CN101194924A - Use of lespedeza virgata in preparing medicament for activating blood circulation and removing stasis - Google Patents
Use of lespedeza virgata in preparing medicament for activating blood circulation and removing stasis Download PDFInfo
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Abstract
The invention provides the usage of a traditional Chinese medicine lespedeza virgata in the process of preparing a medicament with the function of improving blood circulation and relieving stasis. The medicament can dramatically lower 'blood stasis' and the state of 'concentration, viscosity, accumulation and condensate' of blood, which can change blood rheology, and has the functions of anti-thrombotic, inhibiting platelet to aggregate, improving local microcirculation of body, and dilating blood capillary. The invention has an obvious protective effect to acute myocardial ischemia which is caused by ligation of coronary arteries.
Description
Technical field
The present invention relates to a kind of new purposes of Chinese crude drug, specifically is the new purposes of Lespedeza virgata (Thunb. ex Murray) DC..
Background technology
Kind surplus the pulse family lespedeza plant about 60 is distributed in East Asia to Australian northeast and North America, and China produces 26 kinds, except that Xinjiang, is distributed widely in each provinces and regions, the whole nation.Modern pharmacology studies show that Caulis Seu Folium Lespedezae Bicoloris has higher medical value: the flavone in the Caulis Seu Folium Lespedezae Bicoloris has treatment renal insufficiency and antiphlogistic effect; The ethanol extraction of lespedeza cuneata root is to the various animals and through the selective excitation of the isolated uterine of diethylstilbestrol sensitization of being pregnant, and the isolated uterines of various unpregnancy animals is not had obvious effect; The tannin of extracting from Da Ye Caulis Seu Folium Lespedezae Bicoloris root bark has significant antiearly pregnancy effect; Has the analgesic effect from the total flavones of Caulis Seu Folium Lespedezae Bicoloris and the extraction of spire Caulis Seu Folium Lespedezae Bicoloris aerial parts; Lespedeza virgata (Thunb. ex Murray) DC. has the effect of moistening lung and clearing heat, relieving stranguria by diuresis, invigorating the spleen to clear away damp pathogen, is usually used in treating nephritis and malaria among the people.
Summary of the invention
The purpose of this invention is to provide the new purposes of Lespedeza virgata (Thunb. ex Murray) DC. in pharmacy.
New purposes of the present invention is the purposes of Lespedeza virgata (Thunb. ex Murray) DC. [Lespedeza virgata (Thurb) DC] in the medicine of preparation blood circulation promoting and blood stasis dispelling.
Modern medical theory thinks, blood stasis mainly is that " dense, sticking, coagulate, poly-" phenomenon has taken place blood, and promptly hemorheology is unusual, and it can cause the blood stasis of blood to stagnate, and blood circulation and energy metabolism disorder are one of major reasons that causes cardiovascular and cerebrovascular disease.The inventor finds, Lespedeza virgata (Thunb. ex Murray) DC. can obviously reduce " blood stasis " rat blood " dense, sticking, poly-, with fixed attention " state, and the change hemorheology has the effect of blood circulation promoting and blood stasis dispelling, can be used to prepare the medicine of blood circulation promoting and blood stasis dispelling.The inventor also further finds; Lespedeza virgata (Thunb. ex Murray) DC. has antithrombotic, anticoagulant, improves the body local microcirculation, expands blood capillary; can protect the acute myocardial ischemia that causes because of the ligation arteria coronaria significantly; can be used to prepare anticoagulation or antithrombotic medicine, especially treat the medicine of cardiovascular disease.
Drug main of the present invention will be made up of medicinal powder, water extract or the ethanol extraction of Lespedeza virgata (Thunb. ex Murray) DC. and medically acceptable adjuvant.Described medicinal powder, water extract or the ethanol extraction content in medicine is 5～200g crude drug/g.
Medicine of the present invention can also contain other medicines that helps blood circulation promoting and blood stasis dispelling, as the active component by the preparation of medical materials such as the Radix Astragali, Lignum Dalbergiae Odoriferae and Ramulus Cinnamomi.
The preparation method of Lespedeza virgata (Thunb. ex Murray) DC. water extract of the present invention can be water extraction or decoction and alcohol sedimentation technique.
Described water extraction concrete steps are: get the Lespedeza virgata (Thunb. ex Murray) DC. medicinal material coarse powder, add 5～8 times of water gagings or add water logging and cross powder 2～5cm, soak 0.5～3h, be heated to and boil, keep little and boiled about 0.5～1.5 hour, the leaching medicinal liquid is standby; The slag of getting it filled decocts 1～2 time with the method water, and each 1～2h filters, and merging filtrate staticly settles and removes impurity, concentrates drying.
Described decoction and alcohol sedimentation technique concrete steps are: get the Lespedeza virgata (Thunb. ex Murray) DC. medicinal material coarse powder, add 5～8 times of water gagings or add water logging and cross powder 2～5cm, soak 0.5～3h, be heated to and boil, keep little and boiled about 0.5～1.5 hour, the leaching medicinal liquid is standby; The slag of getting it filled decocts with the method water, and totally 1～2 time, each 1～2h, filter, merging filtrate, concentrated filtrate to 1～2g crude drug/ml, the concentration of adding and concentrated back liquid volume equivalent is 75%～95% ethanol (also available gradient incremental method precipitate with ethanol) then, left standstill 12～24 hours, inclining supernatant, filters, and reclaims ethanol, concentrate drying.
The preparation method of ethanol extraction of the present invention can be ethanol refluxing process or ethanol extraction.
Described ethanol refluxing process concrete steps are: get the Lespedeza virgata (Thunb. ex Murray) DC. medical material, be ground into coarse powder, use 50%～95% ethanol extraction, 1～3h respectively, extract 2～3 times, merge extractive liquid, left standstill 24～48 hours, filtered, and reclaimed ethanol, concentrated drying;
Described ethanol extraction concrete steps are: get the Lespedeza virgata (Thunb. ex Murray) DC. medical material, in 60%～90% alcoholic solution, stir, soak at room temperature 24h, lixiviate 1～2 time, (but also gradient lixiviate, as distinguishing room temperature lixiviate 24h with 60%, 90% ethanol successively), merge extractive liquid, filters then, reclaim ethanol, concentrate drying.
The extracting solution of gained can also be refining with organic solvent extractionprocess before dry after concentrating in the above method, and concrete steps are: water extract after will concentrating or alcohol extract organic solvent extraction, get organic facies, and remove organic solvent; Described organic solvent can be one or more in n-butyl alcohol, ethyl acetate, the chloroform.
