CN101266247B - HIV antibody immune magnetic beads rapid detection reagent kit - Google Patents
HIV antibody immune magnetic beads rapid detection reagent kit Download PDFInfo
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- CN101266247B CN101266247B CN 200810052963 CN200810052963A CN101266247B CN 101266247 B CN101266247 B CN 101266247B CN 200810052963 CN200810052963 CN 200810052963 CN 200810052963 A CN200810052963 A CN 200810052963A CN 101266247 B CN101266247 B CN 101266247B
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Abstract
The invention relates to a quick detection kit for detecting human immunologic deficiency disease (HIV) using nm immunomagnetic beads, related to biology applied technique field. The detected sample is added on the sampling pad of the test paper from the sampling hole and the sample dissolves the mark beads and chromatography is performed on the NC membrane, and then the data on the test paper is detected using magnetic force detector in preset time and the result is decided based on the read data. The kit is used for screening or clinical diagnosis of the HIV infection with high sensitivity, specificity, quickspeed, convenience and suitable for detection on site and real time recording and storing the result.
Description
Technical field
The present invention relates to the biologic applications technical field, relates to the HIV antibody diagnosing reagent, particularly relates to a kind of immunochromatography fast diagnosis reagent with nano immune magnetic bead technology for detection HIV antibody.
Background technology
Aids (Acquired Immunodeficiency syndrome, AIDS) be called for short acquired immune deficiency syndrome (AIDS), to infect a kind of serious infectious disease that causes by human immunodeficiency virus (Human immunodeficiency virus, HIV).Since the U.S. diagnosed out the first AIDS patient in 1981, according to up-to-date WHO data assessment, to the end of the year 2007, the whole world was estimated to have 3,300 ten thousand patients infected hivs, and China has patients infected hiv about 700,000.
The route of transmission of AIDS virus mainly comprises blood propagation, property contact, bang and mother-to-baby transmission.Average out to 7～10 years in latent period in human body.HIV infection patient in latent period does not have obvious clinical symptoms, but has the infectiousness of height.HIV is detected to find the infection sources and cuts off propagation by way of being the anti-the most effective means of AIDS processed.The HIV immunology detection is effective and the most widely used method, the HIV Antibody screening be China legal one of four haematogenous examination projects.
The most frequently used reagent of HIV antibody test is HIV antibody ELISA immunoreagent (ELISA) and collaurum class reagent, adopt the third generation HIV antibody diagnosing reagent kit of dual-antigen sandwich method, sensitivity reaches more than 99%, and specificity is used widely at present greater than 97%.The basic characteristics of two class reagent are as follows: ELISA reagent needs special-purpose laboratory and professional to test, complicated operation, but sensitivity is higher; Collaurum class reagent is easy and simple to handle, quick, be suitable for Site Detection, but sensitivity is slightly low, and the result with the naked eye directly observes, and can produce error, also can not record.
The nano immune magnetic bead is the superparamagnetic nano particle that contains ferro element, and the outside is coating the polyethylene kind polymer substance, and can with carboxyl or amino, be used for and cross linking of protein molecule.Immunomagnetic beads all is applied in cell and large molecular separation, purifying and diagnosis.But on the immunochromatography diagnostic reagent, also rarely has utilization.U.S. Quantum Design company is up-to-date to have released the detector (MICT) of measuring micro-magnetisable material on the chromatographic test paper, can quantitatively detect the magnetisable material of trace (pg level), and the development that detects reagent for the nano immune magnetic bead provides may.
