CN101293869A - Quinoline and quinoxaline compounds which inhibit platelet-derived growth factor and/or p561lck tyrosine kinases - Google Patents

Quinoline and quinoxaline compounds which inhibit platelet-derived growth factor and/or p561lck tyrosine kinases Download PDF

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CN101293869A
CN101293869A CNA2008101005365A CN200810100536A CN101293869A CN 101293869 A CN101293869 A CN 101293869A CN A2008101005365 A CNA2008101005365 A CN A2008101005365A CN 200810100536 A CN200810100536 A CN 200810100536A CN 101293869 A CN101293869 A CN 101293869A
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quinoxaline
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dimethoxy
base
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M·R·迈尔斯
W·贺
A·P·斯帕达
M·P·马圭尔
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Aventis Pharmaceuticals Inc
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/36—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems
    • C07D241/38—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems with only hydrogen or carbon atoms directly attached to the ring nitrogen atoms
    • C07D241/40—Benzopyrazines
    • C07D241/44—Benzopyrazines with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the hetero ring
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/498—Pyrazines or piperazines ortho- and peri-condensed with carbocyclic ring systems, e.g. quinoxaline, phenazine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/20—Oxygen atoms
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/38—Nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
    • C07D241/36—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems
    • C07D241/38—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings condensed with carbocyclic rings or ring systems with only hydrogen or carbon atoms directly attached to the ring nitrogen atoms
    • C07D241/40—Benzopyrazines
    • C07D241/42—Benzopyrazines with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to carbon atoms of the hetero ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links

Abstract

This invention is directed to quinoline/quinoxaline compounds which inhibit platelet-derived growth factor or p56<lck> tyrosine kinase activity, to pharmaceutical compositions comprising these compounds, and to the use of these compounds for treating a patient suffering from or subject to disorders/conditions involving cellular differentiation, proliferation, extracellular matrix production or mediator release and/or T cell activation and proliferation.

Description

Suppress Thr6 PDGF BB and/or p56 LckThe quinoline of Tyrosylprotein kinase and quinoxaline compounds
The application is that application number is 98806430.8, the applying date is on May 28th, 1998, denomination of invention is for " suppressing Thr6 PDGF BB and/or p56 LckThe quinoline of Tyrosylprotein kinase and quinoxaline compounds " the dividing an application of Chinese patent application.
Technical field
The present invention relates to use quinoline/quinoxaline compounds and suppress the method that (chemotaxis) and/or t cell activation and propagation are moved in cell proliferation and/or cell matrix generation and/or cell, wherein compound used therefor is effective protein proteins tyrosine kinase inhibitor (TKI).
Background technology
Cell signaling is by comprising the system with interaction mediation of the outer acceptor of contact of cell-cells contacting or cell-matrix or born of the same parents-substrate contact.Extracellular signal is delivered to the other parts of cell usually through tyrosine kinase mediated phosphorylation event, wherein phosphorylation event can influence the substrate protein that conducts the mixture downstream with cytolemma bonded signal.Acceptor-the enzyme of one group-specific such as insulin receptor, EGF-R ELISA (EGF-R) or platelet derived growth factor receptor (PDGF-R) are the examples that participates in the Tyrosylprotein kinase of cell signaling.Effective enzyme-mediation phosphorylation for the substrate protein white matter that contains tyrosine residues needs the autophosphorylation effect of enzyme.Known these substrates can be responsible for the various kinds of cell incident, give some instances, and comprise cell proliferation, cell matrix generation, cell migration and apoptosis.
It is believed that the numerous disease state is by the excessive generation of uncontrolled breeding of cell or matrix or regulates and control due to the bad apoptosis.These morbid states relate to the broad variety cell, and comprise some illnesss like this, as restenosis or the fibroproliferative disease such as the sacroiliitis of leukemia, cancer, glioblastoma, psoriasis, diseases associated with inflammation, osteopathy, fibrosis disease, atherosclerosis and coronary artery, femoral artery, Renal artery postangioplasty, lung, kidney and the sex change of liver fibering.In addition, the non-control sexual cell proliferative disease due to the coronary bypass-forming operation.It is believed that excessive generation that suppresses uncontrolled breeding of tyrosine kinase activity pair cell or matrix or the control of the apoptosis that is difficult to regulate and control are very effective.
Same also known some tyrosinase inhibitor can with the tyrosine kinase interactions more than a type.Some Tyrosylprotein kinases are that to keep the human body normal function necessary.For example, under most of normal circumstancess, do not wish to suppress the effect of Regular Insulin.Therefore, for generating with cell proliferation and/or cell matrix and/or cell moves the selective therapy of the disease that (chemotaxis) be feature (as restenosis), can provide a kind of valuable medicament with the compound that is lower than effective inhibition insulin receptor kinase desired concn inhibition PDGF-R tyrosine kinase activity.
The present invention relates to adjust and/or suppress the method for cell signaling, cell proliferation, extracellular matrix generation, chemotaxis, also relate to the method that growth of control abnormity cell and cellular inflammation are replied.More specifically say, the present invention relates to the purposes of substituted quinoxaline compound, by suppressing Thr6 PDGF BB-acceptor (PDGF-R) tyrosine kinase activity and/or Lck tyrosine kinase activity effectively, these compounds discharge differentiation, propagation or medium and have selective inhibitory.
2. The report progress
All introduced in the lot of documents report tyrosine kinase receptor enzyme (as EGF-R or PDGF-R) or non-recipient cytoplasm Tyrosylprotein kinase (as v-abl, p56lck or c-src) have been had optionally tyrosine kinase inhibitor.Recently, (Exp.Opin.Ther.Patents 1995 for Spada and Myers (Exp.Opin.Ther.Patents 1995,5 (8), 805) and Bridges, 5 (12), 1245) in their summary, summarized the document of relevant tyrosine kinase inhibitor and EGF-R selective depressant respectively.In addition, Law and Lydon have also summarized anticancer effectiveness (the emerging medicine: improve the exploitation (Emerging Drugs:The Prospect For Improved Medicines) 1996 of medicine, 241-260) of tyrosine kinase inhibitor.
Known PRGF-R tyrosine kinase activity inhibitor comprise Maguire etc. ( The pharmaceutical chemistry magazine(J.Med.Chem.) 1994,37,2129) and Dolle etc. ( The pharmaceutical chemistry magazine1994,37,2627) Bao Dao quinolyl inhibitor.(EP 564409), Zimmerman such as recent Traxler, J.; And Traxler, P. etc. ( Biological organic and pharmaceutical chemistry communication(Biorg.﹠amp; Med.Chem.Lett.) 1996,6 (11), 1221-1226) and Buchdunger, E. etc. (Proc.Nat.Acad.Sci.1995,92,2558) have reported class phenyl amino-pyrimidin-4-yl-inhibitor.Although obtained these progress in this field, the medicament of being made by these species compound also is not approved for treatment human pcna disease.
Dependency between the multi-factor disease of restenosis and PDGF and the PDGF-R is confirmed in many scientific literatures already.Yet, relevant in recent years pnemnofibrosis disease (Antoniades, H.N. etc., Face Bed research magazine(J.Clin Invest.), 1990,86,1055), fibrotic conditions (Peterson, the T.C. of kidney and liver Hepatology(Hepatology), progress 1993,17,486) illustrates that also PDGF and PDGF-R play a role.For example, as Shultz etc. ( U.S.'s physiology magazine(Am.J.Physiol), 1988,255, F674) and Floege etc. ( Clinical and experiment immunization is learned(Clin.Exp.Immun.) 1991,86,334) described, glomerulonephritis be renal failure main diseases because of, and determined that PDGF is the external effective mitogen of mesangial cell.Thornton, S.C. etc. ( Clinical and experiment immunization is learned, 1991,86,79) reported TNF α-and PDGF (people provides by rheumatoid arthritis) be the major cytokine that participates in the propagation of synovial fluid cell.In addition, identified some specific tumor cells kinds (referring to Silver, B.J. Biotic factor(BioFactors), 1992,3,217), as glioblastoma and Kaposi, their overexpression PDGF albumen or its acceptor, thus cause cancer cells to pass through autocrine or the growth out of control of paracrine mechanism.Therefore, can expect, the PDGF tyrosine kinase inhibitor can be used for treating various come uncorrelated outwardly but on nosetiology all relevant human diseases with PDGF and/or PDGF-R.
Hanke etc. ( Inflammation research(Inflamm.Res.), 1995,44,357) and Bolen and Brugge ( Exempt from Epidemiology year summarizes(Ann.Rev.Immunol.), 1997,15,371) different non-receptor type Tyrosylprotein kinases such as p56 have all been commented Lck(hereinafter to be referred as " Lck ") effect in the inflammatory-related disorders that relates to t cell activation and propagation.These diseases associated with inflammation comprise allergy, autoimmune disease, rheumatoid arthritis and transplant rejection.Recently, another piece summary summarized comprise have Lck suppress active compound all kinds of tyrosine kinase inhibitors (Groundwater etc., The pharmaceutical chemistry progress(Progress inMedicinal Chemistry), 1996,33,233).Lck tyrosine kinase activity inhibitor comprises some natural products, and they are generally nonselective tyrosine kinase inhibitor, as Staurosporine, Sophoricol, some flavonoid and erbstatin.Recently also reported damnacanthol be Lck low nmole inhibitor (Faltynek etc., Biological chemistry(Biochemistry), 1995,34,12404).The example of synthetic Lck inhibitor comprises: a series of dihydroxyl-isoquinoline 99.9 inhibitor, it is reported they have low micromole to sub-micro mole activity (Burke etc., The pharmaceutical chemistry magazine, 1993,36,425); Quinoline (the IC of Lck lower with activity 50Be 610 micromoles).The researchist also disclose a series of 4-substituted quinazoline compounds that in micromole-sub-micro mole low strength range, can suppress Lck (Myers etc., WO95/15758 and Myers etc., Biological organic and pharmaceutical chemistry communication(Bioorg.Med.Chem.Lett.), 1997,7,417).The scientific research personnel of brightness end (Hanke etc., Journal of biological chemistry(J.Biol.Chem.) 1996,271,695) disclose two kinds of specificity pyrazolopyrimidine inhibitor that are called PP1 and PP2 respectively, they have the effect that is low to moderate the nmole level to Lck and Fyn (another kind of Src family kinases).About quinoline or quinoxalinyl compound, also do not report this Lck inhibitor on the one hand.Therefore, can expect, suppress the quinoline of Lck tyrosine kinase activity or quinoxaline inhibitors and can be used for treating and variously come uncorrelated outwardly but on nosetiology, all relate to the human diseases that the Lck tyrosine kinase signal conducts.
Summary of the invention
The present invention relates to formula I compound or its N-oxide compound, its hydrate, its solvate, its prodrug or its pharmacologically acceptable salt:
Figure A20081010053600151
Wherein:
X is L 1Or L 2Z 2
L 1Be (CR 3aR 3b) rOr (CR 3aR 3b) m-Z 3-(CR 3 ' aR 3 ' b) n
L 2Be (CR 3aR 3b) p-Z 4-(CR 3 ' aR 3 ' b) qOr vinylidene;
Z 1Be CH or N;
Z 2Be the optional cycloalkyl that replaces, the optional cycloalkenyl group that replaces, optional heterocyclic radical that replaces or the optional heterocycloalkenyl that replaces;
Z 3Be O, NR 4, S, SO or SO 2
Z 4Be O, NR 4, S, SO, SO 2Or valence link;
M is 0 or 1;
N is 2 or 3, and n+m=2 or 3;
P and q are 0,1,2,3 or 4 independently, and work as Z 4During for valence link, p+q=0,1,2,3 or 4, and work as Z 4When being not valence link, p+q=0 then, 1,2 or 3;
R is 2,3 or 4;
R 1aAnd R 1bBe the optional alkyl that replaces, the optional aryl that replaces, the optional heteroaryl that replaces independently, hydroxyl, acyloxy, the optional alkoxyl group that replaces, the optional cycloalkyloxy that replaces, the optional heterocyclic oxy group that replaces, the optional heterocycle carbonyl oxygen base that replaces, the optional aryloxy that replaces, the optional heteroaryloxy that replaces, cyano group, R 5R 6N-or acyl group R 5N-, perhaps R 1aAnd R 1bOne of be hydrogen or halogen, and another is the optional alkyl that replaces, the optional aryl that replaces, the optional heteroaryl that replaces, hydroxyl, acyloxy, the optional alkoxyl group that replaces, the optional cycloalkyloxy that replaces, the optional heterocyclic oxy group that replaces, the optional heterocycle carbonyl oxygen base that replaces, the optional aryloxy that replaces, the optional heteroaryloxy that replaces, cyano group, R 5R 6N-or acyl group R 5N-;
R 1cBe hydrogen, the optional alkyl that replaces, the optional aryl that replaces, the optional heteroaryl that replaces, hydroxyl, acyloxy, the optional alkoxyl group that replaces, the optional cycloalkyloxy that replaces, the optional heterocyclic oxy group that replaces, the optional aryloxy that replaces, the optional heteroaryloxy that replaces, halogen, cyano group, R 5R 6N-or acyl group R 5N-;
R 3a, R 3b, R 3 ' aAnd R 3 ' bBe hydrogen or alkyl independently;
R 4Be hydrogen, alkyl or acyl group; With
R 5And R 6Be hydrogen or alkyl, perhaps R independently 5And R 6Form azaheterocyclyl with the nitrogen-atoms that they connected.
Another aspect of the present invention relates to the formula I compound or pharmaceutically acceptable salt thereof that comprises medicine effective quantity and the pharmaceutical composition of pharmaceutically acceptable carrier.The invention still further relates to the intermediate that preparation I compound is used, the preparation method of intermediate and formula I compound, and formula I compound is used for the treatment of and suffers from or easily trouble and cytodifferentiation, propagation, extracellular matrix generate or medium release and/or t cell activation and propagation have the patient's of related disorders purposes.
Detailed Description Of The Invention
Except as otherwise noted, above reach the following term of using in the specification sheets full text of the present invention and should be understood to have following implication:
Definition:
" patient " not only comprises the people, but also comprises other Mammals.
" significant quantity " is meant and can effectively suppresses PDGF-R tyrosine kinase activity and/or Lck tyrosine kinase activity, thereby produce the amount of the The compounds of this invention of required result of treatment.
" alkyl " is meant to have about 1 side chain or linear aliphatic alkyl to about 10 carbon atoms.Preferred alkyl is to have about 1 " low alkyl group " to about 6 carbon atoms; Preferred alkyl has about 1 to about 4 carbon atoms.Branched one or more low alkyl groups such as methyl, ethyl or the propyl group of being meant is connected on the linear alkyl chain.Alkyl is alkoxy randomly, halogen, carboxyl, hydroxyl or R 5R 6N-replaces.The example of alkyl comprises methyl, methyl fluoride, difluoromethyl, trifluoromethyl, ethyl, n-propyl, sec.-propyl, butyl, sec-butyl, the tertiary butyl, amyl group and hexyl.
" alkenyl " is meant the aliphatic hydrocarbyl that contains carbon-to-carbon double bond, and this group can be the straight or branched group that contains about 10 carbon atoms of the 2-that has an appointment in its chain.Contain 2 to about 6 carbon atoms in the chain of preferred alkenyl; More preferably contain about 2 in the chain to about 4 carbon atoms.Branched one or more low alkyl groups such as methyl, ethyl or the propyl group of being meant is connected on the linear chain alkenylene chain." low-grade alkenyl " is meant and contains 2 groups to about 4 carbon atoms of having an appointment in the chain, and wherein said chain can be a straight or branched.Alkenyl can be replaced by carbalkoxy.Non-limiting examples of alkenyls comprises vinyl, propenyl, n-butene base, isobutenyl, 3-methyl but-2-ene base, positive pentenyl, heptenyl, octenyl, cyclohexyl butenyl and decene base.
" vinylidene " is meant-the CH=CH-group.
" cycloalkyl " be meant the non-aromaticity that contains about 10 carbon atoms of the 3-that has an appointment single-or encircle ring system more.As variable R 1a, R 1bOr R 1cThe cycloalkyl of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 following " naphthenic substituent " replaces: alkyl, hydroxyl, acyloxy, alkoxyl group, halogen, R 5R 6N-, acyl group R 5N-, carboxyl or R 5R 6The NCO-substituting group, or (O-), more preferred substituents is an alkyl, hydroxyl, acyloxy, alkoxyl group, divalence oxygen and R to be positioned at the divalence oxygen that forms epoxide on two adjacent carbonss 5R 6NCO-.As variable Z 2The cycloalkyl of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 following " naphthenic substituent " replaces: alkyl, alkoxyl group, halogen, R 5R 6N-, acyl group R 5N-, carboxyl or R 5R 6The NCO-substituting group, or (O-), more preferred substituents is an alkyl, hydroxyl, acyloxy, alkoxyl group, divalence oxygen and R to be positioned at the divalence oxygen that forms epoxide on two adjacent carbonss 5R 6NCO-.And when cycloalkyl was replaced by at least two hydroxyl substituents, then at least two hydroxyl substituents can form corresponding ketal or acetal with aldehydes or ketones ketalization that contains 1-6 carbon atom or acetalation.The ketalization of gem-glycol causes forming spiral shell condensed ring system.Preferred spirocyclane basic ring is 1,4-dioxo spiro [4,5] last of the ten Heavenly stems-8-base.The preferred monocyclic cycloalkyl ring that does not replace or replace comprises cyclopentyl, fluoro cyclopentyl, cyclohexyl, and suberyl; More preferably cyclohexyl and cyclopentyl.The example of many ring cyclopentyl rings comprises the 1-naphthane, diamantane-(1-or 2-) base, [2.2.1] two suberyl (norcamphyl) and [2.2.2] two ring octyl groups; More preferably [2.2.1] two suberyl and [2.2.2.] two encircle octyl groups.
" cycloalkenyl group " is meant the non-aromatic monocycle that contains carbon-to-carbon double bond and about 10 carbon atoms of about 3-or encircles ring system more.As variable R 1a, R 1bOr R 1cThe cycloalkenyl group of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 above-mentioned naphthenic substituent replaces.As variable Z 2The cycloalkenyl group of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 above-mentioned naphthenic substituent replaces.The preferred monocycle cyclenes basic ring that does not replace or replace comprises cyclopentenyl, cyclohexenyl and cycloheptenyl; More preferably cyclopentenyl and cyclohexenyl.Preferred many ring cyclenes basic rings comprise [2.2.1] bicycloheptene base (norbornene) and [2.2.2] bicyclooctene base.
" aryl " is meant the aromatic carbon ring group that contains about 10 carbon atoms of the 6-that has an appointment.Typical aryl comprises phenyl or naphthyl, or by the phenyl or naphthyl of one or more aromatic yl group substituting group replacements that can be identical or different, wherein " aromatic yl group substituting group " comprises hydrogen, hydroxyl, halogen, alkyl, alkoxyl group, carboxyl, carbalkoxy or Y 1Y 2NCO-, wherein Y 1And Y 2Be hydrogen or alkyl independently.Preferred aryl groups group substituting group comprises hydrogen, halogen and alkoxyl group.
