CN101172948A - Method for separation and purification of cajanine and pinostrobin from extract of cajanus cajan branches and leaves - Google Patents
Method for separation and purification of cajanine and pinostrobin from extract of cajanus cajan branches and leaves Download PDFInfo
- Publication number
- CN101172948A CN101172948A CNA2007101445360A CN200710144536A CN101172948A CN 101172948 A CN101172948 A CN 101172948A CN A2007101445360 A CNA2007101445360 A CN A2007101445360A CN 200710144536 A CN200710144536 A CN 200710144536A CN 101172948 A CN101172948 A CN 101172948A
- Authority
- CN
- China
- Prior art keywords
- extraction
- branches
- leaves
- pinostrobin
- technology
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for separating and purifying functional components of the traditional Chinese medicine, namely, legumin and pinostrobin; aiming at providing a method for easily, safely, economically and effectively extracting and acquiring monomeric compounds of legumin and pinostrobin through separating and purifying from branches and leaves of the dal. The invention has the technical proposal that the method comprises the steps as follows: the extractive ethanol matter is taken from the branches and the leaves of the dal as the raw material, the supersonic oscillating flocculating technology, the negative pressure cavitating and suspending liquid solidifying and extracting technology, the big-pore absorbing resin collecting technology, the positive phase silica gel middle pressure chromatographic separation technology and the recrystalling technology are adopted to produce refined dal element and pinostrobin with the purity being more than 95 percent. The extractive receives simple impurity removal and repurification. The invention which has the advantages of simple method, easy operation, small loss of the target compounds, high yield and high purity of the product is suitable for the industrialized application, and has great importance to the industrialized production.
Description
Technical field
The present invention relates to a kind of method of separation and purification functional ingredient from plant, specifically is separation and purification cajanin and Pinostrobin from pigeonpea branches and leaves ethanol extraction.
Background technology
Pigeonpea [Cajanus cajan (L.) Millsp.] is annual or perennial woody plant, evergreen shrubs for the pulse family pigeonpea belongs to.Having another name called pigeon beans, tree soya bean etc., is a kind of medication among the people.Effects such as refrigerant, the heat that disappears, detoxifcation, analgesic hemostatic, inhibiting bacteria and diminishing inflammation are arranged, (Liu Zhongqiu etc., 1998 such as wound, burn, infection, bedsore and antisepsis and anti-inflammation of being used for the treatment of among the people; Sun Shaomei etc., 1995).In addition, the Semen Cajani extract preparation in national expenditures such as India, Brazil, Cuba, Mexico, South Africa in disease (Abbiw DK., 1990 such as treatment flu, bronchitis, measles, dysentery, hepatitis, tetter, burn, ulcer, tumours; Duke JA.et al, 1994:Milliken W., 1997; Grover JK., 2002), evident in efficacy.The clinical prescription of China is used for the treatment of necrosis of femoral head and circulation system disease (Yuan Hao etc., 2004).
In the present known pigeonpea activeconstituents comprise apigenin, luteolin, Vitexina, Saponaretin, cajanin, Longistylin A, Longistylin C, Pinostrobin, Whitfield's ointment, β-Gu Zaichun, naringenin-4 ', 7-dme, heneicosane, heneicosane, Cajanol and some volatile oil composition (Lin Li etc., 1999; Christopher JCet al, 1982; Cheng Zhiqing etc., 1992).
Cajanin in the pigeonpea and Pinostrobin content are higher and active outstanding.Cajanin and other two kinds of stilbene compound prescriptions can suppress bone loss, and it is complete to keep trabecular bone structure; The class estrogen effect of cajanin has good medicinal DEVELOPMENT PROSPECT (Chen Dihua, Sun Lan etc., 2007).And Pinostrobin is a kind of stronger active natural flavone constituents (.2006 such as Liu Xiang) that has, has antibiotic (Bhamarapravati et al, 2006), anti-fragrance, antiproliferative isoreactivity (Jean-Christophe Le Bail, 2000), pharmacological research in recent years finds that it promptly has tangible physiologically active (Pan Yongfeng etc., 2005) in aspects such as (gasteremphraxis) to antiulcer agent, anti-inflammatory, pain relieving, stomach emptying.Although the activity of cajanin that obtains from natural phant and Pinostrobin is outstanding, but up to the present seldom about the report of its production process, have only in the Chinese patent (CN1666745A) " a kind of pharmaceutical composition that is used for the treatment of osteoporosis " and relate to cajanin and Pinostrobin extracting and purifying method, adopt traditional technique means but purify, through silica gel column chromatography repeatedly, process is very long, target compound easily loses, and the bigger organic solvent usage quantity of toxicity is big, is unfavorable for healthy production.In addition, the separation and purification that relates to Pinostrobin is traditional method (Zhou Lidong, 1999; Liu Wenxia, 2006).
