CN101333156B - Preparation method and uses of antrodia camphorate pimelie kelone compounds - Google Patents

Preparation method and uses of antrodia camphorate pimelie kelone compounds Download PDF

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CN101333156B
CN101333156B CN2007101268050A CN200710126805A CN101333156B CN 101333156 B CN101333156 B CN 101333156B CN 2007101268050 A CN2007101268050 A CN 2007101268050A CN 200710126805 A CN200710126805 A CN 200710126805A CN 101333156 B CN101333156 B CN 101333156B
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motion
cinnamomum kanahirai
kanahirai hay
pimelie kelone
compound
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CN101333156A (en
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刘胜勇
温武哲
郭茂田
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GUODING BIOTECHNOLOGY CO Ltd
Golden Biotechnology Corp
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GUODING BIOTECHNOLOGY CO Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/22—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and doubly-bound oxygen atoms bound to the same carbon skeleton
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
    • C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
    • C07C49/753—Unsaturated compounds containing a keto groups being part of a ring containing ether groups, groups, groups, or groups

Abstract

The invention relates to an Antrodia cinnamomea compound which can slow down physiological fatigue, in particular to a 4-hydroxy -2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone separated from the extract of Antrodia cinnamomea, which can effectively slow down the physiological fatigue. In the invention, after 80% of maximal oxygen uptake of high-intensity exhausted exercises, the application of the compound can promote the metabolism of creatine kinase and blood ammonia in the body and contribute to the restoration of the concentration to achieve the effect of slowing down the physical fatigue.

Description

The preparation method of Cinnamomum kanahirai hay pimelie kelone compound and application thereof
Technical field
The invention relates to a kind of compound that is used for antifatigue, especially about a kind of cyclonene compound that makes, is used to slow down physiological fatigue by Antrodia camphorata (Antrodia camphorata) extract separation and purification.
Background technology
Antrodia camphorata (Antrodia camphorata), claim camphor tree sesame, Cinnamomum kanahirai hay mushroom or red camphor tree sesame etc. again, the perennial gill fungus mushroom that belongs to Aphyllophorales (Aphyllophorales) polyporaceae (Polyporaceae), be Taiwan endemic species fungi, only grow on the rotten heartwood inwall of hollow of Taiwan child care class seeds-Cinnamomum kanahirai hay tree (Cinnamoum kanehirai Hay).Because Cinnamomum kanahirai hay tree distributed quantity is very rare, add artificial felling trees unlawfully, make to parasitize more shape rareness of the wild Antrodia camphorata quantity that wherein can grow, and because its sporophore growth is quite slow, vegetative period is also only between June to October, so price is very expensive.
The sporophore of Antrodia camphorata is perennial, and stockless is suberin to wooden, and its tool intensive camphor tree fragrance, and changeableization of form have tabular, mitriform, horse-hof shape or tower shape.Nascent is platypelloid type and is bright red that its periphery can present radiation warp shape afterwards, and to expansion growth all around, color also changes light red brown or khaki into, and many pores are arranged, and is the abundantest position of pharmaceutical use of Antrodia camphorata.
In the Taiwan folk custom medically, Antrodia camphorata has detoxifcation, alleviating diarrhoea symptom, anti-inflammatory, treatment liver related disease and function such as anticancer.Antrodia camphorata is as edible medicinal gill fungus mushroom class; composition with many complexity; in the known physiologically active ingredient; comprise: triterpene compound (triterpenoids); polysaccharide body (polysaccharides; as callose); adenosine (adenosine); VITAMIN is (as vitamins B; niacin); protein (containing immunoglobulin (Ig)); sudismase (superoxide dismutase; SOD); trace element (as: calcium; phosphorus; germanium); nucleic acid; steroid and blood pressure stabilization material (as antodia acid) etc., these physiologically active ingredients are considered to have antitumor; increase immunological competence; antianaphylaxis; disease-resistant bacterium; hypertension; hypoglycemic; decreasing cholesterol; multiple efficacies such as protection liver and antifatigue.
In the numerous compositions of Antrodia camphorata with triterpene compound be studied at most, triterpene compound is the general name that is combined into sexangle or pentagon natural compounds by 30 carbons, the bitter taste of Antrodia camphorata institute tool is promptly mainly from this composition of triterpenes.During nineteen ninety-five, people such as Cherng find to contain in the Antrodia camphorata sporophore extract three kinds new be the triterpene compound of skeleton with ergostane (ergostane): antcin A, antcin B and antcin C (Cherng, I.H., and Chiang, H.C.1995.Three new triterpenoids fromAntrodia cinnamomea.J.Nat.Prod.58:365-371).People such as Chen find three kinds of triterpene compound (Chen such as zhankuic acid A, zhankuic acid B and zhankuic acid C after with alcohol extraction camphor tree sesame sporophore, C.H., and Yang, S.W.1995.New steroid acids from Antrodiacinnamomea ,-a fungus parasitic on Cinnamomum micranthum.J.Nat.Prod.58:1655-1661).In addition, people such as Chiang find other three kinds of new triterpene compounds that are respectively sesquiterpene lactones (sesquiterpene lactone) and two kinds of bisphenols derivatives in also by the sporophore extract in nineteen ninety-five, and this is antrocin, 4,7-dimethoxy-5-methyl isophthalic acid, 3-benzo dioxy ring (4,7-dimethoxy-5-methy-1,3-benzodioxole) with 2,2 ', 5,5 '-tetramethoxy-3,4,3 ', 4 '-two-methylene-dioxy-6,6 '-dimethyl diphenyl (2,2 ', 5,5 '-teramethoxy-3,4,3 ', 4 '-bi-methylenedioxy-6,6 '-dimethylbiphenyl) (Chiang, H.C., Wu, D.P., Cherng, I.W., and Ueng, C.H.1995.A sesquiterpene lactone, phenyl and biphenyl compoundsfrom Antrodia cinnamomea.Phytochemistry.39:613-616).By 1996, people such as Cherng find four kinds of new triterpene compounds once again with same analytical procedure: antcin E, antcin F, methyl antcinate G, methyl antcinate H (Cherng, I.H., Wu, D.P., and Chiang, H.C.1996.Triteroenoids from Antrodia cinnamomea.Phytochemistry.41:263-267); People such as Yang have found that then two kinds is the new compound zhankuic acid D of skeleton with the ergostane, zhankuic acid E, with three kinds be the new compound of skeleton: 15 α-acetyl-dehydrogenation sulfurenic acid (15 α-acetyl-dehydrosulphurenic acid) with lanostane (lanostane), the dehydrogenation eburicoic acid (dehydroeburicoic acid) and sulfurenic acid (dehydrasulphurenic the acid) (Yang that anhydrates, S.W., Shen, Y.C., and Chen, C.H.1996.Steroids and triterpenoids of Antrodiacinnamomea-a fungus parasitic on Cinnamomum micranthum.Phytochemistry.41:1389-1392).
