that filtration is separated out, filtrate under reduced pressure are concentrated into dried, obtain compound (q) (preparation example 41).
By present method the compound (q) (preparation example 40, optical purity 93～96%) that obtains among the embodiment 24 is carried out the optics purifying.The optical purity that the compound that is obtained (q) (preparation example 41) records by following chirality HPLC method is 99.7～99.9%.
The HPLC operational condition:
Post: Inertsil ODS-2,0.46cm I.D. * 25cm (GL Science production)
Near temperature: the constant temperature 40 ℃
Flow velocity: 1mL/ minute
Moving phase: (5: 3) of acetonitrile/10mM potassium primary phosphate
Detect wavelength: 254nm
Chirality HPLC operational condition:
Post: DAICEL CHIRALPAK AD-H (#ADH0CE-BC037,0.46cmID * 25cm)
Guard column: DAICEL CHIRALPAK AD-H (0.4cm ID * 1cm)
Near temperature: the constant temperature about 25 ℃
Flow velocity: 1mL/ minute
Moving phase: normal hexane: IPA mixed solution (25: 1)
Detect wavelength: 254nm.
The preparation method of [embodiment 26] compounds (r)
At room temperature, to compound (q) (42.8g, 0.10mol, content 84.5%) 1-propyl alcohol (490mL) solution in add palladium (1.34g, 6.0mmol) and salt of wormwood (24.7g, 0.18mol), outgas by in reaction vessel, reducing pressure, replace, again by the decompression degassing with nitrogen, with the carbon monoxide displacement, stirred 18 hours down at 60 ℃.Be cooled to room temperature, by Celite pad elimination insolubles, with ethyl acetate (300mL) washing.In filtrate, add 1N hydrochloric acid (150mL) and saturated aqueous common salt (300mL), separate organic layer, add ethyl acetate (300mL) and extract.Merge organic layer, use anhydrous sodium sulfate drying, filter, then under reduced pressure be concentrated into dried.By silica gel column chromatography [silica gel: Fuji Silysia PSQ100B (about 3.5 times of weights of compound (r), elutriant: purifying residue chloroform/methanol mixed solution (99: 1)), obtain brown oil compound (r) (content 73.4% that 30.3g, HPLC record, yield 70%).
The HPLC operational condition:
Post: GL Science Inertsil ODS-2 (0.46cm ID * 25cm)
Near temperature: the constant temperature 40 ℃
Flow velocity: 1mL/ minute
Moving phase: 10mM potassium primary phosphate: acetonitrile mixed solution (4: 3)
Detect wavelength: 254nm
The preparation method of [embodiment 27] compounds (s)
Under nitrogen atmosphere under the shading, in room temperature to compound (r) (28.7g, 68.2mmol, content 73.4%) and sodium iodide (27.6g, 0.18mol) anhydrous acetonitrile (141mL) solution in add the chloro trimethyl silyl (23.3mL 0.18mmol), and stirred 3 hours.In reaction mixture, add 1N hydrochloric acid (8mL), then add 10% S-WAT (232mL), at room temperature stirred 30 minutes.With the mixed liquid of ethyl acetate extraction, with the organic layer separatory, then under reduced pressure be concentrated into driedly, obtain compound (s) (content 85.6% that 22.3g, HPLC record (reference is following), yield 95%).
The HPLC operational condition:
Post: Inertsil ODS-2,0.46cm I.D. * 25cm (GL Science production)
Near temperature: the constant temperature 40 ℃
Flow velocity: 1mL/ minute
Moving phase: 10mM potassium primary phosphate/acetonitrile (5: 2)
Detect wavelength: 254nm.
