CN101325881A - Equol-containing fermentation product of soybean embryonic axis, and method for production thereof - Google Patents

Abstract

Disclosed is a fermentation product of a soybean embryonic axis which contains equol and is useful as a material for a food, pharmaceutical, cosmetic or the like. The fermentation product can be produced by fermenting a soybean embryonic axis with a microorganism capable of assimilating at least one daidzein-type substance selected from the group consisting of a glycoside of daidzein, daidzein and dihydrodaidzein to produce equol.

Description

Contain soybean plumular axis fermentate of equol and preparation method thereof

Technical field

The present invention relates to contain soybean plumular axis (soybean embryonicaxis) fermentate of equol (equol) and preparation method thereof.

Background technology

Contained isoflavones (isoflavones in the soybean; Daidzein (daidzein), genistein (genistein), Glycitein (glycitein)) similar with the estradiol structure, have the estrogenic antagonist and the estrogen-like action that produce along with combining of ERs (following table is shown ER).Up to now, the epidemiological study of isoflavones or get involved the effect of improving that research has been pointed out the preventive effect of hormonal dependent cancers such as the breast cancer of utilizing estrogenic antagonist to obtain, prostate cancer or utilized the climacteric obstacle that estrogen-like action obtains, postclimacteric osteoporosis, hyperlipemia.

In recent years, the active body of having pointed out the physiological action of above-mentioned isoflavones may be the metabolin equol of daidzein.That is, reported with isoflavones and compared that equol is strong with the binding ability (particularly combining with ER β) of ER, the dividing a word with a hyphen at the end of a line property that enters target internal organs such as breast or prostata tissue is very high.It is significantly few to have reported also in addition in patient-comparative study in breast cancer, the patients with prostate cancer that equol produces the survivor, the improve effect of isoflavones to postclimacteric bone density, lipid-metabolism is divided into equol and produces survivor and non-and produce the survivor when resolving, and equol produces the survivor and significantly is enhanced.

Equol is produced through the metabolism of intestinal bacterium by daidzein, but equol generation ability has individual difference, has reported that the ratio that the equol among the Japanese produces the survivor is about 50%.That is to say that having an appointment among the Japanese 50% can not produce equol (the non-product of equol survivor), in these people, even picked-up soybean or soybean processed foods also can't be enjoyed the useful physiologic effect based on the equol effect.Therefore, think that it is effective making its picked-up equol itself in order to make the non-product of equol survivor present useful physiologic effect based on the equol effect.

At present, as the preparation method of equol, the microorganism (following table is shown equol and produces bacterium) of known useful assimilation daidzein generation equol carries out the method for fermentation process to the raw material that contains daidzein.In this preparation method, as the used raw material that contains daidzein, known have soybean, root of kudzu vine soup, red earth grape (red globe grape), a clover etc.In addition, it also has been known that equol produces bacterium, for example, the inventor waits and isolated bacteroid E-23-15 (FERM BP-6435 number), streptococcus E-23-17 (FERM BP-6436 number), streptococcus A6G225 (FERM BP-6437 number) and galactococcus 20-92 (FERM BP-10036 number) (referring to patent documentation 1 and 2) from human feces.

But, also there is following problems: use equol to produce bacterium simply the raw material that contains above-mentioned daidzein class is carried out fermentation process, equol amount in the gained fermentate is insufficient, even directly absorb this fermentate, can not fully expect desirable useful effect based on the equol effect.

On the other hand, knownly partly compare with the cotyledon that is used as soybean processed foods, the soybean plumular axis part to be containing useful components such as isoflavones or saponin at high proportion, and its extract has been developed various uses (for example patent documentation 3).But the soybean plumular axis extract itself has the high shortcoming of cost.In addition, when existing in the soybean plumular axis abstract, in order to produce the problem that bacterium is fermented and must add nutrient in addition with equol as the raw material of preparation equol.Above-mentioned reason causes the soybean plumular axis abstract can't be used as raw material at present when industrial preparation equol.

On the other hand, because soybean plumular axis itself has distinctive bitter taste, so have the tendency of avoiding directly utilizing this soybean plumular axis itself, most of soybean plumular axis is dropped.In addition, soybean plumular axis is the same with the cotyledon part of soybean, contains the anaphylactogen material, so concerning the people with soybean allergy, can't absorb or give soybean plumular axis.Therefore, in order effectively to utilize soybean plumular axis, to improve its serviceability be important by making soybean plumular axis itself further have surcharge.

Patent documentation 1: the international specification that discloses No. 99/007392

Patent documentation 2: the international specification that discloses No. 2005/000042

Patent documentation 3: the spy opens the 2002-234844 communique

Summary of the invention

The object of the present invention is to provide contain equol as useful soybean plumular axis fermentates such as raw-food material, pharmaceuticals raw material or cosmetic materials.And then, the object of the present invention is to provide preparation to contain the method for the soybean plumular axis fermentate of equol.

The inventor etc. have carried out deep research in order to solve above-mentioned problem, found that and use the microorganism of ability to make the soybean plumular axis fermentation with assimilation daidzein class generation equol, can generate equol with high efficiency, obtain containing the soybean plumular axis fermentate of equol.And then find, owing in the soybean plumular axis fermentate that contains equol of gained thus, reduced the contained anaphylactogen of soybean plumular axis, so also be useful as the raw material of low-allergen.Based on above-mentioned cognition, improve repeatedly, thereby finished the present invention.

That is, the invention provides the invention that relates to following soybean plumular axis fermentate that contains equol and uses thereof:

1. 1 kinds of soybean plumular axis fermentates that contain equol, with have assimilation be selected from daidzein glucosides (daidzein glycosides), daidzein, and DIHYDRADAIDZEIN at least a daidzein class microorganism that produces the ability of equol the soybean plumular axis fermentation is obtained.

An item 2. as 1 a described soybean plumular axis fermentate, wherein, described microorganism is the lactic acid bacteria that belongs to lactococcus.

3. as 1 a described soybean plumular axis fermentate, wherein, described microorganism be the Ge Shi galactococcus ( Lactococcus garvieae).

An item 4. as 1 a described soybean plumular axis fermentate, wherein, the soybean plumular axis fermentate of every dry weight contains 0.1～20 weight % equol.

An item 5. as 1 a described soybean plumular axis fermentate, wherein, described soybean plumular axis fermentate also contains daidzin (daidzin) class, genistin (genistin) class, genistein class, soya bean xanthosine (glycitin) class and Glycitein class.

An item 6. as 1 a described soybean plumular axis fermentate wherein, also contain ornithine.

7. 1 kinds of food of item contain 1 a described soybean plumular axis fermentate.

An item 8. as 7 a described food are dietary supplements (dietary supplement).

An item 9. as 7 a described food, wherein, every 100g food contains the described soybean plumular axis fermentate of 0.1～90g.

10. 1 kinds of pharmaceutical preparations of item contain 1 a described soybean plumular axis fermentate.

11. as 10 a described pharmaceutical preparation, be climacteric obstacle, osteoporosis, hypertrophy of the prostate or the prevention or the therapeutic agent of metabolic syndrome.

An item 12. as 10 a described pharmaceutical preparation are depressants of cholesterine value in the blood.

13. 1 described soybean plumular axis fermentates of item are in preparation climacteric obstacle, osteoporosis, hypertrophy of the prostate or the prevention of metabolic syndrome or the purposes in the therapeutic agent.

Purposes in the depressant of 14. 1 described soybean plumular axis fermentates of item cholesterine value in preparation blood.

15. 1 kinds climacteric obstacle methods of treatment, it is characterized in that the patient of obstacle gives the item 1 described soybean plumular axis fermentate of effective dose to climacteric.

The reduction method of cholesterine value is characterized in that in 16. 1 kinds of blood of item, the patient of cholesterine value in the needs reduction blood is given the item 1 described soybean plumular axis fermentate of effective dose.

17. 1 kinds of cosmetics of item contain 1 a described soybean plumular axis fermentate.

An item 18. as 17 a described cosmetics, wherein, every 100g cosmetics contain the described soybean plumular axis fermentate of 0.1～10g.

19. 1 kinds of preparation methods that contain the soybean plumular axis fermentate of equol of item, it is characterized in that, assimilate the microbial fermentation processing soybean plumular axis that at least a daidzein class that is selected from daidzein glucosides, daidzein and the DIHYDRADAIDZEIN produces the ability of equol with having.

Soybean plumular axis fermentate of the present invention is owing to containing equol and useful components such as isoflavones except that equol or saponin, so be useful in fields such as food, pharmaceuticals, cosmetics.Soybean plumular axis fermentate particularly of the present invention is compared with the fermentate that makes soybean, root of kudzu vine soup, red earth grape, clover etc. contain the fermenting raw materials gained of daidzein, the growing amount of equol is many especially, can further bring into play the useful physiologic effect based on equol well.

