CN101191135A - Method for producing fuel ethanol by kudzu root synchronization saccharification fermentation - Google Patents

Method for producing fuel ethanol by kudzu root synchronization saccharification fermentation Download PDF

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Publication number
CN101191135A
CN101191135A CNA200610114730XA CN200610114730A CN101191135A CN 101191135 A CN101191135 A CN 101191135A CN A200610114730X A CNA200610114730X A CN A200610114730XA CN 200610114730 A CN200610114730 A CN 200610114730A CN 101191135 A CN101191135 A CN 101191135A
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kudzu vine
root
fermentation
ethanol
kudzu roots
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CN101191135B (en
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陈洪章
汪卫东
付小果
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HUNAN JOHNSON PHARM CO Ltd
Institute of Process Engineering of CAS
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HUNAN JOHNSON PHARM CO Ltd
Institute of Process Engineering of CAS
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Priority to CN200610114730A priority Critical patent/CN101191135B/en
Priority to US12/447,895 priority patent/US8333999B2/en
Priority to PCT/CN2007/002268 priority patent/WO2008061418A1/en
Priority to AU2007324208A priority patent/AU2007324208B2/en
Publication of CN101191135A publication Critical patent/CN101191135A/en
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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Abstract

The invention relates to a method for production of fuel ethanol trough synchronous saccharification and fermentation, which comprises the following steps: Kudzu roots are crushed; water mixed materials are added into the Kudzu roots; HC1 is added into the Kudzu roots; PH value of the Kudzu roots is adjusted; temperature of the Kudzu roots is rose to 45 to 50 DEG C; cellulases are added into the Kudzu roots for enzyme treatment; the temperature of the Kudzu roots is rose to 70 to 90 DEG C again, and alpha-amylases are added into the Kudzu roots for cooking; the temperature of the Kudzu roots is cooled to 30 to 35 DEG C, and maltogenic amylases, (NH4)2SO4, KH2PO4 and yeasts after activation are simultaneously added into the Kudzu roots under aseptic condition for anaerobic fermentation by 48 to 84 hours; ethanol generated by fermentation is distilled and collected. The invention establishes an optimum fermentation condition for production of the fuel ethanol trough synchronous saccharification and fermentation of the Kudzu roots; saccharification and fermentation are carried out simultaneously, and no independent saccharification process is needed; glucoses generated by saccharification is utilized by the yeasts at any moment, thereby the invention has the advantages of capability of improving ethanol concentration in beers, reduction of energy consumption of distillation, reduction of treatment of subsequent waste water, reduction of production cost and favorability of comprehensive utilization of the Kudzu roots, and solves the problems of limited grain sources and expensive price when grain raw materials are used for producing ethanol.

