CN101254017A - Fresh-keeping article containing nano silver, method of preparing the same and use - Google Patents
Fresh-keeping article containing nano silver, method of preparing the same and use Download PDFInfo
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- CN101254017A CN101254017A CNA2007100796514A CN200710079651A CN101254017A CN 101254017 A CN101254017 A CN 101254017A CN A2007100796514 A CNA2007100796514 A CN A2007100796514A CN 200710079651 A CN200710079651 A CN 200710079651A CN 101254017 A CN101254017 A CN 101254017A
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Abstract
The invention relates to a fresh-keeping article containing nanometer silver and a preparation method of the fresh-keeping article. In addition, the fresh-keeping article containing the nanometer silver in the invention has strong and long-acting sterilizing and deodorizing properties, so that the invention also relates to the application of the fresh-keeping article containing the nanometer silver. The application comprises the fresh-keeping and preservation of various foods and agricultural products, and the increase of the survival rate of the aquaculture animals.
Description
Technical field
The invention relates to a kind of fresh-keeping article that comprises Nano Silver, with and preparation method thereof and be used for the purposes of fresh-keeping foodstuff and agricultural products.The fresh-keeping article that comprises the Nano Silver particulate among the present invention has powerful and long-acting sterilization and deodorizing character, can be used for preserving normal food for example vegetables, fruit, seafood, meat etc. and agricultural products such as fresh flower and plant, and can increase the survival rate of aquiculture animal.
Background technology
Because metal has redox ability, therefore many metals comprise the known killing bacteria that can be used for of silver, copper, mercury and zinc, but some metal is excessive because of activity, but to mankind's toxigenicity, for example mercury may be fatal to human body, therefore must carefully be elected to be the natural antibacterial material that uses into the mankind.Moreover, use antibiotic to produce many problems or had some shortcomings for a long time as bactericide, except easy generation resistance, antibiotic is because its different antimicrobial spectrum can only have antibacterial action to a few bacterium usually, and antibiotic sterilization process and internal metabolism process can produce damage for internal organs in the organism.Because the processing of metal can not cause resistance to bacterium, therefore compared to traditional antibiotic, metal has its superior characteristic on bacteria growing inhibiting and breeding or killing bacteria.
The mechanism of action of general anti-biotic material mainly is to be following aspect: (1) interference cell wall synthetic, and by suppressing polysaccharide chain and the crosslinked binding of tetrapeptide in the bacteria cell wall, so that cell membrane loses integrality and loses protective effect to osmotic pressure.(2) damaging cells film makes bacterium death by destroying cell membrane.(3) CKIs matter is synthetic, makes bacterium death by change or the building-up process that stops protein.(4) RNA is synthetic, comprises synthesizing of DNA and RNA etc. by the hereditary information that hinders bacterium.
In the antibacterial metal material, silver is considered to safety, wide spectrum and effective antiseptic.Also pointed out that Nano Silver has natural sterilizing ability (Journal of Biomedical Materials Research52 (4): 662-8,2000) on the document, can kill nearly microorganism such as bacterium more than 650 kinds and virus.Because increase rapidly through the silver surface after the nanometer processing, surface texture changes, sterilizing ability can thereby improve.The sterilization principle of Nano Silver is, because nano silver particles and cells of microorganisms wall/film have great binding ability, after positively charged nano-Ag particles touches electronegative microbial cell, absorption each other, nano-silver ionic can directly enter thalline, with mercapto in the epicyte protein enzyme (SH) combine, the blocking-up metabolism also loses activity it, but therefore bacteria growing inhibiting to reach sterilization effects and can not work the mischief to human body.Moreover most medicines can run off rapidly with after bacterium combines, but nano silver particles can not run off behind the degerm and still exist behind degerm, thereby sustainable and other bacterial action, therefore reach potent and long-acting sterilization purpose, the also superinfection of microorganism such as bacteriological protection in advance.
Nano Silver can improve tradition and use antibiotic shortcoming, remove not can diseaseful drug resistance, owing to the has a broad antifungal spectrum of Nano Silver, can bactericidal action be arranged to many different types of bacteriums, in addition, the using dosage of Nano Silver extremely low (in microgram) can be not toxic to animal or human's body.Therefore, Nano Silver has been used on many daily living articles, comprises Textile Fibre Product (for example mouth mask, socks, wound apply material etc.), household wares (for example air-conditioning, washing machine and refrigerator etc.), cosmetic or cleaning supplies (for example antibiotic with emulsion, toothpaste etc.), construction material (for example antibiotic furniture etc.) or plastic articles (for example PP, PE, PET, ABS etc.) etc.
