CN101289246B - 果胶酶在抑制藻华中的应用及方法 - Google Patents

果胶酶在抑制藻华中的应用及方法 Download PDF

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CN101289246B
CN101289246B CN2008100584178A CN200810058417A CN101289246B CN 101289246 B CN101289246 B CN 101289246B CN 2008100584178 A CN2008100584178 A CN 2008100584178A CN 200810058417 A CN200810058417 A CN 200810058417A CN 101289246 B CN101289246 B CN 101289246B
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CN101289246A (zh
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赖泳红
崔晓龙
文孟良
王海玉
李一青
赵江源
彭谦
李欣毅
葛娟
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Yunnan University YNU
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Abstract

果胶酶在抑制藻华中的应用及方法，是先将果胶酶粉加水配制成浓度在200U/ml以上、pH值为4.5-6.5的果胶酶溶液，再将果胶酶溶液加入藻液中。本发明可使蓝藻在短时间内丧失光合色素功能，从而使蓝藻水华得到控制。其治理藻华的成本低，还可避免对水体的二次污染，安全可靠。本发明较其它微生物法可控性好，能在较短时间内控制住藻华。

Description

果胶酶在抑制藻华中的应用及方法
技术领域
本发明涉及果胶酶的新用途，用于治理蓝藻水华的用途及其方法。
背景技术
果胶酶普遍应用于食品、饲料、纺织、医药工业及污水处理等领域，至今未见用于环境藻华治理方面的报道。蓝藻水华污染是全球环境治理的一个难题。目前应用生物技术治理蓝藻水华通常采用微生物方法，它较物理治理方法成本低，也避免了化学方法的二次污染，成为研究的热点，但是由于微生物在水环境中难定量及可控性差，故实际应用效果并不令人满意。
发明内容
本发明目的在于解决现有技术的不足而提供一种果胶酶的新用途以及用果胶酶抑制蓝藻水华的方法。
本发明的目的是通过如下技术方案实现的。
果胶酶在抑制藻华中的应用。
应用果胶酶抑制藻华的方法，是先将果胶酶粉加水配制成浓度在200U/ml以上、pH值为4.5-6.5的果胶酶溶液，再将果胶酶溶液加入藻液中。
藻液的藻浓度为107个/ml。
蓝藻细胞外有一层能抵御外界恶劣环境的胶质鞘，由果胶酸(pectic acid)和粘多糖(mucopolysaccharide)组成。溶藻菌或溶藻物质要侵入藻细胞，对藻细胞形成攻击，最先需要透过或破坏掉藻体表面的胶质鞘屏障。而果胶酶就具有水解胶质鞘的作用。本发明利用果胶酶能够水解胶质鞘这一特性来处理蓝藻藻华，通过酶解蓝藻细胞的胶质鞘保护层，进一步影响蓝藻细胞光合片层的功能，使蓝藻在短时间内丧失光合色素功能，从而使蓝藻水华得到控制。此外，由于果胶酶是一种生物酶，用量小，它较物理治理藻华方法成本低，也避免了化学方法的二次污染，安全可靠。本发明较其它微生物法可控性好，能在较短时间内控制住藻华。
具体实施方式
下面通过实施例进一步阐述本发明的内容。
实施例1：
在实验环境中，可按如下配比先配制藻液：
NaNO3 150mg，K2HPO4 4mg，MgSO4.7H2O 7.5mg，CaCl2.2H2O 3.6mg，Na2SiO3.9H2O 5.8mg，柠檬酸0.6mg，柠檬酸铁铵0.6mg，EDTA 0.1mg，Na2CO3 2mg，A5溶液0.1ml，蒸馏水99.9ml。(A5溶液：H3BO3 286mg，MnCl2.4H2O 181mg，ZnSO4.7H2O 22mg，CuSO4.5H2O 7.9mg，Na2MoO4.2H2O 3.9mg，蒸馏水100mL)。将藻液培养9-15天。藻浓度为107个/ml，叶绿素a浓度(OD值)为0.216。
先将果胶酶粉加水配制成浓度在200U/ml、pH值为4.5的果胶酶溶液，再将果胶酶溶液加入藻浓度为107个/ml的藻液中，果胶酶粉可选用美国WOLSEN公司生产的、25g包装、酶活20U/mg的果胶酶粉。
将加入了果胶酶溶液的藻液置于温度25-35℃，光照强度为2000-3000Lx的条件下观察果胶酶控藻情况。48hr后，观察到该酶液对蓝藻液叶绿素a的去除率可达90％以上。在添加果胶酶液3天后，藻液叶绿素浓度从0.216下降到0.005，肉眼观察不到叶绿素存在。与未加果胶酶的藻液对比，未加果胶酶的藻液叶绿素含量为0.216(OD值)，液体呈明显的深绿色。
实施例2：先将果胶酶粉加水配制成浓度在250U/ml、pH值为5的果胶酶溶液，再将果胶酶溶液加入藻浓度为107个/ml的藻液中。
实施例3：先将果胶酶粉加水配制成浓度在380U/ml、pH值为6.5的果胶酶溶液，再将果胶酶溶液加入藻浓度为107个/ml的藻液中。
实施例4：先将果胶酶粉加水加水配制成浓度在500U/ml、pH值为6的果胶酶溶液，再将果胶酶溶液加入藻浓度为107个/ml的藻液中。
实施例5：先将果胶酶粉配制成浓度在700U/ml、pH值为5.5的果胶酶溶液，再将果胶酶溶液加入藻浓度为107个/ml的藻液中。

Claims (3)

1.果胶酶在抑制藻华中的应用。
2.应用果胶酶抑制藻华的方法，其特征在于，先将果胶酶粉加水配制成浓度在200U/ml以上、pH值为4.5-6.5的果胶酶溶液，再将果胶酶溶液加入藻液中。
3.根据权利要求2所述的应用果胶酶抑制藻华的方法，其特征在于，藻液的藻浓度为107个/ml。
CN2008100584178A 2008-05-20 2008-05-20 果胶酶在抑制藻华中的应用及方法 Expired - Fee Related CN101289246B (zh)

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CN104944536A (zh) * 2015-06-03 2015-09-30 山东理工大学 一种抑制小球藻生长的方法
CN104944535A (zh) * 2015-06-03 2015-09-30 山东理工大学 抑制小球藻生长的一种方法
CN105062946A (zh) * 2015-07-27 2015-11-18 山东理工大学 一种促进小球藻生长的方法
CN105217801B (zh) * 2015-09-11 2018-04-17 中国环境科学研究院 环保酵素在防治湖泊藻类水华危害方面的应用
CN105200019B (zh) * 2015-09-11 2019-01-01 中国环境科学研究院 环保酵素和藻华抑制剂的制备方法
CN105145676B (zh) * 2015-09-11 2018-08-28 中国环境科学研究院 藻华抑制剂
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