CN101084993B

CN101084993B - Medicinal composition containing dalbergia and storax, preparation method and preparation

Info

Publication number: CN101084993B
Application number: CN2006100141931A
Authority: CN
Inventors: 李旭; 郑永锋; 范立君
Original assignee: Tianjin Tasly Pharmaceutical Co Ltd
Current assignee: Tasly Pharmaceutical Group Co Ltd
Priority date: 2006-06-08
Filing date: 2006-06-08
Publication date: 2012-03-07
Anticipated expiration: 2026-06-08
Also published as: CN101084993A

Abstract

The invention discloses a Chinese medicinal composition for treating coronary disease, which is prepared from raw material herbs of root of red rooted saliva, notoginseng, rosewood oil and safflower or salvia miltiorrhizae, notoginseng, liquidambar orientalis mill and safflower, and the preparation process comprises the steps of mixing red-rooted salvia, notoginseng, safflower to obtain water extract or alcohol extract, subjecting the extract to preliminary settlement treatment, further subjecting the extract to ultrafiltration treatment, concentrating the ultrafiltrate, charging rosewood oil or liquidambar orientalis mill, and producing preparation through the conventional process.

Description

A kind of pharmaceutical composition, method for preparing and preparation that contains Lignum Dalbergiae Odoriferae and Styrax

Technical field

The present invention relates to a kind of Chinese medicine composition of using the ultrafiltration technology preparation.Particularly, the present invention relates to a kind of Chinese medicine composition of using the treatment coronary heart disease of ultrafiltration technology preparation.

Background technology

FUFANG DANSHEN PIAN is that Chinese Pharmacopoeia is gone through the kind recorded of version, has the effect of blood circulation promoting and blood stasis dispelling, regulating QI to relieve pain, be used for feeling oppressed in the heart, angina pectoris, and be medicine commonly used clinically at present.Its traditional handicraft is: gets Radix Salviae Miltiorrhizae and extracts 3 times, added alcohol reflux 1.5 hours the 1st time, filter, and filtrate recycling ethanol, being concentrated into relative density is 1.30 (55～60 ℃); Add 50% alcohol reflux 1.5 hours the 2nd time, filter.Add water for the 3rd time and refluxed 2 hours, filter, merge filtrating the 2nd time, the 3rd time, reclaim ethanol, being concentrated into relative density is 1.40 (55～60 ℃), and with the 1st time concentrated solution merging, mixing, processing relative density is the extractum of 1.35～1.39 (55 ℃).Radix Notoginseng powder is broken into fine powder, mixes all with Radix Salviae Miltiorrhizae extractum, drying is processed granule.With Lignum Dalbergiae Odoriferae oil or Styrax porphyrize, be mixed with above-mentioned granule, be pressed into 1000, or sugar coating, promptly get.

Though above-mentioned technology can make most effective ingredient be able to keep, and also can remove the impurity of a great deal of, this technology is adopted in discovering in recent years, and making much has very big loss with relevant polysaccharose substance and the many trace element of immunity; And the production cycle of this production technology is long, complex process, and production cost is high, and finished product stability is poor, is prone to produce environmental pollution.

Membrane separation technique (Membrane Separation Technique) is an emerging high efficient separation technology, by internationally recognized be a most rising great high production tech of mid-term 20 end of the centurys to 21 century.Ultrafiltration (Ultrafiltration, UF) technology is a kind of membrane separation technique, its ultimate principle is to utilize fenestra selectivity sieve performance, with separation, purification and condensed matter.Hyperfiltration process is that the anisotropic membrane (being asymmetric membrane) that utilizes macromolecular material to process is a filter medium, under normal temperature condition, relies on certain pressure and flow velocity, makes flow of solution through face, forces low molecular weight substance to see through film, and polymer substance is trapped.

Because hyperfiltration process is a physical method; Having not must repeated heating; Do not have " phase " to change, the probability of destroying effective ingredient is few than other universal method, characteristics such as technological process weak point; Thereby its be applied to extract in the research of pharmaceutically active ingredient become increasingly active, portioned product moves towards commercial production from laboratory research.Human ultrafiltrations such as 304 Wang Shiling of hospital of PLA are extracted effective ingredient baicalin in the Radix Scutellariae, and the result shows that ultrafiltration is excellent than well-established law all aspect productive rate, purity, and a ultrafiltration can reach the injection requirement; Do not need again row refining; Technology is simple, and the production cycle can shorten 1～2 times of (Wang Shiling, Zheng Dianbao " ultrafiltration is extracted the preliminary investigation of baicalin "; Chinese patent medicine research, 1988 (3): 5).Wang Shiling etc. have also further studied the optimum process condition of ultrafiltration extraction baicalin, and it is the key that improves baicalin yield and quality that the experimental result proof is selected the ultrafilter membrane in suitable aperture (molecular cut off is 6000～10000) for use, the fluid temperature that raises simultaneously or reduction concentration; Strict control pH value (pH=1.5 during acidify; PH=7.0 when alkali dissolves), can significantly improve ultrasiltrated rate, obtain best output effect (Wang Shiling; " ultrafiltration is once extracted the technical study of baicalin "; Chinese patent medicine, 1994,16 (3): 2).Xu Jinlin etc. are used for the preparation of phytic acid with ultrafiltration (PS membrane, molecular cut off 6000), and the phytic acid yield is 86.4%; Improve 12.6% than conventional phytate method, and ultrafiltration gained phytic acid contains Phos hardly, appearance transparent is close to colourless (Xu Jinlin; Xu Jie, Wang Yuanjin " membrane separation technique prepares the research of phytic acid ", Chinese Journal of Pharmaceuticals; 1994,25 (4): 150).He Changsheng etc. use hyperfiltration technique separation and purification steviol glycosides, and adopting ultrathin plate-type hyperfiltration device and molecular cut off is that 10000 CAM (CA film) purifies field experimentation to steviol glycosides, and its technological process is rationally feasible.The ultrafilter stable performance, the decoloration performance and the removal of impurity of film are respond well, bubble problem (He Changsheng in the time of can solving deposition that steviol glycosides usually occurs in producing and embedding preferably; Wang Ping Nan, Zhu Shanshan " applied research of steviol glycosides ultrafiltration ", water technology; 1994,20 (2): 89).Huang is improved oneself and is adopted the refining sasanguasaponin of ultrafilter membrane (molecular cut off is 4000 and 10000 PS membrane), compares with the domestic bleaching that mostly adopts, recrystallize method, the alcohol ether sedimentation method and the basic salt sedimentation method, and the ultrafiltration flow process is simple; Efficient is high, and expense is low, to removing oils and fats, pigment, saccharide and the strong impurity of other hydrophilic in the thick sasanguasaponin; Can both producing a desired effect, (Huang is improved oneself; " ultrafiltrationmembrane process is made with extra care the sasanguasaponin pre-test " water technology, 1995,21 (2): 99).Guo Li Wei of Nanjing University of Traditional Chinese Medicine etc. has relatively studied water alcohol method and ultrafiltration is clarified the influence of Fructus Corni preparation to its preparation ingredient; The result confirms that ultrafiltration is more effective to saccharide impurity in the removal medicinal liquid; Molecular cut off is that 10000 ultrafilter membrane does not have obviously influence to meliatin (molecular weight is 384), makes meliatin loss about 50% (Guo Liwei, Peng Guoping but molecular cut off is 1000 film; Pan Yang etc. " the refining comparative study that contains the Fructus Corni Chinese medicine preparation of water alcohol method and membrane separation process "; Chinese patent medicine, 1999,21 (2): 59).Wang Chengzhang etc. adopt ultrafiltrations (PS membrane, molecular cut off 30000) and polyamide absorb-elute method to the ethanol extract of Folium Ginkgo separate, purification, detect through HPLC (HPLC); The ginkgetin salidroside content is about 45%, and yield is 0.5%～0.7%, and more conventional steam distillation, organic solvent extraction method are excellent; And in ultrafiltration technology, can reduce discharge of wastewater, the protection environment reduces production costs; (Wang Chengzhang, Yu Qing, Tan Weihong etc. " application of ultrafiltration in purification Folium Ginkgo flavone glycoside " increase economic efficiency; The forestry science and technology communication, 1997, (2): 21).

