CN101230318B - Saccharomyces cerevisiae strain and method of use in preparation of nutrition powder thereof - Google Patents
Saccharomyces cerevisiae strain and method of use in preparation of nutrition powder thereof Download PDFInfo
- Publication number
- CN101230318B CN101230318B CN200710063104A CN200710063104A CN101230318B CN 101230318 B CN101230318 B CN 101230318B CN 200710063104 A CN200710063104 A CN 200710063104A CN 200710063104 A CN200710063104 A CN 200710063104A CN 101230318 B CN101230318 B CN 101230318B
- Authority
- CN
- China
- Prior art keywords
- interpolation form
- iron
- zinc
- powder
- yeast strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000843 powder Substances 0.000 title claims abstract description 25
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 7
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title claims description 15
- 238000002360 preparation method Methods 0.000 title claims description 5
- 235000016709 nutrition Nutrition 0.000 title abstract description 3
- 230000035764 nutrition Effects 0.000 title abstract description 3
- 235000013305 food Nutrition 0.000 claims abstract description 6
- 230000000050 nutritive effect Effects 0.000 claims description 18
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 16
- 239000008247 solid mixture Substances 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 229910052742 iron Inorganic materials 0.000 claims description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 6
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 6
- 239000011575 calcium Substances 0.000 claims description 6
- 229910052791 calcium Inorganic materials 0.000 claims description 6
- 150000001720 carbohydrates Chemical class 0.000 claims description 6
- 235000014633 carbohydrates Nutrition 0.000 claims description 6
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000011777 magnesium Substances 0.000 claims description 6
- 229910052749 magnesium Inorganic materials 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 239000011701 zinc Substances 0.000 claims description 6
- 229910052725 zinc Inorganic materials 0.000 claims description 6
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 4
- 235000013339 cereals Nutrition 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000011669 selenium Substances 0.000 claims description 4
- 229910052711 selenium Inorganic materials 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 3
- JVXHQHGWBAHSSF-UHFFFAOYSA-L 2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate;hydron;iron(2+) Chemical compound [H+].[H+].[Fe+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O JVXHQHGWBAHSSF-UHFFFAOYSA-L 0.000 claims description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 3
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 3
- 241000532030 Menesia Species 0.000 claims description 3
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 3
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 3
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 3
- 229960000304 folic acid Drugs 0.000 claims description 3
- 235000019152 folic acid Nutrition 0.000 claims description 3
- 239000011724 folic acid Substances 0.000 claims description 3
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 3
- 229910052748 manganese Inorganic materials 0.000 claims description 3
- 239000011572 manganese Substances 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- 235000001968 nicotinic acid Nutrition 0.000 claims description 3
- 239000011664 nicotinic acid Substances 0.000 claims description 3
- 235000019155 vitamin A Nutrition 0.000 claims description 3
- 239000011719 vitamin A Substances 0.000 claims description 3
- 229940045997 vitamin a Drugs 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 2
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- NEEHYRZPVYRGPP-IYEMJOQQSA-L calcium gluconate Chemical compound [Ca+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O NEEHYRZPVYRGPP-IYEMJOQQSA-L 0.000 claims description 2
- 235000013312 flour Nutrition 0.000 claims description 2
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 2
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 2
- 239000001095 magnesium carbonate Substances 0.000 claims description 2
- 235000014380 magnesium carbonate Nutrition 0.000 claims description 2
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical group [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 claims description 2
- 229960001708 magnesium carbonate Drugs 0.000 claims description 2
- 229910000021 magnesium carbonate Inorganic materials 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 235000012054 meals Nutrition 0.000 claims description 2
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 2
- 235000015320 potassium carbonate Nutrition 0.000 claims description 2
- 235000019192 riboflavin Nutrition 0.000 claims description 2
- 229960002477 riboflavin Drugs 0.000 claims description 2
- 239000002151 riboflavin Substances 0.000 claims description 2
