CN101109736B - Method for detecting fingerprint pattern of tuckahoe fat-soluble component - Google Patents
Method for detecting fingerprint pattern of tuckahoe fat-soluble component Download PDFInfo
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Abstract
The invention provides an inspection method for fingerprint atlas of grease soluble components in poria cocos. HPLC-UV chromatography is employed to check the detection wavelength, chromatograph column, flowing phase, column flow rate, column temperature, chromatograph condition time, blank flow phase, blank solvent and the method for preparing the test piece, etc. for the fingerprint atlas; the relative retain time, relative percentage area, relative percentage height, the similar coefficient of spectrum of the common peak as intrinsic parameters. The invention is characterized in that 210 nm is taken as the detection wavelength for the fingerprint atlas of grease soluble components in poria cocos. Different medical parts of poria cocos are different, the grease soluble components in poria cocos has the most information at the 210 nm wavelength. Selecting 210 nm as inspection wavelength makes the method simple, steady, accurate and highly repeatable.
Description
Technical field
Chinese crude drug finger-print Quality Control technology.
Background technology
Poria cocos is that wild, cultivation has kind concurrently, main product in Hubei, provinces such as Hunan, Anhui, Guizhou, Yunnan, Henan, Sichuan, Guangxi, Guangdong because place of production difference, its quality exists tangible difference.Luotian, Hubei is one of genuine place of production of Poria cocos, and it is best in quality that tradition is thought." under 2005 editions Poria cocos items of Chinese pharmacopoeia, the quality control of Poria cocos medicinal material is had only items such as proterties, discriminating and inspection, be difficult to control effectively the quality of Poria cocos medicinal material.
Tuckahoe fat-soluble component finger-print research principle is, Chemistry for Chinese Traditional Medicine finger-print (abbreviation finger-print) means chromatogram that can indicate main chemical compositions in this Chinese crude drug or the Chinese patent drug or the spectrum atlas that adopts certain analysis means to obtain, as the method for traditional Chinese medicine quality control.
At present domestic have a lot of researchists that the Poria cocos finger-print has been carried out deep research, for solid foundation has been established in traditional Chinese medicine fingerprint Study on Technology and application.For example, several professors expert such as, Xu Xiuying one quick by Yi Zhonghong, the Wang Long of University Of Chongqing's biomechanics and key lab of organizational project Ministry of Education associating Chongqing Zheng of traditional Chinese medicine research institute adopt the HPLC chromatography, selecting the relative retention time at each total peak, relative percentage area, relative percentage height, spectrum similarity coefficient, 3 purity quotients for use is characterization parameter, Poria cocos medicinal materials fingerprint Fuzzy Pattern Recognition is studied, and set up Poria cocos medicinal materials fingerprint mode standard.The result shows that its mode standard and sample have good similarity, can keep consistent with the morphology qualification result, and can analyze the Poria cocos medicinal materials fingerprint from qualitative, quantitative angle.Simultaneously domestic also have many researchists that the work of setting up of the triterpenes chemical constitution finger-print of Poria cocos is studied, and for example the Yang Ran of Zhengzhou University, Li JianJun, in the wrong riding the waves are studied the finger-print of the HPLC chromatogram of Poria cocos terpene with the several experts such as benevolence, An Dengkui that grasp mutually of analytic centre of China Medicine University.Multidigit expert professors such as the fourth hilllock of Institute of Analysis of people's associating China Medicine University such as the Wang Zhenzhong of Kangyuan Pharmaceutical Co., Ltd., Jiangsu Prov institute of materia medica, Xin Chenfeng, Sheng Longsheng point out nine chemical constitution chromatographic peaks wherein with the HPLC-MS coupling technique, and the clearer and more definite various product for Poria cocos medicinal material and Poria cocos provides the effective quality control means.
Beijing Poison ﹠ Medicine Inst. of Military Medicial Sciences Academy in can the river, Guo Jifen, Zhao Yimin, multidigit professors such as Zhang Yongxiang adopt HPLC/DAD/ESI-MS (high performance liquid chromatography-photodiode array detection-mass spectroscopy) through online detection, select the relative retention time at each total peak for use, relative percentage area, relative percentage height, the spectrum similarity coefficient is analyzed its uv-spectrogram and mass-spectrogram, and compare with document, 15 Poria cocos triterpene chemical constitutions have been identified, simultaneously also inferred remaining 8 triterpene chemical constitution, for Poria cocos triterpenes The Chemical Constituents has obtained important breakthrough.