The preparation method of [embodiment 28] compounds (t)
0.50g compound (s) is dissolved in the 7mL methyl-sulphoxide (hereinafter referred to as DMSO), under room temperature, adds 0.40g cesium carbonate (Cs
2CO
3) or salt of wormwood (K
2CO
3), under argon atmospher, stirred 20 minutes in 50 ℃.Dropwise addition of acrylic acid tert-butyl ester 2.1mL (1.8g) in mixture stirred 24 hours in 50 ℃ under argon atmospher.In ice-cold stirring downhill reaction mixture, add 10mL water and 1mL concentrated hydrochloric acid.Extract mixture 4 times with 7mL toluene-ethyl acetate mixed solution (4: 1).Merge organic layer, with 5mL water washing 3 times, behind anhydrous sodium sulfate drying, filter, then vapourisation under reduced pressure obtains preparation example 42 and preparation example 43 to doing.Analyze the residue that is obtained by HPLC (with reference to following).
Shown in table 16, under with the situation of cesium carbonate as alkali, the yield of compound (t) is 72% (preparation example 42), and on the other hand, under with the cheap situation of salt of wormwood as alkali, the yield and the preparation example 42 of compound (t) are equal.
[table 16]
The HPLC operational condition:
Post: Inertsil ODS-2,0.46cm I.D. * 25cm (GL Science production)
Near temperature: the constant temperature 40 ℃
Flow velocity: 1mL/ minute
Moving phase: 10mM potassium primary phosphate/acetonitrile (5: 2)
Detect wavelength: 254nm.
[embodiment 29] SN-38's is synthetic
With compound (h) (0.50g, 1.82mmol, content 96.6%) and compound (e) (0.36g, mixture 2.18mmol) the nitrogen atmosphere low suspension toluene-acetic acid mixed solution (1: 1,10mL) in, under room temperature, add p-TsOHH
2O (10mg) stirred 7 hours in 90 ℃.Put be chilled to room temperature after, under reduced pressure concentrate.In residue, add toluene (10mL), and after under reduced pressure concentrating (this operation repeats 2 times to remove acetic acid), adding acetone (9mL), under nitrogen atmosphere in stirring at room 30 minutes.Filter insolubles, with acetone (2mL * 2) washing, then drying under reduced pressure obtains loess look solid SN-38 (purity 99.6% that 0.63g, HPLC record (reference example 9), yield 89.1%) (preparation example 45).
The preparation example 44 of table 17 is according to P﹠amp; The method of U (Henegar, K.E.; Ashford, S.W.; Baughman, T.A.; Sih, J.C.; Gu, R.L., J.Org.Chem.1997,62,6588-6597) preparation.
Shown in table 17, by reacting under atmosphere of inert gases, yield and purity are improved.
[table 17]
| Purity (%) | Yield (%) | ||
| Preparation example 44 | In the air | 97.6 | 75 |
| Preparation example 45 | Nitrogen | 99.6 | 89 |
[embodiment 30] tricycle kentones complete synthesis
The preparation process that below shows tricycle kentones (compound (h)).
(1) compound (m) is synthetic
Under nitrogen atmosphere, in 0～5 ℃ to compound (l) (20.0g, 56.0mmol, 2 equivalents, content 93.9%), triethyl-silicane (17.9mL, 112mmol, 2 equivalents) and crotyl alcohol (15.7mL, 184.8mmol, 3.3 after dripping trifluoroacetic acid (28.5mL, 375.2mmol, 6.7 equivalents) in mixed solution equivalent), stirred 30 minutes, afterwards stir about 20 hours under room temperature.In reaction mixture, inject aqueous sodium carbonate (20.8g is dissolved in 277mL water) and normal hexane (56mL), separate organic layer, with normal hexane (57mL) aqueous layer extracted.The organic layer that extraction merges.Under reduced pressure be concentrated into the organic layer that merges dried.By silica gel column chromatography [silica gel: Fuji Silysia PSQ100B (80g), elutriant: n-hexane/ethyl acetate (73: 3)] the purifying residue, remove by-product compounds (v) (4.95g from two, 14.68mmol, purity 98.43%, yield 26%), obtain yellow oily compound (m) (17.8g, the content 80.0% that HPLC records, yield 64%).
Below show HPLC condition that assay is used and compound (m), compound (NMR spectrum v).
