CN101322662B - Dental implant surface extracellular matrix biomimetic modification method - Google Patents
Dental implant surface extracellular matrix biomimetic modification method Download PDFInfo
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- CN101322662B CN101322662B CN200710069243A CN200710069243A CN101322662B CN 101322662 B CN101322662 B CN 101322662B CN 200710069243 A CN200710069243 A CN 200710069243A CN 200710069243 A CN200710069243 A CN 200710069243A CN 101322662 B CN101322662 B CN 101322662B
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Abstract
The invention discloses a preparation method for carrying out extracellular matrix biomimetic treatment on the surfaces of any parts of a metal tooth implanting system which are in contact with hard and soft tissues in the body. The surface of an implant is charged and the implant is alternately dipped in extracellular matrix solutions with opposite electrical properties, stands for a period of time, and is taken out for washing; the procedure is repeated for a plurality of times and finally cross bonding is conducted to obtain an extracellular matrix self-assembly film. The method has simple preparation procedure and does not need complex instruments, the film-forming ability is not affected by the sizes and shapes of different parts of the implant, the safety is high, the obtained tooth implanting system can facilitate the healing and stabilization of the hard and soft tissues around the implant simultaneously, thus reducing complication of implant restoration and increasing long-term success rate.
Description
Technical field
The present invention relates to a kind of method of carrying out biomimetic modification at the metal dental implant surface.Especially, relate to a kind of method of utilizing layer upon layer electrostatic self assembly principle to prepare the extracellular matrix coating at each parts surface of pure-titanium tooth implantation.
Background technology
Different with other tissue and the organ of body, oral implant rehabilitation has particularity and complexity at environment of living in and function aspects thereof, in the implanted jawbone sclerous tissues of all implantation bodys, must pass the soft tissue inlet port intracavity that covers the osseous tissue surface, be connected with artificial tooth, the clinical practice development of repairing in recent years along with implant denture, people recognize that gradually the attachment loss of mouth cavity planting body surrounding soft tissue is to cause one of major reason that implant denture proves an abortion, and good implantation body-soft tissue interface is that the formation of sealing biology is to guarantee that plantation repairs the key factor of good prognosis.Very ripe to the research of implantation body-bone interface at present, various material surface treatment methods have guaranteed that implant denture has bone support preferably, and form correlatedly be with tens kinds of implantation body's bodies (implantation body bone contact site) surface treatment method nearly, present various implant systems as
ITI, IMZ, Frialite-2, Replace etc. are single machine glazed finish to the implantation body top that contacts with soft tissue and the processing of base station.Find a kind of method under the prerequisite that does not change pattern, simultaneously each parts of implantation body are carried out surface modification, guaranteeing the synestotic sealing process that adheres to, strengthens gingiva tissue that improves the bone regeneration around implant gingiva tissue simultaneously of implantation body, be the key of present planting material surface modification.
Summary of the invention
The object of the present invention is to provide a kind of easy biomimetic modification method of human body not being had the dental implant surface of potential hazard.Relate to each parts that the tooth implant system contacts with body soft or hard tissue.
The objective of the invention is to be achieved through the following technical solutions: a kind of method of carrying out extracellular matrix biomimetic modification on the pure-titanium tooth implantation surface, it is characterized in that, may further comprise the steps:
(1) pure-titanium tooth implantation surface chargeization.
(2) preparation is electropositive and electronegative extracellular matrix solution.
(3) pure-titanium tooth implantation with electric chargeization alternately immerses in two kinds of opposite electrical extracellular matrix solution of band, take out after leaving standstill certain hour, rinse well and dry up, above process circulates, carry out UV-crosslinkedly at last, can obtain the tooth implantation system of the extracellular matrix biomimetic modification of different thickness.
The present invention has following technique effect, not only has bigger bone biocompatibility with tooth implant provided by the invention, the growth of energy rapid induction surrounding bone tissue, also have and promote the gingiva adherent double effects that heals, can promote tooth implant interface soft or hard organization healing and stable, reduce plantation and repair complication, improve success rate at a specified future date.
The specific embodiment
Extracellular matrix can send signal to cell by the specific receptor of cell surface, by cytoskeleton or various signal transduction pathway signal is conducted to Cytoplasm and even nucleus, basic vital movement such as pair cell survival, form, function, metabolism, propagation, differentiation, migration has considerable influence.
Characteristics of the present invention are with easy electrostatic attraction layer-layer self-assembling technique, employing does not have the extracellular matrix composition of any potential hazard to body, the extracellular matrix that forms self assembly at the titanium implant surface of electric chargeization is bionical, thereby find the stable bioactivity coatings of a kind of high-performance, form a kind of desirable healthy soft or hard organizational interface of implantation body, improve the success rate at a specified future date of implant denture.
Describe embodiments of the invention below in detail.
Embodiment 1
With ultrasonic cleaning 10 minutes in acetone and dehydrated alcohol successively such as pure-titanium tooth implantation and base station, deionized water washes repeatedly and dries up, and put into 80 ℃ of 8.8mol/L H2O2 and 0.1mol/L HCL mixed solutions 20 minutes, after high pure nitrogen dries up, immerse the 1mg/ml collagen solution (PH3-4 of positively charged, contain 0.1mol/L NaCL) in, leave standstill after 30 minutes and take out, deionized water washes repeatedly, and nitrogen dries up, and immerses in electronegative 0.5mg/ml sodium hyaluronate solution 30 minutes again, deionized water washes repeatedly, nitrogen dries up, and immerses collagen solution again, and circulation repeatedly, can obtain the extracellular matrix self assembly coating of different thickness respectively, outermost layer is a collagen layer, and it is UV-crosslinked to dry up the back, can obtain the system of implantation body of extracellular matrix biomimetic modification.
Embodiment 2
With ultrasonic cleaning 10 minutes in acetone and dehydrated alcohol successively such as pure-titanium tooth implantation and base station, deionized water washes repeatedly and dries up, and put into 80 ℃ of 8.8mol/L H2O2 and 0.1mol/L HCL mixed solutions 20 minutes, after high pure nitrogen dries up, immerse the 1mg/ml collagen solution (PH3-4 of positively charged, contain 0.1mol/L NaCL) in, leave standstill after 30 minutes and take out, deionized water washes repeatedly, and nitrogen dries up, and immerses in electronegative 1mg/ml chondroitin sulfate cellulose solution 30 minutes again, deionized water washes repeatedly, nitrogen dries up, and immerses collagen solution again, and circulation repeatedly, can obtain the extracellular matrix self assembly coating of different thickness respectively, outermost layer is a collagen layer, and it is UV-crosslinked to dry up the back, can obtain the system of implantation body of extracellular matrix biomimetic modification.
Embodiment 3
With ultrasonic cleaning 10 minutes in acetone and dehydrated alcohol successively such as pure-titanium tooth implantation and base station, deionized water washes repeatedly and dries up, put into the PEI polyelectrolyte solution and make surperficial positively charged, after high pure nitrogen dries up, immerse to leave standstill in electronegative 0.5mg/ml sodium hyaluronate solution after 20 minutes and take out, deionized water washes repeatedly, nitrogen dries up, immerse the 1mg/ml collagen solution (PH3-4 of positively charged again, contain 0.1mol/L NaCL) in, deionized water washes repeatedly after 20 minutes, and nitrogen dries up, and circulation repeatedly, can obtain the extracellular matrix self assembly coating of different thickness respectively, outermost layer is a collagen layer, and it is UV-crosslinked to dry up the back, can obtain the system of implantation body of extracellular matrix biomimetic modification.
The foregoing description is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.