Description

The method of producing fuel ethanol by kudzu root synchronization saccharification fermentation
Technical field
Present method belongs to the field that utilizes of root of kudzu vine resource, particularly a kind of method of producing alcohol fuel with the root of kudzu vine as the raw material simultaneous saccharification and fermentation.
Background technology
The alcohol fuel fermentative production of current China is a raw material with food crop such as corn, paddy, wheats mainly, and what Tenth Five-Year Plan Period, China also built certain scale at northern area is the alcohol fuel pilot project of raw material with the corn.But China has a large population and a few land, cultivated land resource is in short supply, grain-production pressure is huge, single is that the alcohol fuel development space of raw material is subjected to great restriction with grain (mainly being corn and war preparedness grain in storage for years rice), be difficult to support for a long time the sustainable development of China's fuel ethanol industrial, this shows that China greatly develops alcohol fuel just must seek abundanter renewable resources---the Biological resources alcoholic acid raw materials for production that act as a fuel.National conditions of comprehensive China and Biological resources situation are considered and are analyzed, a feasible scheme is the area that is not suitable for planting the high yield food crop selectively at some, suit measures to local conditions, by development high yield, high starch, drought-enduring, impoverishment tolerant, for the starch-containing of grain or contain the sugar alcoholic acid raw materials for production that act as a fuel.Attribute as the root of kudzu vine at tens province raised growths on the south the Changjiang river such as China Jiangxi, Hunan, Guizhou just possesses is a kind of raw material of very potential production alcohol fuel.
The root of kudzu vine (Radix Pueraria) is the subterraneous root of Pueraria lobota platymiscium, is perennial pulse family vine, and the beginning is stated from Shennong's Herbal, is listed in middle product,, is classified as " be food be again medicine " register because of rich in starch and isoflavones components by the Ministry of Health.Pueraria lobota platymiscium (Pueraria), kind surplus the whole world has 30, be distributed in states such as China, Japan and South East Asia, China has 9 kinds and 2 mutation approximately, distribution center for the Pueraria lobota platymiscium, all there is the Pueraria lobota distribution of resource all parts of the country, as elegant jessamine (P.Lobata (will) Ohwi), Pachyrhizua angulatus (P.Thomsoni Berth), edible Pueraria lobota (P.edulisPamp), high eyebrow Pueraria lobota (P.Omeiensis Wang), Yunnan Pueraria lobota (P.peduncularis Crah.ex Benth) etc., wherein elegant jessamine and Pachyrhizua angulatus are the most common, the stock number maximum.The rough estimates Pueraria lobota class total area (wild and cultivation) is about 400,000 squares of hectares, and year resource total amount is more than 1,500,000 tons.The root of kudzu vine has now become the cash crop of the many local focus developments of China, thereby obtains the big area artificial culture.
The main composition of the root of kudzu vine is: starch, Mierocrystalline cellulose, protein and Radix Puerariae isoflavone, also contain a spot of fat, pectin, tannin and alkaloid etc. in addition.Bibliographical information, starch content is about 50～60% in the dried root of kudzu vine, content of cellulose 9～15%, crude protein 5～8%, isoflavone content are 3～5%.And the new root of kudzu vine contains moisture 50～60%, and contents of starch reaches 18.5～27.5%.Since the 1950's,, mainly concentrated on the extraction and the medicinal ingredients pharmacological action aspect thereof of effective constituent to the research of the root of kudzu vine.
Summary of the invention
The method that the purpose of this invention is to provide a kind of producing fuel ethanol by kudzu root synchronization saccharification fermentation, this method has been set up root of kudzu vine simultaneous saccharification and fermentation and has been produced the alcoholic acid optimal conditions of fermentation, saccharification is carried out synchronously with fermentation, saccharifying separately, the glucose that saccharification produces is utilized by yeast at any time, can improve alcohol concn in the karusen, reduce the distillation energy consumption, reduce the processing of follow-up waste water, reduce production costs, help the comprehensive utilization of the root of kudzu vine; Solved simultaneously that to produce in the ethanol grain source with grain raw material limited, an expensive difficult problem.
Technical scheme of the present invention is as follows:
The method of producing fuel ethanol by kudzu root synchronization saccharification fermentation provided by the invention, its step is as follows:
1) fragmentation of raw material: the root of kudzu vine is cut into the 200-250mm segment, rubs then into about broken section of 5～10mm;
2) spice, boiling and enzyme are handled:
The broken root of kudzu vine after rubbing is added tap water mix thoroughly, the proportion by weight of the described tap water and the broken root of kudzu vine is 1: 1～1: 6; The HCl adjusting pH that adds 6mol/L is 4.0～4.5, is warming up to 45～50 ℃, and the ratio adding cellulase that adds 0～3.5U in the broken root of kudzu vine of every gram carries out enzyme processing 10～25min; Be warming up to 70～90 ℃ again, the ratio that adds 2.0～10.0U in the broken root of kudzu vine of every gram adds 70～90 ℃ of following boiling 20～50min of α-Dian Fenmei;
3) simultaneous saccharification and fermentation:
Root of kudzu vine material after the boiling is cooled to 30～35 ℃, under aseptic condition, adds saccharifying enzyme, (NH simultaneously 4) 2SO 4, KH 2PO 4Yeast with after the activation carries out anaerobically fermenting 48～84h under 30～35 ℃ of conditions, the ethanol that fermentation produces is collected in distillation;
The add-on of described saccharifying enzyme is: every Ke Gegen adds 20～100U saccharifying enzyme;
Described (NH 4) 2SO 4Add-on be: per 100 gram roots of kudzu vine add 0.05～0.15g (NH 4) 2SO 4
Described KH 2PO 4Add-on be: per 100 gram roots of kudzu vine add 0.05～0.2gKH 2PO 4
Described zymic add-on is; Per 100 gram roots of kudzu vine add 0.10～0.30g yeast;
The present invention has realized that from the angle of simultaneous saccharification and fermentation root of kudzu vine solid state synchronous diastatic fermentation is converted into the alcoholic acid process, has following characteristics and advantage:
1. the present invention adopts and to be suitable for drought-enduring, the hillside that is unfavorable for the food crop growth of impoverishment tolerant, the root of kudzu vine of sandy bare land growth is the production of fuel ethanol raw material, for raw material for fuel ethanol production provides new direction, thereby solve the problem that exists in the grain fermentation: the grain source is limited, and cost an arm and a leg, so grain fermentation costs height, competitive power is poor, and a large amount of in addition grain consumption can cause that per capita grain possession descends, and influences national food safety etc.Help the hill farmer volume increase simultaneously and increase income again, opened up a road of getting wealth.
2. the present invention passes through fermentation condition: the research of the interpolation of water addition ratio, α-Dian Fenmei add-on, cooking time and temperature, saccharifying enzyme add-on, inoculum size, pH value, cellulase add-on, fermentation time and nutritive salt etc. has obtained the zymotechnique route of optimizing.
3. the present invention adds cellulase before fermentation, and the cellulosic component of degraded root of kudzu vine exterior skin helps the gelatinization of pueraria starch, improves starch utilization ratio.
4. the present invention adopts the synchronous saccharification solid state fermentation to produce ethanol, has omitted saccharification workshop section during the fermentation, and energy consumption reduces; Saccharification and fermentation are carried out simultaneously, and the glucose that saccharification is produced is just utilized by yeast once producing, and can keep lower level, helps preventing microbiological contamination.
5. the present invention adopts the synchronous saccharification solid state fermentation of ethanol through, only need to add a spot of water, make that moisture content reduces greatly in the karusen, in improving karusen in the ethanol content, reduced the distillatory energy consumption, and reduced the treating processes of follow-up waste water, the second stage employ for fermentation residue when reducing production costs provides condition, helps the comprehensive utilization of the root of kudzu vine.
Embodiment
Embodiment 1: get root of kudzu vine 30g, be cut into segment, rub broken section then into about 0.5-1.0cm, the root of kudzu vine and tap water are that 3: 1 ratio adds the tap water spice in mass parts, adding 6mol/LHCl adjusting pH again is 4.5, add cellulase (the 1.5U/g root of kudzu vine) when the material temperature is 45 ℃, insulation is carried out enzyme and is handled 20min; Be warmed up to 90 ℃ again and add amylase boiling (the 5.0U/g root of kudzu vine) 40min, be cooled to 30 ℃ afterwards, under aseptic condition, add saccharifying enzyme (the 20U/g root of kudzu vine), 0.25% dry yeast, 0.1% (NH simultaneously 4) 2SO 4, 0.1%KH 2PO 4, under 35 ℃ of conditions, ferment afterwards, get behind the 72h karusen distill ethanol; Measure ethanol content in the karusen; Obtain 0.091g ethanol/g root of kudzu vine, 6.549g ethanol/100g fermented product, starch utilization ratio are 97.8%.
Embodiment 2: get root of kudzu vine 30g, be cut into segment, rub broken section into about 0.5-1.0cm then, the root of kudzu vine and tap water are that 1: 1 ratio adds the tap water spice in mass parts, and adding 6mol/LHCl, to regulate pH be 4.0, and when the material temperature is 45 ℃, 10min is handled in insulation; As material temperature rise to 90 ℃ adding amylase boiling (the 2.0U/g root of kudzu vine) 40min, be cooled to 30 ℃ afterwards then, under aseptic condition, add saccharifying enzyme (the 65U/g root of kudzu vine), 0.25% dry yeast, 0.05% (NH simultaneously 4) 2SO 4, 0.05%KH 2PO 4, under 35 ℃ of conditions, ferment afterwards, get karusen distillation ethanol behind the 48h, measure ethanol content in the karusen.Obtain 0.071g ethanol/g root of kudzu vine, 5.065g ethanol/100g fermented product, starch utilization ratio are 67.2%.