HPLC operational condition: reference example 17
Compound (m):
1H-NMR (400MHz, CDCl
3): δ 0.24 (9H, s, TMS), 1.69 (3H, dd, J=1.0,6.1Hz ,=CHC
H 3 ), 3.85-4.05 (2H, m, OC
H 2 CH=), 3.93 (3H, s, CH
3O), 4.55 (2H, s, OCH
2), 5.55-5.83 (2H, m, CH=CH), 7.47 (1H, s)
Compound (v):
1H-NMR (400MHz, CDCl
3): δ 0.27 (9H, s, TMS), 2.45 (1H, t, J=6.8Hz, OH), 3.99 (3H, s, CH
3O), 4.79 (2H, d, J=6.8Hz, C
H 2 OH), 7.49 (1H, s).
(2) compound (n) is synthetic
With compound (m) (1.27g, 2.555mmol, content 78.73%) be dissolved in the mixed liquid of diisopropyl ether-acetonitrile-water (4: 3: 1,20mL), in room temperature add Tetrabutyl amonium bromide (0.82g, 2.56mmol), N, N-diisopropyl ether ethamine (3.48mL, 2.555 * 8mol) and palladium (57mg, 0.26mmol), boiling reflux 30 minutes.Reaction mixture is cooled to below 20 ℃ or 20 ℃, filters, with normal hexane (10mL) washing.In filtrate, add normal hexane (10mL) and 10%Na
2SO
3(16mL, 113mmol, 5 equivalents) are with the organic layer separatory.With 1N HCl (16.4mL), then water (10mL * 2) washs organic layer.Under reduced pressure organic layer is concentrated into driedly, obtains brown oily compound (n) (0.83g, the content 73.34% that 2.325mmol, HPLC record, yield 91%, interior type/external form: 10.6.
Below show the HPLC condition that assay is used and the NMR spectrum of compound (n).
HPLC operational condition: reference example 17
Compound (n):
1H-NMR (400MHz, CDCl
3): δ 0.26 (9H, s, TMS), 1.12 (3H, t, J=7.3Hz, CH
2CH
3), 2.31 (2H, dq, J=1.0,7.3Hz, CH
2CH
3), 3.94 (3H, s, OCH
3), 5.00 (2H, s, OCH
2), 6.51 (1H, t, J=1.0Hz, OCH=), 6.83 (1H, s, aromatic ring-H).
(3) compound (o) is synthetic
To the sour potassium of six cyano group iron (III) (195.7g, 0.59mol), salt of wormwood (82.1g, 0.59mol) and amsacrine (37.7g adds in aqueous solution 0.40mol) (990mL) (DHQD)
2PYR (4.36g, 4.95mmol) and two hydration potassium osmates (VI) (0.99mmol), near 5 ℃, stirred 1 hour.In this solution, add compound (n) (77.8g, 0.18mol, content 61.5%), then near 5 ℃, stirred 20 hours.
In reaction solution, add solid state S-WAT (74.9g), near 5 ℃, stirred 30 minutes, by Celite pad elimination insolubles.Wash insolubles (4 times, be total to 770mL) with ethyl acetate.The organic layer of separating filtrate is then with ethyl acetate (770mL) aqueous layer extracted.Merge organic layer, use anhydrous sodium sulfate drying, filter, then under reduced pressure concentrate.By silica gel column chromatography [silica gel: Fuji Silysia PSQ100B (700g), elutriant: dichloromethane/ethyl acetate mixes liquid (4: 1)] purifying residue, obtain russet solid chemical compound (o).
(4) compound (p) is synthetic
With 70.2g compound (o) be dissolved in the mixed liquid of methanol-water (10: 1,1.0L) in, under room temperature, add solid iodine (183.7g, 0.72mol) and lime carbonate (36.23g, 0.36mol), boiling reflux 5 hours.Reaction mixture put be chilled to room temperature, add 10% S-WAT (1.0L) and chloroform (1.0L), at room temperature stirred 15 minutes, filter insolubles, the material that obtains with chloroform (0.5L) washing and filtering.
Separate organic layer, then with chloroform (0.5L) aqueous layer extracted.Merge organic layer, use anhydrous sodium sulfate drying, filter, under reduced pressure be concentrated into dried, acquisition russet oily mater compound (p) [content 80.4% that 53.6g, HPLC record, from the total recovery 81% of compound (m), chirality HPLC records 96.2%ee].
