CN101120099B - 使用由互补于靶mRNA的核苷酸序列组成的siRNA抑制靶mRNA表达的方法 - Google Patents

使用由互补于靶mRNA的核苷酸序列组成的siRNA抑制靶mRNA表达的方法 Download PDF

Info

Publication number
CN101120099B
CN101120099B CN2005800478328A CN200580047832A CN101120099B CN 101120099 B CN101120099 B CN 101120099B CN 2005800478328 A CN2005800478328 A CN 2005800478328A CN 200580047832 A CN200580047832 A CN 200580047832A CN 101120099 B CN101120099 B CN 101120099B
Authority
CN
China
Prior art keywords
sirna
section
dsrna
distribute
factor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2005800478328A
Other languages
English (en)
Chinese (zh)
Other versions
CN101120099A (zh
Inventor
崔泳哲
朴翰浯
郑素林
金永柱
金尚洙
朴城敏
金相喆
尹圭晚
崔庆玉
姜孝晋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bioneer Corp
Original Assignee
Bioneer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioneer Corp filed Critical Bioneer Corp
Publication of CN101120099A publication Critical patent/CN101120099A/zh
Application granted granted Critical
Publication of CN101120099B publication Critical patent/CN101120099B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/50—Mutagenesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2005800478328A 2004-12-08 2005-12-08 使用由互补于靶mRNA的核苷酸序列组成的siRNA抑制靶mRNA表达的方法 Expired - Fee Related CN101120099B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20040103283 2004-12-08
KR1020040103283 2004-12-08
KR10-2004-0103283 2004-12-08
PCT/KR2005/004207 WO2006062369A1 (en) 2004-12-08 2005-12-08 Method of inhibiting expression of target mrna using sirna consisting of nucleotide sequence complementary to said target mrna

Publications (2)

Publication Number Publication Date
CN101120099A CN101120099A (zh) 2008-02-06
CN101120099B true CN101120099B (zh) 2010-12-15

Family

ID=36578152

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2005800478328A Expired - Fee Related CN101120099B (zh) 2004-12-08 2005-12-08 使用由互补于靶mRNA的核苷酸序列组成的siRNA抑制靶mRNA表达的方法

Country Status (6)

Country Link
US (1) US20090155904A1 (https=)
EP (1) EP1828415A4 (https=)
JP (1) JP4672021B2 (https=)
KR (1) KR101007346B1 (https=)
CN (1) CN101120099B (https=)
WO (1) WO2006062369A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2015014666A (es) 2013-04-17 2016-03-01 Pfizer Derivados de n-piperidin-3-ilbenzamida para tratar enfermedades cardiovasculares.
CN105176999A (zh) * 2015-08-13 2015-12-23 吉林大学 抑制survivin基因表达的双链siRNA、其应用及包含其的表达质粒及传递体
CN105063048A (zh) * 2015-08-13 2015-11-18 吉林大学 一种抑制Survivin基因表达的siRNA及其应用
CN112951322B (zh) * 2021-03-08 2023-09-26 深圳市新合生物医疗科技有限公司 一种基于网格搜索的规则权重分配siRNA设计方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1427008A (zh) * 2001-12-14 2003-07-02 殷冬生 设计与选择天然的siRNA作为基因药物的方法及药物配方

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040002083A1 (en) * 2002-01-29 2004-01-01 Ye Ding Statistical algorithms for folding and target accessibility prediction and design of nucleic acids
EP2305812A3 (en) 2002-11-14 2012-06-06 Dharmacon, Inc. Fuctional and hyperfunctional sirna

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1427008A (zh) * 2001-12-14 2003-07-02 殷冬生 设计与选择天然的siRNA作为基因药物的方法及药物配方

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Ola Snove Jr. et al.Designing effective siRNAs with off-target control.Biochemical and Biophysical Research Communications325.2004,325769-773. *
Weiping Chen et al.A computer program for the optimal design of short interferingRNA(siRNA) for gene silencing.Proceedings of the 17th IEEE symposium on Computer-Based Medical Systems.2004,335-342. *

Also Published As

Publication number Publication date
EP1828415A4 (en) 2009-07-01
KR101007346B1 (ko) 2011-01-13
WO2006062369A1 (en) 2006-06-15
JP4672021B2 (ja) 2011-04-20
CN101120099A (zh) 2008-02-06
EP1828415A1 (en) 2007-09-05
KR20070094601A (ko) 2007-09-20
JP2008522613A (ja) 2008-07-03
US20090155904A1 (en) 2009-06-18

Similar Documents

Publication Publication Date Title
Shabalina et al. Computational models with thermodynamic and composition features improve siRNA design
SaeTrom et al. Weighted sequence motifs as an improved seeding step in microRNA target prediction algorithms
CN1926551B (zh) 用于基因沉默的siRNA的设计方法
Zhao et al. Methods of MicroRNA promoter prediction and transcription factor mediated regulatory network
Galatenko et al. Comprehensive network of miRNA-induced intergenic interactions and a biological role of its core in cancer
CN107679367B (zh) 一种基于网络节点关联度的共调控网络功能模块识别方法及系统
CN1890370A (zh) RNAi潜能的预测方法
CN105808976B (zh) 一种基于推荐模型的miRNA靶基因预测方法
CN106874704B (zh) 一种基于线性模型的基因共调控网络中关键调控子识别方法
Chiu et al. Parameter optimization for constructing competing endogenous RNA regulatory network in glioblastoma multiforme and other cancers
Mysara et al. MysiRNA-designer: a workflow for efficient siRNA design
Li et al. The architecture of the gene regulatory networks of different tissues
CN108681660A (zh) 一种基于关联规则挖掘的非编码rna与疾病关系预测方法
Zhu et al. Evolutionary relationships between miRNA genes and their activity
Zhang et al. Time to infer miRNA sponge modules
Hirose et al. Precise mapping and dynamics of tRNA-derived fragments (tRFs) in the development of Triops cancriformis (tadpole shrimp)
Xiao et al. Identification of microRNA precursors based on random forest with network-level representation method of stem-loop structure
CN101120099B (zh) 使用由互补于靶mRNA的核苷酸序列组成的siRNA抑制靶mRNA表达的方法
JP2006525811A (ja) Ｒｎａ干渉の方法と組成物
Xie et al. Identification of key genes and pathways associated with resting mast cells in meningioma
Liu et al. The effect of regions flanking target site on siRNA potency
Ulbricht et al. One hundred million adenosine‐to‐inosine RNA editing sites: Hearing through the noise
Shi et al. The partitioned LASSO-patternsearch algorithm with application to gene expression data
Liu et al. Reconsideration of in silico siRNA design from a perspective of heterogeneous data integration: problems and solutions
US20120072123A1 (en) Methods of Predicting The Probability of Modulation of Transcript Levels By RNAI Compounds

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20101215

Termination date: 20171208

CF01 Termination of patent right due to non-payment of annual fee