Embodiment 3: get root of kudzu vine 30g, be cut into segment, rub broken section then into about 0.5-1.0cm, the root of kudzu vine and tap water are that 6: 1 ratio adds the tap water spice in mass parts, adding 6mol/LHCl adjusting pH is 4.3, add cellulase (the 1.5U/g root of kudzu vine) when the material temperature is 48 ℃, insulation is carried out enzyme and is handled 20min; As material temperature rise to 70 ℃ adding amylase boiling (the 5.0U/g root of kudzu vine) 40min, be cooled to 32 ℃ afterwards then, under aseptic condition, add saccharifying enzyme (the 20U/g root of kudzu vine), 0.10% dry yeast, 0.1% (NH simultaneously 4) 2SO 4, 0.1%KH 2PO 4, under 35 ℃ of conditions, ferment afterwards, get karusen distillation ethanol behind the 60h, measure ethanol content in the karusen.Obtain 0.076g ethanol/g root of kudzu vine, 6.098g ethanol/100g fermented product, starch utilization ratio are 83.1%.
Embodiment 4: get root of kudzu vine 30g, be cut into segment, rub broken section then into about 0.5-1.0cm, the root of kudzu vine and tap water are that 3: 1 ratio adds the tap water spice in mass parts, adding 6mol/LHCl adjusting pH is 4.5, add cellulase (the 1.5U/g root of kudzu vine) when the material temperature is 50 ℃, insulation is carried out enzyme and is handled 10min; As material temperature rise to 85 ℃ adding amylase boiling (the 6.5U/g root of kudzu vine) 30min, be cooled to 35 ℃ afterwards then, under aseptic condition, add saccharifying enzyme (the 100U/g root of kudzu vine), 0.35% dry yeast, 0.1% (NH simultaneously 4) 2SO 4, 0.1%KH 2PO 4, under 30 ℃ of conditions, ferment afterwards, get karusen distillation ethanol behind the 72h, measure ethanol content in the karusen.Obtain 0.087g ethanol/g root of kudzu vine, 6.032g ethanol/100g fermented product, starch utilization ratio are 90.5%.
Embodiment 5: get root of kudzu vine 30g, be cut into segment, rub broken section then into about 0.5-1.0cm, the root of kudzu vine and tap water are that 3: 1 ratio adds the tap water spice in mass parts, adding 6mol/LHCl adjusting pH is 4.5, add cellulase (the 2.5U/g root of kudzu vine) when the material temperature is 45 ℃, insulation is carried out enzyme and is handled 25min; As material temperature rise to 90 ℃ adding amylase boiling (the 10.0U/g root of kudzu vine) 50min, be cooled to 30 ℃ afterwards then, under aseptic condition, add saccharifying enzyme (the 65U/g root of kudzu vine), 0.25% dry yeast, 0.1% (NH simultaneously 4) 2SO 4, 0.05%KH 2PO 4, under 33 ℃ of conditions, ferment afterwards, get karusen distillation ethanol behind the 72h, measure ethanol content in the karusen.Obtain 0.089g ethanol/g root of kudzu vine, 6.767g ethanol/100g fermented product, starch utilization ratio are 93.2%.
Embodiment 6: get root of kudzu vine 30g, be cut into segment, rub broken section then into about 0.5-1.0cm, the root of kudzu vine and tap water are that 4: 1 ratio adds the tap water spice in mass parts, adding 6mol/LHCl adjusting pH is 4.5, add cellulase (the 1.5U/g root of kudzu vine) when the material temperature is 45 ℃, insulation is carried out enzyme and is handled 10min; As material temperature rise to 90 ℃ adding amylase boiling (the 5.0U/g root of kudzu vine) 40min, be cooled to 30 ℃ afterwards then, under aseptic condition, add saccharifying enzyme (the 65U/g root of kudzu vine), 0.15% dry yeast, 0.1% (NH simultaneously 4) 2SO 4, 0.1%KH 2PO 4, under 35 ℃ of conditions, ferment afterwards, get karusen distillation ethanol behind the 72h, measure ethanol content in the karusen.Obtain 0.085g ethanol/g root of kudzu vine, 6.230g ethanol/100g fermented product, starch utilization ratio are 94.2%.
Embodiment 7: get root of kudzu vine 30g, be cut into segment, rub broken section then into about 0.5-1.0cm, the root of kudzu vine and tap water are that 5: 1 ratio adds the tap water spice in mass parts, adding 6mol/LHCl adjusting pH is 4.5, add cellulase (the 3.5U/g root of kudzu vine) when the material temperature is 45 ℃, insulation is carried out enzyme and is handled 10min; As material temperature rise to 90 ℃ adding amylase boiling (the 5.0U/g root of kudzu vine) 40min, be cooled to 30 ℃ afterwards then, under aseptic condition, add saccharifying enzyme (the 100U/g root of kudzu vine), 0.25% dry yeast, 0.15% (NH simultaneously 4) 2SO 4, 0.1%KH 2PO 4, under 35 ℃ of conditions, ferment afterwards, get karusen distillation ethanol behind the 72h, measure ethanol content in the karusen.Obtain 0.090g ethanol/g root of kudzu vine, 6.468g ethanol/100g fermented product, starch utilization ratio are 94.2%.
Embodiment 8: get root of kudzu vine 30g, be cut into segment, rub broken section then into about 0.5-1.0cm, the root of kudzu vine and tap water are that 2: 1 ratio adds the tap water spice in mass parts, adding 6mol/LHCl adjusting pH is 4.5, add cellulase (the 1.5U/g root of kudzu vine) when the material temperature is 45 ℃, insulation is carried out enzyme and is handled 10min; As material temperature rise to 90 ℃ adding amylase boiling (the 5.0U/g root of kudzu vine) 40min, be cooled to 30 ℃ afterwards then, under aseptic condition, add saccharifying enzyme (the 65U/g root of kudzu vine), 0.25% dry yeast, 0.05% (NH simultaneously 4) 2SO 4, 0.2%KH 2PO 4, under 35 ℃ of conditions, ferment afterwards, get karusen distillation ethanol behind the 72h, measure ethanol content in the karusen.Obtain 0.061g ethanol/g root of kudzu vine, 4.576g ethanol/100g fermented product, starch utilization ratio are 93.6%.