It is as follows that the nano immune magnetic bead detects the reagent ultimate principle: utilize a kind of antigen of immunomagnetic beads mark or antibody, at the coated corresponding pairing antigen of the NC of reagent film or antibody, during detection when containing corresponding specific antibody or antigen in the sample, the part formation compound that combines in mark magnetic bead particles and the sample, then chromatography on the NC film, coated antigen or antibody capture are gathered on the detection line again, measure the judgement that the content of catching magnetic bead on the detection line is realized the result by MICT.Agent is compared with collaurum examination class, the difference that immunomagnetic beads detects the reagent maximum is to change the mark collaurum into the mark magnetic bead, with MICT the magnetic bead content on the detection line is detected during testing result, rather than rely on naked eyes to go total judgement, make the result judge more accurate, sensitive, objective and be convenient to record and preserve.Utilize nano immune magnetic bead development HIV antibody test fast diagnosis reagent to there is no report.
Summary of the invention
The present invention provides a kind of highly sensitive, high specificity, detection HIV antibody nano immune magnetic bead quick detection kit technical scheme quick, easy and simple to handle, that be fit to Site Detection and real time record saving result is as follows:
A kind of human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit, it is characterized in that test strips is by overlapping the loading pad at plastic support board one end, the one end overlap joint of loading pad contains the nano immune magnetic bead pad of mark Human Immunodeficiency Virus antigen gp41, gp36, at the end overlap joint cellulose nitrate NC of magnetic bead pad film, the NC film is coated with gp41 detection line detection line gp36 detection line and the nature controlling line that is separated from each other, NC film other end overlap joint is inhaled the sample pad and is formed, and test strips is packed into to be consisted of in the plastics kit.
Described human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit, it is characterized in that described mark and being coated with antigen is recombinant expressed Human Immunodeficiency Virus antigen gp41, gp36, also can increase or replace with other specific antigen of HIV gp160, gp120, P24 and O hypotype gp41.
Described human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit is characterized in that the nano immune magnetic bead is the superparamagnetic latex particle of iron content, and diameter is 200 ± 50nm, and carboxyl is rich on the surface; The plastic support plate thickness is less than 0.3mm; The width of NC film is 35mm; The loading pad is glass fibre membrane or nonwoven fabrics, inhales the sample pad and is made of absorbent filter.
Described human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit, it is characterized in that, the method for coating of antigen is: the solution of respectively gp41, gp36 psma ligand being made 1mg/ml, 0.5mg/ml with 0.01M pH 7.2 phosphate buffers (PBS), rule respectively with the parameter of 1ul/cm in NC film bottom with spray film instrument, coated T1, T2 line are respectively applied to detect gp41, gp36 antibody; Be coated with simultaneously anti-HIV antibody on NC film top as the C line.Distance between each coated line is 5mm, after the line with the NC film drying room (temperature 20-25 ℃, humidity is less than 30%) dry 8-10 hour.
Described human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit, the labeling method that it is characterized in that antigen is: get the 10mg magnetic bead, with 50mM 2-(N-morpholine) ethyl sulfonic acid (MES, pH5.7) be mixed with 1% magnetic bead solution, add 0.5mg water-soluble carbodiimide (EDC), 37 ℃ of 10min, add 200ug HIV gp41, gp36 merges or hybrid antigen, hatches 2 hours for 37 ℃ again, constantly rock during this time, then adsorb magnetic bead with magnetic frame, buffer exchange to 0.01m PBS (pH7.2), is contained 0.5% bovine serum albumin(BSA) (BSA), 0.5% tween is finished mark.With working fluid magnetic bead is mixed with the solution of 0.5mg/ml again, presses 1ml solution and spread 22cm
2Ratio be layered on equably on glass fibre membrane or the nonwoven fabrics, put again drying room (temperature 20-25 ℃, humidity is less than 30%) dry 2-4 hour, make the nano immune magnetic pad.
Described human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit, it is characterized in that, the assembly method of kit is: in hothouse (temperature 20-25 ℃, humidity is less than 30%), get the plastic support board plate, paste at the middle part that coated NC film is placed on the plastic support board plate, paste at NC film T line one side overlap joint immunomagnetic beads pad (take immunomagnetic beads pad 1/3), paste loading pad (take immunomagnetic beads pad 1/5) at immunomagnetic beads pad opposite side overlap joint; Inhale sample pad (take inhale sample pad 1/10) at NC film C line one side overlap joint; Paste one deck marking film topmost, then will post the plastic support card with cutter and be cut into the wide test strips of 5mm, the plastic clip of then test paper that cuts being packed into is finished the assembling of kit.