In sum, in the process of utilizing plant milk extract separation and purification cajanin and Pinostrobin, main influence factor is ubiquity length consuming time in the sepn process, and the purpose compound runs off big, makes cost raise.Therefore, be necessary to seek the method for a kind of handy and safe economical and efficient ground separation and purification cajanin and Pinostrobin.The present invention is intended to set up a kind of method that combines by various modern separating and purifying technology means, realizes that simply quick, the few separation and purification of loss obtains cajanin and Pinostrobin
Summary of the invention
The object of the present invention is to provide a kind of ethanol extraction with wooden skill leaf is raw material, obtain cajanin and Pinostrobin monomer in a large number, fast by ultra-sonic oscillation flocculation technique, negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction technology, macroporous adsorbent resin beneficiation technologies, purification on normal-phase silica gel medium pressure column chromatography isolation technique and low temperature crystallization and recrystallization technology, this method muscle Dan Yihang, target compound loss are lacked.The objective of the invention is to reach by following scheme:
Add 50-60 ℃ of warm water ultra-sonic oscillation, static flocculation in pigeonpea branches and leaves 70～95% ethanol extractions, resultant thickness throw out obtains the product of cajanin and Pinostrobin respectively behind negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction, the enrichment of manhole resin method and positive brick glue medium pressure column chromatography, purity is greater than 80%, obtains purity greater than 95% pure product through low temperature crystallization and recrystallization.
Above-mentioned cajanin and Pinostrobin extracting and purifying method, it is characterized in that, get a certain amount of pigeonpea branches and leaves ethanol extraction, 50～60 ℃ of warm water that add 3～5 times of volumes, vibration flocculation 15～25min under 30～60KHz condition in the ultrasonic oscillation extraction device, static 10～15min, obtain the thickness throw out, with 0.04～0.06MPa negative pressure is that power carries out the negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction with ethyl acetate, extract 0.5～2% (l: g) that used solvent volume is an extract quality, extraction times is 3～5 times, solvent for use also comprises trichloromethane except that ethyl acetate, methylene dichloride, concentrated extract is to doing, the solid substance that obtains is with 20-40% ethanol suspendible, and being configured to feed concentration is 2～4mg/mL suspendible soup, simultaneously macroporous adsorbent resin is adopted wet method dress post, keep liquid level, by adsorption column, applied sample amount 0.5～1BV is 6～8mL/g resin per hour with flow velocity with the soup of suspendible, the PH4 condition was descended adsorption column.Impurity is washed with 30-40% ethanol 2BV in the absorption back, uses 75-85% ethanol 6～8BV desorb then.Collect desorbed solution, be concentrated into the dried solid substance that obtains.Taking by weighing quality in advance is 2.5～3 times silica gel of extract quality, adopts wet method dress post, and solvent for use also comprises ether, ends hexane except that sherwood oil.With a small amount of organic solvent dissolution of gained solid substance, the chromatographic silica gel of quality such as adding and extract is mixed thoroughly, is reduced pressure and revolve ten, pack into and end compression leg in the phase silica gel, successively with 10-20BV sherwood oil (60-90 ℃), sherwood oil: trichloromethane (10: 1), trichloromethane compress and wash in continuously and take off, collect elutriant, every part of 1/40～1/20BV.Flow point is collected in the TLC monitoring, merges same section, and developping agent is a sherwood oil: trichloromethane=5: 1～0: 1, ultraviolet wavelength are 254nm and 360nm.Obtain Pinostrobin and cajanin solution successively, the low temperature crystallization obtains product, obtains pure product behind the recrystallization.
The used macroporous adsorbent resin of the extracting and purifying method of cajanin and Pinostrobin is D101, NKA-9, NKA-2, H103 type etc. in the above-mentioned pigeonpea.
The extracting and purifying method used silica gel of cajanin and Pinostrobin is 300～800 orders in the above-mentioned pigeonpea.Silica gel medium pressure post moving phase comprises the different ratios gradient of (sherwood oil, ether, normal hexane)-(trichloromethane, trichloromethane, ethyl acetate, acetone, methyl alcohol).
The used solvent of recrystallization is sherwood oil, ether, normal hexane, methylene dichloride, trichloromethane, acetone, acetonitrile and methyl alcohol, and recrystallization solvent for use ratio is sherwood oil (or ether, normal hexane): trichloromethane (or methylene dichloride, acetone, acetonitrile, methyl alcohol) 50: 1～1: 1.The low temperature recrystallization temperature is-10 ℃～25 ℃.
Advantage of the present invention:
1. this method is simple, and the cycle is short, can realize large-scale industrialization production.
2. product yield height, purity height.
Description of drawings
Fig. 1 is the structure of cajanin
Fig. 2 is the structure of Pinostrobin
Specific embodiments
Embodiment 1
Take by weighing the ethanol extraction 300g of the dried branches and leaves of pigeonpea, add in 60 ℃ of warm water (1.5L), vibration flocculation 20min under the 30-60KHz condition in the ultrasonic extraction device, static 15min, get the thickness throw out, with the 0.05MPa negative pressure is that power carries out the negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction with the 4.5L ethyl acetate, concentrated extract, being configured to feed concentration with 30% ethanol is 4mg/mL suspendible soup, applied sample amount 0.8BV, flow velocity is for per hour 8mL/g resin, PH4 condition descended NKA-9 type macropore to cross adsorption resin column.Impurity is washed with 30% ethanol 2BV in the absorption back, uses 85% ethanol 8BV desorb then.Collect stripping liquid, be concentrated into dried back and dissolve with small amount of methanol, adding 300g order number is that the chromatographic silica gel of 500-800 is mixed thoroughly, 40 ℃ of evaporated under reduced pressure.Take by weighing the 500-800 order silica gel of 2.5～3 times of extract qualities in advance, adopt sherwood oil wet method dress post, during the silica gel of having mixed sample packed in the compression leg, successively with 15BV sherwood oil (60-90 ℃), sherwood oil: methylene dichloride (10: 1), trichloromethane compress and wash in continuously and take off, flow point is collected in the TLC monitoring, concentrate and obtain Pinostrobin and cajanin successively, the low temperature crystallization obtains product, obtain compound 1 behind the recrystallization and be Pinostrobin, 33.82mg, purity is 95%, compound 2 is the art legumin, 31.36mg, and purity is 96%.
Embodiment 2
Take by weighing the ethanol extraction 300g of the fresh branches and leaves of pigeonpea, add in 55 ℃ of warm water (2L), improve to swing in ultrasonic wave and get in the device vibration flocculation 20min under 30～60KHz condition, static 15min, get the thickness throw out, with the 0.06MPa negative pressure is that power carries out the negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction with trichloromethane, concentrated extract, being configured to feed concentration with 30% ethanol is 2mg/mL suspendible soup, applied sample amount 0.6BV, flow velocity is for per hour 8mL/g resin, PH4 condition descended NKA-2 type macropore to cross adsorption resin column.Impurity is washed with 30% ethanol in the absorption back, uses 85% ethanol 7BV desorb afterwards, collects stripping liquid, is concentrated into to do the back with the small amount of methanol dissolving, adds 300g order number for the 300-400 chromatographic silica gel is mixed thoroughly, 40 ℃ of evaporated under reduced pressure.Take by weighing the 300-400 order silica gel of 2.5～3 times of extract qualities in advance, adopt sherwood oil wet method dress post, the brick mucilage binding of having mixed sample is gone in the middle compression leg, successively with 13BV sherwood oil (60-90 ℃), sherwood oil: trichloromethane (10: 1), the continuously middle wash-out of trichloromethane, flow point is collected in the TLC monitoring, concentrate and obtain Pinostrobin and cajanin successively, the low temperature crystallization obtains product, obtain compound 1 behind the recrystallization and be Pinostrobin, 30.18mg, purity is 95%: compound 2 is cajanin, 28.63mg, and purity is 95%.