Though hyperfiltration technique is applied to the production of Chinese medicine preparation its unique advantage is arranged, its degree of applying is still very limited, traces it to its cause, and remains in following problem:

(1) medicinal herb components is complicated, and particularly many compound preparations, effective ingredient are also unclear fully, therefore before hyperfiltration technique being applied to Chinese herbal and crude drugs preparations, need carry out very deep research.For example because the complexity of composition, do not carry out a large amount of pharmacology and clinical research test fully the evaluation ultrafiltration to Chinese medicine preparation in before the drug effect influence degree of each composition, can not ultrafiltration be applied to the production of most of Chinese medicine preparation.

(2) kind of membrane material is few, and membrane aperture distributes wide, and performance is understable.Used ultrafilter membrane material has cellulose acetate, polyacrylonitrile, polysulfones, SPSF, polysulfonamides etc. in Chinese medicine preparation production.By its affinity classification, be broadly divided into two types: hydrophobic film material and hydrophilic film material to water.Hydrophilic film such as cellulose acetate, SPSF material is few to solute absorption, and molecular cut off is less, but poor heat stability, mechanical strength, chemical proof property, antibacterium erosiveness are not high usually; Hydrophobic film materials such as polysulfones, mechanical strength is high, high temperature resistant, anti-solvent, anti-biodegradation, but because of containing a large amount of hydrophobicity genes or chain link in the strand, and have more electrostatic charge, thereby the permeable speed of film is low, contamination resistance is lower.

(3) pollution problem of film is to hinder hyperfiltration technique is moved towards the commercial Application stage by laboratory research biggest obstacle.In the ultra-filtration process of Chinese medicine preparation, when not good as if the medicinal liquid pretreating effect, face is prone to pollute, and fenestra stops up, and making permeation flux is that productivity ratio descends, even cisco unity malfunction, the production efficiency reduction, and cost rises, and causes shorten the service life of film.

(4) system of selection of membrane module is not set up as yet, the optimization of still needing of ultrafiltration parameter.The factor that influences the ultrafiltration effect is a lot, comprises the selection of membrane module, the cleaning method after the definite and ultrafilter of technological parameter uses etc.Therefore be applicable to ultrafiltration apparatus and the operating procedure that the ultrafiltration of Chinese medicine system is used, remain further to be studied.

The relevant practical application that utilizes ultrafiltration to prepare FUFANG DANSHEN PIAN, present document does not have any report as yet.Particularly, utilize ultrafiltration to carry out suitability for industrialized production is a technical barrier in this area always.

The inventor is through the effort of long-term and unremitting ground; Through a large amount of experimental datas are analyzed; Verified that ultrafiltration prepares the feasibility of medicine of the present invention, and confirmed suitable process condition, for the suitability for industrialized production of utilizing ultrafiltration to carry out medicine of the present invention provides concrete solution.

Summary of the invention

The Chinese medicine that the purpose of this invention is to provide the treatment coronary heart disease of ultrafiltration preparation technology's preparation that a kind of impurity is few, loss of effective components is little, cost is low; It has overcome the deficiency of prior art; Solve the difficult problem of suitability for industrialized production ultrafiltration technology condition operability, and on prescription, increased the cardiac stimulant effect of QI invigorating.

Another object of the present invention is to provide a kind of preparation by the above-mentioned composition preparation.

Another purpose of the present invention is to provide a kind of dropping pill formulation by the above-mentioned composition preparation.

The present invention realizes through following technical step: with Radix Salviae Miltiorrhizae, Radix Notoginseng, Lignum Dalbergiae Odoriferae oil and Flos Carthami or Radix Salviae Miltiorrhizae, Radix Notoginseng, Styrax and Flos Carthami is crude drug, prepares according to following steps:

(1) Radix Salviae Miltiorrhizae, Radix Notoginseng, Flos Carthami are mixed or process water extract or alcohol extract separately;

(2) described extracting solution being carried out predefecation handles;

(3) further described extracting solution is carried out hyperfiltration treatment;

(4) ultrafiltrate is concentrated, add Lignum Dalbergiae Odoriferae oil or Styrax, process preparation by conventional method.

The proportioning of above-mentioned raw materials medicine is counted by weight: 20～97 parts of Radix Salviae Miltiorrhizaes, 2～79 parts of Radix Notoginseng, Lignum Dalbergiae Odoriferae oil or 0.2～3 part of Styrax, 3～15 parts on Flos Carthami; Be preferably: 63.0～94.0 parts of Radix Salviae Miltiorrhizaes, 4.0～35.0 parts of Radix Notoginseng, Lignum Dalbergiae Odoriferae oil or 0.5～2.0 part of Styrax, 4～10 parts on Flos Carthami; More preferably: 75.2～90 parts of Radix Salviae Miltiorrhizaes, 9～23.5 parts of Radix Notoginseng, Lignum Dalbergiae Odoriferae oil or 0.5～1.3 part of Styrax, 5～8 parts on Flos Carthami.

In the technical step of the present invention (1), alcohol extract can be the extracting solution of lower alcohol such as methanol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol, isobutanol of variable concentrations etc. or the extracting solution of its mixture.Carrying out next step predefecation after alcohol extract can not concentrate or suitably concentrate handles.

In the technical step of the present invention (2); Preliminary clarifying treatment can be carried out coarse filtration with general material such as gauze, tiffany etc.; The also available material such as the ceramic membrane of specialty carry out microfiltration; Also can behind high speed centrifugation, obtain supernatant, adsorption clarification such as also available flocculating agent such as flocculate with chitosan clarifier, 101 fruit juice clarifiers, ZTC1+1 natural clarifying agent, Ovum Gallus domesticus album flocculating agent and remove particle bigger in the medicinal liquid, also available alcohol deposition method is removed most impurity.Both can singly use above-mentioned defecation method, but also Combined application, coarse filtration-adsorption clarification for example, adsorption clarification-high speed centrifugation, coarse filtration-microfiltration, coarse filtration-precipitate with ethanol etc.After can not concentrating or suitably concentrate, the solution that predefecation is handled carries out next step ultrafiltration; Preferably do not concentrate the ultrafiltration of promptly carrying out next step.

In the technical step of the present invention (3); The used ultrafilter membrane of ultrafiltration can be cellulose diacetate film (CA), three cellulose acetate membrane (CTA), cyanoethyl cellulose film (CN-CA), PS membrane (PS), sulfonated polysulfone membrane (SPS), poly (ether sulfone) film (PES), sulfonated polyether sulfone film (SPES), polysulfonamides film (PSA), phenolphthalein side group polyarylsulfone (PAS) film (PDS), polyvinylidene fluoride film (PVDF), polyacrylonitrile film (PAN), polyimide film (N), cellulose membrane, methyl methacrylate-acrylonitrile copolymer film (MMA-AN), polyacrylonitrile/cellulose diacetate (PAN/CA) blend film; The ultrafilter membrane that dynamically forms, and the Modified Membrane of above-mentioned film.Be preferably cellulose diacetate film (CA), three cellulose acetate membrane (CTA), PS membrane (PS), sulfonated polysulfone membrane (SPS), poly (ether sulfone) film (PES), sulfonated polyether sulfone film (SPES), polysulfonamides film (PSA), polyvinylidene fluoride film (PVDF), polyacrylonitrile film (PAN).