Described human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit, it is characterized in that, detection method is: with tested serum or blood plasma balance to the greenhouse, kit is kept flat, add the 40ul test sample at the loading pad, and then adding 60-100ul sample diluting liquid (0.01M PBS), sample will dissolve collaurum and chromatography on the NC film, test data on the paper slip with magnetic force detector inspection (MICT) in 25-40 minute behind the application of sample, according to the data judging result who reads.If the data that read contain anti-HIV antibody greater than the standard value of setting in the interpret sample, if be lower than the standard value of setting, the negative or content of interpret sample is lower than the lowest detectable limit of kit.
Described human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit, it is characterized in that described kit 10 is by 20 consisting of at the bottom of lid 11, the box, 20 all is that employing is plastic injection-moulded integrally formed at the bottom of lid 11, the box, and 20 all is that elongate in shape and its following middle part that two upper angles are circular shape arranges the square depression that makes progress at the bottom of lid 11, the box;
The length of lid 11, wide length with reagent strip, wide being equipped with, lid 11 is plate shape, in the lid front, left side slot being set is well 13, be positioned at its square depression top arrange many long, short different reaction zone indicates salient rib 12, a projection 15 each side is set in the lid back, the left and right sides vertically respectively arranges two raised plate 14 below projection 15, two protruding pressing plates 19 vertically are set in well 13 left sides, the height of the aspect ratio raised plate 14 of projection pressing plate 19, a protruding pressing plate 18 respectively is set in inboard, square depression both sides, the height of raised plate 18 is identical with the height of protruding pressing plate 19, in protruding pressing plate 17 of the right side of left hand projection plate 14 horizontally set, edge and raised plate 14 in the lid back, projection pressing plate 17, projection pressing plate 18, the edge of projection pressing plate 19 arranges a plurality of projections 16;
20 length respectively will grow the wing 21 at the bottom of the box than the length both sides of lid 11 at the bottom of the box, 20 wide, height wide, high and lid 11 are equipped with at the bottom of the box, 20 is plate shape at the bottom of the box, salient rib 23, protruding diagonal rib 24, band groove salient rib 27, rib 28 are set on 20 plates at the bottom of the box, at salient rib 23, protruding diagonal rib 24, band groove salient rib 27, rib 28 protruding hole 25 is set, at the bottom of box 20 a protruding hole 22 each side is set
Position that two grooves 26 inhale sample pads 4 corresponding wells 13 with human immunodeficiency virus (HIV) the antibody nano immune magnetic bead quick detection reagent bar 4 that the cuts kit 10 of packing into vertically respectively is set between 20 left and right sides band groove salient rib 27 at the bottom of the box and rib 28, namely gets human immunodeficiency virus (HIV) antibody nano immune magnetic bead quick detection kit.
Beneficial effect of the present invention is as follows: develop a kind of HIV antibody nano immune magnetic bead quick detection kit.Compare with collaurum class reagent, inherited collaurum class reagent easy and simple to handle, detect fast and be suitable for the advantage such as Site Detection, but result's instrument interpretation, rather than with the naked eye directly observe, avoided personal error, it is more accurate that the result is judged, the result can real time record preserve simultaneously.The characteristics of ELISA reagent have been used for reference simultaneously with the instrument result of determination, the difference that makes naked eyes be difficult to distinguish can distinguish, simultaneously because the high sensitivity of MICT instrument so that the sensitivity of nano immune magnetic bead quick detection reagent improves, reaches the sensitivity of ELISA reagent.
Description of drawings:
Fig. 1 immunomagnetic beads reagent strip structural representation.