To further specify effect, the especially anticoagulation of the blood circulation promoting and blood stasis dispelling of Lespedeza virgata (Thunb. ex Murray) DC., antithrombotic effect and the effect in the treatment cardiovascular disease below by zoopery.
1, Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of " blood stasis " hemorheology of rat
(1) Lespedeza virgata (Thunb. ex Murray) DC. administration pre-treatment: get Lespedeza virgata (Thunb. ex Murray) DC. medical material 100g, use 50%, 70%, 95% alcohol reflux 1.5h respectively, filtering and concentrating is to 40ml, lucifuge, airtight, cryopreservation is stand-by, face with preceding redilution to become desired concn.
(2) get the healthy SD rat, male and female half and half, body weight (230 ± 15) g is divided into 5 groups at random, 9 every group:
A: normal control group;
B: model group;
C: Lespedeza virgata (Thunb. ex Murray) DC. high dose group (4.5gkg
-1)
D: middle dosage group (2.25gkg
-1)
E: ligustrazine group (20mgkg
-1).
Except that normal control group and model group, respectively organize gastric infusion 1 time every day, successive administration 7d.Except that the normal control group, each organizes rat sc 0.8mgkg behind last administration 1h
-1Epinephrine inj is injected 1 time behind the 6h at interval again, and rat being put into frozen water therebetween stimulates 5min, fasting.Anaesthetize with 20% urethane intravenous injection morning next day, carotid artery blood sampling 4mL, add in the anticoagulant heparin pipe, measure the different shearforces of whole blood viscosity (η 6), plasma viscosity (η p), packed cell volume (HCT), erythrocyte electrophoretic time (EPT), erythrocyte aggregation index (AI), Kazon yield stress rheology indexs such as (FC).The results are shown in Table 1.
Table 1 Caulis Seu Folium Lespedezae Bicoloris to the influence of " blood stasis " hemorheology of rat (
N=9)
| Group | η 6η(mpa·s-1) | p mpa·s -1 | HCT ％ | EPT s | AI | FC pa | ||
| 3 | 30 | 200 | ||||||
| A | 13.61± 1.03 | 6.38±0.79 | 4.96±0.58 | 1.55±0.19 | 45.61±3.0 3 | 17.57±2.6 1 | 7.89±0.57 | 11.13±1.16 |
| B | 15.31± 1.57 1) | 7.12±0.53 1) | 5.71± 0.49 1) | 1.82± 0.10 2) | 51.26± 4.68 1) | 20.03± 1.79 1) | 8.38±0.62 | 13.67± 0.97 1) |
| C | 12.12± 0.62 4) | 5.98±0.41 4) | 4.62± 0.31 4) | 1.61± 0.06 4) | 42.39± 2.99 4) | 16.14± 0.86 4) | 5.89± 0.46 4) | 10.27± 0.41 4) |
| D | 13.09± 0.78 3) | 6.20±0.69 3) | 4.74± 0.47 4) | 1.72± 0.13 3) | 45.13± 3.17 3) | 17.22± 1.79 1) | 7.58± 0.67 3) | 11.01± 0.63 4) |
| E | 13.16± 0.66 3) | 6.38±0.52 3) | 5.13± 0.51 3) | 1.74± 0.09 3) | 45.77± 3.12 3) | 18.56± 1.53 | 7.52± 0.60 3) | 12.11± 0.82 3) |
Compare with matched group,
1)P＜0.05,
2)Compare with model group P＜0.01,
3)P＜0.05,
4)P＜0.01.
Experimental result shows, Caulis Seu Folium Lespedezae Bicoloris can obviously reduce whole blood viscosity, blood viscosity, packed cell volume, Kazon yield stress, erythrocyte electrophoretic time (P＜0.01 of the basic, normal, high shear rate of " blood stasis " rat, P＜0.05) and erythrocyte aggregation index (P＜0.05) prompting Caulis Seu Folium Lespedezae Bicoloris can reduce " blood stasis " rat plasma viscosity and concentration, suppress its erythrocytic gathering and solidify, thereby improve the body blood rheological characteristic, reduce blood " dense, sticking, poly-, with fixed attention " state, have certain function of promoting blood circulation to disperse blood clots.
2, Lespedeza virgata (Thunb. ex Murray) DC. influences microcirculation of mouse auricle
(1) Lespedeza virgata (Thunb. ex Murray) DC. administration pre-treatment: get Lespedeza virgata (Thunb. ex Murray) DC. medical material 100g, use 50%, 70%, 95% alcohol reflux 1.5h respectively, filtering and concentrating is to 40ml,, lucifuge, airtight, cryopreservation is stand-by, face with preceding redilution to become desired concn.
(2) healthy KM mice is selected in experiment for use, and male and female half and half are divided into 5 groups according to the experiment needs at random with animal:
A: model control group (waiting the capacity distilled water);
B: Radix Salviae Miltiorrhizae tablet group (1.00gkg
-1);
C: Lespedeza virgata (Thunb. ex Murray) DC. group (4.5g crude drug kg
-1);
D: Lespedeza virgata (Thunb. ex Murray) DC. group (2.25g crude drug kg
-1);
E: Lespedeza virgata (Thunb. ex Murray) DC. group (1.13g crude drug kg
-1).
Each mice is used pentobarbital sodium intraperitoneal injection of anesthesia (35mgkg
-1), cut off the outside fine, soft fur of auricle gently with eye scissors, then do the duodenal intubation operation.The mice ventricumbent position is fixed on the observation platform, drip a little liquid paraffin in auricle portion, aim at auricle with the cold light source of PVC-3 type microcirculation brain comprehensive analyzer, then with the microscope alignment auricle, bring into focus, observed blood capillary is carried out caliber, flow velocity, liquid mensuration with computer.Again by tail vein injection 10% high molecular dextran 4mgkg
-1, inject by duodenum when waiting blood stasis symptom stablize behind the 10min and be subjected to the reagent thing, after the observation administration 30,60 and the microcirculation situation of 9min, with the significant 60min of onset as the observed and recorded time.
Liquid standards of grading are divided into the linear flow state for " 0 ", and " 1 " is divided into the dotted line stream mode, and " 2 " are divided into grain (wadding) shape stream, and " 3 " are divided into the stagnant shape of the stasis of blood; No erythrocyte flows through in the blood capillary.