Various forms of silver are widely studied for the different application field as liquid, powder and crosslinking mixture etc. and use.In liquid state, be difficult for handling and the limited problem of purposes for solving silver ion, also develop the many crosslinking agents and the solid support thing that silver ion, for example, United States Patent (USP) 5824267 is to disclose a kind of plastic substance with sterilization surface, some of them sterilization particle is to be exposed under this surperficial situation by embedding at each sterilization particulate fraction, and this sterilization particle mainly is made up of the silver metal particles of 0.0001 to 0.1 micron of the pottery of 0.01 to 0.5 micron of average diameter or alkali metal particles and the average diameter that is fixed thereon.Yet, use this class solid support thing of synthetic polymer material to need silver or silver ion and polymer bond or crosslinked usually, it may cause allergic reaction in the patient, so this class material can not be widely used in medical science and the health care.In addition, owing to lack and enough the contacting of bacterium, this class material also can't provide high bactericidal activity, and again because of silver ion can separate on the solid support thing, the bactericidal activity of this class material can reduce fast, therefore also can't manifest its sterilization effects for a long time.
At present, the plastic articles that generally contains Nano Silver is to make the Nano Silver in the solution make the plastic master batch that contains Nano Silver in general plastic pellet surface formation Nano Silver film by spraying or impregnation method, but the contained Nano Silver density of made plastic master batch is not high and can't uniformly dispersing, its sterilization effects thereby be affected.
In addition, on fresh food such as vegetable and fruit fresh-keeping, because this based food is very easily rotten after gathering, so it is fresh-keeping and be not easy.For prolonging storage period, various countries have developed multiple preservation technique, and for example, microwave is fresh-keeping, pressurization is fresh-keeping, microorganism is fresh-keeping, pressure-reducing fresh-keeping, electronic technology is fresh-keeping or use the edible fresh-ness preserving agent for vegetable and hydrocarbon mixture fresh-keeping etc.In addition, the preservative film or the bag that are used for fresh-keeping vegetables and fruits based food have been researched and developed, comprise the fresh-keeping plastic packaging film of a kind of hygroscopicity, it has than the translucent nylon membrane of highly permeable by two forms, and the high granulated sugar syrup of natural paste and osmotic pressure is housed between the film, can slowly absorb the moisture that oozes out from the vegetables and fruits surface, to reach preservation; And ceramic freshness protection package, its inboard in bag is coated with last layer ceramic masses as thin as a wafer, the far infrared that discharges via pottery can with contained humidity generation covibration in the vegetables and fruits, thereby promote the fresh-keeping of vegetables and fruits.Yet fresh-keeping for this based food used the fresh-keeping article of the Nano Silver that contains specially treated on the market and not.
In addition, because general commercially available fresh-keeping article still can't provide food and agricultural products longer-term effectively to preserve and fresh-keeping effect, also can't increase the survival rate of aquiculture animal.Therefore, for having the demand that good sterilization and deodorant activities and environmentally safe fresh-keeping article still have height.
Summary of the invention
The object of the present invention is to provide a kind of fresh-keeping article that comprises Nano Silver, with and be used for the purposes of fresh-keeping foodstuff and agricultural products.
In one aspect of the invention, the invention relates to the fresh-keeping article that comprises the Nano Silver particulate, wherein the Nano Silver particulate is present in the plastics of fusion even with it with 0.1 to 0.8% amount, and it can be different size and shape according to the purpose that will reach.In the fresh-keeping article of the present invention contained Nano Silver particulate do not have migration (migration) but phenomenon and have sterilization and the effect of deodorizing, therefore can provide food and agricultural products such as vegetables and fruit are potent and long fresh-keeping and deodorizing effect.
Contained Nano Silver is to exist with fine-grained form in the fresh-keeping article of the present invention, this contains antibiotic particulate (the nanosilver-containing antibacterial and antifungal granules of Nano Silver, NAG) in, contained nano-Ag particles firmly and fifty-fifty is attached on the stem marrow (stalkmarrow) of rush (Juncus effuses L.), the diameter of nano-Ag particles is about 1 to 100 nanometer, and indivedual nano-Ag particles has the argent core that oxidized silver surrounds.
The antibiotic particulate (NAG) that contains Nano Silver among the present invention is by the method manufacturing that comprises the following steps: (1) is cut into several piece with the stem marrow of rush; (2) the stem marrow with stripping and slicing is soaked in the solution that contains nano-Ag particles, so that nano-Ag particles is attached on the stem marrow of stripping and slicing; (3) after adhering to, according to circumstances with hot water and cold water washing stem marrow; (4) will adhere to the stem marrow drying of Nano Silver; And the stem marrow that (5) will contain nano-Ag particles grinds to form appropriate size to make NAG.