" heteroaryl " is meant about 5-to about 10-fragrant monocycle of unit or polynuclear hydrocarbon ring system, and wherein the one or more carbon atoms in the ring system are non-carbon, as nitrogen, and oxygen or sulphur.At the heteroaryl previous crops is that azepine, oxa-or the thia name expression of prefix exists at least one nitrogen respectively, and oxygen or sulphur atom are as annular atoms." heteroaryl " also can be replaced by one or more above-mentioned " aromatic yl group substituting group ".Typical heteroaryl comprises replacement pyrazinyl, furyl, thienyl, pyridyl, pyrimidyl ， isoxazolyl, isothiazolyl oxazolyl, thiazolyl, pyrazolyl, the furazan base, pyrryl, imidazo [2,1-b] thiazolyl, benzo furazan base, indyl, azaindolyl, benzimidazolyl-, benzothienyl, quinolyl, imidazolyl and isoquinolyl.
" heterocyclic radical " is meant the about 10 yuan of monocycles of about 4-or encircles ring system more, and wherein one or more ring system atoms are the non-carbon atom that is selected from nitrogen, oxygen or sulphur.As variable R 1a, R 1bOr R 1cThe heterocyclic radical of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 above-mentioned naphthenic substituent replaces.As variable Z 2The heterocyclic radical of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 above-mentioned naphthenic substituent replaces.At the heterocyclic radical previous crops is that azepine, oxa-or the thia name expression of prefix exists at least one nitrogen respectively, and oxygen or sulphur atom are as annular atoms.Typical monocyclic heterocycles base comprises piperidyl, pyrrolidyl, piperazinyl, morpholinyl, parathiazan base, thiazolidyl, 1,3-dioxolanyl, 1,4-alkyl dioxin, tetrahydrofuran base, tetrahydro-thienyl, tetrahydro thiapyran base etc.Typical heterocyclic radical partly comprises quinuclidinyl, and oneself encircles sulphur, THP trtrahydropyranyl, tetrahydro-thienyl, pyrrolidyl, tetrahydrofuran base, 7-oxabicyclo [2.2.1] heptyl or 4-piperidino-(1-position only) piperidines.
" heterocycle carbonyl oxygen base " is meant heterocyclic radical-C (O) O-group, and heterocyclic radical wherein as above defines.Representational heterocycle carbonyl oxygen base be [1,4 ']-Lian piperidyl-1 '-Ji carbonyl oxygen base (4-piperidino-(1-position only) piperidines-1-base carbonyl oxygen base).
" heterocycloalkenyl " is meant the about 10 yuan of monocycles of about 4-of fractional saturation or encircles ring system more, and one or more atoms are the non-carbon atom that is selected from nitrogen, oxygen or sulphur in the ring system.As variable R 1a, R 1bOr R 1cThe heterocycloalkenyl of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 above-mentioned naphthenic substituent replaces.As variable Z 2The heterocycloalkenyl of a part is randomly by one or more, and preferred 1-3, more preferably 1-2 above-mentioned naphthenic substituent replaces.At the heterocycloalkenyl previous crops is that azepine, oxa-or the thia name expression of prefix exists at least one nitrogen respectively, and oxygen or sulphur atom are as annular atoms.Typical monocycle azepine heterocycloalkenyl comprises 1,2,3,4-tetrahydro pyridyl, 1,2-dihydropyridine base, 1,4-dihydropyridine base, 1,2,3,6-tetrahydro pyridyl, 1,4,5,6-tetrahydro-pyrimidine base, 2-pyrrolinyl, 3-pyrrolinyl, 2-imidazolinyl, 2-pyrazolinyl etc.Representational oxa-heterocycloalkenyl comprises 3,4-dihydro-2H-pyrans, dihydrofuran base and fluoro dihydrofuran base.The assorted heterocycloalkenyl of representational many epoxies is 7-oxabicyclo [2.2.1] heptenyl.Representational monocycle thia heterocycloalkenyl comprises dihydro-thiophene base and dihydro thiapyran base.
" acyl group " is meant H-CO-or alkyl-CO-group, and alkyl wherein as above defines.Preferred acyl group comprises low alkyl group.Representational acyl group comprises formyl radical, ethanoyl, propionyl, 2-methylpropionyl, butyryl radicals and caproyl.
" aroyl " is meant aryl-CO-group, and aryl wherein as above defines.Representative groups comprises benzoyl and 1-and 2-naphthoyl.
" alkoxyl group " is meant alkyl-O-group, and alkyl wherein as above defines.Preferred alkoxyl group is " lower alkoxy " with about 6 carbon atoms of about 1-.Alkoxyl group can randomly be replaced by one or more amino, alkoxyl group, carboxyl, carbalkoxy, carboxyl aryl, formamyl or heterocyclic radical group.Representational alkoxyl group comprises methoxyl group, oxyethyl group, positive propoxy; isopropoxy, n-butoxy, heptan the oxygen base; 2-(morpholine-4-yl) oxyethyl group; 2-(oxyethyl group) oxyethyl group, 2-(4-methylpiperazine-1-yl) oxyethyl group, formamyl; N-methylamino formyl radical; N, N-formyl-dimethylamino, carboxyl methoxyl group and methoxycarbonyl methoxyl group.
" cycloalkyloxy " is meant cycloalkyl-O-group, and wherein cycloalkyl as mentioned above.Exemplary cycloalkyloxy comprises cyclopentyloxy and cyclohexyloxy.
" heterocyclic oxy group " is meant heterocyclic radical-O-group, and heterocyclic radical wherein as mentioned above.Exemplary heterocyclic oxy group group comprises the quinine epoxy group(ing), the own epoxy group(ing) of sulphur, tetrahydro-pyran oxy, tetramethylene sulfide oxygen base, pyrroles's alkoxyl group, tetrahydrofuran oxygen base and 7-oxabicyclo [2.2.1] oxygen in heptan base.
" aryloxy " is meant aryl-O-group, and wherein aryl as mentioned above.
" heteroaryloxy " is meant heteroaryl-O-group, and wherein heteroaryl as mentioned above.
" acyloxy " is meant acyl group-O-group, and wherein acyl group as mentioned above.
" carboxyl " is meant HO (O) C-(carboxylic acid) base.
" R 5R 6N-" be meant and replace or unsubstituted amino, wherein R 5And R 6As mentioned above.Typical case's group comprises amino (H 2N-), methylamino-, ethylmethylamino, dimethylamino and diethylamino.
" R 5R 6NCO-" be meant and replace or unsubstituted formamyl, wherein R 5And R 6As mentioned above.Typical case's group comprises formamyl (H 2NCO-), N-methylamino formyl radical (MeNHCO-) and N, N-formyl-dimethylamino (Me 2NCO-).
" acyl group R 5N-" be meant amido, wherein R 5As above define with acyl group.
" halogen " is meant fluorine, chlorine, bromine, or iodine.Preferred fluorine, chlorine or bromine, more preferably fluorine or chlorine.
" prodrug " is meant and is fit to be applied to the patient and do not have effects such as too high toxicity, pungency, atopic reaction, can be effectively used to the formula I compound form of intended purpose simultaneously again, comprises ketal, ester and zwitterionic form.Prodrug in vivo by for example in blood hydrolysis be converted into the following formula parent compound.Detailed description is seen T.Higuchi and V.Stella, Prodrug as novel drug delivery system, A.C.S.Symposium Series, the 14th volume, and Edwrd B.Roche etc. In the medicinal design Biological reversible carrier, American Pharmaceutical Association and Pergamon Press, 1987, the content of these two pieces of documents is incorporated into incorporated by reference at this.
" solvate " is meant the physics associated complex of The compounds of this invention and one or more solvent molecules.The ionic and covalent bonding that relates to variable pitch in this physics associated complex closes, and comprises hydrogen bonding.In some cases, solvate can separate, and is like this when one or more solvent molecules are attached in the lattice of crystalline solid for example." solvate " comprises solution phase and separable solvate.Typical solvate comprises ethylate, methylate etc." hydrate " is meant that wherein solvent molecule is the solvate of water.
Embodiment preferred
On the one hand, the preferred compound of the present invention is a formula I compound, or its N-oxide compound, its hydrate, its solvate, its prodrug or its pharmacologically acceptable salt, wherein:
L 1Be (CR 3aR 3b) m-Z 3-(CR 3 ' aR 3 ' b) n
L 2Be (CR 3aR 3b) p-Z 4-(CR 3 ' aR 3 ' b) q
Z 2Be the optional cycloalkyl that replaces, optional cycloalkenyl group that replaces or the optional heterocyclic radical that replaces;
Z 4Be O and NR 4
M is 0;
N is 2 or 3,
P+q=0 or 1;
R 1aAnd R 1bBe the optional alkyl that replaces, the optional alkoxyl group that replaces, the optional cycloalkyloxy that replaces, optional heterocyclic oxy group or the R that replaces independently 5R 6N-, perhaps R 1aAnd R 1bOne of be hydrogen or halogen, and another is the optional alkyl that replaces, the optional alkoxyl group that replaces, the optional cycloalkyloxy that replaces, optional heterocyclic oxy group or the R that replaces 5R 6N-;
R 1cBe hydrogen, optional alkyl that replaces or the optional alkoxyl group that replaces;
R 3a, R 3b, R 3 ' aAnd R 3 ' bBe hydrogen or low alkyl group independently;
R 4Be hydrogen; With
R 5And R 6Form azaheterocyclyl with the nitrogen-atoms that they connected.
On the other hand, the preferred compound of the present invention is a formula I compound, or its N-oxide compound, its hydrate, its solvate, its prodrug or its pharmacologically acceptable salt, wherein:
X is L 2Z 2
L 2Be (CR 3aR 3b) p-Z 4-(CR 3 ' aR 3 ' b) q
Z 2Be optional cycloalkyl that replaces or the optional cycloalkenyl group that replaces;
Z 3Be O and NR 4
P is 0;
Q is 0 or 1;
R 1aAnd R 1bBe the optional alkyl that replaces independently, the optional alkoxyl group that replaces, the optional cycloalkyloxy that replaces or the optional heterocyclic oxy group that replaces, perhaps R 1aAnd R 1bOne of be hydrogen or halogen,
R 1cBe hydrogen;
R 3 ' aAnd R 3 ' bBe hydrogen independently; With
R 4Be hydrogen.
On the other hand, the preferred compound of the present invention is L wherein 1Formula I compound for low alkyl group.
On the other hand, the preferred compound of the present invention is Z wherein 1Formula I compound for CH.
On the other hand, the preferred compound of the present invention is Z wherein 1Formula I compound for N.
On the other hand, the preferred compound of the present invention is Z wherein 2Formula I compound for the optional cycloalkyl that replaces.
On the other hand, the preferred compound of the present invention is Z wherein 2The formula I compound of the monocyclic cycloalkyl (more preferably methylcyclopentyl or methylcyclohexyl) that replaces for alkyl.
On the other hand, the preferred compound of the present invention is Z wherein 2Formula I compound for polycyclic naphthene base (more preferably [2.2.1] two suberyl (norcamphyl) and [2.2.1] two ring octyl groups).
On the other hand, the preferred compound of the present invention is Z wherein 2Formula I compound for the optional cycloalkenyl group (more preferably cyclopentenyl and cyclohexenyl) that replaces.Preferred many ring cyclenes basic rings comprise [2.2.1] bicycloheptene base (norbornene) and [2.2.2] bicyclooctene base.
On the other hand, the preferred compound of the present invention is Z wherein 2Formula I compound for cyclopentenyl or cyclohexenyl.
On the other hand, the preferred compound of the present invention is Z wherein 2Formula I compound for many ring cycloalkenyl groups (more preferably [2.2.1] bicycloheptene base (norbornene) or [2.2.2] bicyclooctene base).
On the other hand, the preferred compound of the present invention is that wherein p and q are 0 formula I compound.
On the other hand, the preferred compound of the present invention is the formula I compound of wherein p+q=1.
On the other hand, the preferred compound of the present invention is Z wherein 4Formula I compound for O.
On the other hand, the preferred compound of the present invention is Z wherein 4Be O, and p and q are 0 formula I compound.
On the other hand, the preferred compound of the present invention is Z wherein 4Be O, and the formula I compound of p+q=1.
On the other hand, the preferred compound of the present invention is Z wherein 4Be NR 4Formula I compound.
On the other hand, the preferred compound of the present invention is Z wherein 4Be NR 4, and p and q are 0 formula I compound.
On the other hand, the preferred compound of the present invention is Z wherein 4Be NR 4, and the formula I compound of m+n=1.
On the other hand, the preferred compound of the present invention is Z wherein 4Formula I compound for S.
On the other hand, the preferred compound of the present invention is Z wherein 4Be S, and p and q are 0 formula I compound.
On the other hand, the preferred compound of the present invention is Z wherein 4Be S, and the formula I compound of p+q=1.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bBe the optional low alkyl group that replaces of hydroxyl, hydroxyl, lower alkoxy, cycloalkyloxy, heterocyclic oxy group, perhaps R independently 1aAnd R 1bOne of be hydrogen or halogen.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bBe heterocyclic radical carbonyl oxygen base or the optional lower alkoxy that replaces independently; More preferably, lower alkoxy is a methoxy or ethoxy.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bBe low alkyl group, preferred low alkyl group is methyl or ethyl.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bOne of be lower alkoxy, and R 1aAnd R 1bIn another be halogen; Preferred lower alkoxy is a methoxy or ethoxy, and halogen is a chlorine or bromine.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bOne of be low alkyl group, and R 1aAnd R 1bIn another be lower alkoxy; Preferred lower alkoxy is a methoxy or ethoxy, and low alkyl group is methyl or ethyl.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bOne of be lower alkoxy, and R 1aAnd R 1bIn another be cycloalkyloxy; Preferred lower alkoxy is a methoxy or ethoxy, and cycloalkyloxy is cyclopentyloxy or cyclohexyloxy.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bOne of be hydrogen, and R 1aAnd R 1bIn another be lower alkoxy, cycloalkyloxy or heterocyclic oxy group; More preferably, lower alkoxy is a methoxy or ethoxy, and cycloalkyloxy is cyclopentyloxy or cyclohexyloxy, and heterocyclic oxy group is a furans oxygen base.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1cBe hydrogen, low alkyl group or lower alkoxy; More preferably, lower alkoxy is a methoxy or ethoxy.
On the other hand, the preferred compound of the present invention is formula I compound, wherein Z 2Be the hydroxyl cycloalkyl that (hydroxyl or alkyl) replaces, more preferably (low alkyl group) hydroxyl cycloalkyl of replacing.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bOne of be lower alkoxy, lower alkoxy wherein is alkoxy randomly, heterocyclic radical, carboxyl, carbalkoxy or formamyl replace.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bOne of be unsubstituted lower alkoxy, and R 1aAnd R 1bIn another be the lower alkoxy that alkoxy, heterocyclic radical, carboxyl, carbalkoxy or formamyl replace.
On the other hand, the preferred compound of the present invention is formula I compound, wherein R 1aAnd R 1bOne of be methoxyl group, and R 1aAnd R 1bIn another be [1,4 ']-Lian piperidines-1 '-Ji carbonyl oxygen base, 2-(oxyethyl group) oxyethyl group, 2-(4-morpholinyl) oxyethyl group, 2-(4-methylpiperazine-1-yl) oxyethyl group, carboxyl methoxyl group, the methoxycarbonyl methoxyl group, the aminocarboxyl methoxyl group, N-methylamino carbonyl methoxyl group or N, N-dimethylamino carbonyl methoxyl group.
The preferred compound of the present invention is selected from following material:
3-cyclohexyloxy-6, the 7-dimethoxy-quinoline;
2-cyclohexyl amino-6,7-dimethoxy quinoxaline;
Outward-two the ring [2.2.1] heptan-2-base-(6-chloro-7-methoxyl group quinoxaline-2-yl) amine;
Outward-two the ring [2.2.1] heptan-2-base-(7-chloro-6-methoxyl group quinoxaline-2-yl) amine;
Two the ring [2.2.1] heptan-2-base-(6,7-dimethyl quinoxaline-2-yl) amine;
2-suberyl amino-6,7-dimethoxy quinoxaline;
2-cyclopentyl amino-6,7-dimethoxy quinoxaline;
2-cyclohexyl amino-6-methoxyl group quinoxaline;
3-aminocyclohexyl-6, the 7-dimethoxy-quinoline;
(6,7-dimethoxy yl-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base) amine;
2-cyclohexyl amino-6-methoxyl group-7-bromo-quinoxaline hydrochloride;
(6,7-dimethoxy-quinoline-3-yl)-cis/trans-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-trans-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-(3-methyl-cyclopentyl)-amine;
Hexamethylene-3-thiazolinyl-(6,7-dimethoxy quinoxaline-2-yl)-amine;
2,7-two-cyclohexyloxy-6-methoxyl group-quinoxaline;
Cyclohexyl-(6,7-dimethoxy quinoxaline-2-ylmethyl)-amine;
(6,7-dimethoxy-quinoline-3-yl)-isobutylamine;
Cyclohexyl-(6-methoxyl group-7-morpholine-4-base-quinoxaline-2-yl)-amine;
(±)-two ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
Outward-two the ring [2.2.1] heptan-5-alkene-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
Cyclohexyl-(6,8-dimethyl-quinoxaline-2-yl)-amine;
In-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
(6,7-dimethoxy quinoxaline-2-yl)-(4-methoxyl group-cyclohexyl)-amine;
Outward-two the ring [2.2.1] heptan-2-base-(6-methoxyl group quinoxaline-2-yl)-amine;
Outward-and 2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy quinoxaline;
2-(two ring [2.2.2] sufferings-2-base oxygen base)-6,7-dimethoxy-quinoxaline;
In-2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy-quinoxaline;
Outward-and 2-(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline;
2-(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline;
2-cyclohexyloxy-6,7-dimethoxy quinoxaline;
2-encircles penta sulfenyl-6,7-dimethoxy quinoxaline;
6,7-dimethoxy-2-cyclopentyloxy-quinoxaline;
2-cyclopentyl methoxyl group-6,7-dimethoxy quinoxaline;
6,7-dimethoxy-2-tetrahydropyrans-4-oxygen base-quinoxaline;
Outward, outer-6,7-dimethoxy-2-(5,6-epoxy-two ring [2.2.1] heptane-2-base oxygen base) quinoxaline;
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base oxygen base)-hexahydrobenzoic acid;
6,7-dimethoxy-2-(4-methoxyl group-cyclohexyloxy)-quinoxaline;
3-cyclohexyloxy-6,7-dimethoxy quinoxaline 1-oxide compound;
(1R, 2R, 4S)-(+)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
(1S, 2S, 4R)-(-)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
2-(6,7-dimethoxy-quinoxaline-2-yl)-2-aza-bicyclo [2.2.2] octane-3-ketone;
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
Cis/trans-4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexahydrobenzoic acid;
Cis-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
Trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
(6,7-dimethoxy-quinoxaline-2-yl)-cis/trans-(3-(R)-methylcyclohexyl) amine;
(6,7-dimethoxy-quinoxaline-2-yl)-trans-(3-(R)-methylcyclohexyl) amine;
(6,7-dimethoxy-quinoxaline-2-yl)-cis-(3-(R)-methylcyclohexyl) amine; With
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base oxygen base)-hexahydrobenzoic acid methyl esters, or
Its N-oxide compound, its hydrate, its solvate, its prodrug, or its pharmacologically acceptable salt.