The molecular cut off of above-mentioned ultrafilter membrane is generally 6000～80000, is preferably 10000～70000, and the best is 20000～50000.

Ultrafiltration both can be adopted cross flow filter, also can adopt dead-end filtration, but preferred cross flow filter.

The operating condition of ultrafiltration technology is following:

(1) the inlet pressure of ultrafiltration is 0.1～0.5MPa, is preferably 0.1～0.35Mpa, and the best is 0.25～0.35Mpa; The liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.5～0.25kPa.The ultrafiltration initial stage is adopted lower pressure, slowly boosts then; In ultra-filtration process, adopt the periodic pressure fluctuation, the pressure wave moment is 0.1～0.2Mpa.

(2) the feed liquid flow velocity is 1.0～4.0m/s, is preferably 2.0～3.0m/s.In the ultra-filtration process, adopt the fluctuation of periodicity flow so that in membrane channels, produce pulsating flow or non-stationary flow, the flow speed wave moment is 1.0～2.0m/s.

(3) feed high-pressure inert gas such as nitrogen in the ultrafiltration system discontinuous, form the gas-liquid stream of pulses, the cycle is that 0.5h～2h ventilates once, each 1 minute.

(3) feed temperature is 15～50 ℃, is preferably 20～40 ℃.

(4) when feed liquid stock solution is concentrated 1/15～1/5, add water or dilute alcohol solution ultrafiltration 1～2 time again; Be preferably when feed liquid stock solution is concentrated 1/12～1/8, add water or dilute alcohol solution ultrafiltration 1～2 time again.

(5) pH value of feed liquid is controlled at 5～9, is preferably 6.0～7.5;

(6) backwash condition: backwashing pressure is 0.15～2.5MPa, and backwashing period is that 0.5～1.5h, backwashing time are 1min～10min.When the method for alternately recoil is used in the ultrafiltration module parallel connection; Wherein a cover or a few cover carry out normal ultrafiltration and distribute the recoil ultrafilter membrane of another set of or several grip assemblies of a part of filtrating; Exchange is carried out behind the certain interval of time, generally is work 10～20min, recoil 30sec～3min.

(7) the Chemical cleaning cycle is 0.5 month～February; The Chemical cleaning medicament is generally diluted acid, diluted alkaline, surfactant; Be preferably diluted alkaline, 0.5%～4.0% sodium hydroxide for example, the mixed solution of 1.5% sodium hydroxide and 2% sodium hypochlorite etc.; PH value is 10～12, and cleaning pressure is 0.05～1.0MPa.After cleaning with chemical, the flushing of reuse water is near neutral.

In ultra-filtration process; Both periodic pressure oscillation or the fluctuation of periodicity flow or periodicity fed noble gas separately; Also can unite use, i.e. periodic pressure fluctuation and periodically flow fluctuation associating use, perhaps the periodic pressure fluctuation is with periodically feeding noble gas unites use; Perhaps periodically the flow fluctuation is with periodically feeding noble gas unites use, and perhaps the three unites use together.

In the technical step of the present invention (4), ultrafiltrate is condensed into extractum after, process preparation by conventional method again.For example; Can be mixed and made into tablet, capsule, granule, oral liquid, slow releasing preparation, controlled release preparation, gel, ointment, ointment, cream, suppository, injection, injectable powder, patch, drop pill, suspensoid with adjuvant on any or more than one pharmaceuticss such as starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, Polyethylene Glycol, magnesium stearate, micropowder silica gel, xylitol, lactose, glucose, glycine, mannitol, glycine etc., be preferably drop pill.

Drop pill of the present invention is prepared from as active component and adjuvant above-mentioned Chinese medicine composition, perhaps is prepared from as active component and substrate adjuvant and plasticity adjuvant above-mentioned Chinese medicine composition.

The substrate adjuvant of drop pill can be used Polyethylene Glycol, can be one or more the mixture in cetomacrogol 1000, polyethylene glycol 1500, Macrogol 4000, the polyethylene glycol 6000; Preferred Macrogol 4000, polyethylene glycol 6000; The best is a polyethylene glycol 6000; Addition is 2～10 times of extractum weight.

The preferred for preparation method is: after step (d) ultrafiltrate is condensed into extractum; Directly with adjuvant or with Borneolum Syntheticum or Lignum Dalbergiae Odoriferae oil or Styrax or Moschus or muscone and adjuvant mixed even after; Add the transconversion into heat material, move into the jar that drips of drop pill machine, in medicine liquid droplet to condensed fluid such as liquid paraffin or the methyl-silicone oil; Remove condensed fluid, select ball.

Wherein: adjuvant is a Polyethylene Glycol-6000, and 53～58 ℃ of its condensation points, addition are extractum or extractum and Borneolum Syntheticum or Lignum Dalbergiae Odoriferae oil or Styrax or Moschus or muscone weight 2～6 times; Changing the material temperature is 60～100 ℃; The temperature of condensed fluid is 0～10 ℃, and the best is 5～10 ℃; Ball heavily is 5～50mg/ grain, diameter 1.95～4.29mm.

The substrate adjuvant of drop pill preferably from natural, the substrate adjuvant of plant origin particularly, also can contain the plasticity composition in this substrate adjuvant.Specifically, the substrate adjuvant is selected from following one or more adjuvant: at least a substrate adjuvant in pharmaceutically useful monosaccharide, oligosaccharide, polysaccharide, sugar ester, sugar alcohol, fruit acid, advanced higher fatty acid derivative, high fatty alcohol, polyhydric alcohol, carbamide, the polyethylene oxide derivant.

In the above-mentioned substance, monosaccharide such as D-ribose, fructose, glucose, xylose; Oligosaccharide such as trehalose, Raffinose, maltose; Polysaccharide such as agarose; Sugar ester such as sucrose ester, D-ribonic acid-gamma lactone; Sugar alcohol such as erythritol, sorbitol, xylitol, arabitol, isomalt, lactose; Fruit acid such as malic acid, citric acid; Advanced higher fatty acid derivative such as sodium stearate, tristerin, tripalmitin, Lac; High fatty alcohol such as hexadecanol, octadecanol; Polyhydric alcohol such as phenylglycol; Polyethylene oxide derivant such as polyoxyethylene monostearate, polyoxyethylene alkyl ether, and above-claimed cpd contain water of crystallization chemical compound etc.In the above material; The natural adjuvant of plant origin such as erythritol, sorbitol, fructose, D-ribonic acid-gamma lactone, arabitol, trehalose, D-ribose, low melting-point agarose, Lac, xylitol, Raffinose, glucose, malic acid, citric acid, isomalt, lactose, maltose, xylose, sucrose ester etc., and they contain the water of crystallization chemical compound; Chemosynthesis adjuvant and animal origin adjuvant such as phenylglycol, Polyethylene Glycol, hexadecanol, octadecanol, sodium stearate, tristerin, tripalmitin, carbamide, polyoxyethylene monostearate, polyoxyethylene alkyl ether.In the above-mentioned substance, especially preferably from following one or more adjuvant: sorbitol, xylitol, lactose, maltose, sucrose ester, and they contain the water of crystallization chemical compound.

