CN101305916A - Ox blood serum acquisition method - Google Patents
Ox blood serum acquisition method Download PDFInfo
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- CN101305916A CN101305916A CNA2008100183774A CN200810018377A CN101305916A CN 101305916 A CN101305916 A CN 101305916A CN A2008100183774 A CNA2008100183774 A CN A2008100183774A CN 200810018377 A CN200810018377 A CN 200810018377A CN 101305916 A CN101305916 A CN 101305916A
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Abstract
The invention relates to a method for collecting animal serums, in particular to a method for collecting the ox serum. The method comprises the following steps: blood collecting, blood taking, centrifugation and split charging. The key points are as follows: sodium chloride injection of 0.9 percent is added in a blood taking bottle according to the proportion that the quantity of the sodium chloride injection is 1 percent of the total volume of the blood taking bottle, the blood taking bottle is put in a water bath case at 38 DEG C before the centrifugation, and the centrifugation is not executed until the serum begins to precipitate. The method has the advantages that the sodium chloride injection of 0.9 percent is added in the blood taking bottle, an isotonic environment is made artificially, the osmotic pressure of blood is not changed, and the hemolysis caused by erythrocyte rhexis cannot occur. The blood taking bottle is put in the water bath case at 38 DEG C to lead the blood to be heated uniformly; therefore, the precipitation of unstable protein is facilitated, the precipitation, the centrifugation and the separation of the serum is facilitated, and the collected serum has yellow appearance and is clear. The serum which is collected in the collecting method has good serum quality; in addition, the collecting quantity is 300ml much more than a traditional method. The income is increased by RMB30 to 60 from the serum of each ox, and the income increase of farmers and herders is promoted.
Description
Technical field
The present invention relates to a kind of animal serum acquisition method, more specifically to a kind of Ox blood serum acquisition method.
Background technology
Cell culture plays central role in the development of whole biotechnology industry, the development of cell culture, the quality of culture medium is again crucial, and the growth and breeding of animal serum pair cell is being brought into play important or even is difficult to alternate effect in the Main Ingredients and Appearance of culture medium.New-born calf serum is again the most widely in the application of animal serum, is one of raw material important in the medical biotechnology product.The market demand in 1 year of domestic new-born calf serum is more than 500,000 liters.A large amount of milch cows has been raised as the newborn meat base of China in provinces and regions such as the Inner Mongol of China, Heilungkiang, Hebei, Henan, Gansu, Ningxia, Xinjiang, owing to consider the economic benefit of cowboying, annual superseded a large amount of newborn stot.When these stots were butchered, blood can go out serum by aseptic collection, centrifugalize, is used to produce biological product such as vaccine, diagnostic reagent.Blood plasma is used to make the blood powder beverage of high protein.Since the bovine blood comprehensive utilization project implementation, local cattle valency is significantly improved, thereby greatly promoted increasing income of peasants and herdsmen, promoted local cattle-raising development, local expanding economy has been produced huge impetus, had important practical significance promoting local economy and social development.Calf blood directly was discharged in the sewer in the past, had not only reduced peasants and herdsmen's income, also environment had been caused certain pollution.At present; the way of domestic collection Ox blood serum is: scratch the calf skin of neck; after choosing the carotid artery sterilization; cut off an osculum with the operating scissors of the bacterium of going out, the sterile blood sampling pipe is inserted wherein, blood is flow in the centrifuge bottle by blood taking tube; again centrifuge bottle is put into centrifuge; with centrifugal about 30 minutes of 3600～4000 rotating speeds that change, shut down the back and take out centrifuge bottle, isolating serum is siphoned in the packing container with the sterile blood sampling pipe.The shortcoming of this collection serological method is: unstable fibrin is difficult for separating out, take in the serum finished product and separate out again, make finished product flocculent deposit occur, have influence on the outward appearance and the quality of finished product, thereby the user is thought by mistake pollute and dare not use, cause certain loss to enterprise.The fibrin of separating out is easy to stick on bottle wall, and serum is wrapped in wherein, is unfavorable for the centrifugalize of serum, can't be siphoned in the packing container, influences the collection quantity of serum.At this moment, must scratch fibrin with blade after, carry out centrifugal serum could the separation of the second time, when blade is scratched, be easy to following blood plasma is dug, erythrocyte easily breaks and causes haemolysis, makes the serum outward appearance red partially, directly has influence on the outward appearance of serum finished product.The centrifuge bottle that connects blood in addition is not to wait to ooze environment, because the change of osmotic pressure causes erythrocyte fragmentation and haemolysis easily.
