CN101148294A - Method for increasing sulfate reduction bacterium activity - Google Patents
Method for increasing sulfate reduction bacterium activity Download PDFInfo
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- CN101148294A CN101148294A CNA2007100727660A CN200710072766A CN101148294A CN 101148294 A CN101148294 A CN 101148294A CN A2007100727660 A CNA2007100727660 A CN A2007100727660A CN 200710072766 A CN200710072766 A CN 200710072766A CN 101148294 A CN101148294 A CN 101148294A
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- reducing bacteria
- tourmalinite
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- sulphate reducing
- ferrous salt
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Abstract
The method of raising the activity of sulfate reducing bacteria is to add tourmaline and ferrous salt into water treated with the sulfate reducing bacteria. The method makes sulfate reducing bacteria grow in great amount, and can raise the activity of sulfate reducing bacteria and raise their sewage treating effect. The method can raise the activity of sulfate reducing bacteria by 8-20 %.
Description
Technical field
The present invention relates to a kind of method that improves reducing bacteria activity.
Background technology
In recent years along with the accelerated development of national economy, the trade effluent particularly pollution of sulfate wastewater is quite serious, and a lot of big cities underground water of China has been subjected to sulfate contamination in various degree.
Sulfur acid salt acid waste water directly enters water body without processing will make the receiving water body acidifying, and water pH value reduces, the harm hydrobiont, and produce potential corrosion, and destroy Soil structure, the minimizing crop yield.The sulfate wastewater latency period is long, though do not have tangible negative effect in the following short period of time of natural diluting effect, in case form pollution in wide area, then is difficult to administer and remove.
Adopt advantages such as microbiological treatment waste water is with low cost because of having, non-secondary pollution to be widely used.(sulfate reducing bacteria SRB) is the general name that a class can be reduced to sulfate radical the bacterium of sulfonium ion to sulphate reducing bacteria.Sulphate reducing bacteria extensively is present in the bed mud in paddy field, lake, natural pond and rivers and creeks and occurring in nature such as oil deposit, sulphate reducing bacteria is made of the microorganism of numerous species, comprising Gram-negative bacteria (as Desulfovibrio Desulfovibrio, Desulfobacter Desulfobacterium), gram-positive microorganism (Desulfotomaculum Desulfotomaculum), thermophilic bacterium (thermally desulfurizing Pseudomonas Thermodesulfobacterium) and thermophilic archeobacteria (ancient green-ball Pseudomonas Archaeoglobus) etc.Will utilize vitriol in the metabolism of sulphate reducing bacteria, so SRB the concentration of sulfate radical in the solution can be reduced, thereby the purpose of handling sulfate wastewater can be reached.Sulphate reducing bacteria and methanogen struggle for existence in sulfate-containing organic wastewater, the meta-bolites of sulphate reducing bacteria is a sulfide, and sulfide has toxic action to methanogen, therefore can suppress the activity of methanogen; Organic nutrient substance in the sulfate-containing organic wastewater forms hydrocarbonate (alkalescence) through the sulphate reducing bacteria oxidation, need consume oxonium ion during the sulphate reducing bacteria sulphate reducing in addition, make the pH value of water body raise, metal ion is removed with the hydroxide form precipitation, and water quality improves.Utilizing sulphate reducing bacteria (SRB) processing sulfate wastewater to obtain scientific circles at present generally approves, separation and screening, ecosystem characterization, toxicologic study, treatment process and sewage reactor etc. to SRB have carried out comparatively deep research, and the use of sulphate reducing bacteria (SRB) develops into composite S RB functional flora and SRB sludge system from original single sulphate reducing bacteria, and treatment process also develops into anaerobic mud bed, fluidized-bed process and immobilization technology by original batch precipitation; But the lower problem of sulphate reducing bacteria biological activity has restricted its development at present.
Summary of the invention
The objective of the invention is in order to solve the low problem of present sulphate reducing bacteria biological activity, and a kind of method that improves sulfate reducing bacteria activity that provides.