Claims (7)
1. the method for separation and purification cajanin and Pinostrobin from a pigeonpea branches and leaves extract, be primarily characterized in that: the ethanol extraction of pigeonpea branches and leaves adds 60 ℃ of warm water ultra-sonic oscillation flocculations, static, resultant throw out obtains the goods of cajanin and Pinostrobin respectively behind the liquid-solid extraction of ethyl acetate negative pressure-cavitation homogenous solid-liquid phase, the enrichment of macroporous adsorbent resin method and purification on normal-phase silica gel medium pressure column chromatography, obtain purity greater than 95% pure product through low temperature crystallization and recrystallization.
2. according to the described pigeonpea branches and leaves of claim 1 ethanol extraction, it is characterized in that: the pigeonpea branches and leaves are mainly derived from the fresh or dry branches and leaves of each kind pigeonpea plant, and extracting method comprises ultrasonic-assisted extraction, thermal backflow extraction, supercritical CO
2Extraction, microwave-assisted extraction etc., solvent for use is a 70-95% ethanol.
3. according to the described ultra-sonic oscillation flocculation technique of claim 1, it is characterized in that: the volume that adds 50～60 ℃ of warm water in the pigeonpea branches and leaves ethanol extraction is 3～5 times of extract quality, the ultra-sonic oscillation flocculation time is 15～25min, frequency is 30～60KHz, and be 10～15min rest time.Hyperacoustic concussion effect makes the bigger impurity stripping gradually of polarity that comprises in the thickness throw out.
4. according to the described negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction of claim 1 technology, it is characterized in that: the thickness throw out is carried out the negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction, solvent for use also comprises trichloromethane, methylene dichloride except that ethyl acetate, extract 0.5～2% (L: g) that used solvent volume is an extract quality, extraction times is 3～5 times, and used pressure is 0.04～0.06MPa.The gained solid substance is a target compound behind the organic phase recovery solvent.This method is to be power with the negative pressure, utilizes the cavitation effect that bubble produces, a kind of forced fluid solid extraction technology of turbulence effect.
5. according to the described macroporous resin enrichment technology of claim 1, it is characterized in that: used resin comprises D101, NKA-9, NKA-2, broad spectrum macroporous adsorbent resins such as H103, the macroporous resin adsorption step adopts wet method dress post in this method, keep liquid level, it is 2～4mg/mL suspendible soup that the resulting solid substance of negative pressure-cavitation homogenous solid-liquid phase liquid-solid extraction partly is mixed with feed concentration with 20～40% ethanolic solns, simultaneously macroporous adsorbent resin is adopted wet method dress post, keep liquid level, the soup of suspendible is passed through adsorption column, applied sample amount is 0.5～1BV, is 6～8mL/g resin per hour with flow velocity, the PH=4 condition was descended adsorption column.Impurity is washed with 30～40% ethanol, 2 BV in the absorption back, uses 6～8 BV desorbs of 75～85% ethanol then.Collect stripping liquid, be concentrated into driedly, the gained solid substance is a target compound.
6. according to the described purification on normal-phase silica gel medium pressure column chromatography of claim 1 technology, it is characterized in that: specimen in use is a gained solid substance behind the resin concentration, and used silica gel is 300～800 orders.Taking by weighing quality in advance is 2.5～3 times silica gel of sample size, adopts wet method dress post, and solvent for use also comprises ether, ends hexane except that sherwood oil.With a small amount of organic solvent dissolution of sample, the chromatographic silica gel of quality such as adding and medicinal extract is mixed thoroughly, reducing pressure is spin-dried for, pack in the pre-packed silicagel column, successively with 10～20BV sherwood oil (60-90 ℃), right oily ether: trichloromethane (10: 1), methylene dichloride compress and wash in continuously and take off, collect elutriant, every part of 1/40～1/20BV.Detect the elutriant composition with silica gel thin-layer chromatography, merge same section, developping agent is a sherwood oil: trichloromethane=0: 1～5: 1, ultraviolet wavelength are 254nm and 360nm.Silica gel medium pressure post moving phase comprises the different ratios gradient of (sherwood oil, ether, normal hexane)-(methylene dichloride, trichloromethane, ethyl acetate, acetone, methyl alcohol).