Though can learn that by many experiments at present the Antrodia camphorata extract has aforementioned effect, and its ingredient is also analyzed successively to be gone out, but be those effects which kind of effective constituent in the extract can be facilitated Antrodia camphorata actually, not concrete relevant effective constituent is delivered, but the composition of the contained antifatigue of Antrodia camphorata extract wherein, also remaining further experiment research understands fully, if so can find out the contained real effectively composition of antifatigue in this extract, to help studying the related mechanism of Antrodia camphorata antifatigue, and Antrodia camphorata is applied to human body slows down physiological fatigue and produce greatest help.
Summary of the invention
For understanding in the Antrodia camphorata extract which kind of composition actually has the effect of antifatigue, the present invention is provided the compound of formula (1) structural formula by separation and purification in the Antrodia camphorata extract;
Figure G071C6805020070704D000031
Wherein, X is oxygen (O) or sulphur (S), and Y is oxygen or sulphur; R 1Be hydrogen (H), methyl (CH 3) or (CH 2) m-CH 3, R 2Be hydrogen, methyl or (CH 2) m-CH 3, R 3Be hydrogen, methyl or (CH 2) m-CH 3, m=1-12; N=1-12.
In the compound suc as formula (1) structural formula, be preferably as follows the compound shown in the formula (2):
Figure G071C6805020070704D000032
The compound of formula (2), chemistry 4-hydroxyl-2 by name, 3-dimethoxy-6-methyl-5 (3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-2-cyclonene (4-hydroxy-2,3-dimethoxy-6-methy-5 (3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone), molecular formula is C 24H 38O 4, outward appearance is the pale yellow powder shape, molecular weight is 390.
The cyclonene compound separation purifying of Chinese style of the present invention (1), formula (2) is from the water extract or the organic solvent extraction thing of Antrodia camphorata, organic solvent can comprise alcohols (for example methyl alcohol, ethanol or propyl alcohol), ester class (for example ethyl acetate), alkanes (for example hexane) or haloalkane (for example methyl chloride, monochloroethane), but not as limit, wherein the preferably is an alcohols, and better person is an ethanol.
By the cyclonene compound of aforementioned formula (1), formula (2), the present invention is applied to described compound to slow down on the physiological fatigue, at 80% maximal oxygen uptake (80%VO 2Max) after the depleted motion of high strength, replenish the Cinnamomum kanahirai hay pimelie kelone compound immediately and help creatine kinase (creatine phosphate kinase in the body; CPK), slow down because of the muscle cell damage due to the motion, and improve because of blood ammonia in the blood and pile up central nerve fatigue and the peripheral fatigue phenomenon that causes, and then reach the effect of antifatigue with the metabolism of blood ammonia (Ammonia) and the recovery of concentration thereof.
Below conjunction with figs. is further specified embodiments of the present invention; following cited embodiment is in order to illustrate the present invention; be not in order to limit scope of the present invention; any those skilled in the art; without departing from the spirit and scope of the present invention; when can doing a little change and retouching, so protection scope of the present invention is as the criterion when looking the accompanying Claim person of defining.
Description of drawings
After Fig. 1 carries out the depletion campaign or do not have motion for embodiment of the invention experimenter, replenish placebo or Cinnamomum kanahirai hay pimelie kelone compound, creatine kinase concentration results in the body of each time point respectively; among the figure: do not have motion and give placebo (PR);
Figure G071C6805020070704D000041
Motion is arranged and give placebo (PE); △: do not have motion and give Cinnamomum kanahirai hay pimelie kelone compound (DR); ▲: motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE);
After Fig. 2 carries out the depletion campaign or do not have motion for embodiment of the invention experimenter, replenish placebo or Cinnamomum kanahirai hay pimelie kelone compound, blood sugar concentration result in the body of each time point respectively; among the figure: do not have motion and give placebo (PR);
Figure G071C6805020070704D000042
Motion is arranged and give placebo (PE); △: do not have motion and give Cinnamomum kanahirai hay pimelie kelone compound (DR); ▲: motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE);
After Fig. 3 carries out the depletion campaign or do not have motion for embodiment of the invention experimenter, replenish placebo or Cinnamomum kanahirai hay pimelie kelone compound, ammonia concentration result in the body of each time point respectively; among the figure: do not have motion and give placebo (PR);
Figure G071C6805020070704D000043
Motion is arranged and give placebo (PE); △: do not have motion and give Cinnamomum kanahirai hay pimelie kelone compound (DR); ▲: motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE);
After Fig. 4 carries out the depletion campaign or do not have motion for embodiment of the invention experimenter, replenish placebo or Cinnamomum kanahirai hay pimelie kelone compound, blood lactic acid concn result in the body of each time point respectively; among the figure: do not have motion and give placebo (PR);
Figure G071C6805020070704D000044
Motion is arranged and give placebo (PE); △: do not have motion and give Cinnamomum kanahirai hay pimelie kelone compound (DR); ▲: motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE);
After Fig. 5 carries out the depletion campaign or do not have motion for embodiment of the invention experimenter, replenish placebo or Cinnamomum kanahirai hay pimelie kelone compound, free fatty acids concentration results in the body of each time point respectively; among the figure: do not have motion and give placebo (PR);
Figure G071C6805020070704D000045
Motion is arranged and give placebo (PE); △: do not have motion and give Cinnamomum kanahirai hay pimelie kelone compound (DR); ▲: motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE);
Embodiment
At first get Antrodia camphorata (Antrodia camphorata) mycelium, sporophore or the mixture of the two, utilize known extraction mode, extract, so as to obtaining Antrodia camphorata water extract or organic solvent extraction thing with water or organic solvent.Wherein, organic solvent can comprise alcohols (for example methyl alcohol, ethanol or propyl alcohol), ester class (for example ethyl acetate), alkanes (for example hexane) or haloalkane (for example methyl chloride, monochloroethane), but not as limit.Wherein the preferably is an alcohols, and better person is an ethanol.
