CN101183073B - Quality controlled microparticles, preparation thereof and uses thereof - Google Patents
Quality controlled microparticles, preparation thereof and uses thereof Download PDFInfo
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Abstract
The present invention relates to quality control reagent and a quality control method of an instrument, which discloses a quality-control particle. The quality-control particle is the luminous particle of a protein coating marked by a biotin. The present invention also provides a preparation method of the quality-control particle and a quality control method using the quality-control particle. The quality-control particle and the quality control method disclosed by the present invention can be used for the performance test of a light-stimulating chemistry-luminescence instrument and for quality control and instrument calibration in a routine laboratory.
Description
Technical field
The present invention relates to the quality control reagent and the method for instrument.More particularly relate to quality control reagent, its preparation and method of quality control based on the analytical instrument of light-induced chemiluminescent.
Background technology
The light-induced chemiluminescent instrument can be widely used in the repercussion study of biomolecule, is mainly used in aspects such as medical diagnosis on disease clinically.The light-induced chemiluminescent technology is a kind of chemiluminescence based on high molecular particle, and it can the combination in the certain distance scope by photosensitive particulate and luminous particle, produces the ion-oxygen NE BY ENERGY TRANSFER, sends light signal, thereby testing sample is detected.Photosensitive particulate is the high molecular particle that is filled with Photoactive compounds, and luminous particle is the high molecular particle that is filled with luminophor and lanthanide series.The central principle of light-induced chemiluminescent technology is the microinvasion of energy, and the medium of transfer is the ion-oxygen of high-energy state.When red laser (670～690nm) irradiation photosensitive particulates after, discharge singlet oxygen ion (4 μ s), its propagation distance is about 200nm, have only under the enough approaching situation of the distance of photosensitive particulate and luminous particle, the singlet oxygen ion that photosensitive particulate discharges could arrive luminous particle, thereby generation series of chemical, launch the light of 520～620nm high level, because in the reaction system, the concentration of particulate is very low, collision probability is very little, therefore background signal is faint, after having only photosensitive particulate and luminous particle by the immune response combination, just can launch tangible light, so system sensitivity is very high.In medical diagnosis on disease, detecting pattern commonly used comprises three to four components: the photosensitive particulate of the antigen of the luminous particle of envelope antigen or antibody, biotin or digoxigenin labeled or antibody, Avidin or anti digoxin antibody bag quilt, in and antigen or antibody etc.More than each component and determined antigen or antibody combine by above incubation reaction of two steps, thereby by the power of chemiluminescence amount testing sample is carried out qualitative or detection by quantitative.With respect to traditional enzyme-linked immune analytic method, it has homogeneous phase (flushing-free), characteristics such as highly sensitive and easy and simple to handle, and easily is automated, so its application prospect is very wide.
At present, the light-induced chemiluminescent instrument is the same with general immunoassay instrument, all adopt quality controlled serum to carry out Quality Control, on the one hand owing to relate to bioactivator, therefore be difficult for a large amount of the acquisition, cause it to cost an arm and a leg, and need provide corresponding matched reagent, be not suitable for aspects such as instrument factory inspection, calibration at this quality controlled serum; Adopt quality controlled serum on the other hand, operation steps is loaded down with trivial details relatively, easier introduction personal error, and need above incubation reaction of two steps, be unfavorable for the step that simplifies the operation.In order to keep the biologically active of quality controlled serum, often need its freezing preservation, this storage transportation for reagent all has higher requirement.
In addition, quality controlled serum often adopts humanized's sample mix, differs greatly between criticizing and criticizing, and after changing lot number, need carry out statistical study again, is unfavorable for steady in a long-term the observation.
The present invention has carried out deep research at above problem just, has developed the quality controlled microparticles that does not need quality controlled serum to participate in, and has simplified the instrument quality control method, and the storage transportation is simple relatively, and is convenient to produce the repeatability between easier realization is criticized in batches.