Below show the HPLC condition that assay is used and the NMR spectrum of compound (p).
HPLC operational condition: reference example 22
Chirality HPLC operational condition: reference example 22
Compound (p):
1H-NMR (400MHz, CDCl
3): δ 0.28 (9H, s, TMS), 0.94 (3H, t, J=7.4Hz, CH
2C
H 3 ), 1.76 (2H, q, J=7.4Hz, C
H 2 CH
3), 3.61 (1H, s, OH), 3.98 (3H, s, OCH
3), 5.23 (1H, d, J=15.6Hz), 5.54 (1H, d, J=15.6Hz), 7.33 (1H, s, aromatic ring-H).
(5) compound (q) is synthetic
With compound (p) (50.2g, 0.14mol, content 80.4%, 96.2%ee) be dissolved in the acetic acid (411mL), under room temperature, add the solid N-chlorosuccinimide (107.36g, 0.80mol) and sodium iodide (120.52g, 0.80mol), stirred 16 hours down in about 65 ℃.Put be chilled to room temperature after, inject 20% yellow soda ash (1.7L), 10% S-WAT (1.0L) and chloroform (0.6L) while stirring.Separate organic layer, then water layer is extracted 2 times with chloroform (0.6L).Merge organic layer, use anhydrous sodium sulfate drying, filter, then under reduced pressure be concentrated into dried (crude product (q)).
(6) the purifying I of compound (q)
Residue (compound of crude product (q), the purity 89.2% that HPLC records) is suspended in the methyl alcohol (150mL), under agitation is added drop-wise in the 0.2N sodium hydroxide (0.40mol), at room temperature continue to stir 2 hours.With this alkali aqueous solution washing 3 times, separating water layer with chloroform (400mL), is 1～2 with 6N hydrochloric acid with pH regulator, with chloroform (400mL) extraction 3 times.Separate organic layer, use anhydrous sodium sulfate drying, filter, under reduced pressure be concentrated into dried ((q) of purge process, the purity that HPLC records: 97.7%).
(7) the purifying II of compound (q)
(q) of purge process is dissolved in the chloroform (280mL), and lamination normal hexane (400mL) at room temperature left standstill 15 hours.The crystallization that filtration is separated out, filtrate under reduced pressure are concentrated into dried, and acquisition dark brown tarring compound (q) (47.4g, the content 84.5% that 0.115mol, HPLC record, yield 86%, chirality HPLC records 99.7%ee).
The NMR spectrum and the specific optical rotation that below show HPLC condition, chirality HPLC condition and compound (q) that assay is used.
HPLC operational condition: reference example 22
Chirality HPLC operational condition: reference example 25
Compound (q):
1H-NMR (400MHz, CDCl
3): δ 0.94 (3H, t, J=7.3Hz, CH
2C
H 3 ), 1.75 (2H, q, J=7.3Hz, C
H 2 CH
3), 3.58 (1H, s, OH), 3.96 (3H, s, OCH
3), 5.16 (1H, d, J=15.6Hz), 5.47 (1H, d, J=15.6Hz), 7.59 (1H, s, aromatic ring-H)
[α]
D 20＝+51.3(c＝0.981，CHCl
3)。
(8) compound (r) is synthetic
At room temperature, to compound (q) (42.8g, 0.10mol, content 84.5%) 1-propanol solution (490mL) in add palladium (1.34g, 6.0mmol) and salt of wormwood (24.67g, 0.179mol), outgas by in reaction vessel, reducing pressure, and use argon replaces, then again by the decompression degassing, with the carbon monoxide displacement, stirred 4 hours down at 60 ℃.Put cold put room temperature after, filter insolubles by the Celite pad, with ethyl acetate (300mL) washing.In filtrate, add 1N hydrochloric acid (150mL) and saturated aqueous common salt (300mL), separate organic layer, then in water layer, add ethyl acetate (300mL) and extract.Merge organic layer, use anhydrous sodium sulfate drying, filter, then under reduced pressure be concentrated into dried.By silica gel column chromatography [silica gel: 200g, elutriant: chloroform: methyl alcohol mixes liquid (99: 1)] purifying residue, obtain brown oil materialization compound (r) (30.3g, the content 73.4% that 72.9mmol, HPLC record, yield 70%).
