CN101130761A - Generation bacterium of ring dextrin glucosyl transferase - Google Patents
Generation bacterium of ring dextrin glucosyl transferase Download PDFInfo
- Publication number
- CN101130761A CN101130761A CNA2007100660321A CN200710066032A CN101130761A CN 101130761 A CN101130761 A CN 101130761A CN A2007100660321 A CNA2007100660321 A CN A2007100660321A CN 200710066032 A CN200710066032 A CN 200710066032A CN 101130761 A CN101130761 A CN 101130761A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- enzyme
- glucosyl transferase
- ring dextrin
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a manufactured bacterium of annular dextrin glucosyltransferase, which is characterized by the following: obtaining the separated strain LZ-10 as Bacillus sp with preservation number at CGMCC No. 2096; displaying the fittest reacting pH value of annular dextrin glucosyltransferase at 9. 0 and the fittest reacting temperature at 65 deg. c with good thermal stability; insulating at 60-65 deg. c over 30min to keep residual enzymatic activity more than 95%; insulating with pH value at 8. 5-9. 5 for 30 min to keep the residual enzymatic activity more than 90%. The enzyme is compatible with thermostability and alkaliresistant, which is fit for enzymatically industrial manufacturing course of annular dextrin.
Description
Technical field
The present invention relates to microbial technology field, specifically a kind of generation bacterium of ring dextrin glucosyl transferase.
Background technology
Ring dextrin glucosyl transferase (Cyclodextrin Glucanotransferase EC 2.4.1.19 is called for short CGTase) is the enzyme of catalysis starch synthesis of cyclic dextrin.Cyclodextrine (cyclodextrin, be called for short CD) be with α-1 by the D-glucopyranosyl, 4 glucoside bonds connect into the annular oligose that ring constitutes, and the difference according to forming the glucosyl group number is called α, β, γ-Huan Hujing (the glucosyl group number is respectively 6,7,8) respectively.As one of bionic field, act on the cyclodextrine that starch is made with ring dextrin glucosyl transferase, cylinder-like structure with hollow, inner hydrophobic, outside hydrophilic, can cover material complexing becoming molecule inclusion compound with the bag in the molecule gap, can connect gas, halogen, dyestuff, spices, agricultural chemicals, food etc. by bag, be described as " molecular capsule " and " crown compound ", thereby have a wide range of applications in fields such as food, medicine, chemistry, agricultural, analytical chemistry and environmental protection.The whole world consumed 6000 tons of cyclodextrin altogether in 1998, was worth to reach 100,000,000 dollars, and cyclodextrin market reached 2.5 hundred million dollars in 2002.
Though cyclodextrine also can obtain by the method for chemosynthesis, the beta-cyclodextrin yield of chemical synthesis process is extremely low, only is suitable for the purpose of scientific research.Therefore, existing suitability for industrialized production all is to adopt enzyme process synthesis of cyclic dextrin, and promptly under the cyclomaltodextrin glucanotransferase katalysis, converted starch reaches relevant matrix synthesis of cyclic dextrin.
Fundamental research to ring dextrin glucosyl transferase just began as far back as the thirties in last century, the various data of having reported in succession since 1973 show, the various bacteria of bacillus can both produce CGTase, bacillus macerans (B.macerans 1904) for example, bacillus megaterium (B.megaterium 1972), Bacillus circulans (B.circulans 1973), bacstearothermophilus (B.stearothermophilus 1973), basophilia genus bacillus (Alkalophilic Bacillus nineteen eighty-two) and klebsiella pneumoniae (Klebsiella pueumoniae 1977) etc., wherein with the enzyme heat stability height of bacstearothermophilus, the bacillus megaterium yield of enzyme is big.In addition, it is reported that the light family of China woods finds that asporulate alkaliphile (1985) also can produce CGTase, is the distinctive bacterial classification of China.Now verified, the bacterium that can produce CGTase mainly can be divided into two big classes: a class is to generate α-CD, and what generally use is bacillus macerans, bacstearothermophilus and klebsiella pneumoniae more; Another kind of to generate β-CD, general many with bacillus megaterium, Bacillus circulans and Alkaliphilic bacillus, its zymologic property is difference to some extent.
