CN101168000A - Method for preparing medicine for treating pulmonary tuberculosis and quality standard detecting method - Google Patents
Method for preparing medicine for treating pulmonary tuberculosis and quality standard detecting method Download PDFInfo
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Abstract
The invention discloses a process for preparing and testing quality level of medicaments for curing tuberculosis. The prescription is composed of radix salvia miltiorrhiza, and baical skullcap root according to a certain weight ratio to make into pelletized granule. The method for testing quality level includes the discriminate approaches of radix salvia miltiorrhiza, and baical skullcap root, and the content has the content testing method of baicalin. The invention is used to cure tuberculosis, and has the advantages of good curing effect and controllable quality.
Description
Technical field
The present invention relates to medicament preparation method and quality standard detecting method, particularly a kind of reed mentioned in ancient books portion folk prescription process for producing granula and quality standard detecting method belong to field of medicaments.
Background technology
Tuberculosis is a kind of ancient disease, is the infectious disease of serious harm human health, is one of major disease of China's emphasis control, also is public health problem and the social problem that the whole world is paid close attention to.In recent years the hurried of resistant tuberculosis increases, and makes tuberculosis epidemic situation rebound significantly, and national stream in 2000 is raised wages and expected to show that initial drug resistant rates is 18.6%, wherein to two or more line antituberculosis drugs drug resistance persons nearly 60%; The acquired drug-resistance rate is 46.5%; Anti-multiple medicines tuberculosis (MDR.TB) resistant rate is 10.7%, wherein initial drug resistant rates is 7.6%, the acquired drug-resistance rate is 17.1%, estimate among the 2000000 bacterium sun consumptive that nearly 1/4 is the drug resistance case, a large amount of " obstinate " of Chan Shenging therefrom, chronic infection sexually transmitted disease (STD) people to various antituberculosis drugs thing drug resistances, long-term discharge of bacteria has brought great misery and threat to patient, family and society.
At present, be used for antiphthisic chemical medicine clinically and have only 12 kinds, wherein a line medicine isoniazid, rifampicin, pyrazinamide, ethambutol all have tangible liver toxicity, strepto-have tangible nephrotoxicity and the 8th pair of cranial nerve infringement, the two wires medicine has tangible gastrointestinal reaction or nephrotoxicity, liver toxicity more, and the necessary drug combination of antituberculosis therapy, list easily causes bacterial resistance with one or two kinds of medicines, causes the tuberculosis refractory.The full medicine that kills, isoniazid, rifampicin share the increase liver toxicity.
Along with the extensive use of antitubercular agent, the toxic and side effects of multi-drug resistant bacteria and medicine has become the main cause of chemotherapy failure, and quite a few lunger's focus is worsened repeatedly, forms refractory pulmonary tuberculosis.A large amount of Chinese medicines studies show that, in chemotherapy, cooperate Chinese medicine, and all advantages such as the compliance that increases the patient, the immunocompetence that improves the patient and medicine sterilization, fungistatic effect, reduction toxicity, the drug-fast incidence rate of reduction are arranged.It is significant to improving phthisical cure rate to sum up the phthisical experience of popularization Chinese medicine prevention.As far back as the fifties, Shao Changrong finds in treatment " consumptive disease ", the agent of many report utilization nourishing YIN and moistening the lung, but curative effect is undesirable, the expectorant bacterium often can not be turned out cloudy, he has searched a large amount of ancient literatures and has selected for use the Radix Scutellariae lung heat clearing to let out fire, in conjunction with the cough-relieving of Radix Stemonae parasite killing, add Radix Salviae Miltiorrhizae again and can improve perilesional blood circulation, eliminating blood stasis to promote regeneration of blood helps drug osmotic, thereby formulated " a kind of reed mentioned in ancient books portion folk prescription ", and make the red sheet of a kind of reed mentioned in ancient books portion and be applied to clinically, found that this medicine except making part tuberculosis patient expectorant bacterium turns out cloudy, yin deficiency syndrome also is improved.
The preparation technology of open " the red sheet of a kind of reed mentioned in ancient books portion " is a patent of invention, phthisical medicine of a kind of treatment and preparation method thereof, application number 200510028635.3, preparation method is Radix Stemonae 10-30 part, Radix Scutellariae 5-15 part, Radix Salviae Miltiorrhizae 6-18 part, the Radix Stemonae gets total alkaloids of sessile stemona root with ethanol extraction, salt acid treatment, and Radix Scutellariae extracts baicalin, Radix Salviae Miltiorrhizae with alcohol extraction after, use water extraction, the macroporous resin treatment water extract.The defective of this technology is: contain resisting pathogenic microbes composition and the Radix Stemonae alkaloid that antitussive, phlegm-dispelling functions are arranged in the Radix Stemonae, this technology is only extracted total alkaloids of sessile stemona root, has lost the resisting pathogenic microbes composition, and then has reduced the phthisical curative effect of treatment; Macroporous resin treatment Radix Salviae Miltiorrhizae water extract can make macroporous resin skeleton residue such as benzene,toluene,xylene, styrene, alkanes, diethylbenzene class organic residues such as (divinyl) introduce in the extract, produces the toxicity to nervous system, bone marrow etc.; This complex process cost height.