Summary of the invention
The objective of the invention is provides a kind of blood serum acquisition method by traditional collection serum production technology is transformed, and improves the collection capacity of serum, improves the quality of serum.
The invention discloses a kind of Ox blood serum acquisition method, the step that following order is arranged: at first blood sampling: behind calf Baoding, wipe away the skin of neck sterilization with povidone iodine and 75% cotton ball soaked in alcohol sassafras, scratch the calf skin of neck with the disinfectant scalpel, and choose carotid artery vascular with the disinfectant mosquito forceps, clamp the carotid artery vascular two ends with two mosquito forcepss simultaneously, wipe away blood vessel with the cotton ball soaked in alcohol sassafras, reuse disinfectant operating scissors is cut off blood vessel one osculum in the middle of two mosquito forcepss, disposable blood taking tube is inserted, the other end of blood taking tube stretches into and connects in the blood bottle, is close to a bottle wall.Connect blood then: 0.9% sodium chloride injection is joined connecing in the blood bottle of the bacterium of going out in the ratio that connects blood bottle total measurement (volume) 1%, cover behind the bottle cap 45 ° of slowly rocking-turns that centrifuge bottle tilted, guarantee that injection soaks into all inwalls of centrifuge bottle.Take off the mosquito forceps by proximal part again, blood flows to through blood taking tube and connects the blood bottle.Connect the full bonnet upper bottle cover of blood bottle graft, be put into sequentially in 38 ℃ of water baths, observed once in per 10 minutes, observed once in per 5 minutes after half an hour, when serum begins to separate out, take out.Centrifugal again: as will to connect and put into centrifuge after the blood bottle takes out from water bath, centrifugal 25～30 minutes with 4000 rev/mins speed.Last packing: will connect the blood bottle after centrifuge is shut down and take out, the serum that will connect in the blood bottle with the sterile blood sampling pipe is siphoned in the packing container, fills after the sealing freezing immediately.
Advantage of the present invention is: add 0.9% sodium chloride injection in connecing the blood bottle, artificially made a grade and oozed environment, the osmotic pressure of blood can not change, can be because of erythrocyte fragmentation haemolysis.Connect the blood bottle in 38 ℃ of water baths, make the blood thermally equivalent, help separating out of labile protein like this, help the separating out of serum, centrifugal with separate, the serum outward appearance of collection is yellow and bright.The serum that the present invention gathers is the serum quality better not only, and collection quantity will have more more than 300 milliliters than traditional method.Calculate by 45 kilograms of calves, the multipotency of traditional method is gathered 1200 milliliters of serum, and the serum of collection of the present invention is 1500～1800 milliliters, and every Ox blood serum is overcharged benefit 30～60 circles, can promote increasing income of peasants and herdsmen.
The specific embodiment
Key of the present invention is: add 0.9% sodium chloride injection in the ratio that connects blood bottle total measurement (volume) 1% in connecing the blood bottle, will connect the blood bottle and be put in 38 ℃ of water baths before centrifugal, just can carry out centrifugal after serum begins to separate out.At first blood sampling: behind calf Baoding, wipe away the skin of neck sterilization with povidone iodine and 75% cotton ball soaked in alcohol sassafras, scratch the calf skin of neck with the disinfectant scalpel, and choose carotid artery vascular with the disinfectant mosquito forceps, and clamp the carotid artery vascular two ends with two mosquito forcepss simultaneously, wipe away blood vessel with the cotton ball soaked in alcohol sassafras, reuse disinfectant operating scissors is cut off blood vessel one osculum in the middle of two mosquito forcepss, disposable blood taking tube is inserted, and the other end of blood taking tube stretches into and connects in the blood bottle, is close to a bottle wall.Connect blood then: volumetrical the connecing of 1000 milliliters of the bacterium of will going out add 10 milliliter of 0.9% sodium chloride injection in the blood bottle, cover to connect the 45 ° of slowly rocking-turns of tilting of blood bottle behind the bottle cap, guarantee that 0.9% sodium chloride injection soaks into all inwalls that connect the blood bottle.Take off the mosquito forceps by proximal part again, blood flows to through blood taking tube and connects the blood bottle.Connect the full bonnet upper bottle cover of blood bottle graft, be put into sequentially in 38 ℃ of water baths, observed once in per 10 minutes, observed once in per 5 minutes after half an hour, when serum begins to separate out, take out.Centrifugal again: as will to connect and put into centrifuge after the blood bottle takes out from water bath, centrifugal 25～30 minutes with 4000 rev/mins speed.Last packing: will connect the blood bottle after centrifuge is shut down and take out, the serum that will connect in the blood bottle with the sterile blood sampling pipe is siphoned in the packing container, fills after the sealing freezing immediately.
