CN101139577B - Glucoamylase produced by fermentation of wine lees miscible liquid and method for producing alcohol by fermenting restaurant garbage with this glucoamylase - Google Patents
Glucoamylase produced by fermentation of wine lees miscible liquid and method for producing alcohol by fermenting restaurant garbage with this glucoamylase Download PDFInfo
- Publication number
- CN101139577B CN101139577B CN2007100727054A CN200710072705A CN101139577B CN 101139577 B CN101139577 B CN 101139577B CN 2007100727054 A CN2007100727054 A CN 2007100727054A CN 200710072705 A CN200710072705 A CN 200710072705A CN 101139577 B CN101139577 B CN 101139577B
- Authority
- CN
- China
- Prior art keywords
- saccharifying enzyme
- alcohol
- liquid
- vinasse
- mixed solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention provides a saccharifying enzyme produced by fermenting draff mixed fluid and a method for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish, which relates to a method for producing saccharifying enzyme and a way for producing alcohol by using the saccharifying enzyme to ferment kitchen and food rubbish. The method for producing the saccharifyingenzyme comprises the following procedures: I. mixing draff fluid with water, adding solid draff; II. disinfecting, adding aspergillosis fluid to ferment. The method for producing alcohol: a. mixing kitchen and food rubbish with waste water from a dining hall or tap water; b. adding saccharifying enzyme produced by above method, inoculating saccharomycetes, then fermenting under an anaerobic atmosphere; c. distilling the alcohol. The invention provides nitrous substances necessary for microbes to grow by using draff fluid and solid draff, which can act as traditional enzyme-producing culture medium. The invention can improve the activity of the saccharifying enzyme, and the produced saccharifying enzyme can be directly used for alcohol fermenting kitchen and food rubbish without purifying and separating, this can not only reduce the production cost for alcohol, but also change waste into treasure, improve the added value of kitchen and food rubbish, and avoid pollution from draff and draff fluid on environment.
Description
Technical field
The present invention relates to a kind of method of producing the method for saccharifying enzyme and utilizing this saccharifying enzyme fermented garbage from kitchen production alcohol.
Background technology
Outstanding day by day along with energy problem, the research of the alternative energy obtains paying attention to day by day, and wherein fuel alcohol has obtained fast development, and people have carried out the research that plurality of raw materials is produced fuel alcohol, as cellulosic material, grain in storage for years, molasses etc.Publication number is that the patent of CN1948498 has been announced a kind of method of utilizing changing food waste to produce alcohol fuel, though have the advantage of price on the raw material, needs to use industrial enzyme in process of production, causes the production cost height.
This biomass energy of development fuel alcohol has also been brought problem to society and has been troubled, particularly produce remaining using solid distillers ' grains and liquid poor liquid in the production process, because its quantity discharged is big, COD content height, and is huge to the harm of environment, restricted the development of fuel alcohol.Therefore, the recycling treatment for vinasse also needs to be resolved hurrily.
Summary of the invention
The present invention is in order to solve the high and vinasse that produce of existing preparation alcohol method cost, the problem of poor liquid environmental pollution, and has proposed that saccharifying enzyme is produced in a kind of vinasse mixed solution fermentation and with the method for the saccharifying enzyme fermented garbage from kitchen production alcohol of this method output.
The method that saccharifying enzyme is produced in the fermentation of vinasse mixed solution realizes by following steps: one, the mass ratio with 1～2:1 mixes the poor liquid of vinasse with water, and to add 0.5～1.5% (w/w), water ratio in mixed solution be 55～70% using solid distillers ' grains, obtains the vinasse mixed solution; Two, the vinasse mixed solution is sterilized under 115～121 ℃ of conditions 15～20min, to insert spore concentration be 1 * 10 to the ratio of adding 6～10ml aspergillus tubigensis liquid in 100ml vinasse mixed solution then
7～3 * 10
7Individual/the ml aspergillus tubigensis, shake flask fermentation 84～96h under 30～35 ℃, the condition of 100～200r/min can obtain saccharifying enzyme liquid.
