CN101265413B - Method for preparing biological diesel oil from immobilized lipase-porous membrane biological reactor - Google Patents
Method for preparing biological diesel oil from immobilized lipase-porous membrane biological reactor Download PDFInfo
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- CN101265413B CN101265413B CN2008100605494A CN200810060549A CN101265413B CN 101265413 B CN101265413 B CN 101265413B CN 2008100605494 A CN2008100605494 A CN 2008100605494A CN 200810060549 A CN200810060549 A CN 200810060549A CN 101265413 B CN101265413 B CN 101265413B
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- 239000012528 membrane Substances 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000002283 diesel fuel Substances 0.000 title description 15
- 239000004367 Lipase Substances 0.000 claims abstract description 32
- 102000004882 Lipase Human genes 0.000 claims abstract description 32
- 108090001060 Lipase Proteins 0.000 claims abstract description 32
- 235000019421 lipase Nutrition 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000004519 grease Substances 0.000 claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000003225 biodiesel Substances 0.000 claims abstract description 17
- 230000003197 catalytic effect Effects 0.000 claims abstract description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 45
- 239000000203 mixture Substances 0.000 claims description 22
- 239000003921 oil Substances 0.000 claims description 22
- 235000019198 oils Nutrition 0.000 claims description 22
- 239000002551 biofuel Substances 0.000 claims description 12
- 229910052799 carbon Inorganic materials 0.000 claims description 12
- 229920002492 poly(sulfone) Polymers 0.000 claims description 10
- 241001661345 Moesziomyces antarcticus Species 0.000 claims description 9
- 239000000835 fiber Substances 0.000 claims description 9
- 241000235403 Rhizomucor miehei Species 0.000 claims description 6
- 210000000582 semen Anatomy 0.000 claims description 6
- 239000000919 ceramic Substances 0.000 claims description 5
- 235000021323 fish oil Nutrition 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 4
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 241000269319 Squalius cephalus Species 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 239000003549 soybean oil Substances 0.000 claims description 4
- 235000012424 soybean oil Nutrition 0.000 claims description 4
- ZEMPKEQAKRGZGQ-AAKVHIHISA-N 2,3-bis[[(z)-12-hydroxyoctadec-9-enoyl]oxy]propyl (z)-12-hydroxyoctadec-9-enoate Chemical compound CCCCCCC(O)C\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CC(O)CCCCCC)COC(=O)CCCCCCC\C=C/CC(O)CCCCCC ZEMPKEQAKRGZGQ-AAKVHIHISA-N 0.000 claims description 3
- 241000228245 Aspergillus niger Species 0.000 claims description 3
- 241001453380 Burkholderia Species 0.000 claims description 3
- 241000195493 Cryptophyta Species 0.000 claims description 3
- 241000222175 Diutina rugosa Species 0.000 claims description 3
- 239000002033 PVDF binder Substances 0.000 claims description 3
- 235000019483 Peanut oil Nutrition 0.000 claims description 3
- 235000019484 Rapeseed oil Nutrition 0.000 claims description 3
- 241000223258 Thermomyces lanuginosus Species 0.000 claims description 3
- 239000008157 edible vegetable oil Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000000312 peanut oil Substances 0.000 claims description 3
- 229920002239 polyacrylonitrile Polymers 0.000 claims description 3
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 238000007670 refining Methods 0.000 claims description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract description 20
- 108090000790 Enzymes Proteins 0.000 abstract description 7
- 102000004190 Enzymes Human genes 0.000 abstract description 7
- 239000006227 byproduct Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000004140 cleaning Methods 0.000 abstract description 2
- 239000002904 solvent Substances 0.000 abstract description 2
- 238000012822 chemical development Methods 0.000 abstract 1
- 239000012510 hollow fiber Substances 0.000 abstract 1
- 238000003860 storage Methods 0.000 description 36
- 239000002994 raw material Substances 0.000 description 16
- 108010093096 Immobilized Enzymes Proteins 0.000 description 10
- 235000011187 glycerol Nutrition 0.000 description 8
- 238000000926 separation method Methods 0.000 description 8
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 239000012075 bio-oil Substances 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000000295 fuel oil Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000011973 solid acid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000005809 transesterification reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P30/00—Technologies relating to oil refining and petrochemical industry
- Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock
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Abstract
The invention discloses a method for preparing biodiesel through an immobilized lipase-porous membrane bioreactor. The hollow fiber porous membrane modules of immobilized lipase are assembled into an enzyme-membrane bioreactor, the admixture of grease and lower alcohol passes through the shell pass of the membrane modules, water passes through the tube pass of the membrane modules, after the grease and the lower alcohol are circularly reacted for a period of time under the catalytic action of the immobilized lipase, the grease and the lower alcohol can be transformed into the biodiesel. Because the method of the invention adopts the lipase-porous membrane as the medium between the grease phrase and the water phase, not only the on line byproduct glycerol separating can be performed, but also necessary water can be actively supplied to the immobilized lipase, therefore high enzyme activity and stability can be maintained, and biodiesel can be efficiently prepared, simultaneously solvent cleaning and batch charging are avoided, and the method has the advantages that the technology is simple, the continuous operation can be performed, the cost is lower, and the method meet the green chemical development trend.
