CN101326289A - Method for determination of cholesterol level in remnant-like lipoprotein, reagent and kit - Google Patents
Method for determination of cholesterol level in remnant-like lipoprotein, reagent and kit Download PDFInfo
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- CN101326289A CN101326289A CNA2006800464391A CN200680046439A CN101326289A CN 101326289 A CN101326289 A CN 101326289A CN A2006800464391 A CNA2006800464391 A CN A2006800464391A CN 200680046439 A CN200680046439 A CN 200680046439A CN 101326289 A CN101326289 A CN 101326289A
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- reagent
- surfactant
- cholesterol
- hydrogen peroxide
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title claims abstract description 106
- 238000000034 method Methods 0.000 title claims abstract description 56
- 102000004895 Lipoproteins Human genes 0.000 title claims abstract description 43
- 108090001030 Lipoproteins Proteins 0.000 title claims abstract description 43
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 1298
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 750
- 239000005515 coenzyme Substances 0.000 claims abstract description 575
- 239000004094 surface-active agent Substances 0.000 claims abstract description 416
- 102000000019 Sterol Esterase Human genes 0.000 claims abstract description 369
- 108010055297 Sterol Esterase Proteins 0.000 claims abstract description 369
- 108010023417 cholesterol dehydrogenase Proteins 0.000 claims abstract description 292
- 108010085346 steroid delta-isomerase Proteins 0.000 claims abstract description 292
- 238000006243 chemical reaction Methods 0.000 claims abstract description 99
- 239000012736 aqueous medium Substances 0.000 claims abstract description 61
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 53
- -1 alkyl ethers phosphoric acid ester Chemical class 0.000 claims description 272
- 238000012360 testing method Methods 0.000 claims description 257
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- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 256
- 239000013543 active substance Substances 0.000 claims description 249
- 238000005691 oxidative coupling reaction Methods 0.000 claims description 178
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 167
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 claims description 141
- 229920000447 polyanionic polymer Polymers 0.000 claims description 138
- 238000013098 chemical test method Methods 0.000 claims description 83
- 229910021645 metal ion Inorganic materials 0.000 claims description 65
- 125000000217 alkyl group Chemical group 0.000 claims description 56
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 40
- 238000011161 development Methods 0.000 claims description 36
- USIUVYZYUHIAEV-UHFFFAOYSA-N diphenyl ether Chemical compound C=1C=CC=CC=1OC1=CC=CC=C1 USIUVYZYUHIAEV-UHFFFAOYSA-N 0.000 claims description 32
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 30
- 125000005037 alkyl phenyl group Chemical group 0.000 claims description 28
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- 150000005215 alkyl ethers Chemical class 0.000 claims description 21
- 150000001336 alkenes Chemical class 0.000 claims description 18
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical class C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 claims description 16
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 claims description 14
- 238000003556 assay Methods 0.000 claims description 13
- FURYAADUZGZUGQ-UHFFFAOYSA-N phenoxybenzene;sulfuric acid Chemical compound OS(O)(=O)=O.C=1C=CC=CC=1OC1=CC=CC=C1 FURYAADUZGZUGQ-UHFFFAOYSA-N 0.000 claims description 13
- 150000008378 aryl ethers Chemical class 0.000 claims description 9
- FDLQZKYLHJJBHD-UHFFFAOYSA-N [3-(aminomethyl)phenyl]methanamine Chemical compound NCC1=CC=CC(CN)=C1 FDLQZKYLHJJBHD-UHFFFAOYSA-N 0.000 claims description 7
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- 108010089254 Cholesterol oxidase Proteins 0.000 abstract 2
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- MFUVDXOKPBAHMC-UHFFFAOYSA-N magnesium;dinitrate;hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O MFUVDXOKPBAHMC-UHFFFAOYSA-N 0.000 description 24
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- 125000003118 aryl group Chemical group 0.000 description 16
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Abstract
Disclosed is a method for determination of the level of cholesterol (RLP-C) in a remnant-like lipoprotein (RLP) contained in a sample in a simple manner and with precision. The method comprises the steps of: (i) reacting a sample with a cholesterol oxidase or with a cholesterol dehydrogenase and an oxidized coenzyme in an aqueous medium containing a surfactant A to remove cholesterol contained in a lipoprotein other than the RLP; (ii) allowing a surfactant B to exist in the reaction solution given after the removal of cholesterol contained in a lipoprotein other than the RLP in step (i), and reacting the resulting mixed solution with a cholesterol ester hydrolase and a cholesterol oxidase or with a cholesterol ester hydrolase, a cholesterol dehydrogenase and an oxidized coenzyme to produce hydrogen peroxide or a reduced coenzyme; and (iii) determining the amount of the hydrogen peroxide or the reduced coenzyme produced in the step (ii).
Description
Technical field
The present invention relates in clinical examination as the method for determination of cholesterol in the remnant-like lipoprotein of the risks and assumptions of arteriosclerosis disease etc., measure with reagent and mensuration test kit.
Background technology
Contain various lipoprotein in the blood.These lipoprotein are categorized as chylomicron (being designated hereinafter simply as CM), vldl (being designated hereinafter simply as VLDL), low-density lipoprotein (being designated hereinafter simply as LDL) and high-density lipoprotein (HDL) (being designated hereinafter simply as HDL) according to the difference of proportion.In addition, there is the density intermediary intermediated-density lipoprotein (being designated hereinafter simply as IDL) that is equivalent to VLDL and LDL in the lipoprotein from VLDL generates to the metabolic process of LDL.The ratio difference of constituents such as cholesterol, tri-glyceride, phosphatide, protein has different effects in vivo in each lipoprotein.
In clinical examination, the cholesterol among the HDL, is frequently measured in the clinical examination field as the positive risks and assumptions of the stearic disease of artery as the negative risks and assumptions of arteriosclerosis, cholesterol among the LDL.
In recent years, the cholesterol in the lipoprotein that generates owing to metabolism of lipid etc. is shown as the closer index of cholesterol that likens to among the LDL of the risks and assumptions of arteriosclerosis.As the lipoprotein that generates owing to metabolism of lipid etc., can enumerate for example remnant-like lipoprotein (being designated hereinafter simply as RLP).
RLP produces in by the process of lipoprotein lipase metabolism and decomposition at lipoprotein, is categorized as residual grain of chyle (being designated hereinafter simply as the residual grain of CM) and the residual grain of vldl (being designated hereinafter simply as the residual grain of VLDL).IDL is included in lipoprotein in the residual grain of VLDL, proportion 1.006～1.019, is called the residual grain of VLDL of narrow sense.
RLP is the abundant lipoprotein of tri-glyceride, and the residual grain of CM contains Apolipoprotein B-48 and apo E as main lipophorin, and the residual grain of VLDL contains Apolipoprotein B-100 and apo E as main lipophorin.
Measuring method as the cholesterol among the RLP (being designated hereinafter simply as RLP-C), the known immunochromatographic method that contains the affinity gel (affinity gel) of anti-apolipoprotein A-1 monoclonal antibody and anti-Apolipoprotein B-100 monoclonal antibody by use separates RLP from serum, and cholesterol contained among this isolating RLP is carried out quantitative methods.The RLP-C quantitative reagent that is applicable to this quantivative approach is by Japanese antibody institute's Co., Ltd.'s sale [goods name: RLP-cholesterol " JIMRO " II] (with reference to non-patent literature 1, non-patent literature 2).About utilizing the RLP-C quantitative method of this immunoabsorption, provide insurance to count by MHLW.But this method is to use the affinity chromatography of antibody, must carry out the lock out operation of sample, so troublesome poeration, measures time-consuming.
On the other hand, RLP-C quantitative method as the lock out operation that does not need sample, known tensio-active agent, rCO or cholesterol dehydrogenase, cholesterol ester hydrolase and the lipid hydrolysis enzyme of making acts on a corpse or other object for laboratory examination and chemical testing, thereby measures the method (with reference to patent documentation 1) of the RLP-C in the corpse or other object for laboratory examination and chemical testing.
Patent documentation 1: TOHKEMY 2001-231597 communique
Non-patent literature 1: arteriosclerosis, 25 (9,10), 371-376 (1998)
Non-patent literature 2:Clinical Chemistry, 48 (2), 217-219 (2002)
Summary of the invention
The object of the present invention is to provide and be used for easy and correctly measure method, reagent and the test kit of the RLP-C of a corpse or other object for laboratory examination and chemical testing.
The present invention relates to following [1]～[27].
[1] method of the cholesterol (being designated hereinafter simply as RLP-C) in a kind of remnant-like lipoprotein of measuring in the corpse or other object for laboratory examination and chemical testing (being designated hereinafter simply as RLP) is characterized in that, carries out following steps (i)～(iii) successively:
(i) containing a corpse or other object for laboratory examination and chemical testing, and be selected from by the polyethylene oxide alkyl ethers phosphoric acid ester, the polyethylene oxide alkyl ethers sulfuric acid, higher alcohol sulfate salt, the HLB value is encircled phenyl ether less than 16.0 polyoxyethylene more, polyoxyethylene encircles the phenyl ether sulfuric acid more, polyoxyethylene polyoxygenated alkene encircles phenyl ether more, quadrol-polyoxyethylene polyoxytrimethylene condenses, the polyoxyethylene alkyl phenyl ether, polyoxyethylene alkyl phenyl ether sulfuric acid, polyoxyethylene short-chain branches alkyl oxide, the molecular weight of polyoxytrimethylene is less than 2750 polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene polyoxygenated alkene short-chain branches alkyl oxide, polyoxyethylene polyoxygenated alkene alkyl oxide, bile acid derivative, the polyoxyethylene Bisphenol A Ether, in the aqueous medium of the tensio-active agent in the group that polyoxyethylene aryl ethers sulfuric acid and polyoxytrimethylene m-xylene diamine are formed (following abbreviate this tensio-active agent as surfactant A), make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps cholesterol dehydrogenase and oxidized coenzyme react, and remove the step of the cholesterol in the RLP lipoprotein in addition;
(ii) make to have removed to exist in the reaction solution after the cholesterol in the lipoprotein beyond the RLP and be selected from by polyoxyethylene polyoxybutylene condenses by above-mentioned steps (i), the HLB value is that the polyoxyethylene more than 16.0 encircles phenyl ether more, polyoxyethylene polyoxygenated alkene long-chain branched-chain alkyl ether, the molecular weight of polyoxytrimethylene be in the group formed of polyoxyethylene polyoxytrimethylene condenses more than 2750 and polyoxyethylene long-chain branched-chain alkyl ether tensio-active agent (below, abbreviate this tensio-active agent as surfactant B), make cholesterol ester hydrolase and rCO, perhaps cholesterol ester hydrolase, cholesterol dehydrogenase and oxidized coenzyme reaction, the step of generation hydrogen peroxide or reduced coenzyme; And
(iii) measure the (ii) middle hydrogen peroxide that generates of above-mentioned steps or the step of reduced coenzyme.
[2] as [1] described method, wherein, step (i) is in the aqueous medium that contains a corpse or other object for laboratory examination and chemical testing and surfactant A, makes the reaction of cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase and a corpse or other object for laboratory examination and chemical testing, removes the step of the cholesterol in the RLP lipoprotein in addition.
[3] as [1] or [2] described method, wherein, being reflected under the polyanion existence of step (i) carried out.
[4] as [3] described method, wherein, surfactant A is to be selected from tensio-active agent in the group of being made up of less than 2750 polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene polyoxygenated alkene short-chain branches alkyl oxide and bile acid derivative the molecular weight of quadrol-polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene alkyl phenyl ether, polyoxyethylene alkyl phenyl ether sulfuric acid, polyoxyethylene short-chain branches alkyl oxide, polyoxytrimethylene [below, abbreviate this tensio-active agent as tensio-active agent (A1)].
[5] as [1] or [2] described method, wherein, the metal ion existence that is reflected at polyanion and divalence of step (i) is carried out down or in the presence of the antibody in being selected from the group of being made up of anti-apolipoprotein B antibody and anti-apo E antibody.
[6] as [5] described method, wherein, surfactant A is for being selected from by the polyethylene oxide alkyl ethers sulfuric acid, polyoxyethylene encircles the phenyl ether sulfuric acid more, polyoxyethylene polyoxygenated alkene encircles phenyl ether more, quadrol-polyoxyethylene polyoxytrimethylene condenses, the polyoxyethylene alkyl phenyl ether, the molecular weight of polyoxytrimethylene is less than 2750 polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene polyoxygenated alkene short-chain branches alkyl oxide, tensio-active agent in the group that polyoxyethylene polyoxygenated alkene alkyl oxide and bile acid derivative are formed [below, abbreviate this tensio-active agent as tensio-active agent (A2)].
[7] as each described method in [1]～[6], wherein, step (i) is carried out under the coexistence of cholesterol ester hydrolase.
[8] as each described method in [1]～[7], wherein, step is (ii) carried out under the coexistence of phosphatide lytic enzyme.
[9] as each described method in [1]～[8], wherein, the elimination of hydrogen peroxide is in the presence of the combination of peroxidase and one of two kinds of oxidative coupling type chromogens that use in oxidative coupling color development reaction or carry out in the presence of catalatic.
[10] a kind of reagent that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it contains cholesterol ester hydrolase, rCO, hydrogen peroxide removes reagent, hydrogen peroxide assay reagents, surfactant A and surfactant B.
[11] a kind of reagent that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it contains cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme, surfactant A and surfactant B.
[12] a kind of reagent that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it contains cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme, reduced coenzyme oxydase, hydrogen peroxide removes reagent, hydrogen peroxide assay reagents, surfactant A and surfactant B.
[13] as [10] or [12] described reagent, wherein, it is to contain peroxidase and the reagent of the combination of one of two kinds of oxidative coupling type chromogens using in oxidative coupling color development reaction or contain catalatic reagent that hydrogen peroxide is removed reagent.
[14] as each described reagent in [10]～[13], it further contains polyanion.
[15] as [14] described reagent, wherein, surfactant A is tensio-active agent (A1).
[16] as each described reagent in [10]～[13], it further contains: contain the reagent of polyanion and divalent-metal ion or contain the reagent that is selected from the antibody in the group of being made up of anti-apolipoprotein B antibody and anti-apo E antibody.
[17] as [16] described reagent, wherein, surfactant A is tensio-active agent (A2).
[18] as each described reagent in [10]～[17], it further contains the phosphatide lytic enzyme.
[19] a kind of test kit that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it is characterized in that, contain first reagent and second reagent, described first reagent contains rCO, hydrogen peroxide is removed reagent and surfactant A, and described second reagent contains hydrogen peroxide assay reagents and surfactant B; And make among any one or two in first reagent and second reagent and contain cholesterol ester hydrolase.
[20] a kind of test kit that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing is characterized in that contain first reagent and second reagent, described first reagent contains cholesterol dehydrogenase, oxidized coenzyme and surfactant A, and described second reagent contains surfactant B; And make among any one or two in first reagent and second reagent and contain cholesterol ester hydrolase.
[21] a kind of test kit that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it is characterized in that, contain first reagent and second reagent, described first reagent contains cholesterol dehydrogenase, oxidized coenzyme, reduced coenzyme oxydase, hydrogen peroxide removes reagent and surfactant A, and described second reagent contains hydrogen peroxide assay reagents and surfactant B; And make among any one or two in first reagent and second reagent and contain cholesterol ester hydrolase.
[22] as [19] or [21] described test kit, wherein, to remove reagent be to contain peroxidase and the reagent of the combination of one of two kinds of oxidative coupling type chromogens using in oxidative coupling color development reaction or contain catalatic reagent to hydrogen peroxide.
[23] as each described test kit in [19]～[22], wherein, first reagent further contains polyanion.
[24] as [23] described test kit, wherein, surfactant A is tensio-active agent (A1).
[25] as each described test kit in [19]～[22], wherein, first reagent further contains the metal ion of polyanion and divalence or is selected from antibody in the group of being made up of anti-apolipoprotein B antibody and anti-apo E antibody.
[26] as [25] described test kit, wherein, surfactant A is tensio-active agent (A2).
[27] as each described test kit in [19]～[26], wherein, second reagent further contains the phosphatide lytic enzyme.
The invention effect
By the present invention, can provide the lock out operation that need not a corpse or other object for laboratory examination and chemical testing, easy and correctly measure method, reagent and the test kit of RLP-C.
