CN101245339B - Alcohol yeast nourishing complex enzyme and uses thereof - Google Patents
Alcohol yeast nourishing complex enzyme and uses thereof Download PDFInfo
- Publication number
- CN101245339B CN101245339B CN2007100553616A CN200710055361A CN101245339B CN 101245339 B CN101245339 B CN 101245339B CN 2007100553616 A CN2007100553616 A CN 2007100553616A CN 200710055361 A CN200710055361 A CN 200710055361A CN 101245339 B CN101245339 B CN 101245339B
- Authority
- CN
- China
- Prior art keywords
- yeast
- enzyme
- alcohol
- parts
- complex enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a multi-enzyme preparation complex formula, which can replace a nutrient salt of a yeast and a special glycated nutrient complex enzyme for alcohol and fuel ethanol, the cultured yeast is healthy and strong, the yeast cells are more, the enzyme activity is strong, at the same time, the yeast has long logarithm growth period and can inhibit the growth of the hybrid bacteria and reduce acidity; the fermentation time is shortened; and the complex formula can further increase the fermentable sugar, improve the utilization ratio of the raw materials, improve alcohol degree and bring considerable economic benefits for enterprises. The invention relates to the alcohol yeast nutrient complex enzyme, the composition is as follows according to the parts by weight: 2 to 3 parts of protease, 1 to 2 parts of phytase, 1 to 2 parts of amylase, 2 to 3 parts of cellulose enzyme and 1 to 2 parts of amylopectin enzyme. When in use, only 0.5 per mill (W/W) of the raw materials need to be scattered into the fermented liquid of a yeast wine culture tank during the alcohol production for preparation, so the production process is simple.
Description
Technical field
The present invention relates to bioengineering field, belong to the food fermentation technology, a kind of specifically distillery yeast nourishing complex enzyme and application thereof.
Background technology
At present, in the Alcohol Production process, dual-enzymatic process is generally adopted in liquefaction, the saccharification of raw material (corn, wheat, Chinese sorghum), and method is simple, effective, and fermentation mainly is a thick mash fermentation.In order to improve raw material availability and to shorten fermentation time, mainly be to add nutritive salt and aspartic protease etc., though fermenting speed is very fast at fermentation stage, but assorted bacterium is many in the mash, the acidity ratio is the height of Ensure Liquid salt not, and acidity higher position loss wine part causes raw material availability to reduce; Have is exactly to add alcohol and the special-purpose saccharification nourishing complex enzyme of alcohol fuel in the saccharification stage again, though it is fast that yeast plays ferment, thalline is bigger, but, the healthy and strong degree of yeast culture and enzymic activity are desirable not enough, not amylolytic not thorough in the raw material, and the Mierocrystalline cellulose in the raw material can not get fine utilization, also has addition excessive (raw material 1 ‰), so can not bring good economic benefits to enterprise.
Summary of the invention:
Purpose of the present invention overcomes the deficiency of above-mentioned technology exactly, the enough stalwartnesses that provide a kind of distillery yeast to cultivate, and yeast can form the obvious growth advantage, suppresses assorted bacterium ideal; Starch in the raw material, dextrin and Mierocrystalline cellulose etc. utilize alcohol yeast nourishing complex enzyme and application thereof completely.
Purpose of the present invention realizes by following scheme:
This alcohol yeast nourishing complex enzyme is made of by ratio of weight and the number of copies following several enzymes:
Proteolytic enzyme 2-3 part
Phytase 1-2 part
Amylase 1-2 part
Cellulase 2-3 part
Starch debranching enzyme 1-2 part
With the raw material of said ratio sterilization in advance, with auger various raw materials are transported to mixing tank and mix, the automatic vaccum packager packing, stochastic sampling detects, and packaging and warehousing promptly gets the Powdered alcohol yeast nourishing complex enzyme formulation products of the present invention.
Described amylase is fungal alpha-amylase.
The complex body that described cellulase is made up of cellulase, beta-glucanase, zytase.
Described proteolytic enzyme is neutral protease.
The using method of above-mentioned alcohol yeast nourishing complex enzyme is: the distillery yeast cultivation stage in biomass to alcohol conversion process drops into 0.5 ‰ of weight by raw material and directly is sprinkled into to the mash of distiller's yeast culture tank.
The special-purpose saccharification nourishing complex enzyme of alternative nutritive salt of zymin of the present invention and alcohol and alcohol fuel suppresses assorted bacterium, reduces acidity, shortens fermentation time;
The present invention can increase fermentable sugars, improves raw material availability, improves alcoholic strength.
Various enzymes have following distinguishing feature from principle analysis:
1, an amount of proteolytic enzyme and phytase can provide nutritive substances such as sufficient nitrogenous source and phosphorus for yeast, make the yeast stalwartness, and yeast cell is more, and percentage of germination improves, and shorten the fermentation time of alcohol, improve usage ratio of equipment, save the spending of water, electricity etc.
2, an amount of cellulase can improve the hydrolysis that utilizes degree and non-starch carbohydrate of starch, can also reduce viscosity, improves the distillation plant utilization ratio.
3, an amount of amylase and Starch debranching enzyme can the halfway jelling starch of hydrolysis and saccharification, amylose starch and amylopectin, increase fermentable sugars.
Embodiment
1, the technological process of production of the present invention: raw material enters factory, and the mensuration enzyme is alive earlier, and whether bacterial detection exceeds standard.Produce 1 ton of alcohol yeast nourishing complex enzyme, the raw material that is up to the standards enters each dog-house by following weight: proteolytic enzyme 250kg; Phytase 150kg; Amylase 200kg; Cellulase 300kg; Starch debranching enzyme 100kg; Production plant is sterilization in advance, and with auger various raw materials are transported to mixing tank and mix, the automatic vaccum packager packing, stochastic sampling detects, packaging and warehousing.
2,---(d≤1.8mm)---high temperature resistant α-Dian Fenmei+backflow is pickled with grains or in wine, and---it is that a part directly entered fermentor tank after 4.0-4.5+ added saccharification enzyme glycolysis (not adding the special-purpose saccharification nourishing complex enzyme of alcohol and alcohol fuel) 40min that boiling liquefaction---flash cooling---sulfuric acid is transferred the mash pH value to using method of the present invention: raw material selection by winnowing in liquid+process water stirring to pulverize (input material quantity); Alcohol distillation that another part enters into the distiller's yeast culture tank, by 0.5 ‰ (w/w) of raw material prozyme directly is sprinkled into the distiller's yeast jar---mash flows into ferment tank (not adding aspartic protease and nutritive salt in addition)---.
3, feed intake 15 tons by raw material per hour and calculate, so per hour need add 7.5kg alcohol yeast nourishing complex enzyme (be in the Alcohol Production raw material 0.5 ‰) to the mash of distiller's yeast culture tank.(compound enzymic preparation of the present invention is Powdered, need only directly be sprinkled into by addition to get final product, and operation sequence is simple.)
4, prozyme of the present invention adds in Alcohol Production and the contrast experiment who does not add:
The common process index of Alcohol Production process:
Grinding particle size: d≤1.8mm material-water ratio: 1: 2.2-2.5
Mash pol: 21.5-22.5BX spice: the poor liquid spice of 30% backflow
Liquefaction temperature: 90-104 ℃ liquefying time: 60min
Saccharification temperature: 58-62 ℃ saccharification time: 50min
The yeast bud ratio: 19-27% yeast number: 3.3-5.1 hundred million
Fermenting-ripening wine with dregs acidity: AC＜5.8 alcoholic strengths: 11.6-12.3v/v
Experimental result such as following table:
The distiller's yeast jar
Remarks: distiller's yeast is a cultured continuously, and incubation time is 8 hours, and the yeast percentage of germination is meant maximum value
Fermentor tank
3, experiment conclusion:
1) the present invention can increase fermentable sugars, and it is obvious to improve alcoholic strength, has improved utilization ratio of raw materials, can bring considerable economic to enterprise.
2) use yeast stalwartness of the present invention, yeast quantity is many, can suppress assorted bacterium, reduces mash acidity.
3) the present invention can reduce mash concentration, improves raw material availability, shortens fermentation time, can be spendings such as enterprise's conserve water, electricity.
Claims (2)
1. alcohol yeast nourishing complex enzyme, it is characterized in that: this 1 ton of alcohol yeast nourishing complex enzyme is made of by weight following several enzymes:
Proteolytic enzyme is 250Kg, phytase 150Kg, amylase 200Kg, cellulase 300Kg and Starch debranching enzyme 100Kg;
Wherein said amylase is fungal alpha-amylase, and described cellulase is that a kind of prozyme is made up of cellulase, beta-glucanase, zytase, and described proteolytic enzyme is neutral protease;
With the raw material of said ratio sterilization in advance, with auger various raw materials are transported to mixing tank and mix, the automatic vaccum packager packing, stochastic sampling detects, and packaging and warehousing promptly gets Powdered alcohol yeast nourishing complex enzyme formulation products.
2. the application of a kind of alcohol yeast nourishing complex enzyme according to claim 1 is characterized in that: the distillery yeast cultivation stage in biomass to alcohol conversion process drops into 0.5 ‰ of weight by raw material and directly is sprinkled into to the mash of distiller's yeast culture tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100553616A CN101245339B (en) | 2007-02-15 | 2007-02-15 | Alcohol yeast nourishing complex enzyme and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100553616A CN101245339B (en) | 2007-02-15 | 2007-02-15 | Alcohol yeast nourishing complex enzyme and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101245339A CN101245339A (en) | 2008-08-20 |
| CN101245339B true CN101245339B (en) | 2011-04-06 |
Family
ID=39946019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100553616A Expired - Fee Related CN101245339B (en) | 2007-02-15 | 2007-02-15 | Alcohol yeast nourishing complex enzyme and uses thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101245339B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105524772A (en) * | 2014-09-29 | 2016-04-27 | 湖南新鸿鹰生物工程有限公司 | Compound ethanol enzyme with acid proteases and method for preparing compound ethanol enzyme |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004097178A (en) * | 2002-09-06 | 2004-04-02 | Yukimasa Kawamura | Method for producing soy sauce-flavored seasoning and product of the same |
-
2007
- 2007-02-15 CN CN2007100553616A patent/CN101245339B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004097178A (en) * | 2002-09-06 | 2004-04-02 | Yukimasa Kawamura | Method for producing soy sauce-flavored seasoning and product of the same |
Non-Patent Citations (7)
| Title |
|---|
| 7行. |
| 7行.;余有贵等.酶制剂在啤酒酿造中的应用.酿酒31卷 5期.2004,31卷(5期),46-50页. * |
| 余有贵等.酶制剂在啤酒酿造中的应用.酿酒31卷 5期.2004,31卷(5期),46-50页. |
| 刘念等.复合酶技术在白酒发酵中的应用与展望.酿酒科技 12期.2006,(12期),65-67页. |
| 刘念等.复合酶技术在白酒发酵中的应用与展望.酿酒科技 12期.2006,(12期),65-67页. * |
| 张强等.发酵增强剂在酒精生产中的应用.酿酒科技 2期.2004,(2期),54页，第1栏，6-9行，13-14行 |
| 张强等.发酵增强剂在酒精生产中的应用.酿酒科技 2期.2004,(2期),54页,第1栏,6-9行,13-14行 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101245339A (en) | 2008-08-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Appiah-Nkansah et al. | Ethanol production from mixtures of sweet sorghum juice and sorghum starch using very high gravity fermentation with urea supplementation | |
| CN101195836B (en) | Novel technique for producing manioc waste ethanol | |
| CN102210383A (en) | Method for preparing mycoprotein feed from waste water and waste residues in processing process of potatoes | |
| CN101555497A (en) | Preparation method of citric acid fermentation solution | |
| CN101698817B (en) | Method for producing white wine by distilled grain continuous solid state fermentation | |
| CN101041849A (en) | Method for producing fuel ethanol by using potato as raw material | |
| Phrueksawan et al. | Direct fermentation of L (+)-lactic acid from cassava pulp by solid state culture of Rhizopus oryzae | |
| CN100342022C (en) | Method for improving alcohol yield fermented from starch material | |
| CN105420217A (en) | Production method and application of high-efficient cellulase mixture | |
| CN101831416B (en) | Pullulanase and production method thereof | |
| CN111040982B (en) | Saccharomyces cerevisiae promoter and preparation method and application thereof | |
| CN1318582C (en) | Once aspergillus niger fermentation process of obtaining complex enzyme of several kinds of enzyme and with high enzyme activity | |
| Germec et al. | Effect of pH control and aeration on inulinase production from sugarbeet molasses in a bench-scale bioreactor | |
| Shen et al. | Application of low-cost algal nitrogen source feeding in fuel ethanol production using high gravity sweet potato medium | |
| CN102860413A (en) | Nutritive feed and preparation method thereof | |
| CN102533570B (en) | Aspergillus niger, application of Aspergillus niger and method for preparing citric acid by fermentation | |
| CN1329504C (en) | A strain for producing composite enzyme and process for producing compoiste emzyme using said strain fermentation | |
| CN100513562C (en) | Preparation method of hybrid hydrolase formulation for promoting methane yield from fermentation | |
| CN101245339B (en) | Alcohol yeast nourishing complex enzyme and uses thereof | |
| CN103392920A (en) | Fermentation method of soybean hulls | |
| CN1224701C (en) | High-effective aciduric liquifying saccharifyig enzyme, its preparation method and application | |
| CN105586367A (en) | Method for conducting fermentative production of citric acid by adding saccharifying enzyme stage by stage based on pH responses | |
| CN102732576B (en) | Method for co-production of biodiesel and biobutanol with lignocellulose as raw material | |
| CN102443611A (en) | Production method of citric acid | |
| CN101948875A (en) | Method for preparing ethanol by low-temperature digestion and thick mash fermentation of starch as raw material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110406 Termination date: 20150215 |
|
| EXPY | Termination of patent right or utility model |