Production and applied research to cyclodextrine starts from the seventies in last century in the world, Japan is the country that takes the lead in realizing the cyclodextrine suitability for industrialized production in the world, the main manufacturer of cyclodextrin has French Cerestar, German Wacker Biochem and Japanese Ensuiko Sugar etc. on the world market, and Japan is being in rank first aspect the production of cyclodextrin and the applied research.China began exploratory study is carried out in the production and the application of cyclodextrine the end of the seventies in last century and the beginning of the eighties, because the fermentation condition of basophilia genus bacillus is comparatively extensive, be easy to control and operation, production cost is relatively low, therefore China is industrial substantially all adopts the basophilia genus bacillus to produce cyclodextrine, but generally the yielding poorly of China's cyclodextrine, price is more expensive and purity is not high, therefore the research to cyclodextrin production has great significance.
This shows, ring dextrin glucosyl transferase is the key enzyme of suitability for industrialized production cyclodextrine, yet in using the process of CGTase by starch industry production cyclodextrine, because high temperature (85 ℃～90 ℃) and highly basic (pH 8.5) are indispensable reaction conditionss, therefore heat-resisting, alkali proof ring dextrin glucosyl transferase just has commercialization value.
Summary of the invention
The generation bacterium that the purpose of this invention is to provide a kind of ring dextrin glucosyl transferase, the ring dextrin glucosyl transferase enzymic activity that this bacterium produces is good, very stable, has good thermostability.
The present invention utilizes the abundant advantage of Yunnan Province's Microbial resources, around the high wheat field of starch content, starch factory, grain depot etc., screen bacillus (Bacillus sp) bacterial strain that ring dextrin glucosyl transferase is produced in a strain, from name LZ-10, deposit number is CGMCC No.2096, preservation date is on June 22nd, 2007, depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC).The optimal reaction pH of its thick enzyme is 9.0, and optimal reactive temperature is 65 ℃, and better heat stability is incubated the remnant enzyme activity that still kept more than 95% in 30 minutes down at 60 ℃-65 ℃, and insulation still keeps the remnant enzyme activity more than 90% in the time of 30 minutes under the pH8.5-9.5 condition.
The preparation method of this bacterial strain is:
1) separation of bacterial strain and screening: around the wheat field of rich in starch, starch factory, grain depot etc., gather soil sample, take by weighing in soil sample 5g to the 45ml physiological saline, add granulated glass sphere and fully shake and be placed in the boiling water bath 5 minutes, left standstill 1 hour.Soil sampling supernatant liquor 100 μ l do gradient dilution in test tube, get suitable extent of dilution coating screening flat board, cultivate 48hr for 37 ℃ in electro-heating standing-temperature cultivator.(1,3,5 day, difference) observes the size that bacterium colony formed and formed the color changeable transparent circle after cultivating a couple of days, and picking has yellow transparent circle person, the separation and purification of ruling.To carry out shake flask fermentation in the access of the bacterial strain behind the purifying liquid fermentation medium, by the bacterial strain LZ-10 of the final separation screening of enzyme activity determination to product ring dextrin glucosyl transferase enzyme height alive and good stability.
2) used screening culture medium: Zulkovsky starch 1%, anhydrous sodium carbonate 1%, peptone 1%, yeast extract paste 0.5%, K
2HPO
4.3H
2O 0.13%, MgSO
4.7H
2O 0.02%, phenolphthalein 0.03%, agar 2% (pH9.0).
3) used fermention medium: W-Gum 1%, yeast extract paste 0.1%, Na
2CO
30.5%, K
2HPO
4.3H
2O0.13%, MgSO
4.7H
2O 0.02%.
The preliminary evaluation of bacterial strain:
(1) evaluation of measuring according to form, cultural characteristic and Physiology and biochemistry, bacterial strain LZ-10 is the Gram-positive genus bacillus, the gemma oval.Can grow in 10～70 ℃ of temperature ranges, optimum growth temperature is 28～37 ℃, and growth pH is 7.0～10.0, and the suitableeest growth pH is 8.0～9.0.The catalase reaction positive, aerobic growth, the V.P. reacting positive, hydrolyzed starch and gelatin do not utilize Citrate trianion, do not reduce nitrate, do not form indoles and otan etc.
(2) according to the comparison of 16S rDNA sequence similarity, 16S rDNA and the Bacillussp.DSM 8714 of LZ-10 have 99% homology, with Bacillus patagoniensis PAT 05
T99% homology is arranged, 97% homology is arranged with Bacillus sp.WW3-SN6.The 16S rDNA gene of bacterial strain is (the GenBank number of landing is DQ400103):＞LZ-10
CCGTTGCCAGTATGGCTACCATCCTTGCATTCCGAAGTCGAGCGGACAGAAGGGAGCTTGCTCCCG
GAAGTCAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCCCTTAGACTGGGATAACTTCGGG
AAACCGAAGCTAATACCGGATAACACTTTTTCCTACCTGGGAGAAAGTTAAAAGATGGCCTTTGTG
CTATCACTAGGGGATGGGCCCGCGGCGCATTAGCTAGTTGGTAAGGTAATGGCTTACCAAGGCGAC
GATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAAACACGGCCCAGACTCCTACG
GGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAATGCCGCGTGAGTGAG
GAAGGCCTTCGGGTCGTAAAGCTCTGTTGTGAGGGAAGAACAAGTATCGGTTGAATAAGCCGGTAC
CTTGACGGTACCTCACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTG
GCAAGCGTTGTCCGGAATTATTGGGCCGTAAAGCGCGCGCAGGCGGCTTCTTAAAGTCTGATGTGA
AATCTCGGGGCTCACCCCCGAGCGGCCATTGGAAACTGGGAAGCTTGAGTGCAGAAGAGGAGAGTG
GAATTCCACGTGTAGCGGTGAAATGCGTAAGATATGTGGAGGAACACCAGTGGGGAAGGCGACTCT
CTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT
CCACGCCGTAAACGATGAGTGCTAGGTGTTAGGGGTTTCGATGCCCGTAGTGCCGAAGTAAACACA
TTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGACCCGCA
CAAGCAGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTT
TGACCACTCTGGAGACAGAGCTTCCCCTTCGGGGGCAAAGTGACAGGTGGTGCATGGTTGTCGTCA
GGTCGTGTCGTGAGATGTTGGGTTAAGTGGGGGAAGGAGGGGAAGGCTTGATCTTAGTTGCCAGCA
TTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAAATC
ATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGTTGCGAAGCCG
CGAGGTGAAGCCAATCCCATAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGA
AGCCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACA
CCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCG
CCTAAGGTGGGACAAATGATTGGGGTGAAGTCGTAACAAGGTAGCCT
In conjunction with the distinctive feature of aspects such as the colony characteristics of bacterial strain, physio-biochemical characteristics, 16S rDNA homology analysis, preliminary evaluation bacterial strain LZ-10 is bacillus (Bacillus sp).
The fermentation of bacterial strain:
The seed culture fluid access is equipped with in the triangular flask of 30ml fermention medium, places the speed governing of rotary type constant temperature to shake a bottle cabinet and carry out aerobic fermentation, rotating speed is 200rpm/min, and 37 ℃ fermented 4 days.Fermented liquid is centrifugal, gets supernatant liquor (containing ring dextrin glucosyl transferase), places 4 ℃ of preservations.Used fermention medium: W-Gum 1%, yeast extract paste 0.1%, Na
2CO
30.5%, K
2HPO
4.3H
2O 0.13%, MgSO
4.7H
2O 0.02%.
Enzyme activity determination method and thick enzyme zymologic property:
(1) the enzyme activity determination method (see: H.J.Fuwa.A new method for microdetermination ofamylase activity by the use of amylase as the substrate[J] .Biochemistry, 1954 (41): 583-603)
1. principle: show blueness after yam starch and the iodine solution effect, the colour-change that makes iodine solution cause after starch is hydrolyzed into β-CD by β-CGTase dies down.
2. determination step: get the suitably enzyme liquid of dilution of 0.01ml, the Gly-NaOH damping fluid (pH 9.0) that adds 0.2ml 0.2M, add dissolved 0.2% potato starch solution 0.2ml again, 40 ℃ were reacted 10 minutes, add 0.5M Glacial acetic acid 0.5ml termination reaction, add the colour developing of 3ml 0.005% iodine solution, do blank with the reaction solution that does not add starch solution, with not enzyme-added liquid is contrast, measures absorbancy (OD value) at 700nm.
3. the enzyme definition of living: unit of activity is defined as the required enzyme amount of blue value decline 10%.
4. formula is calculated in enzyme work:
Wherein, a is control group OD value, and b is sample OD value.
(2) thick enzyme zymologic property (seeing accompanying drawing)
The present invention separates the strain excellent LZ-10 that obtains producing ring dextrin glucosyl transferase, its optimal reaction pH that produces the thick enzyme of ring dextrin glucosyl transferase is 9.0, optimal reactive temperature is 65 ℃, better heat stability, be incubated the remnant enzyme activity that still kept more than 95% in 30 minutes down at 60 ℃-65 ℃, insulation still keeps the remnant enzyme activity more than 90% in the time of 30 minutes under the pH8.5-9.5 condition.Compare with the ring dextrin glucosyl transferase of having reported, this enzyme has heat-resisting and alkaline-resisting characteristic concurrently, is fit to be applied to the enzyme process commercial process of cyclodextrine.
Description of drawings
Fig. 1 is the optimum temperuture curve during thick enzyme zymologic property of the present invention detects.
Fig. 2 is the optimal pH curve during thick enzyme zymologic property of the present invention detects.
Fig. 3 is that temperature during thick enzyme zymologic property of the present invention detects is to the influence of enzyme stability.
Fig. 4 is that pH during thick enzyme zymologic property of the present invention detects is to the influence of enzyme stability.
The microbial preservation date of the present invention is on June 22nd, 2007, and depositary institution is called for short CGMCC, the address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, deposit number: CGMCC No.2096, classification name: bacillus sp..
Embodiment
Embodiment:
1, the separation of bacterial strain and screening:
Around the wheat field of rich in starch, starch factory, grain depot etc., gather soil sample, take by weighing in soil sample 5g to the 45ml physiological saline, add granulated glass sphere and fully shake and be placed in the boiling water bath 5 minutes, left standstill 1 hour; Soil sampling supernatant liquor 100 μ l do gradient dilution in test tube, get suitable extent of dilution coating screening flat board, cultivate 48hr for 37 ℃ in electro-heating standing-temperature cultivator.Cultivate the size that (1,3,5 day respectively) observes bacterium colony formation and formation color changeable transparent circle after 5 days, picking has yellow transparent circle person, the line separation and purification; To carry out shake flask fermentation in the access of the bacterial strain behind the purifying liquid fermentation medium, by the bacterial strain LZ-10 of the final separation screening of enzyme activity determination to product ring dextrin glucosyl transferase enzyme height alive and good stability.
Used screening culture medium: Zulkovsky starch 1%, anhydrous sodium carbonate 1%, peptone 1%, yeast extract paste 0.5%, K
2HPO
4.3H
2O 0.13%, MgSO
4.7H
2O 0.02%, phenolphthalein 0.03%, agar 2% (pH9.0).
Used fermention medium: W-Gum 1%, yeast extract paste 0.1%, Na
2CO
30.5%, K
2HPO
4.3H
2O0.13%, MgSO
4.7H
2O 0.02%.
2, the preliminary evaluation of bacterial strain:
According to the evaluation that form, cultural characteristic and Physiology and biochemistry are measured, bacterial strain LZ-10 is the Gram-positive genus bacillus, the gemma oval.Can grow in 10～70 ℃ of temperature ranges, optimum growth temperature is 28～37 ℃, and growth pH is 7.0～10.0, and the suitableeest growth pH is 8.0～9.0.The catalase reaction positive, aerobic growth, the V.P. reacting positive, hydrolyzed starch and gelatin do not utilize Citrate trianion, do not reduce nitrate, do not form indoles and otan etc.
According to the comparison of 16S rDNA sequence similarity, 16S rDNA and the Bacillus sp.DSM8714 of LZ-10 have 99% homology, with Bacillus patagoniensis PAT 05
T99% homology is arranged, 97% homology is arranged with Bacillus sp.WW3-SN6.Bacterial strain 16S rDNA is DQ400103 in the number of landing of GenBank.In conjunction with the distinctive feature of aspects such as the colony characteristics of bacterial strain, physio-biochemical characteristics, 16S rDNA homology analysis, preliminary evaluation bacterial strain LZ-10 is bacillus (Bacillus sp).
3, the fermentation of bacterial strain:
The seed culture fluid access is equipped with in the triangular flask of 30ml fermention medium, places the speed governing of rotary type constant temperature to shake a bottle cabinet and carry out aerobic fermentation, rotating speed is 200rpm/min, and 37 ℃ fermented 4 days.Fermented liquid is centrifugal, gets supernatant liquor, places 4 ℃ of preservations.Used fermention medium: W-Gum 1%, yeast extract paste 0.1%, Na
2CO
30.5%, K
2HPO
4.3H
2O 0.13%, MgSO
4.7H
2O 0.02%.
CGTase patent _ ST25.txt
SEQUENCE?LISTING
＜110〉Yunnan Normal University
＜120〉generation bacterium of a kind of ring dextrin glucosyl transferase and preparation method thereof
<130>12
<160>1
<170>PatentIn?version?3.4
<210>1
<211>1499
<212>DNA
<213>Bacillus?sp.
<220>
<221>rRNA
<222>(1)..(1499)
<400>1
ccgttgccag?tatggctacc?atccttgcat?tccgaagtcg?agcggacaga?agggagcttg 60
ctcccggaag?tcagcggcgg?acgggtgagt?aacacgtggg?caacctgccc?cttagactgg 120
gataacttcg?ggaaaccgaa?gctaataccg?gataacactt?tttcctacct?gggagaaagt 180
taaaagatgg?cctttgtgct?atcactaggg?gatgggcccg?cggcgcatta?gctagttggt 240
aaggtaatgg?cttaccaagg?cgacgatgcg?tagccgacct?gagagggtga?tcggccacac 300
tgggactgaa?acacggccca?gactcctacg?ggaggcagca?gtagggaatc?ttccgcaatg 360
gacgaaagtc?tgacggagca?atgccgcgtg?agtgaggaag?gccttcgggt?cgtaaagctc 420
tgttgtgagg?gaagaacaag?tatcggttga?ataagccggt?accttgacgg?tacctcacca 480
gaaagccacg?gctaactacg?tgccagcagc?cgcggtaata?cgtaggtggc?aagcgttgtc 540
cggaattatt?gggccgtaaa?gcgcgcgcag?gcggcttctt?aaagtctgat?gtgaaatctc 600
ggggctcacc?cccgagcggc?cattggaaac?tgggaagctt?gagtgcagaa?gaggagagtg 660
gaattccacg?tgtagcggtg?aaatgcgtaa?gatatgtgga?ggaacaccag?tggggaaggc 720
gactctctgg?tctgtaactg?acgctgaggc?gcgaaagcgt?ggggagcaaa?caggattaga 780
taccctggta?gtccacgccg?taaacgatga?gtgctaggtg?ttaggggttt?cgatgcccgt 840
agtgccgaag?taaacacatt?aagcactccg?cctggggagt?acgaccgcaa?ggttgaaact 900
caaaggaatt?gacggggacc?cgcacaagca?gtggagcatg?tggtttaatt?cgaagcaacg 960
cgaagaacct?taccaggtct?tgacatcctt?tgaccactct?ggagacagag?cttccccttc 1020
gggggcaaag?tgacaggtgg?tgcatggttg?tcgtcagctc?gtgtcgtgag?atgttgggtt 1080
aagtcccgca?acgagcgcaa?cccttgatct?tagttgccag?catttagttg?ggcactctaa 1140
ggtgactgcc?ggtgacaaac?cggaggaagg?tggggacgac?gtcaaatcat?catgcccctt 1200
atgacctggg?ctacacacgt?gctacaatgg?atggtacaaa?gggttgcgaa?gccgcgaggt 1260
gaagccaatc?ccataaagcc?attctcagtt?cggattgtag?gctgcaactc?gcctacatga 1320
agccggaatt?gctagtaatc?gcggatcagc?atgccgcggt?gaatacgttc?ccgggtcttg 1380
tacacaccgc?ccgtcacacc?acgagagttt?gtaacacccg?aagtcggtga?ggtaaccttt 1440
tggagccagc?cgcctaaggt?gggacaaatg?attggggtga?agtcgtaaca?aggtagcct 1499
Claims (2)
1. the generation bacterium of a ring dextrin glucosyl transferase is bacillus (Bacillus sp) that its deposit number is CGMCC No.2096.
