CN101259043A - Embryo transplantation acceptor cattle estrus synchronization method - Google Patents
Embryo transplantation acceptor cattle estrus synchronization method Download PDFInfo
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- CN101259043A CN101259043A CNA2008101042839A CN200810104283A CN101259043A CN 101259043 A CN101259043 A CN 101259043A CN A2008101042839 A CNA2008101042839 A CN A2008101042839A CN 200810104283 A CN200810104283 A CN 200810104283A CN 101259043 A CN101259043 A CN 101259043A
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Abstract
The invention relates to a method of estrus synchronization of embryo transplantation receptor cow, which belongs to the field of embryo engineering. The method of estrus synchronization of embryo transplantation receptor cow is characterized in that: Cue-Mate (vaginal suppository) is put into the body of the receptor cow on any day of estrus circle of the cow, then the cow is injected with 100microgram of GnRH and the day is set as a first day; on a fifth day to a seventh day, the vaginal suppository is taken out and the cow is injected with 500microgram of PG; two days later, the cow ovulates; and the transplantation is carried out 7 to 7.5 days after the ovulation. Using the method, time for treating the cow is shortened; application efficiency of the cow is enhanced and breeding cost is saved; the ovulation rate of the cow after the treating is enhanced to 85 to 90 percent from the original 75 to 80 percent.
Description
Technical field
The present invention relates to the embryo engineering field, particularly relate to and utilize estrus synchronization to carry out embryo transfer technology.
Background technology
Embryo transfer is that the body early embryo after the fine breed cow breeding is taken out, and is transplanted in the identical milch cow body of other end physiological status of the same race, makes it to continue the bud into technology of body one by one.Because cattle economic worth height, belong to monotocous animal, farrowing quantity is few, and the breeding cycle is long, carries out with embryo transfer technology that purebred expansion group breeds and the cattle improved effect is good, and speed is fast.Use the technology that some hormone or medicine and livestock management measure Artificial Control jenny colony oestrus and ovulate at one time, be called estrus synchronization (estrus synchronization).The ultimate principle of estrus synchronization is by regulating the estrus cycle, the ovulation generation at one time of oestrusing of control animal population.Make the method that prolongs or shorten luteal phase, can control the generation of follicle or the formation of corpus luteum, all can make animal reach estrus synchronization and arrange and ovulate.Estrus synchronization hormone commonly used is GnRH, progestogen, PG etc.GnRH can estrus induction on jenny, ovulation, improves the breeding conception rate, also can be used for treating diseases such as the static and follicular cyst of cattle ovary; The major function of progestogen is to promote embryo nidation and keep gestation by stimulating the endometrium glandular secretion and suppressing the uterus muscle contraction; PG all has dissolution to the corpus luteum on the cattle ovary.Embryo transfer technology is in conjunction with the superovulation technology, and is significant for the breeding potential that excavates outstanding female animal, and expanding propagation high yield cow (cow) increases high yield cow quantity fast.At present the method for embryo transfer mainly is that cattle was put into Cue-Mate (vaginal suppository) in any one day in the estrus cycle, and the recession bolts were also injected PG in 9～11 days, oestruses after removing bolt 2d, transplants behind the 7d that oestruses.Only handling early stage to transplant approximately to the end from cattle like this needs 18～20 days, and the processing time is longer, has caused the recipient cattle access times to reduce, and has increased feeding cost.
Summary of the invention
At above-mentioned shortcoming, the present invention improves the embryo transplantation acceptor cattle estrus synchronization method, has shortened a cattle processing time, has improved cattle application efficiency only, has saved feeding cost.
The method of embryo transplantation acceptor cattle estrus synchronization, it is characterized in that: recipient cattle in the estrus cycle cattle put into Cue-Mate (vaginal suppository) in any one day in the estrus cycle, and inject 100 μ g GnRH, be made as the 1st day, removed bolt at the 5th～7 day and inject 500 μ gPG, cattle only oestruses after 2 days; Transplanted in 7-7.5 days after oestrusing.
Described to remove the bolt time be the 5th day, and transplant time is for oestrusing back the 7th day.
This method was put into Cue-Mate in any one day in the estrus cycle, put into Cue-Mate in the described method and inject GnRH simultaneously, promoted the growth of follicle on the cattle ovary; Removed bolt at 5-7 days, shift to an earlier date 4～5 days than conventional method; Remove bolt in the described method and inject PG simultaneously, promote cow follicle maturity and ovulation, recipient cattle is oestrused after 2 days, and transplanted in 7-7.5 days the back of oestrusing.
After this method processing, shortened the cattle processing time only, improved cattle application efficiency only, saved feeding cost, handle the back cattle rate of only oestrusing and also bring up to 85%～90% by original 75%～80%.
The specific embodiment
The present invention is described in further detail below by embodiment.
Embodiment 1
1 reagent
(1.1PG Cloprostenol)
1.2GnRH gonadotropin releasing hormone
1.3Cue-Mate vaginal suppository
1.4 lidocaine hydrochloride
2 equipment
2.1 France's card Su Yizhi rifle is supporting
2.2 it is supporting that rifle is planted in the Japanese fuji translation
2.3 Shearing shears (18cm)
2.4 tubule is cut
2.5 staight scissors (18cm)
3 embryo transplantation acceptor cattles are prepared
3.1 reproductive physiology health, non-reproductive system disease, free from infection.
3.2 the estrus cycle is normal, the ovulation of normally oestrusing forms corpus luteum.
3.3 do not have the difficult labour history, do not have the stream history.Do not have and join infertile record for a long time.
3.4 more than build was medium, body weight reached 350kg, the growth of livestock is better-than-average.Good bloom.
3.5 it is normal directly to examine the reproductive system structure, function is normal, and the flexible nothing in uterus is fast firmly.The ovary quality is flexible, normal development follicle or periodicity corpus luteum is arranged, volume 1 * 1 * 1～4 * 4 * 4cm.
3.6 the mucus that the no dirt of outward appearance vaginal orifice cleaning is discharged or discharged is limpid transparent, free from extraneous odour.
4 processing of oestrusing
3.1 processing method: the cattle of selecting to be fit to do recipient cattle was only put into Cue-Mate (vaginal suppository) in any one day cattle in the estrus cycle, and injects 100 μ g GnRH, was made as 1d.5d removes bolt and injects 500 μ gPG, and cattle only oestruses after 2 days.The back 7d that oestruses transplants.
3.2 observe the standard of oestrusing
3.2.1 2d begins to observe and oestruses behind injection PG.
The Niu Yinmen redness 3.2.2 oestrus flows limpid hyalomucoid, accepts other cattle mountings and plants oneself.
4 transplant the selection with the embryo
4.1 embryo quality grade classification
4.1.1A the top-quality embryo of level.The embryonic cell symmetry, outward appearance is spherical in shape, and the spilting of an egg is even, good colour, the fetal development stage is consistent age with embryo.Blastomere more than 85% is complete.
4.1.2B the level quality is the embryo preferably.An amount of irregular cell is arranged in the zona pellucida, and the complete even good colour of most of blastomeres has at least 50% blastomere complete.
4.1.3C the ropy embryo of level.A large amount of irregular cells have only a few cell complete, and at least 25% blastomere is complete.
4.1.4D level is dead or that degenerate and unfertilized embryo, can not grow.
4.2 quality is selected:
4.2.1 generally select A level embryo to be used for freezing or bright embryonic implantation.
4.2.2B the level embryo generally is directly used in bright embryonic implantation.
4.2.3C the embryo of level and D level then is not suitable for transplanting and freezing.
5 embryo transfers
5.1 transplant time: with the same day of oestrusing be 0d, transplant in the 7th～7.5d
5.2 prepare before transplanting
5.2.1 determine transplant recipient: the examination per rectum beefsteak ovum situation of oestrusing, corpus luteum grows whether meet the transplanting standard, determines transplantation site, determines the fetal development stage.
5.2.1.1 transplant recipient corpus luteum choice criteria
5.2.1.1.1 recipient cattle is oestrused can normally ovulation in 12～36h.
5.2.1.1.2 ovulation back the 6th～7d checks corpus luteum.
5.2.1.1.3 the outstanding ovary surface configuration of corpus luteum be regular mushroom-shaped, the volcano shape of the mouth as one speaks, tapered or column type, volume 0.8 * 0.8 * 0.8～3 * 3 * 3cm then can be elected to be receptor.
5.2.1.1.4 corpus luteum protuberate part is less, but the part that is embedded in ovary inside surpass the ovary volume 1/3rd also be chosen as transplant recipient.
5.2.2 prepare in receptor Baoding.
5.2.3 recipient cattle anesthesia
5.2.3.1 position: recommend the epidural of tail gap.
5.2.3.2 dosage: lidocaine hydrochloride 40mg/2ml * 2.
Take off iodine 5.2.3.3 recommend the cotton sterilization of tail gap location cropping-→ 75% alcohol swab defat-→ 5% iodine tincture-→ 75% alcohol swab.
Recommend the epidural of tail gap location 5.2.3.4 anesthetic syringe vertically thrusts, slowly push.
5.2.3.5 it is reactionless for reaching anaesthetic effect to touch the cattle perineal position.
Prepare with the embryo 5.2.4 transplant
5.2.4.1 water filling in the frozen embryo transfer, heat-preserving container, water temperature is controlled at 35 ℃.
5.2.4.2 from liquid nitrogen container, take out the embryo who to transplant, air bath 10s, water-bath 10-15s..
5.2.4.3 with outer water stain the drying of embryo's tubule, write down the full detail on the tubule with absorbent paper.
5.2.4.4 cut at distance tubule sealing end 5～6cm with tubule and to cut off, embryo's tubule is inserted transplant in the rifle, put holster, fastening prepares to transplant.
5.2.4.5 bright embryonic implantation.The embryo through evaluation grading determine available after, clean tubulature then, i.e. transplantation earlier.
Annotate: the method difference of embryo cryopreservation, the method for thawing are also different.Different freezing procedures generally all has supporting thaw routine.
Above embryo's preparation method only is used for reference.
5.3 graft procedure
5.3.1 hands go into rectum remove stool-→ clear water cleans outer dirt-→ 1 ‰ potassium permanganate solution of vaginal orifice or 1 ‰ bromo geramine solution disinfections are handled-→ sterilization absorbent paper dries.
5.3.2 the assistant breaks Niu Yinmen into two with one's hands, the patient will transplant rifle and insert vaginal orifice, send into obliquely upward earlier vagina first three/one, lift the flat through cervical orifice in vagina bottom of sending into then.
5.3.3 patient's internal rectum hands lifts the cervix uteri outer end, another hand-held rifle of transplanting.Both hands cooperations will be transplanted rifle and be imported cervix uteri, through transplanting side cornua uteri first three/place.
5.3.4 evenly the embryo is pushed the uterus at last, the light and slow transplanting rifle of withdrawing from.Graft procedure is finished.
5.4 points for attention:
5.4.1 will determine to oestrus accurately when recipient cattle is checked.
5.4.2 selecting corpus luteum will press the corpus luteum choice criteria selects to judge.
5.4.3 Baoding is certain.
5.4.4 anesthesia accurate and effective.
5.4.5 exopathogenic factor portion cleans up, sterilization is careful, thoroughly.
5.4.6 light and slow when transplanting the rifle importing, prevent to damage cervix uteri and endometrium.
5.4.7 to keep at the uniform velocity light and slow when pushing the embryo, can not be too fast too quickly.
5.4.8 it is light and slow that the transplanting rifle will remain a constant speed when withdrawing from.
5.4.9 will make cattle in the migration process keeps quite.Transplanting action wants efficiently.Minimizing stress.
6 pregnancy checks
6.1 the pregnancy check time: transplant latter two month and carry out.
6.2 pregnancy check method: rectum touches to be checked.
Calculate expected date of confinement 6.3 determine the gestation back
6.3.1 subtracting for three gained dates, the moon umber on transplanting date is expected date of confinement.
6.3.2 transplant recipient is oestrused the date, month subtracts three, and the date adds six, and the gained date is expected date of confinement.
The contrast of embodiment 2 the inventive method and conventional method, conventional method is just being put into Cue-Mate (vaginal suppository), the time do not inject GnRH, removing the bolt time is 9-11 days, all the other operations are all identical.
Pregnant situation behind the effect of estrus synchronization and the embryo transfer
| Handle | The cattle number same period (head) | The rate of oestrusing % | Pregnancy rate % |
| The inventive method | 68 | 88.2％ a (60/68) | 46.7％ a (28/60) |
| Conventional method (matched group) | 50 | 76％ a (38/50) | 48.7％ a (19/39) |
After this method processing, shortened the cattle processing time only, improved cattle application efficiency only, saved feeding cost, handle the back cattle rate of only oestrusing and also bring up to 85%～90% by original 75%～80%.
Claims (2)
1, the method for embryo transplantation acceptor cattle estrus synchronization, it is characterized in that: recipient cattle in the estrus cycle cattle put into vaginal suppository in any one day in the estrus cycle, and inject 100 μ gGnRH, be made as the 1st day, removed bolt at the 5th～7 day and inject 500 μ gPG, cattle only oestruses after 2 days; Transplanted in 7-7.5 days after oestrusing.
2, method according to claim 1, it is described that to remove the bolt time be the 5th day, transplant time is for oestrusing back the 7th day.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008101042839A CN101259043A (en) | 2008-04-17 | 2008-04-17 | Embryo transplantation acceptor cattle estrus synchronization method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008101042839A CN101259043A (en) | 2008-04-17 | 2008-04-17 | Embryo transplantation acceptor cattle estrus synchronization method |
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| CN101259043A true CN101259043A (en) | 2008-09-10 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613423A (en) * | 2016-03-08 | 2016-06-01 | 中国农业科学院北京畜牧兽医研究所 | Livestock synchronous ovulation and fixed-time artificial insemination method |
| TWI602550B (en) * | 2016-07-22 | 2017-10-21 | 國立屏東科技大學 | Smart cells syringe of cattle ovary |
| CN114948325A (en) * | 2021-12-31 | 2022-08-30 | 江苏省农业科学院 | Follicle development treatment method for improving cow mating conception rate |
-
2008
- 2008-04-17 CN CNA2008101042839A patent/CN101259043A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105613423A (en) * | 2016-03-08 | 2016-06-01 | 中国农业科学院北京畜牧兽医研究所 | Livestock synchronous ovulation and fixed-time artificial insemination method |
| TWI602550B (en) * | 2016-07-22 | 2017-10-21 | 國立屏東科技大學 | Smart cells syringe of cattle ovary |
| CN114948325A (en) * | 2021-12-31 | 2022-08-30 | 江苏省农业科学院 | Follicle development treatment method for improving cow mating conception rate |
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