Below show the HPLC condition that assay is used and the NMR spectrum of compound (r).
HPLC operational condition: reference example 26
Compound (r):
1H-NMR (400MHz, CDCl
3): δ 0.88 (3H, t, J=7.3Hz, CH
3), 1.04 (3H, t, J=7.3Hz, CH
3), 1.82 (4H, m, CH
2* 2), 3.69 (1H, s, OH), 4.09 (3H, s, OCH
3), 4.34 (2H, t, J=6.8Hz, CH
2), 5.31 (1H, d, J=16.3Hz), 5.61 (1H, d, J=16.3Hz), 7.94 (1H, s, aromatic ring-H).
(9) compound (s) is synthetic
Under nitrogen atmosphere under the shading, in room temperature to compound (r) (28.7g, 68.2mmol, content 73.4%) and sodium iodide (27.6g, 0.184mol) anhydrous acetonitrile (141mL) solution in add the chloro trimethyl silyl (23.3mL 0.18mmol), and stirred 3 hours.In the mixed liquid of reaction, add 1N hydrochloric acid (8mL), then add 10% S-WAT (232mL), under room temperature, stirred 30 minutes.Use the ethyl acetate extraction mixed solution,, then under reduced pressure be concentrated into driedly, obtain compound (s) (22.3g, 64.5mmol, the content 85.6% that HPLC (reference example 27) records, yield 95%) the organic layer separatory.
Compound (s):
1H-NMR (400MHz, CDCl
3): δ 1.00 (3H, t, J=7.3Hz, CH
3), 1.02 (3H, t, J=7.3Hz, CH
3), 1.83 (4H, m, CH
2* 2), 3.75 (1H, s, OH), 4.35 (2H, t, J=6.8Hz, CH
2), 5.21 (1H, d, J=17.1Hz), 5.61 (1H, d, J=17.1Hz), 7.28 (1H, s, aromatic ring-H), 9.59 (1H, brs, OH).
(10) compound (t) is synthetic
At room temperature, (0.40g 2.92mmol), under argon atmosphere, stirred 20 minutes in 50 ℃ to add salt of wormwood in DMSO (7mL) solution of compound (s) (0.50g, 1.46mmol, content 86.6%).(2.1mL 14.6mmol), under argon atmosphere, stirred 24 hours in 50 ℃ the dropwise addition of acrylic acid tert-butyl ester in mixture.In ice-cold stirring downhill reaction mixture, add entry (10mL) and concentrated hydrochloric acid (1mL).With the mixed liquid of toluene-ethyl acetate (4: 1,7mL) with mixture extraction 4 times.Merge organic layer, anhydrous sodium sulfate drying is used in water (5mL) washing 3 times, filter, then under reduced pressure be concentrated into dried, acquisition tawny solid chemical compound (t) (0.55g 1.13mmol, the content 75.0% that HPLC (reference example 28) records, yield 77%).
Compound (t):
1H-NMR (400MHz, CDCl
3): δ 0.99 (3H, t, J=7.4Hz, CH
2C
H 3 ), 1.58 (9H, s, t-Bu), 1.83 (2H, m, C
H 2 CH
3), 4.68 (2H, s, CH
2), 5.25 (1H, d, J=17.8Hz), 5.69 (1H, d, J=17.8Hz), 7.01 (1H, s, aromatic ring-H).
