CN101229530B - Centrifugal separator - Google Patents
Centrifugal separator Download PDFInfo
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- CN101229530B CN101229530B CN2007101666679A CN200710166667A CN101229530B CN 101229530 B CN101229530 B CN 101229530B CN 2007101666679 A CN2007101666679 A CN 2007101666679A CN 200710166667 A CN200710166667 A CN 200710166667A CN 101229530 B CN101229530 B CN 101229530B
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Abstract
A centrifugal separator includes a driving source; a rotor rotatable about a rotation axis by the driving source; and a swing member including an accommodation space for retaining a liquid analyte to be separated, the swing member being pivotally suspended by the rotor; a detecting unit for detecting the number of rotations of the rotor and a determination unit for determining whether or not the center of gravity of the rotor during the rotation is offset from the rotation axis based on data obtained by the detecting unit.
Description
This case be the application number submitted to August 14 calendar year 2001 be 01814323.7 (PCT/JP01/07022), denomination of invention divides an application for the patent application of " centrifugal separating device and the analytical equipment with this device ".
Technical field
The present invention relates to a kind of centrifugal separating device, and, the present invention relates to have the analytical equipment of centrifugal separating device.
Background technology
As everyone knows, in blood, not only contain red blood cell and white blood cell, also have various compositions such as glucose, albumin, calcium.Method as measuring the contained concentration of these compositions has optical means and electrochemical method.Particularly, be to contain carrying of reagent and put on the piece (usually, being processed into rectangular test film) with lighting as the test portion (blood) of detected object.For the special component in the test portion, the reagent meeting demonstrates certain reaction.With optical means or electrochemical method the reaction condition of this moment is analyzed.In optical means, be to use up the coloured moiety of putting on the piece is carried in reagent to shine, then consequent reverberation and transmitted light are analyzed.In the electrochemistry gimmick, carry the electrochemical change of putting in the piece when redox reaction takes place when reagent with electrode analysis.Analyze according to these, calculate the concentration of the special component in the blood.
No matter be optical means or electrochemical method, when the composition (blood plasma) beyond the blood cell composition is carried out concentration determination,, be preferably in advance with blood cell composition and separating plasma for fear of measure error.For this reason, adopt centrifugal separating device usually.
In the past, in order to measure the constituent concentration in the blood automatically, the scheme of the analytical equipment of various built-in centrifugal separating devices had been proposed.What showed in JP-A-61 (1986)-No. 13158 communique is exactly an example wherein.As expression in the application's the accompanying drawing 11, this analytical equipment of being showed (integral body is represented with symbol 8) has centrifugal separating device 9, pipette device 80, thermostat 81 and optical detecting device (not having diagram).
Shown in figure 12, centrifugal separating device 9 has 3 rotating discs 90 that equally spaced are separated from each other on imaginary circumference.These rotating discs 90 are fixed on the rotating shaft 92 through horizontal arm 92.Rotating shaft 92 can be done the batch (-type) rotation with the interval of 120 degree round its axle center.Like this, each rotating disc 90 moves to next halt after just can on any point among three halt A shown in Figure 11, B, the C, pausing.
Shown in figure 12, on each rotating disc 90,, be provided with the several holes 93 that equally spaced separates along their circumference edge.Shown in figure 13, in each hole 93, be provided with the cylindrical container that can place test tube 94 95 that can shake freely.
Shown in figure 11, on analytical equipment 8 82, be provided with and coil the roughly the same opening of 90 diameters 83, make the dish 90 (90a) that is positioned No. 1 halt A above expose.Like this, just can make test tube 94 (Figure 12) be easy to be inserted in the cylindrical container 95 (Figure 13) in the dish 90a.
After in all cylindrical containers 95 of test tube insertion dish 90a,, make dish 90a move to halt B No. 2 through making rotating shaft 91 (Figure 12) rotation 120 degree.On this position, with the rotation of the speed more than the 3000rpm test portion is centrifugalized through making dish 90a.
Then, rotate 120 degree again, make dish 90a move to halt C No. 3 through making rotating shaft 91 (Figure 12).C is corresponding with halt, on analytical equipment 8, on 82, is provided with the pipette patchhole 84 of minor diameter.Through make dish 90a rotation off and on halt C, can make arbitrary the test tube 94 that is placed on the dish 90a can both be placed on pipette patchhole 84 under.From each test tube 94, gather supernatant (blood plasma) through pipette patchhole 84.
The blood plasma of being gathered is lighted the reagent of the test film of preparing at thermostat 81 places (diagram slightly) and is carried and put on the piece through lighting hole 85.Each reagent carries that to put the chromogenic reaction that takes place in the piece be to utilize above-mentioned method of optically measuring to analyze.
Though above-mentioned existing analytical equipment 8 has various characteristics, thereby have suitable function, also have following deficiency.
Generally, being arranged on the number of the test tube 94 on the centrifugal separating device, is not to be restricted according to each centrifugation operation.That is, some occasion, all cylindrical containers 95 may be used to preserve test tube 94 (test portion is arranged), and under other occasion, the quantity of the test tube 94 of placement also might be less than the quantity of cylindrical container 95.Under preceding a kind of situation, the center of gravity of three rotating discs 90 (and test tube 94 that test portion is arranged) is consistent with the axle center of rotating shaft 92.Therefore, rotating shaft 92 is done normal rotation round the axle center.But under latter event, the center of gravity of three rotating discs 90 (and test tube 94 that test portion is arranged) and the axle center of rotating shaft 92 are inconsistent.Therefore, in the middle of operation, the axle runout appears in rotating shaft 92 easily, so the probability that centrifugal separating device 9 breaks down increases.