Description of drawings
Fig. 1 is the correlogram of mensuration with the mensuration of using RLP-cholesterol " JIMRO " II (Japanese antibody institute corporate system) of the expression test kit that uses reference example 1.
Embodiment
Among the present invention, so-called RLP, be meant the lipoprotein of residual grain of VLDL and the residual grain combination of CM, RLP-C is meant free type cholesterol and the cholesterol of ester type cholesterol combination, the i.e. free type cholesterol in free type cholesterol in the residual grain of VLDL and ester type cholesterol and the residual grain of CM and the cholesterol of ester type cholesterol combination among the RLP.
RLP-C measuring method of the present invention has following feature: do not need the lock out operation of a corpse or other object for laboratory examination and chemical testing, in addition, before measuring RLP-C, have the step of removing the cholesterol in the RLP lipoprotein in addition.At this, " lipoprotein beyond the RLP " in " removing the cholesterol in the RLP lipoprotein in addition " is meant any lipoprotein of the lipoprotein beyond the RLP, not only represent RLP whole lipoprotein in addition, and one or more lipoprotein beyond the expression RLP.In addition, " cholesterol " in " removing the cholesterol in the lipoprotein beyond the RLP " not only represented the combination of whole free type cholesterol and whole ester type cholesterol, and represents all or part of free type cholesterol, all or part of ester type cholesterol, all combination of free type cholesterol and the free type cholesterol of combination, a part of a part of ester type cholesterol and whole ester type cholesterol and part combinations of type cholesterol and a part of ester type cholesterol that dissociates.
A corpse or other object for laboratory examination and chemical testing that uses in the measuring method of the present invention for example can be enumerated: whole blood, blood plasma, serum, spinal fluid, saliva, amniotic fluid, urine, sweat, pancreatic juice etc., preferred blood plasma and serum.
Cholesterol ester hydrolase among the present invention, get final product so long as have the enzyme of hydrolysis cholesteryl ester ability, be not particularly limited, for example, except can using animal, plant or microbe-derived Sterol esterase, lipoprotein lipase, also can use the Sterol esterase made by the genetic engineering method, lipoprotein lipase etc.
Cholesterol ester hydrolase can use literalness cholesterol ester hydrolase, also can use the cholesterol ester hydrolase after the chemically modified.In addition, cholesterol ester hydrolase also can use commercially available product.
As commercially available cholesterol ester hydrolase, can enumerate: Sterol esterase " Amano " 2 (CHE2; The amano enzyme corporate system), Sterol esterase " Amano " 3 (CHE3; The amano enzyme corporate system), lipoprotein lipase " Amano " 6 (LPL6; The amano enzyme corporate system), lipoprotein lipase (LPL-311; Japan's textile company system), lipoprotein lipase (LPL-312; Japan's textile company system), Sterol esterase (COE-301; Japan's textile company system), lipoprotein lipase (LPBP; Asahi Chemical Industry's corporate system), Sterol esterase (CEBP-M; Asahi Chemical Industry's corporate system), Sterol esterase (CEN; Asahi Chemical Industry's corporate system) etc.In addition, in the present invention, also the cholesterol ester hydrolase more than 2 kinds can be used in combination.
In the chemically modified of cholesterol ester hydrolase, as the group that is used to modify this enzyme (chemically modified group), for example can enumerate: being the group of principal constituent with the polyoxyethylene glycol, being the group of principal constituent, the group with multipolymer of polypropylene glycol and polyoxyethylene glycol, the group that contains water-soluble polysaccharides, sulfo group propyl group, sulfo group butyl, polyurethane-base with the polypropylene glycol, having the group of chelating function etc., preferably is the group of principal constituent with the polyoxyethylene glycol.As water-soluble polysaccharides, for example can enumerate: dextran, pulullan polysaccharide, Zulkovsky starch etc.
As the reagent that is used for the chemically modified cholesterol ester hydrolase (chemical modifier), can enumerate have simultaneously above-mentioned chemically modified group and can with the functional group of reactions such as the amino of enzyme, carboxyl, sulfydryl or the compound of structure etc.As functional group or the structure that can react with the amino in the enzyme, for example can enumerate: carboxyl, active ester groups (N-hydroxy-succinamide base etc.), acid anhydrides, acyl chlorides, aldehyde, epoxy group(ing), 1,3-N-morpholinopropanesulfonic acid lactone, 1,4-butyl sultone etc.As can with the functional group or the structure of carboxyl reaction in the enzyme, can enumerate for example amino etc.As functional group or the structure that can react with the sulfydryl in the enzyme, for example can enumerate: dimaleoyl imino, disulphide, alpha-halogen ester (alpha-iodine is for ester) etc.
As chemical modifier, also can use commercially available product.As commercially available chemical modifier, can enumerate: having with the polyoxyethylene glycol is the group of principal constituent and the サ Application Block ラ イ ト VFM-4101 of N-hydroxy-succinamide group, サ Application Block ラ イ ト ME-050AS, サ Application Block ラ イ ト DE-030AS (making) by Nof Corp., have with the polyalkylene glycol be the group of principal constituent and acid anhydride structure サ Application Block ラ イ ト AKM series (for example, サ Application Block ラ イ ト AKM-1510 etc.), サ Application Block ラ イ ト ADM series, サ Application Block ラ イ ト ACM series (making) by Nof Corp., have with the polyoxyethylene glycol is the group of principal constituent and the EPOX-3400 of epoxy group(ing), M-EPOX-5000 (making) by Sheawater Polymers company, comprise the group with chelating function and the Diethylenetriaminee (DETA)-N of acid anhydride structure, N, N '; N ", N "-pentaacetic acid acid anhydride (DTPAanhydride: colleague's chemistry institute company makes) etc.
The chemically modified of cholesterol ester hydrolase for example can be carried out by the following method, but is not limited to this method.At first, cholesterol ester hydrolase is dissolved in the damping fluid (for example HEPES damping fluid) more than the pH8.0,, and stirred 5 minutes～5 hours at 0～55 ℃ of chemical modifier that adds 0.01～500 times of molar weight down.In the enzyme reaction of reality, as cholesterol ester hydrolase, not only can use this reaction solution itself, and can use as required by ultra-filtration membrane etc. and remove liquid behind the unreacted chemical modifier through chemically modified.
The concentration of the cholesterol ester hydrolase that uses in the method for this reaction, get final product so long as can carry out the concentration of RLP-C mensuration of the present invention, be not particularly limited, preferably the concentration of 0.001～2000U/mL, the more preferably concentration of 0.005～1000U/mL in reaction solution.
As rCO of the present invention, then be not particularly limited so long as have the enzyme of cholesterol oxide generation hydrogen peroxide ability, for example: except can using animal, plant, microbe-derived rCO, also can use the rCO of making by genetic engineering method, also can use commercially available product, for example rCO " Amano " 1 (CHOD1; The manufacturing of amano enzyme company), rCO (CHOPE; The manufacturing of キ Star コ one マ Application company), rCO (CHO-CE; The manufacturing of キ Star コ one マ Application company), rCO (COO321; Japan's textile company is made) etc.In addition, among the present invention, also the rCO more than 2 kinds can be used in combination.
RCO both can unmodified enzyme, also can be the enzyme that has carried out chemically modified.The rCO of chemically modified can be by for example: use aforesaid chemical modifier, by aforesaid chemical modification method manufacturing.
The concentration of the rCO that uses in the method for this reaction then is not particularly limited so long as can carry out the concentration of RLP-C mensuration of the present invention, preferably the concentration of 0.001～2000U/mL in reaction solution, the more preferably concentration of 0.005～1000U/mL.
As the cholesterol dehydrogenase among the present invention, then be not particularly limited so long as have the enzyme that in the presence of the oxydase coenzyme, the cholesterol oxidation is generated the reduced coenzyme ability, for example, except can using animal, plant or microbe-derived cholesterol dehydrogenase, also can use the cholesterol dehydrogenase of making by the genetic engineering method.Also can use cholesterol dehydrogenase " Amano " 5 (CHDH5; Amano enzyme company makes) etc. commercially available product.In addition, among the present invention, also cholesterol dehydrogenase more than 2 kinds can be used in combination.Cholesterol dehydrogenase can be literalness enzyme, also can be the enzyme through chemically modified.Cholesterol dehydrogenase through chemically modified for example can use aforesaid chemical modifier by aforesaid chemical modification method manufacturing.
The concentration of the cholesterol dehydrogenase that uses in the method for this reaction then is not particularly limited so long as can carry out the concentration of RLP-C mensuration of the present invention, preferably the concentration of 0.001～2000U/mL in reaction solution, the more preferably concentration of 0.005～1000U/mL.
As the oxidized coenzyme that uses in the mensuration of using cholesterol dehydrogenase, for example can enumerate: NAD (P)
+, thio-NAD (P)
+Deng.As the reduced coenzyme that the reaction by cholesterol dehydrogenase generates, for example can enumerate: NAD (P) H, thio-NAD (P) H etc.
The concentration of the oxidized coenzyme that uses in the method for the present invention then is not particularly limited so long as can carry out the concentration of RLP-C mensuration of the present invention, preferably the concentration of 0.01～400mmol/L in reaction solution, the more preferably concentration of 0.1～100mmol/L.
As reduced coenzyme oxydase of the present invention, then be not particularly limited so long as have the enzyme of the reduced coenzyme generation hydrogen peroxide ability that generates by reaction by cholesterol dehydrogenase, for example can enumerate: NAD (P) H oxydase etc.The reduced coenzyme oxydase also can use commercially available product.As commercially available reduced coenzyme oxydase, can enumerate NADH Oxidase (manufacturing of CosmoBio company).
The oxidasic concentration of the reduced coenzyme that uses in the method for the present invention then is not particularly limited so long as can carry out the concentration of RLP-C mensuration of the present invention, preferably the concentration of 0.01～400U/mL in reaction solution, the more preferably concentration of 0.02～200U/mL.
As phosphatide lytic enzyme in of the present invention, then be not particularly limited so long as have the enzyme that decomposes the phosphatide ability, for example can enumerate: animal, plant or microbe-derived Phospholipase D, Phospholipase C, Phospholipase A2, lysophospholipase etc., wherein, preferred Phospholipase D or Phospholipase A2.
As the phosphatide lytic enzyme, also can use commercially available product.As commercially available phosphatide lytic enzyme, can enumerate Phospholipase D (PLD; Asahi Chemical Industry's corporate system), Phospholipase D (PLDP; Asahi Chemical Industry's corporate system), Phospholipase C (PLC; Asahi Chemical Industry's corporate system), Phospholipase A2 (PLA2; Asahi Chemical Industry's corporate system) etc.
The concentration of the phosphatide lytic enzyme that uses in the method for the present invention then is not particularly limited so long as can carry out the concentration of RLP-C mensuration of the present invention, preferably the concentration of 0.01～200U/mL in reaction solution, the more preferably concentration of 0.1～100U/mL.
As polyanion of the present invention, then be not particularly limited so long as can carry out the polyanion of RLP-C mensuration of the present invention, for example can enumerate: T 500 or its salt, heparin or its salt, phospho-wolframic acid or its salt, sulfated cyclodextrin or its salt, sulfated oligosaccharide or its salt, polycarboxylic acid's type polymkeric substance etc.As sulfated oligosaccharide, for example can enumerate: sulfation agarose, sulfation trehalose, chondroitin sulfate etc.Salt for example can be enumerated: sodium salt, sylvite, lithium salts, ammonium salt, magnesium salts etc.In addition, among the present invention, can use the polyanion more than 2 kinds.Polyanion concentration in measuring as RLP-C of the present invention then is not particularly limited so long as can carry out the concentration that RLP-C of the present invention measures, and the concentration in the preferred reaction liquid is 0.0001～10%, and more preferably 0.001～1%.
As the divalent-metal ion among the present invention, then be not particularly limited so long as can carry out the divalent-metal ion of RLP-C mensuration of the present invention, for example can enumerate: cupric ion, iron ion, mn ion and magnesium ion etc.In addition, among the present invention, can use the divalent-metal ion more than 2 kinds.The concentration of the divalent-metal ion in measuring as RLP-C of the present invention then is not particularly limited so long as can carry out the concentration that RLP-C of the present invention measures, and the concentration in the preferred reaction liquid is 0.0001～10%, and more preferably 0.001～1%.
As the anti-apolipoprotein B antibody among the present invention, then be not particularly limited so long as can carry out the anti-apolipoprotein B antibody of RLP-C mensuration of the present invention, can enumerate with the lipoprotein that contains Apolipoprotein B-100 (for example, the residual grain of LDL, VLDL) bonded antibody (anti-Apolipoprotein B-100 antibody), with the lipoprotein that contains Apolipoprotein B-48 (for example, the residual grain of CM) bonded antibody (anti-Apolipoprotein B-48 antibody) etc.Anti-apolipoprotein B antibody also can use commercially available product.As the commercially available product of anti-apolipoprotein B, for example can enumerate: Antiapoliproprotein B antibody, Anti Apolipoprotein B100 antibody (above is that CosmoBio company makes) etc.
As the anti-apo E antibody among the present invention, then be not particularly limited so long as can carry out the anti-apo E antibody of RLP-C mensuration of the present invention, can enumerate and contain lipoprotein (for example, the residual grain of chyle, the residual grain of VLDL) the bonded antibody of apo E etc.Anti-apo E antibody also can use commercially available product.As the commercially available product of anti-apo E antibody, for example can enumerate: Anti apolipoprotein E antibody (manufacturing of CosmoBio company) etc.
As surfactant A of the present invention, can enumerate: polyethylene oxide alkyl ethers phosphoric acid ester (being designated hereinafter simply as the POE alkyl ether phosphate), polyethylene oxide alkyl ethers sulfuric acid (being designated hereinafter simply as the POE alkyl ether sulphate salts), higher alcohol sulfate salt, the HLB value is encircled phenyl ether (be designated hereinafter simply as the HLB value and encircle phenyl ether less than 16.0 POE more) less than 16.0 polyoxyethylene more, polyoxyethylene encircles phenyl ether sulfuric acid (be designated hereinafter simply as POE and encircle the phenyl ether sulfuric acid more) more, polyoxyethylene polyoxygenated alkene encircles phenyl ether (be designated hereinafter simply as POEPOA and encircle phenyl ether more) more, quadrol-polyoxyethylene polyoxytrimethylene condenses (being designated hereinafter simply as quadrol-POEPOP condenses), polyoxyethylene alkyl phenyl ether (being designated hereinafter simply as the POE alkyl phenyl ether), polyoxyethylene alkyl phenyl ether sulfuric acid (being designated hereinafter simply as POE alkyl phenyl ether sulfuric acid), polyoxyethylene short-chain branches alkyl oxide (being designated hereinafter simply as POE short-chain branches alkyl oxide), the molecular weight of polyoxytrimethylene is less than 2750 polyoxyethylene polyoxytrimethylene condenses (molecular weight that is designated hereinafter simply as POP less than 2750 POEPOP condenses), polyoxyethylene polyoxygenated alkene short-chain branches alkyl oxide (being designated hereinafter simply as POEPOA short-chain branches alkyl oxide), polyoxyethylene polyoxygenated alkene alkyl oxide (being designated hereinafter simply as the POEPOA alkyl oxide), bile acid derivative, polyoxyethylene Bisphenol A Ether (being designated hereinafter simply as the POE Bisphenol A Ether), polyoxyethylene aryl ethers sulfuric acid (being designated hereinafter simply as POE aryl ethers sulfuric acid), and polyoxytrimethylene m-xylene diamine (POP m-xylene diamine) etc.
As the alkyl in the POE alkyl ether phosphate, can enumerate carbonatoms 1～30, methyl for example, ethyl, propyl group, butyl, amyl group, hexyl, heptyl, octyl group, nonyl, decyl, undecyl, dodecyl (lauryl), tridecyl, tetradecyl (myristyl), pentadecyl, hexadecyl (hexadecyl), heptadecyl, octadecyl (stearyl), nonadecyl, eicosyl, heneicosyl, docosyl (Shan Yu base), tricosyl, tetracosyl, pentacosyl, ceryl, heptacosyl, octacosyl, nonacosyl, triacontyl etc.The concrete example of POE alkyl ether phosphate (commercially available product) can be enumerated プ ラ イ サ one Off A208D, プ ラ イ サ one Off A210G, プ ラ イ サ one Off A215C, プ ラ イ サ one Off A217E, プ ラ イ サ one Off A219B (more than be that the first industrial drugmaker makes) etc.