Above-mentioned substrate adjuvant is mainly the natural adjuvant of plant origin, and the content that is meant the adjuvant of plant origin in adjuvant is more than 50 weight %, and the content of chemosynthesis adjuvant and animal origin adjuvant is no more than the natural adjuvant of plant origin.Preferably; The substrate adjuvant of drop pill of the present invention only uses the natural adjuvant of plant origin, perhaps is mainly the natural adjuvant of plant origin and only contains a spot of chemosynthesis adjuvant and animal origin adjuvant, and its content is below 50 weight %; Below the preferred 40 weight %, more preferably below the 30 weight %.The natural adjuvant of above-described so-called plant origin is meant as adjuvant itself and in plant cell or tissue, extracts, or by the material of plant extract through modifications such as derivatizations after the product of gained.So-called chemosynthesis adjuvant is meant synthetic micromolecule or the macromolecular compound that is obtained through chemical synthesis process by simple micromolecule.The natural adjuvant of so-called animal origin is meant as adjuvant itself and in the animal cell or tissue, extracts, or the material that extracts by animal through modifications such as derivatizations after the product of gained.

The substrate adjuvant of above-mentioned plant origin; Have now or in the future possibly have the synthetic article; If the synthetic article are identical or close with the character of the natural substrates adjuvant of original plant origin; Characteristic with safety non-toxic, then the natural substrates adjuvant of alternative plant origin is used as the natural substrates adjuvant that kind of above-mentioned plant origin.

For improving the formability of drop pill of the present invention, preferably also contain the plasticity composition in the adjuvant of drop pill of the present invention.As this plasticity composition, for example can enumerate and be selected from following one or more composition: the natural adjuvant of plant origin such as starch and derivant, cellulose and derivant thereof, arabic gum, dextran, chitin, sesbania gum, carrageenan, Ficus elastica, Furcellaran, tragakanta, carrageenin, tamarind gum, pectin, xanthan gum, alginic acid and salt thereof, dextrin, cyclodextrin, agar, lactose; Chemosynthesis adjuvant and animal origin adjuvant such as polyvinylpyrrolidone, crospolyvinylpyrrolidone, carbomer, polyvinyl alcohol, acrylic resin, poloxamer, silicon dioxide, gelatin etc.

Above-mentioned starch and derivant thereof such as pregelatinized Starch, modified starch, hydroxypropyl starch, carboxymethyl starch etc.Said cellulose and derivant thereof such as methylcellulose, microcrystalline Cellulose, sodium carboxymethyl cellulose, hydroxypropyl emthylcellulose, cross-linking sodium carboxymethyl cellulose, hydroxyethylmethyl-cellulose, hydroxyethyl-cellulose, hydroxypropyl cellulose.

In the plasticity composition, preferably from following one or more adjuvant: pregelatinized Starch, carboxymethyl starch, methylcellulose, sodium carboxymethyl cellulose, hydroxypropyl emthylcellulose, arabic gum, alginic acid, dextrin, cyclodextrin, agar, lactose.In addition, glyceryl monostearate, polyoxyethylene monostearate also can be used as the plasticity composition and other substrate supplementary product compatibility is used.

The composition of above-described so-called plant origin is meant as adjuvant itself and in plant cell or tissue, extracts, or by the material of plant extract through modifications such as derivatizations after the product of gained.So-called chemosynthesis adjuvant is meant synthetic micromolecule or the macromolecular compound that is obtained through chemical synthesis process by simple micromolecule.The adjuvant of so-called animal origin is meant as adjuvant itself and in the animal cell or tissue, extracts, or the material that extracts by animal through modifications such as derivatizations after the product of gained.

The plasticity composition of above-mentioned plant origin; Have now or in the future possibly have the synthetic article; If the synthetic article are identical or close with the character of the natural plasticity composition of plant origin; Characteristic with safety non-toxic, then the natural plasticity composition of alternative above-mentioned plant origin is used as the natural plasticity substrate adjuvant that kind of above-mentioned plant origin.

The substrate adjuvant in the above-mentioned substrate adjuvant that is selected from monosaccharide, oligosaccharide, polysaccharide, sugar ester, sugar alcohol, fruit acid, advanced higher fatty acid derivative, high fatty alcohol, polyhydric alcohol, carbamide, polyethylene oxide derivant etc., preferred plant source is selected to arrange in pairs or groups according to medicinal property with above-mentioned plasticity composition; It is preferably arranged in pairs or groups and is xylitol and starch, lactose and starch, xylitol and arabic gum, sucrose ester and glyceryl monostearate, sucrose ester and polyoxyethylene monostearate, sucrose ester and polyoxyethylene monostearate and cross-linking sodium carboxymethyl cellulose, sucrose ester and polyoxyethylene monostearate and cross-linking sodium carboxymethyl cellulose and silicon dioxide etc., but is not limited thereto.

The weight ratio of above-mentioned substrate adjuvant and plasticity composition is 1: 0～1: 1.5, is preferably 1: 0.1～1: 0.9, and the best is 1: 0.1～1: 0.5.

In the above-mentioned substrate adjuvant, xylitol is preferably 1: 0.2 with the ratio of the weight of starch～and 1: 0.3;

In the above-mentioned substrate adjuvant, lactose is preferably 1: 0.2 with the ratio of the weight of starch～and 1: 0.3;

In the above-mentioned substrate adjuvant, the ratio of the weight of xylitol and arabic gum is preferably 1: 0.2～and 1: 0.4;

In the above-mentioned substrate adjuvant, the weight ratio of sucrose ester and glyceryl monostearate is 1: 0.1～1: 1, and the best is 1: 0.5.

In the above-mentioned substrate adjuvant, the weight ratio of sucrose ester and polyoxyethylene monostearate is 1: 0.1 to 1: 1, and the best is 1: 0.5.

In the above-mentioned substrate adjuvant, the weight ratio of sucrose ester and polyoxyethylene monostearate and cross-linking sodium carboxymethyl cellulose is 1: (0.1～1): (0.1～1), the best are 1: 0.4: 0.6.

In the above-mentioned substrate adjuvant, the weight ratio of sucrose ester and polyoxyethylene monostearate and cross-linking sodium carboxymethyl cellulose and silicon dioxide is 15: (7～15): (0.1～2): (0.1～2), the best are 15: 11: 1: 1.

Above-mentioned drop pill substrate adjuvant is 1: 0.1～1: 1 with the ratio of the weight of extractum, is preferably 1: 0.1～1: 0.6, and the best is 1: 0.2～1: 0.4.

The preferred for preparation process conditions are: extractum mixes mixing time with the substrate adjuvant be 10～30 minutes; A mixed heating and melting temperature of extractum and substrate adjuvant or a system temperature are 45～95 ℃, are preferably 60～95 ℃; Liquid coolant is liquid paraffin, methyl-silicone oil or vegetable oil (Oleum Glycines, Semen Ricini wet goods) etc., is preferably liquid paraffin, methyl-silicone oil; The temperature of liquid coolant is-20～30 ℃, is preferably 0～18 ℃; Dropper mouth internal diameter is 1.0～4.0mm, is preferably 1.2～2.5mm; The difference of dropper mouth external diameter and internal diameter is less for well.

Through medicine of the present invention its beneficial effect is explained in the experiment of the protective effect of Ischemia and Reperfusion in vivo in Rats myocardial damage below.