Summary of the invention
The purpose of this invention is to provide a kind of novel quality controlled microparticles, its application, and a kind of novel quality control method that uses this quality controlled microparticles.
Principle of the present invention:
Utilize the light-induced chemiluminescent know-why, at luminous particle expoeridium biotin, photosensitive particulate expoeridium antibiotin by light-induced chemiluminescent mechanism, is estimated the power that produces light signal, thereby realizes the quality control of instrument.
Quality Control pattern of the present invention different with existing disease detection working mode figure (referring to Fig. 1):
In the medical diagnosis on disease pattern, reagent component is relatively complicated, comprise three or three above components, with the sandwich method is example, reagent generally comprises three components of photosensitive particulate of the luminous particle of antibody sandwich, biotin labeled antibody, antibiotin bag quilt, above-mentioned three components and determined antigen connect into an integral body by immune response, by light-induced chemiluminescent mechanism, produce light signal, by the signal power testing sample is carried out qualitative or quantitative, entire reaction generally need two the step above incubation.
In Quality Control pattern of the present invention, quality controlled microparticles has the luminous particle of biotin for the surface, can directly follow the photosensitive particulate combination of antibiotin bag quilt, compare with diagnostic mode, reaction conditions is fairly simple, do not need biological sample to participate in yet, and only react and a Buwen to educate, because the minimizing system that makes of component is more prone to control.
Technical scheme of the present invention:
One aspect of the present invention discloses a kind of quality controlled microparticles, is the luminous particle of albumen bag quilt, and wherein, above-mentioned albumen is by biotin labeling.This quality controlled microparticles with pan coating after the photosensitive particulate of antibiotin combines, under 670～690nm optical excitation, can launch 520～620nm wavelength photon.
Above-mentioned luminous particle is meant the high molecular particle that is filled with luminophor and lanthanide compound.Luminophor can be the derivant of Dioxene (dioxine) or thioxene (thioxene) etc., and lanthanide compound can be Eu (TTA)
3/ TOPO or Eu (TTA)
3/ Phen etc.
Above-mentioned photosensitive particulate is the high molecular particle that is filled with Photoactive compounds.Under 670～690nm optical excitation, can produce the singlet oxygen ion, under the enough near situation of itself and luminous particle distance, the single line oxonium ion is delivered to luminous particle, with the luminophor reaction in the luminous particle, produce ultraviolet light, ultraviolet light further excites lanthanide compound again, produces 520～620nm wavelength photon.Photoactive compounds can be a phthalocyanine dye etc.
Preferably, above-mentioned luminous particle is aldehyde group modified luminous particle.
A second aspect of the present invention discloses the preparation method of above-mentioned quality controlled microparticles, comprises the following steps:
C, use biotinylated protein;
D, with biotin labeled albumen bag by aldehyde group modified luminous particle.
Biotinylated protein is the biotinylated protein mode of present technique field routine, and biotin labeled albumen bag can be adopted the mode of antibody sandwich luminous particle conventional in this area to carry out by aldehyde group modified luminous particle.
By regulating biotin and protein ratio, obtain the different albumen of mark ratio, the mark ratio can not wait from 1～20 biotin of each protein molecule surface indicia, and the quality controlled microparticles that different mark ratios obtains is different with the efficient of photosensitive particulate combination.Also can adjust the ratio of albumen and particulate, obtain wrapping, can reach similar effects equally by the different quality controlled microparticles of ratio.
According to principle of the present invention as can be known, reaction of the photosensitive particulate of quality controlled microparticles and expoeridium antibiotin and the luminous biotin that at all has been the quality controlled microparticles expoeridium, and with very little by biotin labeled kinds of proteins relation, therefore this by biotin labeled albumen can be any do not influence that biotin combines with antibiotin also can be by biotin labeled albumen, consider preferred cow's serum gamma globulin (BGG) or bSA (BSA) from the angle that reduces cost.For those skilled in the art, can expect easily that other can be substituted the cow's serum gamma globulin by biotin labeled albumen and prepare quality controlled microparticles.