Fig. 2 immunomagnetic beads detects the lid front view of reagent plastic casing.
Fig. 3 immunomagnetic beads detects the lid rear view of reagent plastic casing.
Front view at the bottom of the box of Fig. 4 immunomagnetic beads detection reagent plastic casing.
The reference numeral explanation:
1: loading pad 2: the magnetic bead pad
3:NC film 4: inhale the sample pad
5:gp41 detection line (T1) 6:gp36 detection line (T2)
7: test paper nature controlling line (C) 8: plastic support board
9: test strips 10: kit;
11: lid 12: reaction zone indicates salient rib
13: well 14: raised plate
15: projection 16: projection
17: protruding pressing plate 18: protruding pressing plate
19: protruding pressing plate 20: at the bottom of the box
21: the wing 22 at the bottom of the box: protruding hole
23: salient rib 24: protruding diagonal rib
25: protruding hole 26: groove
27: band groove salient rib 28: rib
Embodiment
Embodiment 1: preparation human immunodeficiency virus (HIV) antibody nano immune magnetic bead quick detection reagent bar:
1 main material
1.1 recombinant antigen: HIV gp41, gp36: U.S. Biotech Atlantic Inc (BAI.) product is used for being coated with and mark of reagent; The nano immune magnetic bead: French AdemTech company product, diameter 200nm, the surface is carboxyl modified; NC film: Millipore company product; EDC, MES, BSA:Sigma company product.Other common agents is analytical reagent.
1.2 HIV antibody National reference (collaurum class): Nat'l Pharmaceutical ﹠ Biological Products Control Institute's inspection development.Comprise 20 parts of positive serums, 20 parts of negative serums, 3 parts of sensitivity serum, 1 part of precision serum.
1.3 contain chaff interference serum: comprise respectively 10 parts of common interference object height blood fat, haemolysis, jaundice serum, contain each 10 parts of infectious disease HAV, HBV, HIV, TP and HP Positive Seras, contain each 10 parts of different anti-coagulants heparin, EDTA and sodium citrate blood plasma, collect checking and preservation by our company in the relevant hospital of Efficiency in Buildings in Tianjin Area, above serum or blood plasma detect through two or more ELISA and are HIV negative antibody serum.
1.4 600 parts of clinical serum HIV negative serums are collected in the relevant hospital of Efficiency in Buildings in Tianjin Area by company, are outpatient service collection check serum, detect through two or more ELISA and are HIV negative antibody serum.20 parts of HIV positive serums are obtained in relevant AIDS section hospital by company, prove conclusively Positive Sera, deactivation into HIV.
2 methods
2.1 HIV antigenic mark: get the 10mg magnetic bead, be mixed with 1% nanometer magnetic bead solution with 50mM MES (pH5.7), add the water-soluble EDC of 0.5mg, 37 ℃ of 10min, add 200ug HIV gp41, gp36 merges or hybrid antigen, hatches 2 hours for 37 ℃ again, constantly rock during this time, then adsorb magnetic bead with magnetic frame, buffer exchange to 0.01m PBS (pH7.2), is contained 0.5%BSA (bovine serum albumin), 0.5% polysorbas20 is finished mark.Use again working fluid (20mM borate buffer solution, pH8.5 contains 1%BSA, 0.5% tween, 5% sucrose) to make the solution of 0.5mg/ml, press 1ml solution and spread 22cm
2Ratio be layered on equably on glass fibre membrane or the nonwoven fabrics, put again drying room (temperature 20-25 ℃, humidity is less than 30%) dry 2-4 hour, make the nano immune magnetic pad, drying for standby.
2.2 the antigen coated solution of respectively gp41, gp36 psma ligand being made 1mg/ml, 0.5mg/ml with 0.01M pH 7.2 phosphate buffers (PBS) of HIV, rule respectively with the parameter of 1ul/cm in NC film bottom with spray film instrument, coated T1, T2 line are respectively applied to detect gp41, gp36 antibody.Be coated with simultaneously anti-HIV antibody on NC film top as the C line.Distance between each coated line is 5mm, after the line with the NC film drying room (temperature 20-25 ℃, humidity is less than 30%) dry 8-10 hour, for subsequent use.