1. animal model: Wistar strain male rat, open chest anesthetized is kept breathing, between left auricle and pulmonary conus, around left coronary artery, and carries out ligation.

2. method: rat is divided into 4 groups at random: 1. sham operated rats (sham-operated control), normal saline is irritated stomach, 1ml/ days, totally 4 days; 2. myocardial ischemia-reperfusion group (M-IR), it is the same to irritate the stomach method; 3. FUFANG DANSHEN PIAN group (with reference to the preparation of the method for 2000 editions FUFANG DANSHEN PIANs of Chinese Pharmacopoeia) was dissolved in the 1ml normal saline with 2g crude drug/kg/ days, and it is the same to irritate the stomach method; 4. drug group of the present invention was dissolved in the 1ml normal saline with 2g crude drug/kg/ days, and it is the same to irritate the stomach method.Not ligation of sham operated rats; Other group is in ligation perfusion again after 1 hour.Take out after injecting 1% her Wen orchid in the ligation left coronary artery once more before sacrifice of animal, ventricle, with the PBS rinsing, freezing 1 hour.After removing unnecessary tissue, 30 minutes (37 ℃) of dyeing in 1%TTC.With weight method calculating myocardium ischemia hazardous area (she does not dye the district by the Wen orchid), infarcted region (TTC does not dye the district).

3. the result of variations of myocardial infarct size

The myocardial infarction phenomenon was not seen in sham-operation in 7 hours; It is obvious that myocardial ischemia poured into after 6 hours myocardial infarction in 1 hour again; Medicine of the present invention can obviously dwindle the myocardial infarct size of myocardial reperfusion rat, shows that medicine of the present invention pours into the variation of myocardium cell necrosis property again to ischemic myocardial cells good protective action (seeing table 1) is arranged.

Table 1 is respectively organized the variation of myocardial infarct size

Annotate: compare with the M-IR group, ^*P＜0.05, ^*P＜0.01; Compare with the FUFANG DANSHEN PIAN group, ^△P＜0.05, ^{△ △}P＜0.01.

The specific embodiment

Below in conjunction with embodiment the present invention is done further elaboration.These embodiment only are used to the purpose of giving an example, rather than limit the present invention by any way.

Embodiment one

Crude drug adopts Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Lignum Dalbergiae Odoriferae oil 8g, Flos Carthami 50g.

Obtain the ethanol extract of Radix Salviae Miltiorrhizae and Flos Carthami with ethanol extraction Radix Salviae Miltiorrhizae and Flos Carthami, with this extracting liquid filtering, collect filtrating with gauze.It is that 6000 cellulose diacetate film carries out ultrafiltration that filtrating is used molecular cut off, and filter type adopts cross flow filter.The operating condition of ultrafiltration technology is: the inlet pressure of ultrafiltration is 0.1Mpa, and the liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.5kPa.The ultrafiltration initial stage is adopted lower pressure, slowly boosts then; In ultra-filtration process, adopt the periodic pressure fluctuation, the pressure wave moment is 0.1Mpa.The feed liquid flow velocity is 1.0m/s, in the ultra-filtration process, adopts periodically flow fluctuation so that in membrane channels, produce pulsating flow or non-stationary flow; The flow speed wave moment is 1.0m/s, feeds nitrogen in the ultrafiltration system discontinuous, forms the gas-liquid stream of pulses; Cycle is that 0.5h ventilates once each 1 minute.Feed temperature is 15 ℃, when feed liquid stock solution is concentrated 1/15, adds water or dilute alcohol solution ultrafiltration 1 time again, and the pH value of feed liquid is controlled at 5.Backwashing pressure is 0.15MPa, and backwashing period is that 0.5h, backwashing time are 1min.When the method for alternately recoil was used in ultrafiltration module parallel connection, wherein a cover or a few cover carried out normal ultrafiltration and distribute the recoil ultrafilter membrane of another set of or several grip assemblies of part filtrating, and exchange is carried out behind the certain interval of time, work 10min, recoil 30sec.The Chemical cleaning cycle is 0.5 month, and the Chemical cleaning medicament is the mixed solution of 0.5%～4.0% sodium hydroxide, 1.5% sodium hydroxide and 2% sodium hypochlorite, and pH value is 10～12, and cleaning pressure is 0.05MPa.After cleaning with chemical, the flushing of reuse water is near neutral.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.35～1.39 (55 ℃).Radix Notoginseng powder is broken into fine powder, mixes all with above-mentioned extractum, drying is processed granule, sprays into Lignum Dalbergiae Odoriferae oil, is mixed with above-mentioned granule, is pressed into 1000, or sugar coating, promptly gets.

Embodiment two

Crude drug adopts Radix Salviae Miltiorrhizae 558g, Radix Notoginseng 34g, Lignum Dalbergiae Odoriferae oil 8g, Flos Carthami 72g.

Obtain the ethanol extract of Radix Salviae Miltiorrhizae and Flos Carthami with ethanol extraction Radix Salviae Miltiorrhizae and Flos Carthami, carry out microfiltration, collect filtrating with ceramic membrane.It is that 80000 PS membrane carries out ultrafiltration that filtrating is used molecular cut off, and filter type adopts dead-end filtration.The operating condition of ultrafiltration technology is: the inlet pressure of ultrafiltration is 0.5Mpa, and the liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.25kPa.The ultrafiltration initial stage is adopted lower pressure, slowly boosts then; In ultra-filtration process, adopt the periodic pressure fluctuation, the pressure wave moment is 0.2Mpa.The feed liquid flow velocity is 4.0m/s, in the ultra-filtration process, adopts periodically flow fluctuation so that in membrane channels, produce pulsating flow or non-stationary flow; The flow speed wave moment is 2.0m/s, feeds nitrogen in the ultrafiltration system discontinuous, forms the gas-liquid stream of pulses; Cycle is that 2h ventilates once each 1 minute.Feed temperature is 50 ℃, when feed liquid stock solution is concentrated 1/5, adds water or dilute alcohol solution ultrafiltration 2 times again, and the pH value of feed liquid is controlled at 9.Backwashing pressure is 2.5MPa, and backwashing period is that 1.5h, backwashing time are 10min.When the method for alternately recoil was used in ultrafiltration module parallel connection, wherein a cover or a few cover carried out normal ultrafiltration and distribute the recoil ultrafilter membrane of another set of or several grip assemblies of part filtrating, and exchange is carried out behind the certain interval of time, work 20min, recoil 3min.The Chemical cleaning cycle is 2 months, and the Chemical cleaning medicament is the mixed solution of 0.5%～4.0% sodium hydroxide, 1.5% sodium hydroxide and 2% sodium hypochlorite, and pH value is 10～12, and cleaning pressure is 1.0MPa.After cleaning with chemical, the flushing of reuse water is near neutral.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.35～1.39 (55 ℃).Radix Notoginseng powder is broken into fine powder, mixes all with above-mentioned extractum, drying is processed granule, sprays into Lignum Dalbergiae Odoriferae oil; Be mixed with above-mentioned granule, add 3% polyvidone alcoholic solution system soft material, cross 18 mesh sieve system granules, 60 ℃ of dryings 30～45 minutes, granulate; Add Pulvis Talci, mixing fills in capsule, promptly gets.

Embodiment three

Crude drug adopts Radix Salviae Miltiorrhizae 360g, Radix Notoginseng 232g, Lignum Dalbergiae Odoriferae oil 8g, Flos Carthami 24g.

