CN101324561A - D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration - Google Patents
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration Download PDFInfo
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- CN101324561A CN101324561A CNA200710023230XA CN200710023230A CN101324561A CN 101324561 A CN101324561 A CN 101324561A CN A200710023230X A CNA200710023230X A CN A200710023230XA CN 200710023230 A CN200710023230 A CN 200710023230A CN 101324561 A CN101324561 A CN 101324561A
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Abstract
The invention relates to a kit for diagnosing/mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical/food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by utilizing a visible light analyzer and further reflecting the concentration of the D-lactic acid directly.
Description
Technical field
The present invention relates to the specific D-lactic acid of a kind of D-of having lactic acid diagnosis/determination kit, the invention still further relates to the method for measuring the D-lactic acid concn simultaneously, belong to medical science/Food Inspection determination techniques field.
Background technology
L-lactic acid is that people extremely pay close attention to and develop organic acid faster in recent years.L-lactic acid has in multi-field application prospects such as food, feed, medicine, plastics, feed, agricultural chemicals, daily-use chemical industry, papermaking and electronics industries simultaneously.
L-lactic acid is the metabolic end-products of most of biosomes.Only minority microorganism (routine genus lactubacillus, Leuconostoc) can form D-lactic acid.Exactissima diligentia detects lactate when producing sour milk, whether produces L-lactic acid with the assessment microorganism, and D-lactic acid or the two have concurrently.If there is D-lactic acid to exist, then may be that microorganism has been caused pollution.
The unusual rising of lactate concentration and multiple disease such as diseases such as diabetes, acidosis have confidential relation.L (+)-Lactate is the main intermediate product of lactate metabolism in the human body, and is present in the blood.D (-)-Lactate also exists, but content is very low, has only the 1-5% of L (+)-Lactate.
The test of blood lactic acid is as the means of a monitoring, monitoring training athlete ability, the significant data of blood lactic acid value as aspects such as measurement sportsman aerobic endurance level, anaerobic exercise ability, physical fatigue recoveries, reference during for the arrangement training.
Enzymatic analysis is the analytical approach of generally acknowledging in the world, organized by a plurality of internal authorities or mechanism adopts: as IFU (international fruit juice association of producers), AIJN (European Union's Juice and beverage production person alliance), and MEBAK, OICC, VDLUFA etc. organize widely and praise highly, and are defined as standard detecting method.
Summary of the invention
The technical problem to be solved in the present invention is: propose a kind of utilization and have the specific enzyme cycle amplification method of D-lactic acid (Enzymatic Recycling Method), enzymic colorimetric (Enzymatic ColorimetricMethod) and enzyme-linked method (Couple Reaction) technology, metering generates the rising in 400-700nm wavelength place absorbance of indoleamine chromogen (Indamine dye) or quinone-imine chromogen (Quioneimine dye) cause by integrated enzyme reaction, measured the method for D-lactic acid concn, simultaneously, the present invention also will provide in order to realize the D-lactic acid diagnosis/determination kit of this method, adopt this reagent not only can be visible light analysis instrument or half, carrying out the D-lactic acid concn on the automatic clinical chemistry analyzer measures, and finding speed is fast, sensitivity is big, the accuracy height, thereby can obtain practical applying.
D-lactic acid concn assay method principle of the present invention is as follows:
D-lactic acid+2 ferricytochrome c
The D-lactic dehydrogenasePyruvic acid+2 ferricytochrome c
Pyruvic acid+ammonium ion+reduced coenzyme
Alanine dehydrogenaseL-alanine+water+coenzyme
Alanine+water+oxygen
Glycine oxidaseAmmonium ion+pyruvic acid+hydrogen peroxide
Hydrogen peroxide+reduced form chromogen combination
PeroxidaseIndoleamine chromogen or quinone-imine chromogen+water
This method is used has the specific D-lactic dehydrogenase of D-lactic acid (D-Lactatedehydrogenase; EC 1.1.2.4; EC 1.1.2.5) enzyme connection alanine dehydrogenase (alaninedehydrogenase; EC 1.4.1.1), glycine oxidase (glycine oxidase; EC 1.4.3.19) and peroxidase (peroxidase; EC 1.11.1.7) enzymatic reaction terminal point/speed ratio color method.The D-lactic dehydrogenase is as the effect enzyme, and function is that D-lactic acid enzymolysis is produced pyruvic acid.Alanine dehydrogenase is also as the effect enzyme, and its enzymatic catalysis makes pyruvic acid produce alanine; Glycine oxidase can become alanine again pyruvic acid again and again once more as cyclophorase, and it is recycling that pyruvic acid constantly is repeated; Constantly repetitive cycling produces hydrogen peroxide simultaneously.Peroxidase (peroxidase; EC 1.11.1.7) as the colour developing enzyme, by with the effect of hydrogen peroxide, make colourless reduced form chromogen combination be oxidized to coloured dyestuff, thereby can pass through the visible light analysis instrument, measure the light absorption size degree/speed of dyestuff-indoleamine chromogen (Indamine dye) or quinone-imine chromogen (Quioneimine dye)-content height at 400-700nm (difference according to the combination of reduced form chromogen is decided) wavelength place, draw amino acid (nitrogen) concentration measurement result.