The method of producing alcohol with the saccharifying enzyme fermented garbage from kitchen of aforesaid method output realizes by following steps: a, the changing food waste after will pulverizing and dining room waste water or tap water mix by the mass ratio of 1～4:1, obtain the changing food waste fermention medium; Adding saccharifying enzyme work in b, the every gram changing food waste fermention medium is the saccharifying enzyme liquid of the aforesaid method output of 90～100U, and add the saccharomycetic ratio of 8～10ml according to 100ml changing food waste fermention medium and insert yeast, anaerobically fermenting 60～80 hours under 30～35 ℃ condition then; C, distillation fermented liquid promptly obtain alcohol.
Changing food waste is produced the organic content height of the poor liquid of alcohol residue vinasse, its COD value can reach 50000mg/L, and the nitrogen content height of using solid distillers ' grains, its crude protein reaches more than 35%, also contain nutritive elements such as metal ion simultaneously in the changing food waste vinasse, can fully satisfy the growth requirement of zymogenic bacteria.Therefore, the present invention provides microorganism growth required nutritive substance with poor liquid of vinasse and using solid distillers ' grains, can play tradition and produce the effect of enzyme substratum, not only can improve saccharifying enzyme lives, and the saccharifying enzyme that produces does not need purifies and separates directly to can be used for the zymamsis of changing food waste, this not only can make the Alcohol Production cost reduce, and can reach the added value that turns waste into wealth, improves changing food waste, the purpose of avoiding vinasse and poor liquid that environment is polluted.
Embodiment
Embodiment one: the method that saccharifying enzyme is produced in the poor liquid fermentation of present embodiment vinasse realizes by following steps: one, the mass ratio with 1～2:1 mixes the poor liquid of vinasse with water, and to add 0.5～1.5% (w/w), water ratio in mixed solution be 55～70% using solid distillers ' grains, obtains the vinasse mixed solution; Two, the vinasse mixed solution is sterilized under 115～121 ℃ of conditions 15～20min, to insert spore concentration be 1 * 10 to the ratio of adding 6～10ml aspergillus tubigensis liquid in 100ml vinasse mixed solution then
7～3 * 10
7Individual/the ml aspergillus tubigensis, shake flask fermentation 84～96h under 30～35 ℃, the condition of 100～200r/min can obtain saccharifying enzyme liquid.
The contrast experiment:
Mass ratio with 1～2:1 mixes the vinasse waste liquid with water, add wheat bran, vinasse (water ratio 70%) and the distiller's dried grain of mixed solution weight 0.8% respectively, wherein wheat bran is market sale, and distiller's dried grain then is that the vinasse that use among the present invention are dried the moisture gained through 105 ℃; The 20min that under 121 ℃ of conditions, sterilizes, the ratio of adding 10ml black-koji mould liquid according to 100ml vinasse mixed solution inserts aspergillus, shake flask fermentation 84～96h under 30～32 ℃, the condition of 200r/min then, other condition and parameter are all identical.Experimental result is as shown in table 1:
Table 1
Experimental data shows that the vinasse after use distillation, the solid-liquid separation are more suitable for as the saccharifying enzyme fermentation substrate.
Embodiment two: the difference of present embodiment and embodiment one is to add in the step 1 0.8～1.2% (w/w), water ratio in mixed solution be 58～67% using solid distillers ' grains.Other step is identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is to add in the step 1 1% (w/w), water ratio in mixed solution be 60% using solid distillers ' grains.Other step is identical with embodiment.
Embodiment four: the difference of present embodiment and the embodiment one 17～18min that is in the step 2 vinasse mixed solution sterilized under 120 ℃ of conditions.Other step is identical with embodiment one.
Embodiment five: the difference of present embodiment and embodiment one is that the ratio access spore concentration in 100ml vinasse mixed solution interpolation 7～9ml aspergillus tubigensis liquid is 1 * 10 in the step 2
7～2 * 10
7Individual/the ml aspergillus tubigensis, shake flask fermentation 88～92h under 30～35 ℃, the condition of 120～180r/min.Other step is identical with embodiment one.
Embodiment six: the difference of present embodiment and embodiment one is that the poor liquid of vinasse distills out alcohol after the liquid substance that solid-liquid separation obtains for being that raw material carries out zymamsis with the changing food waste in the step 1.Other step is identical with embodiment one.