Preferred compound is a following compounds:
2-cyclohexyl amino-6,7-dimethoxy quinoxaline;
Outward-two the ring [2.2.1] heptan-2-base-(6-chloro-7-methoxyl group quinoxaline-2-yl) amine;
Outward-two the ring [2.2.1] heptan-2-base-(7-chloro-6-methoxyl group quinoxaline-2-yl) amine;
Two the ring [2.2.1] heptan-2-base-(6,7-dimethyl quinoxaline-2-yl) amine;
2-suberyl amino-6,7-dimethoxy quinoxaline;
2-cyclopentyl amino-6,7-dimethoxy quinoxaline;
3-aminocyclohexyl-6, the 7-dimethoxy-quinoline;
(6,7-dimethoxy yl-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base) amine;
(6,7-dimethoxy-quinoline-3-yl)-cis/trans-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-trans-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base)-amine;
Hexamethylene-3-thiazolinyl-(6,7-dimethoxy quinoxaline-2-yl)-amine;
2,7-two-cyclohexyloxy-6-methoxyl group-quinoxaline;
(6,7-dimethoxy-quinoline-3-yl)-isobutylamine;
(±)-two ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
Outward-two the ring [2.2.1] heptan-5-alkene-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
In-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
Outward-two the ring [2.2.1] heptan-2-base-(6-methoxyl group quinoxaline-2-yl)-amine;
Outward-and 2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy quinoxaline;
2-(two ring [2.2.2] sufferings-2-base oxygen base)-6,7-dimethoxy-quinoxaline;
In-2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy-quinoxaline;
Outward-and 2-(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline;
2-(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline;
2-cyclohexyloxy-6,7-dimethoxy quinoxaline;
2-encircles penta sulfenyl-6,7-dimethoxy quinoxaline;
6,7-dimethoxy-2-cyclopentyloxy-quinoxaline;
2-cyclopentyl methoxyl group-6,7-dimethoxy quinoxaline;
6,7-dimethoxy-2-tetrahydropyrans-4-oxygen base-quinoxaline;
Outward, outer-6,7-dimethoxy-2-(5,6-epoxy-two ring [2.2.1] heptane-2-base oxygen base) quinoxaline;
6,7-dimethoxy-2-(4-methoxyl group-cyclohexyloxy)-quinoxaline;
(1R, 2R, 4S)-(+)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
(1S, 2S, 4R)-(-)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
Cis-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
Trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
(6,7-dimethoxy-quinoxaline-2-yl)-cis/trans-(3-(R)-methylcyclohexyl) amine;
(6,7-dimethoxy-quinoxaline-2-yl)-trans-(3-(R)-methylcyclohexyl) amine;
(6,7-dimethoxy-quinoxaline-2-yl)-cis-(3-(R)-methylcyclohexyl) amine; With
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base oxygen base)-hexahydrobenzoic acid methyl esters, or
Its N-oxide compound, its hydrate, its solvate, its prodrug, or its pharmacologically acceptable salt.
Should be appreciated that all suitable combinations that the present invention includes concrete and preferred group described here.
The compounds of this invention can adopt known method in the document by the known compound or the easy intermediate preparation of preparation.Exemplary general method is as follows.
In addition, also can preparation I compound according to following reaction process I-VIII, wherein each variable as mentioned above, but it will be apparent to those skilled in the art that those and the inconsistent variable of described method should except.
Reaction process I
Reaction process II
Figure A20081010053600282
Reaction process III
Figure A20081010053600291
Reaction process IV
Figure A20081010053600292
Reaction process V
Reaction process VI
Figure A20081010053600294
R wherein 1a, R 1bAnd R 1cIn at least one is rudimentary wherein R 1a, R 1bAnd R 1cIn at least one as defined herein,
Alkoxyl group, and X " ' be L 1OP ' or L 2Z 2, wherein and when X be L 1In OP ' time, remove protecting group then
P ' protects P ' for being suitable in the presence of alkali and alkylating agent, obtain corresponding OH group
The blocking group of oh group
In reaction process VI, VII and VIII, the R that the R representative is defined herein 1a, R 1bOr R 1cThe precursor group of group, so that work as RBr, when reacting, ROH or RCOCl and aromatic hydroxy group form R under the condition described in reaction process VI, VII and the VIII 1a, R 1bOr R 1c
Representational RBr comprises the methyl and the ethyl ester of bromoacetic acid and bromoacetic acid.
Representational ROH comprises cellosolvo, 2-(4-morpholinyl) ethanol and 3-(4-methylpiperazine base) propyl alcohol.
Representational RCOCl be [1,4 ']-Lian piperidines-1 '-Ji carbonyl chlorine.
Reaction process VII
X wherein " ' be L 1OP " or L 2Z 2, wherein P is " for being suitable for the group of protection hydroxyl under reaction conditions described in reaction process I, II, III and the IX
X wherein " ' be L 1OP " or L 2Z 2, wherein P " and " ' for being suitable under reaction conditions described in reaction process I, II, III and the IX group of protection hydroxyl
Figure A20081010053600312
Reaction process IX
Figure A20081010053600321
X aBe Cl, Br or I
X " ' be (L 1OP ' or L 2Z 2),
Wherein P ' is for being suitable at Grignard reagent as X " ' be L 1OP ' time, then OP '
Existing down, the group of protection hydroxylic moiety partly can utilize suitable deprotection agent
Be converted into corresponding oh group
Reaction process X
Figure A20081010053600322
I. general method:
1. coupling 2-chlorine substituted quinoxaline and amine or aniline
Under about 160 ℃-about 180 ℃, heating 2-chloro-6, about 3 hours of the mixture of 7-dimethoxy quinoxaline (1 equivalent) and amine (about 5 equivalents of about 1-) is to spending the night.The burgundy residue is dissolved in ethanol/methylene (0%-10%), and silica gel column chromatography with hexane/ethyl acetate or ethanol/methylene (0%-100%) wash-out, obtains required product.Required product can be further by recrystallization purifying in methyl alcohol, methylene dichloride or methanol.
2. coupling 2-chlorine substituted quinoxaline and alcohol or phenol
With alcohol or mercaptan (1 equivalent) and the suspension returning of sodium hydride (about 3 equivalents of about 1-) in dry DMF/THF (0%-50%) 1 hour, add 2-chloro-6 then, 7-dimethoxy quinoxaline (1 equivalent).Backflow gained mixture about 1-4 hour.In and suspension to about pH 5-8, and be distributed among methylene dichloride and the salt solution.Residue behind the concentrated methylene dichloride is carried out silica gel column chromatography,, obtain required product with hexane/ethyl acetate or ethanol/methylene (0%-100%) wash-out.
3. adopt the reductive amination process of amino-quinoline and aldehydes or ketones.
The aldehydes or ketones that the 3-quinolylamine (1 equivalent) that suitably replaces and 1 equivalent is suitable is stirred to formation that TLC shows imines in methyl alcohol (or other The suitable solvent mixture) finish till.Add excessive NaCNBH 4Or NaBH 4, or other suitable reductive agent, and stir the mixture show the completely consumed of intermediate imines to TLC till.Enriched mixture, residue with hexane/ethyl acetate (0-100%) or chloroform/methanol (0-20%) wash-out, obtain required product by silica gel column chromatography.
4.3-the linked reaction of amino substd quinolines and bromophenyl compound
Under the inert atmosphere (as argon atmospher), with 2,2 of the 3-quinolylamine (1 equivalent) that suitably replaces and～1.4 equivalent highly basic (as sodium tert-butoxide), bromophenyl compound that 1 equivalent is suitable, catalytic amount '-two (diphenylphosphino)-1-1 '-dinaphthalene (S-BINAP) and couple (dibenzalacetone)-palladium (Pd (dba) 2) in inert organic solvents (as toluene), mix to stir, and be heated to about 80 ℃ of maintenances and spend the night.Cooling mixture, solubilizing agent (as ether) dilution is filtered, and concentrates and chromatography (with 50%EtOAc/ hexane wash-out), obtains required product.
5. form ether through the Mitsunobu condition by the quinoline that the 3-hydroxyl replaces
With respectively being the THF solution (under about 25 ℃ of about 0-) that 1 normal required alcohol, triphenyl phosphine and diethylazodicarboxylate (DEAD) or suitable equivalent are handled the hydroxy quinoxaline of suitable replacement successively.Reaction process is monitored by TLC, after question response is finished (approximately 1-is about 24 hours), and enriched mixture, residue obtains required product by silica gel column chromatography.
6. the quinoline or the quinoxaline that replace of dealkylation lower alkoxy, alkylation more subsequently
Handle the quinoline of suitable lower alkoxy replacement or the DMF solution of quinoxaline (1 equivalent) with excessive sulfur alcohol sodium (about 2 equivalents or more equivalents usually), under heating, about 24 hours of the about 1-of stirred reaction mixture.Mixture is assigned within water and the ethyl acetate, extracts aftertreatment, carry out chromatography subsequently if necessary, thereby obtain corresponding hydroxyl substd quinolines or the required product of quinoxaline.
Use the Mitsunobu reaction conditions that above describes in detail, quinoline or quinoxaline product that alkylate hydroxyl replaces.Other method is to use method well known in the art, utilizes NaH or other suitable alkali to carry out simple alkylation by active alkylogen or benzyl halide in suitable solvent.
7. the nitrogen in oxidation quinoline or the quinoxaline forms corresponding N-oxide compound
In about room temperature (preferably at elevated temperatures) to reflux temperature, preferably by inert solvent (as methylene dichloride) and peracid (for example peracetic acid or-the chlorine peroxybenzoic acid) reaction, the imines in formula (I) quinoline or the quinoxaline compounds (=N-) partly can be converted into the respective compound that imines wherein partly is oxidized to the N-oxide compound.
The compounds of this invention uses with the form of free alkali or acid or with the form of its pharmacologically acceptable salt, and all these forms all within the scope of the present invention.
When The compounds of this invention is replaced by basic group, can form acid salt, and this is a kind of type of service more simply and easily; And in practice, use salt form to be equivalent to use free alkali form in essence.Those acid that the acid that can be used for preparing acid salt can produce pharmacologically acceptable salt when being preferably included in free alkali chemical combination, that is the negatively charged ion of salt (under the drug dose of salt) is nontoxic to the patient, thereby anionic side effect can not damage the free alkali inherent the useful inhibition of PDGF is renderd a service.Though the pharmacologically acceptable salt of preferred described basic cpd, but, all acid salt all can be used as the source of free alkali, even required concrete salt itself is only also all like this as intermediate (for example as only being used for purifying and identifying purpose and salt that forms or the salt that is used as intermediate in the process for preparing pharmacologically acceptable salt by ion exchange method).Pharmacologically acceptable salt in the scope of the invention is derived from following acid: mineral acid, example hydrochloric acid, sulfuric acid, phosphoric acid and dithiocarbamic acid; And organic acid, as acetate, citric acid, lactic acid, tartrate, propanedioic acid, methylsulfonic acid, ethyl sulfonic acid, Phenylsulfonic acid, right-toluenesulphonic acids, cyclohexyl thionamic acid, quinic acid etc.Corresponding acid salt comprises respectively: halogen acid salt, example hydrochloric acid salt and hydrobromate, vitriol, phosphoric acid salt, nitrate, sulfamate, acetate, Citrate trianion, lactic acid salt, tartrate, malonate, oxalate, salicylate, propionic salt, succinate, fumarate, maleate, methylene radical-bis-beta-hydroxyethyl base naphthoate, gentisate, mesylate, isethionate and two-p-methylphenyl tartrate mesylate, esilate, benzene sulfonate, right-tosylate, cyclohexyl-n-sulfonate and quinate.
According to a further general feature of the present invention, by using or adopt currently known methods, can prepare the acid salt of of the present inventionization thing by free alkali and suitable acid-respons.For example, the acid salt of The compounds of this invention can be prepared as follows: free alkali is dissolved in the water or water-alcohol solution or other suitable solvent that contains suitable acid, and then by the evaporating solns separated salt, free alkali and acid are reacted in organic solvent, in this case, salt can obtain or can obtain by concentrated solution by direct separation.
By using or adopt currently known methods, can be by these acid salt regeneration The compounds of this invention.For example, by with alkali for example sodium bicarbonate aqueous solution or ammonia soln handle, parent compound of the present invention can be obtained by its acid salt regeneration.
When The compounds of this invention is replaced by acidic-group, can form base addition salt, and this is a kind of type of service more simply and easily; And in practice, use salt form to be equivalent to use free acid form in essence.The alkali that can be used for preparing base addition salt can produce those alkali of pharmacologically acceptable salt when being preferably included in free acid chemical combination, that is the positively charged ion of salt (under the drug dose of salt) is nontoxic to animal body, thereby cationic side effect can not damage the useful inhibition effect of free acid inherent to PDGF.Pharmacologically acceptable salt comprises for example basic metal and alkaline earth salt, and these salt within the scope of the present invention are derived from following alkali: sodium hydride, sodium hydroxide, potassium hydroxide, calcium hydroxide, aluminium hydroxide, lithium hydroxide, magnesium hydroxide, zinc hydroxide, ammonia, Trimethylamine 99, triethylamine, quadrol, N-methylglucosamine, Methionin, arginine, ornithine, choline, N, N '-dibenzyl-ethylenediamin, chloroprocaine, diethanolamine, PROCAINE HCL, PHARMA GRADE, N-benzyl-1-phenylethylamine, diethylamine, piperazine, three (hydroxymethyl)-aminomethane, tetramethylammonium hydroxides etc.
The metal-salt of The compounds of this invention can prepare by the The compounds of this invention of free acid form is contacted with hydride, oxyhydroxide, carbonate or the similar activity compound of selected metal.Used water-containing solvent can be water or also can be the mixture of water and organic solvent, organic solvent preferred alcohols wherein such as methyl alcohol or ethanol, and ketone, as acetone, aliphatic ether such as tetrahydrofuran (THF), or ester such as ethyl acetate.These reactions are at room temperature carried out usually, but they carry out under also can heating if desired.
The amine salt of The compounds of this invention can prepare by amine is contacted with the The compounds of this invention of free acid form.Suitable water-containing solvent comprises the mixture of water and water and alcohol (as methyl alcohol or ethanol), ether (as tetrahydrofuran (THF)), nitrile (as acetonitrile) or ketone (as acetone).Equally also can make amino acid salts.
Adopt or adopt currently known methods, can obtain The compounds of this invention by its base addition salt regeneration.For example, by handling with sour example hydrochloric acid, can be from its base addition salt regeneration parent compound of the present invention.
Itself to can be used as active compound the same with them, the salt of The compounds of this invention also can be used for the purifying purpose of compound, for example, use and well known to a person skilled in the art technology, utilize the dissolubility difference between salt and parent compound, by product and/or the initial substance to carry out purifying.
The compounds of this invention may contain asymmetric center.These asymmetric centers can be R or S configuration independently.Those skilled in the art are also fully aware of, and some formula I compound may show geometric isomerism.Geometrical isomer comprises the cis and the trans forms of The compounds of this invention (promptly have and have substituent compound on kiki alkenyl group or the ring system).In addition, bicyclic ring system comprises Nei Shi and external isomer.The present invention includes single geometrical isomer, steric isomer, enantiomer and their mixture.
Adopt or use currently known methods, for example, chromatographic technique and recrystallization technology can be isolated these isomer in its mixture, perhaps also can adopt or method that application examples is as described herein prepares them respectively by the suitable isomer of its intermediate.
Adopt or the application currently known methods, for example the method described in the reference example or its tangible chemistry are equal to method, perhaps use method of the present invention, preparation initial substance and intermediate.
The present invention further is used to illustrate that with following the illustrative embodiment of The compounds of this invention preparation is illustrated, but the present invention is not limited thereto.
Further, the following example is the typical example of synthetic The compounds of this invention method therefor.
Embodiment 13-cyclohexyloxy-6, the 7-dimethoxy-quinoline
Under 0 ℃, in THF solution, add 3-hydroxyl-6, the 7-dimethoxy-quinoline (0.237g, 1.15mmol), hexalin (0.347g, 3.46mmol), Ph 3P (0.908g, 3.46mmol).Add the diethylazodicarboxylate in batches, and till solution keeps scarlet (0.663g, 3.81mmol).After 4 hours, concentrated solution and chromatography residue (50%EtOAc/ hexane).Product isopropanol/hexane recrystallization is the HCl salt form, is white solid (m.p.229-232 ℃ is decomposed).
Embodiment 22-anilino-6-isopropoxy-quinoxaline hydrochloride
Under the argon atmospher, to NaH (0.033g adds 1mL DMF in 0.84mmol), add in batches 2-anilino-6-quinoxaline (0.1g, 0.42mmol)/1.5mL DMF.After 30 minutes, dropwise add the 2-N-PROPYLE BROMIDE, solution is heated to 50 ℃ of reactions 1.5 hours.Add water quenching refrigerative reaction mixture, and be distributed in EtOAc and H 2Within the O, use H 2O (3X), salt water washing, dry (MgSO 4) and concentrate.Chromatography gained residue (30%EtOAc/ hexane) obtains 0.05g dialkyl group product and 0.1g title compound.Press the sample for analysis of described preparation HCl salt: to the Et of free alkali 2Add IPA (Virahol)/HCl in the O/IPA solution, obtain HCl salt (m.p.205-210 ℃ of decomposition).
Ultimate analysis C 17H 17N 3OHCl:
Calculated value: C, 64.65; H, 5.74; N, 13.31;
Measured value: C, 64.51; H, 5.90; N, 13.09.
Embodiment 32-cyclohexyl amino-6,7-dimethoxy quinoxaline
To 0.3g (1.34mmol) 2-chloro-6, add about 1mL hexahydroaniline in the 7-dimethoxy quinoxaline.Mixture is 105 ℃ of following heated overnight, and then 135 ℃ of heating 10 hours.Mixture is distributed in CH 2Cl 2Within saturated sodium bicarbonate.Dry (MgSO 4) organic layer and concentrated.Chromatography gained soup compound (1: 1 EtOAc: CH 2Cl 2), obtaining the 0.265g product, productive rate 69% is bright brown solid (m.p.188-189.5).
Ultimate analysis C 16H 21N 3O 2:
Calculated value: C, 66.88; H, 7.37; N, 14.62;
Measured value: C, 66.82; H, 7.28; N, 14.45.
Utilize above-mentioned standard coupling method, use suitable initial substance to prepare following compounds: outer-two rings [2.2.1] heptan-2-base-(6-chloro-7-methoxyl group quinoxaline-2-yl) amine
(m.p.171-173℃).
Ultimate analysis C 16H 18N 3OCl:
Calculated value: C, 63.26; H, 5.97; N, 13.83;
Measured value: C, 63.37; H, 5.91; N, 13.83.
Outward-two the ring [2.2.1] heptan-2-base-(7-chloro-6-methoxyl group quinoxaline-2-yl) amine
(m.p.146-147.5℃).
Ultimate analysis C 16H 18N 3OCl:
Calculated value: C, 63.26; H, 5.97; N, 13.83;
Measured value: C, 63.34; H, 5.93; N, 13.77.
Two the ring [2.2.1] heptan-2-base-(6,7-dimethyl quinoxaline-2-yl) amine
(m.p.155-57℃).
Ultimate analysis C 17H 21N 3:
Calculated value: C, 76.37; H, 7.92; N, 15.72;
Measured value: C, 75.58; H, 7.55; N, 15.38.
2-suberyl amino-6,7-dimethoxy quinoxaline
(m.p.134-136℃).
Ultimate analysis C 17H 23N 3O 2:
Calculated value: C, 67.75; H, 7.69; N, 13.94;
Measured value: C, 67.80; H, 7.61; N, 13.77.
2-cyclopentyl amino-6,7-dimethoxy quinoxaline
(m.p.149-151℃).
Ultimate analysis C 15H 19N 3O 2:
Calculated value: C, 65.91; H, 7.01; N, 15.37;
Measured value: C, 66.04; H, 6.96; N, 15.47.
2-cyclohexyl amino-6-methoxyl group quinoxaline
(m.p.242-248℃).
Embodiment 43-aminocyclohexyl-6,7-dimethoxy yl-quinoline
Under the argon atmospher, to Add 3-amino-6 in methyl alcohol (3mL) solution of mealy molecular sieve (0.11g), 7-dimethoxy-quinoline hydrochloride (0.17g, 0.68mol) and NaOMe (0.039g, 0.71mmol).Stirred reaction mixture is 30 minutes under the room temperature, add successively then in batches hexanaphthene ketone (0.074mL, 0.71mmol) and the pyridine borine (0.072mL, 0.071mmol).Stirred the mixture 4.5 hours, add then in batches 5N HCl (1.4mL, 6.8mmol).Stirred reaction mixture 45 minutes is adjusted to strong basicity with 5N NaOH then.Mixture is distributed in EtOAc and H 2Within the O, water layer is washed with EtOAc (2X).Merge organic layer, with salt solution (1X) washing, dry (MgSO 4), chromatography (50%EtOAc/ hexane) with EtOAc/ hexane recrystallization, obtains the 0.112g light yellow solid, productive rate 57% (m.p.164-165).
Ultimate analysis C 17H 22N 2O 2:
Calculated value: C, 71.30; H, 7.74; N, 9.78;
Measured value: C, 71.45; H, 7.49; N, 9.80.
Embodiment 52-cyclohexyl amino-6-methoxyl group-7-bromo-quinoxaline hydrochloride
0.75g in placing sealed tube (2.7mmol) 7:17-bromo-6-methoxyl group-quinoxaline-2-alcohol: 6-bromo-7-methoxyl group-quinoxaline-2-alcohol adds the 5mL hexahydroaniline.Reacting by heating mixture to 120 ℃ reaction 2 hours.Hexahydroaniline is removed in decompression, and residue is distributed in EtOAc/H 2In the O.Organic layer water (2X), salt solution (1X) washing, and dry (MgSO 4).Chromatography gained material (using 20%EtOAc/ hexane and 30%EtOAc/ hexane wash-out successively) obtains the 0.81g principal product, productive rate 88%.By about 0.13g free alkali being converted into its hydrochloride (m.p.280 ℃ is decomposed), obtain analytic sample.
Ultimate analysis C 15H 18N 3OBrHCl:
Calculated value: C, 48.34; H, 5.14; N, 11.27;
Measured value: C, 48.51; H, 4.98; N, 11.09.
Embodiment 6(6,7-dimethoxy-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base)
Amine dihydrochloride and (6,7-dimethoxy yl-quinoline-3-yl)-trans-(3-(R)-methyl-cyclohexyl base) amine dihydrochloride
Separate reduction amination 3-amino-6 by RP-HPLC, suitable/anti-(6,7-dimethoxy yl-quinoline-3-yl)-(3-(R)-methyl-cyclohexyl base)-amine mixt that 7-dimethoxy-quinoline and 3-(R)-methylcyclohexanone is made.These two kinds of samples of chromatography (70%EtOAc/ hexane) obtain pure free alkali again.By respectively the free alkali of these two kinds of isomer being converted into the amorphous and the dihydrochloride of moisture absorption to a certain extent, prepare their analytic samples separately.With regard to each isomer, 500MHz 1H NMR is consistent with product, and LC/MS and FAB confirm M+H=301.
Embodiment 7Hexamethylene-3-thiazolinyl-(6,7-dimethoxy quinoxaline-2-yl)-amine
Under-78 ℃, to trans-4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexalin (add in the 303mg, 10mL THF solution 1mmol) triphenyl phosphine (524mg, 2mmol) and diethylazodicarboxylate (1mL).Mixture was stirred 1 hour at-78 ℃, add then the 4-nitrobenzoic acid (334mg, 2mmol).-78 ℃ further stir 1 hour after, mixture continued at room temperature restir 1 hour, concentrated then.Silica gel column chromatography residue (ether) obtains 250mg (87.7%) hexamethylene-3-thiazolinyl-(6,7-dimethoxy quinoxaline-2-yl)-amine.
Embodiment 82-anilino-6-quinoxaline
Adopt Feutrill, G.I.; Mirrington, R.N. exists The tetrahedron communication(Tet.Lett.), described in 1970,1327 aryl methyl ether is converted into amphyl method.Under the argon atmospher, to 2-anilino-6-methoxyl group-quinoxaline (0.27g, add in DMF solution 1.07mmol) the sulfur alcohol sodium salt (0.19g, 2mmol).Reacting by heating mixture to 110 ℃ spends the night.Enriched mixture, and be assigned to EtOAc and H 2Within the O/5% tartrate, make the pH of water layer approximate 4 greatly.Organic layer is water (4X) and 2.5%NaOH (4X) washing successively.Merge alkaline layer, with ethyl acetate (2X) washing, use 5% tartrate acidifying again, and wash with many parts of EtOAc.Merge organic layer, use the salt water washing, dry (Na 2SO 4), and concentrate.Chromatography gained solid (50%EtOAc/ hexane).By using Et 2O develops product, obtains yellow powder analytic sample (m.p.211-213 ℃).
Ultimate analysis C 14H 11N 3O:
Calculated value: C, 70.88; H, 4.67; N, 17.71;
Measured value: C, 70.64; H, 4.85; N, 17.58.
Embodiment 9Phenyl-[6-(tetrahydrofuran (THF)-3-(R)-Ji-oxygen base) quinoxaline-2-yl] amine
Under 0 ℃ and the argon atmospher, in THF solution, add 2-anilino-6-quinoxaline (0.23g, 0.97mmol), (S)-(+)-the 3-hydroxyl tetrahydrofuran (0.086mL, 1.3mmol), and triphenyl phosphine (0.31g, 1.2mmol).Add in batches DEAD (0.18mL, 1.2mmol).The warm reaction to room temperature stirred 1.5 hours.Enriched mixture also is assigned to it within EtOAc and the water.Organic layer water and salt water washing, dry (MgSO 4) and concentrate.Chromatography gained yellow oil (50%EtOAc/ hexane), and be dissolved in Et 2O/IPA.Dropwise add HCl/Et 2O solution, vacuum-drying gained orange powder.By containing washed (3XH 2O 5XMeOH) stirs among the MeOH of deacidite, with the alkalization that dissociates of gained powder.Stirred the mixture 30 minutes, and filtered, concentrate, and, obtain product (m.p.173-175 ℃) in two batches with EtOAc/ hexane recrystallization.
Ultimate analysis C 18H 17N 3O 2:
Calculated value: C, 70.35; H, 5.57; N, 13.67;
Measured value: C, 70.19; H, 5.60; N, 13.66.
Embodiment 102,7-pair-cyclohexyloxy-6-methoxyl group-quinoxaline
Under the argon atmospher, to NaH (0.32g, dropwise add in DMF solution 8mmol) hexalin (0.7mL, 6.7mmol).Stirring at room mixture 25 minutes adds 2-chloro-6 then in batches, 7-dimethoxy quinoxaline.Stirring at room reaction 15 minutes, and then stirred 2 hours and stirred 1 hour at 110 ℃ at 90 ℃ respectively.Cooling mixture adds the water quenching, and is assigned to EtOAc/H 2Within the O.Organic layer water and salt water washing, dry (MgSO 4), obtain wax shape white solid thing (m.p.75-78 ℃) behind the chromatography (10%EtOAc/ hexane).
Ultimate analysis C 21H 28N 2O 3:
Calculated value: C, 70.76; H, 7.92; N, 7.86;
Measured value: C, 70.81; H, 7.79; N, 7.70.
Embodiment 11Cyclohexyl-(6,7-dimethoxy quinoxaline-2-ylmethyl)-amine
To 6,7-dimethoxy-2-quinoxaline formaldehyde is at 2:1MeOH/1, the 2-ethylene dichloride (7.5mL, 0.5mmol) add in the 0.067M solution in hexahydroaniline (0.11mL, 0.9mmol).At room temperature stirring reaction spends the night, and adds NaBH then 4(0.038g, 1mmol), the restir reaction mixture spends the night.Enriched mixture and carry out chromatography (50%EtOAc/ hexane-approximately contain the 50%EtOAc/ hexane of 5%MeOH) then.Gained oily matter is dissolved in the EtOAc/ hexane, uses HCl/Et 2O handles.Concentrate gained solution, solids is developed with Virahol, obtains white solid (m.p.185-190 ℃ is decomposed) after 60 ℃ of vacuum-drying.
Ultimate analysis C 17H 23N 3O 2HCl:
Calculated value: C, 60.44; H, 7.16; N, 12.44;
Measured value: C, 60.48; H, 6.88; N, 12.07.
Embodiment 12(6,7-dimethoxy-quinoline-3-yl)-trans-(3-(R)-methyl-cyclohexyl
Base) amine and (6,7-dimethoxy-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base) amine
Preparation example above similar adopts 3-amino-6, and 7-dimethoxy-quinoline free alkali (0.32g, 1.6mmol) and (R)-(+)-(0.23mL 1.9mmol) reacts the 3-methylcyclohexanone.Chromatography products therefrom mixture (70%EtOAc/ hexane), and with EtOAc/ hexane recrystallization, thereby white solid thing (1: 1 cis-trans isomer mixture) (m.p.153-160 ℃) obtained.
Ultimate analysis C 18H 24N 2O 2:
Calculated value: C, 71.97; H, 8.05; N, 9.33;
Measured value: C, 72.12; H, 7.85; N, 9.29.
Adopt above-mentioned standard coupling method, use suitable initial substance and prepare following compound: (6,7-dimethoxy-quinoline-3-yl)-(3-methyl-cyclopentyl) amine
(m.p.106-109℃)。
Ultimate analysis C 17H 22N 2O 2:
Calculated value: C, 71.30; H, 7.74; N, 9.78;
Measured value: C, 71.24; H, 7.56; N, 9.61.
Embodiment 133-(6,7-dimethoxy-quinoline-3-base-amino)-2,2-dimethyl-third-1-alcohol
Preparation process in the similar embodiment 11 is reacted.Under the argon atmospher, to
Figure A20081010053600431
Add 3-amino-6 in the MeOH solution of mealy molecular sieve (0.35g), and the 7-dimethoxy-quinoline (0.32g, 1.6mmol) with 2, the 2-pentaldol (0.19g, 1.9mmol).Chromatography product mixtures (3%MeOH/CHCl 3), obtain the 0.10g material, and then be assigned to CH 2Cl 2Within/the 10%NaOH.Organic layer 10%NaOH, H 2O and salt water washing, dry then (MgSO 4), with EtOAc/ hexane recrystallization, obtain light orange solids (m.p.170-173.5 ℃).
Ultimate analysis C 16H 22N 2O 3:
Calculated value: C, 66.18; H, 7.64; N, 9.65;
Measured value: C, 66.11; H, 7.49; N, 9.33.
Adopt above-mentioned standard coupling method, use suitable initial substance to prepare following compounds: (6,7-dimethoxy-quinoline-3-yl)-isobutylamine
(m.p.158-162℃).
Ultimate analysis C 15H 20N 2O 2:
Calculated value: C, 69.20; H, 7.74; N, 10.76;
Measured value: C, 69.06; H, 7.82; N, 11.01.
Embodiment 14Cyclohexyl-(6-methoxyl group-7-morpholine-4-base-quinoxaline-2-yl)-amine
This preparation is based on the method for the Buchwald that revises etc. (American Chemical Society can will, 1996,118,7215).Under the argon atmospher, to 2-cyclohexyl amino-6-methoxyl group-7-bromo-quinoxaline (0.1g, 0.3mmol) toluene solution in add morpholine (0.1g, 0.3mol), sodium tert-butoxide (0.04g, 0.42mmol), S-(-)-BINAP (about 0.001g) and two (dibenzalacetones) close palladium (about 0.001g).Reacting by heating mixture to 80 ℃ spends the night.Cooling mixture, the dilution that adds diethyl ether is filtered, concentrates, and chromatography (50%EtOAc/ hexane).Product obtains yellow solid (m.p.194-196 ℃) in two batches with EtOAc/ hexane recrystallization.
Ultimate analysis C 19H 26N 4O 2:
Calculated value: C, 66.64; H, 7.65; N, 16.36;
Measured value: C, 66.60; H, 7.60; N, 16.51.
Embodiment 15Trans-4-(7-chloro-6-methoxyl group-quinoxaline-2-amino)-hexalin and trans-4-(6-chloro-7-methoxyl group-quinoxaline-2-base-amino)-hexalin
Under the argon atmospher, in the reaction flask of being furnished with Dean Stark water trap and condenser, add 6: 12,7-two chloro-6-methoxyl group-quinoxalines: 2,6-two chloro-7-methoxyl groups-quinoxaline mixture (0.30g, 1.3mmol) and trans-4-amino-hexalin (0.35g, 3mmol).About 10 hours of reacting by heating mixture to 170 ℃ reaction concentrates and twice (7%MeOH/CHCl of chromatography then 3, 5%MeOH/CHCl then 3).Product ethyl acetate/hexane recrystallization obtains light yellow solid thing (m.p.144-147 ℃).
Ultimate analysis C 19H 26N 4O 20.4H 2O:
Calculated value: C, 57.20; H, 6.02; N, 13.34;
Measured value: C, 57.21; H, 5.97; N, 13.08.
1H NMR analysis revealed, product are 2: 1 mixtures of trans-4-(7-chloro-6-methoxyl group-quinoxaline-2-amino)-hexalin and trans-4-(6-chloro-7-methoxyl group-quinoxaline-2-base-amino)-hexalin.
Embodiment 16Trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexalin
Mix trans-4-Trans-4-Amino Cyclohexanol (0.11g, 2 equivalents) and 2-chloro-6,7-dimethoxy quinoxaline (0.1g, 1 equivalent), and be heated to 160-180 ℃ of reaction 4-8 hour.Filter the dark brown suspension of gained and concentrated.Residue with 3% ethanol/methylene wash-out, obtains the yellow powder product by quick column purification, and m.p. is 119-123 ℃.
Ultimate analysis C 16H 21N 3O 3:
Calculated value: C, 62.33; H, 7.05; N, 13.63;
Measured value: C, 62.35; H, 7.09; N, 13.18.
The gained compound is recrystallization as follows.The 0.2g yellow powder is joined in 2.5mL water and the 1.25mL methyl alcohol, and refluxing obtains orange clear solution.Place this hot solution, progressively cooling.Filter and collect the orange crystallization of needle-like, obtain yellow solid (m.p.119-120 ℃) after the high vacuum dry.
On the other hand, press the HCl salt of described preparation title compound: under 0 ℃, in the aqueous isopropanol of trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexalin, add HCl solution.Stirred the mixture 15 minutes, and filtered then.Dry collected solids under high vacuum obtains trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexalin hydrochloride.
Ultimate analysis C 16H 22ClN 3O 31.2H 2O:
Calculated value: C, 53.19; H, 6.80; N, 11.63; Cl 9.81;
Measured value: C, 53.14; H, 6.85; N, 11.24; CL, 10.28.
On the other hand, the vitriol of title compound can be pressed described preparation: in typical method, trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexalin is dissolved in acetone or other The suitable solvent, is heated to 45 ℃ if desired.Under stirring fast, the careful aqueous sulfuric acid (1 equivalent, 1M solution) that adds in gained solution.Collect the salt that so forms, obtain vitriol after the drying, productive rate＞80%.
Embodiment 17(±)-two ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine
Method A: with 2-chloro-6, (5g is 22.3mmol) with (±)-outward-(10g, mixture 90mmol) is 160-180 ℃ of following heated overnight for norcamphyl-2-amine for 7-dimethoxy quinoxaline.The dark-brown residue is dissolved in the 200mL methylene dichloride, with 1N NaOH (50mL) washing.With the dried over mgso organic layer, filter then.Residue after silica gel column chromatography concentrates with hexane/ethyl acetate (80%) wash-out, obtains required product, is yellow solid, can use recrystallizing methanol.
Method B: with 2-chloro-6,7-dimethoxy quinoxaline (9g, 40.1mmol) and (±)-outer-norcamphyl-2-amine (5.77g, 52mmol), sodium tert-butoxide (4.22g, 44mmol), 2,2 '-(BINAP is 120mg) with two (dibenzalacetone)-palladium Pd (dba) for two (diphenylphosphino)-1-1 '-dinaphthalene 2Mixture in 80mL toluene was 80 ℃ of heating 8 hours.Add another part BINAP (60mg) and Pd (dba) again 2(20mg), spend the night at 100 ℃ of heated mixt.After adding the dilution of 200mL methylene dichloride, reaction mixture is washed with 1N NaOH (100mL).Use the dried over mgso organic layer, and filter.Residue after silica gel column chromatography concentrates with hexane/ethyl acetate (80%) wash-out, obtains required product, is light yellow solid thing (m.p.188-189 ℃).