In the past, in order to prevent this disadvantageous situation, when the number of the test tube 94 that adds test portion is less than allowed maximum, made the rotation of centrifugal separating device 9 obtain the optimum balance state through method for distinguishing with regard to being necessary.Particularly, be necessary be in test tube 94 in each rotating disc be arranged on mutually as far as possible away from the position on, perhaps use model test tube as balanced body.
But,, and then significantly reduced the efficient of assaying owing to such operation bothers very much.Particularly having only one or two doctor's infirmary, using above-mentioned analytical equipment 8 just not talkative very suitable.So, usually, in such small-scale hospital, not once to carry out a large amount of blood tests, necessary when also can be described as operational analysis device 8, must make the rotation of centrifugal separating device 9 obtain the operation of optimum balance state.And, existing analytical equipment 8, too huge for the small-scale hospital that uses it, on the suitable place to place of searching, also bother very much.
Summary of the invention
The present invention obtains after the above-mentioned situation having considered.Therefore, an object of the present invention is to provide a kind of small-sized analytical equipment that constitutes according to can once only analyzing a kind of test portion efficiently.
And another object of the present invention provides a kind of centrifugal separating device that uses this analytical equipment.
According to the 1st aspect of the present invention, a kind of centrifugal separating device with drive source, rotor and rotary body is provided.Rotor is rotated round axis of rotation through above-mentioned drive source.Rotary body movably is being draped with respect to above-mentioned rotor, and is provided with the receiving space of preserving separate object liquid.
Above-mentioned rotor preferably according to as formations of getting off: have with respect to above-mentioned axis of rotation and the center of gravity of off-centre; When remaining at the separate object liquid with scheduled volume when making the rotation of above-mentioned rotor under the state in the above-mentioned receiving space and making above-mentioned rotary body turn to specified angle, the center of gravity of the assembly that is made up of above-mentioned rotor and above-mentioned rotary body is consistent with above-mentioned axis of rotation.
Above-mentioned rotary body is preferably free with respect to above-mentioned rotor loading and unloading.
Preferably, above-mentioned rotary body is provided with a pair of outstanding axial region, and above-mentioned rotor is provided with the fixed part that combines with above-mentioned axial region.
Preferred said fixing parts have a pair of guide part separated from each other, and each guide part has the breach that holds above-mentioned axial region, and the inclined-plane that links to each other with above-mentioned breach.
The umbrella portion that preferred above-mentioned rotary body has upper opening and restrains to upper opening.
Preferred above-mentioned rotary body comprises the container portions of having stipulated above-mentioned receiving space and is installed in the lid on this container portions, and above-mentioned upper opening is formed on the above-mentioned lid, and above-mentioned lid has the certain flange of diameter that links to each other with above-mentioned upper opening.
Preferably, above-mentioned rotor is provided with the brake component that can limit the waving angle of the above-mentioned relatively rotor of above-mentioned rotary body.
Preferred above-mentioned rotary body has under the not rotation status of above-mentioned rotor, the vertical central consistent with the axis of rotation of above-mentioned rotor.
Preferred above-mentioned rotary body has under the not rotation status of above-mentioned rotor, the vertical central that departs from mutually with the axis of rotation of above-mentioned rotor.
Preferred above-mentioned rotor has bottom surface and the side that is provided with breach.
Preferred centrifugal separating device of the present invention also has: detect the checkout gear of the revolution of above-mentioned rotor, and whether the center of gravity when judging the rotation of above-mentioned rotor according to the data that obtained by this checkout gear departs from the decision maker of above-mentioned axis of rotation.
Preferred above-mentioned decision maker is beginning to rotate from above-mentioned rotor through after the stipulated time and the revolution of above-mentioned rotor when not reaching the regulation revolution judges that center of gravity be an off-centre.
Preferred centrifugal separating device of the present invention has in case the center of gravity when judging the revolution of above-mentioned rotor by above-mentioned decision maker when being off-centre, can stop the brake apparatus of above-mentioned rotor rotation.
According to the 2nd aspect of the present invention, a kind of analytical equipment with centrifugal separating device, pipette device, determinator and detent mechanism is provided.Centrifugal separating device comprises the separation container that centers on the axis of rotation rotor rotated and be draped rotationally with respect to this rotor.The pipette device is drawn and is joined the liquid sample in the separation container, and the test portion that this is drawn to is provided in the reagent.Determinator for example according to optical principle, is to use up above-mentioned reagent is shone, and measures its reverberation or transmitted light.Detent mechanism moves on the assigned address above-mentioned rotor after centrifugation finishes.In store predetermined amount of liquid test portion in the above-mentioned separation container.Under this state, when making the rotation of above-mentioned rotor and making above-mentioned separation container rotate to predetermined angular, the center of the assembly that is made up of above-mentioned rotor and above-mentioned separation container is consistent with above-mentioned axis of rotation.