As the alkyl in the POE alkyl ether sulphate salts, can enumerate carbonatoms 1～30, methyl for example, ethyl, propyl group, butyl, amyl group, hexyl, heptyl, octyl group, nonyl, decyl, undecyl, dodecyl (lauryl), tridecyl, tetradecyl (myristyl), pentadecyl, hexadecyl (hexadecyl), heptadecyl, octadecyl (stearyl), nonadecyl, eicosyl, heneicosyl, docosyl (Shan Yu base), tricosyl, tetracosyl, pentacosyl, ceryl, heptacosyl, octacosyl, nonacosyl, triacontyl etc.As the commercially available product of POE alkyl ether sulphate salts, can enumerate ニ ユ one コ one Le 1105-SN, ニ ユ one コ one Le 1305-SN, ニ ユ one コ one Le 1703-SFD (above make), パ one ソ Off ト EF, パ one ソ Off ト EP, パ one ソ Off ト EL, パ one ソ Off ト EK, パ one ソ Off ト EFT, パ one ソ Off ト EDO (above make) etc. by Nof Corp. by Japanese emulsifying agent company.As the commercially available product of higher alcohol sulfate salt, can enumerate モ ノ ゲ Application, モ ノ ゲ Application 170T, モ ノ ゲ Application LH, モ ノ ゲ Application T-423, モ ノ ゲ Application Y-100, モ ノ ゲ Application Y-500 (above) etc. by the manufacturing of the first industrial drugmaker.
The HLB value is encircled many rings phenyl in the phenyl ether less than 16.0 POE more, the phenyl that can enumerate the phenyl that replaced by the group (substituting group) that has 1 aromatic ring in 2 above groups, is replaced by the group (substituting group) that has 2 above aromatic rings in one or more groups etc.As the group that has an aromatic ring in the group, for example can enumerate: benzyl, 1-(phenyl) ethyl etc.As the group that has 2 above aromatic rings in the group, can enumerate for example naphthyl etc.
Encircle the commercially available product of phenyl ether as the HLB value less than 16.0 POE more, can enumerate エ マ Le ゲ Application A-60, エ マ Le ゲ Application A-90, エ マ Le ゲ Application B-66 (above make), BLAUNON DSP-9, BLAUNON DSP-12.5, BLAUNON TSP-5, BLAUNON TSP-7.5, BLAUNON TSP-16 (above make), ニ ユ one コ one Le 610, ニ ユ one コ one Le 707, ニ ユ one コ one Le 710, ニ ユ one コ one Le 2609, ニ ユ one コ one Le 2614 (above make) etc. by Japanese emulsifying agent company by blue or green wood oil fat company by KAO. Corp. SA.
POE encircles the many rings phenyl in the phenyl ether thioesters salt more, the phenyl that can enumerate the phenyl that replaced by the group (substituting group) that has 1 aromatic ring in 2 above groups, is replaced by the group (substituting group) that has 2 above aromatic rings in one or more groups etc.As the group that has an aromatic ring in the group, for example can enumerate: benzyl, 1-(phenyl) ethyl etc.As the group that has 2 above aromatic rings in the group, can enumerate for example naphthyl etc.Encircle the commercially available product of phenyl ether sulfuric acid as POE more, can enumerate ニ ユ one コ one Le 707-SF, ニ ユ one コ one Le 707-SFC, ニ ユ one コ one Le 707-SN, ニ ユ one コ one Le 723-SF, ニ ユ one コ one Le 740-SF (above make) etc. by Japanese emulsifying agent company.
POEPOA encircles the many rings phenyl in the phenyl ether more, the phenyl that can enumerate the phenyl that replaced by the group (substituting group) that has 1 aromatic ring in 2 above groups, is replaced by the group (substituting group) that has 2 above aromatic rings in one or more groups etc.As the group that has an aromatic ring in the group, for example can enumerate: benzyl, 1-(phenyl) ethyl etc.Encircle polyoxygenated alkene in phenyl ether as POEPOA more, can enumerate for example polyoxytrimethylene beyond the polyoxyethylene, polyoxygenated class D etc.Encircle the commercially available product of phenyl ether as POEPOA more, can enumerate ニ ユ one カ Le ゲ Application CP-120, ニ ユ one カ Le ゲ Application GP-120 (above make), ニ ユ one コ one Le 707-F, ニ ユ one コ one Le 710-F, ニ ユ one コ one Le 714-F, ニ ユ one コ one Le 2608-F, ニ ユ one コ one Le 2616-F, ニ ユ one コ one Le 2618-F (above make) etc. by Japanese emulsifying agent company by this grease of bamboo company.
As the commercially available product of quadrol-POEPOP condenses, can enumerate プ Le ロ ニ Star Network TR-701, プ Le ロ ニ Star Network TR-702, プ Le ロ ニ Star Network TR-704, プ Le ロ ニ Star Network TR-913R (above), エ チ レ Application ジ ア ミ Application PO40EO40, quadrol PO52EO60, ユ ニ Le one Block (above) etc. by Nof Corp.'s manufacturing by the manufacturing of rising sun electrification company.
As the alkyl of POE alkyl phenyl ether, can enumerate nonyl, octyl group etc.As the commercially available product of POE alkyl phenyl ether, can enumerate ト リ ト Application N57, ト リ ト Application N60, ト リ ト Application X100, ト リ ト Application X-102, ト リ ト Application X-114, ト リ ト Application X35 (above) by the manufacturing of SIGMA company, ニ Star コ one Le NP-10, ニ Star コ one Le NP 1, ニ Star コ one Le NP-18TX, ニ Star コ one Le NP-20, ニ Star コ one Le NP-7.5, ニ Star コ one Le OP-10, ニ Star コ one Le OP-30 (above) by day Optical Chemical Company's manufacturing, エ マ Le ゲ Application 810, エ マ Le ゲ Application 903, エ マ Le ゲ Application 905, エ マ Le ゲ Application 906, エ マ Le ゲ Application 909, エ マ Le ゲ Application 910, エ マ Le ゲ Application 911, エ マ Le ゲ Application 913, エ マ Le ゲ Application 920, エ マ Le ゲ Application 930, エ マ Le ゲ Application 931, エ マ Le ゲ Application 950 (above) by KAO. Corp. SA's manufacturing, ノ ニ オ Application HS-204.5, ノ ニ オ Application HS-206, ノ ニ オ Application HS-208, ノ ニ オ Application HS-210, ノ ニ オ Application HS-215, ノ ニ オ Application HS-220, ノ ニ オ Application HS-240, ノ ニ オ Application NS-204.5, ノ ニ オ Application NS-206, ノ ニ オ Application NS-208.5, ノ ニ オ Application NS-210, ノ ニ オ Application NS-212, ノ ニ オ Application NS-215, ノ ニ オ Application NS-220, ノ ニ オ Application NS-230, ノ ニ オ Application NS-240, ノ ニ オ Application NS-270 (above) by Nof Corp.'s manufacturing, モ リ ノ one Le NP-40, モ リ ノ one Le NP-60, モ リ ノ one Le NP-85, モ リ ノ one Le NP-100, モ リ ノ one Le NP-120, モ リ ノ one Le NP-160, モ リ ノ one Le NP-300, モ リ ノ one Le NP-400, モ リ one Le NP-600, モ リ ノ one Le NP-800 (above) by the manufacturing of モ リ ノ one Le chemical company, オ Network Port one Le 50, オ Network Port one Le 60, オ Network Port one Le 80, オ Network Port one Le 100 (above make) etc. by the Sanyo company that changes into.
As the alkyl in the POE alkyl phenyl ether sulfuric acid, can enumerate carbonatoms 1～30, methyl for example, ethyl, propyl group, butyl, amyl group, hexyl, heptyl, octyl group, nonyl, decyl, undecyl, dodecyl (lauryl), tridecyl, tetradecyl (myristyl), pentadecyl, hexadecyl (hexadecyl), heptadecyl, octadecyl (stearyl), nonadecyl, eicosyl, heneicosyl, docosyl (Shan Yu base), tricosyl, tetracosyl, pentacosyl, ceryl, heptacosyl, octacosyl, nonacosyl, triacontyl etc.As the commercially available product of POE alkyl phenyl ether sulfuric acid, can enumerate Ha イ テ ノ one Le N-07, Ha イ テ ノ one Le N-08, Ha イ テ ノ one Le N-17 (above) etc. by the manufacturing of the first industrial drugmaker.
As the short-chain branches alkyl in the POE short-chain branches alkyl oxide, can enumerate for example isohexyl of carbonatoms 6～12, different heptyl, iso-octyl, different nonyl, isodecyl, different undecyl, Permethyl 99A. base etc.As the commercially available product of POE short-chain branches alkyl oxide, can enumerate ノ イ ゲ Application SD110, ノ イ ゲ Application SD150, ノ イ ゲ Application SD300, ノ イ ゲ Application SD400 (above) etc. by the manufacturing of the first industrial drugmaker.
As the molecular weight of POP commercially available product, can enumerate プ Le ロ ニ Star Network 25R-1 less than 2750 POEPOP condenses, プ Le ロ ニ Star Network 25R-2, プ Le ロ ニ Star Network 17R-1, プ Le ロ ニ Star Network 17R-2, プ Le ロ ニ Star Network 17R-3, プ Le ロ ニ Star Network L-33, プ Le ロ ニ Star Network L-34, プ Le ロ ニ Star Network L-62, プ Le ロ ニ Star Network L-64, プ Le ロ ニ Star Network P-65, プ Le ロ ニ Star Network F-68, プ Le ロ ニ Star Network P-84, プ Le ロ ニ Star Network P-85, プ Le ロ ニ Star Network F-88 (above) by the manufacturing of rising sun electrification company, エ パ Application 420, エ パ Application 720, エ パ Application U103, エ パ Application U105, エ パ Application U108 (above) by the manufacturing of the first industrial drugmaker, モ リ ノ one Le PP-100, モ リ ノ one Le PP-300, モ リ ノ one Le PP-400 (above) by the manufacturing of モ リ ノ one Le chemical company, BLAUNON P-101, BLAUNONP-106, BLAUNON P-171, BLAUNON P-172, BLAUNON P-174, BLAUNON P-201, BLAUNON EP-0840, BLAUNON EP-0480, BLAUNON EP-0670, BLAUNON EP-1461 (above make) etc. by blue or green wood oil fat company.
As the polyoxygenated alkene in the POEPOA short-chain branches alkyl oxide, can enumerate polyoxyethylene for example polyoxytrimethylene, polyoxybutylene etc. in addition.As the short-chain branches alkyl in the POEPOA short-chain branches alkyl oxide, can enumerate for example isohexyl of carbonatoms 6～19, different heptyl, iso-octyl, different nonyl, isodecyl, different undecyl, Permethyl 99A. base, isotridecyl, different tetradecyl, different pentadecyl, isocetyl, hexyl decyl, different heptadecyl, isooctadecane base, norphytane base etc.As the commercially available product of POEPOA short-chain branches alkyl oxide, can enumerate Off ア イ Application サ one Block IDEP-604, Off ア イ Application サ one Block IDEP-608, Off ア イ Application サ one Block IDEP-802, ワ Application グ one サ one Off ID-50, ワ Application グ one サ one Off ID-70, ワ Application ダ one サ one Off ID-90 (above make), ノ イ ゲ Application XL-140, ノ イ ゲ Application XL-160, ノ イ ゲ Application XL-400, ノ イ ゲ Application XL-1000F (above make) etc. by the first industrial drugmaker by blue or green wood oil fat company.
As the polyoxygenated alkene in the POEPOA alkyl oxide, can enumerate polyoxyethylene for example polyoxytrimethylene, polyoxybutylene etc. in addition.As the alkyl in the POEPOA alkyl oxide, can enumerate carbonatoms 1～30, methyl for example, ethyl, propyl group, butyl, amyl group, hexyl, heptyl, octyl group, nonyl, decyl, undecyl, dodecyl (lauryl), tridecyl, tetradecyl (myristyl), pentadecyl, hexadecyl (hexadecyl), heptadecyl, octadecyl (stearyl), nonadecyl, eicosyl, heneicosyl, docosyl (Shan Yu base), tricosyl, tetracosyl, pentacosyl, ceryl, heptacosyl, octacosyl, nonacosyl, triacontyl etc.As the commercially available product of POEPOA alkyl oxide, can enumerate EMALEX DAPE-207, EMALEX DAPE-210, EMALEX DAPE-212, EMALEX DAPE-215, EMALEX DAPE-220, EMALEX DAPE-230 (above) by the manufacturing of nihon emulsion company, エ マ Le ゲ Application LS-110, エ マ Le ゲ Application MS-110 (above) by KAO. Corp. SA's manufacturing, ワ Application グ one サ one Off RL-80, ワ Application グ one サ one Off RL-100, ワ Application グ one サ one Off RL-140, ワ Application グ one サ one Off S-800, ワ Application グ one サ one Off S-1000, ワ Application グ one サ one Off S-1400 (above make) by blue or green wood oil fat company, ユ ニ セ one Off PKA-5015, ユ ニ セ one Off PKA-5016, ユ ニ セ one Off PKA-5017, ユ ニ セ one Off LM-2 (above) etc. by Nof Corp.'s manufacturing.
As bile acid derivative, for example can enumerate cholic acid or its salt, taurocholate or its salt, glycocholic acid or its salt, lithocholic acid or its salt, Septochol or its salt, gallodesoxycholic acid or its salt, Ursodeoxycholic Acid (UDCA) or its salt, 7-oxo lithocholic acid or its salt, 12-oxo lithocholic acid or its salt, 12-oxo gallodesoxycholic acid or its salt, 7-oxo Septochol or its salt, Iocholic acid or its salt, hyodeoxycholic acid or its salt, Felacrinos or its salt, CHAPS{3-[(3-courage amido propyl group) dimethylammonio] propanesulfonic acid; Colleague's chemistry institute company makes }, CHAPSO{3-[(3-courage amido propyl group) dimethylammonio]-the 2-hydroxy-propanesulfonic acid; Colleague's chemistry institute company makes }, BIGCHAP[N, two (3-D-glucose amido propyl group) the courage acid amides of N-; Colleague's chemistry institute company makes], deoxidation BIGCHAP[N, two (3-D-glucose amido propyl group) the deoxidation courage acid amides of N-; Colleague's chemistry institute company makes] etc.Salt can be enumerated for example ammonium salt, lithium salts, sodium salt, sylvite, magnesium salts, calcium salt etc.
As the commercially available product of POE Bisphenol A Ether, can enumerate BLAUNON BEO-6, BLAUNON BEO-10, BLAUNON BEO-17.5 (above make) etc. by blue or green wood oil fat company.
Aryl in the operation POE aryl ethers sulfuric acid can be enumerated phenyl, naphthyl etc.As the commercially available product of POE aryl ethers sulfuric acid, can enumerate ニ ユ one コ one Le B4-SN, ニ ユ one コ one Le B13-SN (above make) etc. by Japanese emulsifying agent company.
As the commercially available product of POP m-xylene diamine, can enumerate BLAUNONMXDA-PO1, BLAUNON MXDA-PO2, BLAUNON MXDA-PO4 (above make) etc. by blue or green wood oil fat company.
Among the present invention, surfactant A, promptly be selected from alkyl ether phosphate by POE, the POE alkyl ether sulphate salts, higher alcohol sulfate salt, the HLB value is encircled phenyl ether less than 16.0 POE more, POE encircles the phenyl ether sulfuric acid more, POEPOA encircles phenyl ether more, quadrol-POEPOP condenses, the POE alkyl phenyl ether, POE alkyl phenyl ether sulfuric acid, POE short-chain branches alkyl oxide, the molecular weight of POP is less than 2750 POEPOP condenses, POEPOA short-chain branches base ether, the POEPOA alkyl oxide, bile acid derivative, the POE Bisphenol A Ether, POE aryl ethers sulfuric acid, and the tensio-active agent in the group of POP m-xylene diamine composition, can be independent, promptly at polyanion, the combination of polyanion and divalent-metal ion, use under anti-apolipoprotein B antibody and the non-existent situation of anti-apo E antibody.