Its principle: adopt HPLC/DAD/ESI-MS (high performance liquid chromatography-photodiode array detection-mass spectroscopy) through online detection, selecting the relative retention time at each total peak, relative percentage area, relative percentage height, spectrum similarity coefficient for use is characterization parameter, analyzes the finger-print that its uv-spectrogram and mass-spectrogram are set up.
Its step:
Select HYPERSIL C for use
18(4.6mm * 250mm, 5 μ m) chromatographic column is a filling agent with the octadecylsilane chemically bonded silica; With acetonitrile-water ((50: 50) (0 ~ 48min) → (60: 35) (48 ~ 68min) → (75: 25) (68 ~ 95min)) (phosphoric acid of 0.1%); Carry out high performance liquid chromatogram and separate, two kinds of online detections of detection method of two utmost point array pipes (DAD) and electrospray ionization mass spectrum (ESI-MS), flow velocity is 1.0ml/min, and the detection wavelength is 242nm, and column temperature is 25 ℃.
Existing method exists following shortcoming:
Find in test that 1, the different medicinal parts of Poria cocos medicinal material there are differences, very weak in the uv absorption at 242nm place as a lot of triterpenic acid constituents in Poria cocos piece and the fuling peel, having only just has stronger absorption endways.
2. this method does not use reference substance that the quality at fat-soluble fingerprint characteristic peak is estimated, so it is too much to read information in detection, makes detection time long, the process complexity.
3. this method feature peak number of being merely able to point out is 23, can't find out the composition in the tuckahoe fat-soluble more clearly, is unfavorable for high-precision evaluation Poria cocos quality.
Summary of the invention
The purpose of this invention is to provide a kind of tuckahoe fat-soluble component fingerprint atlas detection method, it have easy, stable, precision is high and the advantage of favorable reproducibility.
Technical scheme of the present invention is: a kind of fingerprint atlas detection method of tuckahoe fat-soluble component, adopt the HPLC-UV chromatography, simultaneously finger-print detection wavelength, chromatographic column, moving phase, column flow rate, column temperature, chromatographic condition time, blank moving phase, blank solvent, test sample preparation method are investigated, select the relative retention time at each total peak, relative percentage area, relative percentage height, spectrum similarity coefficient for use, be characterization parameter
It is characterized in that: be the detection wavelength of Poria cocos medicinal materials fat soluble component finger-print with 210nm.The different medicinal parts of Poria cocos medicinal material there are differences, and the Poria cocos medicinal materials fat soluble component selects for use 210nm to detect wavelength in the quantity of information maximum at 210nm wavelength place, make that this method is easy, stable, precision is high and favorable reproducibility.
The fingerprint atlas detection method of aforesaid tuckahoe fat-soluble component is characterized in that: with the octadecylsilane chemically bonded silica filling agent, and Waters Symmetry 110A C
18(250nm * 4.6mm, 5 μ m) chromatographic column; With the acetonitrile is mobile phase A; With 0.1% phosphoric acid is Mobile phase B; Carry out wash-out according to the gradient in the elution requirement; Flow velocity is 1.0ml/min, and column temperature is 30 ℃, and theoretical cam curve is by 3 beta-hydroxies-wool steroid-7,9 (11), and 24-triolefin-21 acid (dehydrotrametenolic acid) peak calculates, and should be not less than 3000;
Elution requirement is as follows:
Time (min) mobile phase A (%) Mobile phase B (%)
0~70 45→95 55→5
70~85 95 5。
With the acetonitrile is mobile phase A; With 0.1% phosphoric acid is that Mobile phase B and column temperature are 30 ℃, can obtain the more information amount; Select Waters Symmetry 110A C for use
18(250mm * 4.6mm, 5 μ m) chromatographic column finds that the post effect is higher, chromatographic peak separating effect the best.