Third aspect present invention provides the application of above-mentioned quality controlled microparticles in the quality control of light-induced chemiluminescent analyser.
Above-mentioned quality control comprises the aspects such as service check, Routine Test Lab quality control inspection and instrument calibration to instrument.
Fourth aspect present invention, a kind of light-induced chemiluminescent analyser Quality Control parameter detection method is disclosed, comprise the following steps: in the light-induced chemiluminescent reaction system, to add the above-mentioned quality controlled microparticles of suitable concn and the photosensitive particulate of antibiotin bag quilt, keeping under the constant situation of photosensitive particulate concentration, by regulating the concentration of quality controlled microparticles, temperature of reaction and reaction time, control what and the signal power of reacting the ballistic phonon number.
Fifth aspect present invention, a kind of method that detects light-induced chemiluminescent analyser maximum count rate is disclosed, be included in and detect the quality controlled microparticles that adds variable concentrations in the plate hole respectively, each concentration can be carried out repeatedly replication, quality controlled microparticles concentration increases progressively gradually, the photosensitive particulate that in above-mentioned plate hole, adds antibiotin bag quilt then respectively, behind the incubation, the unit interval reading, when raising along with quality controlled microparticles concentration, signal value no longer raises or when signal occurring and overflowing, this highest signal value is the maximum count rate of instrument divided by the unit interval.
Sixth aspect present invention, a kind of method that detects the repeatability of light-induced chemiluminescent analyser is disclosed, be included in and detect the quality controlled microparticles that adds same concentrations in the plate hole respectively, the photosensitive particulate that in above-mentioned each plate hole, adds the antibiotin bag quilt of same concentrations more respectively, laser intensity and reading duration are set, behind the incubation, the unit interval reading, record data also calculate the CV value.
Seventh aspect present invention, a kind of method that detects light-induced chemiluminescent analyser instrument linearity is disclosed, be included in and detect the quality controlled microparticles that adds variable concentrations in the plate hole, the photosensitive particulate that in each plate hole, adds the antibiotin bag quilt of same concentrations again, laser intensity and reading duration are set, behind the incubation, the unit interval reading calculates linearly dependent coefficient r value.
Eighth aspect present invention, a kind of method that detects crossing-over rate between light-induced chemiluminescent analyser hole is disclosed, be included in and detect the hole of selecting cross arrangement in the plate hole, the quality controlled microparticles that adds same concentrations, in above-mentioned each plate hole, add the photosensitive particulate of the antibiotin bag quilt of same concentrations more respectively, laser intensity and reading duration are set, behind the incubation, the unit interval reading calculates crossing-over rate between the hole.
Quality controlled microparticles disclosed by the invention and quality control method, service check, the Routine Test Lab quality control inspection that can be used for instrument, the isoparametric detection of maximum count rate, repeatability, linearity, slope and sensitivity as instrument, according to these parameters instrument is calibrated, make the instrument performance that can be consistent of dispatching from the factory, reduce because the sample detection difference that the difference between instrument causes helps quality assessment between the chamber.Size that can also be by light signal and reappearance judge whether instrument temperature and sample adding system be normal.Can adopt the mensuration of high signal value quality controlled microparticles, ortho position blank well and amphi position blank well signal value is come crossing-over rate between the hole of detecting instrument, whether validation instrument meets the specifications of quality.
Description of drawings
Fig. 1: the mode of operation diagram of disease detection and Quality Control
A: disease detection mode of operation
B: Quality Control mode of operation
1: the luminous particle of antibody sandwich
2: determined antigen
3: biotin labeled antibody
4: the photosensitive particulate of antibiotin bag quilt
5: be quality controlled microparticles.
Fig. 2: example 5 is measured crossing-over rate application of sample distribution plan between holes, and its distribution can be according to the respective change of how much carrying out of the size of plate and plate hole.Wherein: S represents high value sample (signal value 100～3,000,000 quality controlled microparticles and photosensitive particulate), and B1 is the ortho position blank well, and B2 is the amphi position blank well.
Embodiment
Below the present invention will be further elaborated by specific embodiment, but embodiment and non-limiting protection scope of the present invention.
Embodiment 1: the quality controlled microparticles preparation of biotin labeling gamma globulin (BGG) bag quilt:
(1) preparation of biotin labeled BGG
With NaCNBH O.1M
3BGG (available from Pel-Freez Biological) is mixed with 10mg/ml solution, adopt DMSO (dimethyl formamide) configuration Biotin-X-X-NHS (N-hydroxy-succinamide modified biological element, manufacturer: SIGMA, production number: B3295) solution is to 16.172mg/ml, the amount that adds 5.4ul Biotin-X-X-NHS according to 1mg antibody joins Biotin-X-X-NHS liquid in the BGG solution, mixes and places under 4 ℃ and spend the night.Adopt dialysis to remove free unreacted biotin, dislysate is biotin labelled antibody dialysis buffer liquid (0.1M Tris, 0.3M NaCl, 0.005M EDTA-Na-2H
2O, pH 8.0).Dialysis finishes, and the BCA method is measured protein concentration.
(2) adopt above-mentioned labelled protein bag by aldehyde group modified luminous particle
Biotinylated protein that adding in the aldehyde group modified luminous particle of 10mg (rich positive biotechnology (Shanghai) Co., Ltd.) dialyses among Tween-20 solution, the 2mg above-mentioned (1) of 6.4 μ l 20% obtains and the NaCNBH of 16 μ l
3(25mg/ml, the MES damping fluid preparation of 0.05M pH6.0, now with the current), adding 0.1M pH6.0 MES is 400 μ l to cumulative volume, 37 ° of lucifuges are hatched 48h.The Gly solution (75mg/ml, the MES damping fluid preparation of 0.05M pH6.0) that adds 40 μ l, 37 ° of lucifuge revolving reaction 2h.Add 250 μ l BSA (200mg/ml, the MES damping fluid preparation of 0.05M pH6.0) solution.37 ° of lucifuge revolving reaction 16h.Centrifugal 30 minutes of 4 ℃ of 13000g.Abandon supernatant, wash again twice with the MES damping fluid of 1ml pH6.0.HEPES damping fluid (50mM HEPES, 300mM NaCl, 25mMEDTA-Na-2H with 1ml 0.05M pH8.0
2O, 1.6%BSA, 0.1%Dextran, 0.1%Tween-20,0.3745%TritonX-405,0.01% gentamicin, 0.05%Proclin) suspension (its final concentration is 10mg/ml).
The quality controlled microparticles that bag is done is measured solid content, gets in a small amount and is diluted to 20 μ g/ml, and 30 μ l dilutions add 60 μ l40 μ g/ml photosensitive particulate (AlphaScreen
TMStreptavidin-coated Donor Beads, production number: 6760002S, manufacturer: Perkin Elmer), incubation 15min in LiCA HT light-induced chemiluminescent system (the rich positive Medical Instruments in Shanghai company limited), laser excitation 1s, reading 1s, light signal 〉=1800000 think that bag is by success.
(3) quality controlled microparticles working fluid preparation
According to need of work, adopt the HEPES damping fluid of 0.05M pH8.0 that suspension in (2) is diluted to respective concentration, packing is preserved.
Embodiment 2: the quality controlled microparticles preparation of biotin labeling bSA (BSA) bag quilt
(1) preparation of biotin labeling bSA (BSA)
Use 0.1M NaCNBH
3BSA (available from EQUITECH-BIO INC) is mixed with 10mg/ml solution, adopt DMSO (dimethyl formamide) configuration Biotin-X-X-NHS (N-hydroxy-succinamide modified biological element, manufacturer: SIGMA, production number: B3295) solution is to 16.172mg/ml, the amount that adds 13.5ul Biotin-X-X-NHS according to 1mg antibody joins Biotin-X-X-NHS liquid in the BSA solution, mixes and places under 4 ℃ and spend the night.Adopt dialysis to remove free unreacted biotin, dislysate is biotin labelled antibody dialysis buffer liquid (0.1M Tris, 0.3M NaCl, 0.005M EDTA-Na-2H
2O, pH 8.0).Dialysis finishes, and the BCA method is measured protein concentration.
(2) adopt above-mentioned labelled protein bag by aldehyde group modified luminous particle
(2) are same in method for coating and the example 1.
(3) quality controlled microparticles working fluid preparation
(3) are same in compound method and the example 1.
In like manner, can also adopt other albumen bags of biotin labeling to be prepared quality controlled microparticles by luminous particle.Following example we adopt the quality controlled microparticles of biotin labeling BGG bag quilt to carry out instrument detecting and Quality Control.
Embodiment 3: adopt quality controlled microparticles detecting instrument maximum count rate
The quality controlled microparticles that adds variable concentrations at instrument, each concentration can be carried out repeatedly replication, quality controlled microparticles concentration increases progressively gradually, the photosensitive particulate that in above-mentioned hole, adds antibiotin bag quilt then, incubation 15min, the unit interval reading is when raising along with quality controlled microparticles concentration, signal value no longer raises or signal occurs and overflows, and this highest signal value is the maximum count rate of instrument divided by the unit interval.
Adopt said method, the quality controlled microparticles that we adopt the bag of biotin labeling BGG in the example 1 quilt detects the maximum count rate of LiCA HT light-induced chemiluminescent system (the rich positive Medical Instruments in Shanghai company limited), quality controlled microparticles concentration is respectively 20,40,60,80 μ g/ml, every hole adds 30 μ l in microwell plate, each concentration 5 hole, automatically add 60 μ l, 40 μ g/ml photosensitive particulates by instrument then, at 37 ℃ of incubation 15min of instrument internal, laser excitation 1s is set, reading 1s writes down each hole signal value.After measured, each concentration signal average is respectively: 2181668,4354769,5135782,51552478, as can be seen after quality controlled microparticles concentration is elevated to 60 μ g/ml, instrument signal no longer improves from top data, and the maximum count rate that therefore can draw this instrument is＞5000000S
-1The photon counter that this result and instrument the adopt explanation of dispatching from the factory is consistent.
Embodiment 4: the repeatability that adopts the quality controlled microparticles detecting instrument
Select corresponding plate hole position, add the high, normal, basic concentration quality controlled microparticles of 30 μ l successively.After advancing plate, in each hole, add the photosensitive particulate (40 μ g/ml) of 60 μ l again, laser intensity and reading duration are set, incubation 15min, instrument brings into operation, and reads photon number.With the following formula of numerical value substitution, try to achieve the CV value.
We adopt AFP quality controlled serum and quality controlled microparticles to detect LiCA HT light-induced chemiluminescent system precision respectively.The AFP quality controlled serum divides high, normal, basic 3 concentration; Quality controlled microparticles also adopts 3 concentration, is respectively 20,5,1 μ g/ml.Two kinds of each concentration of method are respectively measured 20 holes, AFP quality controlled serum assay method is with reference to corresponding reagent box instructions (fetus alpha globulin detection kit (light-induced chemiluminescent method), rich positive biotechnology (Shanghai) Co., Ltd.), the quality controlled microparticles assay method is same with example 3.Calculate each concentration sample precision according to above-mentioned formula.The AFP quality controlled serum is high, normal, basic three concentration determination results be respectively: 3.25%, 3.58%, 3.47%; And adopt three concentration determination results of quality controlled microparticles to be respectively: 1.27%, 1.89%, 2.04%.Therefore compare with traditional quality controlled serum mode detection, quality controlled microparticles mode reagent composition and operation steps are simpler, and factors such as easier minimizing reagent and manual operation also more can reflect the truth of instrument.The result that we also adopt quality controlled microparticles to obtain as can be seen from above-mentioned detection is better than the quality controlled serum measurement result.