Table 2 Lespedeza virgata (Thunb. ex Murray) DC. to dextran induced mice Auricle Microcirculation influence (
N=20)
| Group | Caliber (μ m) | Flow velocity (μ m/s) * 10 | Fluidised form | ||||||
| Before the administration | After the modeling 30 ' | After the administration 60 ' | Before the administration | After the modeling 30 ' | After the administration 60 ' | Before the administration | After the modeling 30 ' | After the administration 60 ' | |
| A | 15.89±1.7 | 13.80±1.4 | 13.88±1.6 | 1.16±0.16 | 0.67±0.06 | 0.65±0.08 | 0 | 2.17±0.53 | 2.12±0.41 |
| B | 15.83±1.6 | 13.70±1.5 | 15.91±1.3 ** | 1.18±0.15 | 0.68±0.08 | 0.93±0.11 ** | 0 | 2.23±0.61 | 0.68±0.43 ** |
| C | 15.79±1.5 | 13.70±1.4 | 15.76±1.3 ** | 1.16±0.13 | 0.67±0.07 | 0.99±0.12 ** | 0 | 2.29±0.52 | 0.57±0.46 ** |
| D | 15.87±1.8 | 13.90±1.5 | 15.77±1.4 | 1.15±0.17 | 0.68±0.05 | 0.89±0.08 ** | 0 | 2.25±0.53 | 0.76±0.69 ** |
| E | 15.76±1.5 | 13.60±1.3 | 14.73±1.1 | 1.17±0.18 | 0.66±0.04 | 0.78±0.92 | 0 | 2.16±0.47 | 1.21±0.51 * |
Compare with model group
*P＜0.05,
*P＜0.01
The result is as shown in table 2, linearly flows before the modeling of Mice Auricle blood capillary, and flow velocity does not have cohesion soon, behind the sub-dextran that awards high marks, blood flow the stasis of blood occurs and stagnates, and has many red blood clots to occur, and blood vessel wall fogs, caliber dwindles, and flow velocity significantly slows down, part blood vessel even appearance swing or stagnation.After giving Caulis Seu Folium Lespedezae Bicoloris extract to irritate stomach (every day 2.25 or 4.5g/kg), agglomerate reduces, and flow velocity is accelerated, tube wall is clear, and caliber enlarges, and has improved by the blood stasis due to the high molecular dextran, with evident difference is relatively arranged before the administration, act on suitable with Radix Salviae Miltiorrhizae Tabellae.
3, Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of mice normal pressure anoxia enduring
(1) Lespedeza virgata (Thunb. ex Murray) DC. administration pre-treatment: get Lespedeza virgata (Thunb. ex Murray) DC. medical material 100g, use 50%, 70%, 95% alcohol reflux 1.5h respectively, filtering and concentrating is to 40ml, lucifuge, airtight, cryopreservation is stand-by, face with preceding redilution to become desired concn.
(2) healthy KM mice is selected in experiment for use, and male and female half and half are divided into 5 groups at random: model control group, Radix Salviae Miltiorrhizae Tabellae group, the large, medium and small dosage group of Caulis Seu Folium Lespedezae Bicoloris.Each group is administered once in advance by table 8 dosage, test the back 30min that was administered once again the same day, each organizes the equal subcutaneous injection isoprenaline of mice 20mgkg-1, gets a white mice for every group at every turn and puts into the 125m grournd glass respectively (bottle is put into 25g NaOH and 25g CaCl in advance
2, to absorb moisture and CO
2, be encased inside filter paper above it, draw urine), use the vaseline seal cover tight after putting into immediately, the mice time-to-live in the opening entry bottle.As seen each group carries out statistical analysis: and Lespedeza virgata (Thunb. ex Murray) DC. (4.5,2.25gkg
-1Group) mean survival time of animal has tangible prolongation effect (the results are shown in Table 8) than model control group.The result is as shown in table 3, and Lespedeza virgata (Thunb. ex Murray) DC. can prolong the time-to-live of mice normal pressure anoxia enduring significantly, has the good Mice Auricle microcirculation that improves.
Table 3 Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of anoxia enduring time-to-live of mice normal pressure
| Group | Dosage/gkg-1 | Number of animals/n | Mean survival time/min |
| Model control group Radix Salviae Miltiorrhizae Tabellae group Caulis Seu Folium Lespedezae Bicoloris group (greatly) Caulis Seu Folium Lespedezae Bicoloris group (in) Caulis Seu Folium Lespedezae Bicoloris group (little) | - 1.00 4.5 2.25 1.13 | 20 20 20 20 20 | 12.48±2.96 15.49±3.06 * 16.98±3-47 ** 15.53±1.89 * 13.40±2.57 |
Compare with model group
*P＜0.05,
*P＜0.01
4, the anticoagulation of Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. and anti thrombotic action
(1) Lespedeza virgata (Thunb. ex Murray) DC. administration pre-treatment: get Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. medical material 100g, add the 800ml water boil, the slag of getting it filled decocts 2 times with method, and filtering and concentrating is to 100ml, add 100ml concentration then and be 95% ethanol, standing over night, inclining supernatant, filters, remove tannin, reclaim the aqueous solution that makes 1.5g/ml behind the ethanol, lucifuge, airtight, cryopreservation is stand-by, face with preceding redilution to become desired concn.
(2) anticoagulation of Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. and anti thrombotic action experiment
1, experimental technique
Frozen water-epinephrine type rat suppository forms the model moulding: get 60 of SD rats, and male and female half and half, body weight 180 ~ 220g divides 6 groups by the body weight stratified random, 10 every group: 1. normal group (the 1ml/100g body weight is irritated the stomach distilled water); 2. model group (1ml/100g body weight, irritate stomach distilled water); 3. Aspirin Enteric-coated Tablets group (0.05gkg
-1Body weight); 4. ~ 6. the large, medium and small dosage group of Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. (5.5g crude drug kg
-1, 2.75g crude drug/kg, 1.38g crude drug kg
-1).Gastric infusion, every day 1 time, 8d continuously.All the other respectively organized equal subcutaneous injection adrenalin hydrochloride 0.5mgkg except that normal group in the 7th day
-1Body weight, totally 2 times, interval 8h.The isometric normal saline of normal group subcutaneous injection.Water is can't help in fasting, spends the night.Behind the 8th day last administration 1h, except that normal group, all the other respectively organize equal subcutaneous injection adrenalin hydrochloride 0.5mg/kg body weight, the isometric normal saline of normal group subcutaneous injection, immerse behind the 20min and make swimming 5min in the frozen water, after pulling about 20 minutes out (little the doing of Mus whole body this moment), with 25% urethane (0.5ml/100g body weight) anesthetized animal.