The fingerprint atlas detection method of aforesaid tuckahoe fat-soluble component, it is characterized in that: point out out the Poria cocos retention time at No. 24 peaks, total peak of 54.13 minutes according to the method for the LC-MS of liquid chromatograph-mass spectrometer coupling: 3 beta-hydroxies-wool steroid-7,9 (11), 24-triolefin-21-acid is reference substance, Poria cocos to each place of production carries out " the chromatographic fingerprints of Chinese materia medica similarity evaluation system that finger-print detects and various places Poria cocos similarity is recommended according to pharmacopoeia commission, the 2004A version " software, estimate the similarity value between the sample of the different places of production.
The fingerprint atlas detection method of aforesaid tuckahoe fat-soluble component, it is characterized in that: determine 28 chromatographic peaks, utilizing the LC-MS technology to carry out Poria cocos medicinal materials fat soluble component finger-print detects, make the finger-print composition of foundation many, clearer and more definite, method is science, reliable more, and 28 chromatographic peaks are:
Useful effect
The present invention utilizes HPLC-UV method (high performance liquid chromatograph-UV-detector) to set up the fingerprint atlas detection method of Poria cocos medicinal materials fat soluble component, and carried out projects such as system suitability, test sample preparation method, methodology checking and investigated, the result shows with existing method and compares, this method is easy, accurate, favorable reproducibility, and utilizing LC-MS (liquid chromatograph-mass spectrometer coupling) technology that part chromatographic peak in the Poria cocos medicinal materials fat soluble component finger-print is identified, the finger-print that makes foundation is science, reliable more.
Description of drawings
Fig. 1 adopts Poria cocos medicinal materials fat soluble component finger-print common pattern of the present invention.
Fig. 2, the three-dimensional plot of Poria cocos medicinal materials fat soluble component finger-print during 210nm.
Fig. 3, the finger-print of Poria cocos medicinal materials fat soluble component under the different wave length condition.
Fig. 4,17 parts of Poria cocos medicinal materials fat soluble component finger-prints in all parts of the country.
Fig. 5, the contrast of tuckahoe fat-soluble finger-print in all parts of the country.
Fig. 6, representative finger-print comparison diagram of a Poria cocos medicinal material and fuling peel medicinal material and enlarged drawing.Among the figure, the L-fuling peel; The M-Poria cocos is individual.
Concrete embodiment
Poria cocos medicinal materials fat soluble component finger-print examination criteria draft
According to " appendix a VID of Chinese pharmacopoeia version in 2005 high effective liquid chromatography for measuring.
Chromatographic condition and system suitability test are filling agent with the octadecylsilane chemically bonded silica, WatersSymmetry 110A C
18(250mm * 4.6mm, 5 μ m) chromatographic column; With the acetonitrile is mobile phase A; With 0.1% phosphoric acid is Mobile phase B; Carry out wash-out according to the gradient in the table 1; Flow velocity is 1.0ml/min, and the detection wavelength is 210nm, and column temperature is 30 ℃, and theoretical cam curve is by 3 beta-hydroxies-wool steroid-7,9 (11), and 24-triolefin-21 acid (dehydrotrametenolic acid) peak calculates, and should be not less than 3000.
Poria cocos medicinal materials fat soluble component finger-print detects condition of gradient elution (table 1)
3 beta-hydroxies-wool steroid-7,9 (11) is got in the preparation of reference substance solution, and the sour reference substance of 24-triolefin-21 is an amount of, and accurate the title decides, and makes the solution that every 1ml contains 1mg with acetonitrile, shakes up, promptly.
The about 1.5g of Poria cocos medicinal powder (crossing sieve No. 4) is got in the preparation of need testing solution, and accurate the title decides, and puts in the 50ml tool plug triangular flask, the accurate 25ml methyl alcohol that adds, and close plug shakes up, and cold soaking 1.0hr after the jolting, filters with 0.2 μ m miillpore filter, promptly.
Accurate respectively reference substance solution, each the 20 μ l of need testing solution of drawing of determination method inject liquid chromatograph, measure, and write down 85 minutes chromatogram, promptly.
Test sample finger-print and reference fingerprint relatively identical chromatographic peak should occur in corresponding retention time, adopt finger-print similarity software evaluation, and its similarity should be not less than 0.8.