Ultimate analysis C 17H 21N 3O 3:
Calculated value: C, 68.20; H, 7.07; N, 14.04;
Measured value: C, 68.18; H, 7.03; N, 14.03.
Similarly, begin to make following compounds (method A): outer-two rings [2.2.1] heptan-5-alkene-2-base-(6,7-dimethoxy quinoxaline-2-yl) amine by suitable initial substance
(m.p.175-177℃)。
Ultimate analysis C 17H 19N 3O 2:
Calculated value: C, 60.94; H, 6.56; N, 13.78;
Measured value: C, 66.98; H, 6.62; N, 12.73.
Cyclohexyl-(6,8-dimethyl-quinoxaline-2-yl)-amine [MS m/z:255 (M+)].
Ultimate analysis C 16H 21N 3:
Calculated value: C, 75.26; H, 8.29; N, 16.46;
Measured value: C, 75.08; H, 8.28; N, 15.86.
In-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl) amine (m.p.79-82 ℃).
(6,7-dimethoxy quinoxaline-2-yl)-(4-methoxyl group-cyclohexyl)-amine (m.p.58-68 ℃).
Ultimate analysis C 17H 23N 3O 30.5H 2O:
Calculated value: C, 62.56; H, 7.41; N, 12.87;
Measured value: C, 62.53; H, 7.22; N, 12.22.
Outward-two the ring [2.2.1] heptan-2-base-(6-methoxyl group quinoxaline-2-yl)-amine (m.p.98-100 ℃).
Ultimate analysis C 16H 19N 3O:
Calculated value: C, 71.35; H, 7.11; N, 15.60;
Measured value: C, 70.38; H, 7.03; N, 15.05.
Embodiment 18Outward-and 2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy quinoxaline
Will outer-2-norborneol (223mg, 2mmol) and NaH (60%, 100mg, 2.5mmol) backflow of the mixture in the anhydrous THF of 10mL is 0.5 hour, adds 2-chloro-6 then, 7-dimethoxy quinoxaline (336mg, 1.5mmol).Continued backflow gained mixture 2 hours.Filtering the back and concentrate, and then by silica gel column chromatography gained residue (50% ether/hexane), obtain required product, is white solid (m.p.135-137 ℃).
Ultimate analysis C 17H 20N 2O 3:
Calculated value: C, 67.98; H, 6.71; N, 9.33;
Measured value: C, 67.96; H, 6.762; N, 9.19.
Employing standard coupling method prepares following compounds by suitable initial substance:
2-(two ring [2.2.1] sufferings-2-base oxygen base)-6,7-dimethoxy-quinoxaline (m.p.147-148 ℃).
In-2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy quinoxaline (m.p.110-111 ℃).
Outward-and 2-(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline (m.p.108-110 ℃).
Ultimate analysis C 17H 18N 2O 3:
Calculated value: C, 68.44; H, 6.08; N, 9.39;
Measured value: C, 68.54; H, 6.23; N, 9.27.
2-(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline (m.p.93-95 ℃).
Ultimate analysis C 17H 18N 2O 3:
Calculated value: C, 68.44; H, 6.08; N, 9.39;
Measured value: C, 68.32; H, 5.98; N, 9.25.
2-cyclohexyloxy-6,7-dimethoxy quinoxaline (m.p.104-106 ℃).
2-encircles penta sulfenyl-6,7-dimethoxy-quinoxaline (m.p.123-124 ℃).
Ultimate analysis C 15H 18N 2O 2S:
Calculated value: C, 62.04; H, 6.25; N, 9.65;
Measured value: C, 61.90; H, 6.02; N, 9.48.
6,7-dimethoxy-2-cyclopentyloxy-quinoxaline (m.p.87-89 ℃).
Ultimate analysis C 15H 18N 2O 3:
Calculated value: C, 65.68; H, 6.61; N, 10.21;
Measured value: C, 65.63; H, 6.52; N, 10.13.
2-cyclopentyl methoxyl group-6,7-dimethoxy-quinoxaline (m.p.99-102 ℃).
Ultimate analysis C 16H 20N 2O 3:
Calculated value: C, 66.65; H, 6.99; N, 9.72;
Measured value: C, 66.66; H, 7.03; N, 9.70.
6,7-dimethoxy-2-tetrahydropyrans-4-oxygen base-quinoxaline (m.p.155-158 ℃).
Ultimate analysis C 15H 18N 2O 4:
Calculated value: C, 62.06; H, 6.25; N, 9.65;
Measured value: C, 62.26; H, 6.27; N, 9.67.
Outward, outer-6,7-dimethoxy-2-(5,6-epoxy-two ring [2.2.1] heptane-2-base oxygen base)-quinoxaline (m.p.173-175 ℃).
Embodiment 19Suitable/anti--4-(6,7-dimethoxy quinoxaline-2-base oxygen base)-hexahydrobenzoic acid
With suitable/anti--4-hydroxyl-hexahydrobenzoic acid (144mg, 1mmol) and NaH (60%, 160mg, 4mmol) backflow of the mixture heating up in anhydrous THF/DMF (10mL/2mL) is 1 hour, adds 2-chloro-6 then, 7-dimethoxy quinoxaline (225mg, 1mmol).Continued backflow gained mixture 4 hours.The neutralization reaction mixture extracts with ethyl acetate (2x50mL) to pH5.Also filter with the organic solution that dried over mgso merges.Residue after silica gel column chromatography concentrates (use ethyl acetate earlier, then use methanol-eluted fractions) obtains required product, is white solid thing (m.p.90-93 ℃).
Ultimate analysis C 17H 20N 2O 50.5H 2O:
Calculated value: C, 59.89; H, 6.19; N, 8.22;
Measured value: C, 59.91; H, 6.62; N, 7.90.
Embodiment 206,7-dimethoxy-2-(4-methoxyl group-cyclohexyloxy)-quinoxaline
Under 0 ℃, stir suitable/anti--4-(6,7-dimethoxy-quinoxaline-2-base oxygen base)-hexalin (170mg, 0.56 mmol) and NaH (60%, 22.4mg, 0.56mmol) mixture in anhydrous THF/DMF (10mL/2mL) is 10 minutes, add then methyl-iodide (50 μ L, 0.56mmol).Stir after 4 hours under the room temperature, add water (0.5mL) termination reaction, concentrate.Water layer extracts with methylene dichloride (2x20mL), and the organic solution that merges is washed with salt solution (5mL).Residue (30% ethyl acetate/hexane) after silica gel column chromatography concentrates obtains the required product of 80mg (45%) (m.p.85-90 ℃).
Embodiment 213-cyclohexyloxy-6,7-dimethoxy quinoxaline 1-oxide compound
With 2-cyclohexyloxy-6,7-dimethoxy quinoxaline (10mg, 0.38mmol) and metachloroperbenzoic acid (70%, 113mg, 0.46mmol) the mixture stirring at room in the 10mL methylene dichloride is 1 day.Filter the back concentrated solution, residue obtains required product (m.p.167-169 ℃) by silica gel column chromatography (20% ethyl acetate/hexane).
Can make trans-4-(6,7-dimethoxy-4 '-oxygen base-quinoxaline-2-base is amino)-hexalin (m.p.220-222 ℃) similarly.
Ultimate analysis C 16H 21N 3O 40.2H 2O:
Calculated value: C, 59.42; H, 6.69; N, 12.99;
Measured value: C, 59.43; H, 6.64; N, 12.95.
Embodiment 22(1R, 2R, 4S)-(+)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine
Utilize chirality HPLC post (Chiralpac AD, 25x2cm, 60% heptane/40% ethanol that contains 10mM (1S)-(+)-camphorsulfonic acid, 12mL/ minute) split (±)-two rings [2.2.1] heptan-2-base-(6 of embodiment 17,7-dimethoxy quinoxaline-2-yl)-and amine, above-mentioned title compound obtains with the first eluate form.Merge collected cut, with the NaOH washing of 50ml 1N, dry then (MgSO 4).Solution after the filtration concentrates with rotatory evaporator, and is dry under high vacuum then.Obtain yellow solid.[α] d 20+ 19.5 ° of (c=0.20, CH 2Cl 2), m.p.184-186 ℃. ultimate analysis C 17H 21N 3O 2X0.3H 2O:
Calculated value: C, 66.90; H, 7.15; N, 13.77;
Measured value: C, 66.86; H, 7.01; N, 13.86.
Embodiment 23(1S, 2S, 4R)-(-)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine
(i) utilize chirality HPLC post (Chiralpac AD, 25x2cm contains 60% heptane/40% ethanol of 10mM (1S)-(+)-camphorsulfonic acid, 12mL/ minute) split (±)-two rings [2.2.1] heptan-2-base-(6 of embodiment 17,7-dimethoxy quinoxaline-2-yl)-and amine, as second eluate.Merge collected cut, with 50mL 1N NaOH washing, then with dried over mgso.Utilize the solution behind the rotatory evaporator thickening filtration, dry under high vacuum then.Obtain yellow solid.[α] d 20-19.5°(c＝0.22，CH 2Cl 2)，m.p.185-187℃.
(ii) with 2-chloro-6,7-dimethoxy quinoxaline (462mg, 2.06mmol) and (1S, 2S, 4R)-norcamphyl-2-amine (300mg, 2.7mmol), sodium tert-butoxide (220mg, 2.3mmol), BINAP (9mg) and Pd (dba) 2(3mg) mixture in 10mL toluene is 80-100 ℃ of following heated overnight.Suspension with hexane/ethyl acetate (60%) wash-out, obtains the required product of 370mg (60%) by silica gel column chromatography, is yellow solid, and this solid has identical retention time with first eluate under above-mentioned chirality HPLC condition.[α] d 20-19°(c＝0.19，CH 2Cl 2).
Embodiment 242-(6,7-dimethoxy-quinoxaline-2-yl)-2-aza-bicyclo [2.2.2] octane-3-ketone
(228mg 2.3mmol) is dissolved in THF/DMF (5mL/3mL) mixed solution, and (60%, 184mg 4.6mmol) handles with NaH with 2-azabicyclic [2.2.2] octane-3-ketone.Heat the gained mixture 0.5 hour down at 60 ℃, add 2-chloro-6 then, and 7-dimethoxy quinoxaline (344mg, 1.5mmol).After 80 ℃ of heated overnight, concentrated reaction mixture.Residue obtains 164mg (23%) yellow solid (m.p.158-159 ℃) by silica gel column chromatography (50% ethyl acetate/hexane).
Embodiment 25Suitable/anti--4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters
To 2-(6,7-dimethoxy-quinoxaline-2-yl)-and 2-aza-bicyclo [2.2.2] octane-3-ketone (the freshly prepd NaOMe/ methanol solution of adding in the 100mg, 10mL methanol solution 0.32mmol) (54mg, 1mmol), stirring at room mixture 0.5 hour concentrates then.Use dichloromethane extraction, use dried over mgso subsequently.Residue behind the thickening filtration, and silica gel column chromatography (40% ethyl acetate), obtain 85mg (77%) suitable/anti--4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters, be light yellow solid (m.p.68-80 ℃).
Embodiment 26Suitable/anti--4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexahydrobenzoic acid
When the NaOMe that replaces with NaOH in the aforesaid method, 2-(6,7-dimethoxy quinoxaline-2-yl)-2-aza-bicyclo [2.2.2] octane-3-ketone just is converted into suitable/anti--4-(6,7-dimethoxy-quinoxaline-2-base is amino) hexahydrobenzoic acid.
Embodiment 27Cis-4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters and trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters
Use 65% ethyl acetate/hexane wash-out, by preparing TLC by suitable/anti--4-(6,7-dimethoxy-quinoxaline-2-base is amino)-the hexahydrobenzoic acid methyl esters is separated to cis-4-(6,7-dimethoxy-quinoxaline-2-base the is amino)-hexahydrobenzoic acid methyl esters [MS m/z:345 (M that is respectively first and second eluates +)] and trans-4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters [MS m/z:345 (M +)].
Embodiment 28Trans-4-[7-methoxyl group-6-(2-morpholine-4-base-oxyethyl group)-quinoxaline-2-base is amino]-hexalin and trans-4-[6-methoxyl group-7-(2-morpholine-4-base-oxyethyl group)-quinoxaline-2-base amino]-hexalin
Title compound is prepared as follows: the method for utilizing embodiment 1, make 6-hydroxyl-7-methoxyl group-2-chloro-quinoxaline: 7-(2-morpholine-4-base oxethyl)-6-methoxyl group-2-chloro-quinoxaline and 2-(morpholine-4-yl) ethanol carry out the Mitsunobu coupling, adopt the method for embodiment 11 subsequently, make gained 6-(2-morpholine-4-base oxethyl)-7-methoxyl group-2-chloro-quinoxaline: 7-(2-morpholine-4-base oxethyl)-6-methoxyl group-2-chloro-quinoxaline and trans-4-amino-hexalin reaction.
Embodiment 292-[2-(trans-4-hydroxyl-cyclohexyl amino)-7-methoxyl group-quinoxalin-6-yl oxygen base]-1-acetate and 2-[2-(trans-4-hydroxyl-cyclohexyl amino)-6-methoxyl group-quinoxaline-7-base oxygen base]-1-acetate
Title compound is prepared as follows: as described in embodiment 8, utilize sulfur alcohol sodium salt dealkylation 4-(6,7-dimethoxy quinoxaline-2-base is amino) hexalin in DMF, carry out alkylation with bromoacetic acid by general method 6 is described in the presence of alkali subsequently.
Embodiment 302-[2-(trans-4-hydroxyl-cyclohexyl amino)-7-methoxyl group-quinoxalin-6-yl oxygen base]-N, N-dimethyl-ethanamide and 2-[2-(trans-4-hydroxyl-cyclohexyl amino)-6-methoxyl group-quinoxaline-7-base oxygen base]-N, N-dimethyl-ethanamide
Adopt dimethylamine ammonia to separate the compound of embodiment 29, make title compound.
Embodiment 31(6,7-dimethoxy-quinoxaline-2-yl)-(3-(R)-methylcyclohexyl) amine and cis-trans-isomer thereof
The cis-trans-isomer mixture that at first prepares described compound.By the reduction hexahydroaniline that 3-(R)-methylcyclohexanone oxime obtains, then the application standard condition makes described amine and 2-chloro-6, the coupling of 7-dimethoxy quinoxaline, thus make them.The analytic sample of each isomer obtains by preparation property RP-HPLC.300MHz 1H NMR is consistent with these two kinds of structures with MS, but can not clearly point out the relative stereochemistry of the cyclohexyl-carbon that has nitrogen.
Embodiment 32Suitable/anti--4-(6,7-dimethoxy quinoxaline-2-base oxygen base)-hexahydrobenzoic acid methyl esters
Utilize the product of standard method esterification embodiment 19, thereby make title compound.M.p.130-132℃。
Ultimate analysis C 18H 22N 2O 5:
Calculated value: C, 62.42; H, 6.40; N, 8.09;
Measured value: C, 62.60; H, 6.55; N, 7.89.
Intermediate embodiment 14-bromo-5-methoxyl group-benzene-1,2-diamines dihydrochloride
Under the argon atmospher, (add 5%Pd/C (0.5g) in the 2.5g, solution 10mmol) to EtOAc (50mL) and 5-bromo-4-methoxyl group-2-nitro-aniline.Hydrogenation mixture is 1 hour under 50psi.By diatomite, mixture is filled in the HCl/IPA/EtOAc solution, filter bed washs with other EtOAc.Leach the gained throw out, obtain white solid.
Intermediate embodiment 2The 7-bromo-6-methoxyl group-quinoxaline-pure and mild 6-bromo-of 2-7-methoxyl group quinoxaline-2-alcohol
Under the argon atmospher, and adding atomizing NaOH flap in MeOH solution (15mL) (0.86g, 21mmol) with 4-bromo-5-methoxyl group-benzene-1,2-diamines dihydrochloride (2.7g, 9.3mmol).Stirred the mixture 10 minutes, add then in batches 45% glyoxylic acid ethyl ester toluene solution (2.7g, 12mmol).Reaction mixture refluxed 1 hour, cooling then.Add entry, subsequent filtration suspension.The gained solid is water, methyl alcohol, IPA and ether washing in succession, obtains yellow powder.
Intermediate embodiment 37-bromo-2-chloro-6-methoxyl group-quinoxaline and 6-bromo-2-chloro-7-methoxyl group-quinoxaline
Mixture (1g, 3.9mmol) the middle POCl that adds to the 7-bromo-6-methoxyl group-quinoxaline-pure and mild 6-bromo-of 2-7-methoxyl group-quinoxaline-2-alcohol 3(5mL).Reaction mixture refluxed 1 hour is poured in the frozen water, filters, and washing then, thus obtain light brown solid.7-bromo-2-chloro-6-methoxyl group quinoxaline: the ratio of 6-bromo-2-chloro-7-methoxyl group-quinoxaline be approximately 7: 1 (by 1H NMR determines).
Intermediate embodiment 45-chloro-4-methoxyl group-2-N-methyl-p-nitroaniline
(2g 8.2mmol) adds 1 in the solution of 5N HCl (20mL), 4-diox (10mL) stirs mixture 1.5 hours at 60 ℃ to N-(5-chloro-4-methoxyl group-2-nitrophenyl)-ethanamide.Concentrated reaction mixture also is assigned within the EtOAc/2N NaOH.Water layer EtOAc (3X), salt water washing, dry (MgSO 4), be adsorbed on thereafter and carry out chromatography (70%EtOAc/ hexane) on the silica gel, obtain orange powder.
Middle embodiment 54-chloro-5-methoxyl group-benzene-1,2-diamines dihydrochloride
Under the argon atmospher, (add 5%Pd/C (0.5g) in the 1.6g, solution 7.9mmol) to EtOAc (25mL) and 5-chloro-4-methoxyl group-2-nitro-aniline.Hydrogenation mixture is 1 hour under 50psi.Under nitrogen atmosphere, mixture is filled into 1N HCl/Et by diatomite 2In the EtOAc solution of O, filter bed washs with other EtOAc.Leach the gained throw out, obtain the white solid thing.
Intermediate embodiment 6The 7-chloro-6-methoxyl group-quinoxaline-pure and mild 6-chloro-of 2-7-methoxyl group-quinoxaline-2-alcohol
Under 0 ℃ and the argon atmospher, to 4-chloro-5-methoxyl group-benzene-1,2-diamines dihydrochloride (add in the 1.8g, EtOH 7.2mmol) (15mL) solution TEA (2.5mL, 18mmol).Stirred the mixture 20 minutes, add then in batches 45% glyoxylic acid ethyl ester toluene solution (2.1g, 9.3mmol).Warm reaction mixture refluxed 1.5 hours to room temperature, then cooling.Add water, subsequent filtration suspension, and water, IPA and ether washing in succession obtain buff powder.Before the use, earlier with product and methylbenzene azeotropic several, vacuum-drying thereafter.