In a preferred embodiment, the antibiotic particulate (NAG) that contains Nano Silver of the present invention is by the method preparation that comprises the following steps: (1) is cut into several piece (about 0.5 to 2 centimetre of length) with the stem marrow of rush; (2) solution of preparation nano-Ag particles; (3) the stem marrow of stripping and slicing is soaked and thoroughly be mixed in the solution that contains nano-Ag particles, so that nano-Ag particles is attached on the stem marrow of stripping and slicing; (4) the stem marrow of washing stripping and slicing (preferably first with hot water, then with cold water washing); (5) with the stem marrow drying of stripping and slicing; And (6) grind to form the stripping and slicing stem marrow of drying the size of required NAG.Preferred NAG has can be by the size of No. 200 sieve apertures.In addition, for removing unwanted water-soluble substances, the stem marrow of stripping and slicing can be boiled, follow the stripping and slicing stem marrow that heat drying boils, the stem marrow with stripping and slicing is soaked in the solution that contains Nano Silver then.Preferably, soak the stripping and slicing stem marrow of Nano Silver with heat treatment, up to stripping and slicing stem marrow be brown till, then again with hot water and cold water washing stripping and slicing stem marrow.
In the antibiotic particulate (NAG) that contains Nano Silver of the present invention, contained nano-Ag particles be via silver nitrate is dissolved in ammoniacal liquor and add reducing agent in regular turn and oxidant in solution and make.Depend on the circumstances, can add NaOH in solution adjusting pH value, and can add ethanol solubility with enhancement solution in solution.Preferred reducing agent is glucose or ascorbic acid, and preferred oxidant is a hydrogen peroxide.
In a preferred embodiment, Nano silver solution is by the method preparation that comprises the following steps: (1) is with silver nitrate (AgNO
3) crystallization is dissolved in the ammonia spirit; (2) add as the glucose of reducing agent or ascorbic acid to this solution; And (3) add oxidant (being preferably hydrogen peroxide) to this solution.Depend on the circumstances, can add NaOH and ethanol solubility in solution respectively to adjust the pH value and to promote solution.
The antibiotic particulate (NAG) that contains Nano Silver of the present invention is to get by nano-Ag particles being attached to plant rush fritter.Adhering to of nano-Ag particles can confirm that it shows that nano-Ag particles is about 25 nanometers and is evenly distributed on the NAG at the diameter on the NAG via the flying-spot microscope that uses AMRAY1910FE and TN-8502.The content of nano-Ag particles on NAG is by the argentometry analysis, and every gram NAG contains 20 to 100 milligrams the silver of having an appointment.
In another aspect of this invention, the present invention also contains the method for the fresh-keeping article of Nano Silver about preparation, the antibiotic particulate that wherein contains Nano Silver is evenly to distribute with the plastics fusion on it, make the various fresh-keeping articles that contain Nano Silver through the conventional molded method again, for example preservative film, freshness protection package and crisper etc.In the inventive method the plastics that use including, but not limited to ABS, PE, PP, PS, PET, PC, DPP, CPP etc.In a preferred embodiment, the fresh-keeping article that comprises Nano Silver that method of the present invention is prepared is preservative film/bag, it is that antibiotic particulate (NAG) by will containing Nano Silver is with the plastics fusion and then process and make, wherein use 0.1 to 0.8 weight % to contain the antibiotic particulate and the even fusion of plastics of Nano Silver, weight with plastics is benchmark,, Nano Silver is able to reach the effect of bactericidal nano-silver so that can evenly distributing via contact on plastics, and then the gained admixture made film via the technology of generally extruding or blow out moulding, and according to required specification and folding and/or be cut into bag.
In another preferred embodiment of the inventive method, the amount that is used for adding with the plastics fusion the antibiotic particulate that contains Nano Silver is 0.4 weight %, and about 0.02 millimeter to 0.15 millimeter of the thickness of prepared preservative film.
In another instantiation, the present invention's preparation contains the method for moulding that uses in the method for Nano Silver fresh-keeping article and comprises the technology of drenching film and applying, and the plastics of its use comprise PE, PP, PET, OPP, CPP and VMPET.Preferably, the thickness of preservative film is 0.025 millimeter to 0.2 millimeter on the obtained product of the method.
Prepare in the method for the plastic foil that contains Nano Silver in the present invention, the antibiotic particulate that contains Nano Silver can be with the abundant fusion of plastics after extrude or blow out moulding and make film, with generally make finished product earlier after coated with nano silver solution aftertreatment technology thereon is different again, therefore the fresh-keeping article that makes according to method of the present invention has the advantageous characteristic that is better than prior art, promptly, its contained Nano Silver particulate can be uniformly distributed on the plastics, and can expose big surface with abundant contact bacterium, but therefore has the effect of sterilization and deodorizing, and the Nano Silver particulate does not have the phenomenon of migration (migration), can not discharge in plastic foil, be safe for environment therefore.