And then, because soybean plumular axis fermentate of the present invention has reduced anaphylactogen contained in the soybean plumular axis, so as the hypoallergenic raw material, even have the advantage that the people to soybean allergy also can absorb safely or be suitable for.In addition, it is raw material that soybean plumular axis fermentate of the present invention adds the soybean plumular axis that is dropped man-hour with soy food product, from the viewpoint of efficent use of resources, and the value height on the industry.

Description of drawings

[Fig. 1] is the figure of the equol concentration in the zymotic fluid of gained among the expression embodiment 1-3.

[Fig. 2] is the figure that expression detects result's (electrophoretogram) of gross protein contained in soybean plumular axis fermentate, soybean cotyledon and the soybean plumular axis of gained among the embodiment 1.

[Fig. 3] is result's's (electrophoretogram) of the contained main anaphylactogen (Gym4, Gm30K, Gm28K) of soybean plumular axis fermentate, soybean cotyledon and the soybean plumular axis of gained among the expression detection embodiment 1 figure.

Result's's (electrophoretogram) of contained main anaphylactogen (7S globulin mix, oleosin (oleosin), trypsin inhibitor) figure in soybean plumular axis fermentate, soybean cotyledon and the soybean plumular axis of gained among [Fig. 4] expression embodiment 1.

The specific embodiment

Below, describe the embodiment that the present invention is specialized in detail.

Among the present invention, produce bacterium, use to have the microorganism that assimilation is selected from the ability (metabolic activity) of at least a daidzein class generation equol in daidzein glucosides, daidzein and the DIHYDRADAIDZEIN as equol.As the daidzein glucosides, can enumerate daidzin, malonyl daidzin, acetyl daidzin etc. particularly herein.

As mentioned microorganism (equol produce bacterium),, be not particularly limited for example known microorganism that existence has aforementioned capabilities in following microorganism as long as allow on the food hygiene and have aforementioned capabilities: the Ge Shi galactococcus ( Lactococcus garvieae) wait the microorganism that belongs to lactococcus; The intermediate chain coccus ( Strertococcus intermedius), streptococcus anginosus ( Streptococcus constellatus) wait the microorganism that belongs to streptococcus; Avette pseudobacillus ( Bacteroides ovatus) wait the microorganism that belongs to Bacteroides.Produce in the bacterium at equol, be preferably the lactic acid bacteria of lactococcus and streptococcus etc., more preferably belong to the lactic acid bacteria of lactococcus, especially preferably enumerate the Ge Shi galactococcus.Microorganism with aforementioned capabilities can be an index to have or not equol generation ability for example, separates from human faecal mass.Produce bacterium for above-mentioned equol, preservation wait from human feces by inventor and separate the bacterium of identifying, be galactococcus 20-92 (FERM BP-10036 number), streptococcus E-23-17 (FERM BP-6436 number), streptococcus A6G225 (FERM BP-6437 number) and bacteroid E-23-15 (FERMBP-6435 number), can use above-mentioned preservation bacterium among the present invention.The preferred galactococcus 20-92 that uses in the above-mentioned preservation bacterium.

Among the present invention, fermentation raw material uses soybean plumular axis.Form the part of young shoot, young root when known soybean plumular axis is soybean germination, contain daidzein classes such as a large amount of daidzein glucosides or daidzein.Be used for soybean plumular axis of the present invention and be limited to contained daidzein class and do not have the soybean plumular axis that runs off, to the place of production of soybean or have or not processing unqualified.It for example can be unprocessed soybean plumular axis; The soybean plumular axis of from soybean, separating through heat treated, dry processing, boiling processing etc.; Carry out heat treated, the dry processing or soybean plumular axis that boiling processing etc. obtains etc. to separate the plumular axis that obtains from unprocessed soybean.In addition, being used for soybean plumular axis of the present invention can be the soybean plumular axis that process ungrease treatment or deproteinization are handled.The shape that is used for soybean plumular axis of the present invention is not particularly limited, can be for Powdered, also can be for being pulverized or broken soybean plumular axis.From more effectively generating the viewpoint of equol, preferably use Powdered soybean plumular axis.

The fermentation process of soybean plumular axis is regulated moisture by add suitable quantity of water in soybean plumular axis, above-mentioned equol generation bacterium is inoculated in wherein carries out.