2. ring dextrin glucosyl transferase according to claim 1 produces bacterium, it is characterized in that the 16SrDNA gene of this bacterial strain LZ-10 is:
CCGTTGCCAGTATGGCTACCATCCTTGCATTCCGAAGTCGAGCGGACAGAAGGGAGCTTGCTCCCG
GAAGTCAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCCCTTAGACTGGGATAACTTCGGG
AAACCGAAGCTAATACCGGATAACACTTTTTCCTACCTGGGAGAAAGTTAAAAGATGGCCTTTGTG
CTATCACTAGGGGATGGGCCCGCGGCGCATTAGCTAGTTGGTAAGGTAATGGCTTACCAAGGCGAC
GATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAAACACGGCCCAGACTCCTACG
GGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAATGCCGCGTGAGTGAG
GAAGGCCTTCGGGTCGTAAAGCTCTGTTGTGAGGGAAGAACAAGTATCGGTTGAATAAGCCGGTAC
CTTGACGGTACCTCACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTG
GCAAGCGTTGTCCGGAATTATTGGGCCGTAAAGCGCGCGCAGGCGGCTTCTTAAAGTCTGATGTGA
AATCTCGGGGCTCACCCCCGAGCGGCCATTGGAAACTGGGAAGCTTGAGTGCAGAAGAGGAGAGTG
GAATTCCACGTGTAGCGGTGAAATGCGTAAGATATGTGGAGGAACACCAGTGGGGAAGGCGACTCT
CTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGT
CCACGCCGTAAACGATGAGTGCTAGGTGTTAGGGGTTTCGATGCCCGTAGTGCCGAAGTAAACACA
TTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGACCCGCA
CAAGCAGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTT
TGACCACTCTGGAGACAGAGCTTCCCCTTCGGGGGCAAAGTGACAGGTGGTGCATGGTTGTCGTCA
GCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA
TTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAAATC
ATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGTTGCGAAGCCG
CGAGGTGAAGCCAATCCCATAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGA
AGCCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACA
CCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCG
CCTAAGGTGGGACAAATGATTGGGGTGAAGTCGTAACAAGGTAGCCT
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100660321A CN101130761A (en) | 2007-07-13 | 2007-07-13 | Generation bacterium of ring dextrin glucosyl transferase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100660321A CN101130761A (en) | 2007-07-13 | 2007-07-13 | Generation bacterium of ring dextrin glucosyl transferase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101130761A true CN101130761A (en) | 2008-02-27 |
Family
ID=39128167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100660321A Pending CN101130761A (en) | 2007-07-13 | 2007-07-13 | Generation bacterium of ring dextrin glucosyl transferase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101130761A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104130955A (en) * | 2013-05-03 | 2014-11-05 | 中国水产科学研究院黄海水产研究所 | Marine microorganism strain cd82 capable of producing cyclodextrine glucosyltransferase |
| CN105154352A (en) * | 2015-07-23 | 2015-12-16 | 中国水产科学研究院黄海水产研究所 | Marine microorganism strain Y112 and alpha-cyclodextrin glycosyltransferase produced by strain |
| CN111820219A (en) * | 2020-07-29 | 2020-10-27 | 山东滨州智源生物科技有限公司 | Application of hydroxypropyl-beta-cyclodextrin in preparation of antibacterial material, food package and preparation method of food package |
| CN111893126A (en) * | 2020-07-01 | 2020-11-06 | 深圳润康生态环境股份有限公司 | Alkaline protease gene, alkaline protease, preparation method and application thereof |
| WO2023000618A1 (en) * | 2021-07-22 | 2023-01-26 | 江南大学 | Bacillus xiaoxiensis and application thereof |
-
2007
- 2007-07-13 CN CNA2007100660321A patent/CN101130761A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104130955A (en) * | 2013-05-03 | 2014-11-05 | 中国水产科学研究院黄海水产研究所 | Marine microorganism strain cd82 capable of producing cyclodextrine glucosyltransferase |
| CN104130955B (en) * | 2013-05-03 | 2017-03-22 | 中国水产科学研究院黄海水产研究所 | Marine microorganism strain cd82 capable of producing cyclodextrine glucosyltransferase |
| CN105154352A (en) * | 2015-07-23 | 2015-12-16 | 中国水产科学研究院黄海水产研究所 | Marine microorganism strain Y112 and alpha-cyclodextrin glycosyltransferase produced by strain |
| CN105154352B (en) * | 2015-07-23 | 2018-07-13 | 中国水产科学研究院黄海水产研究所 | A kind of marine microorganism bacterial strain Y112 and its production alpha-cyclodextrin glucosyl transferase |
| CN111893126A (en) * | 2020-07-01 | 2020-11-06 | 深圳润康生态环境股份有限公司 | Alkaline protease gene, alkaline protease, preparation method and application thereof |
| CN111820219A (en) * | 2020-07-29 | 2020-10-27 | 山东滨州智源生物科技有限公司 | Application of hydroxypropyl-beta-cyclodextrin in preparation of antibacterial material, food package and preparation method of food package |
| WO2023000618A1 (en) * | 2021-07-22 | 2023-01-26 | 江南大学 | Bacillus xiaoxiensis and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chi et al. | Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain | |
| Ge et al. | Improvement of l-lactic acid production from Jerusalem artichoke tubers by mixed culture of Aspergillus niger and Lactobacillus sp. | |
| WO2001090335A1 (en) | Novel cyclodextrin glucanotransferase, process for producing the same and process for producing cyclodextrin by using this enzyme | |
| Shamala et al. | Preliminary studies on the production of high and low viscosity dextran by Leuconostoc spp. | |
| CN101130761A (en) | Generation bacterium of ring dextrin glucosyl transferase | |
| CN103667102A (en) | Bacterial strain for cyclodextrin glycosyltransferase production and application thereof | |
| CN100451106C (en) | Sphingomonaspaucimobilis of high-yield gellan gum and use therefor | |
| Kumar et al. | Optimization of process parameters for the production of inulinase from a newly isolated Aspergillus niger AUP19 | |
| Singh et al. | Microbial inulinases and pullulanases in the food industry | |
| CN101157904B (en) | Producing strain for beta-dextranase | |
| Kataoka et al. | Isolation and characterization of an active mannanase‐producing anaerobic bacterium, Clostridium tertiumKT‐5A, from lotus soil | |
| Chaudhary et al. | Thermophilic actinomycetes from hot water spring capable of producing enzymes of industrial importance | |
| US4737459A (en) | Regulation and enhancement of enzyme production | |
| CN103981125B (en) | The Geobacillus stearothermophilus bacterial strain of cyclodextrin glycosyltransferase is produced in one strain | |
| CN100374555C (en) | Method for preparing beta-cyclodextrin by yeast | |
| CN107760609B (en) | A kind of suitable macromolecule of production under alkaline condition, the bacterial strain of non-pigment pulullan polysaccharide and its application | |
| RU2001103C1 (en) | Bacterial strain m-bacillus licheniformis as a producer of thermostable amlolytic enzymes | |
| CN103205406B (en) | A kind of method utilizing subtilis 6-7 to produce heat-resisting beta-amylase | |
| CN106520634B (en) | A kind of rhizobium and its application in trehalose preparation | |
| CN110093297A (en) | Nitrate reduction bacterium MCDA3-3 and its method for producing chitin deacetylase | |
| KR20020084677A (en) | Microorganism producing fructosyl transferase and method for producing fructooligosaccharides and neofructooligosaccharides using the same | |
| Alves-Prado et al. | Evaluation of solid and submerged fermentations for the production of cyclodextrin glycosyltransferase by Paenibacillus campinasensis H69-3 and characterization of crude enzyme | |
| CN109868245A (en) | A kind of bacillus subtilis BSLN-08 and its cultural method and application | |
| CN104031846A (en) | Aspergillus oryzae bacterial strain and application thereof | |
| CN109971688B (en) | Low-impurity high-yield gellan gum producing strain and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080227 |