7. according to described described low temperature crystallization of claim 1 and recrystallization technology, it is characterized in that: the used solvent of recrystallization is sherwood oil, ether, normal hexane, methylene dichloride, trichloromethane, acetone, acetonitrile and methyl alcohol, recrystallization solvent for use ratio is sherwood oil (or ether, normal hexane): trichloromethane (or methylene dichloride, acetone, acetonitrile, methyl alcohol) 50: 1-1: 1, and the low temperature recrystallization temperature is-10 ℃～25 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101445360A CN101172948B (en) | 2007-11-01 | 2007-11-01 | Method for separation and purification of cajanine and pinostrobin from extract of cajanus cajan branches and leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101445360A CN101172948B (en) | 2007-11-01 | 2007-11-01 | Method for separation and purification of cajanine and pinostrobin from extract of cajanus cajan branches and leaves |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101172948A true CN101172948A (en) | 2008-05-07 |
| CN101172948B CN101172948B (en) | 2010-09-22 |
Family
ID=39421730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101445360A Active CN101172948B (en) | 2007-11-01 | 2007-11-01 | Method for separation and purification of cajanine and pinostrobin from extract of cajanus cajan branches and leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101172948B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101875648A (en) * | 2010-07-12 | 2010-11-03 | 马灵媛 | Method for extracting and purifying Pinostrobin from medicinal plants, and pharmaceutical preparation and application thereof |
| CN101485714B (en) * | 2009-03-03 | 2010-12-29 | 东北林业大学 | Cajanus cajan leaf extract and application of monomer ingredient thereof in preparing medicament for resisting gram-positive bacteria |
| CN102703612A (en) * | 2012-06-29 | 2012-10-03 | 东北林业大学 | Method for preparing glucose syrup by performing acidolysis on cellulose biomass by negative pressure mass transfer and diffusion dialysis membrane separation technology |
| CN102731460A (en) * | 2011-04-13 | 2012-10-17 | 东北林业大学 | Method for separating and purifying cajanol from pigeon pea rhizomes |
| CN103073530A (en) * | 2013-02-04 | 2013-05-01 | 河南中医学院 | Method fro preparing pinostrobin from lindera reflexa |
| CN103159621A (en) * | 2011-12-16 | 2013-06-19 | 东北林业大学 | Stilbene active component, preparation method and application thereof |
| CN105362259A (en) * | 2015-10-20 | 2016-03-02 | 浙江中医药大学 | Chinese walnut leaf extract pinostrobin having uric acid reducing effect and preparation method thereof and application |
| CN107879906A (en) * | 2018-01-16 | 2018-04-06 | 河南省中医药研究院 | Extract cajanin, Longistylin A, Longistylin C, Cajanuslactone A technique |
| CN108096369A (en) * | 2018-01-16 | 2018-06-01 | 河南省中医药研究院 | A kind of trophic nerve cell, the extraction process of anti-apolexis composition |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1190716B1 (en) * | 1999-06-18 | 2004-05-19 | Hao Yuan | The leaves of cajanus cajan(l.) millsp and extract, formulation and uses thereof |
| EP1281392A1 (en) * | 2001-08-02 | 2003-02-05 | Cognis France S.A. | Cosmetic or/and pharmaceutical compositions containing plant extracts |
| CN1294905C (en) * | 2004-03-12 | 2007-01-17 | 中国医学科学院药用植物研究所 | Drug composition for treating osteoporosis |
-
2007
- 2007-11-01 CN CN2007101445360A patent/CN101172948B/en active Active
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485714B (en) * | 2009-03-03 | 2010-12-29 | 东北林业大学 | Cajanus cajan leaf extract and application of monomer ingredient thereof in preparing medicament for resisting gram-positive bacteria |
| CN101875648B (en) * | 2010-07-12 | 2012-12-12 | 马灵媛 | Method for extracting and purifying Pinostrobin from medicinal plants, and pharmaceutical preparation and application thereof |
| CN101875648A (en) * | 2010-07-12 | 2010-11-03 | 马灵媛 | Method for extracting and purifying Pinostrobin from medicinal plants, and pharmaceutical preparation and application thereof |
| CN102731460A (en) * | 2011-04-13 | 2012-10-17 | 东北林业大学 | Method for separating and purifying cajanol from pigeon pea rhizomes |