(1) cutting off rat tail (apart from terminal 0.5 centimeters) extrudes 2 and bleeds and drip on sheet glass (diameter 4-5mm) respectively, autoblood drops on the slide and picks up counting, pass through drop of blood every 30s with the 1ml syringe needle, and choose blood once, as see that needle point provokes fiber protein yarn, stop timing, writing time.Provoke another blood of not choosing again and compare, and with the last report as a result of of bleeding.
(2) abdominal aortic blood: 1. get not anticoagulant of 1ml,, immediately blood is injected in the rotating ring according to the Chandler in vitro method, sealing is put on the thrombosis instrument rapidly, with the rotating speed rotation 15min of 17 ± 1rpm, incline and thrombosis, remove surplus blood with the filter paper suction, survey length L, claim weight in wet base w with slide gauge, calculate thrombosis index (thrombosis index Q=L/2+W1/2), thrombosis is put 80 ℃ of baking oven 1h, weigh, be the thrombosis dry weight.All the other blood put into that (preparation of anticoagulant test tube: the 10.0% sodium citrate 0.5ml that respectively gets ultrasonic dissolution is put in 70 disposable plastic tubes of label with the test tube of 10.0% sodium citrate anticoagulant (ratio of anticoagulant and whole blood is 1: 9), shake up to make and adhere on the test tube wall dry for standby in 60-80 ℃ of baking oven).2. getting 1ml10.0% sodium citrate anticoagulation adds in the long-neck ball vial, place platelet adhesion instrument rotating disk, the rotating speed rotation 15min of 5rpm, inject 1% ammonium oxalate dilution 0.4ml respectively at quantitatively getting anticoagulation 20 μ l before and after the rotation, measure the platelet count in the blood of rotation front and back, calculate platelet adhesion rate, platelet adhesion rate=(changeing thromboblast number-commentaries on classics back platelet count)/change thromboblast to count X 100%.3. get blood 3ml, wherein 1ml blood injects the test tube (ratio of anticoagulant and whole blood is 1: 9) that is placed with 3.8% sodium citrate anticoagulant.2ml injects and contains indometacin-EDTA-Na
20.2ml in vitro, 4 ℃, 3500r/min, centrifugal 15 minutes, separated plasma.Carry out the mensuration of prothrombin time PT, thrombin time TT, KPTT KPTT by the reagent kit description.
2, experimental result
2.1 Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of the relevant thrombosis of frozen water one epinephrine type rat suppository model: table 4 as seen, the thrombosis length of model group rat, wet weight of thrombus, thrombosis dry weight and thrombosis index are all apparently higher than normal group (P＜0.01); Thrombosis length, wet weight of thrombus, thrombosis dry weight and the thrombosis index of heavy dose of group of Lespedeza virgata (Thunb. ex Murray) DC. and Aspirin Enteric-coated Tablets group all are starkly lower than model group (P＜0.01); In the Lespedeza virgata (Thunb. ex Murray) DC., wet weight of thrombus, thrombosis dry weight and the thrombosis index of small dose group all be starkly lower than or be lower than model group (P＜0.01 or P＜0.05); The wet weight of thrombus of the large, medium and small dosage group of Lespedeza virgata (Thunb. ex Murray) DC., thrombosis dry weight and thrombosis index are compared with the Aspirin Enteric-coated Tablets group does not have significant difference (P＞0.05).Show that Lespedeza virgata (Thunb. ex Murray) DC. has anti thrombotic action to frozen water one epinephrine type rat, and effect is suitable with aspirin.
Table 4 Lespedeza virgata (Thunb. ex Murray) DC. to the influence of frozen water one epinephrine type rat suppository model (
N=10)
| Group | Dosage | Length (mm) | Weight in wet base (mg) | Dry weight (mg) | Index |
| Normal group model group aspirin Lespedeza virgata (Thunb. ex Murray) DC. Lespedeza virgata (Thunb. ex Murray) DC. Lespedeza virgata (Thunb. ex Murray) DC. | -- -- 0.05 5.50 2.75 1.38 | 30.6±21.7** 78.3±23.6 25.8±17.6** 32.7±20.1** 38.7±17.8** 62.5±31.9 | 1037.9±723.3** 3269.1±885.0 799.6±296.8 1016.3±659.7** 1398.2±987.5** 1411.2±833.9** | 132.1±118.2** 633.7±313.8 99.2±57.1** 162.6±121.0** 361.2±189.5* 458.7±263.9 | 48.6±23.1** 97.3±21.2 40.5±12.0** 46.7±28.3** 63.5±18.2* 69.2±25.7* |
Annotate: compare * * P＜0.01, * P＜0.05 with model group
2.2 Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of rat platelet adhesion rate, whole blood coagulation time: the result is as shown in table 5, and the platelet adhesion rate of model group is compared with other group, does not have significant difference (P＞0.05); The model group whole blood coagulation time is significantly less than normal group (P＜0.05); The whole blood coagulation time of dosage group is compared notable difference (P＜0.05) in Aspirin Enteric-coated Tablets group and the Lespedeza virgata (Thunb. ex Murray) DC. with model group: the whole blood coagulation time of the heavy dose of group of Lespedeza virgata (Thunb. ex Murray) DC. has been compared notable difference (P＜0.01) with model group; The whole blood coagulation time of the large, medium and small dosage group of Lespedeza virgata (Thunb. ex Murray) DC. is compared with the Aspirin Enteric-coated Tablets group does not have significant difference (P＞0.05).Show that Lespedeza virgata (Thunb. ex Murray) DC. has tangible blood coagulation resisting function, and effect is suitable with aspirin.
