CN101199539B - Application of locust glycolipid in preparing anti-skin ringworm medicament - Google Patents
Application of locust glycolipid in preparing anti-skin ringworm medicament Download PDFInfo
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- CN101199539B CN101199539B CN2007101147963A CN200710114796A CN101199539B CN 101199539 B CN101199539 B CN 101199539B CN 2007101147963 A CN2007101147963 A CN 2007101147963A CN 200710114796 A CN200710114796 A CN 200710114796A CN 101199539 B CN101199539 B CN 101199539B
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Abstract
The invention discloses an application of sophorolipids in preparing drugs for resisting dermatophyte, and an application of a combination where the sophorolipids is used as an effective component in preparing drugs for resisting dermatophyte. With low price, little dosage and remarkable antibacterial effect, the invention has no side effect on the human, and in certain aspects can be used as a drug for replacing conventional antifungal drugs which produce heat resistance easily and drugs for treating skin tinea disease such as corticosteroid with severe side effects. Thus, the invention has great application prospect.
Description
Technical field
The present invention relates to utilize the application of the biosurfactant sophorolipid that Wickerhamiella domercqiae var. sophorolipid (Wickerhamiella domercqiae var.sophorolipid) fermentation produces, relate in particular to the application of sophorolipid in the anti-dermatophytosis medicine of preparation.
Background technology
Sophorolipid is a kind of important biosurfactant, starts from twentieth century five, the sixties for its research, mainly is to obtain by microbial fermentation.Compare with the surfactant of chemosynthesis, sophorolipid has many advantages such as surface property is good, environmental friendliness.
In recent years, research about the sophorolipid bacteriostasis is reported to some extent, the applicant had also once carried out preliminary study to the effect of sophorolipid inhibition antibacterial, and had applied for Chinese invention patent " application of sophorolipid in the preparation antibiotic medicine, the patent No. 200710013200.0 " in January, 2007.Yet through document and patent retrieval, the research that utilizes the sophorolipid biosurfactant of microorganisms to be used to prepare anti-dermatophytosis (particularly fungus) medicine yet there are no report.
The infection that present dermatophytosis clinically causes is increasing, and the medicine kind that is used for the treatment of tinea is single, a lot of patients are under the torment of misery, selected for use for oral administration or the injection corticosteroid hormone, not only the result controls bad, side effect such as face is swollen, hypertension, dizziness also can occur, cause the state of an illness to increase the weight of.Therefore, research and development substitutes and easily to produce chemical sproof conventional antifungal drug and to have the medicine of treatment dermatophytosis such as corticosteroid hormone of serious side effects significant.
Summary of the invention
At deficiency described in the prior art, the problem to be solved in the present invention provides the application of a kind of sophorolipid in the anti-dermatophytosis medicine of preparation.
The application of sophorolipid of the present invention in the anti-dermatophytosis medicine of preparation.Also comprise with the sophorolipid being the application of compositions in the anti-dermatophytosis medicine of preparation of effective ingredient simultaneously.
Wherein, described tinea is tinea capitis, tinea corporis, tinea cruris, tinea unguium and/or tinea manus and pedis; Described dermatophytosis is a fungus.
Wherein, described fungus mainly is meant trichophyton, Sabouraudites lanosus, trichophyton gypseum and/or pityrosporum furfur.
In the above-mentioned application, the drug level that described sophorolipid effectively suppresses dermatophytosis is 0.1～5.0g/L; The preferred drug level that effectively suppresses dermatophytosis is 0.2～4.0g/L; The drug level of most preferred effective inhibition dermatophytosis is 0.5～3.0g/L.
Utilize sophorolipid adding acceptable accessories of the present invention or excipient can make said dosage form on any pharmaceutics.
With sophorolipid of the present invention is that effective ingredient adds the pharmaceutical composition that antifungal component and acceptable accessories or excipient composition press down dermatophytosis, equally also can make said dosage form on any pharmaceutics.
One of the preferred ointment of the dosage form of anti-dermatophytosis medicine of the present invention, liquid preparation.
Wherein, liquid preparation preferred liquid liniment.
The pharmaceutical preparation of above-mentioned ointment, liquid preparation can be according to the known method preparation of the industry, in preparation process, use pharmaceutically acceptable auxiliaries or excipient, for example adhesive (as pregelatinized Starch, polyvinylpyrrolidone or hydroxypropyl cellulose), filler (for example lactose, sucrose, mannitol etc.), lubricant (for example stearic acid, Polyethylene Glycol, magnesium stearate, Pulvis Talci or silicon dioxide), disintegrating agent (for example starch, sodium carboxymethyl cellulose) or wetting agent (for example sodium lauryl sulphate).