Prior art does not also have the quality standard of " a kind of reed mentioned in ancient books portion folk prescription " preparation, can't effectively guarantee product quality in producing and using, and then pharmaceutical effectiveness can not get guaranteeing.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of preparation method of treatment phthisical " a kind of reed mentioned in ancient books portion folk prescription " granule is provided.This method technology advantages of simple, reservation effective ingredient that can be more does not use macroporous resin, and no macroporous resin organic residue is introduced, and production cost is low.
The quality standard detecting method of another object of the present invention provides a kind of " a kind of reed mentioned in ancient books portion folk prescription " preparation makes " a kind of reed mentioned in ancient books portion folk prescription " preparation effectively guarantee product quality in producing and using, and then guarantees pharmaceutical effectiveness.
The preparation method of a kind of treatment of the object of the invention phthisical " a kind of reed mentioned in ancient books portion folk prescription " granule is achieved in that
Drug weight proportioning of the present invention is:
Radix Salviae Miltiorrhizae 300-400 part, Radix Stemonae 700-800 part, Radix Scutellariae 300-400 part
Be preferably
375 parts of Radix Salviae Miltiorrhizaes, 750 parts of the Radixs Stemonae, 375 parts of Radix Scutellariaes
The step preparation by the following method of the present invention's " a kind of reed mentioned in ancient books portion folk prescription " preparation:
(1) Radix Salviae Miltiorrhizae adds 7 times of amount alcohol reflux 2 hours, reclaims ethanol, is condensed into thick paste.
(2) Radix Salviae Miltiorrhizae decoction dregs and the Radix Stemonae, Radix Scutellariae decoct with water secondary, add 12 times of water gagings for the first time, decoct 1.5 hours, add 10 times of water gagings for the second time, decoct 1 hour, and collecting decoction filters, and is condensed into thick paste.
(3) combining step (1), step (2) thick paste, drying is pulverized, and powder gets dry extract.
(4) molding.Get step (3) gained dried cream powder and add adjuvant commonly used in the galenic pharmacy, make granule with process for producing granula commonly used in the galenic pharmacy.
In the above each component, weight is calculated with crude drug, and in the present invention, if weight is unit with the gram, this composition can be made into granule 1000 grams.
More than each form to be by weight as proportioning, when producing, can increase or reduce according to corresponding ratio, can be unit with kilogram or with the ton as large-scale production, weight can increase or reduce, but the crude drug weight proportion constant rate between the each component.
The present invention is improved by " a kind of reed mentioned in ancient books portion folk prescription " and gets function clearing heat and moistening lung, the anti-consumptive disease of invigorating blood circulation.Cure mainly the pulmonary tuberculosis folder blood stasis of holding concurrently, hectic fever, cough, chest pain is as thorn, or squamous and dry skin in the heart, darkish face and eyes, dark tongue quality or ecchymosis is arranged, the deep and hesitant pulse person.Also be used for tuberculosis of skin, the tuberculosis of bone and joint, scrofula.
The beneficial effect of a kind of treatment of the present invention phthisical medicine " a kind of reed mentioned in ancient books portion folk prescription " preparation method of granules is: use water extraction, meeting traditional Chinese medical science tradition fries in shallow oil soup and takes custom, has tcm characteristic, the Radix Stemonae has promptly kept total alkaloids of sessile stemona root, do not lose the resisting pathogenic microbes composition yet, and then can improve the phthisical curative effect of treatment; Without macroporous resin treatment, can avoid macroporous resin skeleton residue such as benzene,toluene,xylene, styrene, alkanes, diethylbenzene class organic residues such as (divinyl) to introduce in the extract, and then the toxicity of avoiding macroporous resin skeleton organic residue to produce; The simple cost of this technology is low.
The quality standard detecting method of another object of the present invention provides a kind of " a kind of reed mentioned in ancient books portion folk prescription " preparation is achieved in that
[character] this product be shallow palm fibre to the dark brown red granule, bitter in the mouth, little sweet.
This product 5g is got in [discriminating] (1), and porphyrize adds methanol 20ml, and supersound process 15 minutes filters, and filtrate is as need testing solution.Other gets Radix Scutellariae control medicinal material 1g, shines medical material solution in pairs with legal system.It is an amount of to get the baicalin reference substance again, adds methanol and makes the reference substance solution that every 1ml contains 0.5mg.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), drawing each 2 μ l of above-mentioned three kinds of solution puts respectively on same polyamide film, with acetic acid is developing solvent, and presaturation 30 minutes launches, take out, dry, spray is with 1% ferric chloride alcoholic solution, in the test sample chromatograph, with reference substance and the corresponding position of control medicinal material chromatograph on, show the principal spot of same color.