Table 5 Lespedeza virgata (Thunb. ex Murray) DC. to the influence of rat platelet adhesion rate, whole blood coagulation time (
N=10)
| Group | Dosage | Platelet adhesion rate/% | Whole blood coagulation time (s) |
| Normal group model group aspirin group Lespedeza virgata (Thunb. ex Murray) DC. group Lespedeza virgata (Thunb. ex Murray) DC. group Lespedeza virgata (Thunb. ex Murray) DC. group | -- -- 0.05 5.5 2.75 1.38 | 15.9±9.7 18.3±11.3 11.6±8.4 12.1±10.2 15.2±7.8 16.3±11.4 | 407.8±165.2 * 255.7±110.6 508.1±238.5 ** 511.5±131.2 ** 436.2±164.9 * 369.1±172.1 |
Annotate: compare * * P＜0.01, * P＜0.05 with model group
2.3 Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of PT, TT and KPTT: table 6 as seen, the PT of model group, TT, KPTT are significantly shorter than normal group (P＜0.01 or P＜0.05); PT, TT, the KPTT of each group of Aspirin Enteric-coated Tablets group and Lespedeza virgata (Thunb. ex Murray) DC. have compared notable difference (P＜0.01 or P＜0.05) with model group; PT, the TT of the large, medium and small dosage group of Lespedeza virgata (Thunb. ex Murray) DC., KPTT compare with the Aspirin Enteric-coated Tablets group does not have significant difference (P＞0.05).Show: Lespedeza virgata (Thunb. ex Murray) DC. can prolong thrombin time TT and KPTT KPTT, and PT, and effect is suitable with aspirin.
Table 6 Lespedeza virgata (Thunb. ex Murray) DC. to the influence of PT, TT, KPTT (
N=10)
| Group | Dosage | PT(s) | TT(s) | KPTT(s) |
| Normal group model group aspirin Lespedeza virgata (Thunb. ex Murray) DC. group Lespedeza virgata (Thunb. ex Murray) DC. group Lespedeza virgata (Thunb. ex Murray) DC. group | -- -- 0.05 5.5 2.75 1.38 | 52.3±20.9 88.3±69.7 56.1±33.2 54.9±23.8 61.4±37.6 69.2±31.5 | 113.7±33.5 **49.2±32.5 101.4±39.9 **117.3±58.2 **107.4±51.8 **72.5±33.2 | 59.9±21.0 * 39.1±10.3 68.4±31.2 * 99.5±61.2 ** 78.3±43.6 ** 70.1±53.2 * |
Annotate: compare * * P＜0.01, * P＜0.05 with model group
5, Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of rat platelet aggregation
(1) preparation method of Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC.: get Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. medical material 100g, use 50%, 70%, 95% alcohol reflux 1.5h respectively, filtering and concentrating is to 40ml, lucifuge, airtight, cryopreservation is stand-by, face with preceding redilution to become desired concn.
(2) method and result: get healthy male SD rat, body weight (215 ± 15) g is divided into 4 groups at random, 9～10 every group: drinking water matched group, aspirin group 0.01g/kg), Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. 4.5g crude drug/kg group and 2.25g crude drug/kg group.Each organizes continuous gastric infusion 7 days, every day 1 time, and behind last administration 1h, with 3% pentobarbital 30mg/kg difference anesthetized rat, through carotid artery blood sampling 4mL, 3.8% sodium citrate anticoagulant, the ratio of anticoagulant and blood is 1: 9.With the centrifugal 5min of 800r/min, preparation separates platelet rich plasma (PRP) with anticoagulation, and part PRP with the centrifugal 10min of 4000r/min, is prepared platelet poor plasma (PPP).Transfer PRP to make platelet count with PPP 6～7 * 10
8/ mL adds 200 μ L PRP in assembling pipe, behind 37 ℃ of incubation 5min, add ADP (4 μ molL subsequently
-1) induce gathering, press Born
[5]Platelet maximum agglutination rate (MPAG) in the turbidimetry for Determination 5min reaches time (Tm) of maximum agglutination rate, and 1,3, platelet aggregation rate (PAG during 5min
1, PAG
3, PAG
5).The results are shown in Table 7, Caulis Seu Folium Lespedezae Bicoloris has antiplatelet aggregative activity.
Table 7 Caulis Seu Folium Lespedezae Bicoloris is to the influence of rat platelet aggregation
| Group | Dosage g/kg | Number of animals only | MPAG ％ | Tm min | PAG 1 ％ | PAG 3 ％ | PAG 5％ |
| Matched group | - | 10 | 56.8±9.1 | 4.33±0.78 | 42.8±12.5 | 56.6±13.0 | 53.9±16.3 |
| Aspirin | 0.01 | 9 | 47.3± 6.7 ** | 2.95±1.13 ** | 39.8±6.1 | 45.2±9.4 ** | 40.9±13.3 ** |
| The Caulis Seu Folium Lespedezae Bicoloris group | 4.5 | 10 | 46.5± 6.4 ** | 2.98±0.92 ** | 36.2±8.5 | 42.1±8.5 ** | 39.6±14.0 ** |
| The Caulis Seu Folium Lespedezae Bicoloris group | 2.25 | 10 | 49.9±4.7 * | 3.34±1.10 * | 39.6±6.6 | 43.3±11.2 ** | 42.8±11.7 ** |
Annotate: compare * * P＜0.01, * P＜0.05 with matched group
6, Lespedeza virgata (Thunb. ex Murray) DC. is to the influence of rat suppository formation
(1) preparation method of Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC.: get Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. medical material 100g, use 95%, 75%, 60% alcohol reflux 1.5h respectively, filtering and concentrating is to 40ml, lucifuge, airtight, cryopreservation is stand-by, face with preceding redilution to become desired concn.
(2) get healthy male SD rat, body weight (240 ± 20) g is divided into 4 groups at random, 10 every group (the grouping situation is identical with last experiment).Behind last administration 1h, with 3% pentobarbital 30mgkg
-1Behind the anesthetized rat, separate a bilateral common carotid artery respectively, carotid artery is provoked gently with two electrodes by electrostimulation, 1.5mA galvanic stimulation 7min, (Occlusion Time OT), promptly begins to stimulate the time that descends suddenly to temperature from electric current to measure the occluding thrombus formation time.With the length of OT as judging that medicine is to thrombotic curative effect index.OT prolongs %=(administration group OT-matched group OT)/matched group OT * 100%.The results are shown in Table 6.
Table 8 Caulis Seu Folium Lespedezae Bicoloris to the influence of rat carotid artery thrombosis (
N=10)
| Group | Dosage (gKG-1) | Thrombus formation time (min) | Rate elongation (%) |
| Matched group Caulis Seu Folium Lespedezae Bicoloris group Caulis Seu Folium Lespedezae Bicoloris group aspirin group | 4.5 2.25 0.01 | 10.79±0.98 16.17±1.82 ** 14.98±1.77 ** 15.23±2.01 ** | 49.86 38.83 41.15 |
Annotate: compare * * P＜0.01, * P＜0.05 with matched group
The result is as shown in table 8, and Caulis Seu Folium Lespedezae Bicoloris has the obvious suppression effect to the thrombosis due to the arteries endothelial injury, and the prompting Caulis Seu Folium Lespedezae Bicoloris has anti thrombotic action, and this effect may be relevant with the protection vascular endothelial injury.