| CN103159621A (en) * | 2011-12-16 | 2013-06-19 | 东北林业大学 | Stilbene active component, preparation method and application thereof |
| CN103159621B (en) * | 2011-12-16 | 2015-04-15 | 东北林业大学 | Stilbene active component, preparation method and application thereof |
| CN102703612A (en) * | 2012-06-29 | 2012-10-03 | 东北林业大学 | Method for preparing glucose syrup by performing acidolysis on cellulose biomass by negative pressure mass transfer and diffusion dialysis membrane separation technology |
| CN103073530A (en) * | 2013-02-04 | 2013-05-01 | 河南中医学院 | Method fro preparing pinostrobin from lindera reflexa |
| CN103073530B (en) * | 2013-02-04 | 2014-07-23 | 河南中医学院 | Method for preparing pinostrobin from lindera reflexa |
| CN105362259A (en) * | 2015-10-20 | 2016-03-02 | 浙江中医药大学 | Chinese walnut leaf extract pinostrobin having uric acid reducing effect and preparation method thereof and application |
| CN105362259B (en) * | 2015-10-20 | 2018-06-15 | 浙江中医药大学 | A kind of Hickory Leaves extract pinostrobin with anti-trioxypurine effect and its preparation method and application |
| CN107879906A (en) * | 2018-01-16 | 2018-04-06 | 河南省中医药研究院 | Extract cajanin, Longistylin A, Longistylin C, Cajanuslactone A technique |
| CN108096369A (en) * | 2018-01-16 | 2018-06-01 | 河南省中医药研究院 | A kind of trophic nerve cell, the extraction process of anti-apolexis composition |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101172948B (en) | 2010-09-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101172948B (en) | Method for separation and purification of cajanine and pinostrobin from extract of cajanus cajan branches and leaves | |
| CN102816066B (en) | Method for extracting chlorogenic acid and hyperoside from lonicera japonica leaves | |
| CN101497594B (en) | Method for extracting, separating and purifying genistein from pigeon pea rhizome | |
| CN101186572B (en) | Method for separating and purifying salvianolic acid from red sage root liquid extract by one step | |
| CN102351819B (en) | Extraction, purification and preparation method of high-purity salvianolic acid B | |
| CN101260131A (en) | Method for extracting iridoid active site and monomer from eucommia bark | |
| CN103773820A (en) | Method for extracting, separating and purifying isoflavone active components biochanin A and genistein from dalbergia odorifera T.Chen leaves | |
| CN102746362A (en) | Method for extracting refined astragaloside from astragaliradix | |
| CN101172991B (en) | Method for extracting and purifying vitexin and isoviextin from cajanus cajan branches and leaves | |
| CN108864217A (en) | A kind of purification process of granatum punicalagins | |
| CN102731592B (en) | A kind of method extracting oleuropein and Tridemethylsciadopitysin from olive leaf | |
| CN107325138A (en) | A kind of method of the main anthocyanin of four kinds of extraction separation and purification in pomace from blackcurrant | |
| CN101348474A (en) | Method for preparing salvianolic acid B and tanshinol from Salvia miltiorrhiza stem | |
| CN104610401B (en) | A kind of method for extracting baicalin, baicalin and wogonin from Radix Scutellariae simultaneously | |
| CN101401843B (en) | Extracting method for active principle of traditional Chinese medicine red sage root | |
| CN103027937A (en) | Method for extracting general flavone from honeysuckle leaves | |
| CN103044504B (en) | A kind of method extracting Herba Boschniakiae Rossicae glucoside from Herba Boschniakiae Rossicae | |
| CN105175426B (en) | A kind of method of the extraction purification Bergenin from treebine stem | |
| CN103102255A (en) | Method for separating and purifying four phloroglucinol compounds from dryopteris fragrans | |
| CN103566195A (en) | Method for extracting alkaloid from dendrobium candidum | |
| CN102250174A (en) | Method for extracting jasminin from winter jasmine leaves | |
| CN205740840U (en) | A kind of isolated and purified device of high-purity silymarin | |
| CN106986904A (en) | A kind of Gastrodin extracted in the anesthesia stem from day and preparation method thereof | |
| CN107686502A (en) | A kind of preparation technology of chonglou saponin series | |
| CN107619427A (en) | A kind of method of the extraction purification rhizoma paridis saponin I from Paris polyphylla |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |