CN101236171A - Enzymic electrode method quantitative ethanol test strip - Google Patents
Enzymic electrode method quantitative ethanol test strip Download PDFInfo
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- CN101236171A CN101236171A CNA2007100370579A CN200710037057A CN101236171A CN 101236171 A CN101236171 A CN 101236171A CN A2007100370579 A CNA2007100370579 A CN A2007100370579A CN 200710037057 A CN200710037057 A CN 200710037057A CN 101236171 A CN101236171 A CN 101236171A
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Abstract
An enzyme electrode quantitative alcohol test strip belongs to the biosensor field. An electrode and an electric circuit are printed on the basal plate of the test paper, a lead wire connected with a readout instrument is printed on one end of the basal plate, the electrode is a porous layer which is printed on a basal plate conductive layer, and biological enzyme, medium and protective agent are fixed on the electrode. The invention adopts the enzyme catalysis oxidation reduction reaction and electrochemical principle and prepares the test paper through printing. As long as the test paper provided by the invention touches test solution, a quantitative test result can be visually and conveniently read out on a corresponding matching testing apparatus. The enzyme electrode quantitative alcohol test strip with simple preparation method facilitates large-scale production.
Description
Affiliated technical field
What the present invention relates to is a kind of enzymic electrode method quantitative test-strips, and particularly a kind of ethanol test strip belongs to the biosensor technique field.
Background technology
At present, the ethanol content test products that occurs on the market adopts the method for designing of alcohol fuel cell mostly, utilizes the relation between concentration of alcohol and the fuel cell output result, judges concentration of alcohol.The major defect of the method is that ethanol test instrument cost of manufacture is higher, uses repeatedly to have hygienic issues, is unfavorable for on-the-spot concentration of alcohol and the large tracts of land popularization of judging fast, and hygienic issues makes the experimenter produce resentment easily.
Summary of the invention
The objective of the invention is to overcome the deficiency in the existing product, a kind of enzyme electrode method ethanol quantitative test bar be provided, make this test paper in a single day contact human body fluid after, can be on the corresponding supporting testing tool convenient test result of reading intuitively.Test paper is disposable products, promptly abandons after the use, does not have hygienic issues.
The present invention is achieved by the following technical solutions, and test paper of the present invention comprises: a substrate; Be printed on electrode on the substrate; Be printed on electric loop on the substrate, so that with the continuous ethanol test of finishing of testing tool; End at substrate is printed on the lead that connects with the readout meter epiphase.Electrode is to be imprinted on the porous layer that forms on the substrate conducting coating; be fixed with the biology enzyme of catalysis ethanol reaction on the electrode, as alcohol oxidase, and a kind of medium that can between enzymic catalytic reaction center and electrode, carry out electron transport; as the potassium ferricyanide and protective agent; as sucrose, so constitute a circuit loop, during reaction according to test solution; as saliva; in the height of concentration of alcohol, produce the current signal of corresponding size, and tested instrument detects and reads.
Working electrode is a kind of double-deck conducting film that is fixed on the holder, at the enzyme that fixedly comprises the catalysis biochemical reaction on the conducting film and the medium that can carry out electron transport between enzymic catalytic reaction is with electrode.This working electrode can transmit the electric current that produces behind the enzymic catalytic reaction.Working electrode and contrast electrode are close, and two electrodes constitute an effecting reaction zone, after connecting with tester, form effective body fluid components test macro.
The present invention adopts bio-sensing and electrochemical principle, makes above-mentioned test paper by fixing method.
The present invention has substantive distinguishing features and marked improvement, and the present invention will be fixed for printing the conducting wire, only plays the conducting effect, makes requirement thereby reduced technology; Material and facility needn't specially be processed, and can obtain by commercially available channel, and cost is very low; Relatively more responsive element such as enzyme are separated from material with protective agent, be fixed on the electrode support substrate.After in a single day test paper of the present invention contacts test solution, can conveniently on corresponding supporting testing tool read test result intuitively, method for making is simple, the precision height, and cost is low, and visual result is convenient, helps field quick detection.
Description of drawings
The 1-substrate; The 2-electrode; The 3-lead; The 4-conducting film.
The present invention is further described below in conjunction with drawings and Examples.
Accompanying drawing is a test paper structural representation of the present invention.
Embodiment
As shown in the figure; test paper of the present invention comprises: a substrate (1); be printed on pair of electrodes (2) on the substrate (1); substrate is printed on electric loop on (1); end at substrate (1) is printed on the lead (3) that connects with the readout meter epiphase; it is characterized in that; electrode (2) is to be imprinted on the porous layer that forms on substrate (1) conductive silver layer; be fixed with the biology enzyme of redox reaction in the catalysis test solution on the electrode (2); as alcohol oxidase; and a kind of medium and protective agent that can between enzymic catalytic reaction center and electrode, carry out electron transport; so constitute a circuit loop, according to the height of concentration of substrate in the test solution, produce the current signal of corresponding size during reaction; and the realization quantitative measurment is read in tested instrument detection.Tested material is an ethanol, and the enzyme of use is an alcohol oxidase.Working electrode (2) is a kind of double-deck conducting film (4) that is fixed on the holder, is fixed with enzyme that comprises the catalysis biochemical reaction and the medium that can carry out electron transport between enzymic catalytic reaction is with electrode on conducting film (4).Electrode (2) constitutes an effecting reaction zone, after connecting with tester, forms effective body fluid components test macro.
Content in conjunction with the inventive method provides down embodiment, and the present invention is further illustrated.
Embodiment: polyester sheet, it is standby to be cut into 80 * 300 millimeters substrates in advance.
(1) add 15% Carboxymethyl Cellulose, 20% the electronic conduction carrier material potassium ferricyanide and thinning agent in conductive carbon ink, mixing is standby;
(2) will have electrode template is installed on the fixing machine standby;
(3) be that the alcohol oxidase of every milliliter of 3000 international unit is allocated in the phosphoric acid buffer wave of every liter of 120 mM with activity, add the Carboxymethyl Cellulose of 600 milligrams every liter of the potassium ferricyanides and 15% simultaneously, it is standby fully to shelve 4 degrees centigrade of refrigerators after the dissolving.
On the said fixing machine, add conductive paste and carry out the fixing of conducting wire; Dry back is adding above-mentioned hybrid conductive carbon ink on the fixing machine automatically, and the silver-colored line of ink marker road of before having printed is covered, and makes electrode.The electrode of having printed need be held in 60 degrees centigrade of baking ovens and solidify 30 minutes, forms 50 pairs of electrodes on each substrate, takes out standby then.When making can be tested the enzyme test peper of ethanol, take out Manufactured basic electrode, the mixed liquor in above-mentioned (3) is added in the holding bottle of two dimension point film instrument,, evenly be fixed on the electrode of making then and set the position by the computer installation parameter.With shearer the test pieces of drying is cut into 40 millimeters long, the test paper finished product of 10 mm wides.
With the test-strips of making, place tester and apply 300 millivolts of operating voltage for two sections at working electrode and contrast electrode, measure Different concentrations of alcohol solution respectively, the result shows that reaction took place after 25 seconds, show and make good test accuracy and the correlativity of electrode, related coefficient reaches 0.9, and the relative standard deviation of test-strips is less than 10%.
Claims (9)
1. enzymic electrode method quantitative ethanol test strip; mainly comprise: a substrate (1); be printed on pair of electrodes (2) on the substrate (1); substrate is printed on electric loop on (1); be printed on the lead (4) of contact instrument at an end of substrate (1); it is characterized in that; electrode (2) is to be imprinted on the reaction zone that forms on substrate (1) conductive layer; be fixed with the biology enzyme of redox reaction in the catalysis test solution on the electrode (2); and a kind of medium and protective agent that can between enzymic catalytic reaction center and electrode, carry out electron transport; so constitute a circuit loop; during reaction according to the height of concentration of substrate in the test solution; produce the current signal of corresponding size, and the realization quantitative measurment is read in the detection of tested instrument.
2. enzymic electrode method quantitative ethanol test strip according to claim 1 is characterized in that, electrode (2) constitutes an effecting reaction zone, after connecting with tester, forms effective liquid component test macro.
3. enzymic electrode method quantitative ethanol test strip according to claim 1 is characterized in that, biology enzyme is a kind of or its potpourri in alcohol oxidase, the alcohol dehydrogenase.
4. enzymic electrode method quantitative ethanol test strip according to claim 1, it is characterized in that the reaction zone constituent material is a kind of or its potpourri in carbon dust, mica powder, conductive titanium dioxide, conduction barium sulphate, electrically conductive polyaniline, conductive carbon fibre, conduction silver powder, silver-coated copper powder, conductive glass powder, conduction nickel powder or the conductive copper powder.
5. enzymic electrode method quantitative ethanol test strip according to claim 1 is characterized in that, protective agent is a kind of or its potpourri in sucrose, trehalose, cellulose, fructose or the maltose.
6. enzymic electrode method quantitative ethanol test strip according to claim 1 is characterized in that, the medium of electron transport is a kind of or its potpourri in potassium ferrocyanide, the potassium ferricyanide, benzoquinones or the nicotinamide adenine dinucleotide.
7. enzymic electrode method quantitative ethanol test strip according to claim 1 is characterized in that, the fixing means of enzyme can be a kind of in printing, spraying, self assembly or the electrochemical polymerization.
8. enzymic electrode method quantitative ethanol test strip according to claim 1 is characterized in that and can become other enzymes and reagent to the biology enzyme of redox reaction in the last catalysis sample of fixing of electrode (2), makes the enzymic electrode method quantitative test-strips of different purposes.
9. enzymic electrode method quantitative ethanol test strip according to claim 1 is characterized in that, test solution is a kind of in saliva, serum, venous blood or the capillary blood.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100370579A CN101236171A (en) | 2007-02-01 | 2007-02-01 | Enzymic electrode method quantitative ethanol test strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100370579A CN101236171A (en) | 2007-02-01 | 2007-02-01 | Enzymic electrode method quantitative ethanol test strip |
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| CN101236171A true CN101236171A (en) | 2008-08-06 |
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| CNA2007100370579A Pending CN101236171A (en) | 2007-02-01 | 2007-02-01 | Enzymic electrode method quantitative ethanol test strip |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232987A (en) * | 2013-04-27 | 2013-08-07 | 西南交通大学 | Preparation method of immobilized enzyme nanofiber membrane for detecting alcohol content in spit |
| CN104198546A (en) * | 2014-08-05 | 2014-12-10 | 绍兴文理学院 | Saliva alcohol concentration detector |
| CN106104264A (en) * | 2014-01-21 | 2016-11-09 | 加利福尼亚大学董事会 | Saliva biosensor and biological fuel cell |
| WO2022056978A1 (en) * | 2020-09-17 | 2022-03-24 | 南京工业大学 | Method for preparing nadh and ethanol biosensing chip |
| CN114441612A (en) * | 2021-12-30 | 2022-05-06 | 广州市赛特检测有限公司 | Electrochemical biosensor, detection method and application |
-
2007
- 2007-02-01 CN CNA2007100370579A patent/CN101236171A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232987A (en) * | 2013-04-27 | 2013-08-07 | 西南交通大学 | Preparation method of immobilized enzyme nanofiber membrane for detecting alcohol content in spit |
| CN103232987B (en) * | 2013-04-27 | 2015-02-11 | 西南交通大学 | Preparation method of immobilized enzyme nanofiber membrane for detecting alcohol content in saliva |
| CN106104264A (en) * | 2014-01-21 | 2016-11-09 | 加利福尼亚大学董事会 | Saliva biosensor and biological fuel cell |
| CN106104264B (en) * | 2014-01-21 | 2021-01-26 | 加利福尼亚大学董事会 | Saliva biosensor and biofuel cell |
| CN104198546A (en) * | 2014-08-05 | 2014-12-10 | 绍兴文理学院 | Saliva alcohol concentration detector |
| WO2022056978A1 (en) * | 2020-09-17 | 2022-03-24 | 南京工业大学 | Method for preparing nadh and ethanol biosensing chip |
| CN114441612A (en) * | 2021-12-30 | 2022-05-06 | 广州市赛特检测有限公司 | Electrochemical biosensor, detection method and application |
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Open date: 20080806 |