The amount that is added into the water in the soybean plumular axis produces the kind of bacterium or the kind of fermentation tank etc. and suitably setting according to used equol.Usually, when beginning to ferment, soybean plumular axis and water get final product in following ratio coexistence: with respect to 100 weight portion soybean plumular axis (being converted into dry weight), water is 400～4000 weight portions, be preferably 500～2000 weight portions, more preferably 600～1000 weight portions.

In addition, in the fermentation of soybean plumular axis,, can in soybean plumular axis, add nitrogenous sources such as yeast extract, poly-peptone, meat broth as required as fermentation raw material in order to promote fermentation efficiency or to increase the taste etc. of fermentate; Carbon source such as glucose, sucrose; Inorganic salts such as phosphate, carbonate, sulfate; Vitamins; Nutritional labelings such as amino acid.Particularly, producing bacterium as equol uses and has the equol that arginine is converted into the ability of ornithine when producing bacterium (following table is shown " the ornithine equol produces bacterium "), in soybean plumular axis, add arginine and ferment, can make thus in the fermentate of gained and contain ornithine.At this moment, arginic addition for example can be enumerated with respect to 100 weight portion soybean plumular axis (being converted into dry weight), and arginine is about 0.5～3 weight portion.Need to prove,, can be selected from the form galactococcus particularly, particularly, can enumerate galactococcus 20-92 (FERM BP-10036 number) as having the equol generation bacterium that arginine is converted into the ability of ornithine.

And then, used fermentation raw material (soybean plumular axis contains thing) if pH can cultivate equol and produce bacterium, be not particularly limited, produce the viewpoint that bacterium well breeds from making equol, preferred in advance the pH of fermentation raw material is adjusted to about 6～7, is preferably about 6.3～6.8.

In addition, can also add the isoflavones that contains above-mentioned daidzein class in the fermentation raw material of use (soybean plumular axis contains thing).Thus by in fermentation raw material, adding isoflavones in advance in addition, can further improve the equol content in the soybean plumular axis fermentate of gained, thereby further improve its serviceability.

The fermentation of soybean plumular axis is carried out under the environmental condition of the cultivation characteristic that satisfies the equol generation bacterium of using.For example, when using the above-mentioned equol of specifically enumerating to produce bacterium, the fermentation of soybean plumular axis is carried out under the anaerobic condition.

In addition,, get final product, for example can enumerate 20～40 ℃, preferably enumerate 35～40 ℃, more preferably enumerate 36～38 ℃ so long as be fit to the condition of the fertility of equol generation bacterium as fermentation temperature.

Fermentation time can suitably be set according to the growing amount of equol, the residual quantity of daidzein class, the kind that equol produces bacterium etc., can be 1～10 usually, is preferably 2～7, more preferably 3～5 days.

Carry out under these conditions generating and accumulating in the soybean plumular axis fermentate of fermentation process gained that equol is arranged, can present the useful physiological action of equol.Equol content in the soybean plumular axis fermentate produces bacterium or fermentation condition etc. and difference according to employed equol, but the soybean plumular axis fermentate of common every dry weight (when the dry weight of soybean plumular axis fermentate is 1g), contain 1～20m g, preferably contain 2～12mg, more preferably contain 5～8mg equol.

Except that equol, can also contain daidzein classes such as daidzin, malonyl daidzin, acetyl daidzin, daidzein, DIHYDRADAIDZEIN (below, these component lists are shown " daidzein class ") in the soybean plumular axis fermentate; Genistein classes such as genistin, malonyl genistin, acetyl colors wood glycosides, genistein, dihydro genistein (below, these component lists are shown " genistein class "); Glycitein classes such as soya bean xanthosine, malonyl soya bean xanthosine, acetyl soya bean xanthosine, Glycitein, dihydro Glycitein (below; these component lists are shown " Glycitein class ") etc. various isoflavones, also can present the useful physiologically active of above-mentioned isoflavones.The content of the isoflavones in the soybean plumular axis fermentate (containing equol) can be enumerated in the soybean plumular axis fermentate of every 1g dry weight, and isoflavones is 5～20mg, is preferably 5～15mg, more preferably about 8～15mg.

In addition, from the composition aspect of equol isoflavones in addition, the isoflavones in the soybean plumular axis fermentate has the isoflavones different composition contained with soybean plumular axis.Particularly in the soybean plumular axis fermentate, the summation of the genistein class that might play a role as endocrine disturbing matters is with respect to proportional preferably being reduced to below the 15 weight % that contain of the isoflavones total amount of soybean plumular axis fermentate, more preferably be reduced to below the 12 weight %, viewpoint from the composition of isoflavones, compare with the soybean plumular axis before the fermentation, the soybean plumular axis fermentate is favourable.