Embodiment seven: the difference of present embodiment and embodiment one is that the using solid distillers ' grains that adds to the poor liquid of vinasse in the step 1 is to be that raw material carries out zymamsis with the changing food waste, distills out alcohol after the solid matter that solid-liquid separation obtains.Other step is identical with embodiment one.
Embodiment eight: the difference of present embodiment and embodiment one is that the aspergillus of step 2 inoculation is aspergillus niger Aspergillus niger4309 or Aspergillus usamii Aspergillus usamii.Other step is identical with embodiment one.
Embodiment nine: the method that saccharifying enzyme is produced in the poor liquid fermentation of present embodiment vinasse realizes by following steps: one, the mass ratio with 2:1 mixes the poor liquid of vinasse with water, and to add 1% (w/w), water ratio in mixed solution be 60% using solid distillers ' grains, obtains the vinasse mixed solution; Two, the vinasse mixed solution is sterilized under 121 ℃ of conditions 20min, to insert spore concentration be 2 * 10 to the ratio of adding 8ml aspergillus tubigensis liquid in 100ml vinasse mixed solution then
7Individual/the ml aspergillus tubigensis, shake flask fermentation 90h under 34 ℃, the condition of 150r/min can obtain saccharifying enzyme liquid; Wherein the poor liquid of vinasse distills out alcohol after the liquid substance that solid-liquid separation obtains for being that raw material carries out zymamsis with the changing food waste in the step 1; The using solid distillers ' grains that adds to the poor liquid of vinasse in the step 1 is to be that raw material carries out zymamsis with the changing food waste, distills out alcohol after the solid matter that solid-liquid separation obtains; The aspergillus of rapid two inoculations is aspergillus niger Aspergillus niger4309.
The saccharifying enzyme thinning enzyme that present embodiment obtains is lived and is 2850U/ml.
Embodiment ten: the method that present embodiment utilizes the saccharifying enzyme fermented garbage from kitchen of embodiment one method output to produce alcohol realizes by following steps: a, the changing food waste after will pulverizing and dining room waste water or tap water mix by the mass ratio of 1～4:1, obtain the changing food waste fermention medium; Adding saccharifying enzyme work in b, the every gram changing food waste fermention medium is the saccharifying enzyme liquid of embodiment one output of 90～100U, and add the saccharomycetic ratio of 8～10ml according to 100ml changing food waste fermention medium and insert yeast, anaerobically fermenting 60～80 hours under 30～35 ℃ condition then; C, distillation fermented liquid promptly obtain alcohol.
The contrast experiment:
Changing food waste after pulverizing and dining room waste water or the tap water mass ratio by 2:1 is mixed, the saccharifying enzyme liquid and the industrial saccharifying enzyme that add the embodiment one method output of the enzyme 100U of being alive in every gram changing food waste fermention medium respectively, and insert the yeast of changing food waste fermention medium quality 10%, anaerobically fermenting 60 hours under 35 ℃ condition then.Add after testing that ethanol concn is 37g/L in the fermented liquid of saccharifying enzyme liquid of embodiment one method output, add that ethanol concn is 40g/L in the fermented liquid of industrial saccharifying enzyme, prove that the saccharifying enzyme of embodiment one method output can substitute industrial saccharifying enzyme fully.
Embodiment 11: the changing food waste after present embodiment and the difference of embodiment ten are to pulverize among the step a and dining room waste water or tap water mix by the mass ratio of 1.2～3:1.Other step is identical with embodiment ten.
Embodiment 12: the difference of present embodiment and embodiment ten is to add in every gram changing food waste fermention medium among the step b saccharifying enzyme liquid of embodiment one output of 95U, and adds the saccharomycetic ratio of 9ml according to 100ml changing food waste fermention medium and insert yeast.Other step is identical with embodiment ten.
Embodiment 13: the difference of present embodiment and embodiment ten is step b anaerobically fermenting 64～72 hours.Other step is identical with embodiment ten.
Embodiment 14: the changing food waste after present embodiment and the difference of embodiment ten are to pulverize among the step a and dining room waste water or tap water mix by the mass ratio of 1.5:1.Other step is identical with embodiment ten.
Claims (8)
1. the vinasse mixed solution ferments and produces the method for saccharifying enzyme, it is characterized in that this method realizes by following steps: will be that raw material carries out zymamsis, distills out alcohol after the poor liquid of the vinasse that solid-liquid separation obtains mixes with water with the changing food waste one, with 1: 1 to 2: 1 mass ratio, and in mixed solution, add 0.5%～1.5% (w/w), water ratio be 55%～70% be that raw material carries out zymamsis, distills out alcohol after the using solid distillers ' grains that solid-liquid separation obtains obtains the vinasse mixed solution with the changing food waste; Two, the vinasse mixed solution is sterilized under 115～121 ℃ of conditions 15～20min, to insert spore concentration be 1 * 10 to the ratio of adding 6～10ml aspergillus tubigensis liquid in 100ml vinasse mixed solution then
7～3 * 10
7Individual/the ml aspergillus tubigensis, shake flask fermentation 84～96h under 30～35 ℃, the condition of 100～200r/min can obtain saccharifying enzyme liquid.
2. the method that saccharifying enzyme is produced in vinasse mixed solution according to claim 1 fermentation, 20min is characterized in that in the step 2 vinasse mixed solution sterilized under 121 ℃ of conditions.
3. the method that saccharifying enzyme is produced in vinasse mixed solution fermentation according to claim 1 is characterized in that the ratio access spore concentration in 100ml vinasse mixed solution interpolation 7～9ml aspergillus tubigensis liquid is 1 * 10 in the step 2
7～2 * 10
7Individual/the ml aspergillus tubigensis, shake flask fermentation 88～92h under 30～35 ℃, the condition of 120～180r/min.
4. the method that saccharifying enzyme is produced in the poor liquid fermentation of vinasse according to claim 1 is characterized in that the aspergillus of step 2 inoculation is aspergillus niger (Aspergillus niger) 4309 or Aspergillus usamii (Aspergillususamii).
5. use the method for the saccharifying enzyme fermented garbage from kitchen production alcohol of claim 1 method output, it is characterized in that present method realizes by following steps: a, the changing food waste after will pulverizing and dining room waste water or tap water mix by 1: 1 to 4: 1 mass ratio, obtain the changing food waste fermention medium; Adding saccharifying enzyme work in b, the every gram changing food waste fermention medium is the saccharifying enzyme liquid of the claim 1 method output of 90～100U, and add the saccharomycetic ratio of 8～10ml according to 100ml changing food waste fermention medium and insert yeast, anaerobically fermenting 60～80 hours under 30～35 ℃ condition then; C, distillation fermented liquid promptly obtain alcohol.
6. saccharifying enzyme fermented garbage from kitchen according to claim 5 is produced the method for alcohol, and the changing food waste after it is characterized in that pulverizing among the step a and dining room waste water or tap water mix by 1.2: 1 to 3: 1 mass ratio.
7. saccharifying enzyme fermented garbage from kitchen according to claim 5 is produced the method for alcohol, it is characterized in that adding in every gram changing food waste fermention medium among the step b saccharifying enzyme liquid of 95U, add the saccharomycetic ratio of 9ml according to 100ml changing food waste fermention medium simultaneously and insert yeast by claim 1 method output.