In order to understand essence of the present invention better, pharmacological evaluation and the result with sophorolipid illustrates the application of sophorolipid as the anti-dermatophytosis medicine that suppresses trichophyton, Sabouraudites lanosus, trichophyton gypseum, pityrosporum furfur below.
The preparation of sophorolipid of the present invention:
(1) strain is selected: Wickerhamiella domercqiae var. sophorolipid Wickerhamiella domercqiae var.sophorolipid CGMCC No.1576;
(2) slant culture: bacterial classification inoculation in the basic inorganic salt culture medium, is wherein added mass volume ratio and is 1.5～2.0% agar, under 25～35 ℃ of conditions, static cultivation 24～48 hours;
(3) seed culture: with the bacterial strain of step (2) cultivation, under aseptic condition, encircle in 20～100mL and contain in the basic inorganic salt culture medium of Oleum Brassicae campestris that mass volume ratio is 4～14% glucose and 1～8% with inoculation articulating 1～2, under 25～35 ℃ of conditions, shaken cultivation 24～48 hours makes seed liquor;
(4) amplification culture: the inoculum concentration of volume ratio with 5～10%, seed liquor is inoculated in 100～1000mL to be contained in the basic inorganic salt culture medium of Oleum Brassicae campestris that mass volume ratio is 4～14% glucose and 1～8%, under 25～35 ℃ of conditions, shaken cultivation 96～288 hours;
(5) collect tunning: the fermentation liquid of step (4) gained is left standstill, make its natural subsidence, collect and be deposited on the light yellow thick liquid that shakes the bottle bottom, with 4000～7000 rev/mins that described thick substances is centrifugal afterwards, the collecting precipitation part is sophorolipid crude extract;
(6) distilling under reduced pressure: with step (5) gained sophorolipid crude extract, at 50～70 ℃ of temperature, pressure 0.2～0.8Kg/cm
2, vacuum is to carry out distilling under reduced pressure, evaporative removal moisture wherein under the condition of 0.02～0.08Mpa;
(7) gleanings drying: with the distilling under reduced pressure residue of above-mentioned collection, at 50～100 ℃, under vacuum 0.02～0.08Mpa condition, vacuum drying 12～24 hours makes the solid sophorolipid.
The thalline preparation of dermatophytosis:
Trichophyton, Sabouraudites lanosus, trichophyton gypseum and the pityrosporum furfur of inclined-plane preservation are respectively got an inoculating loop to be transferred to respectively in the husky Bao Shi culture medium of liquid, cultivated 10～20 hours down at 25～37 ℃, make the bacteria suspension of trichophyton, Sabouraudites lanosus, trichophyton gypseum and pityrosporum furfur respectively.
The dermatophytosis bacteriostatic test:
The sophorolipid that the inventive method makes is made mother solution with the dimethyl sulfoxine dissolving, standby.
Get the sophorolipid mother solution and be added to respectively in the husky Bao Shi solid plate culture medium, fall dull and stereotyped and make each dull and stereotyped final concentration be the gradient concentration of 0.1～5.0g/L.Getting trichophyton, Sabouraudites lanosus, trichophyton gypseum and each 0.1mL of pityrosporum furfur suspension is applied in the solid plate culture medium of above-mentioned concentration in gradient, cultivated 12～120 hours down at 25～35 ℃, observe the growing state of the dermatophytosis on the flat board, calculate average clump count.
The prescription of the husky Bao Shi culture medium of aforesaid liquid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, pH 6.5.
The prescription of the husky Bao Shi culture medium of above-mentioned solid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, agar powder 20g/L, pH 6.5.
The bacteriostatic test result shows:
The sophorolipid (1g/L) that has added than low dosage is dull and stereotyped in the long period (120h), and a small amount of colony growth is only arranged, and when adding sophorolipid concentration when reaching 2g/L, the growth of trichophyton is suppressed (see figure 1) fully.
For Sabouraudites lanosus, trichophyton gypseum and pityrosporum furfur, on sophorolipid (0.5g/L) flat board that has added than low dosage, its growth promptly can be suppressed fully (see Fig. 2, Fig. 3, Fig. 4).