Through extraction Antrodia camphorata water extract or organic solvent extraction thing later, can be further by in addition separation and purification of high performance liquid chromatography, again each separatory (fraction) is carried out the biochemical test relevant with antifatigue afterwards.At last, then the separatory at tool antifatigue effect carries out composition analysis, and the composition that may produce the antifatigue effect is further done the biochemical test relevant with antifatigue more respectively.Find promptly that finally the compound suc as formula (1)/formula (2) has the effect of slowing down physiological fatigue among the present invention.
The present invention for convenience of description below will be with the 4-hydroxyl-2 of formula (2), and 3-dimethoxy-6-methyl-5 (3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-2-cyclonene compound describes.In addition, for confirming 4-hydroxyl-2,3-dimethoxy-6-methyl-5 (3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-2-cyclonene compound tool antifatigue effect passes through among the present invention to detect through 80% maximal oxygen uptake (80%VO 2Max) tired indexs such as the intravital creatine kinase of depleted post exercise experimenter, blood lactic acid, blood sugar, blood ammonia and free fatty acids are to record the anti-fatigue ability of Cinnamomum kanahirai hay pimelie kelone compound.The result confirms 4-hydroxyl-2 by those biochemical tests, and 3-dimethoxy-6-methyl-5 (3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-2-cyclonene compound tool slows down the effect of physiological fatigue due to the motion back.Now aforementioned embodiments is elaborated as follows:
Embodiment 1:
4-hydroxyl-2, the separation of 3-dimethoxy-6-methyl-5 (3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-2-cyclonene
With Antrodia camphorata mycelium, sporophore or the mixture of the two about 100 grams, insert in the triangular pyramidal bottle, the water and the alcohols (for example alcohol solution more than 70%) that add suitable proportion, stir extraction down more than at least 1 hour in 20-25 ℃, with filter paper and 0.45 μ m membrane filtration, collect extraction liquid afterwards.
Antrodia camphorata extraction liquid with aforementioned collection, utilize high performance liquid chromatograph (High PerformanceLiquid chromatography), chromatographic column (column) with RP18 is analyzed, and with methyl alcohol (A) and 0.1%-0.5% aqueous acetic acid (B) (its solution proportion is: 0～10 minute, the B ratio was 95%～20% as moving phase (mobile phase); 10～20 minutes, the B ratio was 20%～10%; 20～35 minutes, the B ratio was 10%～10%; 35～40 minutes, the B ratio was 10%～95%), wash-out under the speed of per minute 1ml is simultaneously with the long detector analysis of ultraviolet-visible light all-wave.
With 25 minutes to 30 minutes elutriants collect concentrate get final product the solid product of pale yellow powder shape, this is a 4-hydroxyl-2,3-dimethoxy-6-methyl-5 (3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-2-cyclonene.By analysis, its molecular formula is C 24H 38O 4, molecular weight 390, fusing point (m.p.) are 48 ℃-52 ℃.Nucleus magnetic resonance (NMR) analytical value is as follows: 1H-NMR (CDCl3) δ (ppm): 1.51,1.67,1.71,1.75,1.94,2.03,2.07,2.22,2.25,3.68,4.05,5.07 and 5.14.13C-NMR (CDCl3) δ (ppm): 12.31,16.1,16.12,17.67,25.67,26.44,26.74,27.00,39.71,39.81,4.027,43.34,59.22,60.59,120.97,123.84,124.30,131.32,135.35,135.92,138.05,160.45 and 197.12.
Embodiment 2:80% maximal oxygen uptake (80%VO 2Max) exercise load test
Replenish of the influence of Cinnamomum kanahirai hay pimelie kelone compound for recording depleted motion back for anti-fatigue ability, this test utilizes 80% maximal oxygen uptake exercise load to carry out follow-up depletion campaign, so need record maximal oxygen uptake earlier, further to extrapolate the speed of 80% maximal oxygen uptake.Wherein, oxygen uptake is meant the product of cardiac output and arteriovenous blood oxygen concentration difference, and maximal oxygen uptake is meant that a people is on sea level, when being engaged in the most violent motion, the histocyte per minute can consume or utilize the maximum of oxygen, it is for estimating the optimal parameter of cardiorespiratory Endurance, the present invention is with direct measurement in the laboratory, utilize the running in place machine, load when largest motion is loaded with incremental motion, directly measure maximal oxygen uptake, the motion of the gradual incremental motion load of this kind persistence with gas analyzer, can bring out actual maximal oxygen oxygen ability, so it is a method of directly accurately measuring maximal oxygen uptake.
(1) experimenter's basic document is collected
The healthy voluntary male sex was a tested object over 20 years old with 15 in the present invention, medicine, normal, the no cardiovascular disorder of hepatic and renal function are not taken in screening, and do not have smoking on ordinary days, drink, reach and use accessory substance custom person, and every experimenter's of measurement record master data comprises: age, height, body weight and BMI value.The experimenter needs eight hours on an empty stomach at least before carrying out official testing, and need keep normal diet kenel in test period, and avoids taking nutritious supplementary or other medicines, to avoid influencing experimental data.