Obtain blended ethanol extract with ethanol extraction Radix Salviae Miltiorrhizae, Radix Notoginseng and Flos Carthami, with obtaining supernatant behind this extracting solution high speed centrifugation.It is that 50000 PS membrane carries out ultrafiltration that this liquid is used molecular cut off, and filter type adopts cross flow filter.The operating condition of ultrafiltration technology is: the inlet pressure of ultrafiltration is 0.35Mpa, and the liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.20kPa.The ultrafiltration initial stage is adopted lower pressure, slowly boosts then; In ultra-filtration process, adopt the periodic pressure fluctuation, the pressure wave moment is 0.2Mpa.The feed liquid flow velocity is 3.0m/s, in the ultra-filtration process, adopts periodically flow fluctuation so that in membrane channels, produce pulsating flow or non-stationary flow; The flow speed wave moment is 2.0m/s, feeds nitrogen in the ultrafiltration system discontinuous, forms the gas-liquid stream of pulses; Cycle is that 2h ventilates once each 1 minute.Feed temperature is 40 ℃, when feed liquid stock solution is concentrated 1/8, adds water or dilute alcohol solution ultrafiltration 2 times again, and the pH value of feed liquid is controlled at 7.5.Backwashing pressure is 2.5MPa, and backwashing period is that 1.5h, backwashing time are 10min.When the method for alternately recoil was used in ultrafiltration module parallel connection, wherein a cover or a few cover carried out normal ultrafiltration and distribute the recoil ultrafilter membrane of another set of or several grip assemblies of part filtrating, and exchange is carried out behind the certain interval of time, work 20min, recoil 3min.The Chemical cleaning cycle is 2 months, and the Chemical cleaning medicament is the mixed solution of 0.5%～4.0% sodium hydroxide, 1.5% sodium hydroxide and 2% sodium hypochlorite, and pH value is 10～12, and cleaning pressure is 1.0MPa.After cleaning with chemical, the flushing of reuse water is near neutral.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.35～1.39 (55 ℃).Drying is processed granule, sprays into Lignum Dalbergiae Odoriferae oil, is mixed with above-mentioned granule, adds an amount of magnesium stearate mix homogeneously, is pressed into 1000, or sugar coating, promptly gets.

Embodiment four

Crude drug adopts Radix Salviae Miltiorrhizae 500g, Radix Notoginseng 94g, Lignum Dalbergiae Odoriferae oil 6g, Flos Carthami 42g.

Radix Salviae Miltiorrhizae, the Radix Notoginseng and Flos Carthami medical material of coarse pulverization are added in the extraction pot, add 5 times of water gagings, decocted 2 hours, filter, filtering residue carries out the second time and extracts, and adds 4 times of water gagings, fries in shallow oil 1 hour, filters, and filtering residue discards, merging filtrate.It is that 6000 cellulose diacetate film carries out ultrafiltration that filtrating is used molecular cut off, and filter type adopts cross flow filter.The operating condition of ultrafiltration technology is: the inlet pressure of ultrafiltration is 0.1Mpa, and the liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.5kPa.The ultrafiltration initial stage is adopted lower pressure, slowly boosts then; In ultra-filtration process, adopt the periodic pressure fluctuation, the pressure wave moment is 0.1Mpa.The feed liquid flow velocity is 1.0m/s, in the ultra-filtration process, adopts periodically flow fluctuation so that in membrane channels, produce pulsating flow or non-stationary flow; The flow speed wave moment is 1.0m/s, feeds nitrogen in the ultrafiltration system discontinuous, forms the gas-liquid stream of pulses; Cycle is that 0.5h ventilates once each 1 minute.Feed temperature is 15 ℃, when feed liquid stock solution is concentrated 1/15, adds water or dilute alcohol solution ultrafiltration 1 time again, and the pH value of feed liquid is controlled at 5.Backwashing pressure is 0.15MPa, and backwashing period is that 0.5h, backwashing time are 1min.When the method for alternately recoil was used in ultrafiltration module parallel connection, wherein a cover or a few cover carried out normal ultrafiltration and distribute the recoil ultrafilter membrane of another set of or several grip assemblies of part filtrating, and exchange is carried out behind the certain interval of time, work 10min, recoil 30sec.The Chemical cleaning cycle is 0.5 month, and the Chemical cleaning medicament is the mixed solution of 0.5%～4.0% sodium hydroxide, 1.5% sodium hydroxide and 2% sodium hypochlorite, and pH value is 10～12, and cleaning pressure is 0.05MPa.After cleaning with chemical, the flushing of reuse water is near neutral.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.32～1.40 (55 ℃), spray into Lignum Dalbergiae Odoriferae oil, mix homogeneously, with the lactose fluidisation, drying is processed granule, promptly gets in the batch (-type) fluid bed.

Embodiment five

Crude drug adopts Radix Salviae Miltiorrhizae 290g, Radix Notoginseng 306g, Lignum Dalbergiae Odoriferae oil 6g, Flos Carthami 20g.

Radix Salviae Miltiorrhizae, the Radix Notoginseng and Flos Carthami medical material of coarse pulverization are added in the extraction pot, add 5 times of water gagings, decocted 2 hours, filter, filtering residue carries out the second time and extracts, and adds 4 times of water gagings, fries in shallow oil 1 hour, filters, and filtering residue discards, merging filtrate.It is that 80000 PS membrane carries out ultrafiltration that filtrating is used molecular cut off, and filter type adopts dead-end filtration.The operating condition of ultrafiltration technology is: the inlet pressure of ultrafiltration is 0.5Mpa, and the liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.25kPa.The ultrafiltration initial stage is adopted lower pressure, slowly boosts then; In ultra-filtration process, adopt the periodic pressure fluctuation, the pressure wave moment is 0.2Mpa.The feed liquid flow velocity is 4.0m/s, in the ultra-filtration process, adopts periodically flow fluctuation so that in membrane channels, produce pulsating flow or non-stationary flow; The flow speed wave moment is 2.0m/s, feeds nitrogen in the ultrafiltration system discontinuous, forms the gas-liquid stream of pulses; Cycle is that 2h ventilates once each 1 minute.Feed temperature is 50 ℃, when feed liquid stock solution is concentrated 1/5, adds water or dilute alcohol solution ultrafiltration 2 times again, and the pH value of feed liquid is controlled at 9.Backwashing pressure is 2.5MPa, and backwashing period is that 1.5h, backwashing time are 10min.When the method for alternately recoil was used in ultrafiltration module parallel connection, wherein a cover or a few cover carried out normal ultrafiltration and distribute the recoil ultrafilter membrane of another set of or several grip assemblies of part filtrating, and exchange is carried out behind the certain interval of time, work 20min, recoil 3min.The Chemical cleaning cycle is 2 months, and the Chemical cleaning medicament is the mixed solution of 0.5%～4.0% sodium hydroxide, 1.5% sodium hydroxide and 2% sodium hypochlorite, and pH value is 10～12, and cleaning pressure is 1.0MPa.After cleaning with chemical, the flushing of reuse water is near neutral.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.32～1.40 (55 ℃), with this extractum and an amount of magnesium stearate mix homogeneously, spray into Lignum Dalbergiae Odoriferae oil, be mixed with above-mentioned granule, compacting is wrapped film-coat in flakes.

Embodiment six

Crude drug adopts Radix Salviae Miltiorrhizae 210g, Radix Notoginseng 380g, Lignum Dalbergiae Odoriferae oil 4g, Flos Carthami 30g.