In the application of sophorolipid of the present invention in the anti-dermatophytosis medicine of preparation, sophorolipid utilizes the Wickerhamiella domercqiae var. sophorolipid fermenting and producing, have the advantages that process is easy, cost is low, purity is high, therefore the preparation of preparation is cheap, and consumption is few, fungistatic effect is remarkable, and is without any side effects to human body.It is developed as pharmaceutical dosage forms such as clinical external wash liquid, ointment, can be in some aspects easily produce stable on heating conventional antifungal drug as an alternative and the medicine of the treatment dermatophytosis such as corticosteroid hormone of serious side effects is arranged, have great application prospect.
Description of drawings
Fig. 1 sophorolipid flat board is to the inhibition of trichophyton growth
Show that sophorolipid concentration under 2g/L, can suppress the growth of trichophyton fully.
A does not add the contrast of sophorolipid; B has added the contrast of dimethyl sulfoxine; It is the sophorolipid of formula 1.0% that C has added concentration; It is 2.0% sophorolipid that D has added concentration.
Fig. 2 sophorolipid flat board is to the inhibition of Sabouraudites lanosus growth
Show that sophorolipid concentration under 1.0g/L, can suppress the growth of Sabouraudites lanosus fully.
A does not add the contrast of sophorolipid; B has added the contrast of dimethyl sulfoxine; It is 0.5% sophorolipid that C has added concentration; It is 1.0% sophorolipid that D has added concentration.
Fig. 3 sophorolipid flat board is to the inhibition of trichophyton gypseum growth
Show that sophorolipid concentration under 1.0g/L, can suppress the growth of trichophyton gypseum fully.
A does not add the contrast of sophorolipid; B has added the contrast of dimethyl sulfoxine; It is 0.5% sophorolipid that C has added concentration; It is 1.0% sophorolipid that D has added concentration.
Fig. 4 sophorolipid is to the inhibition of pityrosporum furfur growth
Show that sophorolipid concentration under 1.0g/L, can suppress the growth of pityrosporum furfur fully.
A does not add the contrast of sophorolipid; B has added the contrast of dimethyl sulfoxine; It is 0.5% sophorolipid that C has added concentration; It is 1.0% sophorolipid that D has added concentration.
The specific embodiment
Below in conjunction with embodiment content of the present invention is further described.
Embodiment 1: the preparation of sophorolipid
(1) strain is selected: Wickerhamiella domercqiae var. sophorolipid (Wickerhamiella domercqiae var.sophorolipid) CGMCC No.1576;
(2) slant culture: bacterial classification inoculation in the basic inorganic salt culture medium, is wherein added mass volume ratio and is 1.6% agar, under 25 ℃ of conditions, static cultivation 24 hours;
(3) seed culture: the bacterial strain that step (2) is cultivated, under aseptic condition, to encircle in 20mL and contain in the culture medium of Oleum Brassicae campestris that mass volume ratio is 4% glucose and 1% with inoculating articulating 1～2, under 25 ℃ of conditions, shaken cultivation 24 hours makes seed liquor;
(4) amplification culture: the inoculum concentration of volume ratio with 5%, seed liquor is inoculated in 100mL contains in the culture medium that mass volume ratio is 1% Oleum Brassicae campestris, under 25 ℃ of conditions, shaken cultivation 96 hours;
(5) collect tunning: the fermentation liquid of step (4) gained is left standstill, make its natural subsidence, collect and be deposited on the light yellow thick liquid that shakes the bottle bottom, with 5000 rev/mins that above-mentioned thick substances is centrifugal afterwards, collect solid-state part, be sophorolipid crude extract.
(6) distilling under reduced pressure: with step (5) gained sophorolipid crude extract, at 60 ℃ of temperature, pressure 0.4kg/cm
2, vacuum is to carry out distilling under reduced pressure, evaporative removal moisture wherein under the condition of 0.04Mpa;
(7) gleanings drying: with the distilling under reduced pressure residue of above-mentioned collection, at 70 ℃, under the vacuum 0.04Mpa condition, vacuum drying 16 hours; Make the solid sophorolipid.
Embodiment 2: the preparation of sophorolipid
(1) strain is selected: Wickerhamiella domercqiae var. sophorolipid (Wickerhamiella domercqiae var.sophorolipid) CGMCC No.1576;
(2) slant culture: bacterial classification inoculation in the basic inorganic salt culture medium, is wherein added mass volume ratio and is 1.6% agar, under 30 ℃ of conditions, static cultivation 36 hours;
(3) seed culture: the bacterial strain that step (2) is cultivated, under aseptic condition, to encircle in 50mL and contain in the culture medium of Oleum Brassicae campestris that mass volume ratio is 8% glucose and 3% with inoculating articulating 1～2, under 30 ℃ of conditions, shaken cultivation 36 hours makes seed liquor;
(4) amplification culture: the inoculum concentration of volume ratio with 7%, seed liquor is inoculated in 500mL contains in the culture medium that mass volume ratio is 3% Oleum Brassicae campestris, under 30 ℃ of conditions, shaken cultivation 200 hours;
(5) collect tunning: the fermentation liquid of step (4) gained is left standstill, make its natural subsidence, collect and be deposited on the light yellow thick liquid that shakes the bottle bottom, with 6000 rev/mins that above-mentioned thick substances is centrifugal afterwards, collect solid-state part, be sophorolipid crude extract.
(6) distilling under reduced pressure: with step (5) gained sophorolipid crude extract, at 70 ℃ of temperature, pressure 0.8kg/cm
2, vacuum is to carry out distilling under reduced pressure, evaporative removal moisture wherein under the condition of 0.08Mpa;
(7) gleanings drying: with the distilling under reduced pressure residue of above-mentioned collection, at 100 ℃, under the vacuum 0.08Mpa condition, vacuum drying 24 hours; Make the solid sophorolipid.
Embodiment 3: sophorolipid is as the application of the anti-dermatophytosis medicine that suppresses trichophyton
The thalline preparation of trichophyton: the trichophyton of inclined-plane preservation is got an inoculating loop be transferred in the husky Bao Shi culture medium of liquid, cultivated 20 hours down, make the trichophyton suspension at 37 ℃.
The sophorolipid that embodiment 1 makes is made mother solution with the dimethyl sulfoxine dissolving, standby.
Get the sophorolipid mother solution and be added to respectively in the husky Bao Shi solid medium, fall dull and stereotyped and make each ware final concentration be the gradient concentration of 0.1～5.0g/L.Get trichophyton suspension 0.1mL and be applied in the solid plate culture medium of above-mentioned concentration in gradient, cultivated 120 hours down, observe the growing state of the dermatophytosis on the flat board, calculate average clump count at 35 ℃.
The prescription of the husky Bao Shi culture medium of aforesaid liquid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, pH 6.5.
The prescription of the husky Bao Shi culture medium of above-mentioned solid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, agar powder 20g/L, pH 6.5.
The bacteriostatic test result shows:
The sophorolipid (1.0g/L) that has added than low dosage is dull and stereotyped in the long period (120h), and a small amount of colony growth is only arranged, and when adding sophorolipid concentration when reaching 2.0g/L, the growth of trichophyton is suppressed (see figure 1) fully.
Embodiment 4: sophorolipid is as the application of the anti-dermatophytosis medicine that suppresses Sabouraudites lanosus
The thalline preparation of Sabouraudites lanosus: the Sabouraudites lanosus of inclined-plane preservation is got an inoculating loop be transferred in the husky Bao Shi culture medium of liquid, cultivated 20 hours down, make the Sabouraudites lanosus suspension at 30 ℃.
The sophorolipid that embodiment 1 makes is made mother solution with the dimethyl sulfoxine dissolving, standby.
Get the sophorolipid mother solution and be added to respectively in the husky Bao Shi solid medium, fall dull and stereotyped and make each ware final concentration be the gradient concentration of 0.1～5.0g/L.Get Sabouraudites lanosus suspension 0.1mL and be applied in the solid plate culture medium of above-mentioned concentration in gradient, cultivated 120 hours down, observe the growing state of the Sabouraudites lanosus on the flat board, calculate average clump count at 30 ℃.
The prescription of the husky Bao Shi culture medium of aforesaid liquid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, pH 6.5.
The prescription of the husky Bao Shi culture medium of above-mentioned solid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, agar powder 20g/L, pH 6.5.
The bacteriostatic test result shows:
Sabouraudites lanosus is on sophorolipid (0.5g/L) flat board that has added than low dosage, and its growth promptly can be suppressed (see figure 2) fully.
Embodiment 5: sophorolipid is as the application of the anti-dermatophytosis medicine that suppresses trichophyton gypseum
The thalline preparation of trichophyton gypseum: the trichophyton gypseum of inclined-plane preservation is got an inoculating loop be transferred in the husky Bao Shi culture medium of liquid, cultivated 10～20 hours down, make the trichophyton gypseum suspension at 25～37 ℃.
The sophorolipid that embodiment 1 makes is made mother solution with the dimethyl sulfoxine dissolving, standby.