(11) compound (h) is synthetic
Under argon atmosphere, under room temperature to compound (t) (1.02g, 1.84mmol, content 66.0%) add trifluoroacetic acid (1.7mL) in the toluene solution (17mL), under argon atmosphere, stirred 100 minutes in 110 ℃, and put and be chilled to room temperature, under reduced pressure be concentrated into dried.In residue, add methylene dichloride (50mL), filter insolubles by the Celite pad.In filtrate, add entry (10mL) and separate organic layer, then in water layer, add methylene dichloride (20mL) extraction 3 times.Merge organic layer, use anhydrous sodium sulfate drying, filter, then under reduced pressure be concentrated into dried, obtain solid chemical compound (h) ((S)-4-ethyl-7,8-dihydro-4-hydroxyl-1H-pyrans-[3,4-f] indolidin-3,6,10 (4H)-triketones) (0.46g, 1.41mmol, the content 80.7% that HPLC records, yield 77%).
Below show the HPLC condition that assay is used and the NMR spectrum of compound (h).
Compound (h):
1H-NMR (400MHz, CDCl
3): δ 0.98 (3H, t, J=7.3Hz, CH
2C
H 3 ), 1.81 (2H, m, C
H 2 CH
3), 2.97 (2H, t, J=6.3Hz, C
H 2 CH
2), 3.64 (1H, s, OH), 4.34 (2H, m, CH
2C
H 2 ), 5.25 (1H, d, J=17.1Hz), 5.68 (1H, d, J=17.1Hz), 7.22 (1H, s, aromatic ring-H).
The HPLC operational condition:
Post: GL Science Inertsil ODS-2 (0.46cm ID * 25cm)
Near temperature: the constant temperature 40 ℃
Flow velocity: 1mL/ minute
Moving phase: 10mM potassium primary phosphate: methyl alcohol mixes liquid (4: 1)
Detect wavelength: 254nm.
[embodiment 31] 7-ethyl-10-hydroxyl CPT's (SN-38) is synthetic
Compound (the h) (0.50g that embodiment 30 (11) are obtained, content 96.6%, 1.82mmol) and compound (e) (0.36g 2.14mmol) is suspended in the mixed liquid of acetic acid-toluene (1: 1,10mL), at room temperature add a hydration tosic acid (p-TsOHH
2O 10mg), stirred 18 hours in 100 ℃ under nitrogen atmosphere.After reaction solution under reduced pressure concentrated, in residue, add toluene (10mL), then under reduced pressure concentrate.At room temperature in residue, add acetone (9mL), stir after 2 hours, filter precipitate, the material that obtains with acetone (2mL * 2) washing and filtering.Drying under reduced pressure obtains brown solid (0.63g, the purity 97.7% that HPLC (reference example 9) records, yield 89%).
SN-38：
1H-NMR(400MHz，CDCl
3)：δ0.98(3H，t，J＝7Hz，CH
3)，1.38(3H，t，J＝7Hz，CH
3)，1.90(2H，q，J＝7Hz，CH
2)，3.08(2H，q，J＝7Hz，CH
2)，5.17(2H，s，CH
2O)，5.23(1H，d，J＝16Hz)，5.54(1H，d，J＝16Hz)，7.34-7.39(3H，m)，6.83(1H，d，J＝9Hz)。
[embodiment 32] 7-ethyl-10-[4-(1-piperidino-(1-position only))-1-piperidino-(1-position only)] carbonyl oxygen base camptothecine hydrochloride (SN-38B-11) synthetic
With the synthetic SN-38 that obtains among the embodiment 31 (0.91g, 2.32mmol), according to existing method (Sawada, S.; Okajima, S.; Aiyama, R.; Nokata, K.; Furuta, T.; Yokokura, T.; Sugino, E.; Yamaguchi, K.; Miyasaka, T., Chem.Pharm.Bull., 1991,39,1446) synthetic SN-38B-11 (chirality HPLC (reference example 10) records 99.8%ee for 1.22g, yield 89%).
[embodiment 33] 7-ethyl-10-[4-(1-piperidino-(1-position only))-1-piperidino-(1-position only)] carbonyl oxygen base happiness hydrochloric acid tree alkali salt hydrochlorate (CPT-11) synthetic
(1.00g, 1.7mmol) the middle hydrochloric acid (20mL) that adds 0.1N makes it dissolving by near heating 80 ℃ to the SN-38B-11 that obtains in embodiment 32, adds acetonitrile (100mL), at room temperature stirs 15 hours.Filter precipitate, drying, moisture absorption obtains yellow-white powder CPT-11 (0.95mg, yield 89.8%).