- 229940100486 rice starch Drugs 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 210000000582 semen Anatomy 0.000 claims description 2
- 239000011670 zinc gluconate Substances 0.000 claims description 2
- 235000011478 zinc gluconate Nutrition 0.000 claims description 2
- 229960000306 zinc gluconate Drugs 0.000 claims description 2
- 235000008935 nutritious Nutrition 0.000 abstract description 7
- 240000007594 Oryza sativa Species 0.000 abstract description 2
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract description 2
- 230000007774 longterm Effects 0.000 abstract description 2
- 230000010355 oscillation Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 235000009566 rice Nutrition 0.000 abstract description 2
- 238000005728 strengthening Methods 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract 1
- 239000002778 food additive Substances 0.000 abstract 1
- 235000013373 food additive Nutrition 0.000 abstract 1
- 235000021049 nutrient content Nutrition 0.000 abstract 1
- 208000037063 Thinness Diseases 0.000 description 5
- 208000007502 anemia Diseases 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 206010048828 underweight Diseases 0.000 description 5
- 229940091258 selenium supplement Drugs 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- XMWCXZJXESXBBY-UHFFFAOYSA-L manganese(ii) carbonate Chemical compound [Mn+2].[O-]C([O-])=O XMWCXZJXESXBBY-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Abstract
The invention provides a vintage yeast strain CGMCC No.1903, and the strain is obtained from fermenting dough, and is bred by monospore separating method, under high acid value condition that PH valueis 2-4, after strong electromagnetic oscillation action, and under the repeatedly alternate actions of zero DEG C and 40 DEG C temperatures. The invention also provides a method which uses the yeaststrain to prepare nutritious powder. The produced nutritious powder contains comprehensive nutrient contents, can be eaten separately as nutritious food, as well as can be added to rice, steamed breadand dish as the food additive. Long-term consumption of the yeast strain plays a positive role on improving the nutrition level, strengthening the immunity and promoting the body of child to growth.
Description
Technical field
The present invention relates to a kind of Wine brewing yeast strain and the application in dietetic product thereof.
Background technology
The nutritious prod kind is a feast for the eyes in the market, but the nutritive substance of one or more needed by human body mostly only is provided, and is unfavorable for the overall equilbrium and the absorption of various materials in the human body.Along with improving constantly of people's living standard, the edible nourishing composition of nutritious prod is had higher requirement.
Yeast is that human practice is used a quasi-microorganism early, is rich in the necessary multiple nutritional components of human body, is VITAMIN and proteinic good source, and rich in protein can be provided, nucleic acid, and VITAMIN and plurality of enzymes, and have no side effect.How seed selection high-quality nutritious yeast bacterial classification and the exploitation that is applied to nutritive food are the focuses of research at present.
Summary of the invention
Technical problem to be solved by this invention provides a kind of yeast strain that can significantly improve food nutrition and health-care effect.
Wine brewing yeast strain Saccharomyces cerevisiae provided by the present invention, be deposited on December 29th, 2006 and be positioned at the No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) of Institute of Microorganism, Academia Sinica, deposit number is CGMCC No.1903.
This bacterial strain obtains from bread dough, adopts the monospore partition method, under the high acidity condition of pH2～4, seed selection obtains under forceful electric power magnetic oscillation effect and the alternating action repeatedly 0 ℃ of temperature and 40 ℃.
The present invention also provides the method that Wine brewing yeast strain CGMCC No.1903 is used to prepare nutritive powder, comprising:
(1) use liquid nutrient medium to cultivate bacterial strain CGMCC No.1903;
(2) cultured mash and the grain dry powder weight ratio according to 1: 2 is mixed, obtain screen underflow behind the crushing screening;
(3) preparation solid mixture, per 1000000 weight parts of described solid mixture contain the composition of following weight part:
Calcium 100000～163100 xitix 75000～98300
Potassium 35000～50800 magnesium 1000～1600
Iron 1000～1666 zinc 900～1432
Riboflavin 150～250 food fibres 100～1000
Vitamin A 80～136 VitB1s 50～83.4
Nicotinic acid 45～88.4 vitamin-Es 30～50
Manganese 25～41 folic acid 13～20.3
Selenium 2～3 vitamins Ds 0.5～0.8
All the other are carbohydrate;
(4) screen underflow and solid mixture are got nutritive powder according to 1: 99 weight ratio mixing.
Described liquid nutrient medium can be wort or potato culture.
Described grain dry powder can be flour, wheat-flour or Semen Maydis powder.