(2) get this product 5g, porphyrize, the 30ml that adds diethyl ether, supersound process 10 minutes filters, and filtrate volatilizes, and residue adds ethyl acetate 2ml makes dissolving, as need testing solution.Other gets Radix Salviae Miltiorrhizae control medicinal material 1g, shines medical material solution in pairs with legal system.Get Tanshinone I I again
AReference substance adds ethyl acetate and makes the reference substance solution that every 1ml contains 2mg.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, be developing solvent with cyclohexane extraction-ethyl acetate (6: 1), launch, take out, dry.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the principal spot of same color; With the corresponding position of reference substance chromatograph on, show identical kermesinus speckle.
(3) get this product 8g, porphyrize adds 70% ethanol 50ml, supersound process 30 minutes, filter, filtrate boils off ethanol, and residue adds strong ammonia solution and regulates pH value to 10～11, adds chloroform 20ml jolting again and extracts, divide and get chloroform layer, evaporate to dryness, residue add 1% hydrochloric acid solution 5ml makes dissolving, filters.Do two parts filtrate branch: drip bismuth potassium iodide test solution in the portion, generate salmon precipitation; Drip the silico-tungstic acid test solution in another part, generate milky white precipitate.
[inspection] should meet " relevant every regulation under Chinese pharmacopoeia granule of version in 2005 (appendix IC) item.
[assay] baicalin is according to " high effective liquid chromatography for measuring of Chinese pharmacopoeia version in 2005 (appendix VI D).
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With methanol-water-phosphoric acid (47: 53: 0.2) is mobile phase; The detection wavelength is 280nm.Number of theoretical plate should be not less than 2500 by the baicalin peak.
The preparation precision of reference substance solution takes by weighing at 4 hours baicalin reference substance of 60 ℃ of drying under reduced pressure an amount of, adds methanol and makes the solution that every 1ml contains 60ug, promptly.
This product under the content uniformity item is got in the preparation of need testing solution, and porphyrize is got about 0.5g, the accurate title, decide, and puts in the 50ml measuring bottle, adds 50% methanol, supersound process (power 250W, operating frequency 33KHZ) 20 minutes is put cold, be diluted to scale with 50% methanol, shake up, filter, precision is measured subsequent filtrate 5ml and is put in the 10ml measuring bottle, be diluted to scale with methanol, shake up, promptly.
Accurate respectively reference substance solution and each 10ul of need testing solution of drawing of algoscopy injects chromatograph of liquid, measures, promptly.
The every gram of this product contains Radix Scutellariae with baicalin (C
21H
18O
11) meter, must not be less than 9mg.
Above quality standard detecting method is the quality standard detecting method of " a kind of reed mentioned in ancient books portion folk prescription " granule, constant at prescription, the extraction process condition can guarantee again under the constant situation of the phthisical base substance of its Drug therapy, and it is suitable equally that the quality standard detecting method of the present invention's " a kind of reed mentioned in ancient books portion folk prescription " preparation is used for the check of other dosage form.As change tablet into, and it is the sheet of shallow palm fibre to dark brown red that the character item changes this product into, the inspection item changes into and should meet " relevant every regulation under Chinese pharmacopoeia tablet of version in 2005 (appendix ID) item.Other quality standard detecting method is constant.As change capsule into, it is granule and the powder of shallow palm fibre to dark brown red that character changes this product content into, check that item changes and should meet that " Chinese pharmacopoeia capsule of version in 2005 (appendix IG) is relevant every regulation down, and other quality standard detecting method changes into to be got this product content and get final product into.
For a better understanding of the present invention, below further set forth beneficial effect of the present invention by the test example.Test is intended to further specify effect of the present invention below, but not restriction of the present invention.
Test example one. the red granular mass standard determination method research of a kind of reed mentioned in ancient books portion of the present invention
[character] according to this product character description, this product is that light brown is to the dark brown red granule; Bitter in the mouth, little sweet.
[discriminating] formulated the discriminating of Radix Scutellariae, the Radix Stemonae, Radix Salviae Miltiorrhizae.
(1) thin layer of Radix Scutellariae is differentiated and is got this product 5g, and porphyrize adds methanol 20ml, and supersound process 15 minutes filters, and filtrate is as need testing solution.Other gets Radix Scutellariae control medicinal material 1g, shines medical material solution in pairs with legal system.It is an amount of to get the baicalin reference substance again, adds methanol and makes the reference substance solution that every 1ml contains 0.5mg.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), drawing each 2 μ l of above-mentioned three kinds of solution puts respectively on same polyamide film, with acetic acid is developing solvent, and presaturation 30 minutes launches, take out, dry, spray is with 1% ferric chloride alcoholic solution, in the test sample chromatograph, with reference substance and the corresponding position of control medicinal material chromatograph on, show the principal spot of same color.