Finger-print is described: finger-print should detect at least 28 tangible chromatographic peaks, and wherein No. 24 peaks are 3 beta-hydroxies-wool steroid-7,9 (11), the sour peak of 24-triolefin-21, and Poria cocos medicinal materials fat soluble component finger-print common pattern is seen Fig. 1.
The sign of Luotian, Hubei Poria cocos characteristic fingerprint pattern: finger-print presents tangible 4 groups of peaks, the chromatographic peak of 5min～20min wherein, and promptly No. 1～7, chromatographic peak is lower; 20min～28.5min has 11 tangible chromatographic peaks, i.e. 8～No. 15 chromatographic peaks, and general 15#＞＞11# (13#)＞12#＞9# (17#) peak; 28.5min～48min has 5 tangible chromatographic peaks, i.e. 16～No. 23 chromatographic peaks, and 23#＞＞21#＞22#＞19# (20#) peak; 50min～62min has 5 tangible chromatographic peaks, i.e. 24～No. 28 chromatographic peaks, and wherein 24,26,27, No. 28 chromatographic peaks are the trend that reduces successively.In whole finger-print, chromatographic peak 15,23 and 24 is three higher peak, and is general highly for hanging down (see figure 1) successively.
The present invention's distinctive points and advantage compared with prior art
Innovative point (1) determines that 210nm is the detection wavelength of Poria cocos medicinal materials fat soluble component finger-print, and this method is easy, stable, precision is high and favorable reproducibility.
We select for use the 242nm of most of bibliographical informations as detecting wavelength, do the major part of liposoluble constituent finger-print research and investigated project, find in test, the different medicinal parts of Poria cocos medicinal material there are differences, very weak as a lot of triterpenic acid constituents in Poria cocos piece and the fuling peel in the uv absorption at 242nm place, having only just has stronger absorption endways, according to the three-dimensional collection of illustrative plates (see figure 2) of collecting, and collect 210nm simultaneously, 215nm, 220nm, 230nm, the collection of illustrative plates (see figure 3) at 242nm wavelength place, integrated survey, the Poria cocos medicinal materials fat soluble component is in the quantity of information maximum at 210nm wavelength place, and therefore last definite 210nm is the detection wavelength of Poria cocos medicinal materials fat soluble component finger-print.
We point out out the Poria cocos retention time at No. 24 peaks, total peak of 54.13 minutes according to the method for LC-MS innovative point (2): 3 beta-hydroxies-wool steroid-7,9 (11), 24-triolefin-21-acid is reference substance, Poria cocos to each place of production, the whole nation carries out " the chromatographic fingerprints of Chinese materia medica similarity evaluation system that finger-print detects and Poria cocos similarity in all parts of the country is recommended according to pharmacopoeia commission, the 2004A version " software, estimate similarity value between the sample of the different places of production except that sample is produced in Guizhou, the sample difference in its excess-three place of production is little, finally, the quality of medicinal material that produce in Hubei is stable, the active constituent content height, the best in quality; Two batches of medicinal materials are produced in the Hunan, it is close that medicinal material is produced in chromatographic peak distribution trend and area peak value and Hubei, it is little apart from closing on the quality of medicinal material difference that the medicinal material place of production is described, produce in the medicinal material collection of illustrative plates except that a characteristic chemical constituent that is different from other place of production medicinal material is arranged in Guizhou, the medicinal material basically identical in all the other compositions and Hubei, two places of production, Hunan, medicinal materials fingerprint chromatographic peak distribution and Hubei are produced in Yunnan, the Hunan is suitable, but part chromatographic peak content is obviously on the low side.
The finger-print peak quantity of information of innovative point (3) the present invention research is many than prior art, and utilize the LC-MS technology that 28 chromatographic peaks in the Poria cocos medicinal materials fat soluble component finger-print are identified, make the finger-print composition of foundation clearer and more definite, method is science, reliable more.(seeing Table 2)
The data message (table 2) of 28 chromatographic peaks in the HPLC spectrogram
Annotate: in the peak of table 2 numbered bin, 1) " a " expression infers resulting structures according to the retention time of document; 2) " b " expression is according to the structure of molecular weight and the supposition of triterpene compound cleavage of mass spectrum rule.