Radix Salviae Miltiorrhizae, the Radix Notoginseng and Flos Carthami medical material of coarse pulverization are added in the extraction pot, add 5 times of water gagings, decocted 2 hours, filter, filtering residue carries out the second time and extracts, and adds 4 times of water gagings, fries in shallow oil 1 hour, filters, and filtering residue discards, merging filtrate.It is that 50000 PS membrane carries out ultrafiltration that filtrating is used molecular cut off, and filter type adopts cross flow filter.The operating condition of ultrafiltration technology is: the inlet pressure of ultrafiltration is 0.35Mpa, and the liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.20kPa.The ultrafiltration initial stage is adopted lower pressure, slowly boosts then; In ultra-filtration process, adopt the periodic pressure fluctuation, the pressure wave moment is 0.2Mpa.The feed liquid flow velocity is 3.0m/s, in the ultra-filtration process, adopts periodically flow fluctuation so that in membrane channels, produce pulsating flow or non-stationary flow; The flow speed wave moment is 2.0m/s, feeds nitrogen in the ultrafiltration system discontinuous, forms the gas-liquid stream of pulses; Cycle is that 2h ventilates once each 1 minute.Feed temperature is 40 ℃, when feed liquid stock solution is concentrated 1/8, adds water or dilute alcohol solution ultrafiltration 2 times again, and the pH value of feed liquid is controlled at 7.5.Backwashing pressure is 2.5MPa, and backwashing period is that 1.5h, backwashing time are 10min.When the method for alternately recoil was used in ultrafiltration module parallel connection, wherein a cover or a few cover carried out normal ultrafiltration and distribute the recoil ultrafilter membrane of another set of or several grip assemblies of part filtrating, and exchange is carried out behind the certain interval of time, work 20min, recoil 3min.The Chemical cleaning cycle is 2 months, and the Chemical cleaning medicament is the mixed solution of 0.5%～4.0% sodium hydroxide, 1.5% sodium hydroxide and 2% sodium hypochlorite, and pH value is 10～12, and cleaning pressure is 1.0MPa.After cleaning with chemical, the flushing of reuse water is near neutral.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.32～1.40 (55 ℃),, spray into Lignum Dalbergiae Odoriferae oil, be mixed, be pressed into tablet with above-mentioned granule with this extractum and an amount of magnesium stearate mix homogeneously.

Embodiment seven

Crude drug adopts Radix Salviae Miltiorrhizae 450g, Radix Notoginseng 141g, Styrax 8g, Flos Carthami 50g.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.35～1.39 (55 ℃).Radix Notoginseng powder is broken into fine powder, mixes all with above-mentioned extractum, drying is processed granule.With the Styrax porphyrize, be mixed with above-mentioned granule, be pressed into 1000, or sugar coating, promptly get.

Embodiment eight

Crude drug adopts Radix Salviae Miltiorrhizae 558g, Radix Notoginseng 34g, Styrax 8g, Flos Carthami 72g.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.35～1.39 (55 ℃).Radix Notoginseng powder is broken into fine powder, mixes all,, be mixed, add 3% polyvidone alcoholic solution system soft material, cross 18 mesh sieve system granules with above-mentioned granule with the Styrax porphyrize with above-mentioned extractum, 60 ℃ of dryings 30～45 minutes, granulate, the adding Pulvis Talci, mixing fills in capsule, promptly gets.

Embodiment nine

Crude drug adopts Radix Salviae Miltiorrhizae 360g, Radix Notoginseng 232g, Styrax 8g, Flos Carthami 24g.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.35～1.39 (55 ℃).Drying is processed granule.With the Styrax porphyrize, be mixed with above-mentioned granule, add an amount of magnesium stearate mix homogeneously, be pressed into 1000, or sugar coating, promptly get.

Embodiment ten

Crude drug adopts Radix Salviae Miltiorrhizae 500g, Radix Notoginseng 94g, Styrax 6g, Flos Carthami 42g.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.32～1.40 (55 ℃), add Styrax, mix homogeneously, with the lactose fluidisation, drying is processed granule, promptly gets in the batch (-type) fluid bed.

Embodiment 11

Crude drug adopts Radix Salviae Miltiorrhizae 290g, Radix Notoginseng 306g, Styrax 6g, Flos Carthami 20g.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.32～1.40 (55 ℃), add Styrax, again with mannitol 30g, calcium disodium edetate 5g, distilled water 5ml, behind the said components mixing, lyophilization, 1000 of packing.

Embodiment 12

Crude drug adopts Radix Salviae Miltiorrhizae 210g, Radix Notoginseng 380g, Styrax 4g, Flos Carthami 30g.

Described ultrafiltrate concentrated obtain the extractum that relative density is 1.32～1.40 (55 ℃),, be pressed into tablet this extractum and Styrax and an amount of magnesium stearate mix homogeneously.

Embodiment 13

Described extract concentrated obtain the extractum that relative density is 1.35～1.39 (55 ℃).Radix Notoginseng and the Lignum Dalbergiae Odoriferae oil and Polyethylene Glycol-6000 20g that get extractum, pulverizing are evenly mixed, are heated to 60 ℃ of temperature, change material after 40 minutes, move in the dropping-pill machine jar that jar temperature remains on 90 ℃.In medicine liquid droplet to the 8 ℃ methyl-silicone oil, take out drop pill, oil removing, screen cloth selects ball, processes 1000 drop pill, promptly gets.

Embodiment 14

Crude drug adopts Radix Salviae Miltiorrhizae 558g, Radix Notoginseng 34g, Lignum Dalbergiae Odoriferae oil 8g, Flos Carthami 72g; Adjuvant xylitol 15.0g, starch 5.0g.

Get above-mentioned extractum and Moschus, evenly mixed with adjuvant xylitol and starch, be heated to 65 ℃ of temperature, change material after 30 minutes, move in the dropping-pill machine jar that jar temperature remains on 85 ℃.In medicine liquid droplet to the 8 ℃ liquid paraffin, take out drop pill, oil removing, screen cloth selects ball, promptly gets.The result shows, prepared drop pill rounding, even, consistent, the no adhesion phenomenon of color and luster of size.By measuring under Chinese Pharmacopoeia version in 2000 the method disintegration item, the result does not add the average 2.78min of baffle plate through screen cloth, meets the pharmacopeia regulation this disintegration.

Embodiment 15

Crude drug adopts Radix Salviae Miltiorrhizae 360g, Radix Notoginseng 232g, Lignum Dalbergiae Odoriferae oil 8g, Flos Carthami 24g, adjuvant lactose 15.0g, arabic gum 3.0g.

Get above-mentioned extractum and Lignum Dalbergiae Odoriferae oil, evenly mixed with adjuvant lactose and arabic gum, be heated to 60 ℃ of temperature, change material after 30 minutes, move in the dropping-pill machine jar that jar temperature remains on 85 ℃.In medicine liquid droplet to the 6 ℃ liquid paraffin, take out drop pill, oil removing, screen cloth selects ball, promptly gets.The result shows, prepared drop pill rounding, even, consistent, the no adhesion phenomenon of color and luster of size.By measuring under Chinese Pharmacopoeia version in 2000 the method disintegration item, the result does not add the average 2.61min of baffle plate through screen cloth, meets the pharmacopeia regulation this disintegration.

Embodiment 16

Crude drug adopts Radix Salviae Miltiorrhizae 500g, Radix Notoginseng 94g, Lignum Dalbergiae Odoriferae oil 6g, Flos Carthami 42g, adjuvant xylitol 16.0g, tragakanta 5.0g.