(2) Tanshinone I I in the Radix Salviae Miltiorrhizae
AThin layer differentiate: get this product 5g, porphyrize, the 30ml that adds diethyl ether, supersound process 10 minutes filters, filtrate volatilizes, residue adds ethyl acetate 2ml makes dissolving, as need testing solution.Other gets Radix Salviae Miltiorrhizae control medicinal material 1g, shines medical material solution in pairs with legal system.Get Tanshinone I I again
AReference substance adds ethyl acetate and makes the reference substance solution that every 1ml contains 2mg.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, be developing solvent with cyclohexane extraction-ethyl acetate (6: 1), launch, take out, dry.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the principal spot of same color; With the corresponding position of reference substance chromatograph on, show identical kermesinus speckle.
(3) discriminating of the Radix Stemonae: get this product 8g, porphyrize adds 70% ethanol 50ml, supersound process 30 minutes, filter, filtrate boils off ethanol, and residue adds strong ammonia solution and regulates pH value to 10～11, adds chloroform 20ml jolting again and extracts, divide and get chloroform layer, evaporate to dryness, residue add 1% hydrochloric acid solution 5ml makes dissolving, filters.Do two parts filtrate branch: drip bismuth potassium iodide test solution in the portion, generate salmon precipitation; Drip the silico-tungstic acid test solution in another part, generate milky white precipitate.
[inspection] should meet every regulation relevant under the granule item (appendix IC of Chinese Pharmacopoeia version in 2005).
1 according to " an appendix IXF of Chinese pharmacopoeia version in 2005 arsenic salt inspection technique, first method is measured result＜2PPm to " the red granule of a kind of reed mentioned in ancient books portion " arsenic salt.Simultaneously according to " an appendix IXE of Chinese pharmacopoeia version in 2005 heavy metal inspection technique, second method is measured result＜10PPm to " the red granule of a kind of reed mentioned in ancient books portion " heavy metal.Therefore exclude quality standard.Test as follows.
1.1 arsenic salt inspection technique standard arsenic solution: precision takes by weighing 105 ℃ of arsenic trioxide 0.132g that are dried to constant weight, puts in the 1000ml measuring bottle, add 20% sodium hydroxide solution 5ml dissolving after, dilute sulfuric acid neutralization with an amount of adds dilute sulfuric acid 10ml again, is diluted with water to scale, shake up, as stock solution.
Before facing usefulness, precision is measured stock solution 10ml, puts in the 1000ml measuring bottle, adds dilute sulfuric acid 10ml and is diluted with water to scale, shakes up promptly to get (As that every 1ml is equivalent to 1.0ug).
The preparation of the preparation facilities of standard arsenic speckle: get lead acetate Cotton Gossypii an amount of (60-100mg) and be torn into loose shape, each a small amount of, to pack into equably among the airway C with thin Glass rod, it is appropriate that degree of tightness is wanted, and tubulature highly is 60-80mm.With 1 on Glass rod gripping mercuric bromide reagent paper (its size can cover D top bore and not expose outside the plane and be advisable), put on the plane, cock D top, cover the aperture, cover spiral cover E and screw.
Precision is measured standard arsenic solution 2ml, put in the A bottle, add hydrochloric acid 5ml and water 21ml, add 5 of the inferior stannum test solutions of potassium iodide test solution 5ml and acid chlorization again, after room temperature is placed 10 minutes, add zinc granule 2g, fill in bottle with ready airway C is close immediately in A, and the A bottle put in the 25--40 ℃ of water-bath reaction 45 minutes, take out the mercuric bromide reagent paper, promptly.
The preparation of test sample: get this product porphyrize, take by weighing 1g, the accurate title, decide, adding calcium hydroxide 1g mixes, and adds low amounts of water, stir evenly, dry back makes carbonization with little baked wheaten cake is bright earlier, blazingly makes complete ashing at 500～600 ℃ again, puts cold, add water 4ml, make dissolving, add hydrochloric acid 5ml again and make into 28ml in right amount, as need testing solution with water.Check in accordance with the law.
Inspection technique: get need testing solution, put in the A bottle, add 5 of the inferior stannum test solutions of potassium iodide 5ml and acid chlorization, after room temperature was placed 10 minutes, add zinc granule 2g, fill in bottle with ready airway C is close immediately in A, and the A bottle put in the 25--40 ℃ of water-bath reaction 45 minutes, take out the mercuric bromide reagent paper, arsenic speckle and the standard arsenic speckle that generates compared, promptly.
The result judges: the arsenic speckle that test liquid generates is more shallow than standard arsenic speckle color, is judged to up to specification.
1.2 the preparation of heavy metal inspection technique standard lead solution: precision takes by weighing plumbi nitras 0.160g, puts in the 1000ml measuring bottle, add nitric acid 5ml and water 50ml the dissolving after, be diluted with water to scale, shake up, it is standby to do stock solution.
Face with before, precision is measured stock solution 10ml and is put in the 100ml measuring bottle, thin up shakes up to scale, promptly gets (Pb that every 1ml is equivalent to 10 μ g).
This product porphyrize is got in the preparation of need testing solution, takes by weighing 1g, and accurate the title decides, put in the crucible of ignition to constant weight, slowly blazing carbonization is extremely fully put and is chilled to room temperature, adds sulphuric acid 1ml and makes moistening, after low-temperature heat to sulphuric acid eliminates, add nitric acid 0.5ml, evaporate to dryness is after eliminating to the nitrogen oxide steam, put cold, blazingly make complete ashing at 500-600 ℃, put coldly, add hydrochloric acid 2ml, put and add water 15ml in the water-bath behind the evaporate to dryness, drip ammonia solution to instructions phenolphthalein solution is shown neutral, add acetate buffer (PH3.5) 2ml again, after the slight fever dissolving, move in the nessler colorimetric tube, thin up becomes 25ml (second pipe); Other gets the reagent of preparation need testing solution, puts in the porcelain dish behind the evaporate to dryness, adds acetate buffer (PH3.5) 2ml, thin up becomes 15ml, after the slight fever dissolving, moves in the nessler colorimetric tube, add standard lead solution 1ml with pipet, thin up becomes 25ml (first pipe) again, checks in accordance with the law.
Inspection technique: add each 2ml of thioacetamide test solution respectively in above-mentioned two pipes, shake up, placed 2 minutes, with putting on the blank sheet of paper, from up to down have an X-rayed, color of showing in the second pipe and first pipe must not compare darker.
The result judges: color of showing in the second pipe and first pipe must not compare darker, contain the heavy metal amount less than 10ppm.The result is as follows for 3 batch sample heavy metals, arsenic salt test.
Table 13 batch sample heavy metal, arsenic salt test result
Table 23 batch sample testing result
[assay]
1, instrument and Tianjin, reagent island Lc-10ATVP type high performance liquid chromatograph; Kromassil C
18Chromatographic column 5 μ, 250 * 4.6mm, N-2000 dual pathways chromatographic work station; Kromassil C
18Chromatographic column 5 μ, 250 * 4.6mm, Anastar chromatographic work station.Baicalin reference substance (numbering 110715-200212, Nat'l Pharmaceutical ﹠ Biological Products Control Institute uses for assay).Used methanol is chromatographically pure.Other reagent is analytical pure.
2, specificity is tested the granule blank of getting baicalin reference substance, test sample (20060601) respectively and not containing Radix Scutellariae, measures according to method in the quality standard, and it is noiseless to the mensuration of this product to get HPLC collection of illustrative plates blank sample; Sample and baicalin reference substance have corresponding chromatographic peak on corresponding peak position, and separating degree is better.This method is simple to operate, and specificity is strong.
3, the design of extraction and determination method
According to the character of baicalin, selecting 70% ethanol, 50% methanol for use is solvent, adopts supersound extraction and reflux, extract, respectively, and quantity of solvent is made as 30ml, 40ml, 50ml, and the time was made as 10 minutes, 20 minutes, 30 minutes.Test as follows:
Get this product (lot number 20060601) 10g, porphyrize is got about 0.5g, and accurate the title decides, and adds coordinative solvent, extract, put coldly, be diluted to scale, shake up, filter with corresponding reagent, precision is measured subsequent filtrate 5ml and is put in the 10ml measuring bottle, is diluted to scale with corresponding reagent, shakes up, promptly.
Accurate respectively reference substance solution and each 10ul of need testing solution of drawing of algoscopy injects chromatograph of liquid, measures, promptly.
Measurement result table 4
| Numbering | Solvent | Extracting method | Content (mg/g) | |
| 1 | 70% ethanol | 50ml refluxed 3 hours | 12.89 | |
| 2 | 70 | Ultrasonic | 20 minutes of 50ml | 12.91 |
| 3 | 50 | Ultrasonic | 20 minutes of 50ml | 12.89 |
| 4 | 50 | Ultrasonic | 20 minutes of 30ml | 12.91 |
| 5 | 50 | Ultrasonic | 20 minutes of 40ml | 12.86 |
| 6 | 50% methanol | Ultrasonic 10 minutes of 50ml | 12.59 | |
| 7 | 50% methanol | Ultrasonic 30 minutes of 50ml | 12.87 |
The result shows that it is approaching that this product reflux, extract, and supersound extraction record content, so adopt supersound extraction; With 50% methanol than 70% ethanol extraction good separating effect, so select with 50% methanol extraction; Liquid volume added does not have obviously influence to measuring; Supersound extraction was extracted fully in 20 minutes, for guaranteeing to extract fully, reduced the experimental error choosing, so selection was with 50% methanol 50ml supersound extraction 30 minutes.
4, the investigation precision of linear relationship takes by weighing at 4 hours baicalin reference substance 10.31mg of 60 ℃ of drying under reduced pressure, puts in the 50ml measuring bottle, adds methanol to scale, in contrast product solution.Accurate draw above-mentioned reference substance solution 1,2,3,4,5,6ml puts respectively in the 10ml measuring bottle, adds methanol respectively to scale, its concentration is respectively 20.62,41.24,61.86,82.48,103.1,123.72mg/ml.Draw above-mentioned reference substance solution 10 μ l respectively and inject chromatograph of liquid, record the peak area integrated value.Concentration with the baicalin reference substance is abscissa, and the peak area integrated value is a vertical coordinate, carries out linear regression, gets regression equation: Y=59131x-4053.1, correlation coefficient r=0.9999.