The interpolation form of described calcium can be lime carbonate, calcium chloride or calglucon; The interpolation form of potassium can be salt of wormwood or Repone K; The interpolation form of magnesium can be magnesiumcarbonate, magnesium chloride or Menesia; The interpolation form of iron can be iron(ic) chloride, ferric sulfate or EDTA iron; The interpolation form of zinc can be zinc sulfate or Zinc Gluconate.
The interpolation form of described carbohydrate can be starch, rice meal or wheat-flour.
Wine brewing yeast strain CGMCC No.1903 of the present invention obtains through seed selection under the condition of anthropomorphic dummy's living environment, is a kind of human evolution's of following fungal component.Contain comparatively comprehensively nutritive ingredient by its nutritive powder that makes, it is edible separately to can be used as nutritious prod, also can be used as foodstuff additive and add in rice, steamed bun, the dish, long-term edible for improving trophic level, strengthening immunity, the promotion children effect preferably that grown in stature.
Embodiment
The cultivation of embodiment 1:CGMCC No.1903 bacterial strain
With preserving the lyophilize tube-surface sterilization of bacterial strain, open an end, inject 10 ° of BX malt juice liquid mediums, cultivate activation 48 hours down at 25 ℃.Yeast to be seen is inoculated on the wort agar inclined-plane after sprouting and growing, and cultivates 2～3 days down at 25 ℃～30 ℃, only is activated to the prosperous growth of bacterial classification so repeatedly.Described inclined-plane is synthesized by Fructus Hordei Germinatus extract, yeast extract, peptone, glucose, agar.The bacterial classification of prosperity is moved to liquid tube, little triangular flask, big triangular flask cultivation, in big triangular flask, improve substratum concentration to 12 ° BX.And then order enters Pasteur's jar, Ka Shi jar, the living jar of the Chinese, yeast starter culture tank, breeding jar cultivation.Need pass to sterile air in jar, in will crossing to " logarithmic phase ", cultured mash be gone out jar.
Embodiment 2: the preparation of nutritive powder
(1) with cultured mash among the embodiment 1 and wheat dry powder according to 1: 2 ratio mixing, low temperature or normal temperature are dry rapidly down, pulverize, and cross 80 mesh sieves, obtain screen underflow.
(2) preparation solid mixture, per 1000000 weight parts of described solid mixture contain the composition of following weight part: calcium 150000, xitix 80000, potassium 45000, magnesium 1200, iron 1300, zinc 1000, Riboflavin Tetrabutyrate 00, food fibre 500, vitamin A 100, VitB1 65, nicotinic acid 70, vitamin-E 40, manganese 30, folic acid 18, selenium 2, vitamins D 0.6, all the other are carbohydrate.The interpolation form of calcium, potassium, magnesium, iron, zinc, manganese, selenium is respectively lime carbonate, Repone K, Menesia, EDTA iron, zinc sulfate, manganous carbonate, Sodium Selenite, and carbohydrate adds with the starch form.
(3) gained solid mixture in gained screen underflow in (1) and (2) is promptly made nutritive powder according to 1: 99 weight ratio mixing.
Embodiment 3: the effect analysis of nutritive powder
For understanding the actual effect of nutritive powder of the present invention, 10 kindergartens (1 of Xicheng District, 6 of Chaoyang Districts, 3 of Huairou Districts) have been chosen in Beijing as edible group, continuously edible nutritive powder of the present invention from 2002 to children's Physique growth and healthy state.And choose and eat nutritive powder condition of the present invention similar (board expenses and living condition are approaching in each district, child-care center's male and female children constitutes roughly the same, age composition is roughly the same), but the kindergarten of not edible nutritive powder of the present invention totally 10 in contrast group compare research.In March, 2006, Beijing healthcare hospital for women ﹠ children preschool child care Administration and Registration Division was added up the continuous 3 years physical examination data of nearly 15000 children in these kindergartens.