8. saccharifying enzyme fermented garbage from kitchen according to claim 5 is produced the method for alcohol, it is characterized in that step b anaerobically fermenting 64～72 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100727054A CN101139577B (en) | 2007-08-24 | 2007-08-24 | Glucoamylase produced by fermentation of wine lees miscible liquid and method for producing alcohol by fermenting restaurant garbage with this glucoamylase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100727054A CN101139577B (en) | 2007-08-24 | 2007-08-24 | Glucoamylase produced by fermentation of wine lees miscible liquid and method for producing alcohol by fermenting restaurant garbage with this glucoamylase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101139577A CN101139577A (en) | 2008-03-12 |
| CN101139577B true CN101139577B (en) | 2010-06-30 |
Family
ID=39191681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100727054A Expired - Fee Related CN101139577B (en) | 2007-08-24 | 2007-08-24 | Glucoamylase produced by fermentation of wine lees miscible liquid and method for producing alcohol by fermenting restaurant garbage with this glucoamylase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101139577B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865901A (en) * | 2014-04-08 | 2014-06-18 | 大连工业大学 | Fermentation culture medium for glucoamylase and glucoamylase fermentation method |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR102012031841A2 (en) * | 2012-12-13 | 2014-09-23 | Braerg Grupo Brasileiro De Pesquisas Especializadas Ltda | PROCESS FOR OBTAINING BIOFUEL FROM LIGNOCELLULOSTIC AND / OR AMILACEOUS BIOMASS |
| CN115254920A (en) * | 2022-08-16 | 2022-11-01 | 福建省恒创环保科技有限公司 | Harmless treatment process for kitchen garbage |
-
2007
- 2007-08-24 CN CN2007100727054A patent/CN101139577B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865901A (en) * | 2014-04-08 | 2014-06-18 | 大连工业大学 | Fermentation culture medium for glucoamylase and glucoamylase fermentation method |
| CN103865901B (en) * | 2014-04-08 | 2015-10-14 | 大连工业大学 | A kind of fermention medium of saccharifying enzyme and fermentation process thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101139577A (en) | 2008-03-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Deenanath et al. | The bioethanol industry in Sub-Saharan Africa: history, challenges, and prospects | |
| Li et al. | Acetic acid production from food wastes using yeast and acetic acid bacteria micro-aerobic fermentation | |
| CN101864362B (en) | Compound microbial bacterial preparation and application thereof | |
| CN102533624B (en) | Microorganism bacteria mixture and application thereof in promoting production of methane by dregs of traditional Chinese medicine by anaerobic fermentation | |
| CN100478437C (en) | Method of producing fuel alcohol by kitchen garbage fermentation | |
| CN1944658B (en) | Method for producing cellulose alcohol using corncob processing leftover by fermenting | |
| CN100342022C (en) | Method for improving alcohol yield fermented from starch material | |
| CN1896254A (en) | Production of alcohol from mixed bacterial population degradable fermented bastose substance | |
| CN103725624A (en) | Genetic recombinant saccharomyces cerevisiae capable of degrading and utilizing kitchen wastes | |
| US20140302566A1 (en) | Enhanced ethanol fermentation using biodigestate | |
| CN101705199B (en) | Methane-producing composite microbial inoculum and preparation method thereof | |
| CN101139577B (en) | Glucoamylase produced by fermentation of wine lees miscible liquid and method for producing alcohol by fermenting restaurant garbage with this glucoamylase | |
| CN103589647A (en) | Monascus purpureus YH-6 strain, application thereof and esterified monascus prepared from strain | |
| CN101760498A (en) | Method of co-fermenting kitchen waste with mixed bacteria for producing fuel ethanol | |
| CN106893682B (en) | Method for expanding culture of saccharomycetes by using liquefied mash and application of saccharomycetes and method for fermenting ethanol | |
| CN104845889A (en) | Preparation method of liquid or solid rumen functional microbial agent and application thereof | |
| CN101709309B (en) | Method for combined fermentation of ethanol and xylitol | |
| CN105347481A (en) | Method for purifying low-carbon-nitrogen-ratio domestic sewage by biological fermentation coupling artificial wetland system | |
| CN103555638B (en) | A kind of alcohol patience produces acetic acid microorganism composite bacteria and uses thereof | |
| CN100513562C (en) | Preparation method of hybrid hydrolase formulation for promoting methane yield from fermentation | |
| CN110452822B (en) | Composite wine brewing yeast for brewing wine by using vinasse fermentation and wine brewing method thereof | |
| CN108588135B (en) | Method for producing lactic acid by combined fermentation of kitchen waste and waste grease biodiesel byproduct crude glycerol | |
| CN110862887A (en) | Method for manufacturing strong aromatic white spirit pit mud | |
| HUE027235T2 (en) | Method for fermenting biomass | |
| CN1114695C (en) | Method for producing alcohol by using plant fiber as raw material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100630 Termination date: 20130824 |