Claims (1)
1, a kind of Ox blood serum acquisition method, it is characterized in that: this method has the step of following order;
A, blood sampling: behind calf Baoding, wipe away the skin of neck sterilization with povidone iodine and 75% cotton ball soaked in alcohol sassafras, scratch the calf skin of neck with the disinfectant scalpel, and choose carotid artery vascular with the disinfectant mosquito forceps, and clamp the carotid artery vascular two ends with two mosquito forcepss simultaneously, wipe away blood vessel with the cotton ball soaked in alcohol sassafras, reuse disinfectant operating scissors is cut off blood vessel one osculum in the middle of two mosquito forcepss, disposable blood taking tube is inserted, and the other end of blood taking tube stretches into and connects in the blood bottle, is close to a bottle wall;
B, connect blood: 0.9% sodium chloride injection is joined connecing in the blood bottle of the bacterium of going out in the ratio of the total measurement (volume) 1% that connects the blood bottle, cover behind the bottle cap 45 ° of slowly rocking-turns that centrifuge bottle tilted, guarantee that injection soaks into all inwalls of centrifuge bottle; Take off the mosquito forceps by proximal part then, blood flows to through blood taking tube and connects the blood bottle; Connect the full bonnet upper bottle cover of blood bottle graft, be put into sequentially in 38 ℃ of water baths, observed once in per 10 minutes, observed once in per 5 minutes after half an hour, when serum begins to separate out, take out;
C, centrifugal: will connect and put into centrifuge after the blood bottle takes out from water bath, centrifugal 25～30 minutes with 4000 rev/mins speed;
D, packing: will connect the blood bottle after centrifuge is shut down and take out, the serum that will connect in the blood bottle with the sterile blood sampling pipe is siphoned in the packing container, fills after the sealing freezing immediately.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100183774A CN101305916A (en) | 2008-05-27 | 2008-05-27 | Ox blood serum acquisition method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100183774A CN101305916A (en) | 2008-05-27 | 2008-05-27 | Ox blood serum acquisition method |
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| CN101305916A true CN101305916A (en) | 2008-11-19 |
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| CNA2008100183774A Pending CN101305916A (en) | 2008-05-27 | 2008-05-27 | Ox blood serum acquisition method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106383044A (en) * | 2016-08-31 | 2017-02-08 | 谭现花 | Serum separation method and equipment thereof |
| CN108770782A (en) * | 2018-06-14 | 2018-11-09 | 天津市正江现代生物技术有限公司 | A method of improving cow's serum quality |
| CN116099673A (en) * | 2023-04-13 | 2023-05-12 | 苏州双洳生物科技有限公司 | Intelligent blood collection system based on bovine serum acquisition |
-
2008
- 2008-05-27 CN CNA2008100183774A patent/CN101305916A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106383044A (en) * | 2016-08-31 | 2017-02-08 | 谭现花 | Serum separation method and equipment thereof |
| CN106383044B (en) * | 2016-08-31 | 2019-01-04 | 谭现花 | A kind of serum separation method and equipment |
| CN108770782A (en) * | 2018-06-14 | 2018-11-09 | 天津市正江现代生物技术有限公司 | A method of improving cow's serum quality |
| CN116099673A (en) * | 2023-04-13 | 2023-05-12 | 苏州双洳生物科技有限公司 | Intelligent blood collection system based on bovine serum acquisition |
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Open date: 20081119 |