Experiment shows, takes all factors into consideration from the accuracy of measurement result and economy two aspects of preparation cost, and the D-lactic acid diagnosis/determination kit of the present invention of following composition relation is comparatively desirable:
Damping fluid 100mmol/L
Stabilizing agent 500mmol/L
Reduced coenzyme 0.25mmol/L
2 ferricytochrome c 10mmol/L
Ammonium ion 20mmol/L
D-lactic dehydrogenase 10 000U/L
Alanine dehydrogenase 10000U/L
Glycine oxidase 10000U/L
Peroxidase 30000U/L
Reduced form chromogen combination 0.1---20mmol/L
Described reduced form chromogen combination (Chromogen) can be any one combinations of pairs in following 28 combinations:
3-methyl-2-2-thiobenzimide hydrazone
Carbolic acid
3-methyl-2-2-thiobenzimide hydrazone
N-ethyl-N-(3-thiopropyl)-m-thialdine amine
3-methyl-2-2-thiobenzimide hydrazone
N, the two ethyls of N--m-toluidine
3-methyl-2-2-thiobenzimide hydrazone
2, the two chlorine carbolic acids of 4-
3-methyl-2-2-thiobenzimide hydrazone
2,4,6-three bromo-3-hydroxyl-benzene sulfonic acid
3-methyl-2-2-thiobenzimide hydrazone
3, the two chlorine carbolic acid sulfonic acid of 5-
3-methyl-2-2-thiobenzimide hydrazone
3, the two chloro-2-hydroxyl-benzene sulfonic acids of 5-
3-methyl-2-2-thiobenzimide hydrazone
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine sodium salt
3-methyl-2-2-thiobenzimide hydrazone
Sa Xiu Hydroxyalkyl yl benzoic acid
3-methyl-2-2-thiobenzimide hydrazone
Two methylanilines
3-methyl-2-2-thiobenzimide hydrazone
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine
3-methyl-2-2-thiobenzimide hydrazone
2,2 '-AZINO-two (3-ethylbenzene thiazole-6-sulfonic acid)
3-methyl-2-2-thiobenzimide hydrazone
4-Hydroxyalkyl base-3-methoxy benzoic acid
3-methyl-2-2-thiobenzimide hydrazone
3-methyl-ethyl-Hydroxyalkyl base aniline
The amino anti-arsenic of 4-
Carbolic acid
The amino anti-arsenic of 4-
N-ethyl-N-(3-thiopropyl)-m-thialdine amine
The amino anti-arsenic of 4-
N, the two ethyls of N--m-toluidine
The amino anti-arsenic of 4-
2, the two chlorine carbolic acids of 4-
The amino anti-arsenic of 4-
2,4,6-three bromo-3-hydroxyl-benzene sulfonic acid
The amino anti-arsenic of 4-
3, the two chlorine carbolic acid sulfonic acid of 5-
The amino anti-arsenic of 4-
3, the two chloro-2-hydroxyl-benzene sulfonic acids of 5-
The amino anti-arsenic of 4-
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine sodium salt
The amino anti-arsenic of 4-
Sa Xiu Hydroxyalkyl yl benzoic acid
The amino anti-arsenic of 4-
Two methylanilines
The amino anti-arsenic of 4-
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine
The amino anti-arsenic of 4-
2,2 '-AZINO-two (3-ethylbenzene thiazole-6-sulfonic acid)
The amino anti-arsenic of 4-
4-Hydroxyalkyl base-3-methoxy benzoic acid
The amino anti-arsenic of 4-
3-methyl-ethyl-Hydroxyalkyl base aniline
。
D-lactic acid diagnosis/determination kit of the present invention can be single agent, comprising: damping fluid, stabilizing agent, reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, the combination of reduced form chromogen.Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
Also above-mentioned single agent reagent can be made into following pair of agent reagent:
Reagent 1
Damping fluid, stabilizing agent, the combination of reduced form chromogen, reduced coenzyme, 2 ferricytochrome c, ammonium ion.
Reagent 2
Damping fluid, stabilizing agent, peroxidase, the combination of reduced form chromogen, D-lactic dehydrogenase, alanine dehydrogenase, glycine oxidase.
Peroxidase, the combination of reduced form chromogen, reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, the position of glycine oxidase in reagent 1 or reagent 2 can not limit.Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
Also above-mentioned single agent reagent can be made into following three doses of reagent:
Reagent 1
Damping fluid, stabilizing agent, the combination of reduced form chromogen, reduced coenzyme, 2 ferricytochrome c, ammonium ion.
Reagent 2
Damping fluid, stabilizing agent, the combination of reduced form chromogen, alanine dehydrogenase, glycine oxidase, peroxidase.
Reagent 3
Damping fluid, stabilizing agent, D-lactic dehydrogenase.
Peroxidase, the combination of reduced form chromogen, reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, the position of glycine oxidase in reagent 1, reagent 2 or reagent 3 can not limit.Kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
Embodiment
The present invention is further illustrated below in conjunction with examples of implementation.
Embodiment one
The D-lactic acid diagnosing/determining reagent of present embodiment is single reagent, comprising:
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Reduced coenzyme 0.25mmol/L
2 ferricytochrome c 10mmol/L
Ammonium ion 20mmol/L
D-lactic dehydrogenase 10 000U/L
Alanine dehydrogenase 10000U/L
Glycine oxidase 10000U/L
Peroxidase 30000U/L
The amino anti-arsenic 2mmol/L of 4-
Carbolic acid 10mmol/L
Reagent divides the bottle of packing into after all dissolving and preparing, and carries out freeze drying, makes powdered reagent; Before the use, add pure water, use after redissolving.
On automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, test predominant wavelength 505nm, test commplementary wave length 600nm, the volume ratio of tested D-lactic acid sample and reagent is 1/25, and the Direction of Reaction is positive reaction (reaction of rising), and detection method is terminal point/rate method, about about 1 minute of time delay is about 3 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 505nm absorbance rises, thereby calculates D-concentration of lactic acid size.
Embodiment two
The D-lactic acid diagnosing/determining reagent of present embodiment is double reagent, comprising:
Reagent 1
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 50mmol/L
Reduced coenzyme 0.25mmol/L
2 ferricytochrome c 10mmol/L
Ammonium ion 20mmol/L
2,4,6-three bromo-3-hydroxyl-benzene sulfonic acid 0.2mmol/L
Reagent 2
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
D-lactic dehydrogenase 10 000U/L
Alanine dehydrogenase 10000U/L
Glycine oxidase 10000U/L
Peroxidase 25000U/L
The amino anti-arsenic 0.6mmol/L of 4-
Reagent divides the bottle of packing into after all dissolving and preparing, and makes liquid double reagent, can directly use.
On automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, test predominant wavelength 546nm, test commplementary wave length 600nm, the volume ratio of tested D-lactic acid sample and reagent is 1/20, and the Direction of Reaction is positive reaction (reaction of rising), and detection method is terminal point/rate method, about about 1 minute of time delay is about 3 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 546nm absorbance rises, thereby calculates D-concentration of lactic acid size.
Embodiment three
The D-lactic acid diagnosing/determining reagent of present embodiment is three reagent, comprising:
Reagent 1
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 50mmol/L
Reduced coenzyme 0.25mmol/L
2 ferricytochrome c 10mmol/L
Ammonium ion 20mmol/L
3-methyl-2-2-thiobenzimide hydrazone 0.6mmol/L
Reagent 2
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
Alanine dehydrogenase 10000U/L
Glycine oxidase 10000U/L
Peroxidase 20000U/L
Two methylaniline 2mmol/L
Reagent 3
Three (ethyloic) aminomethane-hydrochloride buffer 100mmol/L
Stabilizing agent 500mmol/L
D-lactic dehydrogenase 10 000U/L
Reagent divides the bottle of packing into after all dissolving and preparing, and makes liquid three reagent, can directly use.
On automatic clinical chemistry analyzer, set: 37 ℃ of temperature, 10 minutes reaction time, test predominant wavelength 578nm, test commplementary wave length 660nm, the volume ratio of tested D-lactic acid sample and reagent is 1/30, and the Direction of Reaction is positive reaction (reaction of rising), and detection method is terminal point/rate method, about about 1 minute of time delay is about 3 minutes detection times.
After adding sample and reagent, make them mixed and have reaction, reactant places under the Biochemical Analyzer the most at last, detects degree/speed that predominant wavelength 578nm absorbance rises, thereby calculates D-concentration of lactic acid size.
The applicant adopts other various reduced form chromogens combinations of putting down in writing in the above summary of the invention all can reach purpose of the present invention through experimental verification, in view of situation such as determination step and above embodiment roughly the same, do not separately enumerate.
In a word, experiment showed, and adopt assay method of the present invention can draw required measurement result by the visible light analysis instrument fully, and highly sensitive, degree of accuracy good, not do not polluted by inside and outside source object quantity.
Claims (7)
1. D-lactic acid concn assay method with the specific enzyme cycle amplification method of D-lactic acid, enzymic colorimetric and enzyme-linked method technology, its method principle is as follows:
D-lactic acid+2 ferricytochrome c
The D-lactic dehydrogenasePyruvic acid+
2 ferricytochrome c
Pyruvic acid+ammonium ion+reduced coenzyme
Alanine dehydrogenaseThe L-alanine
+ water+coenzyme
Alanine+water+oxygen
Glycine oxidaseAmmonium ion+pyruvic acid+
Hydrogen peroxide
Hydrogen peroxide+reduced form chromogen combination
PeroxidaseThe indoleamine chromogen or
Quinone-imine chromogen+water
The end reaction thing is placed under the visible light analysis instrument, detect light absorption speed/degree that indoleamine chromogen or quinone-imine chromogen content height are directly reflected in the 400-700nm place, draw D-lactic acid concn size measurement result.
2. D-lactic acid diagnosis/determination kit, principal ingredient comprises:
Damping fluid 20-500mmol/L
Stabilizing agent 1-4000mmol/L
Reduced coenzyme 0.1-0.35mmol/L
2 ferricytochrome c 1-50mmol/L
Ammonium ion 1-50mmol/L
D-lactic dehydrogenase 10 00-80000U/L
Alanine dehydrogenase 1000-80000U/L
Glycine oxidase 1000-80000U/L
Peroxidase 500-80000U/L
Reduced form chromogen combination 0.1-20mmol/L
It is characterized in that: kit can be a dry powder, and use the back that is dissolved in water before use; Also can be mixed with liquid reagent, directly use.
3. according to the described D-lactic acid of claim 2 diagnosis/determination kit, it is characterized in that:
Form single agent reagent by damping fluid, stabilizing agent, reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen.
4. according to the described D-lactic acid of claim 2 diagnosis/determination kit, it is characterized in that:
Form two agent reagent by damping fluid, stabilizing agent, reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen; Reagent 1 is made up of damping fluid, stabilizing agent, reduced coenzyme, 2 ferricytochrome c, ammonium ion, reduced form chromogen; Reagent 2 is made up of damping fluid, stabilizing agent, D-lactic dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen.Peroxidase, the combination of reduced form chromogen, reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, the position of glycine oxidase in reagent 1 or reagent 2 can not limit.
5. according to the described D-lactic acid of claim 2 diagnosis/determination kit, it is characterized in that:
Form multi-agent reagent by damping fluid, stabilizing agent, reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen; Reagent 1 is made up of damping fluid, stabilizing agent, reduced coenzyme, 2 ferricytochrome c, ammonium ion, reduced form chromogen; Reagent 2 is made up of damping fluid, stabilizing agent, alanine dehydrogenase, glycine oxidase, peroxidase, reduced form chromogen; Reagent 3 is made up of damping fluid, stabilizing agent, D-lactic dehydrogenase.Peroxidase, reduced form chromogen combination reduced coenzyme, 2 ferricytochrome c, ammonium ion, D-lactic dehydrogenase, alanine dehydrogenase, the position of glycine oxidase in reagent 1, reagent 2 or reagent 3 can not limit.
6. according to the described D-lactic acid of claim 2 diagnosis/determination kit, it is characterized in that: also comprise stabilizing agent 1-4000mmol/L or 0.1%-100% volume ratio.Described stabilizing agent is: ammonium sulfate (Ammonia Sulfate), glycerine (Glycerol), propylene glycol (Propylene Glycol), ethylene glycol (Ethylene glycol) and at least one of the preservatives.
