CN101143157A

CN101143157A - Tyrosol and tyrosol bypass salidroside plant extraction and preparation and use thereof

Info

Publication number: CN101143157A
Application number: CNA2006100960548A
Authority: CN
Inventors: 肖伟; 蔡宝昌; 李明慧; 丁岗; 朱华荣; 王成生; 曹亮
Original assignee: Jiangsu Zeukov Pharmaceutical S & T Inc
Current assignee: Jiangsu Zeukov Pharmaceutical S & T Inc
Priority date: 2006-09-11
Filing date: 2006-09-11
Publication date: 2008-03-19

Abstract

The invention discloses an extract of a tyrosol plant, which is extracted from a root or a rhizome of a rhodiola rosea or from a mature fruit or a flower of a glossy privet fruit or from fermented soy sauce or vinegar residue. The invention also discloses an extract preparation of the tyrosol plant and the purpose of the extract preparation. The invention also discloses an extract of a salidroside plant of the tyrosol side product and the preparation and purpose of the extract. The extraction technology of the extracts of the invention is advanced, and the content of the effective components of the extracts is high, and the extracts can be fit for the requirements of the industrialized production technology and the pharmacy.

Description

Butyl alcohol and butyl alcohol by-product salidroside plant extraction and preparation and purposes

Technical field

The present invention relates to a kind of Chinese medicine extract, particularly a kind of butyl alcohol plant extract and preparation and purposes the invention still further relates to a kind of butyl alcohol by-product salidroside plant extraction and preparation and purposes.

Background technology

Butyl alcohol is at root or the rhizome of Crassulaceae rhodiola (RhodiolaL.) plant, perhaps the mature fruit of Oleaceae aiphyllium Fructus Ligustri Lucidi Ligustrum lucidum A it. or spend in discovery is all arranged, be the aglycon of rhodioside.Below be the structural formula of rhodioside:

The molecular formula of butyl alcohol (Tryosol): C ₈H ₁₀O ₂Structural formula:

Document is reported for work, and Radix Rhodiolae extract, Fructus Ligustri Lucidi extract have multiple efficacies such as immunoregulation effect, antivirus action, oxygen lack resistant function, hypoglycemic activity, neurocyte protection effect, liver protection, nootropic effect, antioxidation, radiation protection.Wherein main effective ingredient is butyl alcohol and rhodioside.Since Radix Rhodiolae extract, Fructus Ligustri Lucidi extract component complexity, so extract dna purity of the prior art is lower, and the present extraction process technology of also finding the specification requirement of adaptation suitability for industrialized production, meeting the butyl alcohol and the rhodioside of pharmacy requirement.

Summary of the invention

Technical problem to be solved by this invention is at the deficiencies in the prior art, and a kind of extraction process advanced person, butyl alcohol plant extract that effective component content is high are provided.

Another technical problem to be solved by this invention provides a kind of butyl alcohol plant extract preparation.

Another technical problem to be solved by this invention provides the purposes of above-mentioned butyl alcohol plant extract and preparation thereof.

Another technical problem to be solved by this invention provides a kind of butyl alcohol by-product salidroside plant extraction.

Another technical problem to be solved by this invention provides a kind of butyl alcohol by-product salidroside plant extraction preparation.

Another technical problem to be solved by this invention provides the purposes of above-mentioned butyl alcohol by-product salidroside plant extraction and preparation thereof.

Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is a kind of butyl alcohol plant extract, is characterized in, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) get raw material, extract or the percolation extraction with alcohol reflux or dipping, collect extracting solution, being concentrated into does not have the alcohol flavor, adds the water-cooled Tibetan, goes precipitation, and aqueous solution is regulated PH to 2～4 with acid solution, and cold preservation is gone precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with deionized water eluting macroporous resin column earlier to effluent, wash nearly neutrality again with alkaline solution eluting macroporous resin column, and then with deionization, with the acid solution eluting, be eluted to partial neutral with deionized water more again, above eluent all discards; With 5%～30% ethanol elution, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, and clear liquor concentrates, and adds the precipitation organic solvent, fully stirs, and precipitation is gone in cold preservation, get clear liquor concentrated after, the adding chromatographic silica gel is mixed sample, evaporate to dryness, last silica gel column chromatography;

(4) organic solvent or mixed organic solvents carry out remove impurity and eluting or gradient elution, the employing thin layer chromatography detects, collecting principal spot is the eluent of butyl alcohol, reclaim eluent, get solid matter and carry out recrystallization with organic solvent with recrystallization, obtain the white plates crystallization, be the butyl alcohol plant extract.

Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of butyl alcohol plant extract is characterized in, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) gets raw material, extract 2～4 times with 50%～90% alcohol reflux or dipping, add 5～15 times of amounts at every turn, perhaps with 50%～90% ethanol percolate extraction of 15～30 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 1～10 times of water gaging cold preservation is spent the night or cold preservation 6～24 hours, goes precipitation, aqueous solution is regulated PH2～4 with acid solution, cold preservation is spent the night or cold preservation 6～24 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with 1～4 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 2～5 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 1～4 times of column volume, again with 1～4 times of column volume acid solution eluting, be eluted to partial neutral with 1～4 times of column volume neutral water again, above eluent all discards; With 10～20 times of column volume 5%～30% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 2～5g raw material/ml, adds 5～20 times of precipitation organic solvents, fully stirs, cold preservation 6～24 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 40～70, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio (bore and height) is 1: 9～15;

Technical problem to be solved by this invention can also further realize by following technical scheme.The invention also discloses a kind of butyl alcohol by-product salidroside plant extraction, be characterized in, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(4) organic solvent or mixed organic solvents carry out remove impurity and eluting or gradient elution, the employing thin layer chromatography detects, collecting principal spot is the eluent of butyl alcohol, reclaim eluent, get solid matter and carry out recrystallization with organic solvent with recrystallization, obtain the white plates crystallization, be major product butyl alcohol plant extract; After the collection principal spot is the eluent of butyl alcohol, select another kind of mixed organic solvents, continue gradient elution, the employing thin layer chromatography detects, and collecting principal spot is the eluent of rhodioside, concentrates, leave standstill, get white crystalline material, be the side-product salidroside plant extraction.

Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of butyl alcohol by-product salidroside plant extraction is characterized in, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 2～5g raw material/ml, adds 5～20 times of precipitation organic solvents, fully stirs, cold preservation 6～24 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 40～70, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 9～15;

In the technical scheme of above-mentioned any one butyl alcohol plant extract or butyl alcohol by-product salidroside plant extraction, step (1) and the described acid solution of step (2) are hydrochloric acid solution, sulfuric acid solution, acetum or the phosphoric acid solution of 0.01～1mol/L; The described alkaline solution of step (2) is that 0.001%～1% sodium hydroxide solution, aqua calcis, barium hydroxide, ammonia spirit, ethylenediamine solution, sodium carbonate liquor, solution of potassium carbonate, sodium bicarbonate solution, potassium bicarbonate solution, potassium hydroxide solution or other are not the aqueous solution of the alkaline matter of resin absorption; The described dissolving of step (3) refers to 90% above concentration ethanol, dehydrated alcohol, methanol, acetone, n-butyl alcohol or ethyl acetate with organic solvent; The described precipitation organic solvent of step (3) is acetone, ethyl acetate, petroleum ether, n-hexane, n-butyl alcohol, dichloromethane, chloroform, ether or other weakly polar organic solvent; The described organic solvent of step (4) is n-butyl alcohol, chloroform, dichloromethane or petroleum ether; 7～3) or petroleum ether 7～3), petroleum ether 7～3), dichloromethane 7～3), chloroform 7～3), n-butyl alcohol 3～1), ethyl acetate described mixed organic solvents is an ethyl acetate: acetone (7～9:: n-hexane (3～7:: n-hexane (3～7:: n-hexane (3～7:: n-hexane (3～7:: n-hexane (3～7:: ethyl acetate (3～7: 7～3); The described recrystallization organic solvent of step (4) is ethyl acetate, petroleum ether, n-hexane, dichloromethane or chloroform.