Statistical standard:
Malnutritive judgement criteria: the reference crowd standard value of employing is the international standard that NCHS formulates, and deducts 2 standard deviations with median and (2SD) is underfed criterion
Under-weight: the age complicated variant heavily is lower than judgement criteria
Hypoevolutism: the age, other height was lower than judgement criteria
Become thin: the height complicated variant heavily is lower than judgement criteria
Fat: the height complicated variant heavy more than or equal to median add 2 standard deviations (+2SD)
Comparable number: the number of measurement of comparison numerical value 2 year same period is arranged, should reject the number of W/H 〉=M+2SD
Increase: the number that height, body weight all increase
It is qualified to increase: 5 centimetres of growth in stature, the number that body weight gain is 1.6 kilograms
Anaemia: oxyphorase is lower than 11g/dl
Statistical method:
Use EpiData and carry out the data input, Spss carries out statistical analysis, adopts X
2Check.
Statistics:
Edible 1 year test group of table 1 children and control group analysis
| Edible group | Control group | X 2 | P | |
| Sum | 3418 | 2785 | ||
| Under-weight (%) | 5(0.15) | 4(0.14) | 1.000 | |
| (%) becomes thin | 2(0.06) | 3(0.11) | 0.663 | |
| Hypoevolutism (%) | 4(0.12) | 6(0.20) | 0.360 | |
| Fat (%) | 208(6.09) | 150(5.39) | 1.53 | 0.216 |
| Comparable sum | 1919 | 1509 | ||
| Increase (%) | 1912(99.64) | 1508(99.93) | 0.086 | |
| Increase qualified (%) | 1810(94.32) | 1359(90.06) | 21.95 | 0.000 |
| Sum | 3368 | 2440 | ||
| Anaemia (%) | 4(0.12) | 8(0.33) | 3.00 | 0.083 |
Edible 2 years test group of table 2 children and control group analysis
| Edible group | Control group | X 2 | P | |
| Sum | 2404 | 1825 | ||
| Under-weight (%) | 1(0.04) | 6(0.33) | 0.047 |
| Edible group | Control group | X 2 | P | |
| (%) becomes thin | 0(0.00) | 1(0.05) | 0.432 | |
| Hypoevolutism (%) | 4(0.17) | 5(0.27) | 0.512 | |
| Fat (%) | 201(8.36) | 153(8.38) | 0.00 | 0.979 |
| Comparable sum | 1819 | 1175 | ||
| Increase (%) | 1811(99.56) | 1135(96.60) | 39.77 | 0.000 |
| Increase qualified (%) | 1722(94.67) | 1033(87.91) | 44.31 | 0.000 |
| Sum | 2404 | 1792 | ||
| Anaemia (%) | 2(0.08) | 2(0.11) | 1.000 |
Edible 3 years test group of table 3 children and control group analysis
| Edible group | Control group | X 2 | P | |
| Sum | 534 | 386 | ||
| Under-weight (%) | 0(0.00) | 0(0.00) | ||
| (%) becomes thin | 0(0.00) | 0(0.00) | ||
| Hypoevolutism (%) | 0(0.00) | 0(0.00) | ||
| Fat (%) | 43(8.05) | 38(9.84) | 0.90 | 0.344 |
| Comparable sum | 454 | 239 | ||
| Increase (%) | 454(100.00) | 237(99.16) | 0.119 | |
| Increase qualified (%) | 444(97.80) | 218(91.21) | 15.88 | 0.000 |
| Sum | 534 | 383 | ||
| Anaemia (%) | 0(0.00) | 0(0.00) |
By the result who eats group and control group as can be seen: edible 1 year height and weight increases qualified X
2=21.95, the P value is 0.000, and height and weight increased X in edible 2 years
2=39.77, the P value is 0.000, increases qualified X
2=44.31, the P value is 0.000, and edible 3 years height and weights increase qualified X
2=15.88, the P value is 0.000, and P value＜0.05 shows that there are significant difference in edible group and control group.Other project such as under-weight, become thin, hypoevolutism, obesity, anaemia P value 〉=0.05, show and do not see notable difference.Analyze experimental result as can be seen, nutritive powder of the present invention makes that children grow in stature, weight increase, but obese person's minimizing, this explanation nutritive powder of the present invention is promoting aspect the growth of children's physique positive effect is being arranged.
Claims (2)
1. Wine brewing yeast strain Saccharomyces cerevisiae, its preserving number is: CGMCC No.1903.
2. a Wine brewing yeast strain as claimed in claim 1 is used to prepare the method for nutritive powder, comprising:
(1) use liquid nutrient medium to cultivate bacterial strain as claimed in claim 1;
(2) cultured mash and the grain dry powder weight ratio according to 1: 2 is mixed, obtain screen underflow behind the crushing screening;
(3) preparation solid mixture, per 1000000 weight parts of described solid mixture contain the composition of following weight part:
Calcium 100000～163100 xitix 75000～98300
Potassium 35000～50800 magnesium 1000～1600
Iron 1000～1666 zinc 900～1432
Riboflavin 150～250 food fibres 100～1000
Vitamin A 80～136 VitB1s 50～83.4
Nicotinic acid 45～88.4 vitamin-Es 30～50
Manganese 25～41 folic acid 13～20.3
Selenium 2～3 vitamins Ds 0.5～0.8
All the other are carbohydrate;
(4) screen underflow and solid mixture are got nutritive powder according to 1: 99 weight ratio mixing,
Wherein, described liquid nutrient medium is wort or potato culture; Described grain dry powder is flour, wheat-flour or Semen Maydis powder; The interpolation form of described calcium is lime carbonate, calcium chloride or calglucon; The interpolation form of described potassium is salt of wormwood or Repone K; The interpolation form of described magnesium is magnesiumcarbonate, magnesium chloride or Menesia; The interpolation form of described iron is iron(ic) chloride, ferric sulfate or EDTA iron; The interpolation form of described zinc is zinc sulfate or Zinc Gluconate; The interpolation form of described carbohydrate is starch, rice meal or wheat-flour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710063104A CN101230318B (en) | 2007-01-26 | 2007-01-26 | Saccharomyces cerevisiae strain and method of use in preparation of nutrition powder thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710063104A CN101230318B (en) | 2007-01-26 | 2007-01-26 | Saccharomyces cerevisiae strain and method of use in preparation of nutrition powder thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101230318A CN101230318A (en) | 2008-07-30 |
| CN101230318B true CN101230318B (en) | 2010-05-19 |
Family
ID=39897139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710063104A Expired - Fee Related CN101230318B (en) | 2007-01-26 | 2007-01-26 | Saccharomyces cerevisiae strain and method of use in preparation of nutrition powder thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101230318B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357342B (en) * | 2014-11-20 | 2018-03-20 | 新疆大学 | A kind of high-quality yeast of Xinjiang local characteristic crusty pancake dough and its application in system is cramed food into one's mouth |
| CN105055458B (en) * | 2015-06-15 | 2018-10-23 | 山东凤凰生物有限公司 | Prevent the probiotics preparation and preparation method thereof of osteoporosis |
| CN115039887A (en) * | 2022-08-15 | 2022-09-13 | 源民生物科技(山东)有限公司 | Probiotics composition with functions of muscle and bone enhancement |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219368A (en) * | 1997-12-11 | 1999-06-16 | 山西大学 | Glossy ganoderma nourishing powder and its production method |
| CN1636465A (en) * | 2004-12-23 | 2005-07-13 | 贾稜 | Hesha edible baker's saccharomyces nutrient powder |
| CN1666654A (en) * | 2005-05-08 | 2005-09-14 | 孟庆煜 | Preparation method of common house-fly grub freeze-drying nutritious powder |
| CN1875760A (en) * | 2006-06-21 | 2006-12-13 | 林奕群 | Vegetables nutrition powder and preparation method thereof |
-
2007
- 2007-01-26 CN CN200710063104A patent/CN101230318B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219368A (en) * | 1997-12-11 | 1999-06-16 | 山西大学 | Glossy ganoderma nourishing powder and its production method |
| CN1636465A (en) * | 2004-12-23 | 2005-07-13 | 贾稜 | Hesha edible baker's saccharomyces nutrient powder |
| CN1666654A (en) * | 2005-05-08 | 2005-09-14 | 孟庆煜 | Preparation method of common house-fly grub freeze-drying nutritious powder |
| CN1875760A (en) * | 2006-06-21 | 2006-12-13 | 林奕群 | Vegetables nutrition powder and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 贾稜等.8605特种食用面包酵母菌的研究.食品科学25 增刊.2004,25(增刊),11-18. |
| 贾稜等.8605特种食用面包酵母菌的研究.食品科学25 增刊.2004,25(增刊),11-18. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101230318A (en) | 2008-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102687853B (en) | Preparation method of cordyceps militaris bacterial powder and application thereof | |
| CN102907626A (en) | Making method for dehydration tempeh | |
| CN105639639A (en) | Quinoa prepared powder rich in nutrient and preparation method thereof | |
| CN107625125A (en) | A kind of Tiny ecosystem eats ferment and preparation method thereof | |
| CN105494539A (en) | Gluten-free cassava nutritional bread and preparation method thereof | |
| CN105249100A (en) | Production method of fermented fruit and vegetable juice and Chinese rice wine composited function beverage | |
| CN101602999B (en) | Sweet potato vinegar and grade fermentation process for clinker thereof | |
| CN103911244A (en) | Corn kwas fermented beverage and preparation process thereof | |
| CN108029955A (en) | A kind of selenium-rich steamed bun and preparation method thereof | |
| CN102612984B (en) | Method for preparing cordyceps sinensis mycelia with fresh and tender sweet corn | |
| CN103299824B (en) | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn | |
| CN102715363A (en) | Tilapia mossambica iron-rich blended hard particles feed and its preparation method | |
| CN104256286A (en) | Nutritious food with function of boosting immunity and preparation method of nutritious food | |
| CN101230318B (en) | Saccharomyces cerevisiae strain and method of use in preparation of nutrition powder thereof | |
| CN106387652A (en) | Preparation method of ganoderma probiotic fermentation product | |
| CN101647548B (en) | Protogenic state fermenting type burdock-watermelon jam | |
| CN103829190A (en) | Preparation technology of sweet potato pickled vegetable | |
| Bisht et al. | Bamboo shoots for food in North-East India: conventional and contemporary | |
| CN108450705A (en) | A kind of compound microbial culture starter, the biological fermentation feed and preparation method thereof prepared using the leavening | |
| KR101692122B1 (en) | Method for preparing functional dough with fermented material of turmeric and rice bran and snack prepared using same | |
| CN101642244A (en) | Grain-bean type nutritional food formula and prepration process | |
| CN103829247A (en) | Potbelly-conditioning enzymes and preparation method thereof | |
| Asqarov et al. | MAKKAJO’XORI KEPAGI ASOSIDA TAYYORLANADIGAN BIOPARCHALANUVCHAN IDISHLAR KIMYOVIY TARKIBI | |
| KR20180038425A (en) | Product using a method for a natural fermented rice using Kimchi lactic acid bacteria | |
| CN107594523A (en) | A kind of nutrition travel food of shelf-stable and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING CENTURY HESHA HIGH-TECH CO., LTD. Effective date: 20120515 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20120515 Address after: 100037 Beijing city Xicheng District Fucheng gate Baiwanzhuang Street No. 13 No. 2 hospital building 1 room 101 Co-patentee after: Beijing century union Hi Tech Co.,Ltd. Patentee after: Jia Die Address before: 100037 Beijing city Xicheng District Fucheng gate Baiwanzhuang Street No. 13 No. 2 hospital building 1 room 101 Patentee before: Jia Die |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181203 Address after: 100037 Room 101, No. 2 Building, No. 13 Million Village Street, Fuchengmen, Xicheng District, Beijing Patentee after: Jia Jing Address before: 100037 Room 101, No. 2 Building, No. 13 Million Village Street, Fuchengmen, Xicheng District, Beijing Co-patentee before: Beijing century union Hi Tech Co.,Ltd. Patentee before: Jia Jing Effective date of registration: 20181203 Address after: 100037 Room 101, No. 2 Building, No. 13 Million Village Street, Fuchengmen, Xicheng District, Beijing Co-patentee after: Beijing century union Hi Tech Co.,Ltd. Patentee after: Jia Jing Address before: 100037 Room 101, No. 2 Building, No. 13 Million Village Street, Fuchengmen, Xicheng District, Beijing Co-patentee before: Beijing century union Hi Tech Co.,Ltd. Patentee before: Jia Die |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100519 Termination date: 20210126 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |