CN101308116A - Agarose gel electrophoresis dynamic imaging method - Google Patents
Agarose gel electrophoresis dynamic imaging method Download PDFInfo
- Publication number
- CN101308116A CN101308116A CNA2008100586559A CN200810058655A CN101308116A CN 101308116 A CN101308116 A CN 101308116A CN A2008100586559 A CNA2008100586559 A CN A2008100586559A CN 200810058655 A CN200810058655 A CN 200810058655A CN 101308116 A CN101308116 A CN 101308116A
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- Prior art keywords
- electrophoresis
- ultraviolet light
- gel electrophoresis
- light source
- agarose gel
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- 238000003384 imaging method Methods 0.000 title claims abstract description 25
- 238000000246 agarose gel electrophoresis Methods 0.000 title claims abstract description 9
- 238000001962 electrophoresis Methods 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 42
- 239000000499 gel Substances 0.000 claims abstract description 21
- 238000001228 spectrum Methods 0.000 claims abstract description 14
- 238000010586 diagram Methods 0.000 claims description 7
- 238000001502 gel electrophoresis Methods 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 102000039446 nucleic acids Human genes 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 8
- 239000000523 sample Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- NOIIUHRQUVNIDD-UHFFFAOYSA-N 3-[[oxo(pyridin-4-yl)methyl]hydrazo]-N-(phenylmethyl)propanamide Chemical compound C=1C=CC=CC=1CNC(=O)CCNNC(=O)C1=CC=NC=C1 NOIIUHRQUVNIDD-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides a dynamic imaging method used for agarose gel electrophoresis. The method dynamically acquires the continuously passing spectrum band information in the electrophoresis process through an ultraviolet light source arranged under an electrophoresis tank and a CCD imaging component above the electrophoresis tank, meanwhile, an embedded computer system generates a spectrum band image which is displayed on a built-in LCD display screen in real time. The method has the advantages that the electrophoresis process is simultaneous with the imaging process, process results can be observed in real time on the display screen in the electrophoresis process without taking out a gel tray. The excessive long electrophoresis time will not influence the results. The required electrophoresis voltage can also be low relatively because the gel tray can be rather short in the method.
Description
Technical field
The present invention relates to the bands of a spectrum dynamic imagery method in the gel electrophoresis imaging technique in the Biochemistry Experiment, particularly agarose gel electrophoresis analytic process.
Background technology
Electrophoresis is an experimental technique commonly used in the biological chemistry.And nucleic acid electrophoresis is to identify one of means that nucleic acid is the most frequently used (the present invention is exactly a kind of new image formation method that proposes at the nucleic acid gel electrophoresis).
At present, laboratory amplifying nucleic acid gel image device generally includes a ultraviolet light electrophoresis gel scope, gel imaging system and a robot calculator and corresponding image analysis processing software.The first step of imaging process obtains after with electrophoresis, and the gel offset plate that is loaded with nucleic acid places on the scope, when ultraviolet light passes gel, makes the nucleic acid that has fluorescent agent present the band diagram picture under the effect of ultraviolet light; Second step of imaging process is to obtain the band diagram picture by digital camera on the gel imaging system or scanner, is sent in the robot calculator by cable; The 3rd step was by the spectral band analysis process software of computing machine, bands of a spectrum observed and handled, and with result's preservation of depositing.Owing to limit by principle of work, the electrophoresis process of this classic method and imaging process are separately carried out.Thereby, can not continuous imaging in electrophoresis process, also just mean, can not carry out Real Time Observation to the bands of a spectrum in the electrophoresis process, generally all be ability imaging after electrophoresis is finished, and observes if need midway, also must close the electrophoresis apparatus power supply, pull the gel offset plate out, repeat above-mentioned steps.So traditional electrophoresis formation method is a kind of quiescent imaging method.And the classic method equipment needed thereby is many, troublesome poeration, and the also difficult correct time of determining electrophoresis, and electrophoresis time is long, the laboratory sample bands of a spectrum can pass gel, even might cause the failure of an experiment.
Summary of the invention
The objective of the invention is to overcome the deficiency of conventional electrophoretic method, a kind of method of agarose gel electrophoresis dynamic imaging is provided, this method makes electrophoresis process and imaging process to carry out simultaneously.
The present invention solves the method that its technical matters adopts: in (agarose) gel electrophoresis process, ultraviolet light is radiated on the gel, generates the band diagram picture by imageing sensor and computer real-time, and electrophoresis process and imaging process are carried out simultaneously.A ultraviolet light source promptly is set below electrophoresis tank, and the correspondence position above electrophoresis tank, adorn a line array CCD image-forming assembly.
Constituting of a whole set of imaging device that this method is related: in electrophoresis tank, be provided with gel offset plate, electrode and damping fluid, there is a shadow shield below of electrophoresis tank, below shadow shield, be provided with the ultraviolet light source of band snoot, the position corresponding with ultraviolet light source is provided with the line array CCD image-forming assembly above electrophoresis tank, this assembly is connected with an embedded computer system, and this computer system is connected to a built-in LCD display.
During work, the gel offset plate lies in a horizontal plane in the bottom and can see through in the electrophoresis tank of ultraviolet light, after starting the electrophoresis working routine, electrode adds electrophoretic voltage, electrophoresis begins to carry out, nucleic acid samples passes through between ultraviolet light source and image-forming assembly gradually, and in the sample time of each setting, ultraviolet light source is once glittering, light passes a very narrow transverse slot from bottom to top, form a horizontal light belt, when light passes nucleic acid samples in the gel, by the line array CCD image-forming assembly, obtain the image information of horizontal delegation bands of a spectrum, along with the carrying out of electrophoresis, by built-in embedded computer system, the bands of a spectrum image information that an every trade is obtained, speed according to time and sample electrophoresis, calculate the relevant position of bands of a spectrum, and by built-in LCD display in electrophoresis, dynamically demonstrate the band diagram picture.Adjust the sample period, can change the radial resolution of image.
The invention has the beneficial effects as follows that electrophoresis process and imaging process are carried out simultaneously, in the electrophoresis process, can be on display screen to process Real Time Observation as a result, and need not to pull out the gel offset plate.Even electrophoresis time is long, can not exert an influence to the result yet.Because the gel offset plate can be shorter in the method, so required electrophoretic voltage also can be relatively low.
Description of drawings
Fig. 1 is a principle schematic of the present invention.
Among the figure: 1-ultraviolet light source, 2-snoot, 3-shadow shield, 4-electrophoresis tank, 5-electrode, 6-bands of a spectrum, 7-sample, 8-sample well, 9-gel offset plate, 10-damping fluid, 11-line array CCD image-forming assembly, 12-embedded computer system, 13-LCD display screen.
Embodiment
Embodiment: as shown in Figure 1, constituting of a whole set of imaging device that this method is related: horizontal positioned has gel offset plate 9 in electrophoresis tank 4, shadow shield 3 is arranged at the bottom of electrophoresis tank, below shadow shield, be provided with the ultraviolet light source 1 of band snoot 2, the position corresponding with ultraviolet light source is provided with line array CCD image-forming assembly 11 (being the image-forming assembly that is made of lens combination, high sensitivity linear array CCD image sensor of the prior art) above electrophoresis tank, this assembly is connected with embedded computer system 12, and computer system connects LCD display 13.
Claims (4)
1. the dynamic imagery method of an agarose gel electrophoresis is characterized in that in the gel electrophoresis process, ultraviolet light is radiated on the gel, generates the band diagram picture by imageing sensor and computer real-time, and electrophoresis process and imaging process are carried out simultaneously.
2. the dynamic imagery method of agarose gel electrophoresis according to claim 1 is characterized in that, a ultraviolet light source is set below electrophoresis tank, and the correspondence position above electrophoresis tank, adorns a line array CCD image-forming assembly.
3. the imaging device in the dynamic imagery method of the described agarose gel electrophoresis of claim 1, it is characterized in that constituting of a whole set of imaging device: a shadow shield is arranged below the gel electrophoresis groove, below shadow shield, be provided with the ultraviolet light source of band snoot, the position corresponding with ultraviolet light source is provided with the line array CCD image-forming assembly above electrophoresis tank, this assembly is connected with an embedded computer system, and this computer system is connected to a built-in LCD display.
4. the dynamic imagery method of agarose gel electrophoresis according to claim 1 is characterized in that, in each imaging sampling of setting constantly, ultraviolet light source is once glittering, and image-forming assembly obtains the image information of horizontal delegation bands of a spectrum simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100586559A CN101308116A (en) | 2008-07-09 | 2008-07-09 | Agarose gel electrophoresis dynamic imaging method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100586559A CN101308116A (en) | 2008-07-09 | 2008-07-09 | Agarose gel electrophoresis dynamic imaging method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101308116A true CN101308116A (en) | 2008-11-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100586559A Pending CN101308116A (en) | 2008-07-09 | 2008-07-09 | Agarose gel electrophoresis dynamic imaging method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101308116A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012135849A1 (en) * | 2011-04-01 | 2012-10-04 | Life Technologies Corporation | Computer - controlled gel electrophoresis system |
| CN103713033A (en) * | 2013-12-31 | 2014-04-09 | 南京山诺生物科技有限公司 | Real-time, visible and image-shared nucleic acid electrophoresis device |
| CN105302553A (en) * | 2015-10-21 | 2016-02-03 | 北京工业大学 | VC++ based gel image identification and positioning method |
| CN107860813A (en) * | 2017-11-16 | 2018-03-30 | 上海仪龙生物科技有限公司 | A kind of PhastGel electrophoresis and real-time imaging devices |
-
2008
- 2008-07-09 CN CNA2008100586559A patent/CN101308116A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012135849A1 (en) * | 2011-04-01 | 2012-10-04 | Life Technologies Corporation | Computer - controlled gel electrophoresis system |
| CN103713033A (en) * | 2013-12-31 | 2014-04-09 | 南京山诺生物科技有限公司 | Real-time, visible and image-shared nucleic acid electrophoresis device |
| CN105302553A (en) * | 2015-10-21 | 2016-02-03 | 北京工业大学 | VC++ based gel image identification and positioning method |
| CN107860813A (en) * | 2017-11-16 | 2018-03-30 | 上海仪龙生物科技有限公司 | A kind of PhastGel electrophoresis and real-time imaging devices |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20081119 |