Intermediate embodiment 72,7-two chloro-6-methoxyl group-quinoxalines and 2,6-two chloro-7-methoxyl group-quinoxalines
CaCl is being arranged 2Drying tube exists down, to mixture (1g, 4.7mmol) the middle POCl that adds of the 7-chloro-6-methoxyl group-quinoxaline-pure and mild 6-chloro-of 2-7-methoxyl group-quinoxaline-2-alcohol 3(5mL).Reaction mixture refluxed 30 minutes is poured in the saturated sodium bicarbonate cold soln, filters, and washing obtains a solids then.Wherein 2,7-two chloro-6-methoxyl group-quinoxalines and 2, the ratio of 6-two chloro-7-methoxyl group-quinoxalines be approximately 6: 1 (by 1H NMR determines).
Intermediate embodiment 8(1S, 2S, 4R)-norcamphyl-2-amine
(3a): under-78 ℃, to R-(+)-Nei-norborneol (2.24g, add in 20mL THF solution 20mmol) triphenyl phosphine (6.55g, 25mmol), phthalic imidine (3.68g, 25mmol) and the diethylazodicarboxylate (4.4mL, 28mmol).Mixture at room temperature stirs and spends the night, and concentrates thereafter.Residue is by silica gel column chromatography (20% ethyl acetate/hexane), obtain 4.6g (95%) (1S, 2S, 4R)-2-two rings [2.2.1] heptan-2-base isoindole-1, the 3-diketone.
(3b): will (1S, 2S, 4R)-2-two ring [2.2.1] heptan-2-base isoindole-1, the 3-diketone (1.2g, 5mmol) and a hydrazine hydrate (300mg, 6mmol) mixture in 10mL methyl alcohol refluxed 4 hours, was concentrated into dried then.Extract with methylene dichloride (2x100mL), and solids removed by filtration.The evaporation methylene dichloride obtain 300mg (54%) (1S, 2S, 4R)-norcamphyl-2-amine.
Intermediate embodiment 9Outward-two the ring [2.2.1] heptan-5-alkene-2-amine
Adopt the method identical with intermediate embodiment 12, by 5-norbornylene-2-alcohol through the multi-usage intermediate outside-2-two rings [2.2.1] heptan-5-alkene-2-base isoindole-1, outside the preparation of 3-diketone-two encircle [2.2.1] heptan-5-alkene-2-amine.
Intermediate embodiment 102-methyl-6,7-dimethoxy quinoxaline
Title compound adopts people's such as improved Tamao method (tetrahedron, 1982,38,3347-3354) preparation.。Under the argon atmospher, in THF solution, add 2-chloro-6, and 7-dimethoxy quinoxaline (5g, 26mmol) and NiCl 2(dppp) (0.14g, 0.26mmol).Reaction mixture to 0 ℃, add in batches 3M MeMgBr diethyl ether solution (13mL, 39mmol).Warm reaction mixture stirred 1 hour to room temperature, further refluxed then 1.5 hours.Cooling mixture is used the 10%HCl quenching, and stirs 10 minutes, is adjusted to alkalescence with 5%NaOH subsequently.In reactant, add methylene dichloride and water, stir the mixture and spend the night.And then add CH in addition 2Cl 2, H 2O and NaCl, filtering mixt.Gained solution is poured in the separating funnel into water layer washed with dichloromethane 3 times.Merge organic layer, use the salt water washing, dry (MgSO 4), enriched material is poured on the silica gel, and carries out chromatography (50%-80%EtOAc/ hexane), obtains orange solids (49% yield).
Intermediate embodiment 116,7-dimethoxy-2-quinoxaline formaldehyde
Under the argon atmospher, in reaction flask, add 1,4-diox (20mL), 2-methyl-6,7-dimethoxy quinoxaline (1.09g, 5.3mmol) and SeO 2(1.8g, 16mmol).Heated mixt to 100 ℃ reaction 2 hours 45 minutes, cooling, and pass through diatomite filtration.Filter bed is with several parts of ethyl acetate and washed with dichloromethane.Concentrate gained solution, residue is dissolved in MeOH/CH 2Cl 2, and be contained in and carry out chromatography (30%EtOAc/CH on the silicagel column 2Cl 2), obtain pale solid (73% yield).
Intermediate embodiment 12(outside 2,5 is outer)-5-amino bicyclic [2.2.1] heptane-2-acetic ester
(J.Chem.Soc.Perkin trans I.1505 according to the method for improved slightly R.Gagnon, 1995), by two ring [2.2.1] heptan-2, outside the preparation of 5-diene-5-acetoxyl group two ring [2.2.1] heptane-2-ketone and outer-6-acetoxyl group two ring [2.2.1] heptane-2-ketone.
Under the room temperature, outwards-5-acetoxyl group two ring [2.2.1] heptane-2-ketone (350mg, and adding 1M borine/THF solution in 10mL THF solution 2.08mmol) (1.2mL, 1.2mmol).Stirred the mixture 0.5 hour, and under 0 ℃, added methyl alcohol (3mL) and 1N HCl (1.5mL) quenching then.Extract with ethyl acetate (3x30mL), and with dried over mgso.Filter and concentrate back gained residue by silica gel column chromatography, obtain (in 2,5 is outer)-5-acetoxyl group two ring [2.2.1] heptane-2-alcohol.
Under 0 ℃, to (in 2,5 is outer)-5-acetoxyl group two ring [2.2.1] heptane-2-alcohol (350mg, 2.06mmol) THF (10mL) solution in add phthalic imidine (454mg, 3.09mmol), triphenyl phosphine (810mg, 3.09mmol) and the diethylazodicarboxylate (0.49mL, 3.09mmol).Stirring reaction spends the night, and concentrates with rotatory evaporator then, and residue is passed through column chromatography purifying (20% ethyl acetate/hexane), obtains required product, is yellow solid.
With above-mentioned solid (300mg, 1mmol) and hydrazine (0.126mL, 2.2mmol) mixture heating up in 5mL methyl alcohol refluxed 6 hours.After removing methyl alcohol, extract residue with methylene dichloride (3x30mL).Obtaining behind the concentrated solvent (outer, outer)-5-amino bicyclic [2.2.1] heptane-2-acetic ester (127mg, 75%), it need not into-and go on foot purifying and be directly used in the linked reaction.
Similarly, can be by suitable initial substance preparation (in 2,5 is outer)-5-amino bicyclic [2.2.1] heptane-2-acetic ester, (in 2,6 is outer)-6-amino bicyclic [2.2.1] heptane-2-acetic ester and (outside 2,6 is outer)-6-amino bicyclic [2.2.1] heptane-2-acetic ester.
Intermediate embodiment 132-methoxyl group-4,5-diaminophenol dihydrochloride
Exist according to Ehrlich etc. The organic chemistry magazine(J.Org.Chem.), 1947,12, the method described in 522, hydrogenation 2-methoxyl group-4,5-dinitrophenol(DNP), preparation title compound.
Intermediate embodiment 14The pure and mild 6-hydroxyl of 7-hydroxyl-6-methoxyl group-quinoxaline-2--7-methoxyl group-quinoxaline-2-alcohol
Utilize the method for intermediate embodiment 2, by reacting with NaOH and glyoxylic acid ethyl ester, by 4-methoxyl group-5-hydroxybenzene-1,2-diamines dihydrochloride prepares title compound.
Intermediate embodiment 157-hydroxyl-6-methoxyl group-2-chloro-quinoxaline and 6-hydroxyl-7-methoxyl group-2-chloro-quinoxaline
Utilize the method for intermediate embodiment 3, by with POCl 3Reaction is by the pure and mild 6-hydroxyl of 7-hydroxyl-6-methoxyl group-quinoxaline-2--7-methoxyl group-quinoxaline-2-alcohol preparation title compound.
Formula I compound as herein described can suppress the generation of cell proliferation and/or cell matrix by suppressing the PDGF-R tyrosine kinase activity and/or cell moves (chemotaxis).The excessive generation of uncontrolled breeding of this cell or matrix or regulate and control bad apoptosis and cause multiple disease.These morbid states relate to the broad variety cell, and comprise some illnesss like this, as leukemia, cancer, glioblastoma, psoriasis, diseases associated with inflammation, osteopathy, fibrotic conditions, the restenosis of atherosclerosis and coronary artery, femoral artery or Renal artery postangioplasty or, as the sacroiliitis in the fibroproliferative disease, the fibrosis of lung, kidney and liver.Particularly, it is reported that PDGF is relevant with particular types cancer and tumour with PDGF-R, as the cancer of the brain, ovarian cancer, colorectal carcinoma, prostate cancer, lung cancer, kaposi's sarcoma and malignant melanoma.In addition, also has non-control sexual cell proliferative disease due to the coronary bypass-forming operation.It is believed that excessive generation that suppresses uncontrolled breeding of tyrosine kinase activity pair cell or matrix or the control of the apoptosis that is difficult to regulate and control are very effective.
The present invention relates to adjustment and/or inhibition cell signaling, cell proliferation and/or extracellular matrix generation and/or cell and move the method for (chemotaxis), also relate to the method that growth of control abnormity cell and cellular inflammation are replied.More specifically say, the present invention relates to the purposes of substd quinolines and quinoxaline compounds, by suppressing Thr6 PDGF BB-acceptor (PDGF-R) tyrosine kinase activity effectively, these compounds discharge differentiation, propagation, matrix generation, chemotaxis or medium and have selective inhibitory.
Autophosphorylation, promptly substrate host's phosphorylation in the phosphorylation of growth factor receptors itself and the born of the same parents can cause some and relate to the biochemical event that cell signaling, cell proliferation, cell matrix generation, chemotaxis and medium discharge.
By suppressing the Lck tyrosine kinase activity effectively, The compounds of this invention can also be used for the treatment of transplants resistance disease and autoimmune disease such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematous, transplant rejection, graft versus host disease (GVH disease), hyper-proliferative venereal disease disease such as tumour and psoriasis, and wherein cell is accepted the disease of pro-inflammatory signal, as asthma, inflammatory bowel and pancreatitis.When the resistance disease was transplanted in treatment, The compounds of this invention can preventatively use, and perhaps according to subject people the adverse effect situation of transplant organ or tissue was used.When preventative use, The compounds of this invention can be bestowed the patient or be applied on the tissue or organ that will transplant before transplantation.Prophylactic treatment also is included in after the transplantation but administration before observing any sign that transplanting is had adverse effect.When using according to the situation of adverse effect, The compounds of this invention can directly be bestowed the patient, so that resistance is transplanted in treatment after demonstrating resistance surface sign.
On the other hand, the present invention also provides treatment to suffer from or easily suffer from the patient's of more such illnesss method, wherein said illness can improve by the inhibitor of using PDGF-R tyrosine kinase activity inhibitor and/or Lck tyrosine kinase activity or prevent, for example, illness as indicated above, this method comprises to be used the formula I compound of significant quantity or contains formula I compound compositions the patient, or its pharmacologically acceptable salt.
Should be appreciated that treatment described here comprises prophylactic treatment and to confirming treatment of conditions.
In its scope, the present invention also comprises pharmaceutical composition, but said composition comprises at least a formula I compound and the pharmaceutically acceptable carrier of receiving amount on the medicine, adjuvant for example, thinner, dressing thing and vehicle.
In practice, the The compounds of this invention or the composition of treatment usefulness can any suitable form be used by for example suction, part, non-enteron aisle, rectum or oral route, and preferred oral is used.More specifically route of administration comprise in intravenously, intramuscular, subcutaneous, intraocular, the synovial membrane, colon, intraperitoneal, through the epithelium approach comprise through skin, through eye, hypogloeeis, in cheek, transdermal, eyeball, through insufflation and in the nose of aerosol form inhalation.
Formula I compound can be and be fit to the form that optimum allows administration.The invention still further relates to the pharmaceutical composition that contains at least a The compounds of this invention, they are suitable as the medicine that uses for the patient.These compositions can use one or more pharmaceutically acceptable adjuvants or vehicle preparation according to ordinary method.Adjuvant particularly comprises thinner, sterile aqueous media and various nonpoisonous organic solvent.In order to obtain pharmaceutically acceptable preparation, composition can be tablet, pill, granule, pulvis, the aqueous solution or suspension, injection liquid, elixir or syrup form, and can contain one or more be selected from sweeting agent (as sucrose, lactose, fructose, asccharin and
Figure A20081010053600591
), the material of seasonings (as spearmint oil, wintergreen oil, or cherry or orange condiment), tinting material and stablizer (as Tegosept M or propyl ester).
The selection of carrier and the active substance content in carrier generally depends on the condition of being observed in the solvability of product and chemical property, concrete administering mode and the pharmacy practice usually.For example, with vehicle (as lactose, Trisodium Citrate, lime carbonate, secondary calcium phosphate) and disintegrating agent (as starch, alginic acid and some compound silica gel) and lubricant (as Magnesium Stearate, sodium lauryl sulphate and talcum) mix, can be used for preparing tablet, lozenge, pill, capsule etc.For the preparation capsule, more advantageously use lactose and liquid vehicle, as high molecular weight polyethylene glycol.The physical form that many other materials can be used as the dressing thing or are used to modify dose unit.For example, tablet, pill or capsule can be used shellac, sucrose or their the two dressings.When using aqeous suspension, the material that they can contain emulsifying agent or help to suspend.Can also use thinner such as sucrose, ethanol, how pure as polyoxyethylene glycol, propylene glycol and glycerine, and chloroform or their mixture.In addition, active compound also can join and continue in delivery article and the preparation.
For oral administration, activating compounds can be used with for example inert diluent or with assimilable edible carrier, perhaps can be encapsulated in hard or the soft shell gelatin capsules, perhaps can also be pressed into tablet or can directly be incorporated in the food, perhaps can be mixed together and use can absorb forms such as tablet, buccal tablet, lozenge, capsule, elixir, suspension, syrup, wafer with vehicle.
For parenterai administration, can use The compounds of this invention at vegetables oil (for example sesame oil, peanut oil or sweet oil), or water-organic solution (as water and propylene glycol), injectable organic ester such as ethyl oleate, and the emulsion in the aseptic aqueous solution of pharmacologically acceptable salt, suspension agent or solution.Injectable forms must be the liquid that reaches easy injection degree, and can by for example use dressing thing (as Yelkin TTS), by keeping required particulate size (with regard to dispersion liquid) and by utilizing tensio-active agent to keep suitable flowability.By the absorption of using delayed absorption agent (for example, aluminum monostearate and gelatin) can prolong Injectable composition.The solution of the salt of product of the present invention is particularly suitable for intramuscular or subcutaneous injection administration.By in water, suitably mixing, can prepare the solution of the active compound of the present invention of free alkali or its pharmaceutical acceptable salt with tensio-active agent such as hydroxypropylcellulose.Also can in ethylene glycol, liquid macrogol or its mixture and oil, prepare dispersion liquid.The aqueous solution (also comprising the solution of salt in pure distilled water) can be used for intravenous administration, but condition is suitably to regulate their pH, suitably cushion and give isotonicity with capacity glucose or sodium-chlor, and carry out sterilization by heating, radiation, millipore filtration and/or with various sterilant and mycocide (for example Tegosept E, butylene-chlorohydrin, phenol, Sorbic Acid, Thiomersalate etc.).
Aseptic injectable solution can be prepared as follows: the active compound of requirement is blended in the appropriate solvent that contains above-mentioned various other compositions, if desired, carries out sterile filtration subsequently.In general, dispersion liquid can prepare by various sterile active compositions are incorporated in the sterile carrier, comprises basic dispersion medium and required above-mentioned other composition in the wherein said sterile carrier.With regard to the sterilized powder that is used to prepare aseptic injectable solution, its preferred manufacturing procedure comprises vacuum-drying and freeze drying technology, thereby produces the pulvis that activeconstituents adds any supplementary component required in aforementioned its sterile filtration solution.
For topical, can use the gel (water base or alcohol radical) that contains The compounds of this invention, creme or ointment.The compounds of this invention also can be incorporated in the gel or matrix that patch uses, thereby can pass through transdermal barrier slowly-releasing The compounds of this invention.
For inhalation, The compounds of this invention can be dissolved or suspended in be fit to use in atomizer or suspension or the solution aerosol suitable carrier in, perhaps can make its absorption or be adsorbed on the suitable solid carrier that Diskus uses.
The solids composition that rectal administration is used comprises according to the suppository of known method preparation, wherein contains at least a formula I compound.
Like this preparation present composition, so that can resist the rapid removing situation from vascular (artery or the vein) wall by convection current and/or due to spreading, thereby the increase virion is at the residence time of required site of action.The adventitia week Drug Storage (depot) that comprises The compounds of this invention can be used for continuing to discharge.A kind of so useful Drug Storage that is used to use The compounds of this invention can be copolymer matrix (as an ethane-acetic acid ethyenyl ester), or is coated with the polyvinyl alcohol of Silastic shell.On the other hand, The compounds of this invention also can the silicone polymer in being implanted in adventitia the part disengage.
In skin, intravascular delivery process, another minimum alternative approach is reduced in the loss of The compounds of this invention comprise use non-dispersive medicament elution type microparticle.These microparticles can comprise various synthetic polymers, as polyactide, or crude substance, comprise protein or polysaccharide.This class microparticle can in a planned way be controlled the various variablees that comprise medicine total dose and release dynamics thereof.Microparticle can be injected into artery or wall of vein effectively by the air bag on porous balloon conduit or the stent, and keep at least two weeks at vessel wall with in organizing in adventitia week.In the local vascular preparation of locus specificity delivering therapeutic agents and methodology see people such as Reissen described ( American heart institute magazine(J.Am.Coll.Cardiol.) 1994,23:1234-1244), its full content is incorporated into incorporated by reference at this.
The present composition also can comprise hydrogel, and this hydrogel absorbs the hydrophilic polypropylene acid polymer of sponge by (all or assorted) polymers preparation of any biocompatibility or non-cell toxicity as useful as drug.For example, these polymkeric substance have introduction in patent application WO 93/08845, and its full content is incorporated into incorporated by reference at this.In them some, for example, particularly those that are made by ethylene oxide and/or propylene oxide can be buied from the market.
When using The compounds of this invention to treat the pathology disease relevant, can use The compounds of this invention by different modes with hyper-proliferative venereal disease disease.Treatment for restenosis, can utilize the angioplasty air bag (the combined thing of hydrophilic film wherein is saturated) that is coated with hydrophilic film (for example hydrogel), perhaps utilize any other conduit that contains compound infusion chamber, The compounds of this invention is directly bestowed the vessel wall place, thereby can be applied in accurate mode and be subjected to therapentic part, and discharge compound in the part effectively being treated the cell position.More preferably, this medication can make compound promptly with the cells contacting of needs treatment.
The advantage of methods of treatment of the present invention is be subjected to import The compounds of this invention on the therapentic part.For example, the composition that comprises hydrophilic gel directly can be deposited on the surface that is subjected to therapentic part (for example, in the surgical intervention process just can so).Advantageously, preferably in the angioplasty process, hydrophilic gel is by being coated on conduit (for example balloon catheter) and going up and can import to desirable internal blood vessel position, and is discharged in the vessel wall.In a kind of particularly advantageous mode, utilize balloon catheter that saturated hydrophilic gel is imported to and be subjected to therapentic part.After in conduit is inserted into blood flow, minimum for the medicine loss is reduced to, can use air bag, because conduit is towards target vessel direction progressive with the protectiveness sheath.
Another embodiment of the present invention provides utilizes the perfusion air bag to use The compounds of this invention.These perfusion air bags may can make blood flow be remained unchanged, when airbag inflation, just reduced the danger of myocardial ischaemia like this, (above 20 minutes) local release compound in also can be under normal pressure long-time relatively, and this long-time also be essential for its best use of.On the other hand, can also use the groove balloon catheter (" groove balloon angioplasty conduit ", Mansfield Medical, Boston Scientific Corp., Watertown, MA).The latter comprises the conventional air bag that is coated with 24 hole perforated tube layers, pours into these perforated tubes by additional infusion orifice through chamber independently.Reissen etc. (1994) have introduced various types of balloon catheters, as two air bags, porous balloon, the micropore air bag, passage air bag, the stent and the hydrophilic gel conduit of band air bag, all these can be used for implementing the present invention, and the full content of above-mentioned document is incorporated into incorporated by reference at this.
Especially preferably use the gas injection ductus bursae, can keep the advantage of airbag inflation because it not only has by keeping sliding properties easily for a long time, but also have the site-specific advantage of hydrophilic gel, these two kinds of advantages can obtain simultaneously.
Another aspect of the present invention relates to and comprises that (as poloxamer 407 are the nontoxic how pure of a kind of biocompatibility, are commercially available (BASF, Parsippany, NJ)) pharmaceutical composition for The compounds of this invention and poloxamer.
The poloxamer that is impregnated with The compounds of this invention for example can directly be deposited on the surface that is subjected to treated tissue during the surgical intervention.Poloxamer has and the essentially identical advantage of hydrophilic gel, but viscosity is lower.
It is particularly favourable that use has the groove balloon catheter of the impregnated poloxamer of The compounds of this invention.In this case, not only have the long-term maintenance airbag inflation advantage of (can keep the characteristic of slip easily simultaneously), but also have the site-specific advantage of poloxamer, these two kinds of advantages can obtain simultaneously.
The percentage composition of activeconstituents in the present composition is variable, but essential formation obtains the required ratio of optimal dose.Obviously, can use several unit dosage forms in about same time.Used dosage is determined by attending doctor or qualified doctor, and depends on required curative effect, route of administration and treatment time length and patient's the state of an illness.For the adult, the dosage of inhalation is generally the about 50mg of the about 0.001-of every kg body weight every day, the preferred about 5mg of about 0.001-, oral administration dosage is about 0.01-about 100, preferred 0.1-70, the dosage of more preferably 0.5-10mg/kg body weight, and intravenous administration is about 10 for about 0.001-, preferably the 0.01-10mg/kg body weight.In various concrete cases, will determine used dosage as age, body weight, general health and other character that can influence The compounds of this invention effectiveness according to the specific factor of being treated the patient.
For obtaining required curative effect, can use The compounds of this invention/composition as required continually.Some patients may can seek out lower suitable maintenance dose thus to higher or make rapid reaction than low dosage.For other patient, according to each concrete patient's physiological requirements, may with every day 1-4 dosage administration frequency carry out long-term treatment.Usually active result Orally-administrable every day is 1-4 time.Certainly, to other patient, it may be essential once or twice that regulation administration every day is no more than.
The compounds of this invention also can use with other therapeutical agent such as medicament co-formulated, perhaps as mentioned below, so prepare them so that be used for described pharmacology illness position with the treatment technology combination, this can be alleviated illness by using formula I compound:
In the process that adopts any device treatment postangioplasty restenosis of using such as air bag, part excision or laser technology, can use The compounds of this invention.1) blood vessel sealing is tentatively treated, or 2) can not obtain under the situation of open artery in the angioplasty that adopts any device, The compounds of this invention be used for the treatment of restenosis after can be in stent is placed in vascular system.The compounds of this invention can use by oral, parenterai administration mode, and perhaps The compounds of this invention can be by the intervention of extraordinary device or with the suitable preparation coating form topical application on the stent device.
The compounds of this invention can be united with any antithrombotics, anti-platelet agents, antithrombotic agent or short solution fibrin agent and is used for the treatment of restenosis.In order to carry out interventional therapy safely or to prevent thrombotic deleterious effect, usually before the interventional therapy, among and use these kind medicaments that the patient is carried out concurrent treatment afterwards.The example that is known as some medicament kind of antithrombotics, anti-platelet agents, antithrombotic agent or short solution fibrin agent comprises any preparation of heparin, low molecular weight heparin, pentasaccharides, fibrinogen deceptor antagonists, thrombin inhibitors, Xa factor inhibitor, or VIIa factor inhibitors.
The compounds of this invention can be united use to treat hypertension or atherosclerosis simultaneously with any antihypertensive drug or cholesterol or lipid regulating agent when treatment restenosis or atherosclerosis.Some examples that can be used for treating hypertensive medicament comprise following kind: beta-Blocking agent, ACE inhibitor, calcium-channel antagonists and alpha-receptor antagonist.Some examples that can be used for treating the medicament of elevated cholesterol or lipid level imbalance comprise the compound that is known as the HMGCoA reductase inhibitor, sodium pentosan polysulfate class (fibrate) compound.
The compounds of this invention can be separately or is united with the known compound that can be used for treating cancer and to be used for the treatment of the various forms cancer.
Should be appreciated that the binding substances that the present invention includes The compounds of this invention and one or more mentioned kind therapeutical agents.
According to the described test method of document, the compound exhibits in the scope of the invention goes out significant pharmacologically active, it is believed that these test-results are relevant with other mammiferous pharmacologically active with the people.In the following body and the in-vitro pharmacological experiments result be the characteristic feature of The compounds of this invention.
Preparation of drug combination and pharmacological testing part
Compound in the scope of the invention demonstrates remarkable activity as protein tyrosine kinase inhibitors, and as the cellular antiproliferative agent some treatment of conditions that comprises psoriasis, atherosclerosis and restenosis damage is had therapeutic value.Compound exhibits in the scope of the invention goes out to regulate and/or suppress the activity of cell signaling and/or cell proliferation and/or matrix generation and/or chemotaxis and/or cell inflammatory response, and can be used for preventing or delay the generation or the recurrence of these illnesss or be used for the treatment of these illnesss.
In order to determine the effectiveness of The compounds of this invention, used pharmacological testing hereinafter described, these tests are recognized in the art, and think relevant with mammiferous pharmacologically active.Compound in the scope of the invention is carried out these different tests, it is believed that the gained result is relevant with effective cytodifferentiation medium activity.Can be sure of that for the professional in pharmacology and medical chemistry field, these test-results use the parameter of test-compound that technology for full information is provided for determining in one or more therapies as herein described.
1. The enzyme-linked immunosorbent assay of PDGF-R Tyrosylprotein kinase autophosphorylation
Title measure by described methods such as Doll carry out ( The pharmaceutical chemistry magazine(J.Med.Chem.) 1994,37,2627) (its content is incorporated into incorporated by reference at this), but use the cell lysate of following derived from human aortic smooth muscle cell (HAMSC).
2. The general method of mensuration takes place in cell fission
A. cell cultures
Human aortic smooth muscle cell's (4-9 generation) is supported to cultivate in the substratum in growth in 96 well culture plates, and every hole 6000 cells were grown 2-3 days.Reach about 85% converge after, utilize serum free medium (SFM), make the cell cessation of growth cessation.
B. cell fission is measured
Serum is removed nutrient solution after consuming poor 24 hours, replaces with the SFM solution (200 μ l/ hole) of test-compound/carrier.Compound adds and is dissolved among the DMSO that cell cultures uses, and concentration 10mM further dilutes in SFM then.
With the compound preincubate after 30 minutes, with 10ng/mL PDGF irritation cell.Under each compound concentration,, duplicate to stimulating and not stimulating the hole to measure.
After 4 hours, add 1 μ Ci 3H thymidine/hole.
Termination in 24 hours is cultivated after adding somatomedin.Use trypsin digestion and cell, and utilize automated cell results instrument (Wallac Mach1196) with cell harvesting on filter membrane.Filter membrane is measured the marker that mixes DNA with scintillation counter counting (Wallac Betaplate).
3. Chemotaxis is measured
The human aortic smooth muscle cell (HASMC) of being gone down to posterity in early days from ATCC.Cell is cultivated in Clonetics SmGM2 SingleQuots substratum, used 4-10 for cell.After treating that cell 80% converges, in nutrient solution, add fluorescent probe calcein AM (5mM, molecular probe), and incubation cell 30 minutes.After with the washing of HEPES buffer saline, the cell tryptic digestion, and neutralize with MCDB 131 damping fluids (Gibco) that contain 0.1%BSA, 10mM glutamine and 10% foetal calf serum.After centrifugal, washed cell once is resuspended in 3000 cells/50mL in the same buffer of no foetal calf serum again.With different concns formula I compound (final DMSO concentration=1%) in 37 ℃ of incubation cells 30 minutes.For chemotaxis research, use 96 holes improve the Boyden groove (Neuroprobe, Inc.) and the polycarbonate membrane in 8mm aperture (Poretics, CA).(Sigma C3657 0.1mg/mL) applies described film with collagen protein.PDGF-β β (3ng/mL)/damping fluid (wherein containing or do not contain formula I compound) is placed in the low groove, and the cell (30,000) that contains or do not contain inhibitor places in the high groove.Incubation cell 4 hours.Remove filter membrane, and remove the cell of going up on the film side.After the drying, utilize Cytofluor II (Millipore) to measure the fluorescence intensity of film at 485/530nm excitation/emission wavelength place.In each test, obtain average cell migration value by six replicate(determination)s.Determine percent inhibition according to the contrast class value that DMSO handles.According to 5 concentration dependent inhibiting values, calculate IC 50Value.The result represents with the mean value ± SEM of five groups of this tests.
4. EGF-acceptor purifying
The purifying of EGF-acceptor is based on the method for Yarden and Schlessinger.The A431 cell is at 80cm 2Grow in the bottle and converge (2x10 7Cell/bottle).With PBS washed cell twice, and with the PBS results (37 ℃ are following 1 hour) that contain 11.0mmol EDTA, with 600g centrifugal 10 minutes.Under 4 ℃, with cell at cold solubilization damping fluid (50mmol Hepes damping fluid, pH 7.6,1% triton x-100s, 150mmol NaCl, 5mmol EGTA, 1mmol PMSF, 50mg/mL aprotinin, 25mmol benzamidine, 5mg/mL leupeptin and 10mg/mL Trypsin inhibitor SBTI) with every 2x10 7Cell 1mL solubilization 20 minutes.With 100,000g is after centrifugal 30 minutes, with supernatant liquor WGA-agarose column (the every 2x10 that packs into 7Cell 100mL potting resin), under 4 ℃, shook 2 hours.Remove not adsorptive, resin HTN damping fluid (50mmol HepeS, pH 7.6,0.1% triton x-100s, 150mmol NaCl) washed twice, the HTN damping fluid washed twice that contains 1M NaCl, use HTNG damping fluid (50mmol Hepes, pH7.6,0.1% triton x-100 again, 150mmol NaCl and 10% glycerine) washed twice.EGF-acceptor HTNG damping fluid (the every 2x10 that contains 0.5M N-ethanoyl-D-glucosamine 7Cell 200mL) wash-out in batches.Eluate waits the increment form to be stored in-70 ℃ with several parts, faces with preceding with TMTNG damping fluid (50mmol Tris-Mes damping fluid, pH7.6,0.1% triton x-100,150mmol NaCl, 10% glycerine) dilution.
5. Inhibition to the effect of EGF-R autophosphorylation
Cell is grown on the tissue culture ware of people's fibronectin coating to be converged.After ice-cold PBS washed twice, every culture dish adds the 500mL lysis buffer, and (50mmol Hepes, pH 7.5,150mmol NaCl, 1.5mmol MgCl 2, 1mmol EGTA, 10% glycerine, 1% triton x-100,1mmol PMSF, 1mg/mL aprotinin, 1mg/mL leupeptin) and lysing cell, and 4 ℃ of incubations 5 minutes.Stimulate (500mg/mL, 10 minutes, 37 ℃) afterwards at EGF, carry out immunoprecipitation with anti-EGF-R (Ab 108), and 2 or the 10mM The compounds of this invention in the presence of carry out autophosphorylation in 4 ℃ and react (50mL equal portions liquid, 3mCi[g- 32P] ATP) sample 2 minutes.Add thermoelectric swimming sample buffer termination reaction.SDA-PAGE analyzes (7.5%els), then carries out radioautograph, by densitometric scan x radiographic film quantitative reaction.
A. cell cultures
By lacking the NIH3T3 cell (clone 2.2) of endogenous EGF-acceptor (available from C with the wild-type EGF-acceptor of no tyrosine kinase activity or the cDNA construct transfection of sudden change EGF-acceptor (wherein the Lys on the ATP-binding site 721 is replaced by the Ala residue respectively), Fryling, NCI, NIH), preparation is called the cell of HER14 and K721A.At the DMEM that contains 10% foetal calf serum (Hyclone, Logan, Utah) the middle all cells of cultivating.
6. Utilize the selectivity of commercial reagent box mensuration to PKA and PKC
A.Pierce colorimetry PKA assay kit, helicase form (Spinzyme Format) simple scheme:
PKA enzyme (OX-heart) 1U/ detector tube
Ken Pu (kemptide) peptide (dye marker) substrate
45 Fen Zhong @30 ℃
The optical density at 570nm place
B.Pierce colorimetry PKA assay kit, helicase form (Spinzyme Format) simple scheme:
PKC enzyme (rat brain) 0.025U/ detector tube
Neurogranin peptide (dye marker) substrate
45 Fen Zhong @30 ℃
The optical density at 570nm place
7. P56 Lck Tyrosylprotein kinase suppresses active mensuration
Press United States Patent (USP) 5,714,493 described methods are measured p56 LckThe tyrosine-kinase enzyme inhibition activity, described literature content is incorporated into incorporated by reference at this.
In another alternative method, the tyrosine-kinase enzyme inhibition activity is measured as follows.Under the room temperature, substrate (substrate that contains tyrosine, i.e. p56 LckDiscernible Biot-(β Ala) 3-Lys-Val-Ile-Gly-Glu-Gly-Thr-Tyr-Glu-Val-Val-Tyr-Lys-(NH 2), 1 μ M) having or do not having in the presence of the given concentration test compound, (enzyme is by expressing p56 in the yeast construct through the enzyme of clone's yeast purifying with specified rate LckGene produces, and its purifying is finished according to following classical way) at ATP (10 μ M), MgCl 2(2.5mM), MnCl 2(2.5mM), NaCl (25mM), DTT (0.4mM), at Hepes 50mM, there are following phosphorylation for the first time 10 minutes in pH 7.5.Total reaction volume is 50 μ l, is reflected in the 96 hole black fluorescent plates to carry out.Add 150 μ l and contain stop buffer (the 100mM Hepes pH 7.5 of the Streptavidin (XL665) of the selected anti-tyrosine antibody of europium kryptofix 222 (PY20-K) (0.8 μ g/ml dosage) mark and allophycocyanin (4 μ g/ml) mark, KF 400mM, EDTA 133mM, BSA 1g/l) termination reaction.The mark of Streptavidin and anti-tyrosine antibody is finished by Cis-BioInternational (France).The Packard Discovery calculating instrument counting mixture (excitation wavelength 377nm reads at 620nm and 665nm place) that uses energy measurement temporal resolution homogeneous fluorescent energy to shift.The ratio of 665nm signal/620nm signal is measuring of phosphorylated tyrosine concentration.Replace enzyme with damping fluid, obtain blank.The difference of the ratio that obtains under the ratio that obtains under the unrestraint agent situation and the blank situation is for than signal.Calculate % and compare signal.Use Xlfit software, calculate IC by 10 kinds of concentration inhibitor (in duplicate) 50Value.Reference compound is Staurosporine (Sigma), its IC 50Be 30 ± 6nM (n=20).
The result of above-mentioned experimental technique shows that the compound in the scope of the invention has effective pdgf receptor protein tyrosine kinase and suppresses activity or p56 LckThe tyrosine-kinase enzyme inhibition activity, thereby have therapeutic value.For selected concrete treatment, the result of above-mentioned pharmacological testing can be used for determining dosage and administering mode.
Under the situation that does not deviate from spirit of the present invention or base attribute, the present invention can implement with other particular form.

Claims (64)

1. formula I compound or its N-oxide compound, its hydrate, its solvate, its prodrug or its pharmacologically acceptable salt:
Figure A20081010053600021
Wherein:
X is L 1Or L 2Z 2
L 1Be (CR 3aR 3b) rH or (CR 3aR 3b) m-Z 3-(CR 3 ' aR 3 ' b) nH;
L 2Be (CR 3aR 3b) p-Z 4-(CR 3 ' aR 3 ' b) q
Z 1Be CH or N;
Z 2Be to replace and unsubstituted cycloalkyl, wherein said cycloalkyl be meant contain 3-10 carbon atom non-aromaticity singly-or encircle ring system and cycloalkyl randomly by alkyl more, hydroxyl, acyloxy, alkoxyl group is the halogen of fluorine, chlorine, bromine, iodine, R 5R 6N-, acyl group R 5N-, carboxyl or R 5R 6The NCO-substituting group, or the divalence oxygen that is positioned at two adjacent carbonss (O-) replaces; Or replacement and unsubstituted cycloalkenyl group, wherein said cycloalkenyl group is meant the non-aromatic monocycle that contains carbon-to-carbon double bond and 3-10 carbon atom or encircles ring system more that cycloalkenyl group is randomly replaced by above-mentioned naphthenic substituent; Or replacement and unsubstituted heterocyclic, wherein said heterocyclic radical is meant 4-10 unit's monocycle or encircles ring system more that wherein heterocyclic radical is randomly replaced by the substituting group of above-mentioned cycloalkyl; Or replacement and unsubstituted heterocycloalkenyl, wherein said heterocycloalkenyl is meant 4-10 unit's monocycle of fractional saturation or encircles ring system more, one or more atoms are the non-carbon atom that is selected from nitrogen, oxygen or sulphur in the ring system, and heterocycloalkenyl is randomly replaced by the substituting group of above-mentioned cycloalkyl;
Z 3Be O, NR 4, S, SO or SO 2
Z 4Be O, NR 4, S, SO, SO 2
M is 0 or 1;
N is 2 or 3, and n+m=2 or 3;
P and q are 0,1,2,3 or 4 independently, and p+q=0,1,2 or 3;
R is 2,3 or 4;
R 1aAnd R 1bFor replacing and unsubstituted alkyl, wherein alkyl is side chain or the straight chain with 1 to 10 carbon atom independently, and alkyl is alkoxy randomly, halogen, carboxyl, hydroxyl or R 5R 6N-replaces; Or replacement and unsubstituted aryl, wherein aryl be contain the aromatic carbon ring group of 6-10 carbon atom and aryl optional by hydrogen, hydroxyl, halogen, alkyl, alkoxyl group, carbalkoxy or Y 1Y 2NCO-replaces, wherein Y 1And Y 2Be hydrogen or alkyl independently; Or replacement and unsubstituted heteroaryl, wherein heteroaryl is meant about 5-to about 10-fragrant monocycle of unit or polynuclear hydrocarbon ring system, and optional being replaced by the substituting group of above-mentioned aryl; Or hydroxyl; Acyloxy; Or replacement and unsubstituted alkoxyl group, wherein alkoxyl group is meant alkyl-O-group, wherein alkoxyl group is randomly replaced by amino, alkoxyl group, carboxyl, carbalkoxy, carboxyl aryl, formamyl or heterocyclic radical group; Or replacement and unsubstituted cycloalkyloxy, wherein cycloalkyloxy is meant cycloalkyl-O-group, wherein cycloalkyl is as mentioned above; Or replacement and unsubstituted heterocyclic oxy group, wherein heterocyclic oxy group is meant heterocyclic radical-O-group, heterocyclic radical wherein is as mentioned above; Or replacement and unsubstituted heterocycle carbonyl oxygen base, wherein heterocycle carbonyl oxygen base is meant heterocyclic radical-C (O) O-group, heterocyclic radical wherein as above defines; Or replacement and unsubstituted aryloxy, wherein aryloxy is meant aryl-O-group, what wherein aryl was optional is replaced by the substituting group at aryl as mentioned above; Or replacement and unsubstituted heteroaryloxy, wherein heteroaryloxy is meant heteroaryl-O-group, what wherein heteroaryl was optional is replaced by the substituting group at aryl as mentioned above; Or cyano group, R 5R 6N-or acyl group R 5N-, perhaps R 1aAnd R 1bOne of be hydrogen or halogen, and another is for replacing and unsubstituted alkyl;
R 1cBe hydrogen, Ding Yi replacement and unsubstituted alkyl replace and unsubstituted aryl as described above, replace and unsubstituted heteroaryl hydroxyl, acyloxy, replace and unsubstituted alkoxyl group, replace and unsubstituted cycloalkyloxy, replace and unsubstituted heterocyclic oxy group, replace and unsubstituted aryloxy, replace and unsubstituted heteroaryloxy, halogen, cyano group, R 5R 6N-or acyl group R 5N-;
R 3a, R 3b, R 3 ' aAnd R 3 ' bBe hydrogen or alkyl independently;
R 4Be hydrogen, alkyl or acyl group; With
R 5And R 6Be hydrogen or alkyl, perhaps R independently 5And R 6Form azaheterocyclyl with the nitrogen-atoms that they connected.
2. the compound of claim 1 or its N-oxide compound, its hydrate, its solvate, its prodrug or its pharmacologically acceptable salt, wherein:
L 1Be (CR 3aR 3b) m-Z 3-(CR 3 ' aR 3 ' b) n
L 2Be (CR 3aR 3b) p-Z 4-(CR 3 ' aR 3 ' b) q
Z 2For replacing and unsubstituted cycloalkyl, replace and unsubstituted cycloalkenyl group or replacement and unsubstituted heterocyclic;
Z 4Be O and NR 4
M is 0;
N is 2 or 3,
P+q=0 or 1;
R 1aAnd R 1bDefinition for replacing and unsubstituted alkyl, replaces and unsubstituted alkoxyl group independently as described above, replaces and unsubstituted cycloalkyloxy, replaces and unsubstituted heterocyclic oxy group or R 5R 6N-, perhaps R 1aAnd R 1bOne of be hydrogen or halogen, and another is for replacing and unsubstituted alkyl, replaces and unsubstituted alkoxyl group, replaces and unsubstituted cycloalkyloxy, replacement and unsubstituted heterocyclic oxy group or R 5R 6N-;
R 1cBe hydrogen, define replacement and unsubstituted alkyl or replacement and unsubstituted alkoxyl group as described above;
R 3a, R 3b, R 3 ' aAnd R 3 ' bBe hydrogen or low alkyl group independently;
R 4Be hydrogen; With
R 5And R 6Form azaheterocyclyl with the nitrogen-atoms that they connected.
3. the compound of claim 1, or its N-oxide compound, its hydrate, its solvate, its prodrug or its pharmacologically acceptable salt, wherein:
X is L 2Z 2
L 2Be (CR 3aR 3b) p-Z 4-(CR 3 ' aR 3 ' b) q
Z 2Replace and unsubstituted cycloalkyl or replacement and unsubstituted cycloalkenyl group for defining as described above;
Z 3Be O and NR 4
P is 0;
Q is 0 or 1;
R 1aAnd R 1bDefinition for replacing and unsubstituted alkyl, replaces and unsubstituted alkoxyl group independently as described above, replaces and unsubstituted cycloalkyloxy or replacement and unsubstituted heterocyclic oxy group, perhaps R 1aAnd R 1bOne of be hydrogen or halogen,
R 1cBe hydrogen;
R 3 ' aAnd R 3 ' bBe hydrogen independently; With
R 4Be hydrogen.
4. the compound of claim 1, wherein L 1Be low alkyl group.
5. the compound of claim 1, wherein Z 1Be CH.
6. the compound of claim 1, wherein Z 1Be N.
7. the compound of claim 1, wherein Z 2Replacement and unsubstituted cycloalkyl for definition as described above.
8. the compound of claim 1, wherein Z 2Monocyclic cycloalkyl for the alkyl replacement.
9. the compound of claim 1, wherein Z 2Be methylcyclopentyl or methylcyclohexyl.
10. the compound of claim 1, wherein Z 2Be the polycyclic naphthene base.
11. the compound of claim 1, wherein Z 2Be [2.2.1] two suberyl (norcamphyl) or [2.2.1] two ring octyl groups.
12. the compound of claim 1, wherein Z 2Replacement and unsubstituted cycloalkenyl group for definition as described above.
13. the compound of claim 1, wherein Z 2Be cyclopentenyl and cyclohexenyl.
14. the compound of claim 1, wherein Z 2Be [2.2.1] bicycloheptene base (norbornene) or [2.2.2] bicyclooctene base.
15. the compound of claim 1, wherein p and q are 0.
16. the compound of claim 1, wherein p+q=1.
17. the compound of claim 1, wherein Z 4Be O.
18. the compound of claim 1, wherein Z 4Be O, and p and q are 0.
19. the compound of claim 1, wherein Z 4Be O, and p+q=1.
20. the compound of claim 1, wherein Z 4Be NR 4
21. the compound of claim 1, wherein Z 4Be NR 4, and p and q are 0.
22. the compound of claim 1, wherein Z 4Be NR 4, and m+n=1.
23. the compound of claim 1, wherein Z 4Be S.
24. the compound of claim 1, wherein Z 4Be S, and p and q are 0.
25. the compound of claim 1, wherein Z 4Be S, and p+q=1.
26. the compound of claim 1, wherein R 1aAnd R 1bBe hydroxyl replacement and unsubstituted low alkyl group, hydroxyl, lower alkoxy, cycloalkyloxy, heterocyclic oxy group, perhaps R independently 1aAnd R 1bOne of be hydrogen or halogen.
27. the compound of claim 1, wherein R 1aAnd R 1bBe heterocyclic radical carbonyl oxygen base or replacement and unsubstituted lower alkoxy independently.
28. the compound of claim 1, wherein lower alkoxy is a methoxy or ethoxy.
29. the compound of claim 1, wherein R 1aAnd R 1bBe low alkyl group.
30. the compound of claim 1, wherein low alkyl group is methyl or ethyl.
31. the compound of claim 1, wherein R 1aAnd R 1bOne of be lower alkoxy, and R 1aAnd R 1bIn another be halogen.
32. the compound of claim 1, wherein lower alkoxy is a methoxy or ethoxy, and halogen is a chlorine or bromine.
33. the compound of claim 1, wherein R 1aAnd R 1bOne of be low alkyl group, and R 1aAnd R 1bIn another be lower alkoxy.
34. the compound of claim 1, wherein lower alkoxy is a methoxy or ethoxy, and low alkyl group is methyl or ethyl.
35. the compound of claim 1, wherein R 1aAnd R 1bOne of be lower alkoxy, and R 1aAnd R 1bIn another be cycloalkyloxy.
36. the compound of claim 1, wherein lower alkoxy is a methoxy or ethoxy, and cycloalkyloxy is cyclopentyloxy or cyclohexyloxy.
37. the compound of claim 1, wherein R 1aAnd R 1bOne of be hydrogen, and R 1aAnd R 1bIn another be lower alkoxy, cycloalkyloxy or heterocyclic oxy group.
38. the compound of claim 37, lower alkoxy wherein are methoxy or ethoxy.
39. the compound of claim 37, cycloalkyloxy wherein are cyclopentyloxy or cyclohexyloxy.
40. the compound of claim 37, heterocyclic oxy group wherein are furans oxygen base.
41. the compound of claim 1, wherein R 1cBe hydrogen, low alkyl group or lower alkoxy.
42. the compound of claim 41, lower alkoxy wherein are methoxy or ethoxy.
43. the compound of claim 1, wherein R 1aAnd R 1bBe optional alkoxy, heterocyclic radical, carboxyl, the lower alkoxy that carbalkoxy or formamyl replace.
44. the compound of claim 1, wherein R 1aAnd R 1bOne of be unsubstituted lower alkoxy, and R 1aAnd R 1bIn another be the lower alkoxy that alkoxy, heterocyclic radical, carboxyl, carbalkoxy or formamyl replace.
45. the compound of claim 1, wherein R 1aAnd R 1bOne of be methoxyl group, and R 1aAnd R 1bIn another be [1,4 ']-Lian piperidines-1 '-Ji carbonyl oxygen base, 2-(oxyethyl group) oxyethyl group, 2-(4-morpholinyl) oxyethyl group, 2-(4-methylpiperazine-1-yl) oxyethyl group, carboxyl methoxyl group, the methoxycarbonyl methoxyl group, the aminocarboxyl methoxyl group, N-methylamino carbonyl methoxyl group or N, N-dimethylamino carbonyl methoxyl group.
46. according to the compound of claim 1, described compound is:
3-cyclohexyloxy-6, the 7-dimethoxy-quinoline;
2-cyclohexyl amino-6,7-dimethoxy quinoxaline;
Outward-two the ring [2.2.1] heptan-2-base-(6-chloro-7-methoxyl group quinoxaline-2-yl) amine;
Outward-two the ring [2.2.1] heptan-2-base-(7-chloro-6-methoxyl group quinoxaline-2-yl) amine;
Two the ring [2.2.1] heptan-2-base-(6,7-dimethyl quinoxaline-2-yl) amine;
2-suberyl amino-6,7-dimethoxy quinoxaline;
2-cyclopentyl amino-6,7-dimethoxy quinoxaline;
2-cyclohexyl amino-6-methoxyl group quinoxaline;
3-aminocyclohexyl-6, the 7-dimethoxy-quinoline;
(6,7-dimethoxy yl-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base) amine;
2-cyclohexyl amino-6-methoxyl group-7-bromo-quinoxaline hydrochloride;
(6,7-dimethoxy-quinoline-3-yl)-cis/trans-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-trans-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-cis-(3-(R)-methyl-cyclohexyl base)-amine;
(6,7-dimethoxy-quinoline-3-yl)-(3-methyl-cyclopentyl)-amine;
Hexamethylene-3-thiazolinyl-(6,7-dimethoxy quinoxaline-2-yl)-amine;
2,7-pair-cyclohexyloxy-6-methoxyl group-quinoxaline;
Cyclohexyl-(6,7-dimethoxy quinoxaline-2-ylmethyl)-amine;
(6,7-dimethoxy-quinoline-3-yl)-isobutylamine;
Cyclohexyl-(6-methoxyl group-7-morpholine-4-base-quinoxaline-2-yl)-amine;
(±)-two ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
Outward-two the ring [2.2.1] heptan-5-alkene-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
Cyclohexyl-(6,8-dimethyl-quinoxaline-2-yl)-amine;
In-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
(6,7-dimethoxy quinoxaline-2-yl)-(4-methoxyl group-cyclohexyl)-amine;
Outward-two the ring [2.2.1] heptan-2-base-(6-methoxyl group quinoxaline-2-yl)-amine;
Outward-and 2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy quinoxaline;
(two ring [2.2.2] sufferings-2-base oxygen base)-6,7-dimethoxy-quinoxaline;
In-2-(two rings [2.2.1] heptan-2-base oxygen base)-6,7-dimethoxy-quinoxaline;
Outward-and 2-(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline;
(two rings [2.2.1] heptan-5-alkene-2-base oxygen base)-6,7-dimethoxy quinoxaline;
2-cyclohexyloxy-6,7-dimethoxy quinoxaline;
2-encircles penta sulfenyl-6,7-dimethoxy quinoxaline;
6,7-dimethoxy-2-cyclopentyloxy-quinoxaline;
2-cyclopentyl methoxyl group-6,7-dimethoxy quinoxaline;
6,7-dimethoxy-2-tetrahydropyrans-4-oxygen base-quinoxaline;
Outward, outer-6,7-dimethoxy-2-(5,6-epoxy-two ring [2.2.1] heptane-2-base oxygen base) quinoxaline;
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base oxygen base)-hexahydrobenzoic acid;
6,7-dimethoxy-2-(4-methoxyl group-cyclohexyloxy)-quinoxaline;
(1R, 2R, 4S)-(+)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
(1S, 2S, 4R)-(-)-two the ring [2.2.1] heptan-2-base-(6,7-dimethoxy quinoxaline-2-yl)-amine;
(6,7-dimethoxy-quinoxaline-2-yl)-2-aza-bicyclo [2.2.2] octane-3-ketone;
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
Cis/trans-4-(6,7-dimethoxy-quinoxaline-2-base is amino)-hexahydrobenzoic acid;
Cis-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
Trans-4-(6,7-dimethoxy quinoxaline-2-base is amino)-hexahydrobenzoic acid methyl esters;
(6,7-dimethoxy-quinoxaline-2-yl)-cis/trans-(3-(R)-methylcyclohexyl) amine;
(6,7-dimethoxy-quinoxaline-2-yl)-trans-(3-(R)-methylcyclohexyl) amine;
(6,7-dimethoxy-quinoxaline-2-yl)-cis-(3-(R)-methylcyclohexyl) amine; Or
Cis/trans-4-(6,7-dimethoxy quinoxaline-2-base oxygen base)-hexahydrobenzoic acid methyl esters, or
Its N-oxide compound, its hydrate, its solvate, its prodrug or its pharmacologically acceptable salt.
47. a pharmaceutical composition, it comprises the compound of claim 1, or its pharmacologically acceptable salt and pharmaceutically acceptable carrier.
48. the method for a vitro inhibition PDGF tyrosine kinase activity, it comprises that the compound that makes claim 1 contacts with the composition that contains the PDGF Tyrosylprotein kinase.
49. the method for a vitro inhibition Lck tyrosine kinase activity, it comprises that the compound that makes claim 1 contacts with the composition that contains the Lck Tyrosylprotein kinase.
50. the compound or pharmaceutically acceptable salt thereof of claim 1 suppresses purposes in the medicine that patient's cell proliferation, differentiation or medium discharge in preparation, described patient suffers from the disease that is released to feature with this propagation and/or differentiation and/or medium.
51. the purposes of the compound or pharmaceutically acceptable salt thereof of claim 1 in the medicine of the symptom that the preparation treatment links to each other with the hyper-proliferative disease.
52. according to the purposes of claim 51, described symptom is a restenosis.
53. the purposes of the compound or pharmaceutically acceptable salt thereof of claim 1 in preparation treatment restenosis patient's medicine, the medicine that wherein contains the compound of claim 1 can suppress vascular smooth muscle cell proliferation and migration at predetermined position.
54. according to the purposes of claim 51, wherein said hyper-proliferative disease is positioned at when using angioplasty treatment atherosclerotic lesions the physical abuse place to arterial wall produced.
55. according to the purposes of claim 51, the medicine that wherein contains the compound or pharmaceutically acceptable salt thereof of claim 1 is used by the angioplasty air bag that is coated with by the saturated hydrophilic film of claim 1 compound.
56. according to the purposes of claim 51, the medicine that wherein contains the compound or pharmaceutically acceptable salt thereof of claim 1 is used by conduit, described conduit comprises the infusion groove that contains claim 1 compound solution.
57. the purposes of the compound or pharmaceutically acceptable salt thereof of claim 1 in the medicine of preparation treatment diseases associated with inflammation patient's inflammation.
58. according to the purposes of claim 52, wherein use by the method for the coating on the stent device, and comprise the compound of claim 1 in this coating with the medicine of the compound or pharmaceutically acceptable salt thereof that contains claim 1.
59. according to the purposes of claim 51, wherein the illness that links to each other with hyper-proliferative venereal disease shape is the cancer to the treatment sensitivity that suppresses the PDGF Tyrosylprotein kinase.
60. according to the purposes of claim 59, wherein cancer is the cancer of the brain, ovarian cancer, colorectal carcinoma, prostate cancer, lung cancer, Kaposi or malignant melanoma.
61. according to the purposes of claim 50, disease wherein is leukemia, cancer, glioblastoma, psoriasis, diseases associated with inflammation, osteopathy, fibrosis disease, sacroiliitis, pnemnofibrosis, kidney fibrosis, hepatic fibrosis sex change, atherosclerosis; Or the restenosis that takes place of coronary artery, femoral artery, Renal artery postangioplasty.
62. to the purposes in the medicine of diseases such as versus-host disease, asthma, inflammatory bowel disease or pancreatitis, the medicine that wherein contains the compound or pharmaceutically acceptable salt thereof of claim 1 can suppress the Lck Tyrosylprotein kinase to the compound or pharmaceutically acceptable salt thereof of claim 1 preparation treatment rheumatoid arthritis, multiple sclerosis, systemic lupus erythematous, graft.
63. the angioplasty balloon of the hydrophilic film dressing of the compound of using claim 1 saturated.
64. comprise the conduit of infusion chamber, the solution of the compound of claim 1 is contained in wherein said infusion chamber.
CNA2008101005365A 1997-05-28 1998-05-28 Quinoline and quinoxaline compounds which inhibit platelet-derived growth factor and/or p561lck tyrosine kinases Pending CN101293869A (en)

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