7, Lespedeza virgata (Thunb. ex Murray) DC. produces the protective effect of acute myocardial ischemia to the ligation rat coronary artery
(1) preparation method of Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC.: get Chinese medicine Lespedeza virgata (Thunb. ex Murray) DC. medical material 100g, use 50%, 70%, 95% alcohol reflux 1.5h respectively, filtering and concentrating is to 40ml, lucifuge, airtight, cryopreservation is stand-by, face with preceding redilution to become desired concn.
(2) the healthy SD rat is selected in experiment for use, and male and female half and half are divided into model control group, Radix Salviae Miltiorrhizae group, Caulis Seu Folium Lespedezae Bicoloris (large, medium and small dosage) group, 10 every group at random.Behind various dose and group gastric infusion 1h, urethane 0.65gkg-1 intraperitoneal injection of anesthesia.Back of the body position is fixing, with more than half rubber bulb (just in time entangling the rat incidence), connect artificial respirator after, the chest unhairing, behind the routine disinfection,,, open the thoracic cavity at the pure property of the 4th intercostal separation flesh layer along rat left mid-clavicular line longitudinal incision skin 2cm, cut off pericardium, gently push the right side thoracic wall, extrude heart.Coronary vein place ligation left coronary artery between arterial cone and left atrium.Immediately heart is sent back to the thoracic cavity, sew up thoracic wall rapidly, stop artificial respirator respectively at immediate postoperative, 2,5,8,12 and 24h measure and respectively to organize rat standard limbs II lead electrocardiogram, observe the typical myocardial ischemia electrocardiogram persistent period of animal, respectively organize rat heart muscle ischemia recovery time and number of animals.Every group in postoperative equal every day of gastric infusion 2 times.The results are shown in Table 9.Radix Salviae Miltiorrhizae Tabellae and Lespedeza virgata (Thunb. ex Murray) DC. cause that to the ligation arteria coronaria rat heart muscle ischemia all has protective effect.From postoperative 8h just, to postoperative 24h, most of animal recovers normally substantially, and Lespedeza virgata (Thunb. ex Murray) DC. effect heavy dose of and middle dosage group is suitable substantially in the Radix Salviae Miltiorrhizae Tabellae group.
Table 9 Caulis Seu Folium Lespedezae Bicoloris produces the protective effect of acute myocardial ischemia to the ligation rat coronary artery
| Group | Dosage/gkg -1 | The Mus number (n) of different time myocardial ischemia behind the ligation arteria coronaria | ||||||
| 0h | 2h | 5h | 8h | 12h | 24h | Dead | ||
| Model control group Radix Salviae Miltiorrhizae group Caulis Seu Folium Lespedezae Bicoloris group (greatly) Caulis Seu Folium Lespedezae Bicoloris group (in) Caulis Seu Folium Lespedezae Bicoloris group (little) | - 1.00 4.5 2.25 1.13 | 10 10 10 10 10 | 10 10 10 10 10 | 10 8 7 7 9 | 10 6 5 6 8 | 9 5 3 3 7 | 8 1 0 1 3 | 2(16h) 0 0 0 1(24h) |
More than the used stasis syndrome model of experiment is that to be mainly " yang deficiency cold coagulation " according to the traditional Chinese medical science about the cause of disease, the pathogenesis of syndrome of blood stasis designed.Epinephrine is α, beta receptor analeptic, have the effect of shrinking peripheral blood vessel and increasing cardiac contractile force, therefore, giving on the basis of rat ice-water bath, add and use the epinephrine subcutaneous injection, peripheral blood vessel is shunk strongly, and cardiac afterload increases, and the myocardial oxygen consumption metabolism increases, cardiac function descends gradually, thereby make the blood operation obstacle, blood viscosity increases, and forms the pathological change of blood stasis.Experimental result shows, Lespedeza virgata (Thunb. ex Murray) DC. can obviously reduce " blood stasis " rat blood " dense, sticking, poly-, coagulate " state, changes hemorheology, and visible Lespedeza virgata (Thunb. ex Murray) DC. has certain function of promoting blood circulation to disperse blood clots.Simultaneously; experimental result also further shows; Lespedeza virgata (Thunb. ex Murray) DC. has antithrombotic, anticoagulant, improves the body local microcirculation, expands blood capillary, can protect the acute myocardial ischemia that causes because of the ligation arteria coronaria significantly, and its pharmaceutical preparation can be used for the treatment of cardiovascular and cerebrovascular disease.
8, Caulis Seu Folium Lespedezae Bicoloris extract different separation component anticoagulant active experimental study behind organic solvent extraction
(1) system of Lespedeza virgata (Thunb. ex Murray) DC. different component separates
With Caulis Seu Folium Lespedezae Bicoloris raw material stirring and leaching 24h in 80% alcoholic solution, lixiviate twice obtains ethanol extraction and residue respectively.Ethanol extraction is soluble in water, adopt petroleum ether, chloroform, ethyl acetate, n-butyl alcohol to extract successively according to the ascending order of polarity then, each organic layer solution of gained is removed organic solvent, carried out vacuum drying again by rotary evaporation, obtain petroleum ether extract, chloroform extract, acetic acid ethyl ester extract and n-butyl alcohol extract, its separation process as shown in Figure 1.With each extract anhydrous alcohol solution of gained, water dilutes, and is made into the solution of 0.1g crude drug/ml.
(2) research of the external anticoagulation of Lespedeza virgata (Thunb. ex Murray) DC. system separation component
5 of the mensuration SD rats of prothrombin time (PT), abdominal vein is got blood, place the plastic tube of the 0.109mol/l sodium citrate anti-freezing liquid (1 part of anti-freezing liquid+9 part whole blood) that contains 1/10 volume respectively, put upside down mixing gently, centrifugal 15 minutes of 3000rpm, collect upper strata liquid (blood plasma, yellow), stand-by.In vitro add blood plasma 0.1ml to be measured, add Chinese medicine extract 0.1ml, put 37 ℃ of water-bath 1-2 minutes, add calcic histothrombin solution 0.2ml fast, fully shake up immediately and start stopwatch, put 37 ℃ of water-baths, shake test tube gently, about 8-10 took out during second, side is moved test tube, starts manual time-keeping, observes and the recording start setting time, this survey of every increment 3 times is averaged.The results are shown in Table 10.
5 of the mensuration SD rats of activated partial thromboplastin time (KPTT), abdominal vein is got blood, place the plastic tube of the 0.109mol/l sodium citrate anti-freezing liquid (1 part of anti-freezing liquid+9 part whole blood) that contains 1/10 volume respectively, put upside down mixing gently, centrifugal 15 minutes of 3000rpm, collect upper strata liquid (blood plasma, yellow), stand-by.10 bottles of lyophilizing partial thromboplastins and 0.025M calcium chloride are formed in every test kit, before using in every bottle adding distil water 1.5ml shake up and dissolve, put 37 ℃, pre-temperature is standby, in vitro adds blood plasma 0.1ml to be measured, adds Chinese medicine extract 0.1ml, the kaolin partial prothrombinase suspension 0.1ml that adds mixing again, mixing is put 37 ℃ and was hatched at least 3 minutes, after hatching, add 0.1ml calcium chloride solution (0.025M), mixing starts manual time-keeping, the record PCT.The results are shown in Table 10.
5 of the mensuration SD rats of thrombin time (TT), abdominal vein is got blood, place the plastic tube of the 0.109mol/l sodium citrate anti-freezing liquid (1 part of anti-freezing liquid+9 part whole blood) that contains 1/10 volume respectively, put upside down mixing gently, centrifugal 15 minutes of 3000rpm, collect upper strata liquid (blood plasma, yellow), stand-by.Every bottle of TT reagent adds the 2.5ml buffer, leaves standstill 5-10 second, the jog dissolving, by the time 5u/ml thrombin dissolving in vitro adds 37 ℃ of pre-warm blood plasma 0.2ml to be measured, adds Chinese medicine extract 0.1ml, add TT reagent 0.2ml, start manual time-keeping, the record setting time.The results are shown in Table 10.
The mensuration of table 10 prothrombin time (PT)
| Different component | PT | APTT | TT |
| Normal saline petroleum ether extract chloroform extract acetic acid ethyl ester extract n-butyl alcohol extract | 5.00±3.7 9.84±5.6 18.19±8.3 * 38.10±17.1 * 46.84±16.3 * | 31.65±7.3 52.40±13.7 99.33±37.2 * 118.56±38.5 * 107.65±27.6 * | 10.37±2.3 19.13±15.8 46.12±23.8 * 107.86±47.3 * 110.18±67.2 * |
Annotate: compare * P＜0.01 with the normal saline group
The result shows after Caulis Seu Folium Lespedezae Bicoloris extract is made with extra care through chloroform, ethyl acetate or n-butanol extraction all have good blood coagulation resisting function, especially the extract of n-butyl alcohol.
Description of drawings
Fig. 1 is the technological process of adopting chloroform, ethyl acetate and n-butanol extraction Lespedeza virgata (Thunb. ex Murray) DC. ethanol extraction successively.
The specific embodiment
Example 1
Prescription: Lespedeza virgata (Thunb. ex Murray) DC. 1960g, Radix Astragali 1000g, Lignum Dalbergiae Odoriferae 300g, Ramulus Cinnamomi 500g
Method: above 4 flavors, adding water 25L respectively decocted 1 hour, leach medicinal liquid, the slag of getting it filled again boiled 1 hour with the 15L decocting, merged all medicinal liquids, centrifugal removing slag, get extracting solution 32L, with the microporous filter membrane of 0.45um filter clear liquor, under 294kPa (3kg/cm2) pressure, carry out ultrafiltration again, remove molecular weight greater than 50000 impurity.Be evaporated to 10L in 40 ℃, spray drying gets dry thing 420g, adds lactose 180g in dry thing; magnesium stearate 3g presses large stretch of (the heavy 2g of sheet, diameter is 2cm) behind the mixing; this pre-tabletting is broken in oscillating granulator, and granulate gets sieve (20 ~ 50 order) granule 560g 2 ~ No. 3.Use the aluminum-plastic composite membrane splitting, every bag of 2g.
Instructions of taking: warm boiled water, one time 1 bag, 3 times on the one.
Example 2
Get Lespedeza virgata (Thunb. ex Murray) DC. medical material 1900g, add water 13L and decocted 1 hour, leach medicinal liquid, the slag of getting it filled again boiled 1 hour with the 8L decocting, filter, merge filtrate twice, filtrate is concentrated into 1000ml, add 1000ml concentration then and be 95% ethanol, standing over night, inclining supernatant, filter, reclaim ethanol and concentrate, add an amount of mixing of starch, cross sieve No. 7, in 60 ~ 70 ℃ of oven dry, encapsulated, make 1000 altogether, every 0.2g gets final product.
Instructions of taking: oral, one time 4, three times on the one.
Example 3
Get Lespedeza virgata (Thunb. ex Murray) DC. 1900g, add water 13L and decocted 1 hour, leach medicinal liquid, the slag of getting it filled again boiled 1 hour with the 8L decocting, filtered, and merged filtrate twice, the concentration that concentrates 5 times of amounts of back adding is 85% ethanol, fully stirs, and leaves standstill 24h, inclining supernatant, filters, and reclaims ethanol and is condensed into the thick paste shape, be dried to dry extract, add right amount of auxiliary materials, make granule, tabletting, every contains dry extract 0.12g.
Instructions of taking: oral, one time 5,3 times on the one.
Example 4
Get Lespedeza virgata (Thunb. ex Murray) DC., be ground into fine powder, the mixing that sieves is used water pill, drying, and per 100 balls nearly weigh 1g, promptly.
Instructions of taking: oral, a 6g, 3～4 times on the one.
Example 5
Formulation preparation: get Lespedeza virgata (Thunb. ex Murray) DC. medical material 1000g, be ground into coarse powder, use 75% alcohol reflux, added 4 times of amounts of suitable medical material alcohol reflux for the first time 3 hours, added 3 times of amounts of suitable medical material alcohol reflux for the second time 1 hour.Merge ethanol extract, left standstill 48 hours, discard precipitation, supernatant decompression recycling ethanol, reuse 90% ethanol are doubly measured, half times of amount refluxes 2 times, collects backflow, and decompression recycling ethanol adds water and stirs evenly, and leaves standstill.Get supernatant and filter, filtrate concentrates; Other gets sucrose 400g and makes syrup, merges with above-mentioned medicinal liquid, adjusts total amount to 1000ml, stirs evenly, and filters, and is canned, every 10ml, and sterilization, promptly.
Instructions of taking: oral, a 10ml, 2 times on the one.
Example 6
Get Lespedeza virgata (Thunb. ex Murray) DC. medical material 1000g, be ground into coarse powder, use 50%, 70%, 95% alcohol reflux successively, each 1h.Merge ethanol extract, left standstill 48 hours, discard precipitation, add water and stir evenly, leave standstill.Get supernatant and filter, filtrate concentrates; Other gets sucrose 400g and makes syrup, merges with above-mentioned medicinal liquid, adjusts total amount to 1000ml, stirs evenly, and filters, and is canned, every 10ml, and sterilization, promptly.
Instructions of taking: oral, a 10ml, 2 times on the one.
Example 7
It is moistening with 70% ethanol to get Lespedeza virgata (Thunb. ex Murray) DC. medicinal material coarse powder 100g, in the dress seepage tube, adds 70% soak with ethanol 24h, percolation is collected percolate 600ml, decompression recycling ethanol, filter, be concentrated into 300ml, respectively with 1/3 volumes of acetic acid ethyl ester extraction 3 times, combining extraction liquid reclaims ethyl acetate, evaporate to dryness, add 75% dissolve with ethanol, decompression recycling ethanol filters to there not being the alcohol flavor, concentrated solution adds 6～7 times of amount ethanol again, and cold preservation 24 hours filters, reclaim ethanol to there not being the alcohol flavor, add the normal saline dilution, add 1% active carbon, heated and boiled, filter, add normal saline to 300ml, adding the natrium carbonicum calcinatum adjust pH is 6.5, G
3Sintered filter funnel filters, potting, and 105 ℃ of 45min of pressure sterilizing, promptly.
Using method: a 20～40ml is added among 5%～10% Glucose Liquid 500ml and instils, once-a-day.
Example 8
It is moistening with 70% ethanol to get Lespedeza virgata (Thunb. ex Murray) DC. medicinal material coarse powder 100g, in the dress seepage tube, adds 70% soak with ethanol 24h, percolation is collected percolate 600ml, decompression recycling ethanol, filter, be concentrated into 300ml, use 1/3 volume chloroform extraction 3 times respectively, combining extraction liquid reclaims chloroform, evaporate to dryness, add 75% dissolve with ethanol, decompression recycling ethanol filters to there not being the alcohol flavor, concentrated solution adds 6～7 times of amount ethanol again, and cold preservation 24 hours filters, reclaim ethanol to there not being the alcohol flavor, add the normal saline dilution, add 1% active carbon, heated and boiled, filter, add normal saline to 300ml, adding the natrium carbonicum calcinatum adjust pH is 6.5, G
3Sintered filter funnel filters, potting, and 105 ℃ of 45min of pressure sterilizing, promptly.
Using method: a 20～40ml is added among 5%～10% Glucose Liquid 500ml and instils, once-a-day.
Example 9
It is moistening with 70% ethanol to get Lespedeza virgata (Thunb. ex Murray) DC. medicinal material coarse powder 100g, in the dress seepage tube, adds 70% soak with ethanol 24h, percolation is collected percolate 600ml, decompression recycling ethanol, filter, be concentrated into 300ml, use 1/3 volume n-butanol extraction 3 times respectively, merge organic layer, reclaim n-butyl alcohol, evaporate to dryness, add 75% dissolve with ethanol, decompression recycling ethanol filters to there not being the alcohol flavor, concentrated solution adds 6～7 times of amount ethanol again, and cold preservation 24 hours filters, reclaim ethanol to there not being the alcohol flavor, add the normal saline dilution, add 1% active carbon, heated and boiled, filter, add normal saline to 300ml, adding the natrium carbonicum calcinatum adjust pH is 6.5, G
3Sintered filter funnel filters, potting, and 105 ℃ of 45min of pressure sterilizing, promptly.
Using method: a 20～40ml is added among 5%～10% Glucose Liquid 500ml and instils, once-a-day.
Example 10
It is moistening with 70% ethanol to get Lespedeza virgata (Thunb. ex Murray) DC. medicinal material coarse powder 100g, in the dress seepage tube, adds 70% soak with ethanol 24h, percolation is collected percolate 600ml, decompression recycling ethanol, filter, be concentrated into 300ml, use chloroform successively, ethyl acetate and n-butanol extraction, get organic layer, merge evaporate to dryness behind the recovery solvent, add 75% dissolve with ethanol, decompression recycling ethanol filters to there not being the alcohol flavor, concentrated solution adds 6～7 times of amount ethanol again, and cold preservation 24 hours filters, reclaim ethanol to there not being the alcohol flavor, add the normal saline dilution, add 1% active carbon, heated and boiled, filter, add normal saline to 300ml, adding the natrium carbonicum calcinatum adjust pH is 6.5, G
3Sintered filter funnel filters, potting, and 105 ℃ of 45min of pressure sterilizing, promptly.
Using method: a 20～40ml is added among 5%～10% Glucose Liquid 500ml and instils, once-a-day.
Claims (6)
- Lespedeza virgata (Thunb. ex Murray) DC. the preparation blood circulation promoting and blood stasis dispelling medicine in purposes.
- 2. purposes according to claim 1 is characterized in that the purposes of Lespedeza virgata (Thunb. ex Murray) DC. in preparation anticoagulation or antithrombotic medicine.
- 3. purposes according to claim 1 is characterized in that the purposes of Lespedeza virgata (Thunb. ex Murray) DC. in the medicine of preparation treatment cardiovascular disease.
- 4. according to claim 1,2 or 3 described purposes, it is characterized in that described medicine comprises medicinal powder, water extract or the ethanol extraction of Lespedeza virgata (Thunb. ex Murray) DC. and medically acceptable adjuvant.
- 5. purposes according to claim 4 is characterized in that medicinal powder, water extract or the ethanol extraction content in medicine of Lespedeza virgata (Thunb. ex Murray) DC. is 5～200g crude drug/g.
- 6. purposes according to claim 4 is characterized in that described medicine also contains the active component that is prepared by the Radix Astragali, Lignum Dalbergiae Odoriferae and Ramulus Cinnamomi.
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| CN101361786B (en) * | 2008-08-25 | 2010-10-27 | 中国农业科学院草原研究所 | Total-flavone extraction and purification and its monomer separation method from Lespedaza bedysaroides |
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| CN101361786B (en) * | 2008-08-25 | 2010-10-27 | 中国农业科学院草原研究所 | Total-flavone extraction and purification and its monomer separation method from Lespedaza bedysaroides |
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