As the soybean plumular axis fermentate, can enumerate the fermentate (following unit " mg " represents the total amount of each isoflavones in every 1g (dry weight) soybean plumular axis fermentate) that following isoflavones is formed particularly:

Equol: 1～20mg, preferred 2～12mg,

Daidzein class: 0.1～30mg, preferred 0.1～1.5mg,

Genistein class: 0.05～2.5mg, preferred 0.05～2mg,

Glycitein class: 0.1～4mg, preferred 2～3.5mg.

In addition, the composition ratio of each contained isoflavones can be enumerated scope shown below (following unit " weight % " expression is with respect to the ratio of the total amount of whole isoflavones contained in the soybean plumular axis fermentate) in the soybean plumular axis fermentate:

Equol 30～75 weight %, preferred 40～70 weight %, more preferably 45～70 weight %,

The daidzein class: 1～20 weight %, preferred 2～15 weight %, more preferably 4～12 weight %,

The genistein class: 0.1～20 weight %, preferred 1～15 weight %, more preferably 1～10 weight %,

Glycitein class: 10～50 weight %, preferred 15～35 weight %, more preferably 25～35 weight %.

Because soybean plumular axis fermentate of the present invention contains the isoflavones that utilizes the irrealizable composition of prior art, so, also can be described as isoflavones and contain material with above-mentioned isoflavones composition from other viewpoints.

In order to prepare the soybean plumular axis fermentate of isoflavones, especially preferably use galactococcus 20-92 (FERM BP-10036 number) to produce bacterium as equol with above-mentioned composition.

And then, owing to also contain saponin in the soybean plumular axis fermentate, so also can obtain useful physiologically active (for example antiviral activity etc.) based on the saponin effect from soybean plumular axis.Saponin content in the soybean plumular axis fermentate is as follows: the soybean plumular axis fermentate of every 1g dry weight, contain 10～80mg saponin, and be preferably 20～50mg, more preferably 30～40mg.

As mentioned above, use the ornithine equol to produce bacterium and arginine is added in the soybean plumular axis fermentate that makes its fermentation gained in the soybean plumular axis contains ornithine.As the content of ornithine contained in this soybean plumular axis fermentate, the soybean plumular axis fermentate that can enumerate every 1g dry weight particularly contains 5～2mg, preferred 8～15mg, the more preferably ornithine about 9～12mg.

The soybean plumular axis fermentate that obtains of fermentation process can be with the state after the fermentation directly as the raw material of food, pharmaceuticals, cosmetics etc. under these conditions, in addition, also can carry out drying as required and handle the dry solid shape of formation, use as above-mentioned raw materials.In order to improve the storage stability of soybean plumular axis fermentate, preferably handle by heat drying in advance and form solid-state shape.In addition, the soybean plumular axis fermentate after heat drying is handled can be implemented powdered as required and handle, and makes Powdered.

As mentioned above, soybean plumular axis fermentate of the present invention contains various useful physiological activators such as equol, so can present various physiologically actives or pharmacologically active.For example, soybean plumular axis fermentate of the present invention to prevention improve disease such as climacteric obstacle, osteoporosis, hypertrophy of the prostate, metabolic syndrome or symptom, reduction blood in the cholesterine value, to whiten, improve acne, whole intestines, improve obesity, diuresis etc. be useful.Wherein, soybean plumular axis fermentate of the present invention is to prevention or improve the indefinite private prosecution symptom of the symptom of middle and aged women or follow the symptom that menopause occurs (for example osteoporosis, climacteric obstacle etc.) particularly useful.In addition, use the ornithine equol to produce bacterium and make also to generate in the soybean plumular axis fermentate that contains arginic fermentation raw material fermentation gained and accumulates ornithine, so this soybean plumular axis fermentate also can improve liver function, promote growth hormone secretion, immunity effect, increase muscle mass, improve the useful physiological action based on ornithine such as basic metabolism ability.

When using soybean plumular axis fermentate of the present invention as raw-food material, this soybean plumular axis fermentate for example can be modulated into forms such as beverage, particle, particulate, capsule, tablet, powder, dairy products, chewing gum, arabinose (gum drop), pudding, bar (bar), other solid-state foods.The food that contains above-mentioned soybean plumular axis fermentate also has the physiologically active of other isoflavones or saponin etc. except that the useful physiologically active of equol, thus the serviceability height, health care effect excellence.In addition, use when utilizing ornithine equol generation bacterium to make the soybean plumular axis fermentate that contains arginic fermentation raw material fermentation gained, also can contain ornithine in the food, so can further improve serviceability as food.

The food that contains soybean plumular axis fermentate of the present invention is except that can be as the general food, also can be as specific food for health care, dietary supplements, functional food, patient with food etc.The food that particularly contains soybean plumular axis fermentate of the present invention is useful as dietary supplements.

Contain in the food of soybean plumular axis fermentate of the present invention, the cooperation ratio of this soybean plumular axis fermentate in this food can suitably be set according to age of the content of the kind of this food, equol, picked-up object or sex, the effect expected etc.Can enumerate a following example, with respect to the above-mentioned food of 100g, the total amount of soybean plumular axis fermentate (being converted into dry weight) is 0.1～90g, preferred 0.1～10g, more preferably 0.5～2g.

A daily intake of food that contains the soybean plumular axis fermentate is according to the equol content in the soybean plumular axis fermentate, the age of taking the photograph taker or body weight, picked-up number of times etc. and different, for example, the intake of being grown up 1 can be enumerated the amount that is equivalent to 0.1～10g soybean plumular axis fermentate.

In addition, when using soybean plumular axis fermentate of the present invention as the pharmaceuticals raw material, this soybean plumular axis fermentate can be modulated into the pharmaceutical preparation of forms such as tablet, pill, powder, liquid preparation, suspension, emulsion, granule, capsule, suppository.The pharmaceutical preparation that contains soybean plumular axis fermentate of the present invention is useful as the depressant of cholesterine value in the prevention of diseases such as climacteric obstacle (private prosecution symptom, osteoporosis, hyperlipemia that involutional symptom is indefinite), osteoporosis, hypertrophy of the prostate, metabolic syndrome or symptom or improver, the blood, medicines for relieving intestinal disorders, fat improver, diuretics etc.The pharmaceutical preparation that particularly contains the soybean plumular axis fermentate is preferred for preventing or treats the indefinite private prosecution symptom of the symptom of middle and aged women or follows the symptom that menopause occurs (for example osteoporosis, climacteric obstacle etc.).

Contain the soybean plumular axis fermentate pharmaceutical preparation give with amount according to age of the equol content in the soybean plumular axis fermentate, administration object or body weight, symptom, administration number of times etc. and different, can not stipulate without exception, for example, as 1 day dosage of adult, can enumerate the soybean plumular axis fermentate and be converted into the amount that dry weight is equivalent to 0.5～6g.

And then, when using soybean plumular axis fermentate of the present invention as the raw material of cosmetics, this soybean plumular axis fermentate can be modulated into the cosmetics of pasty state, mousse shape, gel, aqueous, emulsion form, suspension shape, paste, ointment shape, various desirable forms such as laminar.Above-mentioned cosmetics are as basic cosmetics such as emulsion, cream, washing lotion, oil and films; Washing agent such as clean agent, washing agent, health washing agent; The clean wiping agent; Various cosmetics such as cleaning agent are useful.The cosmetics that contain soybean plumular axis fermentate of the present invention can be used as to whiten and apply some make up or acne are improved and to be applied some make up.

In the cosmetics that contain soybean plumular axis fermentate of the present invention, the cooperation ratio of this soybean plumular axis fermentate in these cosmetics can suitably be set according to the kind of these cosmetics, the content of equol etc.Can enumerate a following example, with respect to the above-mentioned cosmetics of 100g, the total amount of soybean plumular axis fermentate (being converted into dry weight) is 0.1～10g, is preferably 0.5～5.

Embodiment

Below, describe the present invention in detail based on test example, embodiment etc., but the present invention is not limited to these embodiment.

Embodiment 1-3

According to composition mixed powder powder soybean plumular axis, arginine and the water shown in the table 1, modulation soybean plumular axis solution (raw material).Inoculation galactococcus 20-92 strain (FERM BP-10036 number) in this soybean plumular axis solution of 5ml is left standstill under anaerobic condition, 37 ℃ and was cultivated 96 hours.After the cultivation, 100 ℃ of following pasteurizations 1 minute, then, the dry processing under 80 ℃ condition further carried out powdered with homogenizer and handled, and obtains pulverous soybean plumular axis fermentate with the zymotic fluid (nutrient solution) of gained.

Fig. 1 represents to cultivate the equol concentration in the nutrient solution after 96 hours.Simultaneously, table 1 expression cultivate the viable count in the nutrient solution and pH after 96 hours, pulverous soybean plumular axis fermentate the amount of obtaining, and pulverous soybean plumular axis fermentate in equol concentration.Can confirm by this result, make Powdered soybean plumular axis fermentation, can generate equol with high efficiency by using equol to produce bacterium.

[table 1]

Each embodiment of # uses 3 batches Powdered soybean plumular axis to implement (N=3).Analysis result shown in the table is average ± SD value.

Embodiment 4

Contain in Powdered soybean plumular axis of 10 weight % and the arginic soybean plumular axis solution of 0.1 weight %L-inoculation galactococcus 20-92 strain (FERM BP-10036 number) at 5ml, under anaerobic condition, 37 ℃, leave standstill and cultivated 96 hours, carry out fermentation process thus.After the cultivation,, then, under 80 ℃ condition, carry out drying and handle, further carry out powdered and handle, obtain pulverous soybean plumular axis fermentate thus with homogenizer with the zymotic fluid (nutrient solution) of gained pasteurization 1 minute under 100 ℃ condition.

The Powdered soybean plumular axis fermentate of Powdered soybean plumular axis that analysis is used as raw material (before being expressed as fermentation in the table 2 and 3) and gained (being expressed as the fermentation back in the table 2 and 3) contain composition.The analysis result of soybean isoflavone is shown in table 2, and the analysis result of nutritional labeling is shown in table 3.Also can confirm by this result,, can prepare the soybean plumular axis fermentate of the equol that contains high-load by make the soybean plumular axis fermentation with galactococcus 20-92 strain.In addition can be clear and definite, the content of oligosaccharides such as melitriose or stachyose is same degree before and after fermentation, the influence of almost being fermented.On the other hand, confirm that arginine is converted into ornithine by fermentation process.Therefore, in soybean plumular axis, add arginine, carry out fermentation process, can not only generate equol thus, can also generate ornithine with galactococcus 20-92 strain.

[table 2]

The every 100g of soybean isoflavone

N.D. expression does not detect

[table 3]

The every 100g of nutritional labeling

N.D. expression does not detect

Embodiment 5-11

Except that using 7 batch the Powdered soybean plumular axis different, under the condition identical, prepare Powdered soybean plumular axis fermentate (embodiment 5-11) with the foregoing description 3 with the foregoing description 3.Analyze the composition of isoflavones contained in the soybean plumular axis fermentate of gained.The results are shown in table 4.Can confirm the equol content height of the soybean plumular axis fermentate of embodiment 5-11 by this result, contain the isoflavones of the irrealizable composition of prior art.

[table 4]

In the table, each isoflavone levels (mg) in the every 1g soybean plumular axis of the numeric representation on the top fermentate, the ratio (weight %) of the isoflavones total amount (100 weight %) that each isoflavones of the numeric representation of bottom is contained with respect to the soybean plumular axis fermentate.

Embodiment 12

Except that the Powdered soybean plumular axis that uses with the foregoing description 3 different batches, the pulverous soybean plumular axis fermentate of preparation under the condition identical with the foregoing description 3.The soybean plumular axis fermentate of every 1g gained contains 6.5mg equol, 0.6mg daidzein class, 0.6mg genistein class, 3.2mg Glycitein class.In addition, in this soybean plumular axis fermentate, glycosides former (aglycon) accounts for more than the 90 weight % of isoflavone content total amount.

Use the soybean plumular axis fermentate that obtains thus, make the tablet (every weight is 2.51g, and 1 contains the 10.9mg equol) of following composition.

Soybean plumular axis fermentate 66.7 weight %

Erythrite 33.2 weight %

Add up to 100.0 weight %

Embodiment 13

Use the soybean plumular axis fermentate that uses in the foregoing description 12, prepare the particle of following composition.

Soybean plumular axis fermentate 66.7 weight %

Erythrite 33.2 weight %

Add up to 100.0 weight %

Embodiment 14

Use the soybean plumular axis fermentate of the foregoing description 1, the following cosmetics of forming of modulation.

Soybean plumular axis fermentate 10g

Paraffin oil 60ml

Olive oil 40ml

Glycerin monostearate 50ml

Lanolin 10ml

Propane diols 30ml

Water is an amount of

Add up to 1000g

Embodiment 15

Use the soybean plumular axis fermentate of the foregoing description 1, the following cosmetics of forming of modulation.

Soybean plumular axis fermentate 10g

Paraffin oil 30ml

Olive oil 30ml

Glycerin monostearate 60ml

Lanolin 20ml

Propane diols 40ml

Water is an amount of

Add up to 1000g

Test example 1

Contain anaphylactogens such as Gym4, Gm30K, Gm28K, 7S globulin mix (β-glycinin (conglycine)), oleosin, trypsin inhibitor in the known soybean plumular axis.So, judge in the soybean plumular axis fermentate for preparing in the foregoing description whether have anaphylactogen with following test.

At first, the soybean plumular axis fermentate that obtains among an amount of embodiment 1 is added into extraction buffer solution (Tris HCl pH7.5 contains 1M EDTA, an amount of protein decomposes enzyme inhibitor), fully stirs the extraction water soluble components.Then, by removing by filter solid state component, obtain extract.Protein analysis system with Bole (bio-rad) corporate system detects contained gross protein in the extract that obtains thus.Further use protein immunoblot (Western blotting) method to detect contained main anaphylactogen (Gym4, Gm30K, Gm28K, 7S globulin mix, oleosin, trypsin inhibitor) in the extract of gained.In addition, as a comparison, use the soybean cotyledon powder, reach soybean plumular axis powder replacement soybean plumular axis fermentate, detect gross protein and anaphylactogen with identical method.

The results are shown in Fig. 2～4.Fig. 2 represents the testing result of gross protein; Fig. 3 represents Gym4, Gm30K, reaches the testing result of Gm28K; Fig. 4 represents 7S globulin mix, oleosin, reaches the testing result of trypsin inhibitor.

Can confirm by this result, reduce main anaphylactogen contained in soybean or the soybean plumular axis in the soybean plumular axis fermentate.

Claims (19)

1, a kind of soybean plumular axis fermentate that contains equol, with have assimilation be selected from daidzein glucosides, daidzein, and DIHYDRADAIDZEIN at least a daidzein class microorganism that produces the ability of equol the soybean plumular axis fermentation is obtained.

2, soybean plumular axis fermentate as claimed in claim 1, wherein, described microorganism is the lactic acid bacteria that belongs to lactococcus.

3, soybean plumular axis fermentate as claimed in claim 1, wherein, described microorganism be the Ge Shi galactococcus ( Lactococcus garvieae).

4, soybean plumular axis fermentate as claimed in claim 1 wherein, with respect to the soybean plumular axis fermentate of every dry weight, contains 0.1～20 weight % equol.

5, soybean plumular axis fermentate as claimed in claim 1, wherein, described soybean plumular axis fermentate also contains soya bean glycoside, dyewood glycoside, genistein class, soya bean xanthosine class and Glycitein class.

6, soybean plumular axis fermentate as claimed in claim 1 wherein, also contains ornithine.

7, a kind of food contains the described soybean plumular axis fermentate of claim 1.

8, food as claimed in claim 7 is dietary supplements.

9, food as claimed in claim 7, wherein, every 100g food contains the described soybean plumular axis fermentate of 0.1～90g.

10, a kind of pharmaceutical preparation contains the described soybean plumular axis fermentate of claim 1.

11, pharmaceutical preparation as claimed in claim 10 is climacteric obstacle, osteoporosis, a hypertrophy of the prostate or the prevention or the therapeutic agent of metabolic syndrome.

12, pharmaceutical preparation as claimed in claim 10 is the depressant of cholesterine value in the blood.

13, the described soybean plumular axis fermentate of claim 1 is in preparation climacteric obstacle, osteoporosis, hypertrophy of the prostate or the prevention of metabolic syndrome or the purposes in the therapeutic agent.

14, the purposes in the depressant of the described soybean plumular axis fermentate of claim 1 cholesterine value in preparation blood.

15, a kind of climacteric obstacle methods of treatment, it is characterized in that the patient of obstacle gives the described soybean plumular axis fermentate of claim 1 of effective dose to climacteric.

16, the reduction method of cholesterine value in a kind of blood is characterized in that, the patient of cholesterine value in the needs reduction blood is given the described soybean plumular axis fermentate of claim 1 of effective dose.

17, a kind of cosmetics contain the described soybean plumular axis fermentate of claim 1.

18, cosmetics as claimed in claim 17, wherein, every 100g cosmetics contain the described soybean plumular axis fermentate of 0.1～10g.

19, a kind of preparation method who contains the soybean plumular axis fermentate of equol, it is characterized in that, assimilate the microbial fermentation processing soybean plumular axis that at least a daidzein class that is selected from daidzein glucosides, daidzein and the DIHYDRADAIDZEIN produces the ability of equol with having.

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