Preferred above-mentioned positioner comprises the brake component that is used to detect the checkout gear that is set in above-mentioned epitrochanterian position point and whether departs from, when above-mentioned position point departs from, makes above-mentioned rotor rotor rotated whirligig, makes driving mechanism that above-mentioned rotor moves and move up and down with the mobile interlock of above-mentioned rotor through this driving mechanism on the direction that the above-mentioned axis of rotation with this rotor intersects.
Preferred analytical equipment of the present invention, the casing that also has sliding stand and on sliding stand, move through above-mentioned driving mechanism.Above-mentioned rotor remains in the above-mentioned casing, and above-mentioned sliding stand has high-order tabular surface and low level tabular surface.Above-mentioned brake component forms according to the mode that moves up and down of joining through mobile and above-mentioned high-order tabular surface or low level tabular surface along with above-mentioned casing.
Preferably on above-mentioned rotor, be provided with fixing hole, through above-mentioned brake component being inserted this fixing hole, the position of fixing above-mentioned rotor.
Preferred said fixing hole should make it be easy to insert above-mentioned brake component, and is taper.
Description of drawings
Fig. 1 representes the stereogram based on the analytical equipment of the embodiment of the invention 1.
Fig. 2 explains the pipette mechanism of the analytical equipment that is used for embodiment 1 and the figure of optical detection device.
Fig. 3 is the figure that the centrifugal separating device of the analytical equipment that is used for embodiment 1 is described.
Fig. 4 is the stereogram of rotor that expression is used for the centrifugal separating device of Fig. 3.
Fig. 5 is the figure that the separation container of the centrifugal separating device that is used for Fig. 3 is described.
Fig. 6 is the figure of state in separation container when rotation of key diagram 5.
Fig. 7 representes the profile based on the analytical equipment of the embodiment of the invention 2.
Fig. 8 A-8C is the definite figure that controls in position that the rotor of the analytical equipment that is used for embodiment 2 is described.
Fig. 9 is the figure that the stopper mechanism of the analytical equipment that is used for embodiment 2 is described.
Figure 10 is the figure of the stopper mechanism effect of key diagram 9.
Figure 11 is the stereogram of the existing analytical equipment of expression.
Figure 12 is the key diagram that is used for the centrifugal separating device of existing analytical equipment.
Figure 13 is the cutaway view that the XII-XII line in Figure 12 obtains.
The specific embodiment
Below, with reference to accompanying drawing, the preferred embodiments of the present invention are at length explained.
Fig. 1～Fig. 6 is the figure that is used to explain the biological sample analytical equipment (its integral body is represented with symbol 1) based on the embodiment of the invention 1.This analytical equipment 1 that is constituted is the concentration that is used for measuring the contained special component of blood (for example glucose, albumin, calcium etc.).As depicted in figs. 1 and 2, analytical equipment 1 has workbench 2, test film mounting table 3, centrifugal separating device 4, pipette device 5 and optical detecting device 6.
As shown in Figure 1, on workbench 2, be provided with the outlet 20 of various action button 2a, display 2b and record-paper K.Action button 2a is used to set test condition, and sets the button of the operation of analytical equipment 1 with this.Display 2b is used to operating result of representing measurement result and action button 2a etc.Record-paper K, for example heat-sensitive paper is used to write down measurement result.
At the leading flank of workbench 2, but be provided with free switch the door 21.When door 21 was closed, test film mounting table 3 was taken in workbench 2 inside with centrifugal separating device 4.When door 21 was opened, as shown in Figure 1, test film mounting table 3 can be pulled out from workbench 2 with centrifugal separating device 4.
Placing test film 7 (with reference to Fig. 2) on the test film mounting table 3.Test film 7 shown in the figure constitutes as measuring multi-component device, on the matrix material 70 of strip, is provided with several (having 5 among Fig. 2) reagents and carries and put piece 71.Carry in each reagent and to put in the piece 71, having soaked into can the reagent of chromogenic reaction takes place in one of them with these detected object materials such as glucose, albumin, calcium.Also can be not as illustrated embodiment, carry and put piece but a reagent only is set on a slice matrix material, so just formed the structure that can measure one-component.Multicomponent is measured and is placed in No. 1 groove 30 of test film mounting table 3 with test film 7.As shown in Figure 1, No. 1 groove 30 is elongated, and is perpendicular with the glide direction of test film mounting table 3.On the other hand, the test film that is used for measuring one-component is placed among in No. 2 grooves 31 (figure has 6) of test film mounting table 3.Each No. 2 grooves 31 are elongated, parallel with the glide direction of test film mounting table 3.
As shown in Figure 3, centrifugal separating device 4 has casing 40, rotor 41, small container 42, checkout gear 80, decision maker 81 and brake apparatus 82.Be described below, small container 42 can load and unload and be mounted freely on the rotor 41.
On casing 40, be provided with through hole 40a, it just below disposing DC engine M1.The diapire 43 of rotor 41 is fixed on the output shaft m of engine M1.Therefore, in case engine M1 running, rotor 41 will be around the axle center rotation of output shaft m.As shown in Figure 1, in the side of through hole 40a, be provided with the sharp mouth placement section 40b that maintenance is installed in the sharp mouth T of (Fig. 2) on the pipette device 5.