Description
Technical field
The invention belongs to the membrane separation technique field.Be specifically related to a kind of polyalcohol stephanoporate membrane prepare method of bio-diesel oil of utilizing immobilized lipase.
Background technology
Biofuel is subjected to great attention both domestic and external as a kind of environment Friendly fuel oil.At present, the preparation method of bio-diesel oil mainly contains chemical synthesis, biological enzyme, supercutical fluid method and engineering microalgae method etc.Wherein immobilized enzyme method prepares that biofuel has efficient height, energy consumption low (reaction conditions gentleness), good, the pure consumption of selectivity is little, by product is few, environmental friendliness advantages such as (need not to carry out liquid waste disposal).But immobilized enzyme method exists by-product glycerin to make the stability decreases of enzyme and shortening in work-ing life, substrate low-carbon alcohol deprive the necessary water of enzyme and cause problems such as enzymic activity decline and even inactivation in the absorption of enzyme molecular surface again.
Adopt the immobilized enzyme polymer separation film to prepare biofuel and can make up the biocatalytic Activity of enzyme and the separation function of film, have the following advantages: 1. separatory membrane is the good carrier of enzyme immobilization, can effectively reduce product or substrate inhibition by original position separation or selective permeation; 2. in view of the porosity characteristic of separatory membrane, its mass transfer area is big, rate of mass transfer is fast; 3. can avoid emulsification and problems such as breakdown of emulsion, liquid flooding; 4. be easy to serialization, automatic control and integrated.People such as Guerreiro have reported the application of polymer separation film in the preparation biofuel, but employed polymeric film is mainly as solid acid catalyst, belong to chemical catalysis (Transesterification ofsoybean oil over sulfonic acid functionalised polymeric membranes, Catalysis Today, 2006,118:166-171).It is the method that two process couplings of propylene glycol are got up by membrane filtration technique with biodiesel manufacture and transformation of glycerol that CN1276962C discloses a kind of, but the main purpose of this invention is the coupling production propylene glycol.Up to now, there is not the polymer separation film of immobilized enzyme to be used for the report of biodiesel manufacture as yet, more do not have technology and the practical application that specifically to implement, be necessary that the novel immobilized lipase-porous membrane bio-reactor that development technology is simple and efficient is higher is used for biodiesel manufacture.
Summary of the invention
The purpose of this invention is to provide and a kind ofly prepare method of bio-diesel oil by the immobilized lipase-porous membrane bio-reactor, the tubular fibre porous-film membrane module of immobilized lipase is assembled into enzyme-film bioreactor, the mixture of grease and low-carbon alcohol is walked the shell side of membrane module, flow velocity is 1～20L/h, the while flow velocity is the tube side that the water of 1～10L/h is walked membrane module, promptly is converted into biofuel behind grease and the low-carbon alcohol circulating reaction 2～10h under catalytic action of immobilized lipase.With porous-film mutually and the medium between the water as grease, both can be for immobilized enzyme initiatively provided necessary water, again can the ON-LINE SEPARATION by-product glycerin, thus keep higher enzymic activity and stability.
This preparation method of bio-diesel oil comprises the steps:
(1) lipase is fixed in the tubular fibre porous film surface, the membrane module of preparation immobilized lipase is assembled into enzyme-film bioreactor then.
Described tubular fibre porous-film is polysulfones, polyacrylonitrile and polyvinylidene fluoride film etc., and molecular weight cut-off is 1～50kDa.
Described lipase can be the general lipase in this area, for example derives from the lipase of Candida antarctica, Rhizomucor miehei, Thermomyces lanuginosus, Burkholderia acepacia, Pesudomonas fluorescens, Aspergillus niger, Mucor miehei or Candida rugosa.
(2) mixture with 5L grease and low-carbon alcohol injects raw material storage tank 1, walks membrane module 3 shell sides through pump 2, and flow velocity is 1～20L/h.
Described grease is a bio-oil, comprises one or more the mixture in fish oil, lard, Viscotrol C, rapeseed oil, soybean oil, peanut oil, Semen Maydis oil, Oleum Gossypii semen, Rice pollard oil, algae grease, waste edible oil and the oil refining pin material;
Described low-carbon alcohol is methyl alcohol or ethanol or their mixture.