Claims (5)

1. the method for a producing fuel ethanol by kudzu root synchronization saccharification fermentation, its step is as follows:
1) fragmentation of raw material: the root of kudzu vine is broken into the broken root of kudzu vine section of 5～10mm;
2) spice, boiling and enzyme are handled:
The broken root of kudzu vine after rubbing is added tap water mix thoroughly, the tap water that is added and the proportion by weight of the broken root of kudzu vine are 1: 1～6; It is 4.0～4.5 that the HCl that adds concentration and be 6mol/L regulates pH, is warming up to 45～50 ℃, and the ratio that adds 0～3.5U in the broken root of kudzu vine of every gram adds cellulase and carries out enzyme and handle 10～25min; Be warming up to 70～90 ℃ again, the ratio that adds 2.0～10.0U in the broken root of kudzu vine of every gram adds 70～90 ℃ of following boiling 20～50min of α-Dian Fenmei;
3) simultaneous saccharification and fermentation:
Root of kudzu vine material after the boiling is cooled to 30～35 ℃, under aseptic condition, adds saccharifying enzyme, (NH simultaneously 4) 2SO 4, KH 2PO 4And yeast, under 30～35 ℃ of conditions, carrying out anaerobically fermenting 48～84h, the ethanol that fermentation produces is collected in distillation.
2. method according to claim 1, the add-on that it is characterized in that described saccharifying enzyme are that every Ke Gegen adds 20～100U saccharifying enzyme.
3. method according to claim 1 is characterized in that described (NH 4) 2SO 4Add-on be that per 100 gram roots of kudzu vine add 0.05～0.15g (NH 4) 2SO 4
4. method according to claim 1 is characterized in that described KH 2PO 4Add-on be that per 100 gram roots of kudzu vine add 0.05～0.2gKH 2PO 4
5. method according to claim 1 is characterized in that described zymic add-on is that per 100 gram roots of kudzu vine add 0.10～0.308 yeast.
CN200610114730A 2006-11-22 2006-11-22 Method for producing fuel ethanol by kudzu root synchronization saccharification fermentation Expired - Fee Related CN101191135B (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN200610114730A CN101191135B (en) 2006-11-22 2006-11-22 Method for producing fuel ethanol by kudzu root synchronization saccharification fermentation
US12/447,895 US8333999B2 (en) 2006-11-22 2007-07-27 Process for comprehensively utilizing steam exploded radix puerariae and device therefor
PCT/CN2007/002268 WO2008061418A1 (en) 2006-11-22 2007-07-27 A procedure and equipment thereof for comprehensive utilization of radix pueraria
AU2007324208A AU2007324208B2 (en) 2006-11-22 2007-07-27 A procedure and equipment thereof for comprehensive utilization of Radix pueraria

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CN200610114730A CN101191135B (en) 2006-11-22 2006-11-22 Method for producing fuel ethanol by kudzu root synchronization saccharification fermentation

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102181076A (en) * 2011-02-28 2011-09-14 中国科学院过程工程研究所 Method for cleanly preparing starch containing abundant flavones and comprehensively utilizing radix puerariae resource
CN106434768A (en) * 2016-12-17 2017-02-22 周世容 Method for preparing ethanol fermentation broth through fermentation of manganese alginate and lycoris radiate bulbs
CN106967564A (en) * 2017-05-27 2017-07-21 贵州省仁怀市茅台镇郑氏酒业有限公司 A kind of preparation method of kudzuvine root wine
CN110923267A (en) * 2018-09-19 2020-03-27 南京理工大学 Ethanol distillation and cellulose pretreatment integrated ethanol preparation process

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1216150C (en) * 2002-04-12 2005-08-24 中国农业大学 Method for producing alcohol by solid fermentation of stalks

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102181076A (en) * 2011-02-28 2011-09-14 中国科学院过程工程研究所 Method for cleanly preparing starch containing abundant flavones and comprehensively utilizing radix puerariae resource
CN102181076B (en) * 2011-02-28 2013-03-27 中国科学院过程工程研究所 Method for cleanly preparing starch containing abundant flavones and comprehensively utilizing radix puerariae resource
CN106434768A (en) * 2016-12-17 2017-02-22 周世容 Method for preparing ethanol fermentation broth through fermentation of manganese alginate and lycoris radiate bulbs
CN106967564A (en) * 2017-05-27 2017-07-21 贵州省仁怀市茅台镇郑氏酒业有限公司 A kind of preparation method of kudzuvine root wine
CN110923267A (en) * 2018-09-19 2020-03-27 南京理工大学 Ethanol distillation and cellulose pretreatment integrated ethanol preparation process
CN110923267B (en) * 2018-09-19 2023-08-18 南京理工大学 Ethanol preparation process integrating ethanol distillation and cellulose pretreatment

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