Above-mentioned experiment is only measured precision in the plate of instrument, adopt similar method we also can between the plate of instrument and day between repeatability investigate.
Embodiment 5: adopt quality controlled microparticles detecting instrument linearity
Select corresponding plate hole position, add the quality controlled microparticles of many concentration of 30 μ l successively.After advancing plate, in each hole, add the photosensitive particulate (40 μ g/ml) of 60 μ l again, laser intensity and reading duration are set, incubation 15min, instrument brings into operation, and reads photon number.With the concentration value is X, and optical signal value is Y, with the following formula of numerical value substitution, tries to achieve the linearly dependent coefficient r value of two groups of data.
We adopt five concentration quality controlled microparticles of 20,10,5,1,0.1 μ g/ml to detect LiCA HT light-induced chemiluminescent system linear, and assay method and example 3 are together.Record 5 concentration light signal averages and be respectively 2263479,1119518,634659,134023,13238, the linearly dependent coefficient r that calculates instrument concentration and signal is 0.999.
Embodiment 6: crossing-over rate between the hole of employing quality controlled microparticles detecting instrument
Adopt Fig. 1 arrangement mode to carry out application of sample.In the hole that indicates S, add 30 μ l quality controlled microparticles (20 μ g/ml), advance plate.In the hole that indicates S, add 60 μ l photosensitive particulates (40 μ g/ml) with instrument.Incubation 15min.Record indicates S, B1, each hole reading of B2.
Testing result in LiCA HT light-induced chemiluminescent system is:
Calculate that crossing-over rate is 1.54 * 10 between the hole of instrument
-5
Adopt AFP high concentration quality controlled serum to detect, method and example 4 obtain together
Crossing-over rate is 1.53 * 10 between the hole of instrument
-5
Two kinds of basically identicals as a result that method obtains.
Amount of reagent, concentration and reaction time etc. that above-mentioned example adds all can adjust accordingly according to instrument model and specification.
More than describe the present invention according to AD HOC, but self-evident distortion of those skilled in the art and improvement also all comprise within the scope of the invention.
Claims (11)
1. quality controlled microparticles, it is characterized in that, described quality controlled microparticles is the luminous particle of albumen bag quilt, wherein, described albumen is by biotin labeling, and described albumen is cow's serum gamma globulin or bSA, described luminous particle is aldehyde group modified luminous particle, luminous particle is meant the high molecular particle that is filled with luminophor and lanthanide compound, and luminophor is selected from one or more in dioxine or the thioxene, and lanthanide compound is selected from Eu (TTA)
3/ TOPO or Eu (TTA)
3/ Phen.
2. quality controlled microparticles according to claim 1, it is characterized in that, described quality controlled microparticles with pan coating after the photosensitive particulate of antibiotin combines, under 670～690nm optical excitation, launch 520～620nm wavelength photon, described photosensitive particulate is the high molecular particle that is filled with Photoactive compounds, and described Photoactive compounds is a phthalocyanine dye.
3. the preparation method of quality controlled microparticles as claimed in claim 1 or 2 comprises the following steps:
A, use biotinylated protein;
B, with biotin labeled albumen bag by aldehyde group modified luminous particle.
4. claim 1 or 2 described quality controlled microparticles are used for the quality control of light-induced chemiluminescent analyser.
5. as purposes as described in the claim 4, it is characterized in that described quality control comprises the service check of instrument and the arbitrary project in the Routine Test Lab quality control inspection.
6. as purposes as described in the claim 5, it is characterized in that described quality control is an instrument calibration.
7. light-induced chemiluminescent analyser Quality Control parameter detection method, comprise the following steps: in the light activation reaction system, to add the claim 1 of suitable concn or the photosensitive particulate of the antibiotin bag quilt described in 2 described quality controlled microparticles and the claim 2, keeping under the constant situation of photosensitive particulate concentration, by regulating the concentration of quality controlled microparticles, temperature of reaction and reaction time, control what and the signal power of reacting the ballistic phonon number.
8. method that detects light-induced chemiluminescent analyser maximum count rate, comprise the following steps: in detecting plate hole, to add respectively the claim 1 or the 2 described quality controlled microparticles of variable concentrations, each concentration is carried out repeatedly replication, quality controlled microparticles concentration increases progressively gradually, the photosensitive particulate that in above-mentioned plate hole, adds antibiotin bag quilt then respectively, behind the incubation, the unit interval reading, when raising along with quality controlled microparticles concentration, signal value no longer raises or when signal occurring and overflowing, this highest signal value is the maximum count rate of instrument divided by the unit interval.
9. method that detects light-induced chemiluminescent analyser repeatability, comprise the following steps: in detecting plate hole, to add respectively the claim 1 or the 2 described quality controlled microparticles of same concentrations, the photosensitive particulate that in above-mentioned each plate hole, adds the antibiotin bag quilt of same concentrations more respectively, laser intensity and reading duration are set, behind the incubation, the unit interval reading, record data also calculate the CV value, and wherein CV is the coefficient of variation.
10. method that detects light-induced chemiluminescent analyser instrument linearity, comprise the following steps: in detecting plate hole, to add the claim 1 or the 2 described quality controlled microparticles of variable concentrations, the photosensitive particulate that in each plate hole, adds the antibiotin bag quilt of same concentrations again, laser intensity and reading duration are set, behind the incubation, the unit interval reading calculates linearly dependent coefficient r value.
11. method that detects crossing-over rate between light-induced chemiluminescent analyser hole, comprise the following steps: in detecting plate hole, to select the hole of cross arrangement, the claim 1 or the 2 described quality controlled microparticles that add same concentrations, the photosensitive particulate that in above-mentioned each plate hole, adds the antibiotin bag quilt of same concentrations more respectively, laser intensity and reading duration are set, behind the incubation, the unit interval reading calculates crossing-over rate between the hole.
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| CN103175953B (en) * | 2011-12-26 | 2015-04-15 | 苏州新波生物技术有限公司 | Europium nanoparticle fluorescence quality control serum as well as preparation method and application thereof |
| US10124072B2 (en) * | 2013-09-18 | 2018-11-13 | Caliper Life Sciences, Inc. | In-vivo reactive species imaging |
| CN105044047A (en) * | 2015-06-09 | 2015-11-11 | 天津市南开医院 | Kit for detecting recombinant protein expression and using method thereof |
| CN106872686B (en) * | 2017-02-16 | 2019-04-05 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | The preservation liquid of time-resolved fluorescence microballoon label myoglobins antibody |
| CN109187484B (en) * | 2018-09-13 | 2020-12-04 | 广西师范大学 | Method for detecting biotin by using affinity reaction to regulate and control carbon dot catalysis SERS |
| CN109856094A (en) * | 2018-12-21 | 2019-06-07 | 深圳市金准生物医学工程有限公司 | A kind of function detecting method of fluorescence immunity analyzer |
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Address after: 200131 3rd and 5th floors, building 1, No.88 Cailun Road, Pudong New Area pilot Free Trade Zone, Shanghai Patentee after: Kemei Boyang diagnostic technology (Shanghai) Co.,Ltd. Address before: No. 88, No. two building five floor 201210 East Shanghai Zhangjiang High Tech Park of Pudong New Area Cailun Road Patentee before: BEYOND DIAGNOSTICS (SHANGHAI) Co.,Ltd. |
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