Claims (3)
1. a method of carrying out extracellular matrix biomimetic modification on the pure-titanium tooth implantation surface is characterized in that, may further comprise the steps: (1) pure-titanium tooth implantation surface chargeization; (2) preparation is electropositive and electronegative extracellular matrix solution; (3) pure-titanium tooth implantation with electric chargeization alternately immerses in two kinds of opposite electrical extracellular matrix solution of band, take out after leaving standstill certain hour, rinse well and dry up, above process circulates, carry out UV-crosslinkedly at last, can obtain the tooth implantation system of the extracellular matrix biomimetic modification of different thickness.
2. method according to claim 1 is characterized in that: comprise the surface modification to tooth implant and base station.
3. method according to claim 1 is characterized in that: tooth implant synosteosis position can be carried out the surface roughening processing earlier, thereby improves the synosteosis area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710069243A CN101322662B (en) | 2007-06-14 | 2007-06-14 | Dental implant surface extracellular matrix biomimetic modification method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710069243A CN101322662B (en) | 2007-06-14 | 2007-06-14 | Dental implant surface extracellular matrix biomimetic modification method |
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| Publication Number | Publication Date |
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| CN101322662A CN101322662A (en) | 2008-12-17 |
| CN101322662B true CN101322662B (en) | 2010-05-26 |
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| CN200710069243A Expired - Fee Related CN101322662B (en) | 2007-06-14 | 2007-06-14 | Dental implant surface extracellular matrix biomimetic modification method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961508A (en) * | 2010-09-30 | 2011-02-02 | 浙江大学 | Method for preparing polyelectrolyte composite coating |
| CN102085388A (en) * | 2011-01-28 | 2011-06-08 | 浙江大学 | Preparation method of hybrid coating containing polypeptide chain segment |
| CN103751847A (en) * | 2013-11-25 | 2014-04-30 | 同济大学 | Preparation method of tissue-regeneration-promoting controlled-release multiple-growth-factor self-assembled coating |
| CN107117962B (en) * | 2017-05-04 | 2019-08-16 | 中山市瑞丰医疗器械有限公司 | A kind of preparation method of the dental all-ceramics repair materials based on electrostatic self-assembled technology |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107742A (en) * | 1994-12-08 | 1995-09-06 | 中国医学科学院生物医学工程研究所 | Tissue leading regeneration collagen film |
| CN1279596A (en) * | 1997-12-02 | 2001-01-10 | 清水庆彦 | Artificial tooth and process for producing the same |
| US6254634B1 (en) * | 1998-06-10 | 2001-07-03 | Surmodics, Inc. | Coating compositions |
| CN1515324A (en) * | 2003-08-25 | 2004-07-28 | 浙江大学 | Method for layer-layer self-assembling and fixing biological active factor on tissue engineering material |
| CN1817319A (en) * | 2005-12-26 | 2006-08-16 | 浙江大学 | Production of porous structure with biological activity on surface of pure-titanium tooth implantation |
-
2007
- 2007-06-14 CN CN200710069243A patent/CN101322662B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107742A (en) * | 1994-12-08 | 1995-09-06 | 中国医学科学院生物医学工程研究所 | Tissue leading regeneration collagen film |
| CN1279596A (en) * | 1997-12-02 | 2001-01-10 | 清水庆彦 | Artificial tooth and process for producing the same |
| US6254634B1 (en) * | 1998-06-10 | 2001-07-03 | Surmodics, Inc. | Coating compositions |
| CN1515324A (en) * | 2003-08-25 | 2004-07-28 | 浙江大学 | Method for layer-layer self-assembling and fixing biological active factor on tissue engineering material |
| CN1817319A (en) * | 2005-12-26 | 2006-08-16 | 浙江大学 | Production of porous structure with biological activity on surface of pure-titanium tooth implantation |
Non-Patent Citations (1)
| Title |
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| 洪咏龙.牙骨质基质提取物的提取及促牙龈成纤维细胞_成骨细胞在金属钛表面上粘附与移行的实验.2006,全文. * |
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| CN101322662A (en) | 2008-12-17 |
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