The investigation of table 5 linear relationship
The result shows that the baicalin sample size between 0.2062～1.2372 μ g, is good linear relationship with the peak area integrated value.See Fig. 1
5, precision experiment
5.1 repeatability
The same need testing solution of accurate absorption, continuous sample introduction 6 times, the result is as follows:
Table 6 repeated experiment result
The result shows that the repeated experiment result is good.
5.2 middle precision
Get this product (lot number 20060601), adopt different experiments factor A, B to test respectively: A adopts Tianjin, 1# island Lc-10ATVP type high performance liquid chromatograph, Kromassil C by experiment people a
18Chromatographic column 5 μ, 250 * 4.6mm, N-2000 dual pathways chromatographic work station; B experiment people b adopts Tianjin, 2# island Lc-10ATVP type high performance liquid chromatograph, Kromassil C
18Chromatographic column 5 μ, 250 * 4.6mm, Anastar chromatographic work station.Press described method of text and condition extraction and determination, the result is as follows:
Precision experimental result in the middle of the table 7
The precision experimental result was good in the middle of the result showed.
6, stability test is got this product (lot number 20060601), press described method of text and condition extraction and determination, then once, record its baicalin peak area integrated value in 2 hours, 4 hours, 6 hours, 8 hours each sample introduction, the stability of investigation method the results are shown in Table 8.
Table 8 stability test result
The result shows that sample had good stable in 8 hours.
7, accuracy:
Precision takes by weighing test sample (lot number 20060601) 0.25g that predicts content, and accurate the title decided totally 6 parts, add baicalin reference substance 3.4mg respectively, the accurate title, decide, and puts in the 50ml measuring bottle, it is an amount of to add 50% methanol, ultrasonic 30 minutes, puts cold, add 50% methanol to scale, shake up, leave standstill, filter, the accurate subsequent filtrate 5ml that draws puts in the 10ml measuring bottle, adds methanol and is diluted to scale, shakes up promptly.Measure content, calculate recovery rate.The results are shown in Table 9.
The amount B of the contained tested composition of A sample adds pure product amount C measured value
Average recovery experimental result table 9
By above result as can be known, average recovery is good.
8, sample size measurement result
Table 10 assay result
Two clinical trials of the present invention of test example
Clinical data 64 routine secondary pulmonary tuberculosises are controlled the patient of bacterium sun again, are divided into 2 groups at random.Matched group 32 examples wherein, male 20 examples, women 12 examples; 21～72 years old age, average (46.5 ± 16.1) year; 32 examples are organized in treatment, male 22 examples, women 10 examples; 19～71 years old age, average (46.3 ± 16.3) year.Two groups of physical data are learned processing by statistics, and difference does not have significance (P＞0.05), has comparability.
The Therapeutic Method matched group is strengthened the phase chemotherapy regimen by controlling bacterium sun pulmonary tuberculosis again, single with the anti-consumptive disease treatment of Western medicine: 2H3R3Z3E3 ((isoniazid (H), rifampicin (R), pyrazinamide (Z), ethambutol (E), streptomycin (S), the next day 1 time, 2 totally months), drug dose: every mouth decoction being taken at a draught H 0.6g, R 0.6g, Z 2.0g, E 1.25g, the next day intramuscular injection S 0.75g.The treatment group adds the red granule therapy of a kind of reed mentioned in ancient books portion, an oral 15g, 2 times on the one on the treatment of control group basis.
Criterion of therapeutical effect
1. sputum conversion standard expectorant mycobacterium tuberculosis is checked the sputum conversion situation, the cloudy commentaries on classics: expectorant smear and expectorant are cultivated all negative; Depletion of YIN changes: expectorant smear or expectorant are cultivated and are positive.
2.X line improves criterion of therapeutical effect X line and changes situation, significantly absorb: focus absorbs more than or equal to 1/2 former focus; Absorb: focus absorbs less than 1/2 former focus; Constant: focus does not have significant change.
3 statistical methods: adopt X
2Check.
The result
1. two groups of sputum conversion situation treatment group sputum conversion 30 examples, depletion of YIN changes 2 examples, negative conversion rate 93.75% (30/32 example); Matched group sputum conversion 23 examples, depletion of YIN changes 10 examples, and comparing difference has significance (P＜0.05) between two groups of negative conversion rates 71.88% (23/32 example)
2. two groups of X line focus absorbing state treatment groups significantly absorb 5 examples, absorb 24 examples, constant 3 examples, total absorptivity 90.63% (29/32 example); Matched group significantly absorbs 2 examples, absorbs 15 examples, and constant 15 examples are sent out yield 53.13% (17/32 example) for total mouthful.Comparing difference has significance (P＜0.01) between two groups.
Description of drawings
Fig. 1 baicalin linear relationship chart
The specific embodiment
Below further specify the present invention by specific embodiment.
Embodiment 1 a kind of reed mentioned in ancient books portion folk prescription process for producing granula
Take by weighing Radix Salviae Miltiorrhizae 375g, Radix Stemonae 750g, Radix Scutellariae 375g.
Step preparation by the following method:
(1) Radix Salviae Miltiorrhizae adds 7 times of amount alcohol reflux 2 hours, reclaims ethanol, is condensed into thick paste.
(2) Radix Salviae Miltiorrhizae decoction dregs and the Radix Stemonae, Radix Scutellariae decoct with water secondary, add 12 times of water gagings for the first time, decoct 1.5 hours, add 10 times of water gagings for the second time, decoct 1 hour, and collecting decoction filters, and is condensed into thick paste.
(3) combining step (1), step (2) thick paste, drying is pulverized, and powder gets dry extract.
(4) molding.Get step (3) gained dried cream powder and add dextrin 240g, cane sugar powder, add the suitable quantity of water wet granulation in right amount to 1000g, oven dry below 80 ℃, packing is promptly.
Embodiment 2 a kind of reed mentioned in ancient books portion folk prescription process for producing granula
Radix Salviae Miltiorrhizae 300g, Radix Stemonae 600g, Radix Scutellariae 400g.
Step preparation by the following method:
(1) Radix Salviae Miltiorrhizae adds 7 times of amount alcohol reflux 2 hours, reclaims ethanol, is condensed into thick paste.
(2) Radix Salviae Miltiorrhizae decoction dregs and the Radix Stemonae, Radix Scutellariae decoct with water secondary, add 12 times of water gagings for the first time, decoct 1.5 hours, add 10 times of water gagings for the second time, decoct 1 hour, and collecting decoction filters, and is condensed into thick paste.
(3) combining step (1), step (2) thick paste, drying is pulverized, and powder gets dry extract.
(4) molding.Get step (3) gained dried cream powder and add dextrin 100g, saccharin sodium 2g, magnesium stearate 1-10g, cane sugar powder in right amount to 1000g, tabletting behind the mixing is ground into granule, and packing promptly.
Embodiment 3 a kind of reed mentioned in ancient books portion folk prescription process for producing granula
Take by weighing following traditional Chinese medicines
Radix Salviae Miltiorrhizae 400g, Radix Stemonae 700g, Radix Scutellariae 300g.
Step preparation by the following method:
(1) Radix Salviae Miltiorrhizae adds 7 times of amount alcohol reflux 2 hours, reclaims ethanol, is condensed into thick paste.
(2) Radix Salviae Miltiorrhizae decoction dregs and the Radix Stemonae, Radix Scutellariae decoct with water secondary, add 12 times of water gagings for the first time, decoct 1.5 hours, add 10 times of water gagings for the second time, decoct 1 hour, and collecting decoction filters, and is condensed into thick paste.
(3) combining step (1), step (2) thick paste, drying is pulverized, and powder gets dry extract.
(4) molding.Get step (3) gained dried cream powder and add lactose 320g, vanillin 3g, starch, add an amount of wet granulation of 90% ethanol in right amount to 1000g, oven dry below 80 ℃, packing is promptly.
Claims (5)
1. treat phthisical medicine for one kind, the prescription composition by the drug weight proportioning is: Radix Salviae Miltiorrhizae 300-400 part, Radix Stemonae 700-800 part, Radix Scutellariae 300-400 part is characterized in that step preparation by the following method:
(1) Radix Salviae Miltiorrhizae adds 7 times of amount alcohol reflux 2 hours, reclaims ethanol, is condensed into thick paste.
(2) Radix Salviae Miltiorrhizae decoction dregs and the Radix Stemonae, Radix Scutellariae decoct with water secondary, add 12 times of water gagings for the first time, decoct 1.5 hours, add 10 times of water gagings for the second time, decoct 1 hour, and collecting decoction filters, and is condensed into thick paste.
(3) combining step (1), step (2) thick paste, drying is pulverized, and powder gets dry extract.
(4) molding.Get step (3) gained dried cream powder and add adjuvant commonly used in the galenic pharmacy, make granule with process for producing granula commonly used in the galenic pharmacy.
2. treat phthisical medicinal granule quality standard detecting method for one kind, the prescription composition by the drug weight proportioning is: Radix Salviae Miltiorrhizae 300-400 part, Radix Stemonae 700-800 part, Radix Scutellariae 300-400 part is characterized in that comprising following method step:
[character] this product be shallow palm fibre to the dark brown red granule, bitter in the mouth, little sweet.
This product 5g is got in [discriminating] (1), and porphyrize adds methanol 20ml, and supersound process 15 minutes filters, and filtrate is as need testing solution.Other gets Radix Scutellariae control medicinal material 1g, shines medical material solution in pairs with legal system.It is an amount of to get the baicalin reference substance again, adds methanol and makes the reference substance solution that every 1ml contains 0.5mg.Test according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), drawing each 2 μ l of above-mentioned three kinds of solution puts respectively on same polyamide film, with acetic acid is developing solvent, and presaturation 30 minutes launches, take out, dry, spray is with 1% ferric chloride alcoholic solution, in the test sample chromatograph, with reference substance and the corresponding position of control medicinal material chromatograph on, show the principal spot of same color.