The exercise load test of (2) 80% maximal oxygen uptakes
The experimenter must be through the secondary before measurement, so as to obtaining maximal oxygen uptake (VO before official testing 2Max), and estimate the exercise load of 80% maximal oxygen uptake, carry out the affirmation test of one time 80% maximal oxygen uptake again, guaranteeing under this specific load being experimenter's the exercise intensity of 80% maximal oxygen uptake, and its testing process details are as follows.
At first carry out the maximal oxygen uptake test, before the experimenter arrived at, (VmaxSpectra, oxygen amount SensorMedics) and the standard gas of different concns were proofreaied and correct to need advanced promoting the circulation of qi body analyser.After treating that the experimenter arrives at, make it put on heartbeat detection table (Polar810i) and write down quiet cardiac rate, and the heartbeat detection band is placed near the locating of experimenter's heart, and the detection table placed apart from detecting be with in 1 meter.The experimenter is prior to running in place machine (Vision, T8600) the 3-5 minute warm body of jogging on adapts to, and make stretching voluntarily, then allow the experimenter stand on the treadmill, put on the gas production face shield, and the gas production face shield is connected with respiratory siphon, and this respiratory siphon and gas analyzer interlink, can be through gas production face shield and respiratory siphon and be sent in the gas analyzer so as to making gas that the experimenter breathes out.Then the running speed with treadmill is fixed on 9.6km/hr, and in 0～3 minute of test beginning, treadmill is set at 0% gradient, begin to test 3 minutes after, needed to increase treadmill 3% gradient, and be stop motion in per 3 minutes up to experimenter's depletion.After the data of analytical gas analyser, obtain the depleted preceding oxygen uptake of preceding 1 minute of each uphill slope degree and motion, and the maximum value of the oxygen uptake of gained is maximal oxygen uptake in the test.In addition, the judging criterion that reaches maximal oxygen uptake must meet the binomial in the following condition at least simultaneously, can be judged to be maximal oxygen uptake: (a) experimenter to full capacity can't persistent movement test (experimenter's step is slack-off can't to advance smoothly with changeing band); (b) cardiac rate reaches (220-age) ± 10 time/minute; (c) (respiratory quotient RQ) must be greater than more than 1.1 for respiratory quotient; (d) (rating perceivedexertion RPE) has reached stage of 18 or 19 to conscious scale.
The maximal oxygen uptake by the before measurement gained and the regression equation of load intensity can further be tried to achieve the speed of 80% maximal oxygen uptake, the oxygen-consumption that this computation process is obtained in testting by maximal oxygen uptake and the data of speed, and try to achieve the tropic of speed (ordinate zou) and oxygen uptake (X-coordinate), again individual maximal oxygen intake be multiply by 80%, oxygen uptake when trying to achieve 80% maximal oxygen uptake, the speed of relative movement of this point is found out in setting-out again.And need to move 10 minutes with this load intensity again, and measured 5-6 minute and last 1 minute oxygen uptake, to determine 80% real maximal oxygen uptake exercise load.Its result as shown in Table 1.
Table one, experimenter's master data and maximal oxygen uptake thereof
Project survey value (mean number ± standard deviation)
Age (year) 22.8 ± 0.89
Height (rice) 1.75 ± 0.01
Body weight (kilogram) 67.73 ± 1.81
BMI(kg×m -2) 22.14±0.63
Maximal oxygen uptake (ml/min/kg) 50.22 ± 0.17
80% maximal oxygen uptake speed 7.61 ± 1.87
n＝15
Embodiment 3: the antifatigue test of Cinnamomum kanahirai hay pimelie kelone compound
Muscle fatigue extensively is defined as individual physiological process and can not keeps its function at certain level in the motion, or each organ can not be kept predetermined exercise intensity, cause the reason of body movement fatigue can comprise psychology, physiology and biochemical three aspects, wherein, biochemical tired potential mechanism comprises maincenter and peripheral tired two aspects, cause the mechanism of central nerve fatigue to comprise hypoglycemia (hypoglycemia), the change of nerve conduction material concentration in the change of key amino acid concentration and the brain in the blood, peripheral tired mechanism then has the phosphocreatine (phospocreatine in the muscle; PC) exhaust and cause that blood ammonia increases, the glycogen in the muscle exhausts and the not enough homenergic shortage phenomenon of oxygen supply, and the hydrogen ion in the muscle is piled up that the lactic acid that is caused increases, the metabolites such as phosphoric acid accumulation in the muscle are piled up factor.
Therefore, the present invention is by giving the placebo that the experimenter is contained the Cinnamomum kanahirai hay pimelie kelone compound and do not contained the Cinnamomum kanahirai hay pimelie kelone compound respectively, and make the experimenter carry out the depletion campaign that intensity is aforementioned 80% maximal oxygen uptake exercise load, simultaneously before motion and the content of tired indexs such as the intravital creatine kinase of motion post analysis experimenter, blood lactic acid, blood sugar, blood ammonia and free fatty acids, and record in the anti-fatigue effect of the afterwards additional Cinnamomum kanahirai hay pimelie kelone compound of depletion campaign.
Give experimenter 4-hydroxyl-2 at random, 3-dimethoxy-6-methyl-5 (3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-placebo of 2-cyclonene compound and non-Cinnamomum kanahirai hay pimelie kelone compound, and the placebo composition can be the material of W-Gum any non-Cinnamomum kanahirai hay pimelie kelone compounds such as (corn starch), and double blinding cross-over design is adopted in this test, and experimenter and testing person be neither to know the material composition that is given.Continue it, based on order balance (order balance) principle, every experimenter all need accept following each group test: do not have motion and give placebo (PR), motion is arranged and give placebo (PE), do not have motion and give Cinnamomum kanahirai hay pimelie kelone compound (DR) and motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE), and the experimenter who accepts different group tests is after finishing one-week this group test, need after the week of having a rest, proceed next group test again, all finish above-mentioned four groups of tests up to every experimenter.Wherein, to take exercises be that intensity (7.61 ± 1.87) with the aforementioned 80% maximal oxygen uptake exercise load that records is carried out on treadmill, and run reach motion depleted (exhausted) to the experimenter till, and after motion, give experimenter's Cinnamomum kanahirai hay pimelie kelone compound or placebo; In addition, the administered dose of placebo is gram/body weight every day 0.2 (kilogram), and the administered dose of Cinnamomum kanahirai hay pimelie kelone compound is gram/body weight every day 0.2 (kilogram), test was carried out seven days by a definite date, and before motion, depleted motion back the 0th, 0.5,1,2,24,48,72, carried out venous blood collection in 120 and 168 hours, blood is collected with the collection tube that contains anti-coagulant (as: EDTA), in 3000xg centrifugal 10 minutes, get blood plasma partly, and carry out follow-up creatine kinase, blood lactic acid, blood sugar, the biochemical analysis of tired index such as blood ammonia and free fatty acids, and give the physiology of different supplementation material and the variation of biochemical values before the comparing motion and after the motion; All biochemical analysis numerical value are all with mean number and standard deviation (Mean ± SEM) expression, and those analyze the difference calibrating that numerical value need to organize with the amount of repetition number two-factor analysis of variance (repeated measurement two way ANOVA) each blood sampling point between interior, group, and with Du Kai Shi afterwards relative method (Tukey ' s honestlysignificant difference) compare afterwards, and the threshold value that reaches conspicuous level after the statistics is decided to be α=0.05, and the analysis mode of those biochemical values and result thereof details are as follows:
(1) creatine kinase (creatine phosphate kinase; CPK) analysis
Organs such as human skeletal muscle, cardiac muscle, brain and prostate gland all can produce serum creatine kinase, and are wherein the abundantest with skeletal muscle content, account for whole body total amount 96% more than; During normal circumstances, creatine kinase activity in the serum is very low, and in when motion Muscle contraction process, but creatine kinase catalysis Triphosaden (adenosinetriphosphate, ATP) with phosphocreatine (phosphate creatine, PC) high-energy phosphate bond between, its chemical equation is:
Figure G071C6805020070704D000091
The resynthesis that Muscle contraction institute's energy requirement can be provided when guaranteeing strenuous exercise fast and promote ATP.And the reason that can impel creatine kinase activity to increase in the moving process, be since when motion anoxic, make meta-bolites pile up, the inside and outside calcium ion of cell is constant unbalance, the permeability that causes muscle cell membrane increases, cause muscle cell membrane sustain damage myotasis for example physical abuse or produce hemotoncus, and then impel creatine kinase to be released into blood circulation, so creatine kinase is often used as the index enzyme that exercise intensity and muscle cell damage.
The present invention measures the method for creatine kinase concentration in the blood, is to utilize dry type automated blood analysis survey meter (Johnson ﹠amp; Johnson DT-60 II), and by the principle of enzyme effect and colorimetric estimation record.After in quantitative blood plasma, adding phosphocreatine glucose oxidase (creatine phosphate glucose oxidase) reaction, add 4-aminoantipyrene (4-aminoantipyrine) and 1 again, 7-dihydroxy naphthlene (1,7-dihydroxynaphthalene), after superoxide enzyme (peroxidase) effect, produce the whitening compound, and measure down its absorbancy in the 680nm wavelength, convert again creatine kinase concentration, result such as table two and shown in Figure 1.
Table two, experimenter carry out the depletion campaign or do not have motion and replenish placebo or Antrodia camphorata cyclonene chemical combination respectively
Behind the thing, the creatine kinase concentration (U/L) of each time point
Figure G071C6805020070704D000101
N=15, numerical value is represented with " mean number ± standard deviation "
* represent p＜.05: with before measurement than variant existence; A represents p＜.05: significantly greater than the PR group with time point
B represents p＜.05: significantly greater than the PE group with time point; C represents p＜.05: significantly greater than the DR group with time point
D represents p＜.05: significantly greater than the DE group with time point
By table two and Fig. 1 as can be known, after carrying out the depleted motion of high strength of 80% maximal oxygen uptake, the group of moving and giving placebo (PE) is arranged in back 0,0.5,1,2 and 24 hour of motion, its creatine kinase concentration value just continue to increase and all significantly (p＜0.05) be higher than the creatine kinase concentration when quiet before the motion, this also represents that experimenter's exercise intensity has reached the muscle injury degree; Motion is arranged simultaneously and give creatine kinase concentration value shown in the group of placebo (PE) significantly (p＜0.05) be higher than and do not have motion and give placebo (PR) and do not have motion and give numerical value shown in Cinnamomum kanahirai hay pimelie kelone compound (DR) two groups, and these two groups of groups without depletion campaign (PR and DR) did not just have the difference existence in back 120 hours up to motion.
Motion also gives the group of Cinnamomum kanahirai hay pimelie kelone compound (DE), and after high-intensity depleted motion, its creatine kinase concentration only rises after motion immediately, the concentration level before back 0.5 hour back of motion promptly returns to motion.In addition, after having the group of moving and giving placebo (PE) to carry out the depletion campaign, its creatine kinase concentration (p＜0.05) significantly is higher than the group that the motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE), and this kind concentration difference is by carrying out continuing to back 1 hour of motion after the depletion campaign, and still can be observed this phenomenon in back 72 hours in motion, this result shows, after the depletion campaign, replenish the Cinnamomum kanahirai hay pimelie kelone compound immediately, can effectively reduce the creatine kinase concentration of representing the muscle injury index, help to slow down the physiological fatigue that the motion back is produced.