The specific implementation process of 17 batch samples is as follows
One, the collection of Poria cocos medicinal material sample
This product main product in Hubei, province such as Anhui, Henan, Yunnan, Guizhou, Sichuan, we have collected 17 batch samples altogether.
Two, 17 parts of Poria cocos medicinal materials fat soluble component finger-prints in all parts of the country are seen Fig. 4.
Three, Fig. 5 is seen in the contrast of tuckahoe fat-soluble finger-print in all parts of the country.
Four, the partly total peak of tuckahoe fat-soluble finger-print in all parts of the country similarity matched data: the partly total peak of 17 batches of Poria cocos (individual) liposoluble fingerprint similarity matched data sees Table 3; The partly total peak of 17 batches of Poria cocos (skin) liposoluble fingerprint similarity matched data sees Table 4.
Innovative point (4): No. 2 medicinal materials in Luotian, Hubei are originated from the right mountain of three groups of new protective embankments, the yellow family in triumph town, Luotian, Hubei shop, its Poria cocos and the fuling peel liposoluble constituent finger-print and the reference fingerprint similarity of appropriate section partly is all higher, choose these two collection of illustrative plates and carry out a fuling peel and a Poria cocos medicinal material part finger-print signature analysis, see Fig. 6 as representative collection of illustrative plates; For ease of recognizing and analyzing relatively, whole chromatogram is divided into I, II, four districts of III, IV, 28 chromatographic peaks of number in the figure are the total chromatographic peak of 17 parts of Poria cocos medicinal materials fat soluble component finger-print.
Figure below among Fig. 6 is the enlarged drawing of last figure.
Analysis result to Fig. 6 is described in detail as follows: total composition is higher than individual content in the skin; Do not have in having simultaneously characteristic chemical constituent in the skin and be.
1. the I district has 7 total peaks (please marking in the drawings), can find out by the contrast collection of illustrative plates that amplifies, during fuling peel and Poria cocos are individual this seven chromatographic peaks are arranged all, 1# peak peak heights in a skin and a medicinal material is suitable, can judge distribution is all arranged in whole sclerotium, and other 6 chromatographic peaks (peak value in skin of 2#～7#) therefore can judge that these six compositions are mainly derived from skin portion apparently higher than Poria cocos.
2. the II district has 11 total peaks, and wherein significantly chromatographic peak has peak 9,11,12,13,15, No. 17 peaks.Peak 11#, 15# and the 17# content in skin is higher than sclerotium far away as can be seen, illustrate that these three compositions mainly contain in skin, and the content of other three compositions in skin also is higher than sclerotium.
3. there are five total peaks in the III district, the peak height trend of each chromatographic peak in skin and whole poria cocos sclerotium chromatogram is basic identical, just the content in the skin is higher than sclerotium, but the 23# peak is compared with other peak in sclerotium, content is higher relatively, with more approaching in the skin, illustrate that this composition content in sclerotium is higher relatively.In addition, a tangible chromatographic peak (peak A, non-total peak) is arranged behind the 23# peak, and content is very low in sclerotium in the finger-print of skin, this composition can be used as the characteristic chemical constituent that fuling peel is different from sclerotium.
4. there are five significantly total peaks in the IV district, and is similar by the distribution trend of these five chromatographic peaks in two medicinal parts, show that these five compositions are present in the whole poria cocos sclerotium, but the content in the skin is apparently higher than sclerotium.In skin portion medicinal material collection of illustrative plates, before No. 24 peaks the tangible chromatographic peak (peak B, non-total peak) of one group of tool acromion is arranged, and in sclerotium, almost do not detect, this two characteristic chemical constituents that composition is a fuling peel are described.In addition, the chromatographic peak peak value that No. 28 peaks occur later in the sclerotium is all lower, so think that these compositions are mainly derived from the fuling peel part.