As shown in Figure 3, casing 40 can move back and forth sliding stand 24 through well-known drive unit 23.Drive unit 23 is a kind of for example rack rails, pinion gear mechanism.At this moment, on the one hand pinion (not having diagram) is rotatably mounted on the casing 40, on the other hand, the rack rails that will be meshed with pinion (not having diagram) is fixed on the sliding stand 24.The reversible engine M2 of configuration in casing 40, its rotating shaft is connected with above-mentioned pinion.Utilize such structure, when the door 21 of workbench 2 was opening, centrifugal separating device 4 just can automatically be exposed to the outside of workbench 2, perhaps is housed in the inside of workbench 2.
Like Fig. 3 and shown in Figure 4, on the perisporium 44 of rotor 41, be provided with the breach 44b that links to each other with upper opening 44a.And, be provided with protuberance 44c in a relative side with breach 44b.Therefore, the center of gravity of rotor 41 will be partial to protuberance 44c one side just with predetermined extent.When driving engine M1 with fixing revolution, this offset make the small container 42 of having accommodated the blood of confirming amount in advance along with rotor 41 together, in the axle center around rotation reposefully.Therefore, if according to the embodiment shown in the figure, just can not resemble use balanced body the prior art, but only use a small container also can centrifugalize test portion.
On protuberance 44c, be provided with the mark 80a that constitutes checkout gear 80 parts.About checkout gear 80, will make hereinafter and be described in detail.
On rotor 41, on the 44d, be provided with location crown 45.As shown in Figure 4, location crown 45 has 45A of bridge portion and a pair of guide part 45B.The 45A of bridge portion extends to the top of breach 44b, and guide part 45B then disposes clamping upper opening 44a with being separated from each other.Each guide part 45B has 45a of bearing portion and inclined-plane 45b.The 45a of bearing portion is located at the approximate center place of guide part 45B.The lower end of inclined-plane 45b is connecting the 45a of bearing portion.
As shown in Figure 5, small container 42 comprises body 46 and lid 47.Body 46 is a kind of tubular articles that the end is arranged with receiving space 48c (with reference to Fig. 6).Extend a pair of reverse each other arm 48 from the sidewall of body 46.Each arm 48 all has axial region 48a and fixed part 48b.Two axial region 48a have same axle center Ax.The diameter of each axial region 48a is all roughly the same with the width of the 45a of bearing portion of location crown 45, and the diameter of each fixed part 48b big than the 45a of bearing portion all.Arm 48 rotates and freely is placed among the 45a of bearing portion.Like this, small container 42 just can rotate with respect to rotor 41 by Ax round the axle center.
Like Fig. 5 and shown in Figure 6, lid 47 comprises central opening 47a, the 47b of umbrella portion and flange 47c.Opening 47a is connecting the receiving space 48c of body 46.The 47b of umbrella portion converges taper to opening 47a.The diameter of flange 47c is a definite value.Lid 47 has the outstanding joint portion 47d (with reference to Fig. 6) by the 47b of umbrella portion downwards.Through joint portion 47d is embedded among the receiving space 48c, thereby lid 47 is installed on the body 46.
Above-mentioned body 46 and lid 47 are processed through resin forming.Distance from the arm 48 that links to each other with body 46 to the bottom is about 10～20mm, and the volume of receiving space 48c is about 300～500 μ l.Centrifugation operation through following is divided into blood cell component and blood plasma with the blood test portion that is housed in the body 46.At this moment, blood cell component deposition, blood plasma forms supernatant.
As shown in Figure 2, pipette device 5 comprises nozzle 50 and is installed in the sharp mouth T on this nozzle.What do not show among the figure is through the elongated pressure generator tube in nozzle 50 inside, in its inside, to produce negative pressure essential concerning drawing test portion selectively and discharge the essential malleation of test portion.The test portion of being drawn remains in the space in the sharp mouth T, and does not contact with nozzle 50.In the example shown in the figure, pipette device 5 is drawn the separation of supernatant (blood plasma) in the small containers 42, its each reagent of lighting test film 7 is carried put on the piece 71.For this reason, pipette device 5 can move on vertical direction and horizontal direction.
As shown in Figure 2, optical detecting device 6 comprises several light-emitting components 60 and several photo detectors 61.Each light-emitting component 60 is made up of the element as light emitting diode (LED).61 of each photo detectors are made up of the element as photo-electric conversion element, to accept carrying the light of putting piece 71 reflections and coming by corresponding reagent.In illustrated example, there are 5 reagents to carry and put piece 71, corresponding therewith, light-emitting component 60 and photo detector 61 with its equivalent amount are also arranged.But, in fact, in optical detecting device 6, be provided with than this more light-emitting component 60 and photo detector 61.Particularly, as stated, on test film mounting table 3, can place multicomponent simultaneously and measure with test film 7 (have 5 reagents carry put piece 71) and 6 one-component mensuration with test film (have 1 reagent carry put piece 71).Therefore, in optical detecting device 6, put the irradiation that piece 71 can receive light one by one, just be provided with 11 light-emitting components 60 for 11 (=5+1 * 6) reagents are carried.Corresponding therewith, in optical detecting device 6, just be provided with 11 photo detectors 61.These light-emitting components 60 are fixed in the workbench 2 with photo detector 61.