7. according to the described D-lactic acid of claim 2 diagnosis/determination kit, it is characterized in that: described reduced form chromogen combination can be any combinations of pairs in following 28 combinations:
3-methyl-2-2-thiobenzimide hydrazone
Carbolic acid
3-methyl-2-2-thiobenzimide hydrazone
N-ethyl-N-(3-thiopropyl)-m-thialdine amine
3-methyl-2-2-thiobenzimide hydrazone
N, the two ethyls of N--m-toluidine
3-methyl-2-2-thiobenzimide hydrazone
2, the two chlorine carbolic acids of 4-
3-methyl-2-2-thiobenzimide hydrazone
2,4,6-three bromo-3-hydroxyl-benzene sulfonic acid
3-methyl-2-2-thiobenzimide hydrazone
3, the two chlorine carbolic acid sulfonic acid of 5-
3-methyl-2-2-thiobenzimide hydrazone
3, the two chloro-2-hydroxyl-benzene sulfonic acids of 5-
3-methyl-2-2-thiobenzimide hydrazone
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine sodium salt
3-methyl-2-2-thiobenzimide hydrazone
Sa Xiu Hydroxyalkyl yl benzoic acid
3-methyl-2-2-thiobenzimide hydrazone
Two methylanilines
3-methyl-2-2-thiobenzimide hydrazone
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine
3-methyl-2-2-thiobenzimide hydrazone
2,2 '-AZINO-two (3-ethylbenzene thiazole-6-sulfonic acid)
3-methyl-2-2-thiobenzimide hydrazone
4-Hydroxyalkyl base-3-methoxy benzoic acid
3-methyl-2-2-thiobenzimide hydrazone
3-methyl-ethyl-Hydroxyalkyl base aniline
The amino anti-arsenic of 4-
Carbolic acid
The amino anti-arsenic of 4-
N-ethyl-N-(3-thiopropyl)-m-thialdine amine
The amino anti-arsenic of 4-
N, the two ethyls of N--m-toluidine
The amino anti-arsenic of 4-
2, the two chlorine carbolic acids of 4-
The amino anti-arsenic of 4-
2,4,6-three bromo-3-hydroxyl-benzene sulfonic acid
The amino anti-arsenic of 4-
3, the two chlorine carbolic acid sulfonic acid of 5-
The amino anti-arsenic of 4-
3, the two chloro-2-hydroxyl-benzene sulfonic acids of 5-
The amino anti-arsenic of 4-
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine sodium salt
The amino anti-arsenic of 4-
Sa Xiu Hydroxyalkyl yl benzoic acid
The amino anti-arsenic of 4-
Two methylanilines
The amino anti-arsenic of 4-
N-ethyl-N-(2-Hydroxyalkyl base-3-thiopropyl)-m-toluidine
The amino anti-arsenic of 4-
2,2 '-AZINO-two (3-ethylbenzene thiazole-6-sulfonic acid)
The amino anti-arsenic of 4-
4-Hydroxyalkyl base-3-methoxy benzoic acid
The amino anti-arsenic of 4-
3-methyl-ethyl-Hydroxyalkyl base aniline
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710023230XA CN101324561A (en) | 2007-06-13 | 2007-06-13 | D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710023230XA CN101324561A (en) | 2007-06-13 | 2007-06-13 | D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration |
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| Publication Number | Publication Date |
|---|---|
| CN101324561A true CN101324561A (en) | 2008-12-17 |
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ID=40188185
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109459428A (en) * | 2018-10-17 | 2019-03-12 | 迪瑞医疗科技股份有限公司 | A kind of lactate detection drying chemical reagent paper and preparation method thereof |
| CN110305823A (en) * | 2018-11-16 | 2019-10-08 | 江南大学 | Using the method and bacterial strain of production of lactic acid l-Alanine |
-
2007
- 2007-06-13 CN CNA200710023230XA patent/CN101324561A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109459428A (en) * | 2018-10-17 | 2019-03-12 | 迪瑞医疗科技股份有限公司 | A kind of lactate detection drying chemical reagent paper and preparation method thereof |
| CN110305823A (en) * | 2018-11-16 | 2019-10-08 | 江南大学 | Using the method and bacterial strain of production of lactic acid l-Alanine |
| CN110305823B (en) * | 2018-11-16 | 2021-05-04 | 江南大学 | Method and strain for producing L-alanine by adopting lactic acid |
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Open date: 20081217 |