The fresh-keeping article that the comprises Nano Silver of the present invention scope that is widely used, therefore, another aspect of the present invention also is used to preserve food and agricultural products about the fresh-keeping article that the present invention contains Nano Silver, and it can be effective to prolong edible or fresh-keeping time limit owing to have the effect of sterilization and deodorizing concurrently.In an instantiation, fresh-keeping article of the present invention is to be used for preserving over a long time fresh food materials and food, and is particularly easily corrupt or be difficult for fresh-keeping food materials and food, for example vegetables, fruit, seafood, meat, bread and cake etc.On average, use fresh-keeping article of the present invention can increase about five days to one month freshness date.
With regard to fresh vegetables and fruits, the freshness protection package that contains Nano Silver of the present invention is the most obvious for the fresh-keeping effect of the perishable greengrocery of bottle green, for example green onion, spinach, coriander etc., can have more about one thoughtful one month freshness date compared to general commercially available clip chain bag with same, fresh-keeping effect for other non-bottle green greengrocery takes second place, can increase by about one thoughtful 10 days freshness date, then can increase about 5 to 7 days freshness date for melon-fruit-like vegetable such as green pepper, cuke etc.In addition, on general fruit fresh-keeping, general commercially available freshness protection package was only had an appointment 4 days for the fresh-keeping effect of wax-apple, but the Nano Silver freshness protection package that contains of the present invention then can increase freshness date to 10 day.In addition, for on fish fresh-keeping, salmon for example, when preserving down for 4 ℃, promptly produce obvious stink and oppress inelastic situation at the 3rd day compared to general commercially available clip chain bag with same, the Nano Silver freshness protection package that contains of the present invention obvious stink occurs and oppresses just following the string preservation the 10th talent, therefore can increase about 7 days freshness date.
In addition, the Nano Silver freshness protection package that contains of the present invention has fungistatic effect for fish spoilage organisms (as Staphylococcus aureus), promptly has compared to the about 10 times fungistatic effect of commercially available freshness protection package at the 3rd day.
On the other hand, the fresh-keeping article that contains Nano Silver of the present invention also can be used for preserving agricultural products, for example fresh flower and other plant etc.In an instantiation, the PE film that contains Nano Silver of the present invention can be applicable on fresh flower (comprising cut-flower and potted flower) fresh-keeping.The PE film that contains Nano Silver of the present invention can be made the bagging with up big and down small opening with nonwoven fabric, and wherein flower is to place top opening and end stem then to be positioned at the below.When fresh flower of the present inventionly contains Nano Silver bagging when packing with this; the PE film that contains Nano Silver is to place the place ahead, and bactericidal nano-silver is early withered to avoid fresh flower, and the situation of fresh flower can be known and sees; nonwoven fabric then is positioned at the bagging rear, is not collided and damages with the protection fresh flower.Therefore, preservative film of the present invention can be used for delaying the degradation phenomena of the slotting cut-flower of bottle, comprises that spray is vertical soft, flower comes off and scape is rotted, to prolong the slotting life-span of its bottle.
In other one side, the fresh-keeping article that contains Nano Silver of the present invention can be applicable to the water disinfecting of culture fishery.In an instantiation, the PE preservative film that contains Nano Silver of the present invention can be used for reducing disease and pest to improve the survival rate of aquiculture animal.
Description of drawings
Fig. 1 places nanometer freshness protection package of the present invention and commercially available general freshness seal bag to refrigerate the fresh-keeping result of the test of a couple of days after 4 ℃ for fresh salmon sample.
Fig. 2 places nanometer freshness protection package of the present invention and commercially available general freshness seal bag to refrigerate the fresh-keeping result of the test of a couple of days after 4 ℃ for fresh cake sample.
Fig. 3 places nanometer freshness protection package of the present invention and commercially available general freshness seal bag to refrigerate the fresh-keeping result of the test of a couple of days after 4 ℃ for fresh spinach sample.
Fig. 4 places nanometer freshness protection package of the present invention and commercially available general freshness seal bag to refrigerate the fresh-keeping result of the test of a couple of days after 4 ℃ for fresh wax-apple sample.
Nanometer freshness protection package of the present invention and commercially available general freshness seal bag refrigerate the deodorizing test result of a couple of days after 4 ℃ to Fig. 5 for the fresh fish sample places.
Fig. 6 places nanometer freshness protection package of the present invention and commercially available general freshness seal bag to refrigerate the deodorizing test result of a couple of days after 4 ℃ for fresh crab pin sample.
Fig. 7 is nanometer freshness protection package of the present invention and the commercially available general freshness seal bag curve map for the fungistatic effect of yellow croaker spoilage organisms.
Fig. 8 is nanometer freshness protection package of the present invention and the commercially available general freshness seal bag curve map for the fungistatic effect of cod.
The specific embodiment
The following example is in order to technology contents of the present invention and attainable effect to be described, but non-in order to restriction the present invention.All according to any impartial the variation and modification that the present invention did, all belong to the category of claim of the present invention.
Embodiment
The antibiotic particulate (NAG) that contains Nano Silver among the present invention is according to the preparation of the method described in No. the 6379712nd, the United States Patent (USP).Contain in the preparation of the freshness protection package of antibiotic particulate of Nano Silver or film at this, use the evenly fusion of 0.4 weight % Nano Silver particulate and PE plastics (18 tons/day),, brought into play Nano Silver the effect of bactericidal nano-silver so that can evenly distributing on the PE plastics, and then make film via the technology that blows out moulding (use blown film extruder and under 150-220 ℃ temperature, carry out), about 0.02 millimeter to 0.15 millimeter of its thickness, and according to required specification and folding and be cut into bag, promptly become freshness protection package of the present invention.
Embodiment 1
The fresh-keeping test of Nano Silver freshness protection package
This embodiment is the preservativity of test Nano Silver freshness protection package of the present invention for salmon, cake, spinach and wax-apple, is carried out 10 days sensory evaluation by 5 volunteers.The freshness protection package of control group is available from commercially available sealing bag.
For carrying out the fresh-keeping test of human sense organ, will place nanometer freshness protection package of the present invention and commercially available general freshness seal bag available from fresh salmon, cake, spinach and the wax-apple sample in supermarket respectively, and be stored in 4 ℃ of refrigerators.Then, five volunteers are respectively at the variation with perusal salmon and cake sample in the 1st, 3,5,8 and 10 day, and respectively at the variation of observing spinach and wax-apple sample on the the 1st, 4,7 and 10 day, and the record result, as shown in following table 1 to 4 and Fig. 1 to 4.
Table 1, to the test of the preservativity of fish
Table 2, to the test of the preservativity of cake
Table 3, to the test of the preservativity of spinach
Table 4, to the test of the preservativity of wax-apple
This result shows that Nano Silver freshness protection package of the present invention was better than control group for the preservativity of salmon in the 3rd day on elasticity, promptly, control group frowziness in the time of the 3rd day produces and oppresses more nonelastic, stink produces and Nano Silver freshness protection package of the present invention just began to have slightly in the time of the 5th day, heavier stink occurs up to the 10th talent, control group then has extremely heavy stench flavor to produce (as table 1 and Fig. 1).In addition, on cake is fresh-keeping, nanometer freshness protection package of the present invention elasticity later in the 5th day and control group is that some elementary errors is different, promptly, the cake elasticity Nano Silver freshness protection package more of the present invention of control group is poor in the time of the 8th day, and the cake of control group is organized not softlyer during by the 10th day, but the cake of Nano Silver freshness protection package of the present invention is organized and then still kept soft state (as table 2 and Fig. 2).
On the preservativity of spinach, the stem of spinach did not all have rotten situation generation with control group in the nanometer freshness protection package of the present invention in 10 days, but the blade of control group produced the situation that part is rotted in the time of the 7th day, and the situation that the blade of experimental group just had part to rot in the time of the 10th day produces (as table 3 and Fig. 3).In addition, Nano Silver freshness protection package of the present invention was variant generation for the preservativity of wax-apple in the 4th day, that is, one of two wax-apples of experimental group has fraction to addle, and situation produces but two wax-apples of control group then all addle.In the time of the 7th day, another wax-apple situation that also begins to addle produces in the experimental group, but neitherly surpasses 1/3 of surface, but wax-apple part that addles has surpassed 1/3 of surface in the control group.During by the 10th day, the area that addles of two wax-apples has surpassed surface 1/3 all in the control group, and the part that addles of wax-apple surface 1/3 (as table 4 and Fig. 4) approximately only in the experimental group.
Embodiment 2
The deodorizing test of Nano Silver freshness protection package
This embodiment is the deodorizing capability of test Nano Silver freshness protection package of the present invention for fish and crab pin, is carried out 10 days sensory evaluation by 5 volunteers.The freshness protection package of control group is available from commercially available sealing bag.
For carrying out human sense organ deodorizing test, will place nanometer freshness protection package of the present invention and commercially available general freshness seal bag respectively available from the fresh fish and the crab pin sample in supermarket, and be stored in 4 ℃ of refrigerators.Then, five volunteers were respectively at the variation with the perusal sample in the 1st, 3,5,8 and 10 day, and the record result, shown in following table 5 and Fig. 5 and 6.
Table 5, to the test of the deodorizing capability of fish and crab pin
This result shows the deodorizing capability of Nano Silver freshness protection package of the present invention for fish and crab pin, is that some elementary errors is different in the 1st day with control group, control group has obvious stink to produce in the time of the 3rd day, and Nano Silver freshness protection package of the present invention just had stink (as Fig. 5 and 6) slightly in the time of the 5th day.