Technical problem to be solved by this invention can also further realize by following technical scheme.The invention also discloses a kind of butyl alcohol extract formulation, be characterized in, it be the butyl alcohol plant extract and pharmaceutically the acceptable pharmaceutical carrier make the medicament of acceptable any dosage form on the pharmaceutics.

Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of butyl alcohol extract formulation is characterized in, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 0.1%-99.5%, preferred 0.5%-95%.

Technical problem to be solved by this invention can also further realize by following technical scheme.The invention also discloses a kind of butyl alcohol by-product salidroside plant extraction preparation, be characterized in, it be butyl alcohol plant extract and salidroside plant extraction and pharmaceutically the acceptable pharmaceutical carrier make the medicament of acceptable any dosage form on the pharmaceutics.

Technical problem to be solved by this invention can also further realize by following technical scheme.Above-described a kind of butyl alcohol by-product salidroside plant extraction preparation is characterized in that wherein, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 0.1～10.

A kind of butyl alcohol plant extract of the present invention and preparation thereof and a kind of butyl alcohol by-product salidroside plant extraction and preparation thereof can be used for preparing the medicine for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps are used for preparing the medicine of treatment coronary heart disease, cerebral infarction, viral myocarditis and alzheimer disease.

In the gained butyl alcohol plant extract of the present invention, butyl alcohol content can reach more than 90.0%; The content of rhodioside can reach more than 90.0% in the salidroside plant extraction; In the butyl alcohol by-product salidroside plant extraction, the content sum of rhodioside and butyl alcohol can reach more than 90.0%;

The specific embodiment

Embodiment 1.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) 60% ethanol is carried 2 times, each 10 times of amounts, and being concentrated into does not have the alcohol flavor, adds 3 times of water gaging cold preservations and spends the night, the centrifugal precipitation of going, clear liquor is regulated PH=4 with 0.1mol/L hydrochloric acid, and cold preservation is spent the night, the centrifugal precipitation of going, AB-8 macroporous resin on the clear liquor, resin demand are raw-material 3 times;

(2) closely neutral with 2 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 5 times of column volumes, 0.1% sodium hydroxide eluting macroporous resin column, and then wash nearly neutrality with 2 times of column volumes, again with 2 times of column volume 0.1mol/L hydrochloric acid eluting, 2 times of column volume neutral waters are eluted to partial neutral, and above eluent all discards; With 15 times of column volume 5% ethanol elutions, collect eluent again;

(3) concentrate drying back dissolve with methanol, centrifugal removal insoluble matter, clear liquor is concentrated into 3g crude drug/ml, adds 10 times of petroleum ether, fully stirs, cold preservation is spent the night, the centrifugal precipitation of going keeps clear liquor, concentrating clarifying liquid, with silica gel mixed sample (1 gram silica gel/55 gram raw materials), water bath method, last silicagel column (1 gram crude drug/1.6 gram silica gel, blade diameter length ratio is 1: 10);

(4) ethyl acetate-acetone (8: 2) eluting, TLC follows the tracks of detection, and collecting principal spot is the eluent of butyl alcohol, is that solvent carries out recrystallization with the dichloromethane, obtains white plates crystallization butyl alcohol plant extract.

Embodiment 2.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(1) 75% alcohol extraction 3 times (being respectively 10 times, 8 times, 8 times), being concentrated into does not have the alcohol flavor, adds 8 times of water gaging cold preservations and spends the night, the centrifugal precipitation of going, aqueous solution is regulated PH=3 with 0.5mol/L hydrochloric acid, and cold preservation is spent the night, the centrifugal precipitation of going, D101 macroporous resin on the clear liquor, resin demand are raw-material 2.5 times;

(2) closely neutral with 3 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 3 times of column volumes, 0.5% sodium hydroxide eluting macroporous resin column, and then wash nearly neutrality with 3 times of column volumes, again with 3 times of column volume 0.5mol/L hydrochloric acid eluting, 2 times of column volume neutral waters are eluted to partial neutral

Above eluent all discards; With 15 times of column volume 10% ethanol elutions, collect eluent again;

(3) concentrate drying back dissolve with methanol, centrifugal removal insoluble matter, clear liquor is concentrated into 5g crude drug/ml, adds 12 times of chloroforms, fully stirs, cold preservation is spent the night, the centrifugal precipitation of going keeps clear liquor, concentrating clarifying liquid, with silica gel mixed sample (1 gram silica gel/60 gram raw materials), water bath method, last silicagel column (1 gram crude drug/1 gram silica gel, blade diameter length ratio is 1: 10);

(4) 1.5 times of column volumes of ethyl acetate-chloroform (3: 7) eluting, eluent is put in addition, then with ethyl acetate-n-hexane (4: 6), eluting, collecting principal spot is the eluent of butyl alcohol, is that solvent carries out recrystallization with the dichloromethane, obtain the white plates crystallization, be the butyl alcohol plant extract; Adopt 1 times of column volume of ethyl acetate-acetone (7: 3) eluting then, again with ethyl acetate-acetone (4: 6) eluting, collecting principal spot is the eluent of rhodioside, concentrates, and leaves standstill, and gets white crystalline material, is salidroside plant extraction.

Embodiment 3.The pharmacological action experiment of the butyl alcohol plant extract (hereinafter to be referred as butyl alcohol) that makes by embodiment 1.

Experiment purpose:, determine the pharmacological action that butyl alcohol is basic by pharmacological evaluation.

One, experiment material

(1) medicine and reagent

Butyl alcohol is provided by Jiangsu Zhongkang Medicine Science ﹠ Technology Co., Ltd; Radix Salviae Miltiorrhizae Tabellae, 100 slices/bottle, Shanghai Leiyun Pharmaceutical Industry Co., Ltd., lot number: 0404031; SHENGMAI JIAONANG, the about 12g of people's consumption (crude drug)/kg, Jiangxi Huiren Pharmaceutical Co., Ltd, lot number: 0412026; Sodica calx, the land, Shanghai is chemical reagent factory all, lot number: 20030612; Chloral hydrate: AR, 250g/ bottle, Chemical Reagent Co., Ltd., Sinopharm Group, lot number: 20040420; Normal saline, Nanjing Xiaoying Pharmaceutical Factory, lot number: 20050525; Fluorouracil Injection, 0.25g/10ml, Tianjin gold credit aminoacid company limited, lot number: 0505231.