Industrial applicibility
The synthetic method of the application of the invention can at short notice with 2 ' of high-recovery synthesis of high purity-amino-5 '-hydroxypropiophenone and tricycle kentones, by using them as intermediate, can effectively and practicably be carried out the complete synthetic of CPT class.
Claims (13)
1. the preparation method who is used for 2 ' of synthetic camptothecin-amino-5 '-hydroxypropiophenone, wherein from compound (a):
Generate compound (b):
Generate compound (c) from compound (b):
Generate compound (d) from compound (c):
Generate compound (e) from compound (d):
It is characterized in that R may pass through catalytic reduction and the protecting group of deprotection.
2. according to the method for claim 1, it is characterized in that may be by catalytic reduction the protecting group of deprotection be benzyl.
3. according to the method for claim 1 or 2, it is characterized in that described method comprises one or more following steps that are selected from:
(1) compound (a), benzyl reagent and alkali are mixed, and in solvent this mixture of heated and stirred and obtain the step of compound (b);
(2) under atmosphere of inert gases, in compound (b), drip Grignard reagent and obtain the step of compound (c);
(3) compound (c) and oxygenant are mixed, and stir and the step of acquisition compound (d);
(4) catalytic reduction compound (d) and obtain the step of compound (e).
4. according to the method for claim 3, wherein the solvent in the step (1) is a dimethyl formamide.
5. according to the method for claim 3, wherein the Grignard reagent in the step (2) is a vinyl bromination magnesium.
6. according to the method for claim 3, wherein the oxygenant in the step (3) is Jones reagent, Manganse Dioxide or TEMPO-clorox.
7. the preparation method who is used for 2 ' of synthetic camptothecin-amino-5 '-hydroxypropiophenone, wherein from compound (a):
The generation compound (c "):
Generate compound (d ") from compound (c "):
Generate compound (e) from compound (d "):
8. according to the method for claim 7, it is characterized in that described method comprises one or more following steps that are selected from:
(1) under atmosphere of inert gases, in compound (a), drips Grignard reagent and obtain the step of compound (c ");
(2) compound (c ") and oxygenant are mixed, and stir and obtain the step of compound (d ");
(3) catalytic reduction compound (d ") and obtain the step of compound (e).
9. method according to Claim 8, wherein the Grignard reagent in the step (1) is a vinyl bromination magnesium.
10. according to the method for claim 9, wherein the oxygenant in the step (2) is Jones reagent, Manganse Dioxide or TEMPO-clorox.
11. the purposes that is used to prepare camptothecin of 2 '-amino-5 '-hydroxypropiophenone that each the method by claim 1～10 obtains.
12. the preparation method of camptothecin, described method comprise 2 '-amino-5 '-hydroxypropiophenone and the tricycle kentones reaction that each the method by claim 1～10 is obtained.
13. according to the method for claim 12, wherein tricycle kentones and 2 '-amino-5 '-hydroxypropiophenone is mixed, and under atmosphere of inert gases, reacts this mixture.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001-45430 | 2001-02-21 | ||
| JP200145430 | 2001-02-21 | ||
| JP2001045430 | 2001-02-21 | ||
| JP2001309322 | 2001-10-05 | ||
| JP2001309322 | 2001-10-05 | ||
| JP2001-309322 | 2001-10-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB028053230A Division CN100408550C (en) | 2001-02-21 | 2002-02-21 | Method for synthesizing camptothecin-relating compounds |
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| CN101337900B CN101337900B (en) | 2013-12-18 |
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| US5391745A (en) * | 1992-07-23 | 1995-02-21 | Sloan-Kettering Institute For Cancer Research | Methods of preparation of camptothecin analogs |
| SG116433A1 (en) * | 1996-10-30 | 2005-11-28 | Tanabe Seiyaku Co | S type 2-substituted hydroxy-2-indolidinylbutyric ester compounds and process for preparation thereof. |
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| ZA200306223B (en) | 2004-06-03 |
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