CN101306808A - Centrifugal separation process of carbon nano-tubes by density gradient method - Google Patents
Centrifugal separation process of carbon nano-tubes by density gradient method Download PDFInfo
- Publication number
- CN101306808A CN101306808A CNA2008100400329A CN200810040032A CN101306808A CN 101306808 A CN101306808 A CN 101306808A CN A2008100400329 A CNA2008100400329 A CN A2008100400329A CN 200810040032 A CN200810040032 A CN 200810040032A CN 101306808 A CN101306808 A CN 101306808A
- Authority
- CN
- China
- Prior art keywords
- solution
- gradient
- carbon nanotube
- carbon nano
- carbon nanotubes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Carbon And Carbon Compounds (AREA)
Abstract
The invention relates to a method for centrifugal separation of carbon nanotubes with a density gradient method. The method comprises the following steps: ultrasonically dispersing carbon nanotubes in an aqueous solution; preparing sucrose solutions with respective concentrations of 5%, 10%, 15%, 20% and 25%, according to mass percentage; sequentially and slowly adding a certain amount of sucrose solution into a same centrifuge tube to preparing a gradient solution; slowly dropping the ultrasonically dispersed carbon nanotube solution on the surface of the gradient solution, centrifuging with a horizontal rotor at 4,000rpm for 60min, and slowly sucking out the centrifuged mixed solution in equal two parts according to the percentages of solutions composing the gradient solution; and obtaining carbon nanotubes with different lengths. The inventive method has the advantage of simple process, can obtain carbon nanotubes with uniform length, and can be applied in the field of drug carriers.
Description
Technical field
The present invention relates to the method for a kind of method of separating carbon nano-tube, particularly a kind of centrifugal separation process of carbon nano-tubes by density gradient.
Background technology
Since 1991 and 1993 find multi-walled carbon nano-tubes and Single Walled Carbon Nanotube respectively, carbon nanotube (CNTs) has caused the extensive concern of each subject with the performance of the machinery of its excellence, electronics, physics, chemical aspect, be considered to have great application prospect, comprising electron device, engineering materials, catalysis, absorption-separation, memory device, electrode materials, biomedicine etc. at wide spectrum.
The production method of carbon current nanotube mainly contains arc discharge method, laser ablation method, chemical Vapor deposition process.The carbon nanotube quality of different batches production differs greatly, and as foreign matter content, carbon length of tube etc., this phenomenon is especially common in Single Walled Carbon Nanotube.The carbon nanotube acid treatment is to be in daily use and practical treatment process, but the phenomenon that carbon pipe range weak point differs also often appears in the pipe after handling, and it differs and can reach 10 times, even 100 times.
Carbon nanotube is applied to pharmaceutical carrier and is considered to a very promising field, and the carbon pipe that length differs greatly is applied to pharmaceutical carrier, must have the difference owing to the caused carrier band effect that differs of itself length.For the application of better biological aspect, it is very necessary obtaining length isostatic carbon nanotube, and in a sense, it is narrow more to wish to use size range, the carbon nanotube of weak point.
The method of separating the different lengths carbon nanotube now mainly contains gel electrophoresis and various chromatographic technique.And the nanotube of the weak point that often obtains has major diameter enrichment to a great extent, cause long thin short and thick phenomenon, and the time that needs is long, and isolating amount is few.
Summary of the invention
The purpose of this invention is to provide a kind of method that adopts density gradient method to separate the carbon nanotube of different lengths.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method of density gradient method separating carbon nano-tube is characterized in that the concrete steps of this method are;
A.. will be made into the aqueous solution that concentration is 0.5～1.5mg/ml with the nitration mixture processed carbon nanotubes, ultra-sonic dispersion fully disperses carbon nanotube;
B. sucrose is made into mass percent respectively and is 5%, 10%, 15%, 20%, 25% the aqueous solution, get isopyknic solution successively by the order from the high density to the lower concentration and be mixed with gradient solution;
C. lentamente in the gradient solution with step a gained dispersion liquid step b gained; The volume ratio of dispersion liquid and gradient solution is 1: 5;
D.4000rpm, horizontal rotor is centrifugal under the 60min condition;
E. according to the umber of gradient solution, with the slow sucking-off of mixed solution five equilibrium after centrifugal; Finally obtain the different carbon nanotube of length.
Above-mentioned carbon nanotube is multi-walled carbon nano-tubes or Single Walled Carbon Nanotube.
The method of density gradient centrifugation to be sample carry out in certain inertia gradient media (saccharose gradient) centrifugation or sedimentation equilibrium promptly is assigned to particle under certain centrifugal force in the gradient on some specific position, forms the not separation method of same district band.The advantage of this method is: 1. good separating effect can once obtain purer particle; 2. wide accommodation can separate the particle with factor in precipitation difference, can separate the particle that certain buoyant density is arranged again; 3. particle can not overstock distortion, can keep seed activity, and prevents that established district band is owing to convection current causes mixing.
The present invention adopts the method for density gradient centrifugation to come separating carbon nano-tube, makes finally can separate to obtain the continuous and narrower carbon nanotube of scope of size.The inventive method technology is simple, and is easy to operate, can realize suitability for industrialized production.
Description of drawings
Fig. 1 is the embodiment of the invention one a centrifugal back the superiors carbon nanotube electromicroscopic photograph.
Fig. 2 is the 3rd layer of carbon nanotube electromicroscopic photograph in the embodiment of the invention one centrifugal back.
Fig. 3 is the embodiment of the invention one a centrifugal back orlop carbon nanotube electromicroscopic photograph.
Embodiment
Embodiment one:
(1) at first, take by weighing 30mg through nitration mixture (V with electronic balance
The vitriol oil: V
Concentrated nitric acid=3: the 1) multi-walled carbon nano-tubes after the supersound process, be well-dispersed in the 30ml deionized water multi-walled carbon nano-tubes is ultrasonic, form the carbon nanotube dispersion liquid.
(2) make density gradient: taking by weighing 20g sucrose with electronic balance, to be made into mass percent respectively be 5%, 10%, 15%, 20%, 25% sucrose solution.Splash into centrifuge tube gently since 25% sucrose solution of getting 5ml successively, be mixed with gradient solution.
(3) the carbon nanotube dispersion liquid slowly is added on the gradient liquid.
(4) 4000rpm, horizontal rotor is centrifugal under the 60min condition.
(5) mixed solution after centrifugal is divided into 5 parts, with dropper slow sucking-off from the top.Finally obtain the different carbon nanotube of length.
Separating resulting is referring to Fig. 1,2,3.
Claims (2)
1. the method for a density gradient method separating carbon nano-tube is characterized in that the concrete steps of this method are:
A.. will be made into the aqueous solution that concentration is 0.5～1.5mg/ml with the nitration mixture processed carbon nanotubes, ultra-sonic dispersion fully disperses carbon nanotube;
B. sucrose is made into mass percent respectively and is 5%, 10%, 15%, 20%, 25% the aqueous solution, get isopyknic solution successively by the order from the high density to the lower concentration and be mixed with gradient solution;
C. lentamente in the gradient solution with step a gained dispersion liquid step b gained; The volume ratio of dispersion liquid and gradient solution is 1: 5;
D.4000rpm, horizontal rotor is centrifugal under the 60min condition;
E. according to the umber of gradient solution, with the slow sucking-off of mixed solution five equilibrium after centrifugal; Finally obtain the different carbon nanotube of length.
2. require the method for 1 described density gradient method separating carbon nano-tube according to the right profit, it is characterized in that described carbon nanotube is multi-walled carbon nano-tubes or Single Walled Carbon Nanotube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100400329A CN101306808B (en) | 2008-07-01 | 2008-07-01 | Centrifugal separation process of carbon nano-tubes by density gradient method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100400329A CN101306808B (en) | 2008-07-01 | 2008-07-01 | Centrifugal separation process of carbon nano-tubes by density gradient method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101306808A true CN101306808A (en) | 2008-11-19 |
| CN101306808B CN101306808B (en) | 2010-07-07 |
Family
ID=40123519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100400329A Expired - Fee Related CN101306808B (en) | 2008-07-01 | 2008-07-01 | Centrifugal separation process of carbon nano-tubes by density gradient method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101306808B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103086357A (en) * | 2013-02-21 | 2013-05-08 | 南昌航空大学 | Method for screening carbon nano tubes by using rotating electrophoresis |
| CN103088337A (en) * | 2013-01-31 | 2013-05-08 | 南昌航空大学 | Method for laser-induction hybrid cladding of copper composite coating dispersedly strengthened by carbon nanotubes (CNTs) |
| CN101712011B (en) * | 2009-11-13 | 2013-07-03 | 北京化工大学 | Method for centrifugally separating oil soluble nano particles by organic density gradient |
| WO2014015510A1 (en) * | 2012-07-27 | 2014-01-30 | 国家纳米科学中心 | Method for treating single wall carbon nanotube |
| CN111747399A (en) * | 2019-03-26 | 2020-10-09 | 中国科学院物理研究所 | Separation method of carbon nano tube |
-
2008
- 2008-07-01 CN CN2008100400329A patent/CN101306808B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101712011B (en) * | 2009-11-13 | 2013-07-03 | 北京化工大学 | Method for centrifugally separating oil soluble nano particles by organic density gradient |
| WO2014015510A1 (en) * | 2012-07-27 | 2014-01-30 | 国家纳米科学中心 | Method for treating single wall carbon nanotube |
| CN103088337A (en) * | 2013-01-31 | 2013-05-08 | 南昌航空大学 | Method for laser-induction hybrid cladding of copper composite coating dispersedly strengthened by carbon nanotubes (CNTs) |
| CN103088337B (en) * | 2013-01-31 | 2014-10-15 | 南昌航空大学 | Method for laser-induction hybrid cladding of copper composite coating dispersedly strengthened by carbon nanotubes (CNTs) |
| CN103086357A (en) * | 2013-02-21 | 2013-05-08 | 南昌航空大学 | Method for screening carbon nano tubes by using rotating electrophoresis |
| CN103086357B (en) * | 2013-02-21 | 2014-07-02 | 南昌航空大学 | Method for screening carbon nano tubes by using rotating electrophoresis |
| CN111747399A (en) * | 2019-03-26 | 2020-10-09 | 中国科学院物理研究所 | Separation method of carbon nano tube |
| CN111747399B (en) * | 2019-03-26 | 2021-11-23 | 中国科学院物理研究所 | Separation method of carbon nano tube |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101306808B (en) | 2010-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Oliveira et al. | New generation of electrochemical sensors based on multi-walled carbon nanotubes | |
| He et al. | Facile electrochemical sensor for nanomolar rutin detection based on magnetite nanoparticles and reduced graphene oxide decorated electrode | |
| Gogoi et al. | Enantiomeric recognition and separation by chiral nanoparticles | |
| CN101306808B (en) | Centrifugal separation process of carbon nano-tubes by density gradient method | |
| Martin et al. | The emerging field of nanotube biotechnology | |
| Chen et al. | Separation and characterization of single-walled and multiwalled carbon nanotubes by using flow field-flow fractionation | |
| Komatsu et al. | A comprehensive review on separation methods and techniques for single-walled carbon nanotubes | |
| Yu et al. | Determination of aflatoxin B1 and B2 in vegetable oils using Fe3O4/rGO magnetic solid phase extraction coupled with high-performance liquid chromatography fluorescence with post-column photochemical derivatization | |
| Shastry et al. | Probing carbon nanotube–surfactant interactions with two-dimensional DOSY NMR | |
| Nikoleli et al. | Structural characterization of graphene nanosheets for miniaturization of potentiometric urea lipid film based biosensors | |
| Yang et al. | Gold nanoparticals doping graphene sheets nanocomposites sensitized screen-printed carbon electrode as a disposable platform for voltammetric determination of guaiacol in bamboo juice | |
| Zou et al. | Vertically-ordered mesoporous silica films grown on boron nitride-graphene composite modified electrodes for rapid and sensitive detection of carbendazim in real samples | |
| Liu et al. | Preparative ultracentrifuge method for characterization of carbon nanotube dispersions | |
| Martín et al. | Multidimensional carbon allotropes as electrochemical detectors in capillary and microchip electrophoresis | |
| Ohmori et al. | Fractionation of single wall carbon nanotubes by length using cross flow filtration method | |
| Chatzimitakos et al. | Carbon-based nanomaterials functionalized with ionic liquids for microextraction in sample preparation | |
| Liu et al. | Breaking the nanoparticle’s dispersible limit via rotatable surface ligands | |
| Aldalbahi et al. | Conducting composite materials from the biopolymer kappa-carrageenan and carbon nanotubes | |
| Kang et al. | Selective growth of subnanometer diameter single-walled carbon nanotube arrays in hydrogen-free CVD | |
| Yang et al. | Highly‐sensitive Electrochemical Determination of Rutin Using NMP‐exfoliated Graphene Nanosheets‐modified Electrode | |
| Li et al. | Triazine‐based organic polymers@ SiO2 nanospheres for sensitive solid‐phase microextraction of polycyclic aromatic hydrocarbons | |
| Chen et al. | β-cyclodextrin-immobilized Ni/graphene electrode for electrochemical enantiorecognition of phenylalanine | |
| Kuang et al. | Ultrashort single-walled carbon nanotubes: Density gradient separation, optical property, and mathematical modeling study | |
| Drewniak et al. | Comparison of gas sensing properties of reduced graphene oxide obtained by two different methods | |
| Zhu et al. | Novel nanofluidic cells based on nanowires and nanotubes for advanced chemical and bio-sensing applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100707 Termination date: 20130701 |