The nanometer freshness protection package is to the bacteriostatic test of fish
A. to the fungistatic effect of yellow croaker spoilage organisms
The test bacterial classification of bacteriostasis test is staphylococcus aureus (Staphylococcus aureus, ATCC 25923).With this inoculation in the TSA culture medium that contains 5% sheep blood.Get the bacterium colony of staphylococcus aureus, the concentration of allotment bacterium amount is to McFarland 0.5 (about 1.5 * 10
8The CFU/ milliliter) after, carry out a series of 10 times of serial dilutions with Mueller-Hinton Broth, (promptly Qi Wang bacterial population is 1.5 * 10 till diluting 10000 times
4The CFU/ milliliter).
Place nanometer freshness protection package of the present invention and commercially available general freshness seal bag by get 50 gram samples respectively available from the yellow croaker in supermarket, and add 450 milliliters phosphate buffer solution, after mixing with homogenizer (stomacher), add respectively 1 milliliter 1.5 * 10
4CFU/ milliliter staphylococcus aureus.The 0th day contained total aerobic plate count is to record by being inoculated on the TGEA culture medium in the test yellow croaker sample.
The sample of inoculating Staphylococcus aureus is placed 4 ℃ of refrigerators, and in the time of the 3rd, 5 and 7 day respectively according to total aerobic plate count of said method test sample, and relatively whether Nano Silver freshness protection package of the present invention and general commercially available sealing bag have the ability of bacteria growing inhibiting.
Bacteriostatic test result such as following table 6 and shown in Figure 7.
Table 6, to the test of the bacteriostasis of yellow croaker spoilage organisms
This result shows that the starter bacteria amount of experimental group and control group all is about 7.0 * 10
3The CFU/ milliliter, in the time of the 3rd day, total aerobic plate count of experimental group and control group is respectively 3.3 * 10 in experiment
3And 4.1 * 10
3CFU/ milliliter, the bacterium of experimental group amount be compared to low about 10 times of control group, but total aerobic plate count of experimental group and control group is reduced to 8.5 * 10 respectively in the time of the 5th day
2And 1.0 * 10
3The CFU/ milliliter, the bacterium of experimental group and control group amount then all raises in the time of the 7th day.Therefore, compared to general commercially available freshness protection package, Nano Silver freshness protection package of the present invention had better fungistatic effect for yellow croaker spoilage organisms (Staphylococcus aureus) in 5 days.
B. to the fungistatic effect of cod
In this bacteriostasis test, the commercially available codfish of 200 grams places nanometer freshness protection package of the present invention and commercially available general freshness seal bag respectively, places 5 ℃ of storages, and measures contained aerobic plate count in the flesh of fish at the 0th, 3,5 and 7 day.The mensuration of microorganism is to get the 25 gram flesh of fish to add 225 gram sterilized waters, through after homogenizing, gets 1 milliliter of suitable serial dilutions and places 3M film reagent (3M Petrililm
TM) on, cultivated 48 hours down at 37 ℃, and calculate clump count.
Bacteriostatic test result such as following table 7 and shown in Figure 8.
Table 7, to the test of the bacteriostasis of cod
This result shows, the starter bacteria amount of experimental group and control group and all be about 5.1 * 10 on the 3rd day
4CFU/ gram and 4.4 * 10
4The CFU/ gram.In the time of the 5th day, the aerobic plate count of experimental group is 4.7 * 10 in experiment
4The CFU/ gram, but control group increases to 7.2 * 10
5CFU/ gram, so the aerobic plate count of experimental group is compared to low about 15 times of control group, total aerobic plate count of experimental group and control group is respectively 6.6 * 10 in the time of the 7th day
5And 8.2 * 10
4The CFU/ milliliter, the fungistatic effect that shows experimental group is still than the control group height.Therefore, compared to general commercially available freshness protection package, Nano Silver freshness protection package of the present invention in 7 days for cod in bacterium still have better fungistatic effect.
Embodiment 4
Silver dissolution test (silver migration test)
This test is to measure whether institute's argentiferous can be from wherein discharging in the Nano Silver freshness protection package of the present invention.Standard method of analysis (NIEA W306.52A) according to Environmental Protection Department of TaiWan, China Executive Yuan bulletin; the water of 95 ℃ of temperature is poured in the Nano Silver freshness protection package of the present invention; after three hours, detect silver content in the water with Atomic Absorption Spectrometer; obtain detected value MDL=0.06; it is starkly lower than detectable limit; show in the Nano Silver freshness protection package of the present invention that contained Nano Silver can not disengage or because of the heat stripping, therefore Nano Silver freshness protection package of the present invention is safe for environment in the PE film.
Claims (35)
1. fresh-keeping article, it comprises the antibiotic particulate (NAG) that contains Nano Silver, and it is that amount with 0.1 to 0.8 weight % is present in the plastics with its fusion, and the wherein wt ratio is to be benchmark with plastics weight.
2. according to the fresh-keeping article of claim 1, wherein this contains on the stem marrow (stalkmarrow) that nano-Ag particles contained in the antibiotic particulate of Nano Silver firmly and fifty-fifty is attached to rush (Juncus effuses L.).
3. according to the fresh-keeping article of claim 2, wherein this nano-Ag particles respectively has the argent core that oxidized silver surrounds.
4. according to the fresh-keeping article of claim 1, wherein the diameter of this nano-Ag particles is about 1 to 100 nanometer.
5. according to the fresh-keeping article of claim 1, wherein this antibiotic particulate that contains Nano Silver is by the method manufacturing that comprises the following steps: (1) is cut into several piece with the stem marrow of rush; (2) the stem marrow with stripping and slicing is soaked in the solution that contains nano-Ag particles, so that nano-Ag particles is attached on the stem marrow of stripping and slicing; (3) after adhering to, according to circumstances with hot water and cold water washing stem marrow; (4) will adhere to the stem marrow drying of Nano Silver; And the stem marrow that (5) will contain nano-Ag particles grinds to form appropriate size to make this Nano Silver particulate.
6. according to the fresh-keeping article of claim 1, wherein this antibiotic particulate that contains Nano Silver is that amount with 0.4 weight % is present in the plastics.
7. according to the fresh-keeping article of claim 1, wherein be selected from ABS, PE, PP, PS, PET, PC, DPP, reach CPP with these plastics that contain the antibiotic particulate fusion of Nano Silver.
8. according to the fresh-keeping article of claim 7, wherein these plastics are PE.
9. according to the fresh-keeping article of claim 1, it is selected from preservative film, freshness protection package and crisper.
10. according to the fresh-keeping article of claim 9, wherein this preservative film or freshness protection package are to make by antibiotic particulate and the fusion of PE plastics that 0.1 to 0.8 weight % is contained Nano Silver.
11. according to the fresh-keeping article of claim 10, wherein this preservative film or freshness protection package are to make by antibiotic particulate and the fusion of PE plastics that 0.4 weight % is contained Nano Silver.
12. according to the fresh-keeping article of claim 9, wherein this antibiotic particulate and the even fusion gained of PE plastics admixture that contains Nano Silver is to make preservative film via the technology that blows out moulding.
13. according to the fresh-keeping article of claim 12, wherein this preservative film is folding and/or cuts out and make freshness protection package.
14. according to the fresh-keeping article of claim 9, wherein the thickness of this preservative film is about 0.02 millimeter to 0.15 millimeter.
15. a method for preparing the fresh-keeping article that contains Nano Silver, it comprises containing the antibiotic particulate (NAG) and plastics fusion of Nano Silver, on the plastics, makes the fresh-keeping article that contains Nano Silver through method of moulding so that Nano Silver evenly distributes again.
16. according to the method for claim 15, wherein this fresh-keeping article is selected from preservative film, freshness protection package and crisper.
17. according to the method for claim 15, wherein these plastics comprise ABS, PE, PP, PS, PET, PC, DPP, reach CPP.
18. according to the method for claim 16, wherein this fresh-keeping article that comprises Nano Silver is preservative film or freshness protection package.
19. according to the method for claim 18, wherein this preservative film is the antibiotic particulate (NAG) and the even fusion of PE plastics by 0.1 to 0.8 weight % being contained Nano Silver, and the gained admixture is made via the technology that blows out moulding.
20. according to the method for claim 19, wherein the amount with the PE plastics fusion interpolation the antibiotic particulate that contains Nano Silver is 0.4 weight %.
21. according to the method for claim 19, wherein the thickness of prepared preservative film is about 0.02 millimeter to 0.15 millimeter.
22. according to the method for claim 19, wherein this preservative film is according to required specification and folding and/or be cut into bag, to make freshness protection package.
23. according to the method for claim 15, wherein this method of moulding comprises pouring film and attaching process.
24. according to the method for claim 23, use therein plastics comprise PE, PP, PET, OPP, CPP and VMPET.
25. according to the method for claim 23, the thickness of preservative film is 0.025 millimeter to 0.2 millimeter on the wherein obtained product.
26. the fresh-keeping article according to claim 1 is used to preserve the purposes of food or agricultural products.
27. according to the purposes of claim 26, wherein this fresh-keeping article is selected from preservative film, freshness protection package and crisper.
28. according to the purposes of claim 26, wherein this food comprises fresh food materials and food.
29. according to the purposes of claim 28, wherein this food comprises vegetables, fruit, seafood, meat, bread and cake.
30. according to the purposes of claim 26, wherein this fresh-keeping article is the PE preservative film.
31. according to the purposes of claim 26, wherein this agricultural products comprises fresh flower and other plant.
32. according to the purposes of claim 31, wherein this fresh flower comprises cut-flower and potted flower.
33. according to the purposes of claim 30, wherein this PE preservative film can be made the bagging with up big and down small opening with nonwoven fabric, to be used to pack fresh flower.
34. according to the purposes of claim 33, wherein this PE preservative film is to place the place ahead, and bactericidal nano-silver is early withered to avoid fresh flower, nonwoven fabric then is positioned at the bagging rear, is not collided and damages with the protection fresh flower.
35. the fresh-keeping article according to claim 1 is used to increase the purposes of the survival rate of aquiculture animal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100796514A CN101254017A (en) | 2007-03-02 | 2007-03-02 | Fresh-keeping article containing nano silver, method of preparing the same and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100796514A CN101254017A (en) | 2007-03-02 | 2007-03-02 | Fresh-keeping article containing nano silver, method of preparing the same and use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101254017A true CN101254017A (en) | 2008-09-03 |
Family
ID=39889406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100796514A Pending CN101254017A (en) | 2007-03-02 | 2007-03-02 | Fresh-keeping article containing nano silver, method of preparing the same and use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101254017A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731954A (en) * | 2011-04-15 | 2012-10-17 | 烟台百特塑化制品有限公司 | Preparation method of SEBS nano saddle zipper preservative film bag |
| CN104126641B (en) * | 2014-07-18 | 2015-04-08 | 南京农业大学 | High-voltage field plasma and nano photocatalysis synergistic sterilizing and preserving method for fresh meat |
| CN104788785A (en) * | 2015-04-20 | 2015-07-22 | 浙江一帆日用品有限公司 | Environment-friendly antibacterial fresh-keeping bag |
| CN106398092A (en) * | 2016-08-24 | 2017-02-15 | 宁波鸿雁包装材料有限公司 | Packaging winding film |
| CN108384092A (en) * | 2018-02-27 | 2018-08-10 | 合肥帧讯低温科技有限公司 | Safe, antibacterial and environment-friendly fruit and vegetable preservative film and preparation method thereof |
| CN109265802A (en) * | 2018-09-07 | 2019-01-25 | 中国科学院烟台海岸带研究所 | A kind of antimicrobial preservative film for marine product |
| CN109280267A (en) * | 2018-09-07 | 2019-01-29 | 中国科学院烟台海岸带研究所 | A kind of antimicrobial preservative film |
| CN113966747A (en) * | 2021-06-30 | 2022-01-25 | 中国热带农业科学院环境与植物保护研究所 | Nano-silver composite bacillus agent for preventing and treating syzygium samarangense fruit rot and anthracnose of syzygium samarangense fruit and preparation method thereof |
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2007
- 2007-03-02 CN CNA2007100796514A patent/CN101254017A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731954A (en) * | 2011-04-15 | 2012-10-17 | 烟台百特塑化制品有限公司 | Preparation method of SEBS nano saddle zipper preservative film bag |
| CN104126641B (en) * | 2014-07-18 | 2015-04-08 | 南京农业大学 | High-voltage field plasma and nano photocatalysis synergistic sterilizing and preserving method for fresh meat |
| CN104788785A (en) * | 2015-04-20 | 2015-07-22 | 浙江一帆日用品有限公司 | Environment-friendly antibacterial fresh-keeping bag |
| CN106398092A (en) * | 2016-08-24 | 2017-02-15 | 宁波鸿雁包装材料有限公司 | Packaging winding film |
| CN106398092B (en) * | 2016-08-24 | 2018-10-26 | 宁波鸿雁包装材料有限公司 | A kind of packaging winding film |
| CN108384092A (en) * | 2018-02-27 | 2018-08-10 | 合肥帧讯低温科技有限公司 | Safe, antibacterial and environment-friendly fruit and vegetable preservative film and preparation method thereof |
| CN109265802A (en) * | 2018-09-07 | 2019-01-25 | 中国科学院烟台海岸带研究所 | A kind of antimicrobial preservative film for marine product |
| CN109280267A (en) * | 2018-09-07 | 2019-01-29 | 中国科学院烟台海岸带研究所 | A kind of antimicrobial preservative film |
| CN113966747A (en) * | 2021-06-30 | 2022-01-25 | 中国热带农业科学院环境与植物保护研究所 | Nano-silver composite bacillus agent for preventing and treating syzygium samarangense fruit rot and anthracnose of syzygium samarangense fruit and preparation method thereof |
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