Among the present invention, tensio-active agent (A1) is to be selected from surfactant A, can be the group of the tensio-active agent that uses in the presence of or not the polyanion and at the combination of polyanion and divalent-metal ion or anti-apolipoprotein B antibody and anti-apo E antibody.As tensio-active agent (A1), for example can enumerate: the molecular weight of quadrol-POEPOP condenses, POE alkyl phenyl ether, POE alkyl phenyl ether sulfuric acid, POE short-chain branches alkyl oxide, POP is less than 2750 POEPOP condenses, POEPOA short-chain branches alkyl oxide and bile acid derivative.
Among the present invention, tensio-active agent (A2) is selected from surfactant A, polyanion and divalent-metal ion or be selected from anti-apolipoprotein B antibody and group that anti-apo E antibody is formed in antibody in the presence of the tensio-active agent group that uses, also contain some tensio-active agent (A1).As tensio-active agent (A2), can enumerate for example POE alkyl ether sulphate salts, POE and encircle molecular weight that phenyl ether sulfuric acid, POEPOA encircle phenyl ether, quadrol-POEPOP condenses, POE alkyl phenyl ether, POP more more less than 2750 POEPOP condenses, POEPOA short-chain branches alkyl oxide, POEPOA alkyl oxide and bile acid derivative.
Tensio-active agent (A1) is the tensio-active agent group who is selected from surfactant A as previously mentioned, for example use according to the reagent of embodiment 2 record form preparation reagent, be in the main composition composition during the reagent of embodiment 2 records is formed, (for example add polyanion, dextran sulfate sodium), and add the reagent that other various tensio-active agents replace エ マ Le ゲ Application B-66 preparation, method by embodiments of the invention 77 or 78 is studied, and can suitably select thus.
Tensio-active agent (A2) is the tensio-active agent group who is selected from surfactant A as previously mentioned, for example use the reagent of forming preparation according to the reagent of embodiment 2 records, be in the main composition composition during the reagent of embodiment 2 record is formed, the combination of adding polyanion and divalent-metal ion (for example, the combination of dextran sulfate sodium and magnesium nitrate), the reagent that perhaps is selected from the antibody in the group that anti-apolipoprotein B antibody and anti-apo E antibody forms and adds other various tensio-active agents preparations replaces エ マ Le ゲ Application B-66, method according to embodiments of the invention 77 or 78 is studied, and can suitably select.
Among the present invention, surfactant A, tensio-active agent (A1) and tensio-active agent (A2) can will be selected from a kind of of tensio-active agent group separately or be used in combination more than 2 kinds.
The concentration of the surfactant A in measuring as RLP-C of the present invention is measured and then is not particularly limited as long as can carry out RLP-C of the present invention, and preferably the concentration in reaction solution is 0.0001～5%, and more preferably 0.0005～2%.
As the surfactant B among the present invention, can enumerate polyoxyethylene polyoxybutylene condenses (being designated hereinafter simply as the POEPOB condenses), the HLB value is that the polyoxygenated more than 16.0 is encircled phenyl ether (POE that is designated hereinafter simply as the HLB value and is more than 16.0 encircles phenyl ether more) more, polyoxyethylene polyoxygenated alkene long-chain branched-chain alkyl ether (being designated hereinafter simply as POEPOA long-chain branched-chain alkyl ether), the molecular weight of polyoxytrimethylene is the polyoxyethylene polyoxytrimethylene condenses (molecular weight that is designated hereinafter simply as POP is the POEPOP condenses more than 2750) more than 2750, and polyoxyethylene long-chain branched-chain alkyl ether (being designated hereinafter simply as POE long-chain branched-chain alkyl ether) etc.Surfactant B is in the reaction solution after the cholesterol of adding in the lipoprotein of removing beyond the RLP, can make the cholesterol among the RLP and the tensio-active agent of enzyme reaction.
As the molecular weight of the part of the polyoxybutylene in the POEPOB condenses, preferred more than 700, more preferably more than 1000.As the commercially available product of POEPOB condenses, can enumerate プ ロ ノ Application B-204, プ ロ ノ Application B-208 (above) etc. by Nof Corp.'s manufacturing.
The HLB value is that the POE more than 16.0 encircles the many rings phenyl in the phenyl ether more, the phenyl that can enumerate the phenyl that replaced by the group (substituting group) that has 1 aromatic ring in 2 above groups, is replaced by the group (substituting group) that has 2 above aromatic rings in one or more groups etc.As the group that has an aromatic ring in the group, for example can enumerate: benzyl, 1-(phenyl) ethyl etc.As the group that has 2 above aromatic rings in the group, can enumerate for example naphthyl etc.As the HLB value is the commercially available product that POE more than 16.0 encircles phenyl ether more, can enumerate BLAUNON TSP-50 (blue or green wood oil fat company make), エ マ Le ゲ Application A-500 (KAO. Corp. SA's manufacturings), ニ ユ one コ one Le 740 (Japanese emulsifying agent company manufacturing) etc.
As the long-chain branched-chain alkyl in the POEPOA long-chain branched-chain alkyl ether, can enumerate for example branched-chain alkyl of carbonatoms 20～30, for example Isoeicosane base, octyl-decyl, different heneicosyl, different docosyl, different tricosyl, isotetracosane base, decyl tetradecyl, different pentacosyl, decyl pentadecyl, isohexacosane base, dodecyl tetradecyl, different heptacosyl, different octacosyl, different nonacosyl, squalane base etc.As the commercially available product of POEPOA long-chain branched-chain alkyl ether, can enumerate ユ ニ Le one Block MT-0620B, ユ ニ Le one Block 20MT-2000B, ユ ニ Le one Block 50MT-2200B (above) etc. by Nof Corp.'s manufacturing.
Molecular weight as POP is the commercially available product of the POEPOP condenses more than 2750, can enumerate プ Le ロ ニ Star Network L-101, プ Le ロ ニ Star Network P-103, プ Le ロ ニ Star Network F-108, プ Le ロ ニ Star Network L-121, プ Le ロ ニ Star Network L-122 (above made by rising sun electrification company), BLAUNON P-303, BLAUNON P-304 (above made by blue or green wood oil fat company) etc.
As the long-chain branched-chain alkyl in the POE long-chain branched-chain alkyl ether, the branched-chain alkyl of preferred carbonatoms 13～30.Especially, as the POE long-chain branched-chain alkyl ether among the present invention, the carbonatoms of preferred long-chain branched-chain alkyl be 21～30 or the HLB value be POE long-chain branched-chain alkyl alkyl oxide more than 15.0.As the branched-chain alkyl of carbonatoms 13～30, can enumerate for example isotridecyl, different tetradecyl, different pentadecyl, isocetyl, hexyl decyl, different heptadecyl, isooctadecane base, norphytane base, Isoeicosane base, octyl group dodecyl, different heneicosyl, different docosyl, different tricosyl, isotetracosane base, decyl tetradecyl, different pentacosyl, decyl pentadecyl, isohexacosane base, dodecyl tetradecyl, different heptacosyl, different octacosyl, different nonacosyl, squalane base etc.As the commercially available product of POE long-chain branched-chain alkyl ether, can enumerate EMALEX 1615, EMALEX 1620, EMALEX 1625, EMALEX 1815, EMALEX 1820, EMALEX 1825, EMALEX OD-16, EMALEX OD-25, EMALEX 2415, EMALEX 2420, EMALEX 2425, EMALEX 2515, EMALEX 2520, EMALEX 2525 (above), ノ イ ゲ Application TDS-500F (above) etc. by the manufacturing of the first industrial drugmaker by the manufacturing of nihon emulsion company.
As the concentration of the surfactant B in the RLP-C mensuration of the present invention, then be not particularly limited so long as can carry out the concentration of RLP-C mensuration of the present invention, the concentration in the preferred reaction liquid is 0.001～20%, more preferably 0.01～10%.
In addition, HLB value representation amphipathic property equilibrium value.The tensio-active agent that uses among the present invention for example POE encircles the HLB value of phenyl ether more, can calculate by the tensio-active agent handbook (interfacial activity drug Ha Application De Block Star Network) (work such as Jitian Shi Hang, industrial books Co., Ltd.) or the method for new surface active agents (new interfacial agent) (the rich work of hole mouth, three is published Co., Ltd. altogether) record.In addition, as this HLB value, also can utilize the HLB value of putting down in writing in the product innovation handbook of various tensio-active agent manufacturers or the brochure.
Reagent removed by hydrogen peroxide so long as the determination of peroxide reaction that will be changed into by the hydrogen peroxide that the lipoprotein cholesterol beyond the RLP-C generates being generated by RLP-C not have the reagent of the material of influence then to be not particularly limited, and can enumerate the reagent etc. that contains catalatic reagent, contains one of two kinds of oxidative coupling type chromogens of use in peroxidase and the reaction of oxidative coupling type color development.Particularly, coupling agent, phenyl amines or the phenol in the illustration " oxidative coupling color development type chromogen " described later.In this specification sheets, term " another oxidative coupling type chromogen " uses in pairs with term " one of two kinds of oxidative coupling type chromogens that use in the reaction of oxidative coupling type color development ", for example, the former represents under the situation of coupling agent, the latter represents phenol or phenyl amines, the former represents that the latter represents coupling agent under the situation of phenol or phenyl amines.
Use contains catalatic reagent when removing reagent as hydrogen peroxide, the preferred 0.001～5000kU/L of catalatic concentration, more preferably 0.01～1000kU/L.
When using one of two kinds of oxidative coupling type chromogens using in for example peroxidase and the oxidative coupling type color development reaction to remove reagent as hydrogen peroxide, the preferred 0.01～500kU/L of the concentration of peroxidase, more preferably 1～100kU/L.Preferred 0.01～the 10g/L of concentration of one of two kinds of oxidative coupling type chromogens that use in the reaction of oxidative coupling type color development.
Hydrogen peroxide determination reagent is the reagent that the hydrogen peroxide that is used for generating changes the material that can detect into.As the material that can detect, can enumerate for example pigment, light etc., preferred pigment.The material that can detect is under the situation of pigment, and hydrogen peroxide determination contains peroxidation active substances such as oxidation color development type chromogen and peroxidase with reagent.As oxidation color development type chromogen, can enumerate oxidation color development type chromogen for example described later.The material that can detect is that hydrogen peroxide determination contains chemiluminescent substance with reagent under the situation of light.As chemiluminescent substance, can enumerate for example luminol,3-aminophthalic acid cyclic hydrazide, different luminol,3-aminophthalic acid cyclic hydrazide, lucigenin, acridinium ester etc.
As hydrogen peroxide determination reagent, when use contains the reagent of peroxidation active substances such as oxidation color development type chromogen and peroxidase, hydrogen peroxide generates pigment with the reaction of oxidation color development type chromogen in the presence of the peroxidation active substance, by the pigment of quantitative generation, quantitative hydrogen peroxide.In addition, when using the hydrogen peroxide determination contain chemiluminescent substance use reagent, hydrogen peroxide and chemiluminescent substance qualitative response generation light, by the light of quantitative generation, quantitative hydrogen peroxide.
As oxidation color development type chromogen, can enumerate for example uncolored type chromogen, oxidative coupling color development type chromogen etc.
The uncolored type chromogen is the material that changes pigment in the presence of peroxidation active substances such as hydrogen peroxide and peroxidase separately into.Particularly; can enumerate 10-N-carboxymethylamino formyl radical-3; two (the dimethylamino)-10H-thiodiphenylamine (CCAP) of 7-, 10-N-methylamino formyl radical-3; two (the dimethylamino)-10H-thiodiphenylamine (MCDP) of 7-, N-(carboxymethylamino carbonyl)-4; 4 '-two (dimethylamino) diphenylamine sodium salt (DA-64), 4,4 '-two (dimethylamino) diphenylamine, two [two (4-chloro-phenyl-) methyl of 3--4-dimethylamino phenyl] amine (BCMA) etc.
Oxidative coupling color development type chromogen is the material that generates pigment in the presence of peroxidation active substances such as hydrogen peroxide and peroxidase by the oxidative coupling of two kinds of compounds.
As two kinds of combination of compounds, can enumerate the combination etc. of combination, coupling agent and the phenol of coupling agent and phenyl amines.As coupling agent, can enumerate for example 4-aminoantipyrene (4-AA), 3-methyl-2-[4-morpholinodithio quinoline ketone hydrazine etc.As phenyl amines; can enumerate N-(3-sulfo group propyl group) aniline; N-ethyl-N-(2-hydroxyl-3-sulfo group propyl group)-3-monomethylaniline (TOOS); N-ethyl-N-(2-hydroxyl-3-sulfo group propyl group)-3; 5-xylidine (MAOS); N-ethyl-N-(2-hydroxyl-3-sulfo group propyl group)-3; 5-dimethoxyaniline (DAOS); N-ethyl-N-(3-sulfo group propyl group)-3-monomethylaniline (TOPS); N-(2-hydroxyl-3-sulfo group propyl group)-3; 5-dimethoxyaniline (HDAOS); N; N-dimethyl-3-monomethylaniline; N; N-two (3-sulfo group propyl group)-3; the 5-dimethoxyaniline; N-ethyl-N-(3-sulfo group propyl group)-3-anisidine; N-ethyl-N-(3-sulfo group propyl group) aniline; N-ethyl-N-(3-sulfo group propyl group) 3; the 5-dimethoxyaniline; N-(3-sulfo group propyl group)-3; the 5-dimethoxyaniline; N-ethyl-N-(3-sulfo group propyl group) 3; the 5-xylidine; N-ethyl-N-(2-hydroxyl-3-sulfo group propyl group) aniline; N-ethyl-N-(3-aminomethyl phenyl)-N '-succinyl-quadrol (EMSE); N-ethyl-N-(3-aminomethyl phenyl)-N '-acetylethylenediamine; N-ethyl-N-(2-hydroxyl-3-sulfo group propyl group)-4-fluoro-3,5-dimethoxyaniline (F-DAOS) etc.As phenol, can enumerate phenol, 4-chlorophenol, 3-methylphenol, 3-hydroxyl-2,4,6-Triiodobenzoic acid (HTIB) etc.
In determination of peroxide, the concentration of peroxidation active substance is being used under the situation of peroxidase as the peroxidation active substance preferred 1～100kU/L so long as the concentration that is suitable for measuring then is not particularly limited.In addition, the concentration of oxidation color development type chromogen is so long as the concentration that is suitable for measuring then is not particularly limited preferred 0.01～10g/L.
Among the present invention, the mensuration of RLP-C is preferably carried out in aqueous medium.As aqueous medium, can enumerate for example deionized water, distilled water, damping fluid etc., preferred buffer.As the buffer reagent that uses in the damping fluid, can enumerate for example three (methylol) aminomethane buffer agent, phosphoric acid buffer agent, borate buffer, Good ' s buffer reagent etc.
As Good ' s damping fluid, for example can enumerate: 2-morpholino ethane sulfonic acid (MES), two (2-hydroxyethyl) imino-s three (methylol) methane (Bisi-Tris), N-(2-acetamido) iminodiethanoic acid (ADA), piperazine-N, N '-two (2 ethane sulfonic aicd) (PIPES), N-(2-acetamido)-2-aminoethane sulphonic acid (ACES), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO), N, two (2-the hydroxyethyl)-2-aminoethane sulphonic acid (BES) of N-, 3-morpholino propane sulfonic acid (MOPS), N-[three (methylol) methyl]-2-aminoethane sulphonic acid (TES), 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethane sulfonic acid (HEPES), 3-[N, two (2-hydroxyethyl) amino of N-]-2-hydroxypropanesulfonic acid (DIPSO), N-[three (methylol) methyl]-2-hydroxyl-3-amino propane sulfonic acid (TAPSO), piperazine-N, N '-two (2-hydroxypropanesulfonic acid) (POPSO), 3-[4-(2-hydroxyethyl)-1-piperazinyl]-2-hydroxypropanesulfonic acid (HEPPSO), 3-[4-(2-hydroxyethyl)-1-piperazinyl] propane sulfonic acid [(H) EPPS], N-[three (methylol) methyl] glycine (Tricine), N, two (2-hydroxyethyl) glycine (Bicine) of N-, N-three (methylol) methyl-3-amino propane sulfonic acid (TAPS), N-cyclohexyl-2-aminoethane sulphonic acid (CHES), N-cyclohexyl-3-amino-2-hydroxypropanesulfonic acid (CAPSO), N-cyclohexyl-3-amino propane sulfonic acid (CAPS) etc.The concentration of damping fluid is so long as the concentration of be fit to measuring then is not particularly limited preferred 0.001～2.0mol/L, more preferably 0.005～1.0mol/L.