(2) analysis of AC Sugar (Blood glucose)
During motion, the secretion of the release of calcium ion and suprarenin (epinephrine) when following Muscle contraction, can activate Starch phosphorylase (phosphorylase) to increase glycogen rate of decomposition in the muscle, the suprarenin of Zeng Jiaing also makes liver quicken glycogen decomposition reaction (glycogenolysis) simultaneously, and then makes blood sugar concentration increase in the blood.Often lead because of the sustainable utilization in the oxidative metabolism process of blood sugar in body in the fatigue that the depleted motion of the high strength of 80% maximal oxygen uptake is produced down, produce hypoglycemic phenomenon, and cause exhausting of glycogen in the muscle, therefore, intravital carbohydrate utilizes situation when detecting motion, can be used as one of index of observing physiological fatigue.
The present invention measures the method for blood sugar concentration, utilizes dry type automated blood analysis survey meter (Johnson ﹠amp; Johnson DT-60 II), and by the principle of enzyme effect and colorimetric estimation record.After in quantitative blood plasma, adding glucose oxidase (glucose oxidase) reaction, add 4-aminoantipyrene (4-aminoantipyrine) and 1 again, 7-dihydroxy naphthlene (1,7-dihydroxynaphthalene), produce red compound after superoxide enzyme (peroxidase) effect, its reaction formula is as follows:
Figure G071C6805020070704D000111
Figure G071C6805020070704D000121
And measure down its absorbancy in the 555nm wavelength, convert again blood sugar concentration, result such as table three and shown in Figure 2.
Table three, experimenter carry out the depletion campaign or do not have motion and replenish placebo or Antrodia camphorata cyclonene chemical combination respectively
Behind the thing, the blood sugar concentration of each time point (mg/dl)
Figure G071C6805020070704D000122
N=15, numerical value is represented with " mean number ± standard deviation "
* represent p＜.05: with before measurement than variant existence; A represents p＜.05: significantly greater than the PR group with time point
B represents p＜.05: significantly greater than the PE group with time point; C represents p＜.05: significantly greater than the DR group with time point
D represents p＜.05: significantly greater than the DE group with time point
By table three and Fig. 2 as can be known, motion is arranged and give placebo (PE) and motion is arranged and give the group of Cinnamomum kanahirai hay pimelie kelone compound (DE), after the depleted motion of the high strength of 80% maximal oxygen uptake, its blood glucose concentration value is with the nothing motion and give placebo (PR) and do not have motion and give Cinnamomum kanahirai hay pimelie kelone compound (DR) group that (p＜0.05) difference is arranged significantly; In addition, have the motion and give placebo (PE) and have the motion and give Cinnamomum kanahirai hay pimelie kelone compound (DE) two groups, level before the back 0.5 hour AC Sugar concentration of the depleted motion of the high strength of 80% maximal oxygen uptake all can return to not motion and near rest value, this result shows, replenishes the metabolism did not influence of camphor tree pimelie kelone compound to AC Sugar in the motion back.
(3) analysis of blood ammonia (Ammonia)
Blood ammonia is proteinic meta-bolites, and it mainly is because the interior purine nuclear of body circulation (purine nucleotidecycle; PNC) amino acid whose decomposition and gland purine vouching phosphoric acid (adenosine monophosphate in; AMP) deamination forms; and when motion exhausting because of phosphocreatine in the body (creatine phosphate); make the adenine nucleotide (adeninenucleotide) in the tissue decompose; to quicken the resynthesis of ATP; can produce a large amount of blood ammonias this moment; and the increase of ammonia concentration can change the effect of central nervous system; make extracellular pH value; electrolyte concentration and nerve conduction material concentration are changed; and interference Ke Shi circulation (Krebscycle); cause tired generation; therefore, it also is to cause one of central nerve fatigue and peripheral tired factor that blood ammonia is piled up, so blood ammonia regular meeting is used as one of tired index.
The present invention measures the method for ammonia concentration, utilizes dry type automated blood analysis survey meter (Johnson ﹠amp; Johnson DT-60 II), and the principle by colorimetric estimation, in quantitative blood plasma, adding photoghraphic coupler tetrabromophenol sulfonphthalein (bromphenol blue), the effect back produces blue compound, and its reaction formula is as follows:
NH 3+ tetrabromophenol sulfonphthalein → blue dyes (Blue dye) is also measured its absorbancy down in the 605nm wavelength, convert again ammonia concentration, result such as table four and shown in Figure 3.
Table four, experimenter carry out the depletion campaign or do not have motion and replenish placebo or Antrodia camphorata cyclonene chemical combination respectively
Behind the thing, the ammonia concentration of each time point (μ g/dl)
Figure G071C6805020070704D000131
N=15, numerical value is represented with " mean number ± standard deviation "
* represent p＜.05: with before measurement than variant existence; A represents p＜.05: significantly greater than the PR group with time point
B represents p＜.05: significantly greater than the PE group with time point; C represents p＜.05: significantly greater than the DR group with time point
D represents p＜.05: significantly greater than the DE group with time point
By table four and Fig. 3 as can be known, motion is arranged and give placebo (PE) and motion is arranged and give the group of Cinnamomum kanahirai hay pimelie kelone compound (DE), after the depleted motion of the high strength of 80% maximal oxygen uptake, its ammonia concentration value (p＜0.05) significantly is higher than the nothing motion and gives placebo (PR) and do not have the group of moving and giving Cinnamomum kanahirai hay pimelie kelone compound (DR).Motion is arranged and give placebo (PE) and motion is arranged and give the ammonia concentration of two groups of Cinnamomum kanahirai hay pimelie kelone compounds (DE), promptly recover gradually back 1 hour of motion, and the ammonia concentration that records before the ammonia concentration numerical value of this time point and motion there is no significantly after relatively, and the difference of (p＞0.05) exists; And in back 2 hours of motion, motion is arranged and give Cinnamomum kanahirai hay pimelie kelone compound (DE) group and (p＜0.05) difference significantly appears in the ammonia concentration numerical value that motion is arranged and give between placebo (PE) group on statistics.In addition, by also can be observed among table four and Fig. 3, in back 24 hours of motion, motion is arranged and give ammonia concentration that Cinnamomum kanahirai hay pimelie kelone compound (DE) group shows before (p＜0.05) is lower than not motion significantly, and in this identical time point, the same ammonia concentration that moves and give placebo (PE) group through having of depleted motion is similar to the numerical value before the not motion, there is no and the significantly reduced effect of above-mentioned ammonia concentration occurs, therefore, show by The above results, replenish the Cinnamomum kanahirai hay pimelie kelone compound in the motion back and help the metabolism of blood ammonia, but be piled up in intravital blood ammonia tachymetabolism after making motion, and then slow down the physiological fatigue that depleted motion back is produced.
(4) analysis of blood lactic acid (Lactate)
Lactic acid is that glycogen in muscle and the liver, glucose are separated the product that the effect metabolism forms through anaerobic sugar, under quiet state, the growing amount of lactic acid is less, when fierceness or prolonged exercise, the histanoxia situation is more obvious, anaerobic metabolism speeds up, and when the generating rate of lactic acid is higher than the speed of a line body oxidative metabolism lactic acid, will cause the lactic acid of increase to be piled up in the muscle gradually.The accumulation of lactic acid can impel hydrogen ion concentration rising, the pH value in the muscle to descend, this can suppress glucolytic phosphofructokinase (phosphofructokinase on the contrary, PFK) activity, cause sugar to separate that the speed of action weakens and ATP resynthesis rate reduces,, influence the release and the myofibrillar contractility of reduction of calcium ion in the sarcoplasmic reticulum in addition because of hydrogen ion concentration increases, so accumulation that lactic acid is a large amount of, the generation that will accelerate fatigue and react, therefore, blood lactic acid is often used as one of tired index.
The present invention measures the method for blood lactic acid concn, utilizes dry type automated blood analysis survey meter (Johnson ﹠amp; Johnson DT-60 II), and the principle by enzyme effect and colorimetric estimation, after in quantitative blood plasma, adding Lactate Oxidase (lactate oxidase) reaction, add 4-aminoantipyrene (4-aminoantipyrine) and 1 again, 7-dihydroxy naphthlene (1,7-dihydroxynaphthalene), produce red compound after superoxide enzyme (peroxidase) effect, its reaction formula is as follows:
Figure G071C6805020070704D000151
And measure down its absorbancy in the 540nm wavelength, convert again the blood lactic acid concn, result such as table five and shown in Figure 4.
Table five, experimenter carry out the depletion campaign or do not have motion and replenish placebo or Antrodia camphorata cyclonene chemical combination respectively
Behind the thing, the blood lactic acid concn (mmol/l) of each time point
Figure G071C6805020070704D000153
N=15, numerical value is represented with " mean number ± standard deviation "
* represent p＜.05: with before measurement than variant existence; A represents p＜.05: significantly greater than the PR group with time point
B represents p＜.05: significantly greater than the PE group with time point; C represents p＜.05: significantly greater than the DR group with time point
D represents p＜.05: significantly greater than the DE group with time point
By table five and Fig. 4 as can be known, motion is arranged and give placebo (PE) and motion is arranged and give the group of Cinnamomum kanahirai hay pimelie kelone compound (DE), in back 0.5 hour of the depleted motion of the high strength of 80% maximal oxygen uptake, its blood lactic acid concn value (p＜0.05) significantly is higher than and does not have motion and give placebo (PR) and do not have motion and give the group of Cinnamomum kanahirai hay pimelie kelone compound (DR), and this has the intervention of exercise intensity of two groups of groups of motion (PE and DE), and also all there were significant differences (p＜0.05) with blood lactic acid concn value that no motion group (PR and DR) is presented.Motion is arranged and give placebo (PE) and motion is arranged and give the ammonia concentration of two groups of Cinnamomum kanahirai hay pimelie kelone compounds (DE), promptly recovered gradually in back 1 hour also near the rest value before the motion in motion, and the blood lactic acid concn that records before the ammonia concentration numerical value of this time point and motion there is no significant difference after relatively and has (p＞0.05), this result shows, replenishes the metabolism did not influence of camphor tree pimelie kelone compound to blood lactic acid in the motion back.
(5) analysis of free fatty acids (free fatty acid)
The generation of free fatty acids is by the fatty tissue between myofiber, or triglyceride hydrolysis in the cell.During endurance exercise, be stored in the muscle glycogen can with exhaust, and the time that continues along with motion increases, sugar is separated the speed of action and will be weakened gradually, the utilization of energy i (in vivo) is tending towards the utilization of lipid acid, this moment, the generation of ATP just reduced, thereby caused fatigue; In addition, when steatolysis speed improves, make when free fatty acid concentration increases in the blood plasma, free fatty acids can with tryptophane in the blood (Tryptophan) vie each other binding site on the albumin (Albumin), cause free tryptophane increase in the blood, tryptophane is the precursor of thrombotonin, when tryptophane enters in the brain, the thrombotonin synthesis rate is increased.When thrombotonin in the brain increases, can damage the action system of nervus centralis aspect, as: reduce the activity of Dopamine HCL (Dopamine), also can cause tired generation and sleep response, therefore, the concentration of free lipid acid can be used as steatolysis and produces tired index in the blood.
The present invention measures the method for free-fat acid concentration; utilize the principle of enzyme effect and colorimetric estimation; in quantitative blood plasma, be incorporated in add acyl-CoA synthetase (acyl CoA synthetase) in the quantitative blood plasma, ACOD (acyl CoA oxidase) reacts it; produce the purple compound again after superoxide enzyme (peroxidase) effect, its reaction formula is as follows:
Figure G071C6805020070704D000171
Figure G071C6805020070704D000172
Figure G071C6805020070704D000173
The 4-AAP:4-aminoantipyrene
TOOS:N-ethyl-N-(2-hydroxyl-3-sulfo group propyl group) m-Tolylamine
(N-ethyl-N-(2-hydroxy-3-sulphopropyl)m-toluidine)
And measure down its absorbancy in the 550nm wavelength, convert again the blood lactic acid concn, result such as table six and shown in Figure 5.
Table six, experimenter carry out the depletion campaign or do not have motion and replenish placebo or Antrodia camphorata cyclonene chemical combination respectively
Behind the thing, the free-fat acid concentration of each time point (μ mol/l)
Figure G071C6805020070704D000174
N=15, numerical value is represented with " mean number ± standard deviation "
* represent p＜.05: with before measurement than variant existence; A represents p＜.05: significantly greater than the PR group with time point
B represents p＜.05: significantly greater than the PE group with time point; C represents p＜.05: significantly greater than the DR group with time point
D represents p＜.05: significantly greater than the DE group with time point
By table six and Fig. 5 as can be known, motion is arranged and give placebo (PE) and motion is arranged and give the group of Cinnamomum kanahirai hay pimelie kelone compound (DE), high strength depletion in 80% maximal oxygen uptake was moved back 2 hours in, and the fatty acid concentration value of dissociating in its blood (p＜0.05) significantly is higher than the nothing motion and gives placebo (PR) and do not have the group of moving and giving Cinnamomum kanahirai hay pimelie kelone compound (DR).All there is not significant difference (p＞0.05) and motion is arranged and give placebo (PE) and motion is arranged and give two groups of Cinnamomum kanahirai hay pimelie kelone compounds (DE) the fatty acid concentration value of in the measured blood of each time test point of post exercise, dissociating, this result shows, in the motion back replenish the camphor tree pimelie kelone compound to blood in the metabolism did not influence of free lipid acid.
This in sum, inventive result is replenished the camphor tree pimelie kelone compound after being presented at the depleted motion of high strength of 80% maximal oxygen uptake immediately, the concentration of creatine kinase only significantly rises after motion immediately, 0.5 its creatine kinase concentration promptly returns to level before the motion after hour, this result is presented at and replenishes the camphor tree sesame after the depleted motion immediately and have significantly (p＜0.05) effect for the metabolism of creatine kinase; And in the metabolism of blood ammonia after the motion, the group of replenishing camphor tree pimelie kelone compound (DE) after the depleted motion immediately back 2 hours in motion, its recovery effects significantly (p＜0.05) are better than not replenishing the group of camphor tree pimelie kelone compound (PE); In addition, tired indexs such as blood sugar, blood lactic acid and free fatty acids do not have significant difference between two groups of the Cinnamomum kanahirai hay pimelie kelone compounds (DE) motion being arranged and give placebo (PE) and motion is arranged and give.Therefore, after the depleted motion of the high strength of 80% maximal oxygen uptake, replenish the recovery that the camphor tree pimelie kelone compound helps creatine kinase and blood ammonia in the body immediately, and then reach the effect of slowing down physiological fatigue.

Claims (7)

1. the preparation method of Cinnamomum kanahirai hay pimelie kelone compound, wherein, described Cinnamomum kanahirai hay pimelie kelone compound is made by the organic solvent extraction thing separation of Antrodia camphorata, described Cinnamomum kanahirai hay pimelie kelone compound is a 4-hydroxyl-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-the 2-cyclonene, described organic solvent is selected from the group of being made up of ester class, alcohols, alkanes or haloalkane.
2. the preparation method of Cinnamomum kanahirai hay pimelie kelone compound according to claim 1, wherein, described alcohols is an ethanol.
3. the preparation method of Cinnamomum kanahirai hay pimelie kelone compound is characterized in that comprising the steps:
With Antrodia camphorata mycelium, sporophore or the mixture of the two of 100 grams, insert in the triangular pyramidal bottle, add the alcohol solution more than 70%, stir extraction more than at least 1 hour down in 20-25 ℃, afterwards with filter paper and 0.45 μ m membrane filtration, collect extraction liquid;
Extraction liquid with aforementioned collection, utilize high performance liquid chromatograph, analyze with the RP18 chromatographic column, and with methyl alcohol and 0.1%-0.5% aqueous acetic acid as moving phase, wherein, the ratio of described methyl alcohol and 0.1%-0.5% aqueous acetic acid is: 0～10 minute, the ratio of described 0.1%-0.5% aqueous acetic acid was 95%～20%; 10～20 minutes, the ratio of described 0.1%-0.5% aqueous acetic acid was 20%～10%; 20～35 minutes, the ratio of described 0.1%-0.5% aqueous acetic acid was 10%～10%; 35～40 minutes, the ratio of described 0.1%-0.5% aqueous acetic acid was 10%～95%, wash-out under the speed of per minute 1ml;
With 25 minutes to 30 minutes elutriants collect concentrate get final product 4-hydroxyl-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-2-cyclonene.
4. the Cinnamomum kanahirai hay pimelie kelone compound slows down application in the medicine of physiological fatigue in preparation, wherein, described Cinnamomum kanahirai hay pimelie kelone compound is a 4-hydroxyl-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-and the 2-cyclonene, described Cinnamomum kanahirai hay pimelie kelone compound is antifatigue by the metabolism that promotes creatine kinase in the body of motion back.
5. Cinnamomum kanahirai hay pimelie kelone compound according to claim 4 slows down application in the medicine of physiological fatigue in preparation, and wherein, described motion is the depleted motion of the high strength of 80% maximal oxygen uptake.
6. the Cinnamomum kanahirai hay pimelie kelone compound slows down application in the medicine of physiological fatigue in preparation, wherein, described Cinnamomum kanahirai hay pimelie kelone compound is a 4-hydroxyl-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethylammonium-2,6,10-12 carbon triolefins)-and the 2-cyclonene, described Cinnamomum kanahirai hay pimelie kelone compound is antifatigue by the metabolism that promotes blood ammonia in the body of motion back.
7. Cinnamomum kanahirai hay pimelie kelone compound according to claim 6 slows down application in the medicine of physiological fatigue in preparation, and wherein, described motion is the depleted motion of the high strength of 80% maximal oxygen uptake.
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