The volume ratio of described grease and low-carbon alcohol is 1: 1～1: 9.
(3) water is walked membrane module 3 tube sides by storage tank 6, and provides minor amount of water through hollow-fibre membrane for immobilized lipase, and the flow velocity of water is 1～10L/h, constantly circulation.
(4) under catalytic action of immobilized lipase, grease and low-carbon alcohol circulation 2～10h afterreaction generate biofuel, are collected in biofuel storage tank 5 through valve 4, obtain biofuel; By-product glycerin enters tube side by shell side, enters glycerine storage tank 9 through skimmer 8.
Effect of the present invention and benefit are with porous-film as the medium between grease phase and the water, both can the ON-LINE SEPARATION by-product glycerin, again can be for immobilized enzyme initiatively provide necessary water, thus keep higher enzymic activity and stability, can the efficient production biofuel.Simultaneously, avoided solvent cleaning and batch charging, but had the simple operate continuously of technology, cost is lower and meets advantage such as Green Chemistry developing direction.
Description of drawings
Fig. 1 prepares biofuel apparatus structure synoptic diagram for the present invention.
Embodiment
With reference to the embodiment of description of drawings technical solution of the present invention, wherein the mixture of grease in the raw material storage tank 1 and low-carbon alcohol is walked the shell side of immobilized enzyme membrane module 3 through pump 2, and the circulating reaction certain hour is after valve 4 enters biofuel storage tank 5; Water in the water storage tank 6 is walked the tube side of immobilized enzyme membrane module 3 through pump 7, and through skimmer 8 separatory, water cycle is used, and glycerine enters glycerine storage tank 9.Wherein shell side refers between the hollow-fibre membrane of immobilized enzyme membrane module 3 or the space between hollow-fibre membrane and the housing; Tube side refers to the hollow-fibre membrane internal space of immobilized enzyme membrane module 3.
Following examples are done more detailed description to the present invention, but described example is not construed as limiting the invention.
The polyacrylonitrile porous membrane (molecular weight cut-off 10kDa) of immobilized lipase (deriving from Candida antarctica) is made membrane module, link enzyme-film bioreactor.2.5L fish oil and 2.5L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 2L/h.Circulation 4h artifact diesel oil yield is about 91%.
The polysulfones porous-film (molecular weight cut-off 1kDa) of immobilized lipase (deriving from Rhizomucor miehei) is made membrane module, link enzyme-film bioreactor.2L lard and 3L alcoholic acid mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 10L/h.Circulation 4h artifact diesel oil yield is about 93%.
The polysulfones porous-film (molecular weight cut-off 10kDa) of immobilized lipase (deriving from Thermomyces lanuginosus) is made membrane module, link enzyme-film bioreactor.2L Viscotrol C and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 2L/h.Circulation 4h artifact diesel oil yield is about 92%.
Embodiment 4
The polysulfones porous-film (molecular weight cut-off 50kDa) of immobilized lipase (deriving from Burkholderia acepacia) is made membrane module, link enzyme-film bioreactor.2L soybean oil and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 20L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 10L/h.Circulation 2h artifact diesel oil yield is about 90%.
The polyvinylidene fluoride porous film (molecular weight cut-off 5kDa) of immobilized lipase (deriving from Pesudomonas fluorescens) is made membrane module, link enzyme-film bioreactor.0.5L peanut oil and 4.5L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 2L/h.Circulation 4h artifact diesel oil yield is about 92%.
The polysulfones porous-film (molecular weight cut-off 2kDa) of immobilized lipase (deriving from Aspergillus niger) is made membrane module, link enzyme-film bioreactor.2L Semen Maydis oil and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 4h artifact diesel oil yield is about 93%.
Embodiment 7
The inorganic ceramic film (molecular weight cut-off 2kDa) of immobilized lipase (deriving from Mucor miehei) is made membrane module, link enzyme-film bioreactor.2L Oleum Gossypii semen and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 4h artifact diesel oil yield is about 93%.
Embodiment 8
The inorganic ceramic film (molecular weight cut-off 1kDa) of immobilized lipase (deriving from Candida rugosa) is made membrane module, link enzyme-film bioreactor.2L Rice pollard oil and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 4h artifact diesel oil yield is about 91%.
Embodiment 9
The inorganic ceramic film (molecular weight cut-off 50kDa) of immobilized lipase (deriving from Candida antarctica) is made membrane module, link enzyme-film bioreactor.2L algae grease and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 4h artifact diesel oil yield is about 94%.
Embodiment 10
The polysulfones porous-film (molecular weight cut-off 2kDa) of immobilized lipase (deriving from Candida antarctica) is made membrane module, link enzyme-film bioreactor.2L waste edible oil and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 6h artifact diesel oil yield is about 95%.