Below, to the measuring method of RLP-C of the present invention, measure reagent and measure test kit and be specifically described.
(measuring method of RLP-C)
RLP-C measuring method of the present invention, comprise the following steps: that (i) is in containing the aqueous medium of surfactant A, make the reaction of a corpse or other object for laboratory examination and chemical testing and rCO or cholesterol dehydrogenase and oxidized coenzyme, remove the step of the cholesterol in the RLP lipoprotein in addition; (ii) make in the reaction solution after the cholesterol in the lipoprotein of in abovementioned steps (i), having removed beyond the RLP and contain surfactant B, and make cholesterol ester hydrolase and rCO or cholesterol ester hydrolase, cholesterol dehydrogenase and oxidized coenzyme reaction, generate the step of hydrogen peroxide or reduced coenzyme; (iii) be determined at the (ii) middle hydrogen peroxide that generates of abovementioned steps or the step of reduced coenzyme.
In the method for the present invention, step (i) can be in the presence of polyanion, in the presence of polyanion and the divalent-metal ion or be selected from the presence of the antibody in the group of forming by anti-apolipoprotein B antibody and anti-apo E antibody and carry out.Under the condition that polyanion exists, as surfactant A preferred surfactant (A1).In addition, in the presence of polyanion and the divalent-metal ion or be selected from the presence of the antibody in the group of forming by anti-apolipoprotein B antibody and anti-apo E antibody,, preferably use tensio-active agent (A2) as surfactant A.
As the step of the cholesterol in the lipoprotein of removing beyond the RLP, can enumerate the hydrogen peroxide of for example removing generation in step of the present invention (i) or the step of reduced coenzyme.
Remove the step of the middle hydrogen peroxide that generates of step of the present invention (i), can be set forth in the method for one of the method that makes the catalase coexistence in the step (i), two kinds of oxidative coupling type chromogens that use use in peroxidase and the reaction of oxidative coupling type color development etc.
Remove the step of the reduced coenzyme that generates in the step of the present invention (i), can be set forth in the step (i) and make the reduced coenzyme oxydase act on reduced coenzyme and generate hydrogen peroxide, with above-mentioned method of similarly removing.
In addition, in the present invention, remove the step of the cholesterol in the lipoprotein beyond the RLP in the step (i), and be not only that the hydrogen peroxide that will be generated by this cholesterol or reduced coenzyme change other material into and the step eliminated.Under the situation that hydrogen peroxide and reduced coenzyme can be measured respectively, the hydrogen peroxide or the reduced coenzyme that there is no need to be generated by this cholesterol change other material into.For example, make cholesterol in the lipoprotein beyond the RLP generate reduced coenzyme, the cholesterol among the RLP be generated under the situation of hydrogen peroxide in step in (ii) in step (i), the reduced coenzyme that there is no need to generate in the step (i) changes other material into.In addition, the present invention also comprises hydrogen peroxide or the reduced coenzyme that generates in the determination step (i), and based on (ii) middle hydrogen peroxide or the reduced coenzyme that generates of resulting measured value calculation procedure, determines the RLP-C in the corpse or other object for laboratory examination and chemical testing thus.
The measuring method of RLP-C more specifically of the present invention can be enumerated for example mode of following mode.
Measuring method
(1) corpse or other object for laboratory examination and chemical testing and rCO or rCO and cholesterol ester hydrolase are reacted in containing surfactant A and catalatic aqueous medium, and eliminate the hydrogen peroxide that generates, reaction solution after hydrogen peroxide is eliminated with comprise rCO and cholesterol ester hydrolase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, react if desired to generate hydrogen peroxide, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(2) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxidasic enzyme group react in containing surfactant A and catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(3) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxidasic enzyme group react in containing surfactant A and catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(4) make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens of rCO and cholesterol ester hydrolase use in containing the reaction of surfactant A and peroxidase and oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise rCO and cholesterol ester hydrolase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(5) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme uses in containing the reaction of surfactant A and peroxidase and oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(6) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme uses in containing the reaction of surfactant A and peroxidase and oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(7) corpse or other object for laboratory examination and chemical testing and rCO or rCO and cholesterol ester hydrolase are reacted in containing tensio-active agent (A1), polyanion and catalatic aqueous medium, and the hydrogen peroxide of elimination generation, reaction solution after hydrogen peroxide is eliminated with comprise rCO and cholesterol ester hydrolase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, react if desired to generate hydrogen peroxide, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(8) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A1), react in polyanion and the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(9) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A1), react in polyanion and the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(10) make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps rCO and cholesterol ester hydrolase are containing tensio-active agent (A1), react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that use in polyanion and peroxidase and the reaction of oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise rCO and cholesterol ester hydrolase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(11) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A1), react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that use in polyanion and peroxidase and the reaction of oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(12) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A1), react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that use in polyanion and peroxidase and the reaction of oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(13) make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps rCO and cholesterol ester hydrolase are containing tensio-active agent (A2), polyanion, react in divalent-metal ion and the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise rCO and cholesterol ester hydrolase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(14) make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps rCO and cholesterol ester hydrolase are containing tensio-active agent (A2), anti-apolipoprotein B antibody and/or anti-apo E antibody, and react in the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise rCO and cholesterol ester hydrolase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(15) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), polyanion, react in divalent-metal ion and the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(16) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), anti-apolipoprotein B antibody and/or anti-apo E antibody, and react in the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(17) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), polyanion, react in divalent-metal ion and the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(18) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), anti-apolipoprotein B antibody and/or anti-apo E antibody, and react in the catalatic aqueous medium, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have the enzyme group of phosphatide lytic enzyme in the aqueous medium that contains surfactant B and hydrogen peroxide enzyme inhibitors, to react the generation hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(19) make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps rCO and cholesterol ester hydrolase are containing tensio-active agent (A2), polyanion, react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that use in divalent-metal ion and peroxidase and the reaction of oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise rCO and cholesterol ester hydrolase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(20) make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps rCO and cholesterol ester hydrolase are containing tensio-active agent (A2), anti-apolipoprotein B antibody and/or anti-apo E antibody, and react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens of use in the reaction of peroxidase and oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise rCO and cholesterol ester hydrolase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(21) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), polyanion, react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that use in divalent-metal ion and peroxidase and the reaction of oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(22) make a corpse or other object for laboratory examination and chemical testing and comprise cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), anti-apolipoprotein B antibody and/or anti-apo E antibody, and react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens of use in the reaction of peroxidase and oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(23) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), polyanion, react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens that use in divalent-metal ion and peroxidase and the reaction of oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
(24) make a corpse or other object for laboratory examination and chemical testing and comprise rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme, perhaps comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, the oxidasic enzyme group of oxidized coenzyme and reduced coenzyme is being contained tensio-active agent (A2), anti-apolipoprotein B antibody and/or anti-apo E antibody, and react in the aqueous medium of the combination of one of two kinds of oxidative coupling type chromogens of use in the reaction of peroxidase and oxidized form color development, and the hydrogen peroxide of elimination generation, make the reaction solution after hydrogen peroxide is eliminated then and comprise cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase, also have enzyme group reaction in the aqueous medium that contains surfactant B and another oxidative coupling type chromogen of phosphatide lytic enzyme to generate hydrogen peroxide if desired, by measuring the RLP-C concentration in this hydrogen peroxide calculating corpse or other object for laboratory examination and chemical testing that generates.
Reaction in this assay method was for example carried out under 10～50 ℃, preferred 20～40 ℃ 1～60 minute, preferred 2～30 minutes.The amount of the hydrogen peroxide that generates for example can directly be passed through the hydrogen peroxide determination of electrode, also can measure with reagent by hydrogen peroxide determination.
(RLP-C measures reagent)
RLP-C of the present invention measures and uses reagent, can be used for RLP-C of the present invention and measure.
RLP-C of the present invention measures and uses reagent, can be listed below reagent.
Containing cholesterol ester hydrolase, rCO, hydrogen peroxide removes RLP-C in the corpse or other object for laboratory examination and chemical testing of reagent, hydrogen peroxide assay reagents, surfactant A and surfactant B and measures and use reagent.
Remove reagent as hydrogen peroxide, can enumerate the reagent that contains one of two kinds of oxidative coupling type chromogens using in the reaction of peroxidase and oxidative coupling type color development or contain catalatic reagent.
In addition, RLP-C of the present invention measures and can be listed below reagent with reagent.
Containing RLP-C in the corpse or other object for laboratory examination and chemical testing of cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme, surfactant A and surfactant B measures and uses reagent.
In addition, RLP-C of the present invention measures and can enumerate following reagent with reagent.
Containing cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme, reduced coenzyme oxydase, hydrogen peroxide removes RLP-C in the corpse or other object for laboratory examination and chemical testing of reagent, hydrogen peroxide assay reagents, surfactant A and surfactant B and measures and use reagent.
Remove reagent as hydrogen peroxide, can enumerate the reagent that contains one of two kinds of oxidative coupling type chromogens using in the reaction of peroxidase and oxidative coupling type color development or contain catalatic reagent etc.
These reagent can further contain polyanion.At this moment, as the surfactant A that contains, preferred surfactant (A1).
In addition, these reagent can contain polyanion and divalent-metal ion or be selected from antibody in the group of being made up of anti-apolipoprotein B antibody and anti-apo E antibody.At this moment, as the surfactant A that contains, preferred surfactant (A2).
In addition, can contain the phosphatide lytic enzyme.
RLP-C more specifically of the present invention measures reagent can enumerate for example reagent of following mode, but these reagent to scope of the present invention without any restriction.Among the present invention,, can use one or more tensio-active agents of from each group, selecting as surfactant A, tensio-active agent (A1), tensio-active agent (A2) and surfactant B.
Reagent 1
The reagent that contains cholesterol ester hydrolase, rCO, catalase, hydrogen peroxide enzyme inhibitors, surfactant A and surfactant B.
Reagent 2
Contain cholesterol ester hydrolase, rCO, catalase, hydrogen peroxide enzyme inhibitors, surfactant A, surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 3
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A and surfactant B.
Reagent 4
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A, surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 5
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A and surfactant B.
Reagent 6
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A, surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 7
The reagent that contains cholesterol ester hydrolase, rCO, peroxidase, oxidative coupling type chromogen, surfactant A and surfactant B.
Reagent 8
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A and surfactant B.
Reagent 9
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A and surfactant B.
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A and surfactant B.
Reagent 11
Contain cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A, surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 12
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A and surfactant B.
Reagent 13
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A, surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 14
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A and surfactant B.
Reagent 15
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, catalase, hydrogen peroxide enzyme inhibitors, surfactant A, surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 16
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, peroxidase, oxidative coupling type chromogen, surfactant A and surfactant B.
Reagent 17
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, peroxidase, oxidative coupling type chromogen, surfactant A and surfactant B.
Reagent 18
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, peroxidase, oxidative coupling type chromogen, surfactant A and surfactant B.
Reagent 19
The reagent that contains cholesterol ester hydrolase, rCO, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1) and surfactant B.
Contain cholesterol ester hydrolase, rCO, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 21
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1) and surfactant B.
Reagent 22
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 23
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1) and surfactant B.
Reagent 24
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 25
The reagent that contains cholesterol ester hydrolase, rCO, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1) and surfactant B.
Reagent 26
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1) and surfactant B.
Reagent 27
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1) and surfactant B.
Reagent 28
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1) and surfactant B.
Reagent 29
Contain cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1), surfactant B and hydrogen peroxide determination reagent with reagent.
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1) and surfactant B.
Reagent 31
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 32
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1) and surfactant B.
Reagent 33
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A1), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 34
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1) and surfactant B.
Reagent 35
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1) and surfactant B.
Reagent 36
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1) and surfactant B.
Reagent 37
The reagent that contains cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 38
Contain cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 39
The reagent that contains cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Contain cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 41
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 42
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 43
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 44
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 45
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 46
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 47
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 48
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 49
The reagent that contains cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
The reagent that contains cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 51
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 52
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 53
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 54
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 55
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 56
Contain cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 57
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 58
Contain cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 59
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 61
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 62
Contain cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 63
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 64
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, divalent-metal ion, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 65
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2) and surfactant B.
Reagent 66
Contain cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, hydrogen peroxide enzyme inhibitors, tensio-active agent (A2), surfactant B and hydrogen peroxide determination reagent with reagent.
Reagent 67
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 68
The reagent that contains cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 69
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 70
The reagent that contains cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 71
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
Reagent 72
The reagent that contains cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2) and surfactant B.
(RLP-C measures and uses test kit)
RLP-C of the present invention measures and uses reagent, can preserve, circulate and use with the form of test kit.As the form of test kit, then be not particularly limited so long as can realize the form of RLP-C assay method of the present invention, can be arbitrary forms such as double reagent type, three reagent types, preferred double reagent type.
RLP-C of the present invention measures and uses test kit, can enumerate following test kit.
A kind of RLP-C measures and uses test kit, contains first reagent and second reagent, and described first reagent contains rCO, hydrogen peroxide is removed reagent and surfactant A, and described second reagent contains hydrogen peroxide determination with reagent and surfactant B; And make among any one or two in first reagent, second reagent and contain cholesterol ester hydrolase.
In addition, RLP-C of the present invention measures and uses test kit, can enumerate following test kit.
A kind of RLP-C measures and uses test kit, contains first reagent and second reagent, and described first reagent contains cholesterol dehydrogenase, oxidized coenzyme and surfactant A, and described second reagent contains surfactant B; And make among any one or two in first reagent, second reagent and contain cholesterol ester hydrolase.
In addition, RLP-C of the present invention measures and uses test kit, can enumerate following test kit.
A kind of RLPC measures and uses test kit, contains first reagent, and described first reagent contains cholesterol dehydrogenase, oxidized coenzyme reduced coenzyme oxydase, hydrogen peroxide removes reagent and surfactant A; With second reagent, described second reagent contains hydrogen peroxide assay reagents and surfactant B; And make any one or two of first reagent, second reagent contain cholesterol ester hydrolase.
In these test kits, can further contain polyanion.At this moment, as the surfactant A that contains, preferred surfactant (A1).
In addition, in these test kits, can contain polyanion and divalent-metal ion or be selected from antibody in the group of forming by anti-apolipoprotein B antibody and anti-apo E antibody.At this moment, as the surfactant A that contains, preferred surfactant (A2).
In addition, can contain the phosphatide lytic enzyme.
Measure with in the test kit at the double reagent type RLP-C that comprises first reagent and second reagent, contain rCO in first reagent, but also can first reagent and second reagent in all contain rCO.
Surfactant A, tensio-active agent (A1) and tensio-active agent (A2) preferably are contained in first reagent.
Contain among any one or two that cholesterol ester hydrolase can be in first reagent, second reagent, all contain in preferred first reagent and second reagent.
Cholesterol dehydrogenase is included in first reagent, but also can first reagent and second reagent in all contain.
The reduced coenzyme oxydase is included in first reagent, but also can first reagent and second reagent in all contain.
Oxidized coenzyme is included in first reagent, but also can first reagent and second reagent in all contain.
The catalase that uses in the elimination of the hydrogen peroxide that first reaction produces preferably is contained in first reagent, and the hydrogen peroxide enzyme inhibitors preferably is contained in second reagent.
The combination of one of two kinds of oxidative coupling type chromogens that use in use peroxidase and the reaction of oxidative coupling type color development in the elimination of the hydrogen peroxide that first reaction produces preferably is contained in first reagent.
Polyanion preferably is contained in first reagent.
Divalent-metal ion preferably is contained in first reagent.
Anti-apolipoprotein B antibody can contain in two of first reagent and second reagent, but preferably is contained in first reagent.
Anti-apo E antibody can contain in two of first reagent and second reagent, but preferably is contained in first reagent.
Surfactant B and hydrogen peroxide determination preferably are contained in second reagent with reagent.
Contain among any one or two that the phosphatide lytic enzyme can be in first reagent, second reagent, but preferably be contained in second reagent.
RLP-C of the present invention measures and uses test kit, for example can enumerate the test kit of following form, but these to scope of the present invention without any restriction.
Test kit 1
First reagent
RCO, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 2
First reagent
RCO, cholesterol ester hydrolase, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 3
First reagent
RCO, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 4
First reagent
RCO, cholesterol ester hydrolase, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 5
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 6
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 7
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 8
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 9
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 11
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 12
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 13
First reagent
One of rCO, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 14
First reagent
One of cholesterol ester hydrolase, rCO, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 15
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 16
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 17
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, rCO, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme, oxidized coenzyme, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 18
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, rCO, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme, oxidized coenzyme, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 19
First reagent
RCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B
First reagent
Cholesterol ester hydrolase, rCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 21
First reagent
RCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 22
First reagent
Cholesterol ester hydrolase, rCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 23
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 24
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 25
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 26
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 27
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 28
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 29
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 31
First reagent
One of rCO, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 32
First reagent
One of cholesterol ester hydrolase, rCO, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 33
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 34
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 35
First reagent
One of rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 36
First reagent
One of cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 37
First reagent
RCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 38
First reagent
Cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 39
First reagent
RCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
First reagent
Cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 41
First reagent
RCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 42
First reagent
Cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 43
First reagent
RCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 44
First reagent
Cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 45
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 46
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 47
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 48
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 49
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 51
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 52
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 53
First reagent
One of rCO, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 54
First reagent
One of cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 55
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 56
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 57
First reagent
One of rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 58
First reagent
One of cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 59
First reagent
One of rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
First reagent
One of cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, surfactant B, another oxidative coupling type chromogen
Test kit 61
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 62
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 63
First reagent
One of rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 64
First reagent
One of cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, surfactant B, another oxidative coupling type chromogen
Test kit 65
First reagent
RCO, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 66
First reagent
RCO, cholesterol ester hydrolase, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 67
First reagent
RCO, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 68
First reagent
RCO, cholesterol ester hydrolase, catalase, surfactant A
Second reagent
RCO, cholesterol ester hydrolase, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 69
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 70
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 71
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 72
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 73
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 74
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 75
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 76
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, catalase, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 77
First reagent
One of rCO, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 78
First reagent
One of cholesterol ester hydrolase, rCO, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 79
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 80
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 81
First reagent
One of rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 82
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, peroxidase, oxidative coupling type chromogen, surfactant A
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 83
First reagent
RCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 84
First reagent
Cholesterol ester hydrolase, rCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 85
First reagent
RCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 86
First reagent
Cholesterol ester hydrolase, rCO, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 87
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 88
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 89
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 90
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 91
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 92
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 93
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 94
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, catalase, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 95
First reagent
One of rCO, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 96
First reagent
One of cholesterol ester hydrolase, rCO, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 97
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 98
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 99
First reagent
One of rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 100
First reagent
One of cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A1)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 101
First reagent
RCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 102
First reagent
Cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 103
First reagent
RCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 104
First reagent
Cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 105
First reagent
RCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 106
First reagent
Cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 107
First reagent
RCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 108
First reagent
Cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 109
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 110
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 111
First reagent
Cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 112
First reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 113
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 114
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B
Test kit 115
First reagent
RCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 116
First reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, catalase, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, hydrogen peroxide enzyme inhibitors, surfactant B, hydrogen peroxide determination reagent
Test kit 117
First reagent
One of rCO, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 118
First reagent
One of cholesterol ester hydrolase, rCO, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 119
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 120
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 121
First reagent
One of rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 122
First reagent
One of cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, polyanion, divalent-metal ion, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 123
First reagent
One of rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 124
First reagent
One of cholesterol ester hydrolase, rCO, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 125
First reagent
One of cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 126
First reagent
One of cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 127
First reagent
One of rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
Test kit 128
First reagent
One of cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, anti-apolipoprotein B antibody and/or anti-apo E antibody, peroxidase, oxidative coupling type chromogen, tensio-active agent (A2)
Second reagent
Cholesterol ester hydrolase, rCO, cholesterol dehydrogenase, reduced coenzyme oxydase, oxidized coenzyme, phosphatide lytic enzyme, surfactant B, another oxidative coupling type chromogen
RLP-C of the present invention measures with in reagent and the test kit, can use enumerate in the aforesaid RLP-C measuring method of the present invention, rCO, surfactant A, tensio-active agent (A1), tensio-active agent (A2), polyanion, divalent-metal ion, anti-apolipoprotein B antibody, anti-apo E antibody, catalase, peroxidase, oxidative coupling type chromogen, cholesterol ester hydrolase, surfactant B, hydrogen peroxide determination be with reagent and phosphatide lytic enzyme.
RLP-C of the present invention measures with in reagent and the test kit, can contain aqueous medium, stablizer, sanitas as required, influence material remover, reaction promotor etc.As aqueous medium, can enumerate for example aforesaid aqueous medium etc.As stablizer, can enumerate for example ethylenediamine tetraacetic acid (EDTA) (EDTA), sucrose, calcium chloride etc.As sanitas, can enumerate for example sodiumazide, バ イ オ エ one ス (Bioace), antibiotics etc.As influencing the material remover, can enumerate the Vitamin C oxidase that for example is used to eliminate the xitix influence etc.As reaction promotor, can enumerate for example enzyme such as colipase, salts such as sodium sulfate, sodium-chlor etc.
RLP-C of the present invention measures with reagent and measures and use test kit, can be cryodesiccated state, also can be the state that is dissolved in aqueous medium.When using the reagent of lyophilize state to measure RLP-C in the corpse or other object for laboratory examination and chemical testing, this agent dissolves is used in aqueous medium.
RLP-C of the present invention measures with reagent and measures with the cholesterol ester hydrolase in the test kit, the content of rCO, and preferably the concentration of dissolved state is the content of 0.001～2000U/mL, the more preferably content of 0.005～1000U/mL in water-based Jie property.
RLP-C of the present invention measures with reagent and measures with the oxidasic content of cholesterol dehydrogenase, reduced coenzyme in the test kit, and preferably the concentration of dissolved state is the content of 0.001～2000U/mL, the more preferably content of 0.005～1000U/mL in water-based Jie property.
RLP-C of the present invention measures with reagent and measures the content of using the oxidized coenzyme in the test kit, and preferably the concentration of dissolved state is the content of 0.01～500mmol/L in water-based Jie property, more preferably the content of 0.1～100mmol/L.
RLP-C of the present invention measures with reagent and measures content with the phosphatide lytic enzyme in the test kit, and preferably the concentration of dissolved state is the content of 0.01～1000U/mL, the more preferably content of 0.1～500U/mL in water-based Jie property.
RLP-C of the present invention measures with reagent and measures content with surfactant A, tensio-active agent (A1) and tensio-active agent (A2) in the test kit, and preferably the concentration of dissolved state is 0.0001～10% content, more preferably 0.001～5% content in water-based Jie property.
RLP-C of the present invention measures with reagent and measures content with the polyanion in the test kit, and preferably the concentration of dissolved state is 0.0001～30% content, more preferably 0.001～3% content in water-based Jie property.
RLP-C of the present invention measures with reagent and measures content with the divalent-metal ion in the test kit, and preferably the concentration of dissolved state is 0.001～30% content, more preferably 0.01～3% content in water-based Jie property.
RLP-C of the present invention measures with reagent and measures content with the surfactant B in the test kit, and preferably the concentration of dissolved state is 0.001～40% content, more preferably 0.01～20% content in water-based Jie property.
Below, be described more specifically the present invention by embodiment, but these embodiment to scope of the present invention without any restriction.In addition, in present embodiment and reference example, use the reagent and the enzyme of following manufacturers.
MOPS (manufacturing of colleague's chemistry institute company), EMSE (manufacturing of グ イ ト one ケ ミ Star Network ス company), TOOS (manufacturing of colleague's chemistry institute company), sodium sulfate (manufacturing of Northeast chemical company), dextran sulfate sodium (the sugared industry of name company makes), magnesium nitrate hexahydrate (company makes with the pure pharmaceutical worker's industry of light), 4-aminoantipyrene (the Saitama capital changes into company and makes), peroxidase (Japan's textile company is made), CHO-CE (rCO; The manufacturing of キ Star コ one マ Application company), LPL-312 (cholesterol ester hydrolase; Japan's textile company manufacturing), the LPL (cholesterol ester hydrolase of chemically modified; Our company's goods), LPBP (cholesterol ester hydrolase; The manufacturing of company of Asahi Chemical Industry), CEN (cholesterol ester hydrolase; The manufacturing of company of Asahi Chemical Industry), COE-301 (cholesterol ester hydrolase; Japan's textile company manufacturing), PLDP (phosphatide lytic enzyme; The manufacturing of company of Asahi Chemical Industry), PLA2 (phosphatide lytic enzyme; The manufacturing of company of Asahi Chemical Industry), Vitamin C oxidase (manufacturing of company of Asahi Chemical Industry).
エ マ Le ゲ Application B-66[surfactant A: the HLB value is encircled phenyl ether less than 16.0 POE more; KAO. Corp. SA makes], ニ ユ one カ Le ゲ Application GP-120[surfactant A: POEPOA encircles phenyl ether more; This grease of bamboo company makes], ニ ユ one コ one Le 2608-F[surfactant A: POEPOA encircles phenyl ether more; Japan emulsifying agent company makes], プ Le ロ ニ Star Network TR-704[surfactant A: quadrol-POEPOP condenses; Rising sun electrification company makes], エ チ レ Application ジ ア ミ Application PO40EO40[surfactant A: quadrol-POEPOP condenses; Nof Corp. makes], プ ラ イ サ one Off A219B[surfactant A: the POE alkyl ether phosphate; The first industrial drugmaker makes], モ ノ ゲ Application 170T[surfactant A: higher alcohol sulfate salt; The first industrial drugmaker makes], ニ Star コ one Le NP-10[surfactant A: the POE alkyl phenyl ether; Day Optical Chemical Company makes], Ha イ テ ノ one Le N-07[surfactant A: POE alkyl phenyl ether sulfuric acid; The first industrial drugmaker makes], ノ イ ゲ Application SD300[surfactant A: POE short-chain branches alkyl oxide; The first industrial drugmaker makes], パ one ソ Off ト EF[surfactant A: the POE alkyl ether sulphate salts; Nof Corp. makes], パ one ソ Off ト EP[surfactant A: the POE alkyl ether sulphate salts; Nof Corp. makes], パ one ソ Off ト EFT[surfactant A: the POE alkyl ether sulphate salts; Nof Corp. makes], パ one ソ Off ト EDO[surfactant A: the POE alkyl ether sulphate salts; Nof Corp. makes], ニ ユ one コ one Le 707-SF[surfactant A: POE encircles the phenyl ether sulfuric acid more; Japan emulsifying agent company makes], ニ ユ one コ one Le 707-SFC[surfactant A: POE encircles the phenyl ether sulfuric acid more; Japan emulsifying agent company makes], ニ ユ one コ one Le 723-SF[surfactant A: POE encircles the phenyl ether sulfuric acid more; Japan emulsifying agent company makes], プ Le ロ ニ Star Network L-34[surfactant A: the molecular weight of POP is less than 2750 POEPOP condenses; Rising sun electrification company makes], プ Le ロ ニ Star Network F-68[surfactant A: the molecular weight of POP is less than 2750 POEPOP condenses; Rising sun electrification company makes], エ パ Application 420[surfactant A: the molecular weight of POP is less than 2750 POEPOP condenses; The first industrial drugmaker makes], エ パ Application 108[surfactant A: the molecular weight of POP is less than 2750 POEPOP condenses; The first industrial drugmaker makes], BLAUNON EP-1461[surfactant A: the molecular weight of POP is less than 2750 POEPOP condenses; Blue or green wood oil fat company makes], ノ イ ゲ Application XL-400[surfactant A: POEPOA short-chain branches alkyl oxide; The first industrial drugmaker makes], ノ イ ゲ Application XL-1000F[surfactant A: POEPOA short-chain branches alkyl oxide; The first industrial drugmaker makes], ユ ニ セ one Off PKA-5015[surfactant A: the POEPOA alkyl oxide; Nof Corp. makes], ユ ニ セ one Off PKA-5016[surfactant A: the POEPOA alkyl oxide; Nof Corp. makes], ユ ニ セ one Off LM-2[surfactant A: POEPOA alkyl oxide Nof Corp. makes], the EMALEXDAPE-230[surfactant A: the POEPOA alkyl oxide; Nihon emulsion company makes], BLAUNON BEO-10[surfactant A: the POE Bisphenol A Ether; Blue or green wood oil fat company makes], ニ ユ one コ one Le B4-SN[surfactant A: POE aryl ethers sulfuric acid; Japan emulsifying agent company makes], ニ ユ one コ one Le B13-SN[surfactant A: POE aryl ethers sulfuric acid; Japan emulsifying agent company makes], the BIGCHAP[surfactant A: bile acid derivative; Colleague's chemistry institute company makes], the deoxy-BIGCHAP[surfactant A: bile acid derivative; Colleague's chemistry institute company makes], the CHAPSO[surfactant A: bile acid derivative; Colleague's chemistry institute company makes], BLAUNON MXDA-PO4[surfactant A: the POP m-xylene diamine; Green grass or young crops wood oil fat company makes].
エ チ レ Application ジ ア ミ Application PO40EO40[tensio-active agent (A1): quadrol-POEPOP condenses; Nof Corp. makes], Off ア イ Application サ one Off IDEP-802[tensio-active agent (A1): POEPOP short-chain branches alkyl oxide; Blue or green wood oil fat company makes], プ Le ロ ニ Star Network 25R-2[tensio-active agent (A1): the molecular weight of POP is less than 2750 POEPOP condenses; Rising sun electrification company makes], Sodium cholic acid [tensio-active agent (A1): bile acid derivative; Northeast chemical company makes], Ha イ テ ノ one Le N-07[tensio-active agent (A1): POE alkyl phenyl ether sulfuric acid; The first industrial drugmaker makes], エ マ Le ゲ Application 810[tensio-active agent (A1): the POE alkyl phenyl ether; KAO. Corp. SA makes], ノ イ ゲ Application SD110[tensio-active agent (A1): POE short-chain branches alkyl oxide; The first industrial drugmaker makes], ノ イ ゲ Application SD150[tensio-active agent (A1): POE short-chain branches alkyl oxide; The first industrial drugmaker makes], ノ イ ゲ Application SD300[tensio-active agent (A1): POE short-chain branches alkyl oxide; The first industrial drugmaker makes].
ニ ユ one コ one Le 707-F[tensio-active agent (A2): POEPOA encircles phenyl ether more; Japan emulsifying agent company makes], ニ ユ one コ one Le 707-SN[tensio-active agent (A2): POE encircles the phenyl ether sulfuric acid more; Japan emulsifying agent company makes], EMALEX DAPE-230[tensio-active agent (A2): the POEPOA alkyl oxide; Nihon emulsion company makes], ニ ユ one コ one Le 1105-SN[tensio-active agent (A2): the POE alkyl ether sulphate salts; Japan emulsifying agent company makes], BLAUNON EP-1461[tensio-active agent (A2): the molecular weight of POP is less than 2750 POEPOP condenses; Blue or green wood oil fat company makes], Sodium cholic acid [tensio-active agent (A2): bile acid derivative; Northeast chemical company makes], エ マ Le ゲ Application 810[tensio-active agent (A2): the POE alkyl phenyl ether; KAO. Corp. SA makes], プ Le ロ ニ Star Network TR-704[tensio-active agent (A2): quadrol-POEPOP condenses; Rising sun electrification company makes], Off ア イ Application サ one Off IDEP-802[tensio-active agent (A2): POEPOP short-chain branches alkyl oxide; Green grass or young crops wood oil fat company makes].
プ ロ ノ Application B-208[surfactant B: POEPOB condenses; Nof Corp. makes], BLAUNON TSP-50[surfactant B: HLB value is that the POE 16.0 or more encircles the manufacturing of the blue or green wood oil fat of phenyl ether company more], エ マ Le ゲ Application A-500[surfactant B: the HLB value is that the POE more than 16.0 encircles the manufacturing of phenyl ether KAO. Corp. SA more], ニ ユ one コ one Le 740[surfactant B: the HLB value is that the POE more than 16.0 encircles phenyl ether more; Japan emulsifying agent company makes], ユ ニ Le one Block MT-0620B[surfactant B: POEPOA long-chain branched-chain alkyl ether; Nof Corp. makes], プ Le ロ ニ Star Network F-108[surfactant B: the molecular weight of POP is the POEPOP condenses rising sun electrification company manufacturing 2750 or more], the EMALEX2425[surfactant B: POE long-chain branched-chain alkyl ether; Japan emulsifying agent company makes], ノ イ ゲ Application TDS-500F[surfactant B: POE long-chain branched-chain alkyl ether; The first industrial drugmaker makes], ワ Application グ サ one Off RL-100 (POEPOA alkyl oxide; Green grass or young crops wood oil fat company makes).
Embodiment 1
Preparation comprises following first reagent (reagent A) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
Sodium cholic acid 0.75g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 2
Preparation comprises first following reagent (reagent B) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent B)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
エマルゲンB-66 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 3
Preparation comprises following first reagent (reagent C) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent C)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
プルロニツクTR-704 5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 4
Preparation comprises following first reagent (reagent D) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent D)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
ニ ユ one コ one Le 1105-SN 2g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 5
Preparation comprises following first reagent (reagent E) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 6
Preparation comprises first following reagent (reagent F) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent F)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ニ ユ one カ Le ゲ Application GP-120 4g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 7
Preparation comprises first following reagent (reagent G) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent G)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ニ ユ one コ one Le 2608-F 4g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 8
Preparation comprises first following reagent (reagent H) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent H)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
プルロニツクTR-704 5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 9
Preparation comprises first following reagent (reagent I) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent I)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
プ ラ イ サ one Off A219B 0.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Preparation comprises first following reagent (reagent J) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent J)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
モノゲン170T 3g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 11
Preparation comprises first following reagent (reagent K) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent K)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
LPBP 600kU/L
ニ Star コ one Le NP-10 0.1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 12
Preparation comprises first following reagent (reagent L) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent L)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.05kU/L of chemically modified
Ha イ テ ノ one Le N-07 2g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 13
Preparation comprises first following reagent (reagent M) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent M)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.05kU/L of chemically modified
ノイゲンSD300 3g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 14
Preparation comprises following first reagent (reagent N) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent N)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
エチレンジアミンPO40EO40 0.25g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 15
Preparation comprises first following reagent (reagent O) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent O)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
Off ア イ Application サ one Off IDEP-802 0.1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 16
Preparation comprises first following reagent (reagent P) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent P)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
プルロニツク25R-2 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 17
Preparation comprises first following reagent (reagent Q) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent Q)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
Ha イ テ ノ one Le N-07 2g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 18
Preparation comprises first following reagent (reagent R) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent R)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
ノイゲンSD110 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 19
Preparation comprises first following reagent (reagent S) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent S)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
ノイゲンSD150 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Preparation comprises first following reagent (reagent T) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent T)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
ノイゲンSD300 3g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 21
Preparation comprises first following reagent (reagent U) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent U)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
エマルゲン810 0.05g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 22
Preparation comprises first following reagent (reagent V) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent V)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
COE-301 1kU/L
ニ ユ one コ one Le 707-F 2g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 23
Preparation comprises first following reagent (reagent W) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent W)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
COE-301 1kU/L
ニ ユ one コ one Le 707-SN 4g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 24
Preparation comprises first following reagent (reagent X) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent X)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
COE-301 1kU/L
EMALEX DAPE-230 2g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 25
Preparation comprises first following reagent (reagent Y) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent Y)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
COE-301 1kU/L
ニ ユ one コ one Le 1105-SN 2g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 26
Preparation comprises first following reagent (reagent Z) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent Z)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
The LPL 0.2kU/L of chemically modified
プルロニツクTR-704 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 27
Preparation comprises following first reagent (reagent A A) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A A)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
The LPL 0.2kU/L of chemically modified
Off ア イ Application サ one Off IDEP-802 0.1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 28
Preparation comprises following first reagent (reagent A B) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A B)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
The LPL 0.2kU/L of chemically modified
BLAUNON EP-1461 4g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 29
Preparation comprises following first reagent (reagent A C) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A C)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
The LPL 0.2kU/L of chemically modified
Sodium cholic acid 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Preparation comprises following first reagent (reagent A D) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A D)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
LPBP 600kU/L
エマルゲン810 0.75g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 31
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent b).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent b)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
ユ ニ Le one Block MT-0620B 22.5g/L
Embodiment 32
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent c).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent c)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 0.3kU/L
BLAUNON TSP-50 100g/L
Embodiment 33
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent N) and second reagent (reagent b).
First reagent (reagent N)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
エチレンジアミンPO40EO40 0.25g/L
Second reagent (reagent b)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
ユ ニ Le one Block MT-0620B 22.5g/L
Embodiment 34
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent Y) and second reagent (reagent b).
First reagent (reagent Y)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
COE-301 1kU/L
ニ ユ one コ one Le 1105-SN 2g/L
Second reagent (reagent b)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
ユ ニ Le one Block MT-0620B 22.5g/L
Embodiment 35
Preparation comprises following first reagent (reagent A E) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A E)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
パ one ソ Off ト EP 1.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 36
Preparation comprises following first reagent (reagent A F) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A F)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
パ one ソ Off ト EF 5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 37
Preparation comprises following first reagent (reagent A G) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A G)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
パ one ソ Off ト EFT 5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 38
Preparation comprises following first reagent (reagent A H) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A H)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
パ one ソ Off ト EDO 1.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 39
Preparation comprises following first reagent (reagent A I) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A I)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
ニ ユ one コ one Le 707-SF 5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Preparation comprises following first reagent (reagent A J) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A J)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
ニ ユ one コ one Le 707-SFC 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 41
Preparation comprises following first reagent (reagent A K) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A K)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
ニ ユ one コ one Le 723-SF 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 42
Preparation comprises following first reagent (reagent A L) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A L)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
エパンU108 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 43
Preparation comprises following first reagent (reagent A M) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A M)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
エパン420 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 44
Preparation comprises following first reagent (reagent A N) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A N)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
プルロニツクL-34 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 45
Preparation comprises following first reagent (reagent A O) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A O)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
プルロニツクF-68 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 46
Preparation comprises following first reagent (reagent A P) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A P)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
BLAUNON EP-1461 3g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 47
Preparation comprises following first reagent (reagent A Q) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A Q)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
エチレンジアミンPO40EO40 0.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 48
Preparation comprises following first reagent (reagent A R) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A R)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
ユ ニ セ one Off PKA-5015 3g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 49
Preparation comprises following first reagent (reagent A S) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A S)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.01kU/L of chemically modified
ユ ニ セ one Off PKA-5016 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Preparation comprises following first reagent (reagent A T) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A T)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
EMALEX DAPE-230 0.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 51
Preparation comprises following first reagent (reagent A U) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A U)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.01kU/L of chemically modified
BLAUNON BEO-10 0.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 52
Preparation comprises following first reagent (reagent A V) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A V)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
BLAUNON MXDA-P04 3g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 53
Preparation comprises following first reagent (reagent A W) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A W)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
ニ ユ one コ one Le B4-SN 0.05g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 54
Preparation comprises following first reagent (reagent A X) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A X)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
ニ ユ one コ one Le B13-SN 0.05g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kUg/L
プロノンB-208 20g/L
Embodiment 55
Preparation comprises following first reagent (reagent A Y) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A Y)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
ユ ニ セ one Off LM-2 1g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 56
Preparation comprises following first reagent (reagent A Z) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent A Z)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ノイゲンXL-400 0.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 57
Preparation comprises first following reagent (reagent BA) and second reagent (reagent RLP-C mensuration test kit a).
First reagent (reagent BA)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ノイゲンXL-1000F 0.5g/L
(reagent a) for second reagent
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
Embodiment 58
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent d).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent d)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 0.5kU/L
エマルゲンA-500 100g/L
Embodiment 59
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent e).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent e)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 0.5kU/L
ニ ユ one コ one Le 740 100g/L
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent f).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent f)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
EMALEX 2425 50g/L
Embodiment 61
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent g).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent g)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
ノイゲンTDS-500F 75g/L
Embodiment 62
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent F) and second reagent (reagent h).
First reagent (reagent F)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ニ ユ one カ Le ゲ Application GP-120 4g/L
Second reagent (reagent h)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プルロニツクF-108 75g/L
Embodiment 63
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BB) and second reagent (reagent i).
First reagent (reagent BB)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ノイゲンXL-400 0.75g/L
Second reagent (reagent i)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
エマルゲンA-500 75g/L
Embodiment 64
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BB) and second reagent (reagent j).
First reagent (reagent BB)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ノイゲンXL-400 0.75g/L
Second reagent (reagent j)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
ニ ユ one コ one Le 740 75g/L
Embodiment 65
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BB) and second reagent (reagent f).
First reagent (reagent BB)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 1kU/L
ノイゲンXL-400 0.75g/L
Second reagent (reagent f)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
EMALEX 2425 50g/L
Embodiment 66
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent A N) and second reagent (reagent k).
First reagent (reagent A N)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
プルロニツクL-34 1g/L
Second reagent (reagent k)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 0.5kU/L
エマルゲンA-500 50g/L
Embodiment 67
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent A N) and second reagent (reagent l).
First reagent (reagent A N)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
プルロニツクL-34 1g/L
Second reagent (reagent l)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 0.5kU/L
ニ ユ one コ one Le 740 50g/L
Embodiment 68
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent A N) and second reagent (reagent m).
First reagent (reagent A N)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
プルロニツクL-34 1g/L
Second reagent (reagent m)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
ノイゲンTDS-500F 50g/L
Embodiment 69
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BC) and second reagent (reagent n).
First reagent (reagent BC)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
BIGCHAP 0.5g/L
Second reagent (reagent n)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 2g/L
Embodiment 70
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BD) and second reagent (reagent n).
First reagent (reagent BD)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
deoxy-BIGCHAP 0.5g/L
Second reagent (reagent n)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 2g/L
Embodiment 71
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BE) and second reagent (reagent n).
First reagent (reagent BE)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
CHAPSO 0.5g/L
Second reagent (reagent n)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 2g/L
Embodiment 72
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BD) and second reagent (reagent o).
First reagent (reagent BD)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
deoxy-BIGCHAP 0.5g/L
Second reagent (reagent o)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
ユ ニ Le one Block MT-0620B 2g/L
Embodiment 73
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BD) and second reagent (reagent p).
First reagent (reagent BD)
MOPS(pH7.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
The LPL 0.025kU/L of chemically modified
deoxy-BIGCHAP 0.5g/L
Second reagent (reagent p)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
CEN 20kU/L
エマルゲンA-500 40g/L
Embodiment 74
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BF) and second reagent (reagent q).
First reagent (reagent BF)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
BIGCHAP 0.5g/L
Second reagent (reagent q)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
CEN 20kU/L
プロノンB-208 2g/L
Embodiment 75
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BF) and second reagent (reagent p).
First reagent (reagent BF)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
BIGCHAP 0.5g/L
Second reagent (reagent p)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
CEN 20kU/L
エマルゲンA-500 40g/L
Embodiment 76
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent BF) and second reagent (reagent r).
First reagent (reagent BF)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 0.75g/L
The LPL 0.05kU/L of chemically modified
BIGCHAP 0.5g/L
Second reagent (reagent r)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
CEN 20kU/L
EMALEX 2425 2g/L
Embodiment 77
Use the RLP-C of record in the reference example 1 to measure the test kit of using test kit (hereinafter referred to as the contrast agents box) and embodiment 2～4, measure the RLP-C concentration in 45 corpse or other object for laboratory examination and chemical testing of human serum.In addition, reference example 2 is the properties data that show the contrast agents box of reference example 1 record.
(1) makes working curve
Use physiological saline (RLP-C:0.0mg/dL) and by use RLP-cholesterol " JIMRO " II (Japanese antibody institute company manufacturing) to measure to determine RLP-C concentration as the standard serum of 35.0mg/dL as reference liquid, use the test kit of contrast agents box and embodiment 2～4, by the automatic analytical engine of Hitachi's 7170S type, for each test kit, make the working curve of expression RLP-C and " absorbancy " relation.
" absorbancy " described herein expression is based on 2 absorbancys (E1 and E2) of measuring in the following reaction, by deduct the value that E1 obtains from E2.
In reaction tank, add reference liquid (3.2 μ L) and first reagent (150 μ L), 37 ℃ of heating 5 minutes, measure the absorbancy (E1) of this reaction solution at predominant wavelength 600nm, commplementary wave length 700nm place, then, in this reaction solution, add second reagent (50 μ L), further 37 ℃ of heating 5 minutes, then in the absorbancy (E2) of predominant wavelength 600nm, commplementary wave length 700nm place assaying reaction liquid.
(2) " absorbancy " of a human serum corpse or other object for laboratory examination and chemical testing and this corpse or other object for laboratory examination and chemical testing measured
In the working curve of (1) was made, end user's serum corpse or other object for laboratory examination and chemical testing (45 corpse or other object for laboratory examination and chemical testing) replaced reference liquid, in addition, carried out the same operation of " absorbancy " method of calculation with (1), measured " absorbancy " of this corpse or other object for laboratory examination and chemical testing.
(3) the RLP-C concentration in the human serum corpse or other object for laboratory examination and chemical testing determines
By the working curve of making in " absorbancy " and (1) measured in (2), measure the RLP-C concentration in each corpse or other object for laboratory examination and chemical testing.
RLP-C concentration in each corpse or other object for laboratory examination and chemical testing that obtains by using the contrast agents box to measure and use the test kit of embodiment 2～4 to measure and RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of obtaining is obtained the relation conefficient of the mensuration of the test kit that uses contrast agents box and embodiment 2～4.The result is as shown in table 1.
Table 1
| Test kit | Relation conefficient with respect to the contrast agents box |
| Embodiment 2 | 0.8505 |
| Embodiment 3 | 0.8808 |
| Embodiment 4 | 0.8447 |
As shown in table 1, between the mensuration of using the contrast agents box and the mensuration of the test kit that uses embodiment 2～4 relevant well.
Embodiment 78
The RLP-C that uses company of Japanese antibody institute to make measures with test kit (goods name: RLP-cholesterol " JIMRO " II), carry out the operation of appended specification sheets record, measure the RLP-C concentration of 40 corpse or other object for laboratory examination and chemical testing of a Freshman serum corpse or other object for laboratory examination and chemical testing.In addition, use the test kit of contrast agents box and embodiment 1,5～11,14～30, by measuring RLP-C concentration in these 40 corpse or other object for laboratory examination and chemical testing with the same method of embodiment 77.By the RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of this 40 corpse or other object for laboratory examination and chemical testing of the kit measurement that uses contrast agents box and embodiment 1,5～11,14～30 and use RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of these 40 corpse or other object for laboratory examination and chemical testing that RLP-cholesterol " JIMRO " II measures, obtain the relation conefficient of the test kit of RLP-cholesterol " JIMRO " II and embodiment 1,5～11,14～30.The result is as shown in table 2.
Table 2
| Test kit | Relation conefficient with respect to JIMRO |
| The contrast agents box | 0.9638 |
| Embodiment 1 | 0.9341 |
| Embodiment 5 | 0.9210 |
| Embodiment 6 | 0.9454 |
| Embodiment 7 | 0.9379 |
| Embodiment 8 | 0.8896 |
| Embodiment 9 | 0.9183 |
| |
0.8990 |
| Embodiment 11 | 0.8179 |
| Embodiment 14 | 0.9436 |
| Embodiment 15 | 0.9281 |
| Embodiment 16 | 0.9013 |
| Embodiment 17 | 0.8447 |
| Embodiment 18 | 0.9546 |
| Embodiment 19 | 0.9384 |
| |
0.8635 |
| Embodiment 21 | 0.9271 |
| Embodiment 22 | 0.9598 |
| Embodiment 23 | 0.9224 |
| Embodiment 24 | 0.8721 |
| Embodiment 25 | 0.9280 |
| Embodiment 26 | 0.9209 |
| Embodiment 27 | 0.9107 |
| Embodiment 28 | 0.9464 |
| Embodiment 29 | 0.8663 |
| |
0.9244 |
As shown in table 2, use the test kit of contrast agents box and embodiment 1,5～11,14～30 mensuration, with the mensuration of using RLP-cholesterol " JIMRO " II between relevant well.