Mark 80a can be with the mode irradiates light different with the side of rotor 41.For example, mark 80a can be the element that has higher or lower reflectivity than the side of rotor 41.For example, mark 80a can apply printing ink through the side at rotor 41 or sticking cloth is realized.Perhaps, also can be through recess or protuberance being set in rotor 41 sides, with them with the 80a that marks.
When the weight of the rotor 41 that is rotating when identification is uneven, just can stop to apply voltage, rotor 41 is stopped the rotation through brake apparatus 82 to rotor 41.Like this, in the time of just can be in small container 42 is not placed on rotor 41, can stop the running of centrifugal separating device 4 automatically.
Then, method for using and the working method to analytical equipment 1 with said structure describes.
During the concentration of the special component (like glucose, albumin, calcium etc.) in measuring blood except that the blood cell component, just must in analytical equipment 1, centrifugalize the blood cell component.Therefore, at first will the blood of gathering (for example 250 μ l) be injected small container 42.Then, small container 42 is configured in the rotor 41, drives engine M1.Like this, small container 42 is done (14000rpm) rotation at a high speed with rotor 41, thereby makes blood cell component and separating plasma.
When the length of small container 42 is 10～20mm; When internal volume is 400～500 μ l; Through the rotation (14000rpm) of small container 42, the centrifugal force that acts on the gas-liquid interface in the container is about 1000G, and the centrifugal force that acts on small container 42 bottoms is more than the 2000G.Suppose that under such condition being suitable for making the rotational time of the small container 42 of blood cell and separating plasma is exactly 10 ± 5 minutes.
Under non-driven state, as shown in Figure 5, small container 42 is suspended in the rotor 41.At this moment, the rotating shaft of the axle center of small container 42 and rotor 41 (with reference to Fig. 2) unanimous on the whole.If rotor 41 in this state because action of centrifugal force, small container 42 will resemble Fig. 3 and Fig. 5 represented round the axle center Ax rotation of axial region 48a.At this moment, if having no barrier, because action of inertia, the rotational angle of small container 42 is maximum to surpass 90 degree.But in fact, owing to exist the 45A of bridge portion at location crown 45 places that are located at rotor 41, therefore can limit the rotational angle of small container 42.In embodiment as shown in the figure, can keep the rotational angle about 80～85 degree always, make small container 42 continue rotation with rotor 41.
Because the rotation of small container 42, can produce air-flow along the profile of lid 47.If this is not taked what relative measures, this strand air-flow just might enter in the small container 42 from opening 47a very much.If air-flow gets in the small container 42, will in small container 42, produce turbulent flow, make that finally near the air pressure the opening 47a reduces.Because this state can cause being kept at the liquid component premature evaporation irrelevantly in the small container 42, is not therefore selected for use.
But, if according to illustrated embodiment, just can prevent the above-described problem from occurring effectively, as stated, lid 47 has the 47b of umbrella portion and the flange 47c with certain diameter that converges taper to opening 47a.Like this, will advance along the path shown in the arrow F among Fig. 6 along the air-flow of the outer surface of lid 47.That is, air-flow will be towards advancing away from the direction of opening 47a.Therefore, just aforesaid turbulent flow can not take place in small container 42, thereby, not only can not make opening 47a inaccessible, can also suppress to be kept at the evaporation of the liquid component in the small container 42.
After the centrifugation of test portion is accomplished, just can automatically carry out the concentration determination of special component.This automatic concentration determination comprise substantially with supernatant (blood plasma) light reagent carry put on the piece 71, optical detection learns that reagent carries the color status of putting piece and calculate these steps of testing result.
Particularly, blood plasma being lighted reagent carries to put on the piece 71 and is done as follows.At first, sharp mouth T is installed on the nozzle 50 of pipette device 5.The sharp mouth T that need are installed is placed on the sharp mouth placement section 40b (with reference to Fig. 1) on the casing 40 of being located at centrifugal separating device 4 in advance.Nozzle 50 is moved to the place, top that is positioned at the sharp mouth T that places, and the right good nozzle 50 that makes again is mobile downwards.Sharp mouth T automatically is installed on the nozzle 50.After installing sharp mouth T, test film shown in Figure 27 is placed on the test film mounting table 3, test film mounting table 3 is housed in the workbench 2.Then, as shown in Figure 2, from small container 42, draw blood plasma with pipette device 5, and the blood plasma of sucking-off lighted to carry in one of them reagent of test film 7 put on the piece 71.Particularly, the top of the small container 42 of pipette device 5 in being suspended on rotor 41 is moved.Then, pipette device 5 is moved downward, thereby the lower end of sharp mouth T is immersed in the blood plasma in the small container 42.Through reducing the air pressure in the nozzle 50 in this state, make to produce negative pressure in the sharp mouth T, thereby draw blood plasma.The air pressure that reduces in the nozzle 50 can be realized through driving the external pump (not having diagram) of joining with nozzle 50.The blood plasma of being drawn is kept among the sharp mouth T.Then, carry out the discharge of this blood plasma.Concrete is at first makes pipette device 5 move to desired reagent to carry and put on the piece 71.Then, use external pump to improve the air pressure in the nozzle 50, and then make the blood plasma that collects be discharged to reagent to carry and put on the piece 71.
Above-mentioned blood plasma absorption discharging operation can carry to put on the piece 71 and carry out repeatedly in each reagent of test film 7.
Then, detect and to learn that reagent carries the color status of putting piece.The reagent that particularly, will demonstrate particular color according to the concentration of the special component in the blood plasma is soaked and is contained in each reagent and carries and put on the piece 71.Carry for these reagents that chromogenic reactions take place and to put piece 71, as stated, carry out illumination one by one with light-emitting component 60 and penetrate, and photo detector 61 is accepted its reverberation one by one.When photo-electric conversion element is used as photo detector, through the electric charge of each photo detector 61 generations with the corresponding output level of its light income (degree promptly develops the color).These electric charges are by calculation part 62 outputs.
Then, according to the electric charge of being exported, carry out the calculation of testing result.Calculation part 62 is by constituting like CPU, ROM and RAM etc.Calculation part 62 is through the program of operation memory in ROM, by the light income of photo detector 61, confirms the concentration of special component.Particularly, ask for relevant slotted line (function) between light income and the specific component concentration in advance.According to this slotted line, just can determine the concentration of special component.
Be presented on the display 2b by resulting in of obtaining of calculation part 62.And, if the action button 2a that sets according to the user operates, perhaps operation automatically, just can will result in is recorded on the recording mediums such as record-paper K and magnetic card in the record portion 63.
Secondly, with reference to Fig. 7～Figure 10, Benq is in the analytical equipment (whole with 1 ' expression) of the embodiment of the invention 2.In addition, in these figure, parts identical with the analytical equipment of explaining hereinbefore with reference to Fig. 1～Fig. 6 or element etc. exist together mutually and adopt same symbol, omit the explanation to these same parts simultaneously.
The analytical equipment 1 ' of embodiment 2 is made up of sliding stand 24 and centrifugal separating device 4 ', and the controlling organization of rotor 41 ' is different with the analytical equipment 1 of embodiment 1.
As shown in Figure 9, on sliding stand 24 ', be provided with pair of guide rails 24A '.This like Fig. 7 and shown in Figure 10, has two tabular surface 24a ' and 24b ' that height is different to guide rail 24A ', and the inclined-plane 24c ' that connects these tabular surfaces.
Shown in Fig. 8 A～8C, on the 44e ' of the side of rotor 41 ', be provided with the mark 44f ' that constitutes by absorptivity height or the high material of light scattering property.And, on the 44e ' of side, be provided with the big fixing hole 43a ' of lower opening.Rotor 41 ' links to each other with DC engine M1.In embodiment 2,, can DC current (voltage) be provided intermittently for DC engine M1 through controlling organization (diagram slightly).Therefore, shown in Fig. 8 A and 8B, rotor 41 ' can be according to each predetermined angle theta rotation.
On rotor 41 ', hanging small container 42.When rotor 41 ' did not rotate, specific range (with reference to Fig. 8 A) was only departed from the axle center of the rotating shaft of rotor 41 ' and small container 42.If it is according to this configuration, when rotor 41 ' rotation, easier when it is more consistent with the rotating shaft of rotor 41 ' than the axle center of adopting small container 42 with on the blood of predefined centrifugal action in small container 42.Therefore, even the revolution of rotor 41 ' is less, also can operate the centrifugation that blood suitably is scheduled to.And,, can reduce driving voltage and can reduce operating cost through reducing the revolution of rotor 41 '.
As stated, the rotating shaft of rotor 41 ' departs from (with reference to Fig. 8 A) mutually with the axle center of small container 42.Under such structure,, when the centrifugation operation is accomplished, probably make the stop position of small container 42 all different at every turn so if do not take any countermeasure.In this case, also just can not carry out suitably blood plasma absorption through pipette device 5 (with reference to Fig. 2).
For fear of this inappropriate situation, the centrifugal separating device 4 ' among the embodiment 2 has detent mechanism 72 ', and according to behind the centrifugation EO, the mode that small container 42 is parked on the precalculated position constitutes.This detent mechanism 72 ' has checkout gear 73 ' and brake component 74 '.
Shown in Fig. 7 and Fig. 8 A～8C, checkout gear 73 ' has light-emitting component 73A ' and photo detector 73B '.For example, light-emitting component 73A ' can be made up of LED, and photo detector 73B ' then is made up of the element with photoelectric converting function.Light-emitting component 73A ' and photo detector 73B ' are relative status on the 44e ' of the side of rotor 41 ', and carry on substrate 73C '.Substrate 73C ' is fixed on the casing 40 '.Light-emitting component 73A ' can carry out illumination and penetrate to the side 44e ' of rotor 41 ', photo detector 73B ' then can accept the light by side 44e ' reflection.When the illumination of being sent by light-emitting component 74B ' is mapped to last time of mark 44f ', because light is absorbed or is scattered on mark 44f ', the light minimizing that photo detector 73B ' upward accepts.By photo detector 73B ' output and the corresponding outputs level signals of light income, be transported to information processing device (diagram slightly).In this information processing device, can certification mark 44f ' the situation in the place ahead through checkout gear 73 ', or certification mark 44f ' is positioned at the situation in the place ahead of checkout gear 73 '.
For the ease of understanding, above-mentioned detection device 73 ' not only can detect the position of rotation of rotor 41 ', can also be used for the revolution of detection rotor.Owing to only just can detect, so help reducing cost to two different physical quantitys with a checkout gear.
As clearly illustrating that among Fig. 7, Fig. 9 and Figure 10, brake component 74 ' comprises the 74A ' of support sector.Bottom at the 74A ' of support sector is being supported that by pair of rotatable rotary body 74B ' polarized plug 74C ' is fixed on the top of the 74A ' of support sector.
As shown in Figure 7, the through hole 49a ' of the bottom surface 49 ' of the part of the 74A ' of support sector through being located at casing 40 ' is stretched out by casing 40 '.
Each rotary body 74B ', along tabular surface 24a ', 24b ' and the inclined-plane 24c ' of guide rail 24A ', one side rotation one side moves.
If according to said structure, when casing 40 ' moved, rotary body 74B ' and guide rail 24A ' joined, and moved with casing 40 '.This when mobile when carrying out, corresponding to the height of guide rail 24A ', the position of polarized plug 74C ' moves up and down.For example, when brake component 74 ' was moved by the right side of Figure 10 to the left, when rotary body 74B ' climbed on inclined-plane 24c ', polarized plug 74C ' then moved up.Along reverse path when descending, when from the inclined-plane during 24c ' landing, polarized plug 74C ' then moves down when in contrast.Like this, polarized plug 74C ' (so that brake component 74 ') just can be along with the mobile of casing 40 ' moves up and down.As shown in Figure 7; When rotary body 74B ' is positioned at lower last time of tabular surface 24b '; The upper end position of polarized plug 74C ' is also lower than the bottom surface of rotor 41 ', and is positioned at higher last time of tabular surface 24a ' as rotary body 74B ', and the upper end position of polarized plug 74C ' is higher than the bottom surface of rotor 41 '.
As shown in Figure 7, when mark 44f ' was positioned at the opposite of subtend face 73c ' of substrate 73C ', the fixing hole 43a ' of rotor 41 ' was arranged to the axle center of polarized plug 74C ' unanimous on the whole.Therefore, if under the axle center of polarized plug 74C ' and fixing hole 43a ' state unanimous on the whole, polarized plug 74C ' is moved upward, will insert among the fixing hole 43a ' locked rotor 41 ' like Fig. 7 and the front end of polarized plug 74C ' that makes shown in Figure 9.At this moment, because the lower opening of fixing hole 43a ' is big, so polarized plug 74C ' is inserted among the fixing hole 43a ' reliably.
If detent mechanism 72 ' according to this configuration after the centrifugation operation is accomplished, just can make mark 44f ' that rotor 41 ' is positioned on the position on substrate 73C ' opposite.Concrete situation is described below.
At first, shown in Fig. 7 and Fig. 8 A, the light that is sent by light-emitting component 73A ' is radiated on the side 44e ' of rotor 41 ', and its reverberation reflexes on the photo detector 73B '.At this moment, if shown in Fig. 7 and Fig. 8 B, when mark 44f ' is not in the opposite of substrate 73C ', pulse voltage will be transported on the DC generator M1, make rotor 41 ' only rotate with angle θ.For example, when the amplitude (pulse time of delivery) of pulse voltage was 20～30 milliseconds of left and right sides, the anglec of rotation of rotor 41 ' was approximately 10 degree.
After making rotor 41 ' rotation, be mapped on the side 44e ' of rotor 41 ' in this illumination that will send by light-emitting component 73A ', its reverberation is reflexed on the photo detector 73B '.At this moment, as shown in Figure 8, if mark 44f ' is positioned at the opposite of substrate 73C ', then the pulsed drive of rotor 41 ' finishes.On the other hand, if with the above ground shown in Fig. 7 and Fig. 8 B, when mark 44f ' is not in the opposite of substrate 73C ', pulse voltage is transported on the DC generator M1, will make rotor 41 ' only rotate with angle θ.Such pulsed drive just can make mark 44f ' back and forth movement until relative with the central portion of substrate 73C '.
In addition, when detecting by the light quantity of light-emitting component 73A ' irradiation and during by the light quantity of photo detector 73B ' reflection, both can be till rotor 41 ' have obtained locating during in carry out continuously, also can carry out off and on.
After making mark 44f ' and substrate 73C ' relatively, ought to keep the rotor 41 ' locking of relative status with brake component 74 '.The locking of rotor 41 ' is to carry out through casing 40 ' is moved by the right side of Fig. 7 and Figure 10 to the left.And the mobile of casing 40 ' carries out (with reference to Fig. 7) through driving mechanism 23.
Like this; When make rotor 41 ' and be stabilized in small container 42 location on the rotor 41 ' after; If as stated with reference to Fig. 2, draw the supernatant in the small containers 42 with pipette device 5, the reagent that this supernatant is lighted test film 7 is carried and is put on the piece 71; Measure reagent through optical detecting device 6 and carry the colour developing degree of putting piece 71, just can confirm the concentration of special component.
Although because the present invention has made explanation hereinbefore, obviously they also should change over other form.This change does not exceed thought of the present invention and scope, and all changes of practitioner of this area ought to be included in the scope of following Patent right requirement certainly.
Claims (13)
1. centrifugal separating device, it is taken in this workbench in the analytical equipment with workbench, it is characterized by, and this centrifugal separating device has:
Drive source;
Center on the rotor that axis of rotation rotates through said drive source;
Be draped rotationally with respect to said rotor and be provided with the rotary body of the receiving space of preserving separate object liquid;
The optical markings that utilization is arranged at said rotor detects the checkout gear of the revolution of said rotor; With
Whether the center of gravity when judging that according to the optical data that is obtained by this checkout gear said rotor rotates departs from said axis of rotation and assert the decision maker of the separate object liquid that in the receiving space of said rotary body, whether keeps an amount of.
2. centrifugal separating device as claimed in claim 1 is characterized by,
Said decision maker is beginning to rotate from said rotor through after the stipulated time and the revolution of said rotor when not reaching the regulation revolution judges that center of gravity be an off-centre.
3. centrifugal separating device as claimed in claim 1 is characterized by,
Have in case the center of gravity when judging the revolution of said rotor by said decision maker when being off-centre, stops the brake apparatus of said rotor rotation.
4. centrifugal separating device as claimed in claim 1 is characterized by,
Said rotary body is free with respect to said rotor loading and unloading.
5. centrifugal separating device as claimed in claim 4 is characterized by,
Said rotary body is provided with a pair of outstanding axial region, on said rotor, is provided with the fixed part that combines with said axial region.
6. centrifugal separating device as claimed in claim 5 is characterized by,
Said fixed part has a pair of guide part separated from each other, and each guide part has the breach that holds said axial region, and the inclined-plane that links to each other with this breach.
7. centrifugal separating device as claimed in claim 1 is characterized by,
The umbrella portion that said rotary body has upper opening and restrains to upper opening.
8. centrifugal separating device as claimed in claim 7 is characterized by,
Said rotary body comprises the container portions of stipulating said receiving space and is installed in the lid on this container portions, and said upper opening is formed on the said lid, and said lid has the certain flange of diameter that links to each other with said upper opening.
9. centrifugal separating device as claimed in claim 1 is characterized by,
Said rotor is provided with the brake component that can limit the waving angle of the said relatively rotor of said rotary body.
10. centrifugal separating device as claimed in claim 1 is characterized by,
Said rotary body has under the state that said rotor does not rotate, the vertical central consistent with the axis of rotation of said rotor.
11. centrifugal separating device as claimed in claim 1 is characterized by,
Said rotary body has under the state that said rotor does not rotate, the vertical central that departs from mutually with the axis of rotation of said rotor.
12. centrifugal separating device as claimed in claim 1 is characterized by,
Said rotor has the bottom surface and side jaggy is set.
13. centrifugal separating device as claimed in claim 1 is characterized by,
Leading flank at said workbench is provided with door, and under this closing state, centrifugal separating device is taken in the said workbench, and under the state that said door is opened, centrifugal separating device can be pulled out from said workbench.
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000248216 | 2000-08-18 | ||
| JP2000248216 | 2000-08-18 | ||
| JP2000248215 | 2000-08-18 | ||
| JP2000-248216 | 2000-08-18 | ||
| JP2000-248215 | 2000-08-18 | ||
| JP2000248215 | 2000-08-18 | ||
| JP200164264 | 2001-03-08 | ||
| JP2001064264 | 2001-03-08 | ||
| JP2001-64264 | 2001-03-08 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN01814323.7A Division CN1250331C (en) | 2000-08-18 | 2001-08-14 | Centrifugal separator and analyzer with separator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101229530A CN101229530A (en) | 2008-07-30 |
| CN101229530B true CN101229530B (en) | 2012-03-14 |
Family
ID=35926698
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100827744A Expired - Lifetime CN100357034C (en) | 2000-08-18 | 2001-08-14 | Centrifugal separator and analyzer with the separator |
| CN2007101666679A Expired - Lifetime CN101229530B (en) | 2000-08-18 | 2001-08-14 | Centrifugal separator |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100827744A Expired - Lifetime CN100357034C (en) | 2000-08-18 | 2001-08-14 | Centrifugal separator and analyzer with the separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN100357034C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107617511B (en) * | 2012-04-30 | 2020-06-12 | 生命技术公司 | Centrifuge device and system |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1213024A (en) * | 1997-09-10 | 1999-04-07 | 三洋电机株式会社 | Centrifugal dewatering device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0239579Y2 (en) * | 1985-03-11 | 1990-10-23 | ||
| US5935051A (en) * | 1997-08-29 | 1999-08-10 | Beckman Instruments, Inc. | Blood separation device |
| JPH11276931A (en) * | 1998-03-30 | 1999-10-12 | Japan Tobacco Inc | Centrifugal separator |
| CN2393550Y (en) * | 1999-09-09 | 2000-08-30 | 浙江博朗生物工程有限公司 | Automatic blood separator |
-
2001
- 2001-08-14 CN CNB2005100827744A patent/CN100357034C/en not_active Expired - Lifetime
- 2001-08-14 CN CN2007101666679A patent/CN101229530B/en not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1213024A (en) * | 1997-09-10 | 1999-04-07 | 三洋电机株式会社 | Centrifugal dewatering device |
Non-Patent Citations (3)
| Title |
|---|
| JP昭61-13158A 1986.01.21 |
| JP昭61-150062U 1986.09.17 |
| JP特开平11-276931A 1999.10.12 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100357034C (en) | 2007-12-26 |
| CN101229530A (en) | 2008-07-30 |
| CN1727072A (en) | 2006-02-01 |
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