Get above-mentioned extractum and Lignum Dalbergiae Odoriferae, evenly mixed with adjuvant xylitol and tragakanta, be heated to 60 ℃ of temperature, change material after 25 minutes, move in the dropping-pill machine jar that jar temperature remains on 89 ℃.In medicine liquid droplet to the 5 ℃ liquid paraffin, take out drop pill, oil removing, screen cloth selects ball, promptly gets.The result shows, prepared drop pill rounding, even, consistent, the no adhesion phenomenon of color and luster of size.By measuring under Chinese Pharmacopoeia version in 2000 the method disintegration item, the result does not add the average 2.62min of baffle plate through screen cloth, meets the pharmacopeia regulation this disintegration.

Embodiment 17

Crude drug adopts Radix Salviae Miltiorrhizae 290g, Radix Notoginseng 306g, Lignum Dalbergiae Odoriferae oil 6g, Flos Carthami 20g, sucrose ester 10.0g, polyoxyethylene monostearate 8.0g.

Get above-mentioned extractum, Lignum Dalbergiae Odoriferae oil and adjuvant sucrose ester and polyoxyethylene monostearate are mixed evenly to be heated to 60 ℃ of temperature, changes material after 30 minutes, moves in the dropping-pill machine jar that jar temperature remains on 90 ℃.In medicine liquid droplet to the 5 ℃ liquid paraffin, take out drop pill, oil removing, screen cloth selects ball, promptly gets.The result shows, prepared drop pill rounding, even, consistent, the no adhesion phenomenon of color and luster of size.By measuring under Chinese Pharmacopoeia version in 2000 the method disintegration item, the result does not add the average 2.64min of baffle plate through screen cloth, meets the pharmacopeia regulation this disintegration.

Claims

1. Chinese medicine composition of treating myocardial ischemia-reperfusion injury is characterized in that it is to be prepared from following raw materials in weight portion:

Radix Salviae Miltiorrhizae 20～97,

Radix Notoginseng 2～79,

Flos Carthami 3～15,

Lignum Dalbergiae Odoriferae oil or Styrax 0.2～3.

2. Chinese medicine composition as claimed in claim 1 is characterized in that the weight portion proportioning of said raw material is:

Radix Salviae Miltiorrhizae 63.0～94.0,

Radix Notoginseng 4.0～35.0,

Flos Carthami 4～10, Lignum Dalbergiae Odoriferae oil or Styrax 0.5～2.0.

3. Chinese medicine composition as claimed in claim 2 is characterized in that the weight portion proportioning of said raw material is:

Radix Salviae Miltiorrhizae 75.2～90,

Radix Notoginseng 9～23.5,

Flos Carthami 5～8,

Lignum Dalbergiae Odoriferae oil or Styrax 0.5～1.3.

4. like the arbitrary described Chinese medicine composition of claim 1～3, it is characterized in that it can be prepared from following steps:

(a) Radix Salviae Miltiorrhizae, Radix Notoginseng, Flos Carthami are mixed or process water extract or alcohol extract separately;

(b) described extracting solution being carried out predefecation handles;

(c) further described extracting solution is carried out hyperfiltration treatment;

(d) ultrafiltrate is concentrated, add Lignum Dalbergiae Odoriferae oil or Styrax, process preparation by conventional method.

5. Chinese medicine composition as claimed in claim 4 is characterized in that described alcohol extract is to be selected from the following lower alcohol or the extracting solution of its mixture: methanol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butyl alcohol, isobutanol; And/or described alcohol extract is an ethanol extract; And/or

What said step (a) obtained is the ethanol extract of Radix Salviae Miltiorrhizae and Flos Carthami; And/or

What said step (a) obtained is the aqueous extract that Radix Salviae Miltiorrhizae, Radix Notoginseng and Flos Carthami are mixed and made into; And/or

Described predefecation is treated to coarse filtration-adsorption clarification, adsorption clarification-high speed centrifugation, coarse filtration-microfiltration or coarse filtration-precipitate with ethanol; And/or

The used ultrafilter membrane of said hyperfiltration treatment is selected from: cellulose diacetate film, three cellulose acetate membrane, cyanoethyl cellulose film, PS membrane, sulfonated polysulfone membrane, poly (ether sulfone) film, sulfonated polyether sulfone film, polysulfonamides film, phenolphthalein side group polyarylsulfone (PAS) film, polyvinylidene fluoride film, polyacrylonitrile film, polyimide film, cellulose membrane, methyl methacrylate-acrylonitrile copolymer film, polyacrylonitrile/cellulose diacetate blend film; The ultrafilter membrane that dynamically forms, and the Modified Membrane of above-mentioned film; The molecular cut off of its ultrafilter membrane is 6000～80000; And/or the operating procedure condition of said hyperfiltration treatment is following: the inlet pressure of ultrafiltration is 0.1～0.5MPa, and the liquid outlet pressure ratio inlet pressure of ultrafiltration hangs down 0.25～0.5kPa; Feed temperature is 15～50 ℃; The pH value of feed liquid is controlled at 5～9; When feed liquid stock solution is concentrated 1/15～1/5, add water or dilute alcohol solution ultrafiltration 1～2 time again.

6. preparation, said preparation is gone up acceptable auxiliary by the arbitrary described Chinese medicine composition of claim 1～5 and any or various medicaments and is processed.

7. preparation as claimed in claim 6; Said preparation is a drop pill; Wherein said drop pill is prepared from any Chinese medicine composition of claim 1-5 and substrate adjuvant and plasticity adjuvant, and wherein said substrate adjuvant is selected from least a in pharmaceutically useful monosaccharide, oligosaccharide, polysaccharide, sugar ester, sugar alcohol, fruit acid, advanced higher fatty acid derivative, high fatty alcohol, polyhydric alcohol, carbamide, the polyethylene oxide derivant; Said plasticity substrate adjuvant is selected from starch and derivant, arabic gum, dextran, chitin, sesbania gum, carrageenan, Ficus elastica, Furcellaran, tragakanta, carrageenin, tamarind gum, pectin, xanthan gum, alginic acid and salt thereof, dextrin, cyclodextrin, agar, lactose; Polyvinylpyrrolidone, crospolyvinylpyrrolidone, carbomer, polyvinyl alcohol, acrylic resin, poloxamer, silicon dioxide, gelatin, glyceryl monostearate, polyoxyethylene monostearate.

8. preparation as claimed in claim 7 is characterized in that said substrate adjuvant is selected from sorbitol, xylitol, lactose, maltose, sucrose ester, and they contain in the water of crystallization chemical compound one or more; Said plasticity substrate adjuvant is selected from one or more in pregelatinized Starch, carboxymethyl starch, arabic gum, alginic acid, agar, lactose, glyceryl monostearate, polyoxyethylene monostearate, cross-linking sodium carboxymethyl cellulose, the silicon dioxide.

9. preparation as claimed in claim 8; It is characterized in that described adjuvant is lactose and starch; Xylitol and arabic gum, sucrose ester and glyceryl monostearate, sucrose ester and polyoxyethylene monostearate; Sucrose ester, polyoxyethylene monostearate and cross-linking sodium carboxymethyl cellulose, a kind of combination in sucrose ester, polyoxyethylene monostearate, cross-linking sodium carboxymethyl cellulose and the silicon dioxide.

10. preparation as claimed in claim 9 is characterized in that the wherein said substrate adjuvant and the ratio of the weight of medicine are 1: 0.1～1.