Embodiment 79
Use the test kit of contrast agents box and embodiment 1,5～76, by measuring RLP-C concentration in 40 corpse or other object for laboratory examination and chemical testing of a human serum corpse or other object for laboratory examination and chemical testing with the same method of embodiment 77.By the RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of this 40 corpse or other object for laboratory examination and chemical testing of the kit measurement that uses embodiment 1,5～76, and use RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of these 40 corpse or other object for laboratory examination and chemical testing that the contrast agents box measures, obtain the relation conefficient of the test kit of contrast agents box and embodiment 1,5～76.The result is as shown in table 3.
Table 3
As shown in table 3, between the mensuration of using the contrast agents box and the mensuration of the test kit that uses embodiment 1,5～76 relevant well.
Embodiment 80
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent s).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent s)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
PLDP 5kU/L
Embodiment 81
Preparation comprises the RLP-C mensuration test kit of following first reagent (reagent E) and second reagent (reagent t).
First reagent (reagent E)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Sodium sulfate 1g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
COE-301 2kU/L
エマルゲンB-66 2.5g/L
Second reagent (reagent t)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
PLA2 3kU/L
Embodiment 82
Preparation comprises the RLP-C mensuration test kit of first following reagent (reagent W) and second reagent (reagent s).
First reagent (reagent W)
MOPS(pH6.5) 4g/L
EMSE 0.3g/L
Peroxidase 10kU/L
CHO-CE 2kU/L
Dextran sulfate sodium 1.5g/L
Magnesium nitrate hexahydrate 1.5g/L
COE-301 1kU/L
ニ ユ one コ one Le 707-SN 4g/L
Second reagent (reagent s)
MOPS(pH6.8) 4g/L
4-aminoantipyrene 0.5g/L
Peroxidase 20kU/L
CHO-CE 2kU/L
LPL-312 1.5kU/L
プロノンB-208 20g/L
PLDP 5kU/L
Embodiment 83
Use the test kit of contrast agents box and embodiment 5,23,80～82,, utilize the automatic analytical engine of Hitachi's 7170S type to measure RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of 50 corpse or other object for laboratory examination and chemical testing of human serum by the method same with embodiment 77.By the RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of these 50 corpse or other object for laboratory examination and chemical testing that use the contrast agents box to measure and use RLP-C concentration in each corpse or other object for laboratory examination and chemical testing of this 50 corpse or other object for laboratory examination and chemical testing of kit measurement of embodiment 5,23,80～82, obtain the relation conefficient of the test kit of contrast agents box and embodiment 5,23,80～82.The result is as shown in table 4.
Table 4
| Test kit | The phosphatide lytic enzyme | Relation conefficient with respect to the contrast agents box |
| Embodiment 5 | - | 0.9054 |
| Embodiment 80 | PLDP | 0.9505 |
| Embodiment 81 | PLA2 | 0.9229 |
| Embodiment 23 | - | 0.8583 |
| Embodiment 82 | PLDP | 0.9219 |
According to table 4, from the comparison of the mensuration of the test kit that uses embodiment 5 and the mensuration of the test kit that uses embodiment 80,81 and the comparison of mensuration and the mensuration of the test kit that uses embodiment 82 of using the test kit of embodiment 23, as can be known by in second reagent, appending the phosphatide lytic enzyme, with the relation conefficient raising in the mensuration of using the contrast agents box relevant.
Reference example 1
Making comprises the RLP-C reagents for quantitatively box of following first reagent and second reagent.
First reagent
MOPS(pH6.8) 20mmol/L
TOOS 0.3g/L
Sodium sulfate 2g/L
プロノンB-208 8g/L
ワ Application グ サ one Off RL-100 1g/L
Peroxidase 10kU/L
Vitamin C oxidase 2kU/L
Second reagent
MOPS(pH6.8) 20mmol/L
4-aminoantipyrene 0.5g/L
ワ Application グ サ one Off RL-100 1g/L
Peroxidase 10kU/L
Sterol esterase (Japan's textile company is made) 1kU/L
RCO (consonance Kyowa Hakko makes) 3kU/L
Phospholipid hydrolase (manufacturing of company of Asahi Chemical Industry) 5kU/L
Reference example 2RLP-C's is quantitative
Use the test kit of reference example 1,, utilize Hitachi's automatic analytical engine of 7170 types by the following method the RLP-C in each corpse or other object for laboratory examination and chemical testing to be carried out quantitatively for 64 corpse or other object for laboratory examination and chemical testing of people's fresh serum.
(1) makes working curve
Use by uses RLP-cholesterol " JIMRO " II (Japanese antibody institute company manufacturing) to measure to determine RLP-C concentration as the serum reference liquid of 32.4mg/dL as standard substance, with the dilute serum that suitably dilutes this serum reference liquid and prepare as working curve making sample.
Use the test kit of reference example 1 use test kit,, utilize Hitachi's automatic analytical engine of 7170 types to measure RLP-C concentration in each dilute serum, making working curve according to following order as measuring.
In reaction tank, add dilute serum (3.8 μ L) and first reagent (0.18mL), 37 ℃ of heating 5 minutes, measure (photometry point: the 16) absorbancy of reaction solution (E1) at predominant wavelength 600nm, commplementary wave length 700nm place, then, in this reaction solution, add second reagent (0.06mL) that is heated to 37 ℃ in advance, further, measure (photometry point: the 32) absorbancy of reaction solution (E2) at predominant wavelength 600nm, commplementary wave length 700nm place then 37 ℃ of heating 5 minutes.The relation that is changed the RLP-C concentration in (E2-E1) and the dilute serum by the absorbancy in the reaction is made working curve.
(2) RLP-C's in 64 corpse or other object for laboratory examination and chemical testing of people's fresh serum is quantitative
64 corpse or other object for laboratory examination and chemical testing substitute blood serum of end user's fresh serum reference liquid by the method same with (1), reacts respectively these 64 corpse or other object for laboratory examination and chemical testing, and the working curve by the absorbancy (1) of reacted reaction solution is made carries out quantitatively the RLP-C concentration in each corpse or other object for laboratory examination and chemical testing.
(3) relevant with the mensuration of using RLP-cholesterol " JIMRO " II (Japanese antibody institute company make)
For 64 corpse or other object for laboratory examination and chemical testing of aforesaid people's fresh serum, use RLP-cholesterol " JIMRO " II (Japanese antibody institute company make) that the RLP-C concentration in each corpse or other object for laboratory examination and chemical testing is carried out quantitatively.
For each corpse or other object for laboratory examination and chemical testing, estimate the dependency of the quantitative values of above-mentioned (2) and (3) with relation conefficient.As a result, relation conefficient is 0.9513.In addition, correlogram as shown in Figure 1.
Industrial applicability
According to the present invention, assay method to the cholesterol in the effective remnant-like lipoprotein of diagnosis of artery sclerosis etc. is provided, measures with reagent and mensuration kit.
Claims (27)
1. the method for the cholesterol (being designated hereinafter simply as RLP-C) in the remnant-like lipoprotein (being designated hereinafter simply as RLP) of measuring in the corpse or other object for laboratory examination and chemical testing is characterized in that, carries out following steps (i)～(iii) successively:
(i) containing a corpse or other object for laboratory examination and chemical testing, and be selected from by the polyethylene oxide alkyl ethers phosphoric acid ester, the polyethylene oxide alkyl ethers sulfuric acid, higher alcohol sulfate salt, the HLB value is encircled phenyl ether less than 16.0 polyoxyethylene more, polyoxyethylene encircles the phenyl ether sulfuric acid more, polyoxyethylene polyoxygenated alkene encircles phenyl ether more, quadrol-polyoxyethylene polyoxytrimethylene condenses, the polyoxyethylene alkyl phenyl ether, polyoxyethylene alkyl phenyl ether sulfuric acid, polyoxyethylene short-chain branches alkyl oxide, the molecular weight of polyoxytrimethylene is less than 2750 polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene polyoxygenated alkene short-chain branches alkyl oxide, polyoxyethylene polyoxygenated alkene alkyl oxide, bile acid derivative, the polyoxyethylene Bisphenol A Ether, in the aqueous medium of the tensio-active agent in the group that polyoxyethylene aryl ethers sulfuric acid and polyoxytrimethylene m-xylene diamine are formed (following abbreviate this tensio-active agent as surfactant A), make a corpse or other object for laboratory examination and chemical testing and rCO, perhaps cholesterol dehydrogenase and oxidized coenzyme react, and remove the step of the cholesterol in the RLP lipoprotein in addition;
(ii) make to have removed to exist in the reaction solution after the cholesterol in the lipoprotein beyond the RLP and be selected from by polyoxyethylene polyoxybutylene condenses by above-mentioned steps (i), the HLB value is that the polyoxyethylene more than 16.0 encircles phenyl ether more, polyoxyethylene polyoxygenated alkene long-chain branched-chain alkyl ether, the molecular weight of polyoxytrimethylene be in the group formed of polyoxyethylene polyoxytrimethylene condenses more than 2750 and polyoxyethylene long-chain branched-chain alkyl ether tensio-active agent (below, abbreviate this tensio-active agent as surfactant B), make cholesterol ester hydrolase and rCO, perhaps cholesterol ester hydrolase, cholesterol dehydrogenase and oxidized coenzyme reaction, the step of generation hydrogen peroxide or reduced coenzyme; And
(iii) measure the (ii) middle hydrogen peroxide that generates of above-mentioned steps or the step of reduced coenzyme.
2. the method for claim 1, wherein, step (i) is in the aqueous medium that contains a corpse or other object for laboratory examination and chemical testing and surfactant A, makes the reaction of cholesterol dehydrogenase, oxidized coenzyme and reduced coenzyme oxydase and a corpse or other object for laboratory examination and chemical testing, removes the step of the cholesterol in the RLP lipoprotein in addition.
3. method as claimed in claim 1 or 2, wherein, being reflected under the polyanion existence of step (i) carried out.
4. method as claimed in claim 3, wherein, surfactant A is to be selected from tensio-active agent in the group of being made up of less than 2750 polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene polyoxygenated alkene short-chain branches alkyl oxide and bile acid derivative the molecular weight of quadrol-polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene alkyl phenyl ether, polyoxyethylene alkyl phenyl ether sulfuric acid, polyoxyethylene short-chain branches alkyl oxide, polyoxytrimethylene [below, abbreviate this tensio-active agent as tensio-active agent (A1)].
5. method as claimed in claim 1 or 2, wherein, the metal ion existence that is reflected at polyanion and divalence of step (i) is carried out down or in the presence of the antibody in being selected from the group of being made up of anti-apolipoprotein B antibody and anti-apo E antibody.
6. method as claimed in claim 5, wherein, surfactant A is for being selected from by the polyethylene oxide alkyl ethers sulfuric acid, polyoxyethylene encircles the phenyl ether sulfuric acid more, polyoxyethylene polyoxygenated alkene encircles phenyl ether more, quadrol-polyoxyethylene polyoxytrimethylene condenses, the polyoxyethylene alkyl phenyl ether, the molecular weight of polyoxytrimethylene is less than 2750 polyoxyethylene polyoxytrimethylene condenses, polyoxyethylene polyoxygenated alkene short-chain branches alkyl oxide, tensio-active agent in the group that polyoxyethylene polyoxygenated alkene alkyl oxide and bile acid derivative are formed [below, abbreviate this tensio-active agent as tensio-active agent (A2)].
7. as each described method in the claim 1～6, wherein, step (i) is carried out under the coexistence of cholesterol ester hydrolase.
8. as each described method in the claim 1～7, wherein, step is (ii) carried out under the coexistence of phosphatide lytic enzyme.
9. as each described method in the claim 1～8, wherein, the elimination of hydrogen peroxide is in the presence of the combination of peroxidase and one of two kinds of oxidative coupling type chromogens that use in oxidative coupling color development reaction or carry out in the presence of catalatic.
10. reagent that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it contains cholesterol ester hydrolase, rCO, hydrogen peroxide removes reagent, hydrogen peroxide assay reagents, surfactant A and surfactant B.
11. a reagent that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it contains cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme, surfactant A and surfactant B.
12. a reagent that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it contains cholesterol ester hydrolase, cholesterol dehydrogenase, oxidized coenzyme, reduced coenzyme oxydase, hydrogen peroxide removes reagent, hydrogen peroxide assay reagents, surfactant A and surfactant B.
13. as claim 10 or 12 described reagent, wherein, it is to contain peroxidase and the reagent of one of two kinds of oxidative coupling type chromogens using in oxidative coupling color development reaction or contain catalatic reagent that hydrogen peroxide is removed reagent.
14. as each described reagent in the claim 10～13, it further contains polyanion.
15. reagent as claimed in claim 14, wherein, surfactant A is tensio-active agent (A1).
16. as each described reagent in the claim 10～13, it further contains: contain the reagent of polyanion and divalent-metal ion or contain the reagent that is selected from the antibody in the group of forming by anti-apolipoprotein B antibody and anti-apo E antibody.
17. reagent as claimed in claim 16, wherein, surfactant A is tensio-active agent (A2).
18. as each described reagent in the claim 10～17, it further contains the phosphatide lytic enzyme.
19. test kit that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it is characterized in that, contain first reagent and second reagent, described first reagent contains rCO, hydrogen peroxide is removed reagent and surfactant A, and described second reagent contains hydrogen peroxide assay reagents and surfactant B; And make among any one or two in first reagent and second reagent and contain cholesterol ester hydrolase.
20. a test kit that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing is characterized in that contain first reagent and second reagent, described first reagent contains cholesterol dehydrogenase, oxidized coenzyme and surfactant A, described second reagent contains surfactant B; And make among any one or two in first reagent and second reagent and contain cholesterol ester hydrolase.
21. test kit that is used for measuring the RLP-C of a corpse or other object for laboratory examination and chemical testing, it is characterized in that, contain first reagent and second reagent, described first reagent contains cholesterol dehydrogenase, oxidized coenzyme, reduced coenzyme oxydase, hydrogen peroxide removes reagent and surfactant A, and described second reagent contains hydrogen peroxide assay reagents and surfactant B; And make among any one or two in first reagent and second reagent and contain cholesterol ester hydrolase.
22. as claim 19 or 21 described test kits, wherein, it is to contain peroxidase and the reagent of one of two kinds of oxidative coupling type chromogens using in oxidative coupling color development reaction or contain catalatic reagent that hydrogen peroxide is removed reagent.
23. as each described test kit in the claim 19～22, wherein, first reagent further contains polyanion.
24. test kit as claimed in claim 23, wherein, surfactant A is tensio-active agent (A1).
25. as each described test kit in the claim 19～22, wherein, first reagent further contains the metal ion of polyanion and divalence or is selected from antibody in the group of being made up of anti-apolipoprotein B antibody and anti-apo E antibody.
26. test kit as claimed in claim 25, wherein, surfactant A is tensio-active agent (A2).
27. as each described test kit in the claim 19～26, wherein, second reagent further contains the phosphatide lytic enzyme.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP356586/2005 | 2005-12-09 | ||
| JP2005356586 | 2005-12-09 | ||
| JP095152/2006 | 2006-03-30 |
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|---|---|
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314113A (en) * | 2010-11-10 | 2013-09-18 | 电化生研株式会社 | Method for quantification of remnant-like lipoprotein cholesterol and kit for same |
| CN109312389A (en) * | 2016-05-24 | 2019-02-05 | 电化生研株式会社 | The quantitative approach and quantitative reagent of cholesterol in lipoprotein rich in triglycerides |
-
2006
- 2006-12-08 CN CNA2006800464391A patent/CN101326289A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314113A (en) * | 2010-11-10 | 2013-09-18 | 电化生研株式会社 | Method for quantification of remnant-like lipoprotein cholesterol and kit for same |
| CN104894220A (en) * | 2010-11-10 | 2015-09-09 | 电化生研株式会社 | Method for quantification of remnant-like lipoprotein cholesterol and kit for same |
| CN103314113B (en) * | 2010-11-10 | 2015-11-25 | 电化生研株式会社 | The cholesteric quantivative approach of remnant-like lipoprotein and for its test kit |
| CN104894220B (en) * | 2010-11-10 | 2018-06-08 | 电化生研株式会社 | The cholesteric quantitative approach of remnant-like lipoprotein and the kit for it |
| CN109312389A (en) * | 2016-05-24 | 2019-02-05 | 电化生研株式会社 | The quantitative approach and quantitative reagent of cholesterol in lipoprotein rich in triglycerides |
| CN109312389B (en) * | 2016-05-24 | 2023-09-19 | 电化株式会社 | Method for quantifying cholesterol in triglyceride-rich lipoprotein and quantification reagent |
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