CN101225095A - Method for extracting aucubin from pedicularis plants - Google Patents
Method for extracting aucubin from pedicularis plants Download PDFInfo
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- CN101225095A CN101225095A CNA2008100173772A CN200810017377A CN101225095A CN 101225095 A CN101225095 A CN 101225095A CN A2008100173772 A CNA2008100173772 A CN A2008100173772A CN 200810017377 A CN200810017377 A CN 200810017377A CN 101225095 A CN101225095 A CN 101225095A
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- aucubin
- pedicularis
- filtration
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Abstract
The invention discloses a method for extracting aucubin from Pedicularis, which is characterized in that Pedicularis meal is extracted with 8-fold aether by refluxing method for two times, and each time for 1 hour to remove liposoluble impurity; residue of the extraction is extracted with 10-fold methanol by cold-soaking method for two times, and each time for 24h; filtration is respectively conducted, and filtrate is combined; proper amount of methanol is recovered from the filtrate under reduced pressure; 2-fold acetone is added to the hot filtrate, and white floccule is precipitated; filtration is conducted when the floccule is completely precipitated; the floccule after filtration is recrystallized with ethanol-methanol (10:1) for three times, then the white needle-like crystal of aucubin is obtained. The method for extracting aucubin from Pedicularis has the advantages that the original plant is low in cost and rich in source, and the content of aucubin is about 0.90mg/g through HPLC, ensuring abundant materials for the preparation of aucubin and the derivatives; the method is both simple and feasible, and brings certain economic and social benefits for the industrial production of aucubin and the derivatives.
Description
Technical field
The present invention relates to the extraction and separation method of the active ingredient of natural plant of pharmaceutical industry, particularly a kind of method of from pedicularis plants attractive in appearance, extracting aucubin.
Background technology
Aucubin aucubin (having another name called aucubin(e)) is a kind of iridoid glycoside compounds, and molecular formula is C
15H
22O
9, molecular weight is 346.33, mp181 ℃.This compound mainly is present in the medicinal plants such as Caprifoliaceae, Plantaginaceae and Eucommiaceae, and discovery is also arranged in some flowers and fruit.Experimental study shows that aucubin has wide biological activity, for example resisting oxidation and delaying senility (Li Farong, Yang Jianxiong, Shen Xiaoting, Li Shujuan. the antioxidation in vitro research (English) of aucubin. Shaanxi Normal University's journal (natural science edition), 2004,32 (3): 98-101.), anti-inflammatory antivirus action, hepatoprotective effect (Li Changgong, the honor of canal osmanthus. the anti-hepatic-B virus medicine progress of research. research and development of natural products, 2002,14 (6): 81-87.), the hypotensive effect of persistent high efficiency (Zhu Yuan, Wang Yaqin. the progress of aucubin. herbal medicine, 2006,37 (6): 947-949.) etc.
Kind of plant surplus scrophulariaceae lousewort Pedicularis Linn. has 500 approximately originates in the Northern Hemisphere, and majority grows on frigid zone and the high mountain, and this belongs to about three/a kind of China of originating in, and (Chinese Plants will editorial board of the Chinese Academy of Sciences compiles mainly to be distributed in the southwest.Chinese Plants will, 68 (2), Beijing: Science Press, 1963), resource is very abundant.This platymiscium mainly have reinforce vital energy, effect (the new medical college in Jiangsu, Chinese medicine voluminous dictionary (first volume), Shanghai: Science and Technology of Shanghai press, 1977) such as enriching yin and nourishing kidney, invigorating the spleen and replenishing QI, invigorating the spleen and regulating the stomach.According to the literature search that the applicant did, at present, the research emphasis of this platymiscium is the pharmaceutical use of its chemical ingredients and the plain glycosides compound of phenylpropyl alcohol, extracting method also only limits to conventional column chromatography for separation, does not find the relevant report about the technical study of extraction separation aucubin from this platymiscium as yet.
Summary of the invention
The objective of the invention is to, a kind of method of extracting aucubin from pedicularis plants is provided, for the preparation of aucubin and derivative thereof provides abundant raw material and convenient extraction separation method.
To achieve these goals, the present invention adopts following technical solution:
A kind of method of extracting aucubin from pedicularis plants is characterized in that, carries out according to the following steps:
Get Herb of Resupinate Woodbetony meal raw material, extract twice with the aether backflow of 8 times of amounts of raw material, each 1h is with the weeding of grease solubility impurity;
With the methyl alcohol cold soaking extraction twice of 10 times of amounts of raw material, the time is 24h to residue respectively, filters, and amalgamation liquid, reclaim under reduced pressure methyl alcohol add the acetone of 2 times of amounts of raw material while hot to an amount of in filtrate, and visible white flocks is separated out, and waits to precipitate and separates out complete after-filtration;
Throw out usefulness ethanol after the filtration and recrystallizing methanol three times, the wherein ethanol of mixing solutions: methyl alcohol=10: 1, can obtain white needle, identify through thin-layer chromatography, be aucubin.
The used former plant of method of the present invention is with low cost, and aboundresources, and the content of its aucubin is determined at about 0.90mg/g through the HPLC method, and the preparation that can be aucubin and derivative thereof provides abundant raw material; Secondly, this method is simple and feasible, for the industrial production of aucubin and derivative thereof is brought certain economic and social benefit.
Description of drawings
Fig. 1 is the process flow sheet that aucubin extracts, and the figure illustrates the technical process of extracting aucubin from the lousewort plant.
Fig. 2 is the thin-layer chromatogram of aucubin.
The present invention is described in further detail below in conjunction with embodiment that the contriver provides.
Embodiment
The applicant be an example with Herb of Resupinate Woodbetony attractive in appearance (another name: too Radix Ginseng), and the method for extraction aucubin is referring to accompanying drawing 1,2, and concrete step is:
1. extract
Get Herb of Resupinate Woodbetony meal raw material attractive in appearance, extract twice with the aether backflow of 8 times of amounts of raw material, each 1h is with weeding of grease solubility impurity, filtrate recycling ethanol.
Herb of Resupinate Woodbetony meal residue attractive in appearance extracts twice with the methyl alcohol cold soaking of 10 times of amounts of raw material respectively, and the time is 24h, filters, merging filtrate, reclaim under reduced pressure methyl alcohol add the acetone of 2 times of amounts of raw material while hot to an amount of in filtrate, as seen white flocks is separated out, and waits to precipitate and separates out complete after-filtration.
With these precipitation ethanol-methyl alcohol (10: 1) recrystallization three times, promptly obtain white needle.
2. differentiate
With the methanol solution of the methanol solution of the white needle of gained and aucubin reference substance respectively point sample on silica gel g thin-layer plate.With chloroform-methanol (7: 2) is developping agent, with Ehrilich reagent (paradimethy laminobenzaldehyde 1g, be dissolved in the mixing solutions of 36% hydrochloric acid of 25ml and 75ml methyl alcohol and get) be developer, 85 ℃ of colour developings down, all be blue spot, and both Rf values are consistent, are aucubin so extract the white needle of gained.
Claims (1)
1. a method of extracting aucubin from pedicularis plants is characterized in that, carries out according to the following steps:
1) get Herb of Resupinate Woodbetony meal raw material, extract twice with the aether backflow of 8 times of amounts of raw material, each 1h is with the weeding of grease solubility impurity;
2) residue extracts twice with the methyl alcohol cold soaking of 10 times of amounts of raw material respectively, and the time is 24h, filters, merging filtrate, reclaim under reduced pressure methyl alcohol add the acetone of 2 times of amounts of raw material while hot to an amount of in filtrate, as seen white flocks is separated out, and waits to precipitate and separates out complete after-filtration;
3) usefulness ethanol of the throw out after the filtration and recrystallizing methanol are three times, wherein the ethanol of mixing solutions: methyl alcohol=10: 1, can obtain white needle, and identify through thin-layer chromatography, be aucubin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100173772A CN101225095B (en) | 2008-01-22 | 2008-01-22 | Method for extracting aucubin from pedicularis plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100173772A CN101225095B (en) | 2008-01-22 | 2008-01-22 | Method for extracting aucubin from pedicularis plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101225095A true CN101225095A (en) | 2008-07-23 |
| CN101225095B CN101225095B (en) | 2011-06-22 |
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ID=39857328
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100173772A Expired - Fee Related CN101225095B (en) | 2008-01-22 | 2008-01-22 | Method for extracting aucubin from pedicularis plants |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982471A (en) * | 2010-09-30 | 2011-03-02 | 天津理工大学 | Technological process for extracting aucubin from fruits of eucommia ulmoides oliver |
| CN102068450A (en) * | 2010-11-17 | 2011-05-25 | 山东省科学院生物研究所 | Application of aucubin to preparation of anti-thrombosis medicament |
| CN102786505A (en) * | 2012-09-05 | 2012-11-21 | 中国科学院西北高原生物研究所 | Preparation method for separating three flavone monomeric compounds from flos pedicularis |
| CN103349705A (en) * | 2013-07-23 | 2013-10-16 | 王琪 | Application of active site of pedicularis verticillata in preparation of medicine, cosmetic and health care products |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100218052B1 (en) * | 1992-07-15 | 1999-09-01 | 이병언 | Pharmaceutical preparation inhibiting duplication of hbv |
-
2008
- 2008-01-22 CN CN2008100173772A patent/CN101225095B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101982471A (en) * | 2010-09-30 | 2011-03-02 | 天津理工大学 | Technological process for extracting aucubin from fruits of eucommia ulmoides oliver |
| CN101982471B (en) * | 2010-09-30 | 2013-02-13 | 天津理工大学 | Technological process for extracting aucubin from fruits of eucommia ulmoides oliver |
| CN102068450A (en) * | 2010-11-17 | 2011-05-25 | 山东省科学院生物研究所 | Application of aucubin to preparation of anti-thrombosis medicament |
| CN102786505A (en) * | 2012-09-05 | 2012-11-21 | 中国科学院西北高原生物研究所 | Preparation method for separating three flavone monomeric compounds from flos pedicularis |
| CN102786505B (en) * | 2012-09-05 | 2014-12-17 | 中国科学院西北高原生物研究所 | Preparation method for separating three flavone monomeric compounds from flos pedicularis |
| CN103349705A (en) * | 2013-07-23 | 2013-10-16 | 王琪 | Application of active site of pedicularis verticillata in preparation of medicine, cosmetic and health care products |
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| Publication number | Publication date |
|---|---|
| CN101225095B (en) | 2011-06-22 |
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Granted publication date: 20110622 Termination date: 20140122 |