(2) animal

Cleaning level SD rat, ICR mice are provided production licence: SCXK (Soviet Union) 2002-0031 by Nanjing Medical University's Experimental Animal Center; Jiangsu Zhongkang Medicine Science ﹠ Technology Co., Ltd, occupancy permit: SYXK (Soviet Union) 2003-0029.

Two, experiment condition and statistical method

(1) experiment condition

Before and after the administration, the laboratory animal male and female are divided cage, feed with laboratory animal mixed feed (being provided by Nanjing An Limo Science and Technology Ltd.), freely drink water 20～24 ℃ of room temperatures, humidity 40～70%.

(2) statistical method

Data represents that with mean ± standard deviation (x ± s), the quantitative response data adopts Microsoft Excel software to carry out Student ' s-t check, and the qualitative response data adopts X 2 test.

Three, experimental technique and result

(1) to the influence of mice normal pressure hypoxia-bearing capability

1. grouping and administration

Get 100 of ICR mices, be divided into 5 groups at random, 20 every group.Be model group, SHENGMAI JIAONANG group (4g crude drug/kg), butyl alcohol be little, in, heavy dose of group (1.7,5.1,15.3mg/kg), the administration volume is 20ml/kg, model group is irritated stomach and is given the isometric(al) distilled water, successive administration 5 days.

2. observation index and method

After the 5th day mice last irritated stomach 0.5h.Mice is placed the airtight wide mouthed bottle of the 250ml that sodica calx 10g is housed respectively, and bottle cap is coated with the vaseline sealing, observes mice and ceases breathing the time (time-to-live), compares with the normal control group.

3. experimental result

The model group mice is in the time of 14 minutes, and most of all dead, its time-to-live is 816 seconds.Each administration group all can increase the time-to-live of mice in various degree, has statistical significance (P＜0.05, P＜0.01).The results are shown in Table 1.

The influence of table 1 pair mice normal pressure anoxia enduring time-to-live (x ± s, n=20)

Grouping	Dosage (mg/kg)	Time-to-live (second)
Grouping	Dosage (mg/kg)	Time-to-live (second)	Model group SHENGMAI JIAONANG group	— 4×10 ³	816±229 1063±267 ^**

The heavy dose of group of dosage group butyl alcohol in the butyl alcohol small dose group butyl alcohol

1.7 5.1 15.3

994±184 ^* 1134±249 ^** 1249±194 ^**

Annotate: compare with model group, ^*P＜0.05, ^*P＜0.01.

(2) to the influence of rat coronary ligation

1. grouping and administration

60 of SD rats are divided into 6 groups at random, 10 every group.Be normal control group, model group, Radix Salviae Miltiorrhizae Tabellae group (lg crude drug/kg), butyl alcohol be little, in, heavy dose of group (0.85,2.55,7.65mg/kg), the administration volume is 10ml/kg, normal control group and model group are irritated stomach and are given the isometric(al) distilled water, successive administration 5 days.

2. observation index and method

The rat chloral hydrate (lie on the back and be fixed in operating-table, and tracheal intubation is connected to respirator, practices artificial respiration by 350mg crude drug/kg) anesthesia.Chest unhairing, sterilization, along the about 2cm of left mid-clavicular line longitudinal incision skin, 4 ~ 5 intercostal passivity separating muscles are opened the thoracic cavity from the left side, cut off pericardium, gently press the right side thorax, extrude heart.Between arterial cone and the left auricle with 5/0 stitching thread ligation left coronary artery (LAD) after, send heart back to thoracic cavity rapidly, sew up thoracic wall immediately.Stop the artificial respiration. ^[1]Behind the ligation LAD 24 hours, take off cervical vertebra and put to death rat, open breast simultaneously and core dirtyly, remove atrial tissue, satisfactory chamber weight in wet base along coronary sulcus again.Parallel coronary sulcus is 5 ~ 6 of ventricle crosscuts, is positioned in 37 ℃ the 0.5%TTC phosphate buffer to dye.Separate infarcted region (not tinter) and its weight in wet base of weighing in the cardiac muscle, calculate the percentage ratio that infarcted myocardium accounts for ventricle.

3. experimental result

The no infarction of rats in normal control group cardiac muscle, tangible infarction appears in the model group rat.Each administration group rat heart muscle infarction size is all little than model group, wherein in Radix Salviae Miltiorrhizae Tabellae group, the butyl alcohol, heavy dose of group compares with model group, and significant difference (P＜0.05, P＜0.01) is arranged.The results are shown in Table 2.

The influence of table 2 pair rat coronary ligation myocardial infarct size (x ± s, n=10)

Grouping	Dosage (mg/kg)	Ventricle heavy (g)	Infarcted region heavy (g)	Infarcted region/ventricle (%)
Grouping	Dosage (mg/kg)	Ventricle heavy (g)	Infarcted region heavy (g)	Infarcted region/ventricle (%)	End the heavy dose of group of dosage group butyl alcohol in the normal matched group model group Radix Salviae Miltiorrhizae Tabellae group butyl alcohol small dose group butyl alcohol	— — 1.0×10 ³ 0.85 2.55 7.65	76.8±9.7 81.7±12.8 79.2±12.4 76.2±11.2 81.3±13.5 77.4±12.4	0.0±0.0 23.7±9.6 13.8±7.9 ^* 17.2±7.3 12.7±9.1 ^* 10.8±7.2 ^**	0.0±0.0 28.7±11.9 18.6±8.6 ^* 22.3±10.2 17.3±8.5 ^* 13.8±9.3 ^**

Annotate: compare with model group, ^*P＜0.05, ^*P＜0.01.

(3) virus causes the protective effect of mouse cardiac muscle damage

1. grouping and administration

6～8 ages in week, male BALB-C mice was 60, was divided into 6 groups at random, 10 every group.Be normal control group, model group, SHENGMAI JIAONANG group (4g crude drug/kg), butyl alcohol be little, in, heavy dose of group (1.7,5.1,15.3mg/kg), the administration volume is 20ml/kg, model group is irritated stomach and is given the isometric(al) distilled water, successive administration 12 days.

2. observation index and method

With close Mus cardiac muscle strain CVB3 purification liquid 1.0 * 107pfu/ml lumbar injection male BALB-C mice in 6～8 age in week that Woodruffs cultivates, make chmice acute myocarditis model.10 of normal control groups, injecting normal saline 0.4ml.Successive administration is also observed 12d, during this dying mice is in time put to death, and gets the hematometry serum activity of SOD, and the dirty routine pathology inspection of carrying out of coring, and respectively organizes whole the putting to death of survivor to 12d and does the same inspection.

3. experimental result

Mouse death rate obviously raises after the modeling, myocardium pathologic finding shows inflammatory cell infiltration and necrosis is obvious, activity of SOD in serum is starkly lower than normal group, and statistical significance (P＜0.01) is arranged.Each treatment group infects back mortality rate decline, cardiac muscular tissue's inflammatory cell infiltration and necrosis and all obviously is lighter than not treatment group, and each dosage group of butyl alcohol all can obviously improve activity of SOD in serum (p＜0.05, p＜0.01).The results are shown in Table 3,4,5.