Claims (3)
1. the fingerprint atlas detection method of a tuckahoe fat-soluble component, adopt the HPLC-UV chromatography, simultaneously finger-print detection wavelength, chromatographic column, moving phase, column flow rate, column temperature, chromatographic condition time, blank moving phase, blank solvent, test sample preparation method are investigated, selecting the relative retention time at each total peak, relative percentage area, relative percentage height, spectrum similarity coefficient for use is characterization parameter, is the detection wavelength of Poria cocos medicinal materials fat soluble component finger-print with 210nm; It is characterized in that: with the octadecylsilane chemically bonded silica filling agent, Waters Symmetry 110A C
18250mm * 4.6mm, 5 μ m chromatographic columns; With the acetonitrile is mobile phase A; With 0.1% phosphoric acid is Mobile phase B; Carry out wash-out according to the gradient in the elution requirement; Flow velocity is 1.0ml/min, and column temperature is 30 ℃, and theoretical cam curve is by 3 beta-hydroxies-wool steroid-7,9 (11), and 24-triolefin-21 acid (dehydrotrametenolic acid) peak calculates, and is not less than 3000;
Elution requirement is as follows:
Time (min) mobile phase A (%) Mobile phase B (%)
0～70 45→95 55→5
70～85 95 5。
2. the fingerprint atlas detection method of tuckahoe fat-soluble component as claimed in claim 1, it is characterized in that: point out out the Poria cocos retention time at No. 24 peaks, total peak of 54.13 minutes according to the method for the LC-MS of liquid chromatograph-mass spectrometer coupling: 3 beta-hydroxies-wool steroid-7,9 (11), 24-triolefin-21-acid is reference substance, Poria cocos to each place of production carries out " the chromatographic fingerprints of Chinese materia medica similarity evaluation system that finger-print detects and various places Poria cocos similarity is recommended according to pharmacopoeia commission, the 2004A version " software, estimate the similarity value between the sample of the different places of production.
3. the fingerprint atlas detection method of tuckahoe fat-soluble component as claimed in claim 1 is characterized in that: determine 28 chromatographic peaks, utilize the LC-MS technology to carry out Poria cocos medicinal materials fat soluble component finger-print and detect.
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| CN104062306B (en) * | 2014-06-26 | 2016-06-01 | 劲牌有限公司 | The mass spectrum rapid analysis method of pesticide residue in a kind of fruit and vegetable |
| CN105486786B (en) * | 2016-01-28 | 2017-10-27 | 杏辉天力（杭州）药业有限公司 | A kind of detection method of fuling triterpene class compound |
| CN105974030B (en) * | 2016-07-22 | 2018-09-14 | 江苏康缘药业股份有限公司 | The method for building up of triterpenes components finger-print in a kind of GUIZHI FULING JIAONANG |
| CN110441442A (en) * | 2019-09-20 | 2019-11-12 | 广东一方制药有限公司 | A kind of Poria cocos standard decoction UPLC characteristic spectrum construction method and detection method |
| CN115078582A (en) * | 2022-06-21 | 2022-09-20 | 山东宏济堂制药集团股份有限公司 | Method for determining pachymic acid A and pachymic acid B contents in Poria by one-measurement-multiple-evaluation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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-
2007
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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Non-Patent Citations (17)
| Title |
|---|
| 丁岗 |
| 丁岗 王振中 章晨峰 盛龙生.茯苓中三萜酸类成分HPLC指纹图谱的初步研究.中国中药杂志27 10.2002,27(10),756-758. * |
| 于能江 |
| 于能江 郭继芬 赵毅民 张永祥.高效液相色谱-二极管阵列检测-质谱法鉴定茯苓中的三萜成分.分析化学32 7.2004,32(7),866-870. * |
| 张永祥.高效液相色谱-二极管阵列检测-质谱法鉴定茯苓中的三萜成分.分析化学32 7.2004,32(7),866-870. |
| 张继华.茯苓皮中三萜酸类成分HPLC指纹图谱的方法学研究.中药新药与临床药理16 1.2005,16(1),53-55. |
| 曹君 |
| 杨世林 |
| 段启 |
| 王少军 |
| 王少军 段启 曹君 杨世林 龚千锋 张继华.茯苓皮中三萜酸类成分HPLC指纹图谱的方法学研究.中药新药与临床药理16 1.2005,16(1),53-55. * |
| 王振中 |
| 盛龙生.茯苓中三萜酸类成分HPLC指纹图谱的初步研究.中国中药杂志27 10.2002,27(10),756-758. |
| 章晨峰 |
| 赵毅民 |
| 郭继芬 |
| 龚千锋 |
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