2.3 Fig. 1 immunomagnetic beads reagent strip structural representation, as shown in Figure 1, HIV antibody rapid diagnosis reagent bar 9 be assembled in the hothouse (temperature 20-25 ℃, humidity is less than 30%), get plastic support board 8, paste at the middle part that coated NC film is placed on plastic support board 8, pastes at NC film T line one side overlap joint immunomagnetic beads pad 2 (take immunomagnetic beads pad 1/3), pastes loading pad 1 (take immunomagnetic beads pad 1/5) at immunomagnetic beads pad 2 opposite sides overlap joint; Inhale sample pad 4 (take inhale sample pad 1/10) at NC film C line one side overlap joint; Paste one deck marking film topmost, then sign gp41 detection line (T1) 5, gp36 detection line (T2) 6, test paper A nature controlling line (C) 7 will post the plastic support card with cutter and be cut into the wide reagent strip of 5mm on the film, then the kit of the reagent strip that cuts being packed into, the assembling of finishing kit gets final product.
2.4 detection method with tested serum or blood plasma balance to the greenhouse, kit is kept flat, add the 40ul test sample by well at the loading pad, and then adding 60-100ul sample diluting liquid (0.01M PBS), sample will dissolve collaurum and chromatography on the NC film, test data on the paper slip with magnetic force detector inspection (MICT) in 25-40 minute behind the application of sample, according to the data judging result who reads.If the data that the T1 line reads are greater than the standard value of setting, contain anti-HIV1 antibody in the interpret sample, if the data that the T2 line reads are greater than the standard value of setting, contain anti-HIV2 antibody in the interpret sample, T1 or (with) T2 is greater than the standard of setting, the result is positive, if T1 and T2 are lower than the standard value of setting, the negative or content of interpret sample is lower than the lowest detectable limit of kit.The C line needs greater than the standard of setting as the Quality Control of product, if less than established standards, it is invalid to detect, and needs again to detect.
2.5 pattern detection is by detection method, to HIV antibody National reference (collaurum class), contains chaff interference serum and clinical serum detects with the reagent that assembles.
3 results
3.1 HIV antibody National reference (collaurum class) detects National reference serum dish is tested, national Specification is passed through in result and expection (table 1) in full accord.
This kit of table 1 is to the test result of HIV antibody National reference (collaurum class)
* detect by MICT Instrument measuring reading, set T1, T2 setting value=75.0
3.2 chaff interference serum test result shows that this test paper detects all negative to common interference object height blood fat, haemolysis, jaundice serum, all negative to containing infectious disease HAV, HBV, HIV, TP and the detection of HP Positive Sera, detect all negative to containing different anti-coagulants heparin, EDTA and sodium citrate blood plasma.Explanation does not have nonspecific reaction to above material.
3.3 the clinical sample test: the testing result to clinical serum sees Table 2:
Table 2: this reagent is to the testing result of clinical serum
Specificity=591/600=98.5%, sensitivity is=20/20=100%; Total coincidence rate=611/260=98.
Embodiment 2: preparation human immunodeficiency virus (HIV) antibody nano immune magnetic bead quick detection kit 10:
Fig. 2 immunomagnetic beads detects the lid front view of reagent plastic casing.Fig. 3 immunomagnetic beads detects the lid rear view of reagent plastic casing.Front view at the bottom of the box of Fig. 4 immunomagnetic beads detection reagent plastic casing.Such as Fig. 2, Fig. 3, shown in Figure 4, human immunodeficiency virus (HIV) antibody nano immune magnetic bead quick detection kit 10 be by at the bottom of lid 11, the box 20, human immunodeficiency virus (HIV) antibody nano immune magnetic bead quick detection reagent bar consists of, 20 all is that employing is plastic injection-moulded integrally formed at the bottom of lid 11, the box, and 20 all is that elongate in shape and its following middle part that two upper angles are circular shape arranges the square depression that makes progress at the bottom of lid 11, the box.
The length of lid 11, wide length with reagent strip, wide being equipped with, lid 11 is plate shape, in the lid front, left side slot being set is well 13, be positioned at its square depression top arrange many long, short different reaction zone indicates salient rib 12, a projection 15 each side is set in the lid back, the left and right sides vertically respectively arranges two raised plate 14 below projection 15, two protruding pressing plates 19 vertically are set in well 13 left sides, the height of the aspect ratio raised plate 14 of projection pressing plate 19, a protruding pressing plate 18 respectively is set in inboard, square depression both sides, the height of raised plate 18 is identical with the height of protruding pressing plate 19, in protruding pressing plate 17 of the right side of left hand projection plate 14 horizontally set, edge and raised plate 14 in the lid back, projection pressing plate 17, projection pressing plate 18, the edge of projection pressing plate 19 arranges a plurality of projections 16.
20 length respectively will grow the wing 21 at the bottom of the box than the length both sides of lid 11 at the bottom of the box, at the bottom of the box 20 wide, height is wide with lid 11, height is equipped with, 20 is plate shape at the bottom of the box, salient rib 23 is set on 20 plates at the bottom of the box, projection diagonal rib 24, band groove salient rib 27, rib 28, at salient rib 23, projection diagonal rib 24, band groove salient rib 27, protruding hole 25 is set on the rib 28, two grooves 26 vertically respectively are set with the position of the loading pad 1 corresponding well 13 of human immunodeficiency virus (HIV) the antibody nano immune magnetic bead quick detection reagent bar 9 that the cuts kit 10 of packing in 20 the protruding hole 22 that each side arranges at the bottom of the box between 20 left and right sides band groove salient rib 27 at the bottom of the box and rib 28, namely get human immunodeficiency virus (HIV) antibody nano immune magnetic bead quick detection kit.
Claims (3)
1. a human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit (10) is characterized in that the mark of test strips (9) is recombinant expressed Human Immunodeficiency Virus antigen gp41, gp36 with being coated with antigen; Test strips (9) is by overlapping loading pad (1) at plastic support board (8) one ends, the one end overlap joint of loading pad (1) contains the recombinant expressed Human Immunodeficiency Virus antigen gp41 of mark, the nano immune magnetic bead pad (2) of gp36, end overlap joint cellulose nitrate NC film (3) at magnetic bead pad (2), NC film (3) is coated with the gp41 detection line (5) that is separated from each other, gp36 detection line (6) and nature controlling line (7), NC film (3) other end overlap joint is inhaled sample pad (4) and is formed, and test strips (9) is packed in the kit (10);
(20) all are that employing is plastic injection-moulded integrally formed at the bottom of the lid (11) of described kit (10), the box, and (20) all are that elongate in shape and its following middle part that two upper angles are circular shape arranges the square depression that makes progress at the bottom of lid (11), the box;
The length of lid (11), wide length with reagent strip, wide being equipped with, lid (11) is plate shape, in the lid front, left side slot being set is well (13), be positioned at its square depression top arrange many long, short different reaction zone indicates salient rib (12), a projection (15) each side is set in the lid back, in the left and right sides, projection (15) below two raised plate (14) are set respectively vertically, in well (13) left side two the first protruding pressing plates (19) are set vertically, the height of the aspect ratio raised plate (14) of the first protruding pressing plate (19), a second protruding pressing plate (18) respectively is set in inboard, square depression both sides, the height of the second protruding pressing plate (18) is identical with the height of the first protruding pressing plate (19), in the 3rd protruding pressing plate (17) of right side horizontally set of left hand projection plate (14), edge and raised plate (14) in the lid back, the 3rd protruding pressing plate (17), the second protruding pressing plate (18), the edge of the first protruding pressing plate (19) arranges a plurality of edges projections (16);
