CN101270343A - High-quality ramie degumming bacterium and ramie degumming method - Google Patents
High-quality ramie degumming bacterium and ramie degumming method Download PDFInfo
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- CN101270343A CN101270343A CNA2008100472204A CN200810047220A CN101270343A CN 101270343 A CN101270343 A CN 101270343A CN A2008100472204 A CNA2008100472204 A CN A2008100472204A CN 200810047220 A CN200810047220 A CN 200810047220A CN 101270343 A CN101270343 A CN 101270343A
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Abstract
The invention relates to a ramie degumming bacterium, in particular to a high quality degumming bacterium acquired through irradiation with a plasma sprayer, and a method for degumming ramie by the degumming bacterium. A hoar bacterium or a golden yellow bacterium is got by irradiating and mutagenizing the degumming bacterium at ordinary pressure with the low-temperature plasma sprayer. The degumming method comprises the steps of A. irradiating and mutagenizing the degumming bacterium under ordinary pressure with the low-temperature plasma sprayer to get an improved hoar bacterium or a golden yellow bacterium; B. dissolving the modified hoar bacterium or the golden yellow bacterium, or the mixture of the hoar bacterium and the golden yellow bacterium with ramie in water to be cultured at the temperature of 20 DEG C to 30 DEG C for 2 to 5 hours. The degumming capability of the ramie degumming bacterium is qualitatively improved without damage to fibrilia. Impurities are completely eliminated through degumming, and the degumming speed is improved at least doubles.
Description
Technical field
The present invention relates to a kind of ramie degumming bacterium, specifically a kind of high-quality ramie degumming bacterium that obtains by plasma torch irradiation, and utilize this ramie degumming bacterium to carry out the method for China grass degumming.
Background technology
Vegetable fibre is the reproducible resource of nature, is the first-selected strongthener of cheap matrix material.In vegetable fibre, the content of cellulose height of ramee, intensity height belong to high performance natural plant fibre.China is the main place of production of ramie, accounts for more than 90% of world wide production, and ramie is in great demand all over the world as senior textile raw material.Greatly develop the ramie industry, immeasurable effect will be arranged the problem that increases income, solution surplus rural labor force problem, the nervous problem of solution industrial raw materials that solves China peasant.
Before extracting ramee, the degumming problem that the difficult point problem is exactly a ramie that people at first will solve.The normal chemical process that adopts the alkali lye kiering of coming unstuck at present, this method can cause to a certain degree damage to fiber, and kiering waste liquid alkalinity is many, and the sewage disposal difficulty is bigger.Consider that from environmental angle enzyme process and microbial method come unstuck and be considered to extremely rising method.Enzyme comes unstuck to have and can improve the quality of doing fiber crops, reduces remarkable advantage such as environmental pollution significantly.And in enzyme process, producing degummase by microbial method is a kind of enzyme producing method of generally adopting application.Therefore study microbial method, become one of key problem of enzyme process and microbial method by the ability that improves the production by biological degummase.
At present, people have separated, screened some the bacterial classification of the ability of coming unstuck, and has obtained some gratifying progress on the China grass degumming problem.Bacterial classification with the ability of coming unstuck has been arranged, breeding time of the bacterial classification of how accelerating aborning to come unstuck or improve the key that amount that the bacterial classification that comes unstuck produces degummase just becomes microbial degumming or enzymatic degumming method.Beyond any doubt, the speed that goes down to posterity of bacterial classification and the ability of coming unstuck are by its gene decision.Therefore how existing bacterial classification is improved, promptly how the mutagenesis bacterial classification, filter out the key issue that the microbial strains that helps coming unstuck just becomes following ramie industrial chain.The mutafacient system of microbial strains has chemical method, γ or ultraviolet mutagenesis method etc. at present, but studies show that the bacterial classification after these methods are handled for several times can produce resistance, and the performance of mutagenesis bacterial classification also is difficult to satisfy need of production practice repeatedly again.The eighties, China scientist is applied to ionic fluid in the work of biological germplasm asset creation earlier, has obtained some gratifying achievements.As the higher class of mutagenesis capability improving that common mutagenic compound can't be able to be realized with the mutafacient system of ionic fluid, the achievement of Vc generation bacterium that obtains with the ion beam mutagenesis method just has a net increase of 5,000 ten thousand yuan profit for enterprise every year.These achievements show that ion beam mutagenesis is very different with other mutafacient system, and ion has played the effect of a key in mutagenesis.General ion beam mutagenesis all is to carry out in a vacuum, is difficult to active somatic cell is carried out mutagenesis, seeks therefore that ion beam mutagenesis also is an important research direction under the normal pressure.At present, there is a kind of atmospheric plasma to use in the plasma physics, is mainly used in sterilization and fabric modifying field, do not see as yet and use atmospheric plasma to carry out the work of biological induced-mutation.Because contain low energy ion in the atmospheric plasma, atmospheric plasma also should be very different with electronics mutagenesis, visible light mutagenesis, ultraviolet and X ray mutagenesis.If can utilize ionic fluid and atmospheric plasma to carry out the mutagenesis of ramie degumming bacterium, suitable degumming bacterium might selected.
At present the subject matter of China grass degumming be come unstuck aborning that the speed of coming unstuck of bacterial classification is slow, degumming effect also a little less than.The wild-type strain kind of finding of coming unstuck is more at present, for example there is the mixed bacterium of bibliographical information black mold, genus bacillus, the spherical bacterium of chain to have the effect of coming unstuck (Hu Yansu of Donghua University etc.), a lot of reports are all introduced wild genus bacillus (as subtilis, end shape genus bacillus etc.) and are had the effect of coming unstuck, also there is report to introduce some bacterium and fungi also has the ability of coming unstuck (Chaudhury S D), but from the result of coming unstuck see degumming rate basically best be 40-60%, what have also has destruction to flaxen fiber.The biological characteristics difference of these bacterial classifications is bigger, but the general character of these bacterial classifications or mixed strains is that the characteristic of coming unstuck is all arranged.The mutafacient system of bacterial classification and strain improvement are key problems.Therefore the degumming bacterium of inventing a kind of modification will help to improve degumming quality, accelerate the speed of coming unstuck.Current biological induced-mutation improved system or device have a variety of, and these equipment are that a lot of contributions have been made in the establishment of biological species matter resource.But do not see the research of using plasma torch living body biological to be carried out mutagenesis both at home and abroad as yet.
The content of invention
Purpose of the present invention is exactly the defective that the speed of coming unstuck is slow, degumming rate is low at the existing bacterial classification that comes unstuck, a kind of high-quality ramie degumming bacterium that obtains by plasma torch irradiation is provided, and utilize this ramie degumming bacterium to carry out the method for China grass degumming, it can improve the degumming quality of ramie, accelerates the speed of coming unstuck.
Technical scheme of the present invention be achieved in that it be with ramie degumming bacterium under normal pressure, carry out a kind of greyish white bacterium or the golden yellow bacterium that irradiation mutagenesis obtains by the low-temperature plasma spray gun device.
The micrococci of wherein said ramie degumming bacterium for from the soil of plantation ramie, extracting.
Wherein said low-temperature plasma spray gun device comprises a grounding device, is provided with a silica tube in the middle of the grounding device, forms plasma slab between silica tube and the grounding device, and high voltage electrode is housed in the silica tube, and the upper end of silica tube is connected with inlet system.
Degumming method of the present invention may further comprise the steps:
A, with ramie degumming bacterium under normal pressure, carry out greyish white bacterium or the golden yellow bacterium that irradiation mutagenesis obtains a kind of improvement by the low-temperature plasma spray gun device;
B, the greyish white bacterium of modification or golden yellow bacterium or its mixed bacterium and ramie is soluble in water together carries out incubated at room temperature, and culture temperature is 20-30 ℃, and incubation time is 2-5 hour.
The micrococci of wherein said ramie degumming bacterium for from the soil of plantation ramie, extracting.
Wherein said low-temperature plasma spray gun device comprises a grounding device, is provided with a silica tube in the middle of the grounding device, forms plasma slab between silica tube and the grounding device, and high voltage electrode is housed in the silica tube, and the upper end of silica tube is connected with inlet system.
The ability of coming unstuck of ramie degumming bacterium of the present invention has the raising of matter, and to the flaxen fiber not damaged, rear impurity is almost all taken off through coming unstuck; The speed of coming unstuck doubles at least, and adopting bottle level of shaking, dead-beat to cultivate is 2 days at present the soonest.
Ultimate principle of the present invention is to use plasma torch technology ramie degumming bacterium to carry out irradiation, thereby causes mutagenesis, has obtained the new strong bacterial classification of the ability of coming unstuck.Irradiation system is by power-supply system 1, inlet system, and 2,3, discharge system, 4, the plasma torch system, 5, the irradiation target position constitutes.Total system is with on the cover cover, and pre-irradiation is existing to carrying out ultraviolet disinfection in this cover, to guarantee the gnotobasis of pre-irradiation system.The plasma flame of plasma torch ejection acts on organism, and then causes mutagenesis.Irradiation carries out at normal temperatures and pressures, so organism can be by irradiation under condition of living organism, and can be with water.
Power-supply system is to adopt the high voltage pulse technology, and maximum output voltage is more than the 25kV (zero load), more than the maximum current 600mA, more than the maximum 600W of rated output.
Inlet system is to have work carrier gas (generally being to adopt Ar gas) can sneak into other any reactant gases, is made of gas cylinder, gas pressure reducer, gas meter.When gas in cylindrical between electrode and the round shape outer electrode by the time obtain breakdown potential from forming cold plasma, this cold plasma outwards sprays by spout under gaseous tension.Gas both can be pure carrier gas, also can be mixed gas.All kinds of gas flow rates are generally 20 liters/minute, and its concrete scope is decided on job requirement.
The plasma torch system is made of inner high voltage electrode, round shape outer electrode and silica tube medium etc.The length of ejection plasma body line is decided by discharge power, discharge frequency and gas flow.The concrete size of spray gun is decided by actual needs.
Description of drawings
Fig. 1 is the synoptic diagram of whole irradiance method.
Fig. 2 is a low temperature normal atmosphere plasma gun system synoptic diagram
The 1st, power-supply system, the 2nd, inlet system, the 3rd, discharge system, the 4th, plasma slab, the 5th, target position, 6 tap into the gas system, and the 7th, high voltage electrode, the 8th, silica tube, the 9th, plasma slab, 10 grounding device.
Embodiment
Below in conjunction with embodiment and accompanying drawing to the present invention is further described:
The present invention will the micrococci that extract carry out a kind of greyish white bacterium or the golden yellow bacterium that irradiation mutagenesis obtains by the low-temperature plasma spray gun device under normal pressure from the soil of plantation ramie, or its mixed bacterium.As shown in Figure 2, described low-temperature plasma spray gun device comprises a grounding device 10, is provided with a silica tube 8 in the middle of the grounding device 10, forms plasma slab 9 between silica tube and the grounding device, high voltage electrode 7 is housed in the silica tube, and the upper end of silica tube is connected with inlet system 6.
Degumming method of the present invention may further comprise the steps:
A, will the micrococci that extract carry out irradiation mutagenesis by the low-temperature plasma spray gun device under normal pressure from the soil of plantation ramie, the time is greyish white bacterium or the golden yellow bacterium that obtained a kind of improvement in 3 minutes;
B, the ramie degumming bacterium and the ramie of modification is soluble in water together carries out incubated at room temperature, and culture temperature is 20-30 ℃, and incubation time is 2-5 hour.
As shown in Figure 2, described low-temperature plasma spray gun device comprises a grounding device 10, is provided with a silica tube 8 in the middle of the grounding device 10, forms plasma slab 9 between silica tube and the grounding device, high voltage electrode 7 is housed in the silica tube, and the upper end of silica tube is connected with inlet system 6.
The micrococci that to extract from the soil of plantation ramie carries out greyish white bacterium or the golden yellow bacterium that irradiation mutagenesis obtains a kind of improvement by the low-temperature plasma spray gun device under normal pressure.As shown in Figure 2, described low-temperature plasma spray gun device is with embodiment 1.
Degumming method of the present invention may further comprise the steps:
A, will the micrococci that extract carry out irradiation mutagenesis by the low-temperature plasma spray gun device under normal pressure from the soil of plantation ramie, the time is 5 minutes, and only mutagenesis once obtains a kind of greyish white bacterium of improvement and the mixed bacterium of golden yellow bacterium;
B, the mixed bacterium and the ramie of modification is soluble in water together carries out incubated at room temperature, and culture temperature is 20-30 ℃, incubation time is 2-5 hour, comes unstuck then, and the ability of coming unstuck of ramie degumming bacterium has the raising of matter, the flaxen fiber not damaged, impurity is almost all taken off.
Will be from the soil of plantation ramie the micrococci that extract under normal pressure, undertaken by the low-temperature plasma spray gun device that irradiation mutagenesis obtains.As shown in Figure 2, described low-temperature plasma spray gun device is with embodiment 1.
Degumming method of the present invention may further comprise the steps:
A, will the micrococci that extract carry out irradiation mutagenesis by the low-temperature plasma spray gun device under normal pressure from the soil of plantation ramie, the time is 5 minutes, and only mutagenesis once obtains a kind of greyish white bacterium or golden yellow bacterium of improvement;
B, the greyish white bacterium that will improve or golden yellow bacterium and ramie are soluble in water together, and under 30 degree left and right sides room temperatures, 500 ml shake flask non-oscillatories are cultivated, and incubation time is 2-5 hour, comes unstuck then, and the speed of coming unstuck reached about 2 days.The flaxen fiber not damaged, impurity is almost all taken off.Experiment all keeps the strong ability of coming unstuck repeatedly, and all can come unstuck preferably under tap water and distilled water state.This speed of coming unstuck also is than faster in the field of coming unstuck.
Claims (6)
1, a kind of high-quality ramie degumming bacterium, it be with ramie degumming bacterium under normal pressure, carry out a kind of greyish white bacterium or the golden yellow bacterium that irradiation mutagenesis obtains by the low-temperature plasma spray gun device.
2, high-quality ramie degumming bacterium according to claim 1, the micrococci of wherein said ramie degumming bacterium for from the soil of plantation ramie, extracting.
3, high-quality ramie degumming bacterium according to claim 1, wherein said low-temperature plasma spray gun device comprises a grounding device, be provided with a silica tube in the middle of the grounding device, form plasma slab between silica tube and the grounding device, high voltage electrode is housed in the silica tube, and the upper end of silica tube is connected with inlet system.
4, the Degumming method of a kind of ramie, it may further comprise the steps:
A, with ramie degumming bacterium under normal pressure, carry out greyish white bacterium or the golden yellow bacterium that irradiation mutagenesis obtains a kind of improvement by the low-temperature plasma spray gun device;
B, the greyish white bacterium of modification or golden yellow bacterium or its mixed bacterium and ramie is soluble in water together carries out incubated at room temperature, and culture temperature is 20-30 ℃, and incubation time is 2-5 hour.
5, the Degumming method of a kind of ramie according to claim 4, the micrococci of wherein said ramie degumming bacterium for from the soil of plantation ramie, extracting.
6, the Degumming method of a kind of ramie according to claim 4, wherein said low-temperature plasma spray gun device comprises a grounding device, be provided with a silica tube in the middle of the grounding device, form plasma slab between silica tube and the grounding device, high voltage electrode is housed in the silica tube, and the upper end of silica tube is connected with inlet system.
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| CNA2008100472204A CN101270343A (en) | 2008-04-03 | 2008-04-03 | High-quality ramie degumming bacterium and ramie degumming method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805511A (en) * | 2015-05-14 | 2015-07-29 | 苏州大学 | Pectin removal method of ramie product |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805511A (en) * | 2015-05-14 | 2015-07-29 | 苏州大学 | Pectin removal method of ramie product |
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Open date: 20080924 |