Get the sophorolipid mother solution and be added to respectively in the husky Bao Shi solid medium, fall dull and stereotyped and make each ware final concentration be the gradient concentration of 0.1～5.0g/L.Get trichophyton gypseum suspension 0.1mL and be applied in the solid plate culture medium of above-mentioned concentration in gradient, cultivated 120 hours down, observe the growing state of the trichophyton gypseum on the flat board, calculate average clump count at 32 ℃.
The prescription of the husky Bao Shi culture medium of aforesaid liquid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, pH 6.5.
The prescription of the husky Bao Shi culture medium of above-mentioned solid (being used for fungal culture) is: peptone 10g/L, glucose 40g/L, agar powder 20g/L, pH6.5.
The bacteriostatic test result shows:
Trichophyton gypseum is on sophorolipid (0.5g/L) flat board that has added than low dosage, and its growth promptly can be suppressed (see figure 3) fully.
Embodiment 6: sophorolipid is as the application of the anti-dermatophytosis medicine that suppresses pityrosporum furfur
The thalline preparation of pityrosporum furfur: the pityrosporum furfur of inclined-plane preservation is got an inoculating loop be transferred in the husky Bao Shi culture medium of liquid, cultivated 20 hours down, make the pityrosporum furfur suspension at 27 ℃.
The sophorolipid that embodiment 2 makes is made mother solution with the dimethyl sulfoxine dissolving, standby.
Get the sophorolipid mother solution and be added to respectively in the husky Bao Shi solid medium, fall dull and stereotyped and make each ware final concentration be the gradient concentration of 0.1～5.0g/L.Get pityrosporum furfur suspension 0.1mL and be applied in the solid plate culture medium of above-mentioned concentration in gradient, cultivated 120 hours down, observe the growing state of the pityrosporum furfur on the flat board, calculate average clump count at 27 ℃.
The prescription of the husky Bao Shi culture medium of aforesaid liquid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, pH 6.5.
The prescription of the husky Bao Shi culture medium of above-mentioned solid (being used for fungal culture) is: peptone 10g/L, and glucose 40g/L, agar powder 20g/L, pH 6.5.
The bacteriostatic test result shows:
Pityrosporum furfur is on sophorolipid (0.5g/L) flat board that has added than low dosage, and its growth promptly can be suppressed (see figure 4) fully.
Embodiment 7: sophorolipid is as the preparation of the anti-dermatophytosis drug ointment agent of treatment trichophyton infection
Getting sophorolipid 45g that embodiment 1 produces adds liquid Paraffin 125g and is heated to 80 ℃, other gets glycerol 50g, ethyl hydroxybenzoate 1g, adding distil water also is heated to 80 ℃ to 830g, then oil phase is added aqueous phase, the limit edged is stirred to cold, the plastic flexible pipe of packing into, every pipe charge weight is 25g, promptly gets the sophorolipid ointment.
Embodiment 8: sophorolipid is as the preparation of the anti-dermatophytosis drug ointment agent of treatment Sabouraudites lanosus infection
Getting sophorolipid 40g that embodiment 2 produces adds liquid Paraffin 125g and is heated to 80 ℃, other gets glycerol 50g, ethyl hydroxybenzoate 1g, adding distil water also is heated to 80 ℃ to 835g, then oil phase is added aqueous phase, the limit edged is stirred to cold, the plastic flexible pipe of packing into, every pipe charge weight is 25g, promptly gets the sophorolipid ointment.
Embodiment 9: sophorolipid is as the preparation of the anti-dermatophytosis drug ointment agent of treatment trichophyton gypseum
Getting sophorolipid 40g that embodiment 1 produces adds liquid Paraffin 125g and is heated to 80 ℃, other gets glycerol 50g, ethyl hydroxybenzoate 1g, adding distil water also is heated to 80 ℃ to 835g, then oil phase is added aqueous phase, the limit edged is stirred to cold, the plastic flexible pipe of packing into, every pipe charge weight is 25g, promptly gets the sophorolipid ointment.
Embodiment 10: sophorolipid is as the preparation of the anti-dermatophytosis drug ointment agent of treatment pityrosporum furfur
Getting sophorolipid 40g that embodiment 1 produces adds liquid Paraffin 125g and is heated to 80 ℃, other gets glycerol 50g, ethyl hydroxybenzoate 1g, adding distil water also is heated to 80 ℃ to 835g, then oil phase is added aqueous phase, the limit edged is stirred to cold, the plastic flexible pipe of packing into, every pipe charge weight is 25g, promptly gets the sophorolipid ointment.
Embodiment 11: the anti-dermatophytosis pharmaceutical liquid lotion preparation that sophorolipid infects as the treatment trichophyton
Get the sophorolipid 45g that embodiment 1 produces, add dimethyl sulfoxine, make the sophorolipid dissolving, in the 100mL plastic bottle of packing into, promptly to 1000g.
Embodiment 12: the anti-dermatophytosis pharmaceutical liquid lotion preparation that sophorolipid infects as the treatment Sabouraudites lanosus
Get the sophorolipid 40g that embodiment 2 produces, add dimethyl sulfoxine, make the sophorolipid dissolving, in the 100mL plastic bottle of packing into, promptly to 1000g.
Embodiment 13: the anti-dermatophytosis pharmaceutical liquid lotion preparation that sophorolipid infects as the treatment trichophyton gypseum
Get the sophorolipid 40g that embodiment 1 produces, add dimethyl sulfoxine, make the sophorolipid dissolving, in the 100mL plastic bottle of packing into, promptly to 1000g.
Embodiment 14: the anti-dermatophytosis pharmaceutical liquid lotion preparation that sophorolipid infects as the treatment pityrosporum furfur
Get the sophorolipid 40g that embodiment 2 produces, add dimethyl sulfoxine, make the sophorolipid dissolving, in the 100mL plastic bottle of packing into, promptly to 1000g.
Embodiment 15: be the preparation of the compositions of effective ingredient as the agent of anti-dermatophytosis drug ointment with the sophorolipid
Get sophorolipid 4g and clotrimazole 10g that embodiment 1 produces, add liquid Paraffin 125g and be heated to 80 ℃.Other gets glycerol 50g, ethyl hydroxybenzoate 1g, adding distil water also is heated to 80 ℃ to 835g, then oil phase is added aqueous phase, the limit edged is stirred to paste, the plastic flexible pipe of packing into, every pipe charge weight is 10g, promptly gets the sophorolipid ointment, wherein contain sophorolipid 0.1 gram (0.4%), clotrimazole 0.25g (1%).
Embodiment 16: the application of sophorolipid preparation in zoopery
The anti-dermatophytosis pharmaceutical liquid of the sophorolipid lotion of getting the conduct treatment trichophyton infection of producing among the embodiment 11 is used for zoopery.
Get 60 of mices, cut little wound, the inoculation trichophyton every Mus right fore oxter.From wound sampling carrying out microexamination, find that having 45 mices to infect has trichophyton behind the 72h, wound has fur to come off after the week, forms the tinea piece.Have the mice of trichophyton to be divided into 9 groups infection, 5 groups as processed group, divides every day with above-mentioned sophorolipid liquid preparation and embrocates for three times, other 4 groups as negative control group, carry out embrocating of same number with normal saline.Successive administration 10 days.Observe processed group and matched group wound healing situation, and, cultivate with the husky Bao Shi solid plate of cultivating fungus respectively from processed group and the sampling of matched group wound.
Experimental result shows that processed group is aseptic dropping out now in 24h, between the 96h, has only fragmentary bacterium colony to occur at 24h in the flat board of 5 groups of processed group, and average clump count is 1.Matched group has a large amount of bacterium colonies to occur in 24h, can't count.
Claims (6)
1. the application of sophorolipid in the anti-trichophyton of preparation, Sabouraudites lanosus, trichophyton gypseum and/or pityrosporum furfur medicine.
2. with the sophorolipid application of compositions in the anti-trichophyton of preparation, Sabouraudites lanosus, trichophyton gypseum and/or pityrosporum furfur medicine of effective ingredient.
3. application as claimed in claim 1 or 2 is characterized in that, the drug level that described sophorolipid effectively suppresses described bacterium is 0.1～5g/L.
4. application as claimed in claim 3 is characterized in that, the drug level that described sophorolipid effectively suppresses described bacterium is 0.2～4g/L.
5. application as claimed in claim 4 is characterized in that, the drug level that described sophorolipid effectively suppresses described bacterium is 0.5～3g/L.
6. application as claimed in claim 1 or 2 is characterized in that the dosage form of described anti-dermatophytosis medicine is an ointment.
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|---|---|---|---|---|
| EP0209783A1 (en) * | 1985-07-24 | 1987-01-28 | Wella Aktiengesellschaft | Use of sophoroselipid-lactone for the treatment of dandruffs and body odour |
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| 陈静等.酵母胞外槐糖脂产生条件优化及其抑菌作用.山东大学学报(理学版)40 3.2005,40(3),第116-120页. * |
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