Embodiment 11
The polysulfones porous-film (molecular weight cut-off 2kDa) of immobilized lipase (deriving from Candida antarctica) is made membrane module, link enzyme-film bioreactor.2L is refined oil pin material and 3L methanol mixture inject raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 6h artifact diesel oil yield is about 93%.
Embodiment 12
The inorganic ceramic film (molecular weight cut-off 5kDa) of immobilized lipase (deriving from Candida antarctica) is made membrane module, link enzyme-film bioreactor.2L rapeseed oil and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 4h artifact diesel oil yield is about 94%.
Embodiment 13
The polysulfones porous-film (molecular weight cut-off 2kDa) of immobilized lipase (deriving from Candida antarctica) is made membrane module, link enzyme-film bioreactor.1L fish oil, 1L Rice pollard oil and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 5L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 5L/h.Circulation 4h artifact diesel oil yield is about 91%.
Embodiment 14
The polysulfones porous-film (molecular weight cut-off 2kDa) of immobilized lipase (deriving from Candida antarctica) is made membrane module, link enzyme-film bioreactor.1L fish oil, 0.2L lard, 0.8L soybean oil and 3L methanol mixture are injected raw material storage tank 1, walk membrane module 3 shell sides through pump 2, flow velocity is 1L/h; Water is walked membrane module 3 tube sides by storage tank 6 through pump 7, and flow velocity is 1L/h.Circulation 10h artifact diesel oil yield is about 94%.
Claims (6)
1. one kind prepares method of bio-diesel oil by the immobilized lipase-porous membrane bio-reactor, it is characterized in that: lipase is fixed in the tubular fibre porous film surface, the membrane module of preparation immobilized lipase, be assembled into enzyme-film bioreactor then, the mixture of grease and low-carbon alcohol is walked the shell side of membrane module, flow velocity is 1～20L/h, the while flow velocity is the tube side that the water of 1～10L/h is walked membrane module, promptly is converted into biofuel behind grease and the low-carbon alcohol circulating reaction 2～10h under catalytic action of immobilized lipase.
2. prepare method of bio-diesel oil by claim 1 is described by the immobilized lipase-porous membrane bio-reactor, it is characterized in that described lipase is the lipase that derives from Candida antarctica, Rhizomucor miehei, Thermomyces lanuginosus, Burkholderia acepacia, Pesudomonas fluorescens, Aspergillus niger, Mucor miehei or Candida rugosa.
3. prepare method of bio-diesel oil by claim 1 is described by the immobilized lipase-porous membrane bio-reactor, it is characterized in that described tubular fibre porous-film is polysulfones, polyacrylonitrile and polyvinylidene fluoride film or inorganic ceramic film, the molecular weight cut-off of film is 1～50kDa.
4. prepare method of bio-diesel oil by claim 1 is described by the immobilized lipase-porous membrane bio-reactor, it is characterized in that described grease is a kind of or its mixture in fish oil, lard, Viscotrol C, rapeseed oil, soybean oil, peanut oil, Semen Maydis oil, Oleum Gossypii semen, Rice pollard oil, algae grease, waste edible oil and the oil refining pin material.
5. prepare method of bio-diesel oil by claim 1 is described by the immobilized lipase-porous membrane bio-reactor, it is characterized in that described low-carbon alcohol is methyl alcohol or ethanol or their mixture.
6. prepare method of bio-diesel oil by claim 1 is described by the immobilized lipase-porous membrane bio-reactor, the volume ratio that it is characterized in that described grease and low-carbon alcohol is 1: 1～1: 9.
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| CN2008100605494A CN101265413B (en) | 2008-03-27 | 2008-03-27 | Method for preparing biological diesel oil from immobilized lipase-porous membrane biological reactor |
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| CN101265413B true CN101265413B (en) | 2011-11-02 |
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| CN101514293B (en) * | 2009-03-31 | 2012-05-30 | 南京工业大学 | Method for preparing biodiesel by membrane integrated reactor |
| CN103602656A (en) * | 2013-11-12 | 2014-02-26 | 柳州市净元生物科技有限公司 | Method for preparing immobilized enzymes and immobilized strains |
| RU2665041C2 (en) * | 2016-12-30 | 2018-08-27 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский государственный университет нефти и газа (национальный исследовательский университет) имени И.М. Губкина" | Method for obtaining biodiesel fuel |
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|---|---|---|---|---|
| CN1648207A (en) * | 2004-12-23 | 2005-08-03 | 大连理工大学 | Process for coupling producing bioloigical diesel oil and 1,3-propylene glycol |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1648207A (en) * | 2004-12-23 | 2005-08-03 | 大连理工大学 | Process for coupling producing bioloigical diesel oil and 1,3-propylene glycol |
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