The method that the present invention improves sulfate reducing bacteria activity is to add tourmalinite and ferrous salt in the handled water body of sulphate reducing bacteria.
Before the present invention mainly be with nutritive element as microbial growth promotor (as anhydrous magnesium sulfate, carotene, SHUANGQITANG etc.), and the present invention is with tourmalinite and the ferrous salt growth stimulant as sulphate reducing bacteria.Adding tourmalinite and ferrous salt make dominant microflora (sulphate reducing bacteria) raised growth in the water body in the handled water body of sulphate reducing bacteria, have strengthened the activity of sulphate reducing bacteria, have improved sewage treatment effect.
Tourmalinite is big in China's standing stock, aboundresources, cheap, and its environmental activity and natural ferrimanganic aluminum oxide, silicate hydroxide minerals, zeolite, diatomite, lightweight opal are close, can not cause secondary pollution.The tourmalinite crystalline structure belongs to trigonal system, C
5 3v-R3m group so tourmalinite is the heteropolarity mineral, has spontaneous permanent electric polarity, can produce negative ion, has the adjusting water pH value, improves the sulphate reducing bacteria growing environment, improves the effect of sulfate reducing bacteria activity.
Ferrous salt is the essential nutritive element of sulfate reduction bacteria growing, is the necessary metallic element of sulfate reduction zymoprotein synthetic simultaneously, ferrous salt have an activity that helps improve sulphate reducing bacteria.Ferrous salt is used in combination with tourmalinite, and the activity of sulphate reducing bacteria improves 8%～20% under the synergy of ferrous salt and tourmalinite.
Description of drawings
Fig. 1 is that sulphate reducing bacteria does not add tourmalinite and the preceding atomic force microscope observation orthographic plan of ferrous salt in the embodiment 17; Fig. 2 is that sulphate reducing bacteria does not add tourmalinite and the preceding atomic force microscope observation solid shape figure of ferrous salt in the embodiment 17; Fig. 3 is that sulphate reducing bacteria adds atomic force microscope observation orthographic plan behind tourmalinite and the ferrous salt in the embodiment 17; Fig. 4 is that sulphate reducing bacteria adds atomic force microscope observation solid shape figure behind tourmalinite and the ferrous salt in the embodiment 17; Fig. 5 is the sulfate reducing bacteria activity correlation curve figure that adds tourmalinite and ferrous salt front and back, " ▲ " curve is for adding the preceding sulfate reducing bacteria activity curve of tourmalinite and ferrous salt among Fig. 5, and " " curve is a sulfate reducing bacteria activity curve behind adding tourmalinite and the ferrous salt.
Embodiment
Embodiment one: the method that present embodiment improves sulfate reducing bacteria activity is to add tourmalinite and ferrous salt in the handled water body of sulphate reducing bacteria.
Sulphate reducing bacteria in the present embodiment can be single sulphate reducing bacteria, composite S RB functional flora or SRB sludge system.The trace element that tourmalinite contains can promote the growth of sulphate reducing bacteria.
Embodiment two: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added ferrous salt 0.05～2g in the water body.Other is identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added ferrous salt 0.1～1.8g in the water body.Other is identical with embodiment one.
Embodiment four: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added ferrous salt 0.2～1.5g in the water body.Other is identical with embodiment one.
Embodiment five: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added ferrous salt 0.5～1.3g in the water body.Other is identical with embodiment one.
Embodiment six: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added ferrous salt 0.8～1.1g in the water body.Other is identical with embodiment one.
Embodiment seven: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added ferrous salt 1g in the water body.Other is identical with embodiment one.
Embodiment eight: present embodiment and embodiment one, two, three, four, five, six or sevens' difference is: ferrous salt is ferrous ammonium sulphate, ferrous ammonium phosphate or iron protochloride ammonium.Other is identical with embodiment one, two, three, four, five, six or seven.
Embodiment nine: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added tourmalinite 0.05～0.2g in the water body.Other is identical with embodiment one.
Embodiment ten: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added tourmalinite 0.08～0.18g in the water body.Other is identical with embodiment one.
Embodiment 11: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added tourmalinite 0.1～0.16g in the water body.Other is identical with embodiment one.
Embodiment 12: the difference of present embodiment and embodiment one is: every 100mL sulphate reducing bacteria is handled and is added tourmalinite 0.12～0.14g in the water body.Other is identical with embodiment one.
Embodiment 13: present embodiment and embodiment one, nine, ten, 11 or 12 difference are: the particle diameter of tourmalinite is 50nm～1cm.Other is identical with embodiment one, nine, ten, 11 or 12.
Embodiment 14: present embodiment and embodiment one, nine, ten, 11 or 12 difference are: the particle diameter of tourmalinite is 50nm～100nm.Other is identical with embodiment one, nine, ten, 11 or 12.
Embodiment 15: present embodiment and embodiment one, nine, ten, 11 or 12 difference are: the particle diameter of tourmalinite is 60nm～90nm.Other is identical with embodiment one, nine, ten, 11 or 12.
Embodiment 16: present embodiment and embodiment one, nine, ten, 11 or 12 difference are: the particle diameter of tourmalinite is 70nm～80nm.Other is identical with embodiment one, nine, ten, 11 or 12.
Embodiment 17: the method that present embodiment improves sulfate reducing bacteria activity is to add tourmalinite and ferrous salt in the handled water body of sulphate reducing bacteria; Every 100mL sulphate reducing bacteria is handled and is added iron protochloride ammonium 2g in the water body; Every 100mL sulphate reducing bacteria handles in the water body that to add particle diameter be the tourmalinite 0.1g of 50nm～100nm.
The sulphate reducing bacteria of present embodiment is single sulphur Vibrio bacterium.Fig. 1,2,3 and 4 is the atomic force microscope observation figure of sulphate reducing bacteria, there is notable difference in contrast by Fig. 1 and Fig. 3, Fig. 2 and Fig. 4 sulphate reducing bacteria as can be seen adding tourmalinite and ferrous salt front and rear surfaces form, sulphate reducing bacteria thalline surface irregularity behind adding tourmalinite and the ferrous salt has adsorbed a large amount of tourmalinite.
Adopt the sulfate reduction activity determination method to measure the activity (reductase enzyme enzyme activity) of adding tourmalinite and ferrous salt front and back sulphate reducing bacteria, the enzyme biopsy is surveyed the result as shown in Figure 5; Detect and illustrate that present embodiment adds tourmalinite in the handled water body of sulphate reducing bacteria and ferrous salt can improve sulfate reducing bacteria activity 10%～15%.
Claims (7)
1. method that improves sulfate reducing bacteria activity, the method that it is characterized in that improving sulfate reducing bacteria activity is to add tourmalinite and ferrous salt in the handled water body of sulphate reducing bacteria.
2. a kind of method that improves sulfate reducing bacteria activity according to claim 1 is characterized in that every 100mL sulphate reducing bacteria is handled in the water body and adds ferrous salt 0.05～2g.
3. a kind of method that improves sulfate reducing bacteria activity according to claim 1 and 2 is characterized in that ferrous salt is ferrous ammonium sulphate, ferrous ammonium phosphate or iron protochloride ammonium.
4. a kind of method that improves sulfate reducing bacteria activity according to claim 1 is characterized in that every 100mL sulphate reducing bacteria is handled in the water body and adds tourmalinite 0.05～0.2g.
5. according to claim 1 or 4 described a kind of methods that improve sulfate reducing bacteria activity, the particle diameter that it is characterized in that tourmalinite is 50nm～1cm.
6. according to claim 1 or 4 described a kind of methods that improve sulfate reducing bacteria activity, the particle diameter that it is characterized in that tourmalinite is 50nm～100nm.
7. according to claim 1 or 4 described a kind of methods that improve sulfate reducing bacteria activity, the particle diameter that it is characterized in that tourmalinite is 60nm～90nm.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100727660A CN100558657C (en) | 2007-09-06 | 2007-09-06 | A kind of method that improves sulfate reducing bacteria activity |
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| CNB2007100727660A CN100558657C (en) | 2007-09-06 | 2007-09-06 | A kind of method that improves sulfate reducing bacteria activity |
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| CN101148294A true CN101148294A (en) | 2008-03-26 |
| CN100558657C CN100558657C (en) | 2009-11-11 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973610A (en) * | 2010-04-22 | 2011-02-16 | 吉林大学 | Method for in-situ remediation of petroleum hydrocarbon polluted underground water by taking tourmaline as additive medium |
| CN103739065A (en) * | 2013-12-04 | 2014-04-23 | 吉林省电力科学研究院有限公司 | Anaerobic biological treatment method of heat-engine plant desulphurization waste water |
| CN105621625A (en) * | 2014-10-28 | 2016-06-01 | 中国石油化工股份有限公司 | Nitrite bacteria growth promoter and preparation method thereof |
| CN106867880A (en) * | 2017-02-15 | 2017-06-20 | 山东科技大学 | A kind of sulfate reducing bacteria induces the disposable experimental provision and its experimental technique of carbonate mineral |
| CN111717965A (en) * | 2019-03-20 | 2020-09-29 | 中国石油化工股份有限公司 | Electro-filtration sterilization method for sulfate reducing bacteria |
| CN111925063A (en) * | 2020-08-14 | 2020-11-13 | 中国地质科学院水文地质环境地质研究所 | Simulated remediation system and method for 1,2-dichloroethane and sulfate-containing groundwater |
-
2007
- 2007-09-06 CN CNB2007100727660A patent/CN100558657C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973610A (en) * | 2010-04-22 | 2011-02-16 | 吉林大学 | Method for in-situ remediation of petroleum hydrocarbon polluted underground water by taking tourmaline as additive medium |
| CN101973610B (en) * | 2010-04-22 | 2013-08-07 | 吉林大学 | Method for in-situ remediation of petroleum hydrocarbon polluted underground water by taking tourmaline as additive medium |
| CN103739065A (en) * | 2013-12-04 | 2014-04-23 | 吉林省电力科学研究院有限公司 | Anaerobic biological treatment method of heat-engine plant desulphurization waste water |
| CN105621625A (en) * | 2014-10-28 | 2016-06-01 | 中国石油化工股份有限公司 | Nitrite bacteria growth promoter and preparation method thereof |
| CN105621625B (en) * | 2014-10-28 | 2018-03-16 | 中国石油化工股份有限公司 | A kind of nitrite bacteria growth promoter and preparation method thereof |
| CN106867880A (en) * | 2017-02-15 | 2017-06-20 | 山东科技大学 | A kind of sulfate reducing bacteria induces the disposable experimental provision and its experimental technique of carbonate mineral |
| CN106867880B (en) * | 2017-02-15 | 2018-09-07 | 山东科技大学 | A kind of disposable experimental provision and its experimental method of sulfate reducing bacteria induction carbonate mineral |
| CN111717965A (en) * | 2019-03-20 | 2020-09-29 | 中国石油化工股份有限公司 | Electro-filtration sterilization method for sulfate reducing bacteria |
| CN111717965B (en) * | 2019-03-20 | 2022-06-28 | 中国石油化工股份有限公司 | Electro-filtration sterilization method for sulfate reducing bacteria |
| CN111925063A (en) * | 2020-08-14 | 2020-11-13 | 中国地质科学院水文地质环境地质研究所 | Simulated remediation system and method for 1,2-dichloroethane and sulfate-containing groundwater |
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| CN100558657C (en) | 2009-11-11 |
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Granted publication date: 20091111 Termination date: 20100906 |