The length of (20) respectively will grow the wing (21) at the bottom of the box than the length both sides of lid (11) at the bottom of the box, wide, the height wide, high and lid (11) of (20) is equipped with at the bottom of the box, (20) are plate shape at the bottom of the box, salient rib (23), protruding diagonal rib (24), band groove salient rib (27), rib (28) are set on (20) plate at the bottom of the box, at salient rib (23), protruding diagonal rib (24), band groove salient rib (27), rib (28) protruding hole (25) on the rib are set, (20) each side arranges a protruding hole (22) at the bottom of box;
Between the left and right sides of (20) at the bottom of box band groove salient rib (27) and rib (28), two grooves (26) are set respectively vertically, with the position of the corresponding well of loading pad (1) (13) of human immunodeficiency virus (HIV) the antibody nano immune magnetic bead quick detection reagent bar (9) that the cuts kit (10) of packing into, namely get human immunodeficiency virus (HIV) antibody immune magnetic beads rapid detection reagent kit;
The nano immune magnetic bead is the superparamagnetic latex particle of iron content, and diameter is 200 ± 50nm, and carboxyl is rich on the surface; The plastic support plate thickness is less than 0.3mm; The width of NC film is 35mm; The loading pad is glass fibre membrane or nonwoven fabrics, inhales the sample pad and is made of absorbent filter.
2. human immunodeficiency virus according to claim 1 (HIV) antibody immune magnetic beads rapid detection reagent kit (10) is characterized in that: described mark and coated other specific antigen of HIV chosen wantonly from gp160, gp120, P24 and O hypotype gp41 that also increases with antigen.
3. human immunodeficiency virus according to claim 1 (HIV) antibody immune magnetic beads rapid detection reagent kit (10) is characterized in that: described mark and coated other specific antigen of HIV chosen wantonly from gp160, gp120, P24 and O hypotype gp41 that replaces with antigen.
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| CN 200810052963 CN101266247B (en) | 2008-04-30 | 2008-04-30 | HIV antibody immune magnetic beads rapid detection reagent kit |
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| CN 200810052963 CN101266247B (en) | 2008-04-30 | 2008-04-30 | HIV antibody immune magnetic beads rapid detection reagent kit |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101762700A (en) * | 2009-06-24 | 2010-06-30 | 北京科美东雅生物技术有限公司 | Magnetic immuno-chromatographic test paper strip for quantitatively detecting carcinoembryonic antigen in blood and preparation method thereof |
| CN101750496A (en) * | 2009-10-16 | 2010-06-23 | 北京科美东雅生物技术有限公司 | Magnetic immunochromatographic test strip for detecting hepatitis B e antibody and preparation method thereof |
| CN103558381B (en) * | 2013-11-06 | 2016-05-11 | 昆明云大生物技术有限公司 | Detect immune chromatography test paper of mankind antibody of AIDS virus and preparation method thereof |
| CN111273004B (en) * | 2020-03-09 | 2024-01-19 | 北京华晟源医疗科技有限公司 | Reagent strip for detecting HIV (l+2) antibody in urine based on colloidal gold method and preparation method thereof |
| CN114966011B (en) * | 2022-02-23 | 2024-04-16 | 杭州协合医疗用品有限公司 | Test strip for detecting HIV-1 and HIV-2 antibodies in urine by colloidal gold method |
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| CN1975423A (en) * | 2006-09-06 | 2007-06-06 | 浙江清华长三角研究院 | Immuno magnetic bead and producing method, and method and test plate for detection |
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| CN1975423A (en) * | 2006-09-06 | 2007-06-06 | 浙江清华长三角研究院 | Immuno magnetic bead and producing method, and method and test plate for detection |
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