CN101264336A

CN101264336A - Collagen base composite sponge and preparation thereof

Info

Publication number: CN101264336A
Application number: CNA2007100892418A
Authority: CN
Inventors: 原彬
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-03-16
Filing date: 2007-03-16
Publication date: 2008-09-17

Abstract

The invention discloses a collagen-based compound sponge and the preparation method, belonging to the technical field of medical materials and the preparation method, which aims to provide low priced collagen-based compound sponges with perfect wound surface hemostasis and repairing effect, and the preparation method. The collagen-based compound sponge comprises the following materials according to the proportion of weight fractions: collagen 50 to 90; chitosan 8 to 48; and glycerol 0.5 to 5. The preparation method of the collagen-based compound sponge comprises the following steps: 1) making the collagen be dissolved in acetic acid solution with concentration of 0.01 to 0.1 percent to obtain collagen solution with concentration of 1 to 3 percent; 2) making the chitosan be dissolved in acetic acid solution with concentration of 0.01 to 0.1 percent to obtain chitosan solution with concentration of 1 to 3 percent; 3) making the collagen solution, the chitosan solution and glycerol be uniform mixed according to the proportion of weight account: 50 to 90 : 8 to 48 : 0.5 to 5, and then be freezed for 1 to 5 days under the temperature of -10 to -80 DEG C, to obtain spongy materials after freeze-drying; 4) making the spongy material in the gas generator, and be fixed for 20 to 100 minutes through cross-linking agent of aldehydes materials under the temperature of 40 to 50 DEG; 5) vacuum drying to obtain collagen based sponge after washing through the glycerol solution.

Description

Collagen base composite sponge and preparation method thereof

Technical field:

The present invention relates to material of a kind of medical grade and preparation method thereof, particularly relate to a kind of collagen base composite sponge and preparation method thereof.

Background technology:

Collagen protein is the rich in protein of animal body intensive amount, is the main component of extracellular matrix (ExtracellularMatrix).Collagen protein have antigenicity low, big with the cell affinity, produce characteristics such as hemagglutinative function with platelet, thereby be widely used at field of medicaments.Have been found that 20 kinds of collagen protein types that gene are different at present, its maximum feature is the structure of three collagen protein spirals.With the maximum type i collagen albumen of amount is example, and about 95% of collagen molecules is three coiled strand fields, and remaining about 5% is the terminal peptide chain structure field of noncollagen protein.The antigenicity of collagen protein is low, mainly is because three coiled strand fields of collagen protein lack tyrosine residue, the outer main peptide chain from collagen protein N and C terminal of the antigenicity removal of impurity of collagen protein product in the market.Research and development based on the curable product of collagen protein, having obtained a lot of achievements, mainly is that collagen protein is made different dosage form, as solution, gel, powder, sponge, fiber, thin film etc., or carry out various modifications, make it have different medical or medical functions.But existing collagen protein sponge suppleness is relatively poor on the market, and it is very limited in the application of soft tissue wound surface (reparation is filled in the hemostasis as the department of cerebral surgery wound surface).In addition, price factor also is one of condition of coming into the market of restriction collagen protein series products scale.

Summary of the invention:

It is good with repairing effect to the purpose of this invention is to provide a kind of wound surface hemostasis, the collagen base composite sponge that price is lower.

For achieving the above object, the present invention by the following technical solutions.

A kind of collagen base composite sponge, it is made up of the material of following ratio of weight and number: the described collagen protein of collagen protein 50-90 chitosan 8-48 glycerol 0.5-5 can be commercially available collagen protein primary product (mainly being type i collagen albumen), also can be to adopt following method preparation: animal tissue's machinery that 1) will be rich in collagen protein be removed impurity such as epidermal area, muscle and fat, cleans up after the pulverizing.2) dilute alkaline soln and the defat of alcohols material solution washing of usefulness pH8.5-10.5, the excellent sodium oxide of dilute alkaline soln wherein: sodium bicarbonate: water: 0.1-1: 5-10: 89-94.9, alcohols material aqueous solution preferred alcohol: isopropyl alcohol: water two 5-10: 2-5: 85-93:3) be dissolved in the dilute acid soln of pH5.5-6.5, wherein preferable formic acid, acetic acid, propanoic acid or hydrochloric acid; 4) in above-mentioned solution, add compound protease, the ratio of weight and number of collagen protein and compound protease is 100-1000: 1, under the 30-35 ' C stir culture 10-15 hour: 5) in the supernatant of centrifugal collection, add NaCl, make concentration reach 2mol/L, 0-10 ℃ was stirred 10-20 hour down, collects the precipitate that obtains; 6) precipitate is dissolved in 0.1-0.5% acetic acid solution cattle, centrifugal collection supernatant; 7) add NaCl in the supernatant again, make concentration reach 1mol/L, stir and obtain precipitate; Repetitive operation 3-4 time obtains the purpose product.

More excellent for the quality that makes collagen base composite sponge, described chitosan is selected for use and is taken off that the acyl degree is 70-90%, and viscosity-average molecular weight is the product of (1.0-4.0) * 10.

Claims

Collagen base composite sponge and preparation method thereof it form by the material of following ratio of weight and number: the described collagen protein of collagen protein 50-90 chitosan 8-48 glycerol 0.5-5 can be commercially available collagen protein primary product (mainly being type i collagen albumen), also can be to adopt following method preparation: animal tissue's machinery that 1) will be rich in collagen protein be removed impurity such as epidermal area, muscle and fat, cleans up after the pulverizing.2) dilute alkaline soln and the defat of alcohols material solution washing of usefulness pH8.5-10.5, the excellent sodium oxide of dilute alkaline soln wherein: sodium bicarbonate: water: 0.1-1: 5-10: 89-94.9, alcohols material aqueous solution preferred alcohol: isopropyl alcohol: water two 5-10: 2-5: 85-93:3) be dissolved in the dilute acid soln of pH5.5-6.5, wherein preferable formic acid, acetic acid, propanoic acid or hydrochloric acid; 4) in above-mentioned solution, add compound protease, the ratio of weight and number of collagen protein and compound protease is 100-1000: 1, under the 30-35 ' C stir culture 10-15 hour: 5) in the supernatant of centrifugal collection, add NaCl, make concentration reach 2mol/L, 0-10 ℃ was stirred 10-20 hour down, collects the precipitate that obtains; 6) precipitate is dissolved in 0.1-0.5% acetic acid solution cattle, centrifugal collection supernatant; 7) add NaCl in the supernatant again, make concentration reach 1mol/L, stir and obtain precipitate; Repetitive operation 3-4 time obtains the purpose product.