(2) get this product 5g, porphyrize, the 30ml that adds diethyl ether, supersound process 10 minutes filters, and filtrate volatilizes, and residue adds ethyl acetate 2ml makes dissolving, as need testing solution.Other gets Radix Salviae Miltiorrhizae control medicinal material 1g, shines medical material solution in pairs with legal system.Get Tanshinone I I again
AReference substance adds ethyl acetate and makes the reference substance solution that every 1ml contains 2mg.According to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B) test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, be developing solvent with cyclohexane extraction-ethyl acetate (6: 1), launch, take out, dry.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the principal spot of same color; With the corresponding position of reference substance chromatograph on, show identical kermesinus speckle.
(3) get this product 8g, porphyrize adds 70% ethanol 50ml, supersound process 30 minutes, filter, filtrate boils off ethanol, and residue adds strong ammonia solution and regulates pH value to 10～11, adds chloroform 20ml jolting again and extracts, divide and get chloroform layer, evaporate to dryness, residue add 1% hydrochloric acid solution 5ml makes dissolving, filters.Do two parts filtrate branch: drip bismuth potassium iodide test solution in the portion, generate salmon precipitation; Drip the silico-tungstic acid test solution in another part, generate milky white precipitate.
[inspection] should meet " relevant every regulation under granule of Chinese pharmacopoeia version in 2005 (appendix I C) item.
[assay] baicalin is according to " high effective liquid chromatography for measuring of Chinese pharmacopoeia version in 2005 (appendix VI D).
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With methanol-water-phosphoric acid (47: 53: 0.2) is mobile phase; The detection wavelength is 280nm.Number of theoretical plate should be not less than 2500 by the baicalin peak.
The preparation precision of reference substance solution takes by weighing at 4 hours baicalin reference substance of 60 ℃ of drying under reduced pressure an amount of, adds methanol and makes the solution that every 1ml contains 60ug, promptly.
This product under the content uniformity item is got in the preparation of need testing solution, and porphyrize is got about 0.5g, the accurate title, decide, and puts in the 50ml measuring bottle, adds 50% methanol, supersound process (power 250W, operating frequency 33KHZ) 20 minutes is put cold, be diluted to scale with 50% methanol, shake up, filter, precision is measured subsequent filtrate 5ml and is put in the 10ml measuring bottle, be diluted to scale with methanol, shake up, promptly.
Accurate respectively reference substance solution and each 10ul of need testing solution of drawing of algoscopy injects chromatograph of liquid, measures, promptly.
The every gram of this product contains Radix Scutellariae with baicalin (C
21H
18O
11) meter, must not be less than 9mg.
3. the phthisical medicinal granule quality standard detecting method of a kind of treatment according to claim 2, it is characterized in that at prescription constant, the extraction process condition can guarantee again under the constant situation of the phthisical base substance of its Drug therapy, quality standard detecting method is used for the check of other dosage form, same being suitable for.
4. the phthisical medicinal granule quality standard detecting method of a kind of treatment according to claim 2, it is characterized in that being used for tablet, it is the sheet of shallow palm fibre to dark brown red that the character item changes this product into, and the inspection item changes into and should meet " relevant every regulation under Chinese pharmacopoeia tablet of version in 2005 (appendix ID) item.Other quality standard detecting method is constant.
5. the phthisical medicinal granule quality standard detecting method of a kind of treatment according to claim 2, it is characterized in that being used for capsule, it is granule and the powder of shallow palm fibre to dark brown red that character changes this product content into, check that item changes and should meet that " capsule of Chinese pharmacopoeia version in 2005 (appendix I G) is relevant every regulation down, and other quality standard detecting method changes into to be got this product content and get final product into.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426955A (en) * | 2018-03-11 | 2018-08-21 | 安徽省食品药品检验研究院 | The discrimination method of tuber of stemona genunie medicinal materials |
| CN111595966A (en) * | 2020-05-18 | 2020-08-28 | 苏州必宜生物科技有限公司 | Method for measuring ethambutol in blood plasma by sensitive liquid chromatography-tandem mass spectrometry |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1602933A (en) * | 2004-08-06 | 2005-04-06 | 黄亚平 | Capsule for treating tuberculosis and its preparing process |
| CN100425276C (en) * | 2005-08-10 | 2008-10-15 | 上海中医药大学附属龙华医院 | Medicine for treating tuberculosis, and its prepn. method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426955A (en) * | 2018-03-11 | 2018-08-21 | 安徽省食品药品检验研究院 | The discrimination method of tuber of stemona genunie medicinal materials |
| CN111595966A (en) * | 2020-05-18 | 2020-08-28 | 苏州必宜生物科技有限公司 | Method for measuring ethambutol in blood plasma by sensitive liquid chromatography-tandem mass spectrometry |
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