Table 3 pair virus cause mouse cardiac muscle damage mortality rate influence (x ± s, n=10)

Grouping	Dosage (mg/kg)	Death toll	Mortality rate
Grouping	Dosage (mg/kg)	Death toll	Mortality rate	The heavy dose of group of dosage group butyl alcohol in the normal control group model group SHENGMAI JIAONANG group butyl alcohol small dose group butyl alcohol	— — 4.0×10 ³ 1.7 5.1 15.3	0 8 4 4 3 1	0 80 40 ^ 40 ^ 30 ^ 10 ^

Annotate: compare with model group, ^*P＜0.01.

Table 4 pair virus cause the mouse cardiac muscle histopathology influence (x ± s, n=10)

Grouping	Dosage (mg/kg)	The necrosis region number	Inflammatory infiltration kitchen range number
Grouping	Dosage (mg/kg)	The necrosis region number	Inflammatory infiltration kitchen range number	End normal matched group model group SHENGMAI JIAONANG group butyl alcohol small dose group	— — 4.0×10 ³ 1.7	0 23.5±13.7 12.6±8.1 ^* 13.5±6.6	0 20.1 ±9.43 10.1±8.6 ^* 9.1±7.8 ^*

Annotate: compare with model group, ^*P＜0.05, ^*P＜0.0l.

Table 5 pair virus cause mice serum SOD vigor influence (x ± s, n=10)

Annotate: compare with the normal control group, ^{△ △}P＜0.01; Compare with model group, ^*P＜0.05, ^*P＜0.01.

(4) to S ₁₈₀The influence of transplanted tumor tumour inhibiting rate

1. grouping and administration

50 of ICR mices are divided into 5 groups at random, 10 every group.Be model group, 5-Fu group (25mg/kg), butyl alcohol little, in, heavy dose of group (1.7,5.1,15.3mg/kg), the administration volume is 20ml/kg, model group is irritated stomach and is given the isometric(al) distilled water, successive administration 8 days.

2. observation index and method

Aseptic condition extracts 8 days ascites cells suspension of growth in the ICR mouse peritoneal down, and cancerous cell suspension and sterile saline are got 50 of ICR mices by dilution in 1: 3, and the cancerous cell suspension 0.2ml after the inoculation dilution is subcutaneous in armpit.Be divided into 5 groups behind the 24h at random, irritate stomach respectively and give relative medicine (model group is irritated stomach and given the isometric(al) distilled water), put to death mice after 8 days, strip tumor, weigh, calculate tumour inhibiting rate by following formula.Experiment repeats 2 times.

3. experimental result

Mouse inoculation S ₁₈₀After the tumor, three days subcutaneous can contact tumor, dissects exemplary embodiment lock after 8 days all greater than 1g, and three dosage groups of LK mouse tumor soaks into scope all less than model group, and the tumor body is easily peeled off, and tumor weight obviously alleviates (P＜0.05, P＜0.01).Tumour inhibiting rate is greater than 40%.The results are shown in Table 1.

Table 6 couple S ₁₈₀The influence of transplanted solid tumor tumour inhibiting rate (x ± s, n=10)

Annotate: compare with model group, ^*P＜0.05, ^*P＜0.01.

(5) to the influence of Senile Dementia Model Rats

L. grouping and administration

560 of Wister rats are divided into 6 groups at random, and every group of l0 only.Be normal control group, model group, melatonin group (5mg/kg), butyl alcohol little, in, heavy dose of group (0.85,2.55,7.65mg/kg), the administration volume is 10ml/kg, normal control group and model group are irritated stomach and are given the isometric(al) distilled water, 4 weeks of successive administration.

2. observation index and method

Intraperitoneal injection D-galactose 50mg/ (kg.d) continued for 6 weeks, injected each 1 μ L modeling of A β 1-40 (containing A β 1-40 4 μ g, with physiological saline solution), normal control group injecting normal saline the 4th weekend in the bilateral Hippocampus.Modeling begins administration after 2 weeks, altogether 4 weeks of administration.

Injecting step in the medicine Hippocampus:

Rat is fixed on the brain solid positioner after anaesthetizing with 2.5% pentobarbital sodium, (with the bregma is zero point behind the Hippocampus of three-dimensional location, AP=-315mm, ML=210mm, DV=310mm), bore and open skull, pass through microsyringe, each injecting normal saline of Hippocampus or medicine 1 μ L (containing A β 1-40 4 μ g) to the left and right with physiological saline solution.

The learning and memory performance testing:

Adopt Y2 type maze method.Experiment is defined in continuous ten tests animal to be had and correctly enters the place of safety nine times and be decided to be the standard that reaches association.Write down the number of times that every rat reaches the required training of association's standard and originally test all detections, judgement, recording process by computer control.

Hippocampal tissue SOD vigor, the MDA assay:

The hippocampal tissue homogenate of preparation 5%, centrifugal, get supernatant SOD, the MDA kit measurement.Measure protein content with the Coomassie brilliant blue method.

3. experimental result

Reach the frequency of training of association's standard in rat Y-type labyrinth, three dosage groups of butyl alcohol are starkly lower than model group (P＜0.05), and are similar with the melatonin group.Model group is compared with normal group, and SOD vigor compensatory raises, and MDA content raises, and shows that the free-radical oxidation product increases in the brain.Compare with model group, three dosage groups of butyl alcohol rat hippocampus SOD vigor, MDA content reduce, and have statistical significance (P＜0.05).The results are shown in Table 7,8.

The influence of table 7 pair learning and memory in rats behavior (x ± s, n=10)

Grouping	Dosage (mg/kg)	Frequency of training
Grouping	Dosage (mg/kg)	Frequency of training	The heavy dose of group of dosage group butyl alcohol in the normal control group model group melatonin group butyl alcohol small dose group butyl alcohol	— — 5 0.85 2.55 7.65	20.6±8.3 43.4±12.1 ^△△ 26.5±8.4 ^* 31.9±9.7 ^ 28.3±7.5 ^ 23.7±6.2 ^**

Annotate: with end normal matched group relatively, ^{△ △}P＜0.01; Compare with model group, ^*P＜0.05, ^*P＜0.01.

The influence of table 8 pair AD rat hippocampus SOD vigor and MDA content (x ± s, n=10)

Grouping	Dosage (mg/kg)	SOD(nu/mg pro)	MDA(mm/mgpro)
Grouping	Dosage (mg/kg)	SOD(nu/mg pro)	MDA(mm/mgpro)	The heavy dose of group of dosage group butyl alcohol in the normal control group model group melatonin group butyl alcohol small dose group butyl alcohol	— — 5 0.85 2.55 7.65	134.2±26.4 462.8±46.9 ^△△ 264.7±39.1 ^ 311.6±48.7 ^ 253.7±34.7 ^ 211.3±40.8 ^	263.4 ±46.2 451.9±51.7 ^△△ 361.9±42.6 ^ 381.6±51.6 ^ 360.3±42.9 ^ 316.2±43.7 ^

(4) conclusion

1, by the experiment of mice anoxia enduring, butyl alcohol can significantly increase the time-to-live of anoxia enduring mice.

2, by the experiment of rat coronary ligation, butyl alcohol can reduce rat model heart Infarction volume.

3, cause the mouse cardiac muscle injury experiment by virus, butyl alcohol can reduce viral myocardial damage mouse death rate, improves myocardial necrosis and cell infiltration, and the rising serum activity of SOD causes the mouse cardiac muscle damage to virus and has certain therapeutical effect.

4, pass through S ₁₈₀The solid tumor experiment, butyl alcohol has certain anti-S ₁₈₀The solid tumor effect.

5, by the AD rat model, butyl alcohol can improve learning and memory function, reduces rat hippocampus tissue SOD's vigor and MDA content, and the AD rat is had certain therapeutical effect.

In sum, butyl alcohol has anti-cerebral anoxia, function of resisting myocardial ischemia; Virus is caused the mouse cardiac muscle damage have significant therapeutical effect; To S ₁₈₀Transplanted solid tumor has the good antitumor effect; The AD rat had certain therapeutical effect.Disclose butyl alcohol at the preparation antitumor drug, in preparation treatment coronary heart disease, cerebral infarction, viral myocarditis and the alzheimer disease medicine, have a good application prospect.

Embodiment 4.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) get raw material, extract or the percolation extraction with alcohol reflux or dipping, collect extracting solution, being concentrated into does not have the alcohol flavor, adds the water-cooled Tibetan, goes precipitation, and aqueous solution is regulated PH to 3 with acid solution, and cold preservation is gone precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with deionized water eluting macroporous resin column earlier to effluent, wash nearly neutrality again with alkaline solution eluting macroporous resin column, and then with deionization, with the acid solution eluting, be eluted to partial neutral with deionized water more again, above eluent all discards; With 20% ethanol elution, collect eluent again;

Embodiment 5.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) gets raw material, extract 3 times with 70% alcohol reflux or dipping, add 10 times of amounts at every turn, perhaps with 70% ethanol percolate extraction of 20 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 5 times of water gaging cold preservations are spent the night or cold preservation 12 hours, goes precipitation, aqueous solution is regulated PH=3 with acid solution, cold preservation is spent the night or cold preservation 12 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with 2 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 4 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 2 times of column volumes, again with 2 times of column volume acid solution eluting, be eluted to partial neutral with 2 times of column volume neutral waters again, above eluent all discards; With 15 times of column volume 15% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 3g raw material/ml, adds 15 times of precipitation organic solvents, fully stirs, cold preservation 12 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 55, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 12.

Embodiment 6.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) gets raw material, extract 2 times with 90% alcohol reflux or dipping, add 5 times of amounts at every turn, perhaps with 90% ethanol percolate extraction of 15 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 1 times of water gaging cold preservation is spent the night or cold preservation 6 hours, goes precipitation, aqueous solution is regulated PH=2 with acid solution, cold preservation is spent the night or cold preservation 6 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with 1 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 2 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 1 times of column volume, again with 1 times of column volume acid solution eluting, be eluted to partial neutral with 1 times of column volume neutral water again, above eluent all discards; With 10 times of column volume 5% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 2g raw material/ml, adds 5 times of precipitation organic solvents, fully stirs, cold preservation 6 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 40, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 9.

In the present embodiment, step (1) and the described acid solution of step (2) are the hydrochloric acid solution of 0.01mol/L; The described alkaline solution of step (2) is 0.001% sodium hydroxide solution; The described dissolving of step (3) refers to 90% above concentration ethanol with organic solvent; The described precipitation organic solvent of step (3) is an acetone; The described organic solvent of step (4) is a n-butyl alcohol; Described mixed organic solvents is an ethyl acetate: acetone (7: 3); The described recrystallization organic solvent of step (4) is an ethyl acetate.

Embodiment 7.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) gets raw material, extract 4 times with 50% alcohol reflux or dipping, add 15 times of amounts at every turn, perhaps with 50% ethanol percolate extraction of 30 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 10 times of water gaging cold preservations are spent the night or cold preservation 24 hours, goes precipitation, aqueous solution is regulated PH=4 with acid solution, cold preservation is spent the night or cold preservation 24 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with 4 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 5 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 4 times of column volumes, again with 4 times of column volume acid solution eluting, be eluted to partial neutral with 4 times of column volume neutral waters again, above eluent all discards; With 20 times of column volume 30% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 5g raw material/ml, adds 20 times of precipitation organic solvents, fully stirs, cold preservation 24 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 70, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1:15.

In the present embodiment, step (1) and the described acid solution of step (2) are the sulfuric acid solution of 1mol/L; The described alkaline solution of step (2) is 1% aqua calcis; The described dissolving of step (3) refers to 90% above concentration dehydrated alcohol with organic solvent; The described precipitation organic solvent of step (3) is an ethyl acetate; The described organic solvent of step (4) is a chloroform; Described mixed organic solvents is an ethyl acetate: n-hexane (3: 7); The described recrystallization organic solvent of step (4) is a petroleum ether.

Embodiment 8.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) gets raw material, extract 2 times with 60% alcohol reflux or dipping, 8 times of amounts of each adding perhaps with 60% ethanol percolate extraction of 25 times of volumes, are collected extracting solution, be concentrated into and do not have the alcohol flavor, add that 3 times of water gaging cold preservations are spent the night or cold preservation 20 hours, go precipitation, aqueous solution regulates with acid solution that PH=2.5 cold preservation is spent the night or cold preservation 20 hours, go precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with 3 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 4 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 3 times of column volumes, again with 3 times of column volume acid solution eluting, be eluted to partial neutral with 3 times of column volume neutral waters again, above eluent all discards; With 12 times of column volume 10% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 4g raw material/ml, adds 10 times of precipitation organic solvents, fully stirs, cold preservation 20 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 50, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 10.

In the present embodiment, step (1) and the described acid solution of step (2) are the acetum of 0.05mol/L; The described alkaline solution of step (2) is 0.01% barium hydroxide or ammonia spirit; The described dissolving of step (3) refers to 90% above concentration methanol with organic solvent; The described precipitation organic solvent of step (3) is petroleum ether or n-hexane; The described organic solvent of step (4) is a dichloromethane; Described mixed organic solvents is a n-butyl alcohol: n-hexane (3: 7) or chloroform: n-hexane (3: 7) or dichloromethane: n-hexane (3: 7) or petroleum ether: n-hexane (3: 7) or petroleum ether: ethyl acetate (3: 7); The described recrystallization organic solvent of step (4) is a n-hexane.

Embodiment 9.A kind of butyl alcohol plant extract, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

(1) gets raw material, extract 3 times with 80% alcohol reflux or dipping, add 12 times of amounts at every turn, perhaps with 80% ethanol percolate extraction of 18 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 8 times of water gaging cold preservations are spent the night or cold preservation 15 hours, goes precipitation, aqueous solution is regulated PH=3.5 with acid solution, cold preservation is spent the night or cold preservation 15 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with 2.5 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 3 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 2.5 times of column volumes, again with 2.5 times of column volume acid solution eluting, be eluted to partial neutral with 2.5 times of column volume neutral waters again, above eluent all discards; With 18 times of column volume 25% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 4g raw material/ml, adds 12 times of precipitation organic solvents, fully stirs, cold preservation 8 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 55, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 11.

In the present embodiment, step (1) and the described acid solution of step (2) are the phosphoric acid solution of 0.1mol/L; The described alkaline solution of step (2) is 0.05% ethylenediamine solution or sodium carbonate liquor; The described dissolving of step (3) refers to 90% above concentration acetone with organic solvent; The described precipitation organic solvent of step (3) is n-butyl alcohol or dichloromethane; The described organic solvent of step (4) is a petroleum ether; Described mixed organic solvents is an ethyl acetate: n-hexane (7: 3) or n-butyl alcohol: n-hexane (7: 3) or chloroform: n-hexane (7: 3) or dichloromethane: n-hexane (7: 3) or petroleum ether: n-hexane (7: 3) or petroleum ether: ethyl acetate (7: 3); The described recrystallization organic solvent of step (4) is a dichloromethane.

Embodiment 10.A kind of butyl alcohol by-product salidroside plant extraction, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

Embodiment 11.A kind of butyl alcohol by-product salidroside plant extraction, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(1) gets raw material, extract 2 times with 50% alcohol reflux or dipping, add 5 times of amounts at every turn, perhaps with 50% ethanol percolate extraction of 15 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 1 times of water gaging cold preservation is spent the night or cold preservation 6 hours, goes precipitation, aqueous solution is regulated PH=2 with acid solution, cold preservation is spent the night or cold preservation 6 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 2g raw material/ml, adds 5 times of precipitation organic solvents, fully stirs, cold preservation 6 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 40, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 9;

In the present embodiment, step (1) and the described acid solution of step (2) are hydrochloric acid solution or sulfuric acid solution or acetum or the phosphoric acid solution of 0.2mol/L; The described alkaline solution of step (2) is 0.1% solution of potassium carbonate or sodium bicarbonate solution; The described dissolving of step (3) refers to 90% above concentration n-butyl alcohol with organic solvent; The described precipitation organic solvent of step (3) is dichloromethane or chloroform; The described organic solvent of step (4) is a petroleum ether; Listening the mixed organic solvents of stating is ethyl acetate: n-hexane (4: 6) or n-butyl alcohol: n-hexane (4: 6) or chloroform: n-hexane (4: 6) or dichloromethane: n-hexane (4: 6) or petroleum ether: n-hexane (4: 6) or petroleum ether: ethyl acetate (4: 6); The described recrystallization organic solvent of step (4) is a chloroform.

Embodiment 12.A kind of butyl alcohol by-product salidroside plant extraction, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(1) gets raw material, extract 4 times with 90% alcohol reflux or dipping, add 15 times of amounts at every turn, perhaps with 90% ethanol percolate extraction of 30 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 10 times of water gaging cold preservations are spent the night or cold preservation 24 hours, goes precipitation, aqueous solution is regulated PH=4 with acid solution, cold preservation is spent the night or cold preservation 24 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 5g raw material/ml, adds 20 times of precipitation organic solvents, fully stirs, cold preservation 24 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 70, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 15;

In the present embodiment, step (1) and the described acid solution of step (2) are hydrochloric acid solution, sulfuric acid solution, acetum or the phosphoric acid solution of 0.3mol/L; The described alkaline solution of step (2) is that 0.2% potassium hydroxide solution or other are not the aqueous solution of the alkaline matter of resin absorption; The described dissolving of step (3) refers to 90% above concentration n-butyl alcohol with organic solvent; The described precipitation organic solvent of step (3) is ether or other weakly polar organic solvent; The described organic solvent of step (4) is a n-butyl alcohol; Described mixed organic solvents is an ethyl acetate: acetone (9: 1) or ethyl acetate: n-hexane (5: 5) or n-butyl alcohol: n-hexane (5: 5) or chloroform: n-hexane (5: 5) or dichloromethane: n-hexane (5: 5) or petroleum ether: n-hexane (5: 5) or petroleum ether: ethyl acetate (5: 5); The described recrystallization organic solvent of step (4) is an ethyl acetate.

Embodiment 13.A kind of butyl alcohol by-product salidroside plant extraction, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(2) closely neutral with 2 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 3 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 2 times of column volumes, again with 2 times of column volume acid solution eluting, be eluted to partial neutral with 2 times of column volume neutral waters again, above eluent all discards; With 15 times of column volume 20% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 3g raw material/ml, adds 15 times of precipitation organic solvents, fully stirs, cold preservation 12 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 55, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1:12:

In the present embodiment, step (1) and the described acid solution of step (2) are hydrochloric acid solution, sulfuric acid solution, acetum or the phosphoric acid solution of 0.5mol/L; The described alkaline solution of step (2) is that 0.3% sodium hydroxide solution, aqua calcis, barium hydroxide, ammonia spirit, ethylenediamine solution, sodium carbonate liquor, solution of potassium carbonate, sodium bicarbonate solution, potassium bicarbonate solution, potassium hydroxide solution or other are not the aqueous solution of the alkaline matter of resin absorption; The described dissolving of step (3) refers to the ethyl acetate of 90% above concentration with organic solvent; The described precipitation organic solvent of step (3) is acetone, ethyl acetate, petroleum ether, n-hexane, n-butyl alcohol, dichloromethane, chloroform, ether or other weakly polar organic solvent; The described organic solvent of step (4) is n-butyl alcohol, chloroform, dichloromethane or petroleum ether; Described mixed organic solvents is an ethyl acetate: acetone (8: 2) or ethyl acetate: n-hexane (4: 5) or n-butyl alcohol: n-hexane (4: 5) or chloroform: n-hexane (4: 5) or dichloromethane: n-hexane (4: 5) or petroleum ether: n-hexane (4: 5) or petroleum ether: ethyl acetate (4: 5); The described recrystallization organic solvent of step (4) is ethyl acetate, petroleum ether, n-hexane, dichloromethane or chloroform.

Embodiment 14.A kind of butyl alcohol by-product salidroside plant extraction, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(1) gets raw material, extract 2 times with 60% alcohol reflux or dipping, add 8 times of amounts at every turn, perhaps with 60% ethanol percolate extraction of 25 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 3 times of water gaging cold preservations are spent the night or cold preservation 20 hours, goes precipitation, aqueous solution is regulated PH=2.5 with acid solution, cold preservation is spent the night or cold preservation 20 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 4g raw material/ml, adds 10 times of precipitation organic solvents, fully stirs, cold preservation 20 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 50, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 10;

In the present embodiment, step (1) and the described acid solution of step (2) are hydrochloric acid solution, sulfuric acid solution, acetum or the phosphoric acid solution of 0.7mol/L; The described alkaline solution of step (2) is that 0.6% sodium hydroxide solution, aqua calcis, barium hydroxide, ammonia spirit, ethylenediamine solution, sodium carbonate liquor, solution of potassium carbonate, sodium bicarbonate solution, potassium bicarbonate solution, potassium hydroxide solution or other are not the aqueous solution of the alkaline matter of resin absorption; The described dissolving of step (3) refers to 90% above concentration ethanol, dehydrated alcohol, methanol, acetone, n-butyl alcohol or ethyl acetate with organic solvent; The described precipitation organic solvent of step (3) is acetone, ethyl acetate, petroleum ether, n-hexane, n-butyl alcohol, dichloromethane, chloroform, ether or other weakly polar organic solvent; The described organic solvent of step (4) is n-butyl alcohol, chloroform, dichloromethane or petroleum ether; Described mixed organic solvents is an ethyl acetate: acetone (7: 2); The described recrystallization organic solvent of step (4) is ethyl acetate, petroleum ether, n-hexane, dichloromethane or chloroform.

Embodiment 15.A kind of butyl alcohol by-product salidroside plant extraction, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

(1) gets raw material, extract 3 times with 80% alcohol reflux or dipping, add 12 times of amounts at every turn, perhaps with 80% ethanol percolate extraction of 18 times of volumes, collect extracting solution, being concentrated into does not have the alcohol flavor, adds that 8 times of water gaging cold preservations are spent the night or cold preservation 10 hours, goes precipitation, aqueous solution is regulated PH=3 with acid solution, cold preservation is spent the night or cold preservation 10 hours, goes precipitation, low pole macroporous resin on the clear liquor;

(2) closely neutral with 2 times of column volume deionized water eluting macroporous resin column earlier to effluent, again with 2 times of column volume alkaline solution eluting macroporous resin column, and then wash nearly neutrality with 2 times of column volumes, again with 2 times of column volume acid solution eluting, be eluted to partial neutral with 2 times of column volume neutral waters again, above eluent all discards; With 12 times of column volume 25% ethanol elutions, collect eluent again;

(3) eluent is concentrated, insoluble matter is removed with the dissolving organic solvent dissolution in dry back, clear liquor is concentrated into and is equivalent to 5g raw material/ml, adds 18 times of precipitation organic solvents, fully stirs, cold preservation 10 hours, go precipitation, after getting clear liquor and concentrating, add chromatographic silica gel and mix sample, silica gel and raw-material weight ratio are: 1: 65, evaporate to dryness, last silica gel column chromatography, silicagel column blade diameter length ratio are 1: 13;

In the present embodiment, step (1) and the described acid solution of step (2) are hydrochloric acid solution, sulfuric acid solution, acetum or the phosphoric acid solution of 0.9mol/L; The described alkaline solution of step (2) is that 0.8% sodium hydroxide solution, aqua calcis, barium hydroxide, ammonia spirit, ethylenediamine solution, sodium carbonate liquor, solution of potassium carbonate, sodium bicarbonate solution, potassium bicarbonate solution, potassium hydroxide solution or other are not the aqueous solution of the alkaline matter of resin absorption; The described dissolving of step (3) refers to 90% above concentration ethanol, dehydrated alcohol, methanol, acetone, n-butyl alcohol or ethyl acetate with organic solvent; The described precipitation organic solvent of step (3) is acetone, ethyl acetate, petroleum ether, n-hexane, n-butyl alcohol, dichloromethane, chloroform, ether or other weakly polar organic solvent; The described organic solvent of step (4) is n-butyl alcohol, chloroform, dichloromethane or petroleum ether; Described mixed organic solvents is an ethyl acetate: acetone (9: 3); The described recrystallization organic solvent of step (4) is ethyl acetate, petroleum ether, n-hexane, dichloromethane or chloroform.

Embodiment 16.A kind of butyl alcohol extract formulation, it is the tablet that embodiment 1 described butyl alcohol plant extract and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 17.A kind of butyl alcohol extract formulation, it is the capsule that embodiment 4 described butyl alcohol plant extracts and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 18.A kind of butyl alcohol extract formulation, it is the granule that embodiment 5 described butyl alcohol plant extracts and conventional medicine carrier are made.

Embodiment 19.A kind of butyl alcohol extract formulation, it is the infusion solution that embodiment 6 described butyl alcohol plant extracts and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 20.A kind of butyl alcohol extract formulation, it is the injectable powder that embodiment 7 described butyl alcohol plant extracts and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 21.A kind of butyl alcohol extract formulation, it is the injection that embodiment 8 described butyl alcohol plant extracts and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 22.A kind of butyl alcohol extract formulation, it is the controlled release preparation that embodiment 9 described butyl alcohol plant extracts and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 23.In embodiment 16, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 0.1%.

Embodiment 24.In embodiment 17, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 99.5%.

Embodiment 25.In embodiment 18, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 0.5%.

Embodiment 26.In embodiment 19, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 95%.

Embodiment 27.In embodiment 20, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 90%.

Embodiment 28.In embodiment 21, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 80%.

Embodiment 29.In embodiment 22, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 70%.

Embodiment 30.In embodiment 16, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 60%.

Embodiment 31.In embodiment 17, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 50%.

Embodiment 32.In embodiment 18, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 40%.

Embodiment 33.In embodiment 19, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 30%.

Embodiment 34.In embodiment 20, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 20%.

Embodiment 35.In embodiment 21, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 10%.

Embodiment 36.In embodiment 22, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 1%.

Embodiment 37.A kind of butyl alcohol salidroside plant extraction preparation, it is the tablet that embodiment 10 described butyl alcohol plant extracts and salidroside plant extraction and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 38.A kind of butyl alcohol salidroside plant extraction preparation, it is the granule that embodiment 11 described butyl alcohol plant extracts and salidroside plant extraction and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 39.A kind of butyl alcohol by-product salidroside plant extraction preparation, it is the oral liquid that embodiment 12 described butyl alcohol plant extracts and salidroside plant extraction and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 40.A kind of butyl alcohol salidroside plant extraction preparation, it is the capsule that embodiment 13 described butyl alcohol plant extracts and salidroside plant extraction and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 41.A kind of butyl alcohol salidroside plant extraction preparation, it is the injection that embodiment 14 described butyl alcohol plant extracts and salidroside plant extraction and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 42.A kind of butyl alcohol salidroside plant extraction preparation, it is the pill that embodiment 15 described butyl alcohol plant extracts and salidroside plant extraction and conventional medicine carrier are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 43.In embodiment 37, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 0.1.

Embodiment 44.In embodiment 38, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 10.

Embodiment 45.In embodiment 39, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 1.

Embodiment 46.In embodiment 40, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 3.

Embodiment 47.In embodiment 41, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 5.

Embodiment 48.In embodiment 42, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 7.

Embodiment 49.In embodiment 37, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 9.

Embodiment 50.In embodiment 38, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 8.

Embodiment 51.In embodiment 39, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 6.

Embodiment 52.In embodiment 40, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 4.

Embodiment 53.In embodiment 41, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 2.

Embodiment 54.In embodiment 42, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 0.5.

Embodiment 55.A kind of butyl alcohol plant extract preparation, it is to get the butyl alcohol plant extract adding filler of embodiment 1, tablet or the capsule that the disintegrating agent assembly is made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 56.A kind of butyl alcohol plant extract preparation, it is to get the butyl alcohol plant extract adding filler of embodiment 4 and slow releasing tablet or the capsule that hypromellose K4M assembly is made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 57.A kind of butyl alcohol plant extract preparation, it is that the butyl alcohol plant extract of getting embodiment 5 is scattered in and obtains soft capsule in the oil phase; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 58.A kind of butyl alcohol plant extract preparation, it is to get the butyl alcohol plant extract adding solubilizing agent of embodiment 6 or the injection that cosolvent forms; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 59.A kind of butyl alcohol plant extract preparation, it is that the butyl alcohol plant extract of getting embodiment 7 is scattered in the injectable emulsion that obtains in the oil phase; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 60.A kind of butyl alcohol plant extract preparation, it is that the butyl alcohol plant extract of getting embodiment 8 adds suspension type injection or the lyophilizing injection powder pin that pharmaceutic adjuvant is made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 61.A kind of butyl alcohol and salidroside plant extraction preparation, it is to get the butyl alcohol plant extract of embodiment 10 and tablet or the capsule that salidroside plant extraction adding filler, disintegrating agent assembly are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 62.A kind of butyl alcohol and salidroside plant extraction preparation, it is to get the butyl alcohol plant extract of embodiment 11 and salidroside plant extraction to add slow releasing tablet or the capsule that filler and hypromellose K4M assembly are made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 63.A kind of butyl alcohol and salidroside plant extraction preparation, it is to get the butyl alcohol plant extract of embodiment 12 and salidroside plant extraction to be scattered in and to obtain soft capsule in the oil phase; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 64.A kind of butyl alcohol and salidroside plant extraction preparation, it is to get the butyl alcohol plant extract of embodiment 13 and the injection that salidroside plant extraction adds solubilizing agent or cosolvent formation; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 65.A kind of butyl alcohol and salidroside plant extraction preparation, it is that butyl alcohol plant extract and the salidroside plant extraction of getting embodiment 14 are scattered in the injectable emulsion that obtains in the oil phase; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 66.A kind of butyl alcohol and salidroside plant extraction preparation, it is that butyl alcohol plant extract and the salidroside plant extraction of getting embodiment 15 add suspension type injection or the lyophilizing injection powder pin that pharmaceutic adjuvant is made; Be used for the treatment of hepatocarcinoma, pulmonary carcinoma and other tumors, perhaps be used for the treatment of coronary heart disease, cerebral infarction, viral myocarditis and senile dementia.

Embodiment 67.Filler described in the embodiment 55,56,61,62 is selected from lactose, microcrystalline Cellulose, dextrin, starch or calcium phosphate; Disintegrating agent described in the embodiment 55,61 is selected from hyprolose, carboxymethyl starch sodium, polyvinylpolypyrrolidone or cross-linking sodium carboxymethyl cellulose; Also optional binding agent, lubricant or the wetting agent of adding when film-making agent and capsule.

Embodiment 68.Oil phase described in the embodiment 57,63 is selected from soybean oil, PEG400, Oleum Gossypii semen, Oleum Arachidis hypogaeae semen, Oleum Sesami, Semen Maydis oil or olive oil; When the system soft capsule, also can add solubilizing agent or cosolvent and antioxidant etc.

Embodiment 69.Used solubilizing agent is selected from polyoxyethylene ether Oleum Ricini, tween or pluronic F-68 among the embodiment 58,64; Cosolvent can be selected basic auxiliary for use, and as basic amino acids such as arginine, lysine, meglumine, perhaps alcohols comprises ethanol, propylene glycol and mannitol.

Embodiment 70.The preparation method of the suspension type injection described in the embodiment 60,66 is, get the butyl alcohol plant extract, perhaps butyl alcohol and salidroside plant extraction, after the adding polyoxyethylene sorbitan monoleate mixes and grinds, be dissolved into the aqueous solution of phosphoric acid potassium dihydrogen, dipotassium hydrogen phosphate, Nipagin ester and sodium carboxymethyl cellulose, make through grinding.

Claims

1. a butyl alcohol plant extract is characterized in that, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

2. a kind of butyl alcohol plant extract according to claim 1 is characterized in that, it is from the root or the rhizome of Radix Rhodiolae, perhaps extracts from the mature fruit of Fructus Ligustri Lucidi or the bean sauce after flower or the fermentation, acid-sludge, and its extraction step is as follows,

3. a butyl alcohol by-product salidroside plant extraction is characterized in that, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

4. a kind of butyl alcohol by-product salidroside plant extraction according to claim 3 is characterized in that, it is from the root or the rhizome of Radix Rhodiolae, and perhaps from the mature fruit of Fructus Ligustri Lucidi or extraction spending, its extraction step is as follows,

5. according to any one described a kind of butyl alcohol plant extract or butyl alcohol by-product salidroside plant extraction among the described 1-4 of claim, it is characterized in that step (1) and the described acid solution of step (2) are hydrochloric acid solution, sulfuric acid solution, acetum or the phosphoric acid solution of 0.01～1mol/L; The described alkaline solution of step (2) is that 0.001%～1% sodium hydroxide solution, aqua calcis, barium hydroxide, ammonia spirit, ethylenediamine solution, sodium carbonate liquor, solution of potassium carbonate, sodium bicarbonate solution, potassium bicarbonate solution, potassium hydroxide solution or other are not the aqueous solution of the alkaline matter of resin absorption; The described dissolving of step (3) refers to 90% above concentration ethanol, dehydrated alcohol, methanol, acetone, n-butyl alcohol or ethyl acetate with organic solvent; The described precipitation organic solvent of step (3) is acetone, ethyl acetate, petroleum ether, normal hexane, n-butyl alcohol, dichloromethane, chloroform, ether or other weakly polar organic solvent; The described organic solvent of step (4) is n-butyl alcohol, chloroform, dichloromethane or petroleum ether; 7～3) or petroleum ether 7～3), petroleum ether 7～3), dichloromethane 7～3), chloroform 7～3), n-butyl alcohol 3～1), ethyl acetate described mixed organic solvents is an ethyl acetate: acetone (7～9:: normal hexane (3～7:: normal hexane (3～7:: normal hexane (3～7:: normal hexane (3～7:: normal hexane (3～7:: ethyl acetate (3～7: 7～3); The described recrystallization organic solvent of step (4) is ethyl acetate, petroleum ether, normal hexane, dichloromethane or chloroform.

6. the butyl alcohol extract formulation described in claim 1 or 2 is characterized in that, it be the butyl alcohol plant extract and pharmaceutically the acceptable pharmaceutical carrier make the medicament of acceptable any dosage form on the pharmaceutics.

7. a kind of butyl alcohol extract formulation according to claim 6 is characterized in that, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 0.1%-99.5%.

8. a kind of butyl alcohol extract formulation according to claim 6 is characterized in that, in butyl alcohol plant extract and pharmaceutical carrier raw material, the weight percent content of extract is 0.5%-95%.

9. one kind as claim 3 or 4 described a kind of butyl alcohol by-product salidroside plant extraction preparations, it is characterized in that, it be butyl alcohol plant extract and salidroside plant extraction and pharmaceutically the acceptable pharmaceutical carrier make the medicament of acceptable any dosage form on the pharmaceutics.

10. preparation according to claim 9 is characterized in that, wherein, butyl alcohol plant extract and salidroside plant extraction weight ratio are 1: 0.1～10.

11. the application of a kind of butyl alcohol by-product salidroside plant extraction preparation described in a kind of butyl alcohol plant extract preparation described in claim 1 or 2 described a kind of butyl alcohol plant extracts or the claim 5 or a kind of butyl alcohol by-product salidroside plant extraction described in the claim 3 or the claim 9 in the medicine of preparation treatment hepatocarcinoma, pulmonary carcinoma and other tumors, the perhaps application in preparation treatment coronary heart disease, cerebral infarction, viral myocarditis and alzheimer disease medicine.