CN101288422B - Aminopolysaccharides and/or oligosacchride preservative and preparation thereof - Google Patents

Aminopolysaccharides and/or oligosacchride preservative and preparation thereof Download PDF

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Publication number
CN101288422B
CN101288422B CN2008101148195A CN200810114819A CN101288422B CN 101288422 B CN101288422 B CN 101288422B CN 2008101148195 A CN2008101148195 A CN 2008101148195A CN 200810114819 A CN200810114819 A CN 200810114819A CN 101288422 B CN101288422 B CN 101288422B
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centipoises
glycosaminoglycan
antistaling agent
fruit
agent
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CN2008101148195A
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CN101288422A (en
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田世平
孟祥红
徐勇
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Institute of Botany of CAS
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Institute of Botany of CAS
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Abstract

The invention discloses an aminopolysaccharide and/or oligosaccharide preservative and a preparation method thereof. The method adopts three kinds of aminopolysaccharide and/or oligosaccharide substances of which the viscosities are respectively 5centipoises, 15 centipoises and 50 centipoises are compounded to serve as the effective components of the aminopolysaccharide and/or oligosaccharide preservative. The aminopolysaccharide and/or oligosaccharide preservative have/has a degree of deacetylation ranging from 80 percent to 85 percent. The aminopolysaccharide and/or oligosaccharide compounds with viscosities of respectively 5 centipoises, 15 centipoises and 50 centipoises are compounded in the weight ratio ranging from (3 to 1 to 1) to (7 to 2 to 1) so as to prepare the preservative applied to the spraying before harvest, and also can be compounded in a weight ratio of 1 to (ranging from 1 to 2) to 2 so as to prepare the immersion capsule preservative after being harvest.

Description

A kind of glycosaminoglycan and/or oligosaccharides antistaling agent and preparation method thereof
Technical field
The present invention relates to a kind of glycosaminoglycan and/or oligosaccharides antistaling agent and preparation method thereof.
Background technology
The grape fruit skin is thin, pulp contains saccharic acid amount height, and it is easily broken and suffer courses of infection to adopt the back, causes fruit rot; Adopt back fruit respiratory metabolism in addition, ethylene metabolism is vigorous, causes the weightless and quality of fruit to descend, therefore how to prevent and treat and adopt the back courses of infection and keep the key technology that fruit quality is the grape preservation and freshness.Present domestic patented technology relates generally to and adopts SO 2And the control of various slowly-releasings packing adopts the generation of the various diseases in back, utilizes low temperature, controlled atmosphere or CO 2Absorbent reduces the fruit weight-loss ratio, keeps fruit quality.But because SO 2Not only fruit self is damaged quality and the local flavor that influences fruit, simultaneously it residually has destruction to the human immune system, and the crisis consumer's is healthy, so SO 2Application more and more be restricted, developed countries such as America and Europe have banned use of, the at present domestic preservation technique that still lacks the grape of " green " safety.
Glycosaminoglycan is through extraction, processing and preparing from shellfish shells such as shrimp crab, chemical name is β (1 → 4)-poly-2-amino deoxy glucose, this material has multifrequency natures such as biological safety, edible, antibiotic property, film forming and degradable, is widely used in fields such as chemical industry, medical treatment, food, light industry, environmental protection and agricultural.
Summary of the invention
The purpose of this invention is to provide a kind of glycosaminoglycan and/or oligosaccharides antistaling agent and preparation method thereof.
The preparation method of glycosaminoglycan provided by the present invention and/or oligosaccharides antistaling agent is to be that the glycosaminoglycan and/or the oligosaccharides of 5 centipoises, 15 centipoises and 50 centipoises carries out the active ingredient of the compound complex that obtains as glycosaminoglycan and/or oligosaccharides antistaling agent with three kinds of viscosity.
Wherein, the deacetylation of described glycosaminoglycan and/or oligosaccharides antistaling agent is 80%-85%.
Above-mentioned shitosan can be from crab shell, dried small shrimp, insect shell or cicada slough, extract to, also can be the preparation of microbial fermentation, culture plant cell.
Spray before glycosaminoglycan of the present invention and/or oligosaccharides antistaling agent branch are adopted and use antistaling agent and adopt the back and soak coating antistaling agent two classes, with before being applied to grape fruit respectively and adopting and after adopting.
Spray before described the adopting use antistaling agent be with the glycosaminoglycan of described 5 centipoises, described 15 centipoises and described 50 centipoises and/or oligosaccharides according to (3-7): (1-2): 1 weight ratio is composite, and the compound that obtains is as spraying the active ingredient of using antistaling agent before adopting.
Described adopt the back soak the coating antistaling agent be with the glycosaminoglycan of described 5 centipoises, described 15 centipoises and described 50 centipoises and/or oligosaccharides compound according to 1: (1-2): 2 weight ratio is composite, and the mixture that obtains soaks the active ingredient of coating antistaling agent as adopting the back.
Wherein, before described the adopting of preparation, spray when using antistaling agent, described active ingredient is dissolved in acetate, lactic acid or the oxalic acid aqueous solution, the final concentration of described active ingredient is (200-1000) mg/L, and the organic acid concentration of volume percent is 0.05%-0.01% in described acetate, lactic acid or the oxalic acid aqueous solution; When after preparing described adopting, soaking the coating antistaling agent, described active ingredient is dissolved in acetate, lactic acid or the oxalic acid aqueous solution, the final concentration of described active ingredient is (2000-5000) mg/L, and the organic acid concentration of volume percent is 0.1%-0.2% in described acetate, lactic acid or the oxalic acid aqueous solution.Described adopting in the back immersion coating antistaling agent can add carboxymethyl cellulose or sodium alginate; The mass percent concentration of described carboxymethyl cellulose or sodium alginate is 0-0.1% (w/v).
To use antistaling agent and the described back of adopting is soaked the coating antistaling agent better result of use can be arranged in order making to spray before described the adopting, to spray before described the adopting and use antistaling agent and the described back of adopting is soaked in the coating antistaling agent and all also can be added emulsifying agent; Described emulsifying agent is Tween-80 or glycerine; The concentration of volume percent of described emulsifying agent is preferably 0.01-0.5%.And spray the application antistaling agent before described the adopting and also can add borate or calcium salt with adopting in the back immersion coating antistaling agent; The mass percent concentration of described borate or calcium salt is preferably 0.01-0.5% (w/v).
Wherein, spraying before of the present invention the adopting and using antistaling agent is 5-7 with the pH value of adopting back immersion coating antistaling agent, and the pH value of described antistaling agent can be regulated with inorganic acid, organic bronsted lowry acids and bases bronsted lowry; Example hydrochloric acid, sulfuric acid, phosphoric acid; Acetic acid, citric acid, lactic acid; NaOH, potassium hydroxide or sodium acid carbonate etc.
Belong to protection scope of the present invention with glycosaminoglycan and/or the oligosaccharides antistaling agent for preparing the method preparation of glycosaminoglycan and/or oligosaccharides antistaling agent of the present invention.
Active ingredient in glycosaminoglycan of the present invention and/or the oligosaccharides antistaling agent is to derive from the glycosaminoglycan compound that degradation of chitosan obtains.But the glycosaminoglycan coating can reduce fruit storage process percentage of water loss to a certain extent on fruit, keep the hardness of fruit; Reduce respiration of fruits speed and ethene growing amount, delay respiratory climacteric and ethene and discharge the peak; Reduce membrane permeability and oxygen production, the delayed fruit aging.Glycosaminoglycan and/or oligosaccharides have the antibiotic property of wide spectrum as a kind of material of polycation simultaneously, and the growth of adopting the back rotten fungus before fruit is adopted has significant inhibitory effect, and fruit disease generation and PD are had prevention effect.Glycosaminoglycan and/or oligosaccharides be as a kind of cause of disease exciton, adopt preceding, adopt post processing and can strengthen fruit self resistance related enzyme activity to a certain extent, induction of resistance material and self cyto-architectural variation improve the fruit disease resistance.Glycosaminoglycan of the present invention and/or oligosaccharides antistaling agent can be adopted the back fit applications in the preservation and freshness of grape fruit before adopting.
The specific embodiment
Embodiment 1, preparation glycosaminoglycan and/or oligosaccharides antistaling agent
It is composite and to be dissolved in percentage by volume be in 0.05% the oxalic acid solution according to 3: 1: 1 mass ratio to get 5 centipoises, 15 centipoises and 50 centipoise glycosaminoglycans (available from Qingdao Honghai Biotechnology Co., Ltd.), the final concentration of glycosaminoglycan and/or oligosaccharides is 200mg/L, add Tween80 subsequently, its final concentration is 0.02ml/100ml's, in above-mentioned solution, add Boratex then, the final concentration of Boratex is 0.03g/100ml's, and it is 6.0 that hydrochloric acid is transferred pH, and this is for spraying the application antistaling agent before adopting.
It is composite and to be dissolved in percentage by volume be in 0.1% the lactic acid solution according to 1: 1: 2 mass ratio to get 5 centipoises, 15 centipoises and 50 centipoise glycosaminoglycans and/or oligosaccharides, the final concentration of glycosaminoglycan and/or oligosaccharides is 2000mg/L, add Tween80 subsequently, its final concentration is 0.02ml/100ml's, in above-mentioned solution, add Boratex then, making its final concentration is 0.01g/100ml, it is 6.0 that hydrochloric acid is transferred pH, add carboxymethyl cellulose at last, making its final concentration is 0.05g/100ml, and this soaks the coating antistaling agent for adopting the back.
Embodiment 2, preparation glycosaminoglycan and/or oligosaccharides antistaling agent
It is composite and be dissolved in the lactic acid solution of percentage by volume 0.05% according to 4: 1: 1 mass ratio to get 5 centipoises, 15 centipoises and 50 centipoise glycosaminoglycans and/or oligosaccharides (available from Qingdao Honghai Biotechnology Co., Ltd.), the final concentration of glycosaminoglycan and/or oligosaccharides is 500mg/L, add Tween80 subsequently, its final concentration is 0.02ml/100ml's, in above-mentioned solution, add Boratex then, the final concentration of Boratex is 0.1g/100ml, and it is 6.0 that hydrochloric acid is transferred pH, and this is for spraying the application antistaling agent before adopting.
It is composite and be dissolved in the acetic acid solution of percentage by volume 0.2% according to 1: 2: 2 mass ratio to get 5 centipoises, 15 centipoises and 50 centipoise glycosaminoglycans and/or oligosaccharides, the final concentration of glycosaminoglycan and/or oligosaccharides is 4000mg/L, add Tween80 subsequently, its final concentration is 0.3ml/100ml, in above-mentioned solution, add Boratex then, making its final concentration is 0.01g/100ml, it is 6.0 that hydrochloric acid is transferred pH, add carboxymethyl cellulose at last, making its final concentration is 0.03g/100ml, and this soaks the coating antistaling agent for adopting the back.
Embodiment 3, preparation glycosaminoglycan and/or oligosaccharides antistaling agent
It is composite and be dissolved in the acetic acid solution of percentage by volume 0.01% according to 7: 2: 1 mass ratio to get 5 centipoises, 15 centipoises and 50 centipoise glycosaminoglycans and/or oligosaccharides (available from Qingdao Honghai Biotechnology Co., Ltd.), the final concentration of glycosaminoglycan and/or oligosaccharides is 1000mg/L, add glycerine subsequently, making its final concentration is 0.02ml/100ml, in above-mentioned solution, add Boratex then, its final concentration is 0.2g/100ml, and it is 6.0 that hydrochloric acid is transferred pH, and this is for spraying the application antistaling agent before adopting.
It is composite and be dissolved in the acetic acid solution of percentage by volume 0.1% according to 1: 1: 2 mass ratio to get 5 centipoises, 15 centipoises and 50 centipoise glycosaminoglycans and/or oligosaccharides, the final concentration of glycosaminoglycan and/or oligosaccharides is 5000mg/L, add Tween80 subsequently, making its final concentration is 0.3ml/100ml, in above-mentioned solution, add Boratex then, making its final concentration is 0.01g/100ml, it is 6.0 that hydrochloric acid is transferred pH, add sodium alginate at last, making its final concentration is 0.02g/100ml, and this soaks the coating antistaling agent for adopting the back.
The preservativity of embodiment 4, glycosaminoglycan of the present invention and/or oligosaccharides antistaling agent
Preceding 10 days of grape harvest is earlier used antistaling agent and is evenly sprayed " capital show " uva string with spraying before the adopting of preparation, and sprays the grape cluster of using antistaling agent before adopting and immerses and adopted back immersion coating antistaling agent 2 minutes spraying the same day after harvesting, and 25 ℃ are dried naturally.The uva string of dry tack free film forming is deposited in 1 ℃ with the polybag packing of semi-transparent gas, and humidity is 95%, and storage time is 3 months, plucks the back and directly deposits in 1 ℃ without the grape of any processing, and humidity is 95%, preserves 3 months, in contrast.
Soluble solid content, titratable acid content, weight-loss ratio, edible rate and rotting rate to grape after 3 months are measured and are estimated.
Soluble solid content adopts refractometer to measure, and represents with a ° Brix.Titratable acid content adopts general titration, represents with the mmol/100g fresh fruit.The fruit weight percent change was represented before and after weight-loss ratio adopted storage.
Edible rate adopts the trial test method to carry out statistical analysis.At first adopt triangle test to select 12 subjective appreciation persons, and to carry out 3 time with like product be one hour training.Each processing provides 360 grape fruits for evaluation.Edible fruit refers to that mode of appearance is normal, it is rotten not have, juice is abundant, the fruit free from extraneous odour; Not edible fruit refers to that fruit has putrefactive phenomenon or fruit that peculiar smell is arranged.The edible rate of fruit (%)=(edible fruit quantity/fruit total quantity) * 100.
Rotting rate (%) adopts statistical analysis technique.The healthy fruit of 1=; 2=has a bacterial plaque, and diameter is less than 3mm; Bacterial plaque of 3=, diameter is less than 10mm; Several bacterial plaques of 4=or fruit surface are lower than 25% to be infected; The 5=fruit surface surpasses 26% to be infected, and spore produces again.Rotting rate is calculated as follows: rotting rate (%)=100* (N1*1+N2*2+N3*3+N4*4+N5*5)/[(N1+N2+N3+N4+N5) * 5], N refers to that 1-5 refers to appropriate level fruit quantity.
3 months effect of table 1. " capital show " grape storage
Above-mentioned experimental result shows, carries out fresh-keeping grape with the method for embodiment 1,2 or 3, and after 3 months, its commodity has no significant change, rotting rate all<10%, weight-loss ratio<equal 5%.

Claims (7)

1. the preparation method of a glycosaminoglycan antistaling agent is to be that the glycosaminoglycan of 5 centipoises, 15 centipoises and 50 centipoises carries out the active ingredient of the compound complex that obtains as the glycosaminoglycan antistaling agent with three kinds of viscosity;
The glycosaminoglycan of described 5 centipoises, 15 centipoises and 50 centipoises is compound according to the weight ratio of following a or b:
The glycosaminoglycan of a, described 5 centipoises, 15 centipoises and 50 centipoises is according to (3-7): (1-2): 1 weight ratio is compound;
The glycosaminoglycan of b, described 5 centipoises, 15 centipoises and 50 centipoises is according to 1: (1-2): 2 weight ratio is compound.
2. the preparation method of glycosaminoglycan antistaling agent according to claim 1, it is characterized in that: described active ingredient is dissolved in acetate, lactic acid or the oxalic acid aqueous solution, the final concentration of described active ingredient is (200-1000) mg/L, and the concentration of volume percent of acetate, lactic acid or oxalic acid is 0.05%-0.01% in described acetate, lactic acid or the oxalic acid aqueous solution.
3. the preparation method of glycosaminoglycan antistaling agent according to claim 1 is characterized in that: described active ingredient is dissolved in acetate, lactic acid or the oxalic acid aqueous solution, and the final concentration of described active ingredient is (2000-5000) g/100ml; The concentration of volume percent of acetate, lactic acid or oxalic acid is 0.1%-0.2% in described acetate, lactic acid or the oxalic acid aqueous solution.
4. the preparation method of glycosaminoglycan antistaling agent according to claim 3 is characterized in that: also add carboxymethyl cellulose or sodium alginate in the described antistaling agent; Described carboxymethyl cellulose or the sodium alginate mass percent concentration in described antistaling agent is 0-0.1%.
5. according to the preparation method of claim 2 or 4 described glycosaminoglycan antistaling agents, it is characterized in that: also add emulsifying agent in the described antistaling agent; Described emulsifying agent is Tween-80 or glycerine; The concentration of volume percent of described emulsifying agent is 0.01-0.5%; The pH value of described antistaling agent is 5-7.
6. the preparation method of glycosaminoglycan antistaling agent according to claim 5 is characterized in that: also add borate or calcium salt in the described antistaling agent; Described borate or the calcium salt mass percent concentration in described antistaling agent is 0.01-0.5%.
7. the glycosaminoglycan antistaling agent of arbitrary described method preparation in the claim 1 to 6.
CN2008101148195A 2008-06-12 2008-06-12 Aminopolysaccharides and/or oligosacchride preservative and preparation thereof Expired - Fee Related CN101288422B (en)

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CN101433236B (en) * 2008-12-12 2013-08-28 青岛安芙兰芳香制品有限公司 